Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0005368

Physical Location & Seq

Genomic Coordinates Reverse (-)

5263959 .. 5267570

Gene Structure 3612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG062400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGCAAAAACCAATGCTCACCTCCGTCACTCCATTCAACCTAACCTTCTTCCTCTCCTCCCCAAAATCTTCATACCTCTTCCCCAAACCCACCGCCAGTGCCGCCTCCGCCGTCACTAACTTCTCGCTCGCCCATTCCCTCCAATCCGAAACCCTCGAAATCCTCGAATGGCCCTCCGTCTGCAACCACCTCTCCGCTCTCACCTCCACCTCCATGGGCTTCTCCGCCGCGCAGAAGGCCCAAATTCCCTTCGGAAAATCCAAGTCCGAGAGCCAGAAGCTTCTAGATCAAACCGCCGCCGCGGTCTCCGCCATCGCAGCCATCGGTTCTCCGCCGTCCGATTTCCACTCCATCGAGGACGTCTCCGAGATCGTAAACGCTGCCGTTTTGGGCAAATTGCTCACCATCAACGAGCTCTGCGCTGTTCGTAGAACACTCATAGCCGCAAAGGCCTTGTTCGAGAAGCTGAAGGAACTAGCTTCGAAAGCAGAAGCAGATTCTAATAGGTACTTGCCATTGCTTCAAGTACTTGAGGATTGTGATTTTCTAGTGAAGCTGGAGAGAACTATAGGGTTGTGCATAGACTGCAATCTTTCGGAGATTCTCGACACGGCTGGTGAAGACTTGGAGATCATTAGGTTCGAAAAGAAGAGGAACATGGAGAAATTGGATGCTTTGTTGAAGGAAATGTCCTCTAAGATATTTAAGGCTGGTGGGATTGACAGTCCTTTGGTGACGAAGCGCAGGGCGAGGATGTGCGTGGGTGTTAGAGCTAGGTACAAGTATTTGGTTCCTGATGGCGTGGTGCTGGATGCTAGTAGTTCTGGTGCGACGTACTTCATGGAGCCGAGTGAGGTGGTGGAGTTGAATAACATGGAAGTGAGGCTTTCGAATGCTGAGAAGGCTGAGGAAATAGGCATTTTGAGCTTCCTTACATCAGAAATTGCAAAATCAGAAGGGGAGATAGTGTATTTGTTAGATAAGATTGTAGAAGTTGATCTTGCTTTTGCTAGAGCTGTGTATGCTCAGTGGATGAATGGGGTATGCCCAATTTTCAGTTCTAAGGATGGCAAGGATTTAGAGAATGGTGGAGCTGAAATTGGGATATCGATAGATATTGAAGGAATACAACATCCTTTGCTCCTTGAGTCATCTCTTAGTAGTTTGTCTGATGCCCTTGCATCCAGTTCTAGAAGCACACTTTCCTCCAAAGACGGAAATGATGTAAAAATGGTCTATCGAAGTTTATCTAGTGGTGTATCTGATTTTCCAGTGCCAATAGATATCAAAATTGGATATGGAACCAGAGTGGTTGTAATCTCAGGGCCTAATACTGGCGGGAAAACAGCTTCCATGAAAACTTTGGGCCTGGCATCTCTTATGTCGAAAGCTGGAATGTATCTGCCTGCTAAAAGCCAGCCAAAGCTTCCATGGTTTGATCTTGTTCTAGCTGATATTGGAGATCAACAGTCTCTTGAGCAAAGCCTCTCAACATTTAGTGGGCACATATCACGGATCTGTAATATTTTGGAAGTCGCCTCAAAAGAATCACTTGTCCTCATTGATGAAATTGGTAGTGGAACCGATCCTTCAGAAGGTGTGGCTCTTTCTACCAGCATTTTGCAATATCTCAAAGATCGCGTTAACTTAGCTGTTGTGACTACTCACTACGCGGATTTAAGTCTTCTAAAAGAAAAGGATCACCAGTTTGAAAATGCAGCCATGGAGTTTTCTCCAGAAACCTTACAACCAACTTATCGGGTCCTATGGGGAAGTATTGGTGACTCAAATGCATTGAGTATTGCTAAATCAATTGGTTTTAATCAACAAATAATTGAACGCGCACAAGATTGGGTGGAAAGATTAAGGCCAGAAAAGCAGCAAGAGCGTAAAGGTTTGCTATATCAGTCATTAATAGAGGAAAGAAACAGACTGGAAGCTCAGGCTAAGATGGCAGCGTCTCTTCATTCAGAAACCAGGGATATTTATCATGAGATCCAAGATGAGGCAGAAGATCTTGACGTGCGCAAGAGAGCTCTTATGGCAAAGGAAACCCTACAGGTTTGGGAAGAAGTGAAGATTGTCAAATCTCAATTGGAAGCTGTGCTTCAGGAGTTTGATAACCGACTCAAAACTGCTGGTGCTGATCAGTTGAATTTGCTGATAAAGAAATCAGAAGCTGCAATTGCATCTATTATTGAGGCTCACAGTCCTGAAGATGGTTTCTTGGTAAATGAGACATCTGAAACTTCATACACACCTCGATTTGGGGAACAAGTCTATTTGAAGGGACTCAGAGATAAGATAGCTACCGTAGTTGAAGCACCTGGAGATGATGGGACAGTGCTCGTACAATATGGTAAAATTAAGGTTAGGTTGAAGAATAATGAGATCAGAGCTATTCCTAGTAGTGATAAGAATGCCGCGACTAGTTCTGTCCCACGTTTGAAGCAGCGGGTTTGGCGGAGCCGTACTGTGGAAAACAAAGATGCGGAGGTTTCTTATAGTCCAGCGGTACAAACATCAAAGAACACAGTGGATCTTCGTGGCATGAGGGCGGAGGAAGCTTCTTACAATCTGGACATGGCCATCGCTACTAGAGAGTCTCAGTCAGTTCTTTTTGTTGTACATGGGATGGGCACAGGGGTTGTCAAAGAGCGAGCACTTGAGCTTTTAAAGAAACATCCACGAGTAGCCAAGTATGAAGCGGAAAGTCCAATGAATTATGGTTGTACAGTTGCTTACATCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03430 map03430

Pfam Domains

Protein Families

MutS_V Smr

Protein Analysis

917

Amino Acids

100.65

Weight (kDa)

5.49

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_V	PF00488	438 - 622	1.7e-34	MutS domain V
MSSS	PF20297	757 - 798	1.7e-07	MutS2 and Smr-associated SH3 domain
Smr	PF01713	846 - 915	2.9e-10	Smr domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005368)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65070 AT1G65070 AT1G65070 AT1G65070
fragaria_vesca	FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390
malus_domestica	MD00G1095300.v1.1
prunus_persica	Prupe.4G045200_v2.0.a1
pyrus_communis	pycom10g24790
rosa_chinensis	RchiOBHm_Chr5g0008191
rosa_laevigata	RLG00000031545
rosa_multiflora	Rmu_co8487121.1_g000001 Rmu_sc0001352.1_g000007 Rmu_sc0001352.1_g000008 Rmu_sc0001352.1_g000029 Rmu_sc0001626.1_g000010
rosa_roxburghii	Rroxscaffold_1G00068290
rosa_rugosa	Rorug04G0434000 Rorug04G0434100
rosa_samantha	Rh5AG062400 Rh5BG058700 Rh5CG069200 Rh5DG058100
rosa_wichuraiana	Rw5G005850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	363
Acc16I	TGCGCA	1 cut(s)	2027
AccBSI	CCGCTC	1 cut(s)	197
AccII	CGCG	6 cut(s)	230, 302, 1641, 1673, 1842, 2426
AclWI	GGATC	5 cut(s)	1523, 1580, 1707, 1990, 2547
AcoI	YGGCCR	1 cut(s)	2586
AcsI	RAATTY	2 cut(s)	243, 2155
AcuI	CTGAAG	4 cut(s)	488, 1575, 2093, 2235
AcyI	GRCGYC	1 cut(s)	360
AfaI	GTAC	9 cut(s)	509, 528, 779, 836, 2352, 2473, 2517, 2628, 2734
AfiI	CCNNNNNNNGG	6 cut(s)	1334, 1595, 2005, 2219, 2267, 2476
AhlI	ACTAGT	1 cut(s)	2429
AjiI	CACGTC	1 cut(s)	2023
AjnI	CCWGG	3 cut(s)	1368, 1976, 2326
AjuI	GAANNNNNNNTTGG	2 cut(s)	1042, 1074
Alw21I	GWGCWC	4 cut(s)	417, 2038, 2349, 2665
Alw26I	GTCTC	6 cut(s)	310, 367, 1476, 1965, 2231, 2610
AlwI	GGATC	5 cut(s)	1523, 1580, 1707, 1990, 2547
AlwNI	CAGNNNCTG	1 cut(s)	2180
AoxI	GGCC	7 cut(s)	170, 237, 450, 1325, 1366, 1868, 2586
ApeKI	GCWGC	7 cut(s)	317, 380, 1718, 1879, 1955, 2180, 2452
ApoI	RAATTY	2 cut(s)	243, 2155
AseI	ATTAAT	1 cut(s)	1913
AspLEI	GCGC	5 cut(s)	232, 422, 744, 1844, 2028
AspS9I	GGNCC	5 cut(s)	171, 238, 1325, 1366, 1762
AsuHPI	GGTGA	7 cut(s)	10, 193, 394, 629, 745, 1694, 1793
AsuII	TTCGAA	3 cut(s)	482, 642, 890
AvaII	GGWCC	1 cut(s)	1762
BaeGI	GKGCMC	2 cut(s)	1506, 2642
BalI	TGGCCA	1 cut(s)	2588
BanII	GRGCYC	2 cut(s)	417, 2038
BauI	CACGAG	1 cut(s)	2688
BbsI	GAAGAC	2 cut(s)	627, 1676
Bbv12I	GWGCWC	4 cut(s)	417, 2038, 2349, 2665
BbvCI	CCTCAGC	1 cut(s)	906
BbvI	GCAGC	7 cut(s)	329, 367, 1730, 1891, 1967, 2167, 2464
BceAI	ACGGC	5 cut(s)	95, 319, 368, 627, 2454
BciT130I	CCWGG	3 cut(s)	1370, 1978, 2328
BclI	TGATCA	1 cut(s)	2146
BcoDI	GTCTC	6 cut(s)	310, 367, 1476, 1965, 2231, 2610
BcuI	ACTAGT	1 cut(s)	2429
BfmI	CTRYAG	2 cut(s)	567, 2057
BglI	GCCNNNNNGGC	1 cut(s)	449
BglII	AGATCT	1 cut(s)	2014
BmcAI	AGTACT	1 cut(s)	528
Bme1390I	CCNGG	3 cut(s)	1370, 1978, 2328
Bme18I	GGWCC	1 cut(s)	1762
BmgBI	CACGTC	1 cut(s)	2023
BmgT120I	GGNCC	5 cut(s)	171, 238, 1325, 1366, 1762
BmiI	GGNNCC	6 cut(s)	792, 846, 1303, 1582, 1763, 2468
BmrFI	CCNGG	3 cut(s)	1370, 1978, 2328
BmsI	GCATC	7 cut(s)	661, 802, 1162, 1190, 1382, 2198, 2479
BpiI	GAAGAC	2 cut(s)	627, 1676
BplI	GAGNNNNNCTC	2 cut(s)	347, 379
BpmI	CTGGAG	3 cut(s)	578, 1719, 2349
Bpu10I	CCTNAGC	2 cut(s)	906, 1941
Bpu14I	TTCGAA	3 cut(s)	482, 642, 890
BpuEI	CTTGAG	4 cut(s)	551, 1166, 1496, 2687
Bsa29I	ATCGAT	1 cut(s)	1109
BsaBI	GATNNNNATC	1 cut(s)	1986
BsaHI	GRCGYC	1 cut(s)	360
BsaI	GGTCTC	1 cut(s)	310
BsaJI	CCNNGG	5 cut(s)	213, 300, 1430, 1722, 1977
BsaXI	ACNNNNNCTCC	4 cut(s)	176, 206, 953, 983
Bsc4I	CCNNNNNNNGG	6 cut(s)	1334, 1595, 2005, 2219, 2267, 2476
Bse1I	ACTGG	6 cut(s)	96, 1185, 1271, 1339, 1705, 1938
Bse3DI	GCAATG	1 cut(s)	515
Bse8I	GATNNNNATC	1 cut(s)	1986
BseBI	CCWGG	3 cut(s)	1370, 1978, 2328
BseCI	ATCGAT	1 cut(s)	1109
BseDI	CCNNGG	5 cut(s)	213, 300, 1430, 1722, 1977
BseGI	GGATG	9 cut(s)	676, 759, 817, 1038, 1072, 1132, 1181, 2640, 2683
BseJI	GATNNNNATC	1 cut(s)	1986
BseLI	CCNNNNNNNGG	6 cut(s)	1334, 1595, 2005, 2219, 2267, 2476
BseMI	GCAATG	1 cut(s)	515
BseMII	CTCAG	7 cut(s)	888, 897, 1040, 1335, 1955, 2308, 2621
BseNI	ACTGG	6 cut(s)	96, 1185, 1271, 1339, 1705, 1938
BseRI	GAGGAG	1 cut(s)	46
BseSI	GKGCMC	2 cut(s)	1506, 2642
BseXI	GCAGC	7 cut(s)	329, 367, 1730, 1891, 1967, 2167, 2464
Bsh1236I	CGCG	6 cut(s)	230, 302, 1641, 1673, 1842, 2426
BshFI	GGCC	7 cut(s)	172, 239, 452, 1327, 1368, 1870, 2588
BshVI	ATCGAT	1 cut(s)	1109
BsiHKAI	GWGCWC	4 cut(s)	417, 2038, 2349, 2665
BslFI	GGGAC	3 cut(s)	2304, 2353, 2423
BslI	CCNNNNNNNGG	6 cut(s)	1334, 1595, 2005, 2219, 2267, 2476
BsmAI	GTCTC	6 cut(s)	310, 367, 1476, 1965, 2231, 2610
BsmBI	CGTCTC	2 cut(s)	367, 1965
BsmFI	GGGAC	3 cut(s)	2304, 2353, 2423
BsmI	GAATGC	2 cut(s)	898, 2425
BsnI	GGCC	7 cut(s)	172, 239, 452, 1327, 1368, 1870, 2588
Bso31I	GGTCTC	1 cut(s)	310
Bsp119I	TTCGAA	3 cut(s)	482, 642, 890
Bsp1286I	GDGCHC	6 cut(s)	417, 1506, 2038, 2349, 2642, 2665
Bsp1407I	TGTACA	2 cut(s)	2626, 2732
Bsp19I	CCATGG	3 cut(s)	213, 1430, 1722
BspANI	GGCC	7 cut(s)	172, 239, 452, 1327, 1368, 1870, 2588
BspCNI	CTCAG	7 cut(s)	889, 898, 1039, 1334, 1954, 2307, 2620
BspDI	ATCGAT	1 cut(s)	1109
BspFNI	CGCG	6 cut(s)	230, 302, 1641, 1673, 1842, 2426
BspHI	TCATGA	1 cut(s)	1990
BspLI	GGNNCC	6 cut(s)	792, 846, 1303, 1582, 1763, 2468
BspPI	GGATC	5 cut(s)	1523, 1580, 1707, 1990, 2547
BspT104I	TTCGAA	3 cut(s)	482, 642, 890
BspTNI	GGTCTC	1 cut(s)	310
BsrBI	CCGCTC	1 cut(s)	197
BsrDI	GCAATG	1 cut(s)	515
BsrGI	TGTACA	2 cut(s)	2626, 2732
BsrI	ACTGG	6 cut(s)	96, 1185, 1271, 1339, 1705, 1938
BssECI	CCNNGG	5 cut(s)	213, 300, 1430, 1722, 1977
BssNI	GRCGYC	1 cut(s)	360
BssSI	CACGAG	1 cut(s)	2688
BssT1I	CCWWGG	3 cut(s)	213, 1430, 1722
Bst2BI	CACGAG	1 cut(s)	2688
Bst2UI	CCWGG	3 cut(s)	1370, 1978, 2328
Bst4CI	ACNGT	8 cut(s)	725, 1470, 2210, 2314, 2344, 2476, 2536, 2737
Bst6I	CTCTTC	3 cut(s)	83, 644, 1968
BstACI	GRCGYC	1 cut(s)	360
BstAPI	GCANNNNNTGC	1 cut(s)	585
BstAUI	TGTACA	2 cut(s)	2626, 2732
BstBI	TTCGAA	3 cut(s)	482, 642, 890
BstC8I	GCNNGC	4 cut(s)	129, 1407, 1418, 2661
BstDSI	CCRYGG	4 cut(s)	213, 300, 1430, 1722
BstENI	CCTNNNNNAGG	1 cut(s)	1593
BstF5I	GGATG	9 cut(s)	676, 759, 817, 1038, 1072, 1132, 1181, 2640, 2683
BstFNI	CGCG	6 cut(s)	230, 302, 1641, 1673, 1842, 2426
BstHHI	GCGC	5 cut(s)	232, 422, 744, 1844, 2028
BstMAI	GTCTC	6 cut(s)	310, 367, 1476, 1965, 2231, 2610
BstNI	CCWGG	3 cut(s)	1370, 1978, 2328
BstSCI	CCNGG	3 cut(s)	1368, 1976, 2326
BstSFI	CTRYAG	2 cut(s)	567, 2057
BstSLI	GKGCMC	2 cut(s)	1506, 2642
BstUI	CGCG	6 cut(s)	230, 302, 1641, 1673, 1842, 2426
BstV1I	GCAGC	7 cut(s)	329, 367, 1730, 1891, 1967, 2167, 2464
BstV2I	GAAGAC	2 cut(s)	627, 1676
BstX2I	RGATCY	4 cut(s)	1515, 1995, 2014, 2539
BstXI	CCANNNNNNTGG	1 cut(s)	214
BstYI	RGATCY	4 cut(s)	1515, 1995, 2014, 2539
Bsu15I	ATCGAT	1 cut(s)	1109
BsuRI	GGCC	7 cut(s)	172, 239, 452, 1327, 1368, 1870, 2588
BsuTUI	ATCGAT	1 cut(s)	1109
BtgI	CCRYGG	4 cut(s)	213, 300, 1430, 1722
BtgZI	GCGATG	2 cut(s)	298, 2575
BtrI	CACGTC	1 cut(s)	2023
BtsCI	GGATG	9 cut(s)	676, 759, 817, 1038, 1072, 1132, 1181, 2640, 2683
BtsIMutI	CAGTG	5 cut(s)	103, 1034, 1278, 2349, 2541
Cac8I	GCNNGC	4 cut(s)	129, 1407, 1418, 2661
CaiI	CAGNNNCTG	1 cut(s)	2180
CciI	TCATGA	1 cut(s)	1990
CfoI	GCGC	5 cut(s)	232, 422, 744, 1844, 2028
Cfr13I	GGNCC	5 cut(s)	171, 238, 1325, 1366, 1762
Cfr42I	CCGCGG	1 cut(s)	303
ClaI	ATCGAT	1 cut(s)	1109
CseI	GACGC	1 cut(s)	1947
Csp6I	GTAC	9 cut(s)	508, 527, 778, 835, 2351, 2472, 2516, 2627, 2733
CviQI	GTAC	9 cut(s)	508, 527, 778, 835, 2351, 2472, 2516, 2627, 2733
DraI	TTTAAA	1 cut(s)	2676
EaeI	YGGCCR	1 cut(s)	2586
Eam1104I	CTCTTC	3 cut(s)	83, 644, 1968
EarI	CTCTTC	3 cut(s)	83, 644, 1968
EciI	GGCGGA	6 cut(s)	97, 214, 298, 321, 2479, 2573
Ecl136II	GAGCTC	2 cut(s)	415, 2036
Eco130I	CCWWGG	3 cut(s)	213, 1430, 1722
Eco147I	AGGCCT	1 cut(s)	452
Eco24I	GRGCYC	2 cut(s)	417, 2038
Eco31I	GGTCTC	1 cut(s)	310
Eco32I	GATATC	2 cut(s)	1107, 1285
Eco47I	GGWCC	1 cut(s)	1762
Eco53kI	GAGCTC	2 cut(s)	415, 2036
Eco57I	CTGAAG	4 cut(s)	488, 1575, 2093, 2235
EcoICRI	GAGCTC	2 cut(s)	415, 2036
EcoNI	CCTNNNNNAGG	1 cut(s)	1593
EcoO109I	RGGNCCY	2 cut(s)	1325, 1762
EcoRII	CCWGG	3 cut(s)	1368, 1976, 2326
EcoRV	GATATC	2 cut(s)	1107, 1285
EcoT14I	CCWWGG	3 cut(s)	213, 1430, 1722
EcoT22I	ATGCAT	1 cut(s)	1795
EcoT38I	GRGCYC	2 cut(s)	417, 2038
ErhI	CCWWGG	3 cut(s)	213, 1430, 1722
Esp3I	CGTCTC	2 cut(s)	367, 1965
FalI	AAGNNNNNCTT	8 cut(s)	870, 902, 984, 1016, 1536, 1568, 2091, 2123
FaqI	GGGAC	3 cut(s)	2304, 2353, 2423
FauI	CCCGC	2 cut(s)	1331, 2448
FbaI	TGATCA	1 cut(s)	2146
FokI	GGATG	9 cut(s)	683, 766, 824, 1045, 1079, 1119, 1168, 2647, 2670
FriOI	GRGCYC	2 cut(s)	417, 2038
FspI	TGCGCA	1 cut(s)	2027
GlaI	GCGC	5 cut(s)	231, 421, 743, 1843, 2027
GsuI	CTGGAG	3 cut(s)	578, 1719, 2349
HaeIII	GGCC	7 cut(s)	172, 239, 452, 1327, 1368, 1870, 2588
HgaI	GACGC	1 cut(s)	1947
HhaI	GCGC	5 cut(s)	232, 422, 744, 1844, 2028
Hin1I	GRCGYC	1 cut(s)	360
Hin6I	GCGC	5 cut(s)	230, 420, 742, 1842, 2026
HinP1I	GCGC	5 cut(s)	230, 420, 742, 1842, 2026
HincII	GTYRAC	1 cut(s)	1645
HindII	GTYRAC	1 cut(s)	1645
HindIII	AAGCTT	3 cut(s)	278, 1424, 2565
HinfI	GANTC	8 cut(s)	497, 601, 1148, 1547, 1784, 2127, 2292, 2603
HpaI	GTTAAC	1 cut(s)	1645
HphI	GGTGA	7 cut(s)	10, 193, 394, 629, 745, 1694, 1793
Hpy166II	GTNNAC	2 cut(s)	376, 1645
Hpy8I	GTNNAC	2 cut(s)	376, 1645
Hpy99I	CGWCG	1 cut(s)	835
HpyCH4III	ACNGT	8 cut(s)	725, 1470, 2210, 2314, 2344, 2476, 2536, 2737
HpyCH4IV	ACGT	4 cut(s)	360, 833, 2022, 2443
HpySE526I	ACGT	4 cut(s)	360, 833, 2022, 2443
Hsp92I	GRCGYC	1 cut(s)	360
HspAI	GCGC	5 cut(s)	230, 420, 742, 1842, 2026
Ksp22I	TGATCA	1 cut(s)	2146
KspAI	GTTAAC	1 cut(s)	1645
KspI	CCGCGG	1 cut(s)	303
LmnI	GCTCC	4 cut(s)	844, 1091, 1146, 2466
Lsp1109I	GCAGC	7 cut(s)	329, 367, 1730, 1891, 1967, 2167, 2464
LweI	GCATC	7 cut(s)	661, 802, 1162, 1190, 1382, 2198, 2479
MaeII	ACGT	4 cut(s)	360, 833, 2022, 2443
MaeIII	GTNAC	5 cut(s)	25, 112, 733, 1657, 1781
MbiI	CCGCTC	1 cut(s)	197
MfeI	CAATTG	3 cut(s)	1812, 2093, 2184
MflI	RGATCY	4 cut(s)	1515, 1995, 2014, 2539
MhlI	GDGCHC	6 cut(s)	417, 1506, 2038, 2349, 2642, 2665
MlsI	TGGCCA	1 cut(s)	2588
MluNI	TGGCCA	1 cut(s)	2588
MlyI	GAGTC	5 cut(s)	1157, 1778, 2121, 2286, 2612
Mox20I	TGGCCA	1 cut(s)	2588
Mph1103I	ATGCAT	1 cut(s)	1795
MscI	TGGCCA	1 cut(s)	2588
MseI	TTAA	8 cut(s)	705, 1644, 1679, 1821, 1865, 1913, 2367, 2675
MslI	CAYNNNNRTG	2 cut(s)	212, 878
Msp20I	TGGCCA	1 cut(s)	2588
MspA1I	CMGCKG	3 cut(s)	302, 2455, 2513
MspR9I	CCNGG	3 cut(s)	1370, 1978, 2328
MunI	CAATTG	3 cut(s)	1812, 2093, 2184
Mva1269I	GAATGC	2 cut(s)	898, 2425
MvaI	CCWGG	3 cut(s)	1370, 1978, 2328
MvnI	CGCG	6 cut(s)	230, 302, 1641, 1673, 1842, 2426
NcoI	CCATGG	3 cut(s)	213, 1430, 1722
NlaIV	GGNNCC	6 cut(s)	792, 846, 1303, 1582, 1763, 2468
NmeAIII	GCCGAG	1 cut(s)	873
NmuCI	GTSAC	5 cut(s)	25, 112, 733, 1657, 1781
NsbI	TGCGCA	1 cut(s)	2027
NsiI	ATGCAT	1 cut(s)	1795
NspV	TTCGAA	3 cut(s)	482, 642, 890
PagI	TCATGA	1 cut(s)	1990
PceI	AGGCCT	1 cut(s)	452
PcsI	WCGNNNNNNNCGW	1 cut(s)	162
PctI	GAATGC	2 cut(s)	898, 2425
PfeI	GAWTC	3 cut(s)	497, 601, 1547
PleI	GAGTC	5 cut(s)	1156, 1778, 2121, 2286, 2611
PpsI	GAGTC	5 cut(s)	1156, 1778, 2121, 2286, 2611
PpuMI	RGGWCCY	1 cut(s)	1762
PshBI	ATTAAT	1 cut(s)	1913
Psp124BI	GAGCTC	2 cut(s)	417, 2038
Psp5II	RGGWCCY	1 cut(s)	1762
Psp6I	CCWGG	3 cut(s)	1368, 1976, 2326
PspGI	CCWGG	3 cut(s)	1368, 1976, 2326
PspN4I	GGNNCC	6 cut(s)	792, 846, 1303, 1582, 1763, 2468
PspPI	GGNCC	5 cut(s)	171, 238, 1325, 1366, 1762
PspPPI	RGGWCCY	1 cut(s)	1762
PstNI	CAGNNNCTG	1 cut(s)	2180
PsuI	RGATCY	4 cut(s)	1515, 1995, 2014, 2539
RsaI	GTAC	9 cut(s)	509, 528, 779, 836, 2352, 2473, 2517, 2628, 2734
RsaNI	GTAC	9 cut(s)	508, 527, 778, 835, 2351, 2472, 2516, 2627, 2733
RseI	CAYNNNNRTG	2 cut(s)	212, 878
SacI	GAGCTC	2 cut(s)	417, 2038
SacII	CCGCGG	1 cut(s)	303
SaqAI	TTAA	8 cut(s)	705, 1644, 1679, 1821, 1865, 1913, 2367, 2675
Sau96I	GGNCC	5 cut(s)	171, 238, 1325, 1366, 1762
ScaI	AGTACT	1 cut(s)	528
SchI	GAGTC	5 cut(s)	1157, 1778, 2121, 2286, 2612
ScrFI	CCNGG	3 cut(s)	1370, 1978, 2328
SduI	GDGCHC	6 cut(s)	417, 1506, 2038, 2349, 2642, 2665
SfaNI	GCATC	7 cut(s)	661, 802, 1162, 1190, 1382, 2198, 2479
SfcI	CTRYAG	2 cut(s)	567, 2057
Sfr303I	CCGCGG	1 cut(s)	303
SfuI	TTCGAA	3 cut(s)	482, 642, 890
SgrBI	CCGCGG	1 cut(s)	303
SinI	GGWCC	1 cut(s)	1762
SmiMI	CAYNNNNRTG	2 cut(s)	212, 878
SmlI	CTYRAG	4 cut(s)	530, 1145, 1475, 2666
SmoI	CTYRAG	4 cut(s)	530, 1145, 1475, 2666
SpeI	ACTAGT	1 cut(s)	2429
SseBI	AGGCCT	1 cut(s)	452
SspI	AATATT	1 cut(s)	1525
SstI	GAGCTC	2 cut(s)	417, 2038
StuI	AGGCCT	1 cut(s)	452
StyD4I	CCNGG	3 cut(s)	1368, 1976, 2326
StyI	CCWWGG	3 cut(s)	213, 1430, 1722
TaaI	ACNGT	8 cut(s)	725, 1470, 2210, 2314, 2344, 2476, 2536, 2737
TaiI	ACGT	4 cut(s)	363, 836, 2025, 2446
TatI	WGTACW	3 cut(s)	526, 2626, 2732
TauI	GCSGC	6 cut(s)	104, 230, 299, 302, 446, 2426
TfiI	GAWTC	3 cut(s)	497, 601, 1547
Tru1I	TTAA	8 cut(s)	705, 1644, 1679, 1821, 1865, 1913, 2367, 2675
Tru9I	TTAA	8 cut(s)	705, 1644, 1679, 1821, 1865, 1913, 2367, 2675
TscAI	CASTG	5 cut(s)	103, 1034, 1278, 2349, 2541
TseFI	GTSAC	5 cut(s)	25, 112, 733, 1657, 1781
TseI	GCWGC	7 cut(s)	317, 380, 1718, 1879, 1955, 2180, 2452
Tsp45I	GTSAC	5 cut(s)	25, 112, 733, 1657, 1781
TspDTI	ATGAA	9 cut(s)	60, 829, 1049, 1370, 1581, 1955, 2241, 2718, 2735
TspGWI	ACGGA	4 cut(s)	13, 166, 1230, 1528
TspRI	CASTG	5 cut(s)	103, 1034, 1278, 2349, 2541
VpaK11BI	GGWCC	1 cut(s)	1762
VspI	ATTAAT	1 cut(s)	1913
XagI	CCTNNNNNAGG	1 cut(s)	1593
XapI	RAATTY	2 cut(s)	243, 2155
XbaI	TCTAGA	2 cut(s)	283, 1190
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1360, 1428
ZraI	GACGTC	1 cut(s)	361
ZrmI	AGTACT	1 cut(s)	528
Zsp2I	ATGCAT	1 cut(s)	1795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.