Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005368

Physical Location & Seq

Genomic Coordinates Forward (+)

19915427 .. 19919247

Gene Structure 3821 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1095300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGTTCAACTCACTGAGTCAACTCGTTCCCTCACTCGCTCCTCTACCGCAATTCACCGCCAACCTCTTCTTGGCCCGAGCCCTCAAACCCACCGCCGCCGCCGCCACTGCCTCCTTCTCGCTCTCCAACTCGGCCGAGTCGCACTCCAGCCGACTCAGCCTCGCGCATTCCCTCCAATCCGAAACCCTAGAAACCCTAGAATGGGCCTCGGTCTGCAAGCAGCTCTCGGCGCTGGCGTCCACAGCCATGGGCTTCTCGACCGCGCAGAAGGCCCGGGTTCCAGTCGGCCGGACCAAGGAGGAGAGCCAGAAGCTTCTCGATCAGACGGCTGCCGCGGTGGATGCCATCGCGGCCGTCGGTTCTCCACCCTCGGATTTCTCGGCCATCGAGAACGTCTCGGATATCGTGAGCTCCGCCGTCTCAGGCAAATTGCTTACCATTAACGAGCTCTGCGCTGTGCGCAGGACGCTGACTGCCGCGAAGGGATTGTTCGAAAAAGTGAAGGGCTTGGATTTGGGTGCAGATTGTACGGACAGGTACTTGCCCCTGATCGAAATACTCAAGGATTGTGATTTCCTAGTAGAGTTAGAGAAAACGATAGGAGTATGTATAGATTGCAAGCTTTCGATAATACTTGACACGGCTAGCGAAGACTTAGAGATCATTAGGTCAGAGAGGAAGAGGAACATGGAAAATCTGGATTCTTTGCTGAAGGAAGTGTCAACCCAGATTTTTAAATCGGGCGGCATTGATAGCCCTTTGGTGACTATGCGGAGGGCTAGGATGTGCGTTGGTGTTAGGGCCACGCACAAACATTTGCTTCCGGGTTGCATAGTTTTGGATGCTAGTAGTTCTGGAGCAACATACTTTGTGGAACCTAAAGAGGCGGTGGAGTTGAACAACATGGAAGTGAGGCTTTCGAATGCTGAGAGAGCTGAGGAAATCGGCATTTTGAGCTTCCTTACATCTGAAATTGCGAAATCGGAAGTACTGATAATGTATTTGTTAGATAAAGTTTTGGAAGTGGATCTTGCTTTTGCTAGAGCTGCTTATGCTCTGTGGATGAATGGGGTATGTCCAATTTTTAGTTCCAAGGACCTGTATTCTGGTGGGGCTGGTTTTCTGGCAGCTGTAGATATAGAAGGGCTGCAACATCCATTGCTCGTTGAGTCATCCTTAAAAAATTTGTCTGATGATTTTGCATCAAGAAATCCACTTTTCTCCAACAACGGAAATGGTGTAAAAATGATTTCTGGAAGTTTATCTGGTCGTACATCTGATTTTCCAGTGCCAATAGATATCAAAATTGGATGTGGAACCAGAGTGGTTGTAATCTCAGGACCTAATACTGGGGGGAAAACAGCTTCCATGAAAACTTTGGGCCTGGCATCTCTCATGTCGAAGGCTGGCATGTATCTGCCTGCTAAAAACCACCCAAGGCTTCCGTGGTTTGATCTTGTTCTGGCTGATATTGGAGATCATCAGTCTCTTGAGCAAAATCTCTCAACTTTTAGTGGGCACATTTCACGGATTCGTAATATATTGGAAGTGGCCTCAAAAGAATCACTTGTCCTCATTGACGAAATTGGCAGTGGAACCGATCCTTCAGAAGGTGTGGCTCTTTCCGCCAGCATCTTGCTATATCTCAAAGATCGTGTTGACTTAGCCGTTGTGACTACTCACTATGCGGATTTAAGTCGCCTGAAAGAAAAGGATAATCAATTTGAAAATGCAGCCATGGAATTTTCTCCAGAAACCTTACAACCTACTTACCGGATCCTGTGGGGAAGTACTGGTGATTCAAATGCATTGAGTATTGCTAAATCCATCGGTTTCAATCAACACGTAATTGAGCATGCACAGAAATGGGTGCAGAGGTTAATGCCTGAAAAGCAACAGGAGCGAAAAGGTTTGCTATATCGGTCGTTAGTAGAGGAAAGAAACAGACTGGAAGCTCAGGCCAAAATGGCTGCATCTCTTCATTCAGATATCATGGACATTTATTGTGAGATTCACGACGAAGCAGAAGATCTTGAGTTGCGCAAGAGAGCTCTTATGGCAAAGGAAACACTACAGGTTCAGCAAGAAGTGCAGGCTGCAAAATCCCAAATGGAGACTGTGCTGAAGGAGTTTGATAACCGACTCAAAATTGCTGCTGCTGATCAATTGAATTTGCTGATTAGGAAATCAGAAGCTGCAATTGCATCTGTCGTTGAAGCTCACTCTCCTGAGGATGATCTGTTGGTTAGTGAGACATCTGCTACGTCATTTACACCCCGATTGGGAGAGCAAGTTTATTTGAAGAGACTGGGAGATAAGATAGCTACTGTAGCTGAAGCACCTGGAGATGATGGGACAGTCCTTGTACAATATGGTAAAATAAAGGTTCGCTTGAAGAAGAGCGATATTAGAGCTATCCCTAGCGCTGATAAGAATGCCACGCCTAGTTCTGTCCCACGTTTGAAGCAGCAGGTTGGGCGGAGTCGTAATGGGGAAACCGAAGGTGGGGAGGTATCCTATGGGCCAGCGGTACAAACGTCAAAGAACACAGTGGATCTCCGTGGCATGAGGGTGGAGGAAGCTTCTTACCTCCTGGACATGGTCCTGTCTGGTAGAGAGTCTCAATCAGTTATCTTTGTCATACATGGAATGGGCACAGGGGTGGTTAAAGAGAGAGCACTTGAGATTTTGAAAAAACATCCACGAGTAGCCAAGTACGAACCGGAGAGCTCAACGAATTACGGTTGTACAGTTGCTTACATCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03430 map03430

Pfam Domains

Protein Families

MutS_V Smr

Protein Analysis

923

Amino Acids

100.31

Weight (kDa)

5.82

Isoelectric Point (pI)

37.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_V	PF00488	443 - 627	3.9e-34	MutS domain V
MSSS	PF20297	762 - 803	2.3e-08	MutS2 and Smr-associated SH3 domain
Smr	PF01713	851 - 919	3.7e-10	Smr domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005368)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65070 AT1G65070 AT1G65070 AT1G65070
fragaria_vesca	FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390
malus_domestica	MD00G1095300.v1.1
prunus_persica	Prupe.4G045200_v2.0.a1
pyrus_communis	pycom10g24790
rosa_chinensis	RchiOBHm_Chr5g0008191
rosa_laevigata	RLG00000031545
rosa_multiflora	Rmu_co8487121.1_g000001 Rmu_sc0001352.1_g000007 Rmu_sc0001352.1_g000008 Rmu_sc0001352.1_g000029 Rmu_sc0001626.1_g000010
rosa_roxburghii	Rroxscaffold_1G00068290
rosa_rugosa	Rorug04G0434000 Rorug04G0434100
rosa_samantha	Rh5AG062400 Rh5BG058700 Rh5CG069200 Rh5DG058100
rosa_wichuraiana	Rw5G005850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	461, 2042
Acc36I	ACCTGC	1 cut(s)	2461
AccII	CGCG	5 cut(s)	164, 263, 335, 350, 479
AclWI	GGATC	5 cut(s)	1035, 1595, 1771, 1784, 2562
AcoI	YGGCCR	4 cut(s)	132, 286, 351, 381
AcsI	RAATTY	3 cut(s)	1183, 1742, 2170
AcuI	CTGAAG	4 cut(s)	731, 1590, 2144, 2355
AcyI	GRCGYC	1 cut(s)	236
AfaI	GTAC	9 cut(s)	529, 539, 990, 1273, 1792, 2367, 2532, 2717, 2749
AfeI	AGCGCT	1 cut(s)	2425
AfiI	CCNNNNNNNGG	9 cut(s)	70, 201, 202, 295, 1349, 1610, 2282, 2505, 2599
AflIII	ACRYGT	1 cut(s)	1843
AjnI	CCWGG	3 cut(s)	1383, 2341, 2592
AjuI	GAANNNNNNNTTGG	2 cut(s)	1072, 1104
AleI	CACNNNNGTG	1 cut(s)	2660
Alw21I	GWGCWC	5 cut(s)	413, 450, 2053, 2680, 2732
Alw26I	GTCTC	7 cut(s)	400, 424, 1491, 2108, 2246, 2299, 2625
AlwI	GGATC	5 cut(s)	1035, 1595, 1771, 1784, 2562
AlwNI	CAGNNNCTG	1 cut(s)	2195
Ama87I	CYCGRG	2 cut(s)	75, 273
Aor51HI	AGCGCT	1 cut(s)	2425
ApoI	RAATTY	3 cut(s)	1183, 1742, 2170
AspLEI	GCGC	7 cut(s)	166, 232, 265, 455, 462, 2043, 2426
AsuC2I	CCSGG	3 cut(s)	274, 275, 825
AsuHPI	GGTGA	3 cut(s)	46, 775, 1808
AsuII	TTCGAA	2 cut(s)	492, 920
AsuNHI	GCTAGC	1 cut(s)	644
AvaI	CYCGRG	2 cut(s)	75, 273
AvaII	GGWCC	4 cut(s)	291, 1096, 1340, 2602
AxyI	CCTNAGG	1 cut(s)	2229
BaeGI	GKGCMC	2 cut(s)	1521, 2657
BaeI	ACNNNNGTAYC	2 cut(s)	2522, 2555
BamHI	GGATCC	1 cut(s)	1776
BanII	GRGCYC	5 cut(s)	82, 413, 450, 2053, 2732
BauI	CACGAG	1 cut(s)	2703
BbsI	GAAGAC	1 cut(s)	657
Bbv12I	GWGCWC	5 cut(s)	413, 450, 2053, 2680, 2732
BbvCI	CCTCAGC	1 cut(s)	936
BccI	CCATC	4 cut(s)	353, 392, 1835, 2345
BceAI	ACGGC	5 cut(s)	338, 342, 401, 657, 1652
BciT130I	CCWGG	3 cut(s)	1385, 2343, 2594
BciVI	GTATCC	1 cut(s)	2524
BclI	TGATCA	1 cut(s)	2161
BcnI	CCSGG	3 cut(s)	274, 275, 825
BcoDI	GTCTC	7 cut(s)	400, 424, 1491, 2108, 2246, 2299, 2625
BfaI	CTAG	9 cut(s)	188, 197, 578, 645, 780, 846, 1041, 2421, 2445
BfmI	CTRYAG	3 cut(s)	1131, 2072, 2328
BfoI	RGCGCY	2 cut(s)	233, 2427
BfuAI	ACCTGC	1 cut(s)	2461
BfuI	GTATCC	1 cut(s)	2524
BglI	GCCNNNNNGGC	2 cut(s)	350, 1967
BglII	AGATCT	1 cut(s)	2029
BmcAI	AGTACT	2 cut(s)	990, 1792
Bme1390I	CCNGG	6 cut(s)	274, 275, 825, 1385, 2343, 2594
Bme18I	GGWCC	4 cut(s)	291, 1096, 1340, 2602
BmeT110I	CYCGRG	2 cut(s)	75, 273
BmiI	GGNNCC	5 cut(s)	279, 876, 1318, 1597, 1778
BmrFI	CCNGG	6 cut(s)	274, 275, 825, 1385, 2343, 2594
BmrI	ACTGGG	2 cut(s)	1359, 2318
BmsI	GCATC	7 cut(s)	331, 832, 1211, 1397, 1641, 1982, 2213
BmtI	GCTAGC	1 cut(s)	648
BmuI	ACTGGG	2 cut(s)	1359, 2318
BpiI	GAAGAC	1 cut(s)	657
BplI	GAGNNNNNCTC	4 cut(s)	128, 160, 380, 412
BpmI	CTGGAG	4 cut(s)	130, 876, 1734, 2364
Bpu10I	CCTNAGC	2 cut(s)	936, 1956
Bpu14I	TTCGAA	2 cut(s)	492, 920
BpuEI	CTTGAG	4 cut(s)	545, 1511, 2054, 2702
BpuMI	CCSGG	3 cut(s)	274, 275, 825
BsaAI	YACGTR	1 cut(s)	1846
BsaHI	GRCGYC	1 cut(s)	236
BsaWI	WCCGGW	2 cut(s)	1773, 2722
BsaXI	ACNNNNNCTCC	2 cut(s)	2277, 2307
Bsc4I	CCNNNNNNNGG	9 cut(s)	70, 201, 202, 295, 1349, 1610, 2282, 2505, 2599
Bse1I	ACTGG	6 cut(s)	281, 1286, 1354, 1798, 1953, 2313
Bse21I	CCTNAGG	1 cut(s)	2229
Bse3DI	GCAATG	1 cut(s)	1157
BseBI	CCWGG	3 cut(s)	1385, 2343, 2594
BseGI	GGATG	9 cut(s)	346, 789, 847, 1068, 1153, 1172, 1316, 2239, 2698
BseLI	CCNNNNNNNGG	9 cut(s)	70, 201, 202, 295, 1349, 1610, 2282, 2505, 2599
BseMI	GCAATG	1 cut(s)	1157
BseMII	CTCAG	8 cut(s)	5, 169, 435, 918, 927, 1350, 1970, 2220
BseNI	ACTGG	6 cut(s)	281, 1286, 1354, 1798, 1953, 2313
BseRI	GAGGAG	2 cut(s)	30, 314
BseSI	GKGCMC	2 cut(s)	1521, 2657
BseX3I	CGGCCG	3 cut(s)	132, 286, 351
BsgI	GTGCAG	3 cut(s)	540, 1892, 2111
Bsh1236I	CGCG	5 cut(s)	164, 263, 335, 350, 479
Bsh1285I	CGRYCG	5 cut(s)	135, 261, 289, 354, 1925
BsiEI	CGRYCG	5 cut(s)	135, 261, 289, 354, 1925
BsiHKAI	GWGCWC	5 cut(s)	413, 450, 2053, 2680, 2732
BsiHKCI	CYCGRG	2 cut(s)	75, 273
BsiSI	CCGG	5 cut(s)	274, 289, 824, 1774, 2723
BslFI	GGGAC	2 cut(s)	2368, 2438
BslI	CCNNNNNNNGG	9 cut(s)	70, 201, 202, 295, 1349, 1610, 2282, 2505, 2599
BsmAI	GTCTC	7 cut(s)	400, 424, 1491, 2108, 2246, 2299, 2625
BsmBI	CGTCTC	2 cut(s)	400, 424
BsmFI	GGGAC	2 cut(s)	2368, 2438
BsmI	GAATGC	3 cut(s)	166, 928, 2440
BsoBI	CYCGRG	2 cut(s)	75, 273
Bsp119I	TTCGAA	2 cut(s)	492, 920
Bsp1286I	GDGCHC	8 cut(s)	82, 413, 450, 1521, 2053, 2657, 2680, 2732
Bsp1407I	TGTACA	2 cut(s)	2365, 2747
Bsp19I	CCATGG	2 cut(s)	246, 1737
BspCNI	CTCAG	8 cut(s)	6, 168, 434, 919, 928, 1349, 1969, 2221
BspFNI	CGCG	5 cut(s)	164, 263, 335, 350, 479
BspLI	GGNNCC	5 cut(s)	279, 876, 1318, 1597, 1778
BspMI	ACCTGC	1 cut(s)	2461
BspOI	GCTAGC	1 cut(s)	648
BspPI	GGATC	5 cut(s)	1035, 1595, 1771, 1784, 2562
BspQI	GCTCTTC	1 cut(s)	2393
BspT104I	TTCGAA	2 cut(s)	492, 920
BsrDI	GCAATG	1 cut(s)	1157
BsrGI	TGTACA	2 cut(s)	2365, 2747
BsrI	ACTGG	6 cut(s)	281, 1286, 1354, 1798, 1953, 2313
BssNI	GRCGYC	1 cut(s)	236
BssSI	CACGAG	1 cut(s)	2703
BssT1I	CCWWGG	5 cut(s)	246, 294, 1092, 1436, 1737
Bst2BI	CACGAG	1 cut(s)	2703
Bst2UI	CCWGG	3 cut(s)	1385, 2343, 2594
Bst4CI	ACNGT	6 cut(s)	2119, 2329, 2359, 2551, 2744, 2752
Bst6I	CTCTTC	5 cut(s)	71, 674, 1983, 2297, 2393
BstACI	GRCGYC	1 cut(s)	236
BstAPI	GCANNNNNTGC	1 cut(s)	2089
BstAUI	TGTACA	2 cut(s)	2365, 2747
BstBAI	YACGTR	1 cut(s)	1846
BstBI	TTCGAA	2 cut(s)	492, 920
BstDSI	CCRYGG	5 cut(s)	246, 333, 1445, 1737, 2560
BstENI	CCTNNNNNAGG	1 cut(s)	1608
BstF5I	GGATG	9 cut(s)	346, 789, 847, 1068, 1153, 1172, 1316, 2239, 2698
BstFNI	CGCG	5 cut(s)	164, 263, 335, 350, 479
BstH2I	RGCGCY	2 cut(s)	233, 2427
BstHHI	GCGC	7 cut(s)	166, 232, 265, 455, 462, 2043, 2426
BstMAI	GTCTC	7 cut(s)	400, 424, 1491, 2108, 2246, 2299, 2625
BstMCI	CGRYCG	5 cut(s)	135, 261, 289, 354, 1925
BstNI	CCWGG	3 cut(s)	1385, 2343, 2594
BstNSI	RCATGY	2 cut(s)	1414, 1859
BstSCI	CCNGG	6 cut(s)	272, 273, 823, 1383, 2341, 2592
BstSFI	CTRYAG	3 cut(s)	1131, 2072, 2328
BstSLI	GKGCMC	2 cut(s)	1521, 2657
BstUI	CGCG	5 cut(s)	164, 263, 335, 350, 479
BstV2I	GAAGAC	1 cut(s)	657
BstX2I	RGATCY	4 cut(s)	1027, 1776, 2029, 2554
BstXI	CCANNNNNNTGG	1 cut(s)	247
BstYI	RGATCY	4 cut(s)	1027, 1776, 2029, 2554
BstZI	CGGCCG	3 cut(s)	132, 286, 351
Bsu36I	CCTNAGG	1 cut(s)	2229
BsuI	GTATCC	1 cut(s)	2524
BtgI	CCRYGG	5 cut(s)	246, 333, 1445, 1737, 2560
BtgZI	GCGATG	1 cut(s)	331
BtsCI	GGATG	9 cut(s)	346, 789, 847, 1068, 1153, 1172, 1316, 2239, 2698
BtsI	GCAGTG	2 cut(s)	105, 1597
BtsIMutI	CAGTG	5 cut(s)	11, 105, 1293, 1597, 2556
BveI	ACCTGC	1 cut(s)	2461
CaiI	CAGNNNCTG	1 cut(s)	2195
CfoI	GCGC	7 cut(s)	166, 232, 265, 455, 462, 2043, 2426
Cfr42I	CCGCGG	1 cut(s)	336
Cfr9I	CCCGGG	1 cut(s)	273
CseI	GACGC	2 cut(s)	225, 475
Csp6I	GTAC	9 cut(s)	528, 538, 989, 1272, 1791, 2366, 2531, 2716, 2748
CviQI	GTAC	9 cut(s)	528, 538, 989, 1272, 1791, 2366, 2531, 2716, 2748
DraI	TTTAAA	1 cut(s)	736
EaeI	YGGCCR	4 cut(s)	132, 286, 351, 381
EagI	CGGCCG	3 cut(s)	132, 286, 351
Eam1104I	CTCTTC	5 cut(s)	71, 674, 1983, 2297, 2393
EarI	CTCTTC	5 cut(s)	71, 674, 1983, 2297, 2393
EciI	GGCGGA	3 cut(s)	403, 1615, 2494
Ecl136II	GAGCTC	4 cut(s)	411, 448, 2051, 2730
EclXI	CGGCCG	3 cut(s)	132, 286, 351
Eco130I	CCWWGG	5 cut(s)	246, 294, 1092, 1436, 1737
Eco24I	GRGCYC	5 cut(s)	82, 413, 450, 2053, 2732
Eco32I	GATATC	3 cut(s)	403, 1300, 1990
Eco47I	GGWCC	4 cut(s)	291, 1096, 1340, 2602
Eco47III	AGCGCT	1 cut(s)	2425
Eco52I	CGGCCG	3 cut(s)	132, 286, 351
Eco53kI	GAGCTC	4 cut(s)	411, 448, 2051, 2730
Eco57I	CTGAAG	4 cut(s)	731, 1590, 2144, 2355
Eco81I	CCTNAGG	1 cut(s)	2229
Eco88I	CYCGRG	2 cut(s)	75, 273
EcoICRI	GAGCTC	4 cut(s)	411, 448, 2051, 2730
EcoNI	CCTNNNNNAGG	1 cut(s)	1608
EcoO109I	RGGNCCY	2 cut(s)	1096, 1340
EcoRII	CCWGG	3 cut(s)	1383, 2341, 2592
EcoRV	GATATC	3 cut(s)	403, 1300, 1990
EcoT14I	CCWWGG	5 cut(s)	246, 294, 1092, 1436, 1737
EcoT22I	ATGCAT	1 cut(s)	1810
EcoT38I	GRGCYC	5 cut(s)	82, 413, 450, 2053, 2732
ErhI	CCWWGG	5 cut(s)	246, 294, 1092, 1436, 1737
Esp3I	CGTCTC	2 cut(s)	400, 424
FalI	AAGNNNNNCTT	8 cut(s)	900, 932, 1014, 1046, 1551, 1583, 2375, 2407
FaqI	GGGAC	2 cut(s)	2368, 2438
FbaI	TGATCA	1 cut(s)	2161
FokI	GGATG	9 cut(s)	353, 796, 854, 1075, 1140, 1159, 1323, 2246, 2685
FriOI	GRGCYC	5 cut(s)	82, 413, 450, 2053, 2732
FspBI	CTAG	9 cut(s)	188, 197, 578, 645, 780, 846, 1041, 2421, 2445
FspI	TGCGCA	2 cut(s)	461, 2042
GlaI	GCGC	7 cut(s)	165, 231, 264, 454, 461, 2042, 2425
GsuI	CTGGAG	4 cut(s)	130, 876, 1734, 2364
HaeII	RGCGCY	2 cut(s)	233, 2427
HapII	CCGG	5 cut(s)	274, 289, 824, 1774, 2723
HgaI	GACGC	2 cut(s)	225, 475
HhaI	GCGC	7 cut(s)	166, 232, 265, 455, 462, 2043, 2426
Hin1I	GRCGYC	1 cut(s)	236
Hin6I	GCGC	7 cut(s)	164, 230, 263, 453, 460, 2041, 2424
HinP1I	GCGC	7 cut(s)	164, 230, 263, 453, 460, 2041, 2424
HincII	GTYRAC	3 cut(s)	20, 723, 1660
HindII	GTYRAC	3 cut(s)	20, 723, 1660
HindIII	AAGCTT	3 cut(s)	311, 620, 2580
HpaII	CCGG	5 cut(s)	274, 289, 824, 1774, 2723
HphI	GGTGA	3 cut(s)	46, 775, 1808
Hpy166II	GTNNAC	4 cut(s)	20, 240, 723, 1660
Hpy8I	GTNNAC	4 cut(s)	20, 240, 723, 1660
Hpy99I	CGWCG	2 cut(s)	359, 2021
HpyCH4III	ACNGT	6 cut(s)	2119, 2329, 2359, 2551, 2744, 2752
HpyCH4IV	ACGT	5 cut(s)	393, 1845, 2264, 2458, 2537
HpySE526I	ACGT	5 cut(s)	393, 1845, 2264, 2458, 2537
Hsp92I	GRCGYC	1 cut(s)	236
HspAI	GCGC	7 cut(s)	164, 230, 263, 453, 460, 2041, 2424
Ksp22I	TGATCA	1 cut(s)	2161
KspI	CCGCGG	1 cut(s)	336
LguI	GCTCTTC	1 cut(s)	2393
LmnI	GCTCC	4 cut(s)	43, 416, 857, 1900
LweI	GCATC	7 cut(s)	331, 832, 1211, 1397, 1641, 1982, 2213
MaeI	CTAG	9 cut(s)	188, 197, 578, 645, 780, 846, 1041, 2421, 2445
MaeII	ACGT	5 cut(s)	393, 1845, 2264, 2458, 2537
MaeIII	GTNAC	2 cut(s)	763, 1672
MboII	GAAGA	8 cut(s)	58, 662, 691, 1970, 2039, 2314, 2407, 2410
MfeI	CAATTG	2 cut(s)	2165, 2199
MflI	RGATCY	4 cut(s)	1027, 1776, 2029, 2554
MhlI	GDGCHC	8 cut(s)	82, 413, 450, 1521, 2053, 2657, 2680, 2732
MlyI	GAGTC	7 cut(s)	25, 146, 147, 1178, 2136, 2491, 2627
MmeI	TCCRAC	2 cut(s)	150, 1248
Mph1103I	ATGCAT	1 cut(s)	1810
MseI	TTAA	6 cut(s)	441, 735, 1178, 1694, 1880, 2667
MslI	CAYNNNNRTG	5 cut(s)	245, 908, 1864, 2570, 2660
MspA1I	CMGCKG	3 cut(s)	335, 1130, 2528
MspI	CCGG	5 cut(s)	274, 289, 824, 1774, 2723
MspR9I	CCNGG	6 cut(s)	274, 275, 825, 1385, 2343, 2594
MunI	CAATTG	2 cut(s)	2165, 2199
Mva1269I	GAATGC	3 cut(s)	166, 928, 2440
MvaI	CCWGG	3 cut(s)	1385, 2343, 2594
MvnI	CGCG	5 cut(s)	164, 263, 335, 350, 479
NciI	CCSGG	3 cut(s)	274, 275, 825
NcoI	CCATGG	2 cut(s)	246, 1737
NheI	GCTAGC	1 cut(s)	644
NlaIV	GGNNCC	5 cut(s)	279, 876, 1318, 1597, 1778
NmeAIII	GCCGAG	4 cut(s)	110, 160, 206, 359
NmuCI	GTSAC	2 cut(s)	763, 1672
NsbI	TGCGCA	2 cut(s)	461, 2042
NsiI	ATGCAT	1 cut(s)	1810
NspI	RCATGY	2 cut(s)	1414, 1859
NspV	TTCGAA	2 cut(s)	492, 920
OliI	CACNNNNGTG	1 cut(s)	2660
PaeI	GCATGC	1 cut(s)	1859
PciSI	GCTCTTC	1 cut(s)	2393
PcsI	WCGNNNNNNNCGW	1 cut(s)	233
PctI	GAATGC	3 cut(s)	166, 928, 2440
PfeI	GAWTC	5 cut(s)	701, 1531, 1562, 1799, 2011
PflFI	GACNNNGTC	1 cut(s)	2600
PfoI	TCCNGGA	1 cut(s)	2592
PleI	GAGTC	7 cut(s)	24, 145, 147, 1177, 2136, 2490, 2626
PpsI	GAGTC	7 cut(s)	24, 145, 147, 1177, 2136, 2490, 2626
Ppu21I	YACGTR	1 cut(s)	1846
PpuMI	RGGWCCY	2 cut(s)	1096, 1340
Psp124BI	GAGCTC	4 cut(s)	413, 450, 2053, 2732
Psp5II	RGGWCCY	2 cut(s)	1096, 1340
Psp6I	CCWGG	3 cut(s)	1383, 2341, 2592
PspGI	CCWGG	3 cut(s)	1383, 2341, 2592
PspN4I	GGNNCC	5 cut(s)	279, 876, 1318, 1597, 1778
PspPPI	RGGWCCY	2 cut(s)	1096, 1340
PstNI	CAGNNNCTG	1 cut(s)	2195
PsuI	RGATCY	4 cut(s)	1027, 1776, 2029, 2554
PsyI	GACNNNGTC	1 cut(s)	2600
PvuII	CAGCTG	1 cut(s)	1130
RsaI	GTAC	9 cut(s)	529, 539, 990, 1273, 1792, 2367, 2532, 2717, 2749
RsaNI	GTAC	9 cut(s)	528, 538, 989, 1272, 1791, 2366, 2531, 2716, 2748
RseI	CAYNNNNRTG	5 cut(s)	245, 908, 1864, 2570, 2660
SacI	GAGCTC	4 cut(s)	413, 450, 2053, 2732
SacII	CCGCGG	1 cut(s)	336
SapI	GCTCTTC	1 cut(s)	2393
SaqAI	TTAA	6 cut(s)	441, 735, 1178, 1694, 1880, 2667
ScaI	AGTACT	2 cut(s)	990, 1792
SchI	GAGTC	7 cut(s)	25, 146, 147, 1178, 2136, 2491, 2627
ScrFI	CCNGG	6 cut(s)	274, 275, 825, 1385, 2343, 2594
SduI	GDGCHC	8 cut(s)	82, 413, 450, 1521, 2053, 2657, 2680, 2732
SfaNI	GCATC	7 cut(s)	331, 832, 1211, 1397, 1641, 1982, 2213
SfcI	CTRYAG	3 cut(s)	1131, 2072, 2328
Sfr303I	CCGCGG	1 cut(s)	336
SfuI	TTCGAA	2 cut(s)	492, 920
SgrBI	CCGCGG	1 cut(s)	336
SinI	GGWCC	4 cut(s)	291, 1096, 1340, 2602
SmaI	CCCGGG	1 cut(s)	275
SmiMI	CAYNNNNRTG	5 cut(s)	245, 908, 1864, 2570, 2660
SmlI	CTYRAG	4 cut(s)	560, 1490, 2033, 2681
SmoI	CTYRAG	4 cut(s)	560, 1490, 2033, 2681
SphI	GCATGC	1 cut(s)	1859
SspMI	CTAG	9 cut(s)	188, 197, 578, 645, 780, 846, 1041, 2421, 2445
SstI	GAGCTC	4 cut(s)	413, 450, 2053, 2732
StyD4I	CCNGG	6 cut(s)	272, 273, 823, 1383, 2341, 2592
StyI	CCWWGG	5 cut(s)	246, 294, 1092, 1436, 1737
TaaI	ACNGT	6 cut(s)	2119, 2329, 2359, 2551, 2744, 2752
TaiI	ACGT	5 cut(s)	396, 1848, 2267, 2461, 2540
TaqI	TCGA	8 cut(s)	257, 318, 387, 492, 552, 626, 920, 1400
TaqII	GACCGA	2 cut(s)	199, 1911
TatI	WGTACW	4 cut(s)	988, 1790, 2365, 2747
TauI	GCSGC	7 cut(s)	98, 101, 104, 335, 353, 479, 747
TfiI	GAWTC	5 cut(s)	701, 1531, 1562, 1799, 2011
Tru1I	TTAA	6 cut(s)	441, 735, 1178, 1694, 1880, 2667
Tru9I	TTAA	6 cut(s)	441, 735, 1178, 1694, 1880, 2667
TscAI	CASTG	5 cut(s)	18, 112, 1293, 1597, 2556
TseFI	GTSAC	2 cut(s)	763, 1672
Tsp45I	GTSAC	2 cut(s)	763, 1672
TspDTI	ATGAA	3 cut(s)	1079, 1385, 1970
TspGWI	ACGGA	5 cut(s)	545, 1245, 1434, 1543, 2549
TspMI	CCCGGG	1 cut(s)	273
TspRI	CASTG	5 cut(s)	18, 112, 1293, 1597, 2556
Tth111I	GACNNNGTC	1 cut(s)	2600
VpaK11BI	GGWCC	4 cut(s)	291, 1096, 1340, 2602
XagI	CCTNNNNNAGG	1 cut(s)	1608
XapI	RAATTY	3 cut(s)	1183, 1742, 2170
XceI	RCATGY	2 cut(s)	1414, 1859
XcmI	CCANNNNNNNNNTGG	3 cut(s)	67, 1375, 1443
XmaI	CCCGGG	1 cut(s)	273
XspI	CTAG	9 cut(s)	188, 197, 578, 645, 780, 846, 1041, 2421, 2445
ZrmI	AGTACT	2 cut(s)	990, 1792
Zsp2I	ATGCAT	1 cut(s)	1810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.