Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000948

Physical Location & Seq

Genomic Coordinates Forward (+)

16604854 .. 16606834

Gene Structure 1981 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1083300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGAATGAGCAGATTCATGTGGTGGACTTTGTGATCCTTTGCATCGGGCGATTCAGCGACGTTCCAAACATTCCCGAGTTCCCTCCAAACAAGGGACCAGAAGCATTTCATGGAGAGGTTATACACTCCATGAATTATGCTGACATGGATTACGAAAGCGCAGCTAAATTTGTGGAAGGAAAGCAAGTAACTGTTGTGGGATTTCAGAAATTTGCAATGGACATTGCAATGGAGTGCTCAAACACAAATGGGGTTGCAAATCCATGTACAGTGTTGTACAAGACAGAACACTGGAATCTTCCTGACTATCTTCCATGGGGCTTTCCCATCGCATACTTATACTTCAATCGCTTCTCGGAGCTCTTGGTACACAAGCCCGGTGAAGGCATCCTGCTCAGTCTCCTTGCAACCATTCTTTCACCTGTGAGATGGGGATTTTCGAAATTTGTCGAAAGCTATATAAATAAGAAGCTTGGTTTAGCCAAGTATGGAATGGTACCTAAGCACAGTTTCCTTCAAGAAATCAGTTCTTGTATGATCGCAACAGTACCGGAAAAGTTCTTTGATCGAGTCCGAGAGGGAAGCATCATACTGAAAAAGTCTCCCTGCGGCTTTGGCTTTTGCCAAGAAGGGATTCTGGTTAATGGTGAAAGCTCCCCTGTGAAGTCAGATTTGATCATATTGGCTACTGGGTTTAGGGGTGAGAAAAAGCTCAAGGACATGTTTGTGTCTCCCACCTTTCAGGACTACATCCTCGGTTCGCCAAAGTCCATATTACCCCTTTACAGGGAATGCATACATGGAAGAATTCCACAGCTAGCTGTGATTGGATTCTCCGAGAGTATTTCGAACTTATACACCTCCGAGATGAGATGCAGATGGCTAGCGGAGCTTCTCGGAGGCACATTTAAGCTACCAATCATAAAAGAGATGGAGAAAGATGTGGAAAAATGGGACGCATACGCAATCCGAATCGTCACCACCAGAGACCCACAAGGGTCGCCAGGAGGAGAGCATGGAAGAGATGGAGAAAGATGTGAAAAAATGGGACGCATACGGGAAGCGAAGCGATACACTTCCAGCCAATACTACCGGAGATCATGCACCGGTGCCCTCCATCTGTGGTACAATGACCAGTTGTGCAAGGATATGGGATGGAACCCCAAAAGAAAGAAGGGACTCTTTGCTGAATTGTTTGAGCCTTATGGCCCCATGGATTATGCTTCCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004497 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009626 GO:0009627 GO:0009628 GO:0009814 GO:0009870 GO:0009987 GO:0010204 GO:0012501 GO:0016491 GO:0023052 GO:0031347 GO:0031349 GO:0033554 GO:0034050 GO:0036293 GO:0036294 GO:0042221 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0065007 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0080134 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00982 ko01120 map00982 map01120

Pfam Domains

Protein Families

FMO-like

Protein Analysis

411

Amino Acids

46.53

Weight (kDa)

7.92

Isoelectric Point (pI)

47.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FMO-like	PF00743	3 - 318	1.1e-17	Flavin-binding monooxygenase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000948)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g16430 FvH4_2g16450
malus_domestica	MD00G1083300.v1.1 MD00G1083500.v1.1 MD05G1133000.v1.1 MD16G1203500.v1.1 MD16G1203700.v1.1
prunus_persica	Prupe.8G177000_v2.0.a1 Prupe.8G177100_v2.0.a1
pyrus_communis	pycom05g13010 pycom05g13030 pycom05g13040
rosa_chinensis	RchiOBHm_Chr6g0280221 RchiOBHm_Chr6g0280281 RchiOBHm_Chr6g0280291 RchiOBHm_Chr6g0280311 RchiOBHm_Chr6g0280341
rosa_laevigata	RLG00000013067 RLG00000013073 RLG00000013075 RLG00000013076
rosa_multiflora	Rmu_sc0000757.1_g000002 Rmu_sc0001572.1_g000020 Rmu_sc0001572.1_g000034 Rmu_sc0002826.1_g000002 Rmu_sc0002858.1_g000010 Rmu_sc0002858.1_g000031 Rmu_sc0004211.1_g000005 Rmu_sc0010624.1_g000006
rosa_roxburghii	Rroxscaffold_7G00188150 Rroxscaffold_7G00188190 Rroxscaffold_7G00188220 Rroxscaffold_7G00188230 Rroxscaffold_7G00188240 Rroxscaffold_7G00188270 Rroxscaffold_7G00188290
rosa_rugosa	Rorug06G0130900 Rorug06G0131200 Rorug06G0131200 Rorug06G0131200 Rorug06G0131500
rosa_samantha	Rh6AG241200 Rh6AG241800 Rh6AG241900 Rh6AG242100 Rh6AG242200 Rh6AG242700 Rh6AG242900 Rh6BG244800 Rh6BG245400 Rh6BG245500 Rh6BG245700 Rh6CG246700 Rh6CG247000 Rh6DG237500 Rh6DG238000 Rh6DG238300 Rh6DG238600
rosa_wichuraiana	Rw5G027390 Rw6G021000 Rw6G021020 Rw6G021040 Rw6G021070 Rw6G021120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	496
AccB1I	GGYRCC	2 cut(s)	496, 1109
AciI	CCGC	2 cut(s)	609, 887
AclWI	GGATC	1 cut(s)	28
AcsI	RAATTY	4 cut(s)	167, 209, 443, 807
AfaI	GTAC	6 cut(s)	268, 278, 369, 498, 549, 1127
AfiI	CCNNNNNNNGG	4 cut(s)	92, 383, 786, 787
AflIII	ACRYGT	1 cut(s)	720
AgeI	ACCGGT	1 cut(s)	1106
AgsI	TTSAA	2 cut(s)	346, 518
AjnI	CCWGG	1 cut(s)	1003
AjuI	GAANNNNNNNTTGG	2 cut(s)	618, 650
Alw21I	GWGCWC	2 cut(s)	239, 363
Alw26I	GTCTC	4 cut(s)	404, 606, 735, 981
AlwI	GGATC	1 cut(s)	28
Ama87I	CYCGRG	1 cut(s)	74
AoxI	GGCC	1 cut(s)	1207
ApeKI	GCWGC	1 cut(s)	161
ApoI	RAATTY	4 cut(s)	167, 209, 443, 807
AsiGI	ACCGGT	1 cut(s)	1106
Asp700I	GAANNNNTTC	2 cut(s)	105, 633
Asp718I	GGTACC	1 cut(s)	496
AspLEI	GCGC	1 cut(s)	161
AspS9I	GGNCC	2 cut(s)	95, 1208
AsuC2I	CCSGG	1 cut(s)	378
AsuHPI	GGTGA	5 cut(s)	392, 411, 659, 713, 970
AsuII	TTCGAA	2 cut(s)	440, 848
AsuNHI	GCTAGC	2 cut(s)	817, 883
AvaI	CYCGRG	1 cut(s)	74
AvaII	GGWCC	1 cut(s)	95
BaeGI	GKGCMC	1 cut(s)	1114
BanI	GGYRCC	2 cut(s)	496, 1109
BanII	GRGCYC	1 cut(s)	363
Bbv12I	GWGCWC	2 cut(s)	239, 363
BbvI	GCAGC	1 cut(s)	173
BccI	CCATC	7 cut(s)	335, 423, 873, 925, 1019, 1125, 1149
BciT130I	CCWGG	1 cut(s)	1005
BclI	TGATCA	1 cut(s)	675
BcnI	CCSGG	1 cut(s)	378
BcoDI	GTCTC	4 cut(s)	404, 606, 735, 981
BfaI	CTAG	2 cut(s)	818, 884
BisI	GCNGC	2 cut(s)	162, 610
BlsI	GCNGC	2 cut(s)	163, 611
Bme1390I	CCNGG	2 cut(s)	378, 1005
Bme18I	GGWCC	1 cut(s)	95
BmeT110I	CYCGRG	1 cut(s)	74
BmgT120I	GGNCC	2 cut(s)	95, 1208
BmiI	GGNNCC	5 cut(s)	96, 498, 1111, 1160, 1210
BmrFI	CCNGG	2 cut(s)	378, 1005
BmrI	ACTGGG	1 cut(s)	699
BmsI	GCATC	4 cut(s)	51, 396, 594, 863
BmtI	GCTAGC	2 cut(s)	821, 887
BmuI	ACTGGG	1 cut(s)	699
Bpu10I	CCTNAGC	1 cut(s)	501
Bpu14I	TTCGAA	2 cut(s)	440, 848
BpuEI	CTTGAG	1 cut(s)	698
BpuMI	CCSGG	1 cut(s)	378
BsaI	GGTCTC	1 cut(s)	981
BsaJI	CCNNGG	3 cut(s)	314, 754, 1212
BsaWI	WCCGGW	3 cut(s)	550, 1092, 1106
Bsc4I	CCNNNNNNNGG	4 cut(s)	92, 383, 786, 787
Bse118I	RCCGGY	1 cut(s)	1106
Bse1I	ACTGG	3 cut(s)	296, 694, 1135
Bse3DI	GCAATG	3 cut(s)	222, 222, 234
BseBI	CCWGG	1 cut(s)	1005
BseDI	CCNNGG	3 cut(s)	314, 754, 1212
BseGI	GGATG	3 cut(s)	387, 750, 1160
BseLI	CCNNNNNNNGG	4 cut(s)	92, 383, 786, 787
BseMI	GCAATG	3 cut(s)	222, 222, 234
BseMII	CTCAG	1 cut(s)	409
BseNI	ACTGG	3 cut(s)	296, 694, 1135
BseRI	GAGGAG	1 cut(s)	1023
BseSI	GKGCMC	1 cut(s)	1114
BseXI	GCAGC	1 cut(s)	173
BshFI	GGCC	1 cut(s)	1209
BshNI	GGYRCC	2 cut(s)	496, 1109
BshTI	ACCGGT	1 cut(s)	1106
BsiHKAI	GWGCWC	2 cut(s)	239, 363
BsiHKCI	CYCGRG	1 cut(s)	74
BsiSI	CCGG	4 cut(s)	378, 551, 1093, 1107
BslFI	GGGAC	4 cut(s)	108, 968, 1062, 1191
BslI	CCNNNNNNNGG	4 cut(s)	92, 383, 786, 787
BsmAI	GTCTC	4 cut(s)	404, 606, 735, 981
BsmFI	GGGAC	4 cut(s)	108, 968, 1062, 1191
BsmI	GAATGC	1 cut(s)	797
BsnI	GGCC	1 cut(s)	1209
Bso31I	GGTCTC	1 cut(s)	981
BsoBI	CYCGRG	1 cut(s)	74
Bsp119I	TTCGAA	2 cut(s)	440, 848
Bsp1286I	GDGCHC	3 cut(s)	239, 363, 1114
Bsp1407I	TGTACA	2 cut(s)	266, 276
Bsp143I	GATC	5 cut(s)	33, 537, 565, 675, 1097
Bsp19I	CCATGG	2 cut(s)	314, 1212
BspACI	CCGC	2 cut(s)	609, 887
BspANI	GGCC	1 cut(s)	1209
BspCNI	CTCAG	1 cut(s)	408
BspLI	GGNNCC	5 cut(s)	96, 498, 1111, 1160, 1210
BspOI	GCTAGC	2 cut(s)	821, 887
BspPI	GGATC	1 cut(s)	28
BspT104I	TTCGAA	2 cut(s)	440, 848
BspT107I	GGYRCC	2 cut(s)	496, 1109
BspTNI	GGTCTC	1 cut(s)	981
BsrDI	GCAATG	3 cut(s)	222, 222, 234
BsrFI	RCCGGY	1 cut(s)	1106
BsrGI	TGTACA	2 cut(s)	266, 276
BsrI	ACTGG	3 cut(s)	296, 694, 1135
BssAI	RCCGGY	1 cut(s)	1106
BssECI	CCNNGG	3 cut(s)	314, 754, 1212
BssMI	GATC	5 cut(s)	33, 537, 565, 675, 1097
BssT1I	CCWWGG	2 cut(s)	314, 1212
Bst2UI	CCWGG	1 cut(s)	1005
Bst4CI	ACNGT	4 cut(s)	193, 271, 509, 547
Bst6I	CTCTTC	1 cut(s)	1015
BstAUI	TGTACA	2 cut(s)	266, 276
BstBI	TTCGAA	2 cut(s)	440, 848
BstC8I	GCNNGC	2 cut(s)	819, 885
BstDEI	CTNAG	2 cut(s)	395, 501
BstDSI	CCRYGG	2 cut(s)	314, 1212
BstF5I	GGATG	3 cut(s)	387, 750, 1160
BstHHI	GCGC	1 cut(s)	161
BstKTI	GATC	5 cut(s)	36, 540, 568, 678, 1100
BstMAI	GTCTC	4 cut(s)	404, 606, 735, 981
BstMBI	GATC	5 cut(s)	33, 537, 565, 675, 1097
BstMWI	GCNNNNNNNGC	2 cut(s)	615, 889
BstNI	CCWGG	1 cut(s)	1005
BstNSI	RCATGY	1 cut(s)	724
BstSCI	CCNGG	2 cut(s)	376, 1003
BstSLI	GKGCMC	1 cut(s)	1114
BstV1I	GCAGC	1 cut(s)	173
BsuRI	GGCC	1 cut(s)	1209
BtgI	CCRYGG	2 cut(s)	314, 1212
BtgZI	GCGATG	1 cut(s)	313
BtsCI	GGATG	3 cut(s)	387, 750, 1160
BtsIMutI	CAGTG	2 cut(s)	276, 289
Cac8I	GCNNGC	2 cut(s)	819, 885
CfoI	GCGC	1 cut(s)	161
Cfr10I	RCCGGY	1 cut(s)	1106
Cfr13I	GGNCC	2 cut(s)	95, 1208
CseI	GACGC	2 cut(s)	965, 1059
Csp6I	GTAC	6 cut(s)	267, 277, 368, 497, 548, 1126
CspAI	ACCGGT	1 cut(s)	1106
CviQI	GTAC	6 cut(s)	267, 277, 368, 497, 548, 1126
DdeI	CTNAG	2 cut(s)	395, 501
DpnI	GATC	5 cut(s)	35, 539, 567, 677, 1099
DpnII	GATC	5 cut(s)	33, 537, 565, 675, 1097
Eam1104I	CTCTTC	1 cut(s)	1015
EarI	CTCTTC	1 cut(s)	1015
Ecl136II	GAGCTC	1 cut(s)	361
Eco130I	CCWWGG	2 cut(s)	314, 1212
Eco24I	GRGCYC	1 cut(s)	363
Eco31I	GGTCTC	1 cut(s)	981
Eco47I	GGWCC	1 cut(s)	95
Eco53kI	GAGCTC	1 cut(s)	361
Eco88I	CYCGRG	1 cut(s)	74
EcoICRI	GAGCTC	1 cut(s)	361
EcoRI	GAATTC	1 cut(s)	807
EcoRII	CCWGG	1 cut(s)	1003
EcoT14I	CCWWGG	2 cut(s)	314, 1212
EcoT22I	ATGCAT	1 cut(s)	797
EcoT38I	GRGCYC	1 cut(s)	363
ErhI	CCWWGG	2 cut(s)	314, 1212
FalI	AAGNNNNNCTT	2 cut(s)	1166, 1198
FaqI	GGGAC	4 cut(s)	108, 968, 1062, 1191
FbaI	TGATCA	1 cut(s)	675
Fnu4HI	GCNGC	2 cut(s)	162, 610
FokI	GGATG	3 cut(s)	374, 737, 1167
FriOI	GRGCYC	1 cut(s)	363
Fsp4HI	GCNGC	2 cut(s)	162, 610
FspBI	CTAG	2 cut(s)	818, 884
GlaI	GCGC	1 cut(s)	160
GluI	GCNGC	2 cut(s)	162, 610
HaeIII	GGCC	1 cut(s)	1209
HapII	CCGG	4 cut(s)	378, 551, 1093, 1107
HgaI	GACGC	2 cut(s)	965, 1059
HhaI	GCGC	1 cut(s)	161
Hin6I	GCGC	1 cut(s)	159
HinP1I	GCGC	1 cut(s)	159
HindIII	AAGCTT	1 cut(s)	470
HinfI	GANTC	8 cut(s)	13, 51, 295, 570, 634, 831, 972, 1179
HpaII	CCGG	4 cut(s)	378, 551, 1093, 1107
HphI	GGTGA	5 cut(s)	392, 411, 659, 713, 970
Hpy166II	GTNNAC	2 cut(s)	25, 370
Hpy188I	TCNGA	8 cut(s)	207, 358, 575, 670, 838, 865, 899, 971
Hpy188III	TCNNGA	5 cut(s)	74, 302, 518, 743, 1230
Hpy8I	GTNNAC	2 cut(s)	25, 370
Hpy99I	CGWCG	1 cut(s)	62
HpyAV	CCTTC	5 cut(s)	170, 377, 524, 623, 1168
HpyCH4III	ACNGT	4 cut(s)	193, 271, 509, 547
HpyCH4IV	ACGT	1 cut(s)	60
HpyCH4V	TGCA	9 cut(s)	42, 215, 227, 257, 407, 795, 876, 1104, 1143
HpyF10VI	GCNNNNNNNGC	2 cut(s)	615, 889
HpyF3I	CTNAG	2 cut(s)	395, 501
HpySE526I	ACGT	1 cut(s)	60
HspAI	GCGC	1 cut(s)	159
KpnI	GGTACC	1 cut(s)	500
Ksp22I	TGATCA	1 cut(s)	675
Kzo9I	GATC	5 cut(s)	33, 537, 565, 675, 1097
LmnI	GCTCC	3 cut(s)	358, 659, 889
Lsp1109I	GCAGC	1 cut(s)	173
LweI	GCATC	4 cut(s)	51, 396, 594, 863
MaeI	CTAG	2 cut(s)	818, 884
MaeII	ACGT	1 cut(s)	60
MaeIII	GTNAC	2 cut(s)	187, 976
MalI	GATC	5 cut(s)	35, 539, 567, 677, 1099
MboI	GATC	5 cut(s)	33, 537, 565, 675, 1097
MboII	GAAGA	4 cut(s)	290, 302, 816, 1032
MhlI	GDGCHC	3 cut(s)	239, 363, 1114
MluCI	AATT	6 cut(s)	133, 167, 209, 443, 807, 1190
MlyI	GAGTC	2 cut(s)	579, 1173
MnlI	CCTC	8 cut(s)	93, 109, 571, 764, 871, 893, 1001, 1124
Mph1103I	ATGCAT	1 cut(s)	797
MroXI	GAANNNNTTC	2 cut(s)	105, 633
MseI	TTAA	2 cut(s)	642, 909
MslI	CAYNNNNRTG	2 cut(s)	227, 725
MspI	CCGG	4 cut(s)	378, 551, 1093, 1107
MspR9I	CCNGG	2 cut(s)	378, 1005
Mva1269I	GAATGC	1 cut(s)	797
MvaI	CCWGG	1 cut(s)	1005
MwoI	GCNNNNNNNGC	2 cut(s)	615, 889
NciI	CCSGG	1 cut(s)	378
NcoI	CCATGG	2 cut(s)	314, 1212
NdeII	GATC	5 cut(s)	33, 537, 565, 675, 1097
NheI	GCTAGC	2 cut(s)	817, 883
NlaIV	GGNNCC	5 cut(s)	96, 498, 1111, 1160, 1210
NmuCI	GTSAC	1 cut(s)	976
NsiI	ATGCAT	1 cut(s)	797
NspI	RCATGY	1 cut(s)	724
NspV	TTCGAA	2 cut(s)	440, 848
PciI	ACATGT	1 cut(s)	720
PctI	GAATGC	1 cut(s)	797
PdmI	GAANNNNTTC	2 cut(s)	105, 633
PfeI	GAWTC	6 cut(s)	13, 51, 295, 634, 831, 972
PinAI	ACCGGT	1 cut(s)	1106
PkrI	GCNGC	2 cut(s)	163, 611
PleI	GAGTC	2 cut(s)	578, 1173
PpsI	GAGTC	2 cut(s)	578, 1173
PscI	ACATGT	1 cut(s)	720
Psp124BI	GAGCTC	1 cut(s)	363
Psp6I	CCWGG	1 cut(s)	1003
PspGI	CCWGG	1 cut(s)	1003
PspN4I	GGNNCC	5 cut(s)	96, 498, 1111, 1160, 1210
PspPI	GGNCC	2 cut(s)	95, 1208
RsaI	GTAC	6 cut(s)	268, 278, 369, 498, 549, 1127
RsaNI	GTAC	6 cut(s)	267, 277, 368, 497, 548, 1126
RseI	CAYNNNNRTG	2 cut(s)	227, 725
SacI	GAGCTC	1 cut(s)	363
SaqAI	TTAA	2 cut(s)	642, 909
SatI	GCNGC	2 cut(s)	162, 610
Sau3AI	GATC	5 cut(s)	33, 537, 565, 675, 1097
Sau96I	GGNCC	2 cut(s)	95, 1208
SchI	GAGTC	2 cut(s)	579, 1173
ScrFI	CCNGG	2 cut(s)	378, 1005
SduI	GDGCHC	3 cut(s)	239, 363, 1114
SfaNI	GCATC	4 cut(s)	51, 396, 594, 863
SfuI	TTCGAA	2 cut(s)	440, 848
SgrAI	CRCCGGYG	1 cut(s)	1106
SinI	GGWCC	1 cut(s)	95
SmiMI	CAYNNNNRTG	2 cut(s)	227, 725
SmlI	CTYRAG	1 cut(s)	713
SmoI	CTYRAG	1 cut(s)	713
Sse9I	AATT	6 cut(s)	133, 167, 209, 443, 807, 1190
SsiI	CCGC	2 cut(s)	609, 887
SspMI	CTAG	2 cut(s)	818, 884
SstI	GAGCTC	1 cut(s)	363
StyD4I	CCNGG	2 cut(s)	376, 1003
StyI	CCWWGG	2 cut(s)	314, 1212
TaaI	ACNGT	4 cut(s)	193, 271, 509, 547
TaiI	ACGT	1 cut(s)	63
TaqI	TCGA	4 cut(s)	440, 450, 568, 848
TasI	AATT	6 cut(s)	133, 167, 209, 443, 807, 1190
TatI	WGTACW	2 cut(s)	266, 276
TauI	GCSGC	1 cut(s)	612
TfiI	GAWTC	6 cut(s)	13, 51, 295, 634, 831, 972
Tru1I	TTAA	2 cut(s)	642, 909
Tru9I	TTAA	2 cut(s)	642, 909
TscAI	CASTG	2 cut(s)	276, 296
TseFI	GTSAC	1 cut(s)	976
TseI	GCWGC	1 cut(s)	161
Tsp45I	GTSAC	1 cut(s)	976
TspDTI	ATGAA	4 cut(s)	5, 17, 98, 146
TspRI	CASTG	2 cut(s)	276, 296
VpaK11BI	GGWCC	1 cut(s)	95
XapI	RAATTY	4 cut(s)	167, 209, 443, 807
XceI	RCATGY	1 cut(s)	724
XcmI	CCANNNNNNNNNTGG	1 cut(s)	490
XmnI	GAANNNNTTC	2 cut(s)	105, 633
XspI	CTAG	2 cut(s)	818, 884
Zsp2I	ATGCAT	1 cut(s)	797

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.