Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005368

Physical Location & Seq

Genomic Coordinates Reverse (-)

89513640 .. 89519886

Gene Structure 6247 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00068290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3723 bp

ATGCAAAAACCAGTGCTCACCTCCCTCACTCCATTCAACCTAACCTTCTTCCTCTCCTCCCCAAAATCTTCATACCTCTTCCCCAAACCCACCGCCAGTGCCGCCTCCGCCGTCACTAACTTCTCCCTCGCCCATTCCCTCCAATCCGAAACCCTCGAAATCCTCGAATGGCCCTCCGTCTGCAACCACCTCTCCGCTCTCGCCTCCACCCCCATGGGATTCTCCGCCGCGCAGAACGCCCAAATTCCCTTCGGAAAGTCCAAGTCCGAGAGCCAGAAGCTTCTAGATCAAACCGCCGCCGCGGTCTCCGCCATCGCAGCCATCGGTTCTCCGCCGTCCGATCTCCACTCCATCGAGGACGTCTCGGAGATAGTAAACGCCGCCGTTTTGGGCAAATTGCTCACCATCAACGAGCTCTGCGCTGTTCGTAGAACACTCATAGCCGCAAAGGCCTTGTTCCAGAAGCTGAAGGAACTAGCTTCGAAAGCAGAAGCAGATTCTAATAGGTACTTGCCATTGCTTGAAGTACTTGAGGATTGTGATTTTCTAGTGAAGCTGGAGAGAACTATAGGGTTGTGCATAGACTGCAATCTTTCGGAGATTCTCGACACGGCTAGTGAAGACTTGGAGATCATTAGGTTCGAAAAGAAGAGGAACATGGAGAAATTGGATGCTTTGTTGAAGGAAATGTCGTCTAAGATATTTAAGGCTGGTGGGATTGACAGTCCTTTGGTGACGAAGCGCAGGGCGAGGATGTGCGTGGGTGTTAGAGCTAGGTACAAGTATTTGGTTCCAGATGGCGTGGTGCTGGATGCTAGTAGTTCTGGTGCGACGTACTTCATGGAGCCGAGTGAGGCGGTGGAGTTGAATAACATGGAAGTGAGGCTTTCGAATGCTGAGAAGGCTGAGGAGATAGGCATTTTGAGCTTCCTTACGTCGGAAATTGCAAAATCGGAAGCGGAGATAGTGTATCTGTTAGATAAGATTGTAGAAATTGATCTTGCTTTTGCTAGAGCTGCGTACGCTCAGTGGATGAATGGGGTATGCCCAATTTTCAGTTCTAAGGATGGCAAGGATTTAGATAATGGTGGAGCTGAAATTGGGATATCGGTAGATATTGAAGGAATACAACATCCTTTGCTCCTTGAGCCATCTCTTAGAAGTTTGTCTGATGCCCTTGCATCCAGTTCTAGAAGCGCATTTTCCTCCAAAGACGGAAATGATGTAAAAATGGTCTATCGAAGTTTATCTAGTGGTGTATCTGATTTTCCAGTGCCAATAGATATCAAAATTGGATATGGAACCAGAGTGGTTGTAATCTCAGGGCCTAATACTGGTGGGAAAACAGCTTCCATGAAAACTTTGGGCCTAGCATCTCTTATGTCGAAAGCTGGAATGTATCTGCCTGCTAAAAGCCAGCCAAAGCTTCCATGGTTTGATCTTGTTCTAGTTGATATCGGAGATCAACAGTCTCTTGAGCAAAGCCTCTCAACATTTAGTGGACACATATCACGGATCTGTAATATTTTGGAAGTCGCCTCAAAAGAATCACTTGTCCTCATTGATGAAATTGGTAGTGGAACCGATCCTTCAGAAGGTGTGGCTCTTTCTACCAGCATCTTGCAATATCTCAAAGATCGTGTTAACTTAGCTGTTGTGACTACTCATTACGCGGATTTAAGTCTTCTAAAAGAAAAGGATCACCAGTTTGAAAATGCAGCCATGGAGTTTTCTCCAGAAACCCTACAACCAACTTATCGGGTCCTATGGGGAAGTGTTGGTGACTCAAATGCATTGAGTATTGCTAAATCAATTGGTTTTAATCAACAAATAATTGAACGTGCACAGGATTGGGTGGAAAGATTAAGGCCAGAAAAGCAGCAAGAGCGTAAAGGTTTGCTATATCGGTCATTAATAGAGGAAAGAAACAGACTGGAAGCTCAGGCTAAGATGGCAGCGTCTCTTCATTCAGAAACCAGGGATATTTATCATGAGATCCAAGATGAGGCAGAAGATCTTGACGTGCGCAAGAGAGCTCTTATGGCAAAGGAAACCCTACAGGTTCGGGAAGAACTGAAGATTGTCAAATCTCAATTAGAAGCTGTGCTTCAGGAGTTTGATAACCGACTCAAAACTGCTGGTGCCGATCAGTTGAATTTGCTGATTAAGAAATCAGAAGCTGCAGTTGCATCTATTATTGAGGCTCACAGTCCTGAAGATGGTTTCTTGGTTAATGAGACATCTGAAACTTCATACACACCTCGATTTGGGGAACAAGTCTATTTGAAGGGACTCAGAGATAAGATAGCTACTGTAGTTGAAGCACCTGGAGATGATGGGACAGTGCTCGTACAATATGGTAAAATTAAGGTTCGGTTGAAGAATAATGAGATCAGAGCTATTCCTAGTAGTGATAAAAATGCCGTGACTAGTTCTGTCCCACGTTTGAAGCAGCGGGTTTGGCGGAGCCGTACTGCAGAAAACAAAGATGGGGAGGTTTCTTATAGTCCAGCAGTACAAACATCAAAGAACACAGTGGATCTTCGTGGCATGAGGGCGGAGGAAGCTTCTTACAATCTGGATATGGCTATCGCTGCTAGAGAGTCTCAGTCAGTTCTTTTTGTTGTACATGGCATGGGCACAGGGGTTGTCAAAGAGCGAGCACTTGAGATTTTAAAGAAACATCCTCGAGTAGCCAAGTATGAAGCGGAAAGTCCAATGAATTATGGTTTTCTGAAACCTGATAAGATGCAGACATTACCAGAAGCAAATCACAAGATCACAAGGCTGCACAAGAAGACAACACACACTCCAGCACAAGACAATTGGGAGACAACTCGTGCTCTCTTAGCCAAGCTTATAGCTCAGAGCAGACTACACGTCTCGTCCCATGTAAACCTTGGAGCTGCCACATGCATAGTCCTTACGCAACACTCTTTGTTTTGCTGGAACATGTTGACGGTTTTCGACAAGACAACAGGAAACCCTCCTGTAGAGCTGGGGCTTGCTTCGATTGGGCCAGAGAAGGCAAAGACCAGAGAGGAAATCTTGAAGCTTTTCCAATCACCACGTCGTGAGTGCACCGTATGCTACCTTTCAGATGGGAATTTCATGGCTTTGTCTCATGATAATGAACATCAGTCTCACCCAAGGTCAATTGTAGTCATAGACGATGTCTTCTGCATTTTCATTGGGACTTTGGAGAACACATCTGACCTGAGACGATACTATGGCCTTCCAAGACAAGCAACAGAAGCCATGGTTGTGGTTGAAGTCTATAAAGTCCTAAGAGATCGAGCACCCTACCCACCTGATCAAGTCATCAAAGATCTGCAGGGAAAATTTGCATTTATCCTCTTCGATGCTAGATCTGGTACCCTTTTTGCAGCCAGGGACCGAGATGGAAGCGTTGAACTTCACTGGGGAATGGCAGGTGATGGGTCTCTAGTCTGCTCCCACAAACTCAACATCATTAGAGAAGCTTGTGGGAAATGTTGTGCGCCTTTCCCTCCAGGCTGCATATTCACAAATGGAAGTGGTTTGATGAGCTTTGTTCATCCACTGCACAAGGTAAGGGCCATTGCACCCGAAGACGACAGTGGCAACATTTGTGGAGTTGCTTTTCAGGTGGATCTGTTCACACGAATCCCCAGCATTCCACGCACCGGTAGCGGACAGAATTGGGCGGATGCAACTGCTGTCAAGGAAGGAGATCACTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko03430 map03430

Pfam Domains

Protein Families

MutS_V Smr

Protein Analysis

1240

Amino Acids

136.42

Weight (kDa)

6.04

Isoelectric Point (pI)

42.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_V	PF00488	438 - 622	5.4e-34	MutS domain V
MSSS	PF20297	757 - 798	2.5e-07	MutS2 and Smr-associated SH3 domain
Smr	PF01713	846 - 907	9.1e-10	Smr domain
DUF3700	PF12481	986 - 1210	9.8e-83	Aluminium induced protein
GATase_7	PF13537	1057 - 1152	3.4e-08	Glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005368)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65070 AT1G65070 AT1G65070 AT1G65070
fragaria_vesca	FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390 FvH4_3g09390
malus_domestica	MD00G1095300.v1.1
prunus_persica	Prupe.4G045200_v2.0.a1
pyrus_communis	pycom10g24790
rosa_chinensis	RchiOBHm_Chr5g0008191
rosa_laevigata	RLG00000031545
rosa_multiflora	Rmu_co8487121.1_g000001 Rmu_sc0001352.1_g000007 Rmu_sc0001352.1_g000008 Rmu_sc0001352.1_g000029 Rmu_sc0001626.1_g000010
rosa_roxburghii	Rroxscaffold_1G00068290
rosa_rugosa	Rorug04G0434000 Rorug04G0434100
rosa_samantha	Rh5AG062400 Rh5BG058700 Rh5CG069200 Rh5DG058100
rosa_wichuraiana	Rw5G005850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3422
AatII	GACGTC	1 cut(s)	363
Acc16I	TGCGCA	1 cut(s)	2027
Acc36I	ACCTGC	1 cut(s)	3422
Acc65I	GGTACC	1 cut(s)	3374
AccB1I	GGYRCC	2 cut(s)	2141, 3374
AccBSI	CCGCTC	1 cut(s)	197
AccII	CGCG	3 cut(s)	230, 302, 1673
AclWI	GGATC	6 cut(s)	1523, 1580, 1707, 1990, 2547, 3639
AcsI	RAATTY	4 cut(s)	243, 2155, 3106, 3341
AcuI	CTGAAG	5 cut(s)	488, 1575, 2093, 2096, 2235
AcyI	GRCGYC	1 cut(s)	360
AdeI	CACNNNGTG	1 cut(s)	3074
AfiI	CCNNNNNNNGG	7 cut(s)	937, 1334, 1595, 2005, 2219, 2267, 3665
AflIII	ACRYGT	2 cut(s)	2878, 2952
AgeI	ACCGGT	1 cut(s)	3665
AhdI	GACNNNNNGTC	1 cut(s)	2879
AhlI	ACTAGT	1 cut(s)	2429
AjiI	CACGTC	3 cut(s)	2023, 2881, 3071
AjnI	CCWGG	4 cut(s)	1976, 2326, 3389, 3511
AjuI	GAANNNNNNNTTGG	2 cut(s)	1042, 1074
Alw21I	GWGCWC	9 cut(s)	18, 417, 1846, 2038, 2349, 2665, 2845, 3083, 3301
Alw44I	GTGCAC	2 cut(s)	1842, 3079
AlwI	GGATC	6 cut(s)	1523, 1580, 1707, 1990, 2547, 3639
AlwNI	CAGNNNCTG	2 cut(s)	466, 2180
Ama87I	CYCGRG	1 cut(s)	2688
AoxI	GGCC	8 cut(s)	170, 450, 1325, 1366, 1868, 3017, 3232, 3576
ApaLI	GTGCAC	2 cut(s)	1842, 3079
ApoI	RAATTY	4 cut(s)	243, 2155, 3106, 3341
AseI	ATTAAT	1 cut(s)	1913
AsiGI	ACCGGT	1 cut(s)	3665
Asp700I	GAANNNNTTC	1 cut(s)	3056
Asp718I	GGTACC	1 cut(s)	3374
AspLEI	GCGC	6 cut(s)	232, 422, 744, 1199, 2028, 3502
AspS9I	GGNCC	7 cut(s)	171, 1325, 1366, 1762, 3017, 3394, 3576
AsuHPI	GGTGA	8 cut(s)	10, 394, 745, 1694, 1793, 3057, 3137, 3446
AsuII	TTCGAA	3 cut(s)	482, 642, 890
AvaI	CYCGRG	1 cut(s)	2688
AvaII	GGWCC	2 cut(s)	1762, 3394
BaeGI	GKGCMC	3 cut(s)	1846, 2642, 3083
BanI	GGYRCC	2 cut(s)	2141, 3374
BanII	GRGCYC	2 cut(s)	417, 2038
BauI	CACGAG	1 cut(s)	2838
BbsI	GAAGAC	5 cut(s)	627, 1676, 2804, 3169, 3597
Bbv12I	GWGCWC	9 cut(s)	18, 417, 1846, 2038, 2349, 2665, 2845, 3083, 3301
BbvCI	CCTCAGC	1 cut(s)	906
BceAI	ACGGC	6 cut(s)	95, 319, 368, 627, 2408, 2454
BciT130I	CCWGG	4 cut(s)	1978, 2328, 3391, 3513
BclI	TGATCA	1 cut(s)	3313
BcuI	ACTAGT	1 cut(s)	2429
BfmI	CTRYAG	7 cut(s)	567, 2057, 2181, 2313, 2475, 2991, 3332
BfuAI	ACCTGC	1 cut(s)	3422
BglI	GCCNNNNNGGC	1 cut(s)	449
BglII	AGATCT	3 cut(s)	2014, 3328, 3368
BmcAI	AGTACT	1 cut(s)	528
Bme1390I	CCNGG	4 cut(s)	1978, 2328, 3391, 3513
Bme18I	GGWCC	2 cut(s)	1762, 3394
BmeRI	GACNNNNNGTC	1 cut(s)	2879
BmeT110I	CYCGRG	1 cut(s)	2688
BmgBI	CACGTC	3 cut(s)	2023, 2881, 3071
BmgT120I	GGNCC	7 cut(s)	171, 1325, 1366, 1762, 3017, 3394, 3576
BmiI	GGNNCC	9 cut(s)	792, 846, 1303, 1582, 1763, 2143, 2468, 3376, 3395
BmrFI	CCNGG	4 cut(s)	1978, 2328, 3391, 3513
BmrI	ACTGGG	1 cut(s)	3430
BmuI	ACTGGG	1 cut(s)	3430
BpiI	GAAGAC	5 cut(s)	627, 1676, 2804, 3169, 3597
BplI	GAGNNNNNCTC	2 cut(s)	347, 379
BpmI	CTGGAG	5 cut(s)	578, 1719, 2349, 2796, 3495
Bpu10I	CCTNAGC	2 cut(s)	906, 1941
Bpu14I	TTCGAA	3 cut(s)	482, 642, 890
BpuEI	CTTGAG	4 cut(s)	551, 1166, 1496, 2687
BsaBI	GATNNNNATC	1 cut(s)	1986
BsaHI	GRCGYC	1 cut(s)	360
BsaI	GGTCTC	2 cut(s)	310, 3447
BsaJI	CCNNGG	9 cut(s)	213, 300, 1430, 1722, 1977, 2899, 3149, 3258, 3390
BsaWI	WCCGGW	1 cut(s)	3665
BsaXI	ACNNNNNCTCC	6 cut(s)	176, 206, 953, 983, 2794, 2824
Bsc4I	CCNNNNNNNGG	7 cut(s)	937, 1334, 1595, 2005, 2219, 2267, 3665
Bse118I	RCCGGY	1 cut(s)	3665
Bse1I	ACTGG	8 cut(s)	11, 96, 1185, 1271, 1339, 1705, 1938, 3425
Bse3DI	GCAATG	2 cut(s)	515, 3579
Bse8I	GATNNNNATC	1 cut(s)	1986
BseBI	CCWGG	4 cut(s)	1978, 2328, 3391, 3513
BseDI	CCNNGG	9 cut(s)	213, 300, 1430, 1722, 1977, 2899, 3149, 3258, 3390
BseJI	GATNNNNATC	1 cut(s)	1986
BseLI	CCNNNNNNNGG	7 cut(s)	937, 1334, 1595, 2005, 2219, 2267, 3665
BseMI	GCAATG	2 cut(s)	515, 3579
BseMII	CTCAG	9 cut(s)	888, 897, 1040, 1335, 1955, 2308, 2621, 2879, 3209
BseNI	ACTGG	8 cut(s)	11, 96, 1185, 1271, 1339, 1705, 1938, 3425
BseRI	GAGGAG	2 cut(s)	46, 923
BseSI	GKGCMC	3 cut(s)	1846, 2642, 3083
BseYI	CCCAGC	2 cut(s)	2998, 3650
BsgI	GTGCAG	2 cut(s)	2774, 3548
Bsh1236I	CGCG	3 cut(s)	230, 302, 1673
BshFI	GGCC	8 cut(s)	172, 452, 1327, 1368, 1870, 3019, 3234, 3578
BshNI	GGYRCC	2 cut(s)	2141, 3374
BshTI	ACCGGT	1 cut(s)	3665
BsiHKAI	GWGCWC	9 cut(s)	18, 417, 1846, 2038, 2349, 2665, 2845, 3083, 3301
BsiHKCI	CYCGRG	1 cut(s)	2688
BsiSI	CCGG	1 cut(s)	3666
BsiWI	CGTACG	1 cut(s)	1020
BslFI	GGGAC	6 cut(s)	2304, 2353, 2423, 2872, 3208, 3407
BslI	CCNNNNNNNGG	7 cut(s)	937, 1334, 1595, 2005, 2219, 2267, 3665
BsmBI	CGTCTC	4 cut(s)	367, 1965, 2887, 3214
BsmFI	GGGAC	6 cut(s)	2304, 2353, 2423, 2872, 3208, 3407
BsmI	GAATGC	2 cut(s)	898, 3654
BsnI	GGCC	8 cut(s)	172, 452, 1327, 1368, 1870, 3019, 3234, 3578
Bso31I	GGTCTC	2 cut(s)	310, 3447
BsoBI	CYCGRG	1 cut(s)	2688
Bsp119I	TTCGAA	3 cut(s)	482, 642, 890
Bsp1407I	TGTACA	1 cut(s)	2626
Bsp19I	CCATGG	4 cut(s)	213, 1430, 1722, 3258
BspANI	GGCC	8 cut(s)	172, 452, 1327, 1368, 1870, 3019, 3234, 3578
BspCNI	CTCAG	9 cut(s)	889, 898, 1039, 1334, 1954, 2307, 2620, 2878, 3210
BspFNI	CGCG	3 cut(s)	230, 302, 1673
BspHI	TCATGA	2 cut(s)	1990, 3124
BspLI	GGNNCC	9 cut(s)	792, 846, 1303, 1582, 1763, 2143, 2468, 3376, 3395
BspMAI	CTGCAG	3 cut(s)	2185, 2479, 3336
BspMI	ACCTGC	1 cut(s)	3422
BspPI	GGATC	6 cut(s)	1523, 1580, 1707, 1990, 2547, 3639
BspT104I	TTCGAA	3 cut(s)	482, 642, 890
BspT107I	GGYRCC	2 cut(s)	2141, 3374
BspTNI	GGTCTC	2 cut(s)	310, 3447
BsrBI	CCGCTC	1 cut(s)	197
BsrDI	GCAATG	2 cut(s)	515, 3579
BsrFI	RCCGGY	1 cut(s)	3665
BsrGI	TGTACA	1 cut(s)	2626
BsrI	ACTGG	8 cut(s)	11, 96, 1185, 1271, 1339, 1705, 1938, 3425
BssAI	RCCGGY	1 cut(s)	3665
BssECI	CCNNGG	9 cut(s)	213, 300, 1430, 1722, 1977, 2899, 3149, 3258, 3390
BssNI	GRCGYC	1 cut(s)	360
BssSI	CACGAG	1 cut(s)	2838
BssT1I	CCWWGG	6 cut(s)	213, 1430, 1722, 2899, 3149, 3258
Bst2BI	CACGAG	1 cut(s)	2838
Bst2UI	CCWGG	4 cut(s)	1978, 2328, 3391, 3513
Bst4CI	ACNGT	9 cut(s)	725, 1470, 2210, 2314, 2344, 2536, 2962, 3085, 3599
Bst6I	CTCTTC	4 cut(s)	83, 644, 1968, 3362
BstACI	GRCGYC	1 cut(s)	360
BstAPI	GCANNNNNTGC	2 cut(s)	585, 3087
BstAUI	TGTACA	1 cut(s)	2626
BstBI	TTCGAA	3 cut(s)	482, 642, 890
BstC8I	GCNNGC	4 cut(s)	1407, 1418, 2661, 3006
BstDSI	CCRYGG	5 cut(s)	213, 300, 1430, 1722, 3258
BstENI	CCTNNNNNAGG	1 cut(s)	1593
BstFNI	CGCG	3 cut(s)	230, 302, 1673
BstHHI	GCGC	6 cut(s)	232, 422, 744, 1199, 2028, 3502
BstNI	CCWGG	4 cut(s)	1978, 2328, 3391, 3513
BstNSI	RCATGY	2 cut(s)	2916, 2956
BstSCI	CCNGG	4 cut(s)	1976, 2326, 3389, 3511
BstSFI	CTRYAG	7 cut(s)	567, 2057, 2181, 2313, 2475, 2991, 3332
BstSLI	GKGCMC	3 cut(s)	1846, 2642, 3083
BstUI	CGCG	3 cut(s)	230, 302, 1673
BstV2I	GAAGAC	5 cut(s)	627, 1676, 2804, 3169, 3597
BstX2I	RGATCY	7 cut(s)	1515, 1995, 2014, 2539, 3328, 3368, 3631
BstXI	CCANNNNNNTGG	2 cut(s)	214, 3265
BstYI	RGATCY	7 cut(s)	1515, 1995, 2014, 2539, 3328, 3368, 3631
BsuRI	GGCC	8 cut(s)	172, 452, 1327, 1368, 1870, 3019, 3234, 3578
BtgI	CCRYGG	5 cut(s)	213, 300, 1430, 1722, 3258
BtgZI	GCGATG	1 cut(s)	298
BtrI	CACGTC	3 cut(s)	2023, 2881, 3071
BtsI	GCAGTG	1 cut(s)	3560
BtsIMutI	CAGTG	9 cut(s)	18, 103, 1034, 1278, 2349, 2541, 3418, 3560, 3604
BveI	ACCTGC	1 cut(s)	3422
Cac8I	GCNNGC	4 cut(s)	1407, 1418, 2661, 3006
CaiI	CAGNNNCTG	2 cut(s)	466, 2180
CciI	TCATGA	2 cut(s)	1990, 3124
CfoI	GCGC	6 cut(s)	232, 422, 744, 1199, 2028, 3502
Cfr10I	RCCGGY	1 cut(s)	3665
Cfr13I	GGNCC	7 cut(s)	171, 1325, 1366, 1762, 3017, 3394, 3576
Cfr42I	CCGCGG	1 cut(s)	303
CseI	GACGC	1 cut(s)	1947
CspAI	ACCGGT	1 cut(s)	3665
CspCI	CAANNNNNGTGG	2 cut(s)	3592, 3627
DraI	TTTAAA	1 cut(s)	2676
DraIII	CACNNNGTG	1 cut(s)	3074
DriI	GACNNNNNGTC	1 cut(s)	2879
Eam1104I	CTCTTC	4 cut(s)	83, 644, 1968, 3362
Eam1105I	GACNNNNNGTC	1 cut(s)	2879
EarI	CTCTTC	4 cut(s)	83, 644, 1968, 3362
EciI	GGCGGA	7 cut(s)	97, 214, 298, 321, 2479, 2573, 3701
Ecl136II	GAGCTC	2 cut(s)	415, 2036
Eco130I	CCWWGG	6 cut(s)	213, 1430, 1722, 2899, 3149, 3258
Eco147I	AGGCCT	1 cut(s)	452
Eco24I	GRGCYC	2 cut(s)	417, 2038
Eco31I	GGTCTC	2 cut(s)	310, 3447
Eco32I	GATATC	3 cut(s)	1107, 1285, 1456
Eco47I	GGWCC	2 cut(s)	1762, 3394
Eco53kI	GAGCTC	2 cut(s)	415, 2036
Eco57I	CTGAAG	5 cut(s)	488, 1575, 2093, 2096, 2235
Eco88I	CYCGRG	1 cut(s)	2688
EcoICRI	GAGCTC	2 cut(s)	415, 2036
EcoNI	CCTNNNNNAGG	1 cut(s)	1593
EcoO109I	RGGNCCY	2 cut(s)	1325, 1762
EcoRII	CCWGG	4 cut(s)	1976, 2326, 3389, 3511
EcoRV	GATATC	3 cut(s)	1107, 1285, 1456
EcoT14I	CCWWGG	6 cut(s)	213, 1430, 1722, 2899, 3149, 3258
EcoT22I	ATGCAT	2 cut(s)	1795, 2918
EcoT38I	GRGCYC	2 cut(s)	417, 2038
ErhI	CCWWGG	6 cut(s)	213, 1430, 1722, 2899, 3149, 3258
Esp3I	CGTCTC	4 cut(s)	367, 1965, 2887, 3214
FalI	AAGNNNNNCTT	6 cut(s)	870, 902, 1536, 1568, 2091, 2123
FaqI	GGGAC	6 cut(s)	2304, 2353, 2423, 2872, 3208, 3407
FauI	CCCGC	1 cut(s)	2448
FbaI	TGATCA	1 cut(s)	3313
FriOI	GRGCYC	2 cut(s)	417, 2038
FspI	TGCGCA	1 cut(s)	2027
GlaI	GCGC	6 cut(s)	231, 421, 743, 1198, 2027, 3501
GsaI	CCCAGC	2 cut(s)	3002, 3654
GsuI	CTGGAG	5 cut(s)	578, 1719, 2349, 2796, 3495
HaeIII	GGCC	8 cut(s)	172, 452, 1327, 1368, 1870, 3019, 3234, 3578
HapII	CCGG	1 cut(s)	3666
HgaI	GACGC	1 cut(s)	1947
HhaI	GCGC	6 cut(s)	232, 422, 744, 1199, 2028, 3502
Hin1I	GRCGYC	1 cut(s)	360
Hin6I	GCGC	6 cut(s)	230, 420, 742, 1197, 2026, 3500
HinP1I	GCGC	6 cut(s)	230, 420, 742, 1197, 2026, 3500
HincII	GTYRAC	2 cut(s)	1645, 2958
HindII	GTYRAC	2 cut(s)	1645, 2958
HindIII	AAGCTT	6 cut(s)	278, 1424, 2565, 2855, 3053, 3480
HinfI	GANTC	9 cut(s)	219, 497, 601, 1547, 1784, 2127, 2292, 2603, 3645
HpaI	GTTAAC	1 cut(s)	1645
HpaII	CCGG	1 cut(s)	3666
HphI	GGTGA	8 cut(s)	10, 394, 745, 1694, 1793, 3057, 3137, 3446
Hpy166II	GTNNAC	8 cut(s)	376, 1502, 1645, 1844, 2896, 2958, 3081, 3639
Hpy8I	GTNNAC	8 cut(s)	376, 1502, 1645, 1844, 2896, 2958, 3081, 3639
Hpy99I	CGWCG	3 cut(s)	835, 940, 3075
HpyCH4III	ACNGT	9 cut(s)	725, 1470, 2210, 2314, 2344, 2536, 2962, 3085, 3599
HpyCH4IV	ACGT	8 cut(s)	360, 833, 935, 1840, 2022, 2443, 2880, 3070
HpySE526I	ACGT	8 cut(s)	360, 833, 935, 1840, 2022, 2443, 2880, 3070
Hsp92I	GRCGYC	1 cut(s)	360
HspAI	GCGC	6 cut(s)	230, 420, 742, 1197, 2026, 3500
KpnI	GGTACC	1 cut(s)	3378
Ksp22I	TGATCA	1 cut(s)	3313
KspAI	GTTAAC	1 cut(s)	1645
KspI	CCGCGG	1 cut(s)	303
LmnI	GCTCC	6 cut(s)	844, 1091, 1146, 2466, 2903, 3458
MaeII	ACGT	8 cut(s)	360, 833, 935, 1840, 2022, 2443, 2880, 3070
MaeIII	GTNAC	5 cut(s)	112, 733, 1657, 1781, 2425
MbiI	CCGCTC	1 cut(s)	197
MfeI	CAATTG	3 cut(s)	1812, 2824, 3156
MflI	RGATCY	7 cut(s)	1515, 1995, 2014, 2539, 3328, 3368, 3631
MlyI	GAGTC	4 cut(s)	1778, 2121, 2286, 2612
MmeI	TCCRAC	1 cut(s)	918
Mph1103I	ATGCAT	2 cut(s)	1795, 2918
MroXI	GAANNNNTTC	1 cut(s)	3056
MslI	CAYNNNNRTG	4 cut(s)	212, 878, 3129, 3263
MspA1I	CMGCKG	2 cut(s)	302, 2455
MspI	CCGG	1 cut(s)	3666
MspR9I	CCNGG	4 cut(s)	1978, 2328, 3391, 3513
MunI	CAATTG	3 cut(s)	1812, 2824, 3156
Mva1269I	GAATGC	2 cut(s)	898, 3654
MvaI	CCWGG	4 cut(s)	1978, 2328, 3391, 3513
MvnI	CGCG	3 cut(s)	230, 302, 1673
NcoI	CCATGG	4 cut(s)	213, 1430, 1722, 3258
NlaIV	GGNNCC	9 cut(s)	792, 846, 1303, 1582, 1763, 2143, 2468, 3376, 3395
NmeAIII	GCCGAG	1 cut(s)	873
NmuCI	GTSAC	5 cut(s)	112, 733, 1657, 1781, 2425
NsbI	TGCGCA	1 cut(s)	2027
NsiI	ATGCAT	2 cut(s)	1795, 2918
NspI	RCATGY	2 cut(s)	2916, 2956
NspV	TTCGAA	3 cut(s)	482, 642, 890
PaeR7I	CTCGAG	1 cut(s)	2688
PagI	TCATGA	2 cut(s)	1990, 3124
PaqCI	CACCTGC	1 cut(s)	3422
PceI	AGGCCT	1 cut(s)	452
PciI	ACATGT	1 cut(s)	2952
PcsI	WCGNNNNNNNCGW	1 cut(s)	162
PctI	GAATGC	2 cut(s)	898, 3654
PdmI	GAANNNNTTC	1 cut(s)	3056
PfeI	GAWTC	5 cut(s)	219, 497, 601, 1547, 3645
Pfl23II	CGTACG	1 cut(s)	1020
PflFI	GACNNNGTC	1 cut(s)	3173
PinAI	ACCGGT	1 cut(s)	3665
PleI	GAGTC	4 cut(s)	1778, 2121, 2286, 2611
PpsI	GAGTC	4 cut(s)	1778, 2121, 2286, 2611
PpuMI	RGGWCCY	1 cut(s)	1762
PscI	ACATGT	1 cut(s)	2952
PshBI	ATTAAT	1 cut(s)	1913
Psp124BI	GAGCTC	2 cut(s)	417, 2038
Psp5II	RGGWCCY	1 cut(s)	1762
Psp6I	CCWGG	4 cut(s)	1976, 2326, 3389, 3511
PspFI	CCCAGC	2 cut(s)	2998, 3650
PspGI	CCWGG	4 cut(s)	1976, 2326, 3389, 3511
PspLI	CGTACG	1 cut(s)	1020
PspN4I	GGNNCC	9 cut(s)	792, 846, 1303, 1582, 1763, 2143, 2468, 3376, 3395
PspPI	GGNCC	7 cut(s)	171, 1325, 1366, 1762, 3017, 3394, 3576
PspPPI	RGGWCCY	1 cut(s)	1762
PspXI	VCTCGAGB	1 cut(s)	2688
PstI	CTGCAG	3 cut(s)	2185, 2479, 3336
PstNI	CAGNNNCTG	2 cut(s)	466, 2180
PsuI	RGATCY	7 cut(s)	1515, 1995, 2014, 2539, 3328, 3368, 3631
PsyI	GACNNNGTC	1 cut(s)	3173
RseI	CAYNNNNRTG	4 cut(s)	212, 878, 3129, 3263
SacI	GAGCTC	2 cut(s)	417, 2038
SacII	CCGCGG	1 cut(s)	303
Sau96I	GGNCC	7 cut(s)	171, 1325, 1366, 1762, 3017, 3394, 3576
ScaI	AGTACT	1 cut(s)	528
SchI	GAGTC	4 cut(s)	1778, 2121, 2286, 2612
ScrFI	CCNGG	4 cut(s)	1978, 2328, 3391, 3513
SfcI	CTRYAG	7 cut(s)	567, 2057, 2181, 2313, 2475, 2991, 3332
Sfr274I	CTCGAG	1 cut(s)	2688
Sfr303I	CCGCGG	1 cut(s)	303
SfuI	TTCGAA	3 cut(s)	482, 642, 890
SgrBI	CCGCGG	1 cut(s)	303
SinI	GGWCC	2 cut(s)	1762, 3394
SlaI	CTCGAG	1 cut(s)	2688
SmiMI	CAYNNNNRTG	4 cut(s)	212, 878, 3129, 3263
SmlI	CTYRAG	5 cut(s)	530, 1145, 1475, 2666, 2688
SmoI	CTYRAG	5 cut(s)	530, 1145, 1475, 2666, 2688
SpeI	ACTAGT	1 cut(s)	2429
SseBI	AGGCCT	1 cut(s)	452
SspI	AATATT	1 cut(s)	1525
SstI	GAGCTC	2 cut(s)	417, 2038
StuI	AGGCCT	1 cut(s)	452
StyD4I	CCNGG	4 cut(s)	1976, 2326, 3389, 3511
StyI	CCWWGG	6 cut(s)	213, 1430, 1722, 2899, 3149, 3258
TaaI	ACNGT	9 cut(s)	725, 1470, 2210, 2314, 2344, 2536, 2962, 3085, 3599
TaiI	ACGT	8 cut(s)	363, 836, 938, 1843, 2025, 2446, 2883, 3073
TaqII	GACCGA	2 cut(s)	1896, 3411
TatI	WGTACW	3 cut(s)	526, 2515, 2626
TauI	GCSGC	6 cut(s)	104, 230, 299, 302, 383, 446
TfiI	GAWTC	5 cut(s)	219, 497, 601, 1547, 3645
TscAI	CASTG	9 cut(s)	18, 103, 1034, 1278, 2349, 2541, 3425, 3567, 3604
TseFI	GTSAC	5 cut(s)	112, 733, 1657, 1781, 2425
Tsp45I	GTSAC	5 cut(s)	112, 733, 1657, 1781, 2425
TspGWI	ACGGA	3 cut(s)	166, 1230, 1528
TspRI	CASTG	9 cut(s)	18, 103, 1034, 1278, 2349, 2541, 3425, 3567, 3604
Tth111I	GACNNNGTC	1 cut(s)	3173
VneI	GTGCAC	2 cut(s)	1842, 3079
VpaK11BI	GGWCC	2 cut(s)	1762, 3394
VspI	ATTAAT	1 cut(s)	1913
XagI	CCTNNNNNAGG	1 cut(s)	1593
XapI	RAATTY	4 cut(s)	243, 2155, 3106, 3341
XbaI	TCTAGA	2 cut(s)	283, 1190
XceI	RCATGY	2 cut(s)	2916, 2956
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1360, 1428, 2897
XhoI	CTCGAG	1 cut(s)	2688
XmnI	GAANNNNTTC	1 cut(s)	3056
ZraI	GACGTC	1 cut(s)	361
ZrmI	AGTACT	1 cut(s)	528
Zsp2I	ATGCAT	2 cut(s)	1795, 2918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.