MD00G1058000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009277
Physical Location & Seq
Forward (+)
10630957 .. 10634398
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1058000.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGAAGCCCTCGTCGCGGTACAGCTCGTACGATGCGCGTTCCTCGACCTCCTCGCACTTCTCCGACCCCTCGTCTTCCACAGAGCTCAAGCTCCCAGTTTCCTCACGCGCCGTCGTCAAATCCAAGCCCTCGGATCCCAATATCTCCACGATGGTGAAAAAGTTCATGGAAAAGCGGTCGACTTCCAAGGCCAAGCCGATTAACCCGACGGGGCTGGTGATTCCGTCGGATCTGATCGCGGGCGGATTGAAAAAGCCGGCTAAAAACGGGTCGGGATCGAATTTTGCGGCGCTGGGGAGGAAGCTGTTTGGGAAAGGAGCGGGAACGACCTCGTCGGATAAGAAAAAGGAGGTGAAAGCGTTGACTGAGGTAAAAGGGAACACCAGGACGCTGGCCATGGTGTTGAGGAGTGAGAGAGAGCTCTTGAATTTGAATAAGGAGCAAGAAGTTGAAATTGTCGAGCTTAAATTGATGATTCAAGAAAAGAACAGAGAAGTGGATAAACTGAAGGATTTGTGTTTGAAGCAAAGGGAAGAAATCAAATCGTTGAAGAGCGCAATTTTGTTCCCGGATGTTATGAATTCTCAGCTTCAAGAGCTGTTGGAGAAGCAGGGGTCAGAACTGAAGCAAGCAAAACAAGTAATCCCAAATCTTCAAAGGCAGGTTTGTTCTCTTACCGGTCAGCTTCAGTGCCTTGCAGAGGATCTTGCAGAGGTGAAGGCAGATAAAGGTTCAGTTGGGGCATGTCTTCGACGTGATGACACTTCTCCAAGAACTCCAACTTATGATGACAAAGAATACTCTAATTCTCTTGAGTTCAGCTCTGGTGAACCAGCCAGCCCTGGTAGTCCAGATGACATGTTCCTGAAGGATTTGAATCCTTGCTTGACCCCATTTCCAAAAACAAAATCCAAGGAATTTGATGAGATGGGCTACAACTCCCCATATCGACATGATGAAAGCTTATCTGAAAACAATATGGAGTTTGGATTTAACTCTTGTTCCAGGAAGTTGTCCAGAAGTTCTGATTGTTGCCAGAAGACAGCAACAGAAAGCAGAATAGCACAAGGAAATAGGAGATCGGGTGATGCCAAACAAACTCATGGAAAACAAATTCACCGTAGATTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

378

Amino Acids

41.8

Weight (kDa)

9.29

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 179
AccII CGCG 4 cut(s) 16, 37, 108, 239
AciI CCGC 6 cut(s) 16, 175, 239, 243, 287, 320
AclWI GGATC 5 cut(s) 128, 141, 237, 283, 711
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 5 cut(s) 280, 427, 580, 917, 1113
AcuI CTGAAG 4 cut(s) 527, 644, 671, 887
AfaI GTAC 2 cut(s) 20, 29
AfiI CCNNNNNNNGG 2 cut(s) 15, 700
AflIII ACRYGT 1 cut(s) 858
AgeI ACCGGT 1 cut(s) 677
AhdI GACNNNNNGTC 1 cut(s) 70
AjiI CACGTC 1 cut(s) 755
AjnI CCWGG 3 cut(s) 383, 841, 1004
AleI CACNNNNGTG 1 cut(s) 152
Alw21I GWGCWC 2 cut(s) 87, 423
AlwI GGATC 5 cut(s) 128, 141, 237, 283, 711
AoxI GGCC 2 cut(s) 189, 393
ApoI RAATTY 5 cut(s) 280, 427, 580, 917, 1113
AsiGI ACCGGT 1 cut(s) 677
Asp700I GAANNNNTTC 1 cut(s) 161
AspLEI GCGC 4 cut(s) 37, 110, 292, 557
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 7 cut(s) 166, 229, 364, 727, 839, 1097, 1109
BalI TGGCCA 1 cut(s) 395
BamHI GGATCC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 87, 423
BbsI GAAGAC 3 cut(s) 66, 740, 1046
Bbv12I GWGCWC 2 cut(s) 87, 423
BccI CCATC 2 cut(s) 145, 922
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 3 cut(s) 385, 843, 1006
BcnI CCSGG 1 cut(s) 569
BfaI CTAG 1 cut(s) 1132
BfoI RGCGCY 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 652
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 4 cut(s) 385, 569, 843, 1006
BmeRI GACNNNNNGTC 1 cut(s) 70
BmgBI CACGTC 1 cut(s) 755
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 4 cut(s) 385, 569, 843, 1006
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 22, 1078
BmuI ACTGGG 1 cut(s) 89
BpiI GAAGAC 3 cut(s) 66, 740, 1046
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpuEI CTTGAG 2 cut(s) 71, 833
BpuMI CCSGG 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 876
BsaJI CCNNGG 5 cut(s) 129, 186, 396, 841, 912
BsaWI WCCGGW 1 cut(s) 677
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 700
Bse118I RCCGGY 2 cut(s) 256, 677
Bse1I ACTGG 1 cut(s) 95
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 3 cut(s) 385, 843, 1006
BseDI CCNNGG 5 cut(s) 129, 186, 396, 841, 912
BseGI GGATG 1 cut(s) 577
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 2 cut(s) 15, 700
BseMII CTCAG 2 cut(s) 357, 599
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 2 cut(s) 40, 421
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 4 cut(s) 16, 37, 108, 239
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 2 cut(s) 191, 395
BshTI ACCGGT 1 cut(s) 677
BsiEI CGRYCG 1 cut(s) 179
BsiHKAI GWGCWC 2 cut(s) 87, 423
BsiSI CCGG 3 cut(s) 257, 569, 678
BsiWI CGTACG 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 15, 700
BsnI GGCC 2 cut(s) 191, 395
Bsp1286I GDGCHC 2 cut(s) 87, 423
Bsp143I GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 6 cut(s) 16, 175, 239, 243, 287, 320
BspANI GGCC 2 cut(s) 191, 395
BspCNI CTCAG 2 cut(s) 358, 598
BspFNI CGCG 4 cut(s) 16, 37, 108, 239
BspLI GGNNCC 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 5 cut(s) 128, 141, 237, 283, 711
BspQI GCTCTTC 1 cut(s) 545
BsrBI CCGCTC 1 cut(s) 320
BsrFI RCCGGY 2 cut(s) 256, 677
BsrI ACTGG 1 cut(s) 95
BssAI RCCGGY 2 cut(s) 256, 677
BssECI CCNNGG 5 cut(s) 129, 186, 396, 841, 912
BssMI GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
BssT1I CCWWGG 3 cut(s) 186, 396, 912
Bst2UI CCWGG 3 cut(s) 385, 843, 1006
Bst4CI ACNGT 1 cut(s) 1121
Bst6I CTCTTC 1 cut(s) 545
BstC8I GCNNGC 5 cut(s) 241, 258, 393, 630, 838
BstDEI CTNAG 2 cut(s) 366, 585
BstDSI CCRYGG 1 cut(s) 396
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 4 cut(s) 16, 37, 108, 239
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 4 cut(s) 37, 110, 292, 557
BstKTI GATC 6 cut(s) 136, 232, 237, 278, 706, 1082
BstMBI GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 13, 595
BstNI CCWGG 3 cut(s) 385, 843, 1006
BstNSI RCATGY 2 cut(s) 747, 862
BstSCI CCNGG 4 cut(s) 383, 567, 841, 1004
BstUI CGCG 4 cut(s) 16, 37, 108, 239
BstV2I GAAGAC 3 cut(s) 66, 740, 1046
BstX2I RGATCY 3 cut(s) 133, 229, 703
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 3 cut(s) 133, 229, 703
BsuRI GGCC 2 cut(s) 191, 395
BtgI CCRYGG 1 cut(s) 396
BtrI CACGTC 1 cut(s) 755
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 1 cut(s) 695
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 5 cut(s) 241, 258, 393, 630, 838
CfoI GCGC 4 cut(s) 37, 110, 292, 557
Cfr10I RCCGGY 2 cut(s) 256, 677
CseI GACGC 1 cut(s) 397
Csp6I GTAC 2 cut(s) 19, 28
CspAI ACCGGT 1 cut(s) 677
CviAII CATG 6 cut(s) 166, 397, 744, 859, 953, 1103
CviQI GTAC 2 cut(s) 19, 28
DdeI CTNAG 2 cut(s) 366, 585
DpnI GATC 6 cut(s) 135, 231, 236, 277, 705, 1081
DpnII GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
DriI GACNNNNNGTC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 545
Eam1105I GACNNNNNGTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 545
EciI GGCGGA 1 cut(s) 258
Ecl136II GAGCTC 2 cut(s) 85, 421
Eco130I CCWWGG 3 cut(s) 186, 396, 912
Eco24I GRGCYC 2 cut(s) 87, 423
Eco53kI GAGCTC 2 cut(s) 85, 421
Eco57I CTGAAG 4 cut(s) 527, 644, 671, 887
EcoICRI GAGCTC 2 cut(s) 85, 421
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 3 cut(s) 383, 841, 1004
EcoT14I CCWWGG 3 cut(s) 186, 396, 912
EcoT38I GRGCYC 2 cut(s) 87, 423
ErhI CCWWGG 3 cut(s) 186, 396, 912
FaeI CATG 6 cut(s) 169, 400, 747, 862, 956, 1106
FatI CATG 6 cut(s) 165, 396, 743, 858, 952, 1102
FauI CCCGC 2 cut(s) 232, 313
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 2 cut(s) 87, 423
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 1132
GlaI GCGC 4 cut(s) 36, 109, 291, 556
GluI GCNGC 1 cut(s) 288
GsaI CCCAGC 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 2 cut(s) 191, 395
HapII CCGG 3 cut(s) 257, 569, 678
HgaI GACGC 1 cut(s) 397
HhaI GCGC 4 cut(s) 37, 110, 292, 557
Hin1II CATG 6 cut(s) 169, 400, 747, 862, 956, 1106
Hin6I GCGC 4 cut(s) 35, 108, 290, 555
HinP1I GCGC 4 cut(s) 35, 108, 290, 555
HincII GTYRAC 2 cut(s) 180, 363
HindII GTYRAC 2 cut(s) 180, 363
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 3 cut(s) 220, 475, 877
HpaII CCGG 3 cut(s) 257, 569, 678
HphI GGTGA 7 cut(s) 166, 229, 364, 727, 839, 1097, 1109
Hpy166II GTNNAC 3 cut(s) 180, 363, 830
Hpy188I TCNGA 8 cut(s) 64, 133, 229, 234, 337, 619, 970, 1027
Hpy188III TCNNGA 8 cut(s) 273, 424, 479, 593, 812, 851, 865, 1017
Hpy8I GTNNAC 3 cut(s) 180, 363, 830
Hpy99I CGWCG 6 cut(s) 16, 116, 211, 229, 337, 756
HpyAV CCTTC 3 cut(s) 502, 712, 862
HpyCH4III ACNGT 1 cut(s) 1121
HpyCH4IV ACGT 1 cut(s) 754
HpyCH4V TGCA 2 cut(s) 698, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 595
HpyF3I CTNAG 2 cut(s) 366, 585
HpySE526I ACGT 1 cut(s) 754
Hsp92II CATG 6 cut(s) 169, 400, 747, 862, 956, 1106
HspAI GCGC 4 cut(s) 35, 108, 290, 555
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Kzo9I GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 3 cut(s) 96, 317, 439
LweI GCATC 2 cut(s) 22, 1078
MaeI CTAG 1 cut(s) 1132
MaeII ACGT 1 cut(s) 754
MalI GATC 6 cut(s) 135, 231, 236, 277, 705, 1081
MbiI CCGCTC 1 cut(s) 320
MboI GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
MboII GAAGA 6 cut(s) 66, 545, 562, 644, 740, 1051
MflI RGATCY 3 cut(s) 133, 229, 703
MhlI GDGCHC 2 cut(s) 87, 423
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 9 cut(s) 280, 427, 453, 467, 558, 580, 805, 917, 1113
MluNI TGGCCA 1 cut(s) 395
MmeI TCCRAC 5 cut(s) 87, 207, 315, 582, 803
Mox20I TGGCCA 1 cut(s) 395
MroNI GCCGGC 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 201, 465, 993
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 3 cut(s) 257, 569, 678
MspR9I CCNGG 4 cut(s) 385, 569, 843, 1006
MvaI CCWGG 3 cut(s) 385, 843, 1006
MvnI CGCG 4 cut(s) 16, 37, 108, 239
MwoI GCNNNNNNNGC 2 cut(s) 13, 595
NaeI GCCGGC 1 cut(s) 258
NciI CCSGG 1 cut(s) 569
NcoI CCATGG 1 cut(s) 396
NdeII GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
NgoMIV GCCGGC 1 cut(s) 256
NlaIII CATG 6 cut(s) 169, 400, 747, 862, 956, 1106
NlaIV GGNNCC 1 cut(s) 135
NspI RCATGY 2 cut(s) 747, 862
OliI CACNNNNGTG 1 cut(s) 152
PciI ACATGT 1 cut(s) 858
PciSI GCTCTTC 1 cut(s) 545
PdiI GCCGGC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 3 cut(s) 220, 475, 877
Pfl23II CGTACG 1 cut(s) 27
PflFI GACNNNGTC 1 cut(s) 331
PfoI TCCNGGA 2 cut(s) 567, 1004
PinAI ACCGGT 1 cut(s) 677
PkrI GCNGC 1 cut(s) 289
PscI ACATGT 1 cut(s) 858
Psp124BI GAGCTC 2 cut(s) 87, 423
Psp6I CCWGG 3 cut(s) 383, 841, 1004
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 3 cut(s) 383, 841, 1004
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 135
PsuI RGATCY 3 cut(s) 133, 229, 703
PsyI GACNNNGTC 1 cut(s) 331
RsaI GTAC 2 cut(s) 20, 29
RsaNI GTAC 2 cut(s) 19, 28
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 2 cut(s) 87, 423
SalI GTCGAC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 3 cut(s) 201, 465, 993
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 6 cut(s) 133, 229, 234, 275, 703, 1079
ScrFI CCNGG 4 cut(s) 385, 569, 843, 1006
SduI GDGCHC 2 cut(s) 87, 423
SfaNI GCATC 2 cut(s) 22, 1078
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 86, 812
SmoI CTYRAG 2 cut(s) 86, 812
Sse9I AATT 9 cut(s) 280, 427, 453, 467, 558, 580, 805, 917, 1113
SsiI CCGC 6 cut(s) 16, 175, 239, 243, 287, 320
SspMI CTAG 1 cut(s) 1132
SstI GAGCTC 2 cut(s) 87, 423
StyD4I CCNGG 4 cut(s) 383, 567, 841, 1004
StyI CCWWGG 3 cut(s) 186, 396, 912
TaaI ACNGT 1 cut(s) 1121
TaiI ACGT 1 cut(s) 757
TaqI TCGA 6 cut(s) 44, 179, 278, 459, 751, 949
TasI AATT 9 cut(s) 280, 427, 453, 467, 558, 580, 805, 917, 1113
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 3 cut(s) 220, 475, 877
Tru1I TTAA 3 cut(s) 201, 465, 993
Tru9I TTAA 3 cut(s) 201, 465, 993
TscAI CASTG 1 cut(s) 695
TspDTI ATGAA 4 cut(s) 17, 154, 593, 972
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 695
Tth111I GACNNNGTC 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 5 cut(s) 280, 427, 580, 917, 1113
XceI RCATGY 2 cut(s) 747, 862
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.