pycom10g28620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009277
Physical Location & Seq
Reverse (-)
29151956 .. 29154294
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g28620.3

Sequence Viewer

Length: 1260 bp
ATGAAGCCCTCGTCGCGGTACAGCTCGTACGATGCCCGCTCCTCAACCTCCTCGCACTTCTCCGACCCCTCGTCTTCCAGAGAGCTCAAGCACCCCGCTTCCTCACGCGCCGTCGTCAAATCCAAGCCGTCGGATCCCAACATCTCCACGATGGTGAAAAAGTTCATGGAAAAGCGGTCGACGTCCAAGCCCAAGCCGATTAACCCGACGGGATTGGTTATTCCGTCGGATCTGATCGCCGGCGGGTTGAAAAAGCAGGCTAAAAACGGGTCGGGTTCGAATTTCGCGGCGCTTGGTAGGAAGCTGTTTGGAAAAGAAGCGGGAACGGCGTCGTCGGATAAGAAGAAGGAGATGAAGGCCTTGACTGAGGTCAAAGGGAACACCAGGACGCTGGCTATGGTGTTGAGGAGTGAGAGAGAGCTCTTGAATTCGAATAAGGAGCAAGAAGTTGAGATTACCGAGCTTAAATTAATAATTCAAGAAAAGAACAGAGAAGTGGATAAACTGAAGGATTTGTGTTTGAAGCAAAGGGAAGAAATCAAATCGTTGAAGAGCGCGATTTTGTTCCCGGATGTTATGAATTCTCAGCTTCAAGAGCTGTTGGAGAAGCAGGGGTCAGAGCTGAAGCAAGCAAAACAAGTAATCCCAAATCTTCAAAGGCAGGTCACTTCTCTTACTGGTCAGCTTCAGTGCCTTGCGGAGGATCTTGCTGAGGTGAAGGCTGACAAGGGTTCAGTTAGGGTGTGTTTTCAACGTGGTGACACTTCTCCAAGAACCCCAACATATGATGACAAAGAATTCTCTAATTCTCTTGAGCTCATCTCTGATGATCTGGCCAGCCCTGGTAGTCCAGATGACCTGTTCCTGAAGGATTTAAATCCTTGCTTGACCCCATATGCAAAAACAAAGTCCAAGGAATTTGACGAGATGGGCTATGACTCTCCATATCGACATGATGAAAGCTTATCCGAAAACAATATGGAGTTTGGATTTAACTCTTGTTCCAGGAAGTTGTCCAAAAGTTCTGATTGTTGCCAGAAGCCCGAAACAGAAAGCAGATTAGCTCAAGGAAATCGGAGATCGGGTGATGCCAAACGAACTTATGGAAAACAAATTCATCGTAAATTTATCTCGATCAAAGGTCATATGGTTTCTATAAGTCATCTGTTTCCTTCTGTTATTTTTCCATCTTTTGTTGCATCTGGGAAGTATTTTTCCATCTTTTGTTGCAGTAGAAAAGTTTGTACACGGTGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

420

Amino Acids

46.72

Weight (kDa)

9.33

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 185
Acc36I ACCTGC 1 cut(s) 652
AccB1I GGYRCC 1 cut(s) 1251
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 179
AccII CGCG 4 cut(s) 16, 108, 287, 557
AciI CCGC 8 cut(s) 16, 37, 96, 175, 243, 287, 320, 698
AclWI GGATC 4 cut(s) 128, 141, 237, 711
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 7 cut(s) 280, 427, 580, 797, 917, 1113, 1124
AcuI CTGAAG 4 cut(s) 527, 644, 671, 887
AcyI GRCGYC 2 cut(s) 182, 329
AfaI GTAC 3 cut(s) 20, 29, 1246
AfiI CCNNNNNNNGG 2 cut(s) 15, 700
AgsI TTSAA 8 cut(s) 250, 427, 479, 523, 550, 593, 656, 752
AhdI GACNNNNNGTC 1 cut(s) 70
AjnI CCWGG 3 cut(s) 383, 841, 1004
AleI CACNNNNGTG 1 cut(s) 152
Alw21I GWGCWC 3 cut(s) 87, 423, 819
AlwI GGATC 4 cut(s) 128, 141, 237, 711
AoxI GGCC 2 cut(s) 357, 834
ApoI RAATTY 7 cut(s) 280, 427, 580, 797, 917, 1113, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 893, 925
AseI ATTAAT 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 161
AspLEI GCGC 3 cut(s) 110, 292, 557
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 4 cut(s) 166, 727, 770, 1097
AsuII TTCGAA 2 cut(s) 278, 431
BaeGI GKGCMC 1 cut(s) 1256
BalI TGGCCA 1 cut(s) 836
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 1251
BanII GRGCYC 3 cut(s) 87, 423, 819
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 3 cut(s) 87, 423, 819
BbvCI CCTCAGC 1 cut(s) 711
BccI CCATC 4 cut(s) 145, 922, 1195, 1226
BceAI ACGGC 3 cut(s) 95, 112, 342
BciT130I CCWGG 3 cut(s) 385, 843, 1006
BcnI CCSGG 1 cut(s) 569
BfoI RGCGCY 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 652
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 4 cut(s) 385, 569, 843, 1006
BmeRI GACNNNNNGTC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 135, 1253
BmrFI CCNGG 4 cut(s) 385, 569, 843, 1006
BmsI GCATC 3 cut(s) 22, 1078, 1208
BoxI GACNNNNGTC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 806, 838
Bpu10I CCTNAGC 1 cut(s) 711
Bpu14I TTCGAA 2 cut(s) 278, 431
BpuEI CTTGAG 3 cut(s) 71, 833, 1050
BpuMI CCSGG 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 876
BsaHI GRCGYC 2 cut(s) 182, 329
BsaJI CCNNGG 2 cut(s) 841, 912
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 700
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 682
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 3 cut(s) 385, 843, 1006
BseDI CCNNGG 2 cut(s) 841, 912
BseGI GGATG 1 cut(s) 577
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 2 cut(s) 15, 700
BseMII CTCAG 3 cut(s) 357, 599, 702
BseNI ACTGG 1 cut(s) 682
BseRI GAGGAG 3 cut(s) 31, 40, 421
BseSI GKGCMC 1 cut(s) 1256
Bsh1236I CGCG 4 cut(s) 16, 108, 287, 557
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 2 cut(s) 359, 836
BshNI GGYRCC 1 cut(s) 1251
BsiEI CGRYCG 1 cut(s) 179
BsiHKAI GWGCWC 3 cut(s) 87, 423, 819
BsiSI CCGG 2 cut(s) 240, 569
BsiWI CGTACG 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 15, 700
BsnI GGCC 2 cut(s) 359, 836
Bsp119I TTCGAA 2 cut(s) 278, 431
Bsp1286I GDGCHC 4 cut(s) 87, 423, 819, 1256
Bsp1407I TGTACA 1 cut(s) 1244
Bsp143I GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
BspACI CCGC 8 cut(s) 16, 37, 96, 175, 243, 287, 320, 698
BspANI GGCC 2 cut(s) 359, 836
BspCNI CTCAG 3 cut(s) 358, 598, 703
BspFNI CGCG 4 cut(s) 16, 108, 287, 557
BspLI GGNNCC 2 cut(s) 135, 1253
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 4 cut(s) 128, 141, 237, 711
BspQI GCTCTTC 1 cut(s) 545
BspT104I TTCGAA 2 cut(s) 278, 431
BspT107I GGYRCC 1 cut(s) 1251
BsrBI CCGCTC 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 239
BsrGI TGTACA 1 cut(s) 1244
BsrI ACTGG 1 cut(s) 682
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 841, 912
BssMI GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
BssNI GRCGYC 2 cut(s) 182, 329
BssT1I CCWWGG 1 cut(s) 912
Bst2UI CCWGG 3 cut(s) 385, 843, 1006
Bst4CI ACNGT 1 cut(s) 1251
Bst6I CTCTTC 1 cut(s) 545
BstACI GRCGYC 2 cut(s) 182, 329
BstAUI TGTACA 1 cut(s) 1244
BstBI TTCGAA 2 cut(s) 278, 431
BstC8I GCNNGC 6 cut(s) 37, 241, 258, 393, 630, 838
BstDEI CTNAG 3 cut(s) 366, 585, 711
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 4 cut(s) 16, 108, 287, 557
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 3 cut(s) 110, 292, 557
BstKTI GATC 7 cut(s) 136, 232, 237, 706, 832, 1082, 1137
BstMBI GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 3 cut(s) 13, 326, 595
BstNI CCWGG 3 cut(s) 385, 843, 1006
BstPAI GACNNNNGTC 1 cut(s) 368
BstSCI CCNGG 4 cut(s) 383, 567, 841, 1004
BstSLI GKGCMC 1 cut(s) 1256
BstUI CGCG 4 cut(s) 16, 108, 287, 557
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 3 cut(s) 133, 229, 703
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 3 cut(s) 133, 229, 703
BsuRI GGCC 2 cut(s) 359, 836
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 1 cut(s) 695
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 6 cut(s) 37, 241, 258, 393, 630, 838
CfoI GCGC 3 cut(s) 110, 292, 557
Cfr10I RCCGGY 1 cut(s) 239
CseI GACGC 2 cut(s) 318, 397
Csp6I GTAC 3 cut(s) 19, 28, 1245
CviAII CATG 2 cut(s) 166, 953
CviQI GTAC 3 cut(s) 19, 28, 1245
DdeI CTNAG 3 cut(s) 366, 585, 711
DpnI GATC 7 cut(s) 135, 231, 236, 705, 831, 1081, 1136
DpnII GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
DraI TTTAAA 1 cut(s) 876
DriI GACNNNNNGTC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 545
Eam1105I GACNNNNNGTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 545
Ecl136II GAGCTC 3 cut(s) 85, 421, 817
Eco130I CCWWGG 1 cut(s) 912
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 3 cut(s) 87, 423, 819
Eco53kI GAGCTC 3 cut(s) 85, 421, 817
Eco57I CTGAAG 4 cut(s) 527, 644, 671, 887
EcoICRI GAGCTC 3 cut(s) 85, 421, 817
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoRI GAATTC 3 cut(s) 427, 580, 797
EcoRII CCWGG 3 cut(s) 383, 841, 1004
EcoT14I CCWWGG 1 cut(s) 912
EcoT38I GRGCYC 3 cut(s) 87, 423, 819
ErhI CCWWGG 1 cut(s) 912
FaeI CATG 2 cut(s) 169, 956
FatI CATG 2 cut(s) 165, 952
FauI CCCGC 4 cut(s) 44, 103, 236, 313
FauNDI CATATG 3 cut(s) 784, 895, 1146
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 3 cut(s) 87, 423, 819
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 3 cut(s) 109, 291, 556
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 2 cut(s) 359, 836
HapII CCGG 2 cut(s) 240, 569
HgaI GACGC 2 cut(s) 318, 397
HhaI GCGC 3 cut(s) 110, 292, 557
Hin1I GRCGYC 2 cut(s) 182, 329
Hin1II CATG 2 cut(s) 169, 956
Hin6I GCGC 3 cut(s) 108, 290, 555
HinP1I GCGC 3 cut(s) 108, 290, 555
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 1 cut(s) 938
HpaII CCGG 2 cut(s) 240, 569
HphI GGTGA 4 cut(s) 166, 727, 770, 1097
Hpy166II GTNNAC 2 cut(s) 180, 1247
Hpy188III TCNNGA 8 cut(s) 78, 424, 479, 593, 812, 851, 865, 1132
Hpy8I GTNNAC 2 cut(s) 180, 1247
Hpy99I CGWCG 8 cut(s) 16, 116, 133, 184, 211, 229, 334, 337
HpyAV CCTTC 6 cut(s) 340, 349, 502, 712, 862, 1182
HpyCH4III ACNGT 1 cut(s) 1251
HpyCH4IV ACGT 2 cut(s) 182, 754
HpyCH4V TGCA 3 cut(s) 899, 1199, 1230
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 326, 595
HpyF3I CTNAG 3 cut(s) 366, 585, 711
HpySE526I ACGT 2 cut(s) 182, 754
Hsp92I GRCGYC 2 cut(s) 182, 329
Hsp92II CATG 2 cut(s) 169, 956
HspAI GCGC 3 cut(s) 108, 290, 555
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 2 cut(s) 44, 439
LweI GCATC 3 cut(s) 22, 1078, 1208
MaeII ACGT 2 cut(s) 182, 754
MaeIII GTNAC 2 cut(s) 664, 758
MalI GATC 7 cut(s) 135, 231, 236, 705, 831, 1081, 1136
MbiI CCGCTC 1 cut(s) 39
MboI GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
MboII GAAGA 5 cut(s) 66, 355, 545, 562, 644
MflI RGATCY 3 cut(s) 133, 229, 703
MhlI GDGCHC 4 cut(s) 87, 423, 819, 1256
MlsI TGGCCA 1 cut(s) 836
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 1 cut(s) 932
MmeI TCCRAC 5 cut(s) 87, 111, 207, 315, 582
Mox20I TGGCCA 1 cut(s) 836
MreI CGCCGGCG 1 cut(s) 239
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 836
MseI TTAA 5 cut(s) 201, 465, 470, 875, 993
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 836
MspI CCGG 2 cut(s) 240, 569
MspR9I CCNGG 4 cut(s) 385, 569, 843, 1006
MvaI CCWGG 3 cut(s) 385, 843, 1006
MvnI CGCG 4 cut(s) 16, 108, 287, 557
MwoI GCNNNNNNNGC 3 cut(s) 13, 326, 595
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 569
NdeI CATATG 3 cut(s) 784, 895, 1146
NdeII GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 169, 956
NlaIV GGNNCC 2 cut(s) 135, 1253
NmuCI GTSAC 2 cut(s) 664, 758
NspV TTCGAA 2 cut(s) 278, 431
OliI CACNNNNGTG 1 cut(s) 152
PceI AGGCCT 1 cut(s) 359
PciSI GCTCTTC 1 cut(s) 545
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 161
Pfl23II CGTACG 1 cut(s) 27
PfoI TCCNGGA 2 cut(s) 567, 1004
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 932
PpsI GAGTC 1 cut(s) 932
PshAI GACNNNNGTC 1 cut(s) 368
PshBI ATTAAT 1 cut(s) 470
Psp124BI GAGCTC 3 cut(s) 87, 423, 819
Psp6I CCWGG 3 cut(s) 383, 841, 1004
PspGI CCWGG 3 cut(s) 383, 841, 1004
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 135, 1253
PsuI RGATCY 3 cut(s) 133, 229, 703
RsaI GTAC 3 cut(s) 20, 29, 1246
RsaNI GTAC 3 cut(s) 19, 28, 1245
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 3 cut(s) 87, 423, 819
SalI GTCGAC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 5 cut(s) 201, 465, 470, 875, 993
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 7 cut(s) 133, 229, 234, 703, 829, 1079, 1134
SchI GAGTC 1 cut(s) 932
ScrFI CCNGG 4 cut(s) 385, 569, 843, 1006
SduI GDGCHC 4 cut(s) 87, 423, 819, 1256
SfaNI GCATC 3 cut(s) 22, 1078, 1208
SfuI TTCGAA 2 cut(s) 278, 431
SgrAI CRCCGGYG 1 cut(s) 239
SmiI ATTTAAAT 1 cut(s) 876
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 3 cut(s) 86, 812, 1065
SmoI CTYRAG 3 cut(s) 86, 812, 1065
SseBI AGGCCT 1 cut(s) 359
SsiI CCGC 8 cut(s) 16, 37, 96, 175, 243, 287, 320, 698
SstI GAGCTC 3 cut(s) 87, 423, 819
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 4 cut(s) 383, 567, 841, 1004
StyI CCWWGG 1 cut(s) 912
SwaI ATTTAAAT 1 cut(s) 876
TaaI ACNGT 1 cut(s) 1251
TaiI ACGT 2 cut(s) 185, 757
TaqI TCGA 5 cut(s) 179, 278, 431, 949, 1133
TatI WGTACW 1 cut(s) 1244
TauI GCSGC 1 cut(s) 290
Tru1I TTAA 5 cut(s) 201, 465, 470, 875, 993
Tru9I TTAA 5 cut(s) 201, 465, 470, 875, 993
TscAI CASTG 1 cut(s) 695
TseFI GTSAC 2 cut(s) 664, 758
Tsp45I GTSAC 2 cut(s) 664, 758
TspDTI ATGAA 6 cut(s) 17, 154, 368, 593, 972, 1106
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 695
VspI ATTAAT 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 7 cut(s) 280, 427, 580, 797, 917, 1113, 1124
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 161
ZraI GACGTC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.