Rw5G000640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009277
Physical Location & Seq
Forward (+)
442392 .. 444635
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000640.1

Sequence Viewer

Length: 1140 bp
ATGAAGCCCTCGTCGCGCTACAGCTCCTACGATGCACGCTCCTCCACCGCGTCCCACCACTCCTACCAGTCCTCCTCTGCCGAATACAAGCCCAAGCACCCCGCCACGTCCTCCTCACGCGCCGTCGTCAAATCCAAGCCCTCCCCGGATCCCAACCTCACCACCCTGGTGAAAAAGTTCATGGAAAAGCGCTCCTCCTCCAAGCCCAAGCCGATTAACGGCACCGCCTTGGTGATTCCGTCCGATGTGATCGCCGACGACCTGAAAAAGACGGCAAAAAATGGCTCCAACTTCGCGGCGCTGCACAGGAAGCTCTTCGGGAAAGCCACGGGATCGGCGGACAAGAAGAAGGAGGTCAAGGCGTTGACCGAGGTCAAAGGGAACACCAGGACGTTGGCCATGGTGTTGAGAAGCGAGAGAGAGCTTCTCAGTCTGAATAAGGGCCAAGAATCGGACATCGCCGAGCTCAGGTTGATGCTCGAAGAGAAGAACAAAGAAGTGGACAAATTGAAGGACTTGTGTTTGAAGCAAAGGGAGGAAATCAAATCGTTGAAGAGCACGATTTTGTTCCCGGATGTTATGAATTCTCAGCTTCAAGAGTTGCTAGAGAAGCAAGGGTCGGAGCTGAAGCAAGCCAAACAAGTAATCCCAAATCTTCAAAGGCAGGTCACTTCTCTCACTGGCCAGCTCCAATGCCTTGCAGTGGATCTTGCTGAGGTGAAAGCAGACAAACATTCGGTTAGGGCATGTTTTCAAAGTGATGACAGTATTCCCAGAACTCCAACGTATCATAAGAAAGAATCTTTGAATTCTCTGGAGTTCAGCTCTTGTGATCCAACCAGCCCTGGTAGTCCAGATGACATGTTCCTAAAGGATTTAAATCCATGCTTAACACCTTTCTGTGCTAAATCAAAGTCCAAGGAATTTGATGAGATGGGTTACTACTCTCCACATGGCTATGATGAAAGCTTATCTAAAAACAACGTGGAGTTTGGATTTAACTCTTGTGCGAGGAAGTTGACCCGAAGTTCTGACTGCTGCCAAAATTCTGAAACAGAAAGTAGAACAGCTCATGCAAATCAGAGATCTGGTGATGCCCAACGAACATATGGCAATCGAACTCACCGTAATTATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

42.3

Weight (kDa)

9.23

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 655
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 4 cut(s) 16, 50, 120, 296
AciI CCGC 5 cut(s) 48, 102, 225, 296, 338
AclWI GGATC 5 cut(s) 143, 156, 340, 714, 827
AcoI YGGCCR 2 cut(s) 396, 682
AcsI RAATTY 4 cut(s) 583, 808, 923, 1045
AcuI CTGAAG 1 cut(s) 647
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 4 cut(s) 218, 451, 468, 703
AflIII ACRYGT 1 cut(s) 861
AgsI TTSAA 7 cut(s) 511, 526, 553, 596, 659, 755, 808
AjiI CACGTC 1 cut(s) 108
AjnI CCWGG 3 cut(s) 165, 386, 844
AleI CACNNNNGTG 1 cut(s) 167
Alw21I GWGCWC 2 cut(s) 468, 560
AlwI GGATC 5 cut(s) 143, 156, 340, 714, 827
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 3 cut(s) 396, 442, 682
ApeKI GCWGC 2 cut(s) 301, 1038
ApoI RAATTY 4 cut(s) 583, 808, 923, 1045
Asp700I GAANNNNTTC 2 cut(s) 176, 314
AspLEI GCGC 4 cut(s) 18, 122, 192, 301
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 2 cut(s) 146, 572
AsuHPI GGTGA 6 cut(s) 151, 181, 244, 730, 1103, 1115
BalI TGGCCA 2 cut(s) 398, 684
BamHI GGATCC 1 cut(s) 148
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 468
Bbv12I GWGCWC 2 cut(s) 468, 560
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 2 cut(s) 288, 1025
BccI CCATC 1 cut(s) 928
BceAI ACGGC 3 cut(s) 107, 235, 288
BciT130I CCWGG 3 cut(s) 167, 388, 846
BcnI CCSGG 2 cut(s) 146, 572
BfaI CTAG 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 19
BfoI RGCGCY 2 cut(s) 193, 302
BfuAI ACCTGC 1 cut(s) 655
BglII AGATCT 1 cut(s) 1085
BisI GCNGC 3 cut(s) 297, 302, 1039
BlsI GCNGC 3 cut(s) 298, 303, 1040
Bme1390I CCNGG 5 cut(s) 146, 167, 388, 572, 846
BmgBI CACGTC 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 3 cut(s) 150, 223, 286
BmrFI CCNGG 5 cut(s) 146, 167, 388, 572, 846
BmsI GCATC 3 cut(s) 22, 465, 1084
BoxI GACNNNNGTC 1 cut(s) 371
BplI GAGNNNNNCTC 4 cut(s) 411, 443, 809, 841
BpmI CTGGAG 1 cut(s) 836
Bpu10I CCTNAGC 2 cut(s) 467, 714
BpuMI CCSGG 2 cut(s) 146, 572
BsaBI GATNNNNATC 1 cut(s) 879
BsaJI CCNNGG 8 cut(s) 144, 165, 228, 327, 369, 399, 844, 918
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 451, 468, 703
Bse1I ACTGG 2 cut(s) 67, 685
Bse8I GATNNNNATC 1 cut(s) 879
BseBI CCWGG 3 cut(s) 167, 388, 846
BseDI CCNNGG 8 cut(s) 144, 165, 228, 327, 369, 399, 844, 918
BseGI GGATG 1 cut(s) 580
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 4 cut(s) 218, 451, 468, 703
BseMII CTCAG 4 cut(s) 442, 481, 602, 705
BseNI ACTGG 2 cut(s) 67, 685
BseRI GAGGAG 5 cut(s) 31, 64, 103, 184, 187
BseXI GCAGC 2 cut(s) 288, 1025
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 4 cut(s) 16, 50, 120, 296
BshFI GGCC 3 cut(s) 398, 444, 684
BshNI GGYRCC 1 cut(s) 221
BsiHKAI GWGCWC 2 cut(s) 468, 560
BsiSI CCGG 2 cut(s) 146, 572
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 4 cut(s) 218, 451, 468, 703
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 3 cut(s) 398, 444, 684
Bsp1286I GDGCHC 2 cut(s) 468, 560
Bsp143I GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 5 cut(s) 48, 102, 225, 296, 338
BspANI GGCC 3 cut(s) 398, 444, 684
BspCNI CTCAG 4 cut(s) 441, 480, 601, 706
BspFNI CGCG 4 cut(s) 16, 50, 120, 296
BspLI GGNNCC 3 cut(s) 150, 223, 286
BspMI ACCTGC 1 cut(s) 655
BspPI GGATC 5 cut(s) 143, 156, 340, 714, 827
BspQI GCTCTTC 2 cut(s) 320, 548
BspT107I GGYRCC 1 cut(s) 221
BsrI ACTGG 2 cut(s) 67, 685
BssECI CCNNGG 8 cut(s) 144, 165, 228, 327, 369, 399, 844, 918
BssMI GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
BssT1I CCWWGG 3 cut(s) 228, 399, 918
Bst2UI CCWGG 3 cut(s) 167, 388, 846
Bst4CI ACNGT 2 cut(s) 767, 1127
Bst6I CTCTTC 3 cut(s) 320, 477, 548
BstC8I GCNNGC 3 cut(s) 37, 633, 686
BstDEI CTNAG 4 cut(s) 428, 467, 588, 714
BstDSI CCRYGG 2 cut(s) 327, 399
BstF5I GGATG 1 cut(s) 580
BstFNI CGCG 4 cut(s) 16, 50, 120, 296
BstH2I RGCGCY 2 cut(s) 193, 302
BstHHI GCGC 4 cut(s) 18, 122, 192, 301
BstKTI GATC 6 cut(s) 151, 252, 335, 709, 835, 1088
BstMBI GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
BstMWI GCNNNNNNNGC 3 cut(s) 13, 310, 610
BstNI CCWGG 3 cut(s) 167, 388, 846
BstNSI RCATGY 2 cut(s) 750, 865
BstPAI GACNNNNGTC 1 cut(s) 371
BstSCI CCNGG 5 cut(s) 144, 165, 386, 570, 844
BstSFI CTRYAG 1 cut(s) 19
BstUI CGCG 4 cut(s) 16, 50, 120, 296
BstV1I GCAGC 2 cut(s) 288, 1025
BstX2I RGATCY 3 cut(s) 148, 706, 1085
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 3 cut(s) 148, 706, 1085
BsuRI GGCC 3 cut(s) 398, 444, 684
BtgI CCRYGG 2 cut(s) 327, 399
BtgZI GCGATG 1 cut(s) 442
BtrI CACGTC 1 cut(s) 108
BtsCI GGATG 1 cut(s) 580
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 2 cut(s) 678, 708
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 3 cut(s) 37, 633, 686
CfoI GCGC 4 cut(s) 18, 122, 192, 301
Cfr13I GGNCC 1 cut(s) 442
CseI GACGC 1 cut(s) 39
CviAII CATG 7 cut(s) 181, 400, 747, 862, 885, 953, 1073
DdeI CTNAG 4 cut(s) 428, 467, 588, 714
DpnI GATC 6 cut(s) 150, 251, 334, 708, 834, 1087
DpnII GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
DraI TTTAAA 1 cut(s) 879
EaeI YGGCCR 2 cut(s) 396, 682
Eam1104I CTCTTC 3 cut(s) 320, 477, 548
EarI CTCTTC 3 cut(s) 320, 477, 548
EciI GGCGGA 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 3 cut(s) 228, 399, 918
Eco24I GRGCYC 1 cut(s) 468
Eco47III AGCGCT 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 466
EcoRI GAATTC 2 cut(s) 583, 808
EcoRII CCWGG 3 cut(s) 165, 386, 844
EcoT14I CCWWGG 3 cut(s) 228, 399, 918
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 3 cut(s) 228, 399, 918
FaeI CATG 7 cut(s) 184, 403, 750, 865, 888, 956, 1076
FaqI GGGAC 1 cut(s) 37
FatI CATG 7 cut(s) 180, 399, 746, 861, 884, 952, 1072
FauI CCCGC 1 cut(s) 109
FauNDI CATATG 1 cut(s) 1108
Fnu4HI GCNGC 3 cut(s) 297, 302, 1039
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 3 cut(s) 297, 302, 1039
FspBI CTAG 1 cut(s) 605
GlaI GCGC 4 cut(s) 17, 121, 191, 300
GluI GCNGC 3 cut(s) 297, 302, 1039
GsuI CTGGAG 1 cut(s) 836
HaeII RGCGCY 2 cut(s) 193, 302
HaeIII GGCC 3 cut(s) 398, 444, 684
HapII CCGG 2 cut(s) 146, 572
HgaI GACGC 1 cut(s) 39
HhaI GCGC 4 cut(s) 18, 122, 192, 301
Hin1II CATG 7 cut(s) 184, 403, 750, 865, 888, 956, 1076
Hin6I GCGC 4 cut(s) 16, 120, 190, 299
HinP1I GCGC 4 cut(s) 16, 120, 190, 299
HincII GTYRAC 2 cut(s) 366, 1020
HindII GTYRAC 2 cut(s) 366, 1020
HindIII AAGCTT 1 cut(s) 967
HinfI GANTC 3 cut(s) 235, 449, 800
HpaII CCGG 2 cut(s) 146, 572
HphI GGTGA 6 cut(s) 151, 181, 244, 730, 1103, 1115
Hpy166II GTNNAC 3 cut(s) 366, 502, 1020
Hpy188I TCNGA 7 cut(s) 244, 435, 454, 622, 1033, 1051, 1083
Hpy188III TCNNGA 4 cut(s) 319, 596, 815, 854
Hpy8I GTNNAC 3 cut(s) 366, 502, 1020
Hpy99I CGWCG 3 cut(s) 16, 128, 260
HpyAV CCTTC 2 cut(s) 343, 505
HpyCH4III ACNGT 2 cut(s) 767, 1127
HpyCH4IV ACGT 4 cut(s) 107, 392, 785, 984
HpyCH4V TGCA 4 cut(s) 35, 304, 701, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 310, 610
HpyF3I CTNAG 4 cut(s) 428, 467, 588, 714
HpySE526I ACGT 4 cut(s) 107, 392, 785, 984
Hsp92II CATG 7 cut(s) 184, 403, 750, 865, 888, 956, 1076
HspAI GCGC 4 cut(s) 16, 120, 190, 299
Kzo9I GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
LguI GCTCTTC 2 cut(s) 320, 548
LmnI GCTCC 6 cut(s) 29, 44, 197, 290, 622, 693
Lsp1109I GCAGC 2 cut(s) 288, 1025
LweI GCATC 3 cut(s) 22, 465, 1084
MaeI CTAG 1 cut(s) 605
MaeII ACGT 4 cut(s) 107, 392, 785, 984
MaeIII GTNAC 2 cut(s) 667, 938
MalI GATC 6 cut(s) 150, 251, 334, 708, 834, 1087
MboI GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
MboII GAAGA 6 cut(s) 307, 358, 494, 499, 565, 647
MflI RGATCY 3 cut(s) 148, 706, 1085
MhlI GDGCHC 2 cut(s) 468, 560
MlsI TGGCCA 2 cut(s) 398, 684
MluCI AATT 6 cut(s) 506, 583, 808, 923, 1045, 1129
MluNI TGGCCA 2 cut(s) 398, 684
MmeI TCCRAC 4 cut(s) 312, 600, 806, 860
Mox20I TGGCCA 2 cut(s) 398, 684
MroXI GAANNNNTTC 2 cut(s) 176, 314
MscI TGGCCA 2 cut(s) 398, 684
MseI TTAA 4 cut(s) 216, 878, 890, 999
MslI CAYNNNNRTG 2 cut(s) 167, 957
Msp20I TGGCCA 2 cut(s) 398, 684
MspI CCGG 2 cut(s) 146, 572
MspR9I CCNGG 5 cut(s) 146, 167, 388, 572, 846
MvaI CCWGG 3 cut(s) 167, 388, 846
MvnI CGCG 4 cut(s) 16, 50, 120, 296
MwoI GCNNNNNNNGC 3 cut(s) 13, 310, 610
NciI CCSGG 2 cut(s) 146, 572
NcoI CCATGG 1 cut(s) 399
NdeI CATATG 1 cut(s) 1108
NdeII GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
NlaIII CATG 7 cut(s) 184, 403, 750, 865, 888, 956, 1076
NlaIV GGNNCC 3 cut(s) 150, 223, 286
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 2 cut(s) 750, 865
OliI CACNNNNGTG 1 cut(s) 167
PciI ACATGT 1 cut(s) 861
PciSI GCTCTTC 2 cut(s) 320, 548
PcsI WCGNNNNNNNCGW 1 cut(s) 1123
PdmI GAANNNNTTC 2 cut(s) 176, 314
PfeI GAWTC 3 cut(s) 235, 449, 800
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 3 cut(s) 298, 303, 1040
PscI ACATGT 1 cut(s) 861
PshAI GACNNNNGTC 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 165, 386, 844
PspGI CCWGG 3 cut(s) 165, 386, 844
PspN4I GGNNCC 3 cut(s) 150, 223, 286
PspPI GGNCC 1 cut(s) 442
PsuI RGATCY 3 cut(s) 148, 706, 1085
RseI CAYNNNNRTG 2 cut(s) 167, 957
SacI GAGCTC 1 cut(s) 468
SapI GCTCTTC 2 cut(s) 320, 548
SaqAI TTAA 4 cut(s) 216, 878, 890, 999
SatI GCNGC 3 cut(s) 297, 302, 1039
Sau3AI GATC 6 cut(s) 148, 249, 332, 706, 832, 1085
Sau96I GGNCC 1 cut(s) 442
ScrFI CCNGG 5 cut(s) 146, 167, 388, 572, 846
SduI GDGCHC 2 cut(s) 468, 560
SfaNI GCATC 3 cut(s) 22, 465, 1084
SfcI CTRYAG 1 cut(s) 19
SmiI ATTTAAAT 1 cut(s) 879
SmiMI CAYNNNNRTG 2 cut(s) 167, 957
Sse9I AATT 6 cut(s) 506, 583, 808, 923, 1045, 1129
SsiI CCGC 5 cut(s) 48, 102, 225, 296, 338
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 5 cut(s) 144, 165, 386, 570, 844
StyI CCWWGG 3 cut(s) 228, 399, 918
SwaI ATTTAAAT 1 cut(s) 879
TaaI ACNGT 2 cut(s) 767, 1127
TaiI ACGT 4 cut(s) 110, 395, 788, 987
TaqI TCGA 2 cut(s) 480, 1117
TaqII GACCGA 1 cut(s) 383
TasI AATT 6 cut(s) 506, 583, 808, 923, 1045, 1129
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 3 cut(s) 235, 449, 800
Tru1I TTAA 4 cut(s) 216, 878, 890, 999
Tru9I TTAA 4 cut(s) 216, 878, 890, 999
TscAI CASTG 2 cut(s) 685, 708
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 2 cut(s) 301, 1038
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 4 cut(s) 17, 169, 596, 978
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 2 cut(s) 685, 708
XapI RAATTY 4 cut(s) 583, 808, 923, 1045
XceI RCATGY 2 cut(s) 750, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 1106
XmnI GAANNNNTTC 2 cut(s) 176, 314
XspI CTAG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.