MD00G1096100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015527
Physical Location & Seq
Reverse (-)
19986416 .. 19988634
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1096100.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGCATACAAGAAGTAGGAAAAACGTGAGAGTGGAAGAATTGTCGTTGCCTTTGGGAGAATGTATGTCCACTTTTAACCTAGAGAAAGCAGTATGCAACCACGGTTTCTTCATGATGGCTCCAAACCGATGGGTTCCGTCCTCAAAAACACTCGAACGCCCGCTAAGACTTGCAGACTCCACCACATGCGTCACCGTTTCAATCCAACATCCTCCTAATCACACCTCTCTCCTTATTCAAGTCTATGATCACATTCATAACCTCTCATTTGCAGATAAGGATGCTATACTGCAACAAGTGCGTCGAATGCTAAGGATATCGAAGAAAGATGAAAGGGCCATAAGGGAGTATCACGAAGTGCATCCGGAAGCGAAGGAGAAGGGCTGTGGTCGAGTTTTCCGGTCCCCTACTCTCTTTGAAGACCTTGTCAAGTGTATCCTCCTCTGTAACACCAAGTGGTCAAATACATTACAAATGGCTCGATCCCTTTGTGAGCTTCACACTCAAGTATGCAATAACAATAATGCTCCGGCAAAGCAAAAGCAGCCAATCAACCTAAAGAGAAAAAAAGAAATGTTATCTAACACATGGTCGCCATGCAAACTTAACCATCAATTCAGTGACGATGGATCTTCATTTGGGAACTTTCCAAACGCACAAGAACTTGCAGGACTTGATCTGGATACTTTACAAAACTGCAGTACTAGCGTTCTTGGCTACAGAGCGAACACCATTCTGAAACTTGCCAAGATCATGCGACTTGATGATGAACTAGGGTTTCAAGAATCCCTCAACATCGGCTTCAACGACATGTTTCAAAAGTTGAAGGAAATCAAAGGGTTTGGTTCGTTTTCATGCGCCAATGCACTCATGTGCATGGGATATTATCATAAAGTTCATGGGAGGAAGGATTGTGACAAAACAAGCGCCGGAAAAGATGCGGAAGAAATTTATGATAAGTATGCACCGTTCCAGTGCCTGGCATATTTGTCGGAGCTCTTGGATTCATACGAAGGCAAATTTGGGAAGCTGAGTGAATTGCCTGCCTCTAGCTATCATATAATCAGTGGCAGGCTTGAGAGAAAGGGGGAAGAGAAGACCATGATGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HhH-GPD

Protein Analysis

371

Amino Acids

42.17

Weight (kDa)

8.65

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015527)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27954
malus_domestica MD00G1096100.v1.1
prunus_persica Prupe.4G287800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496761
rosa_laevigata RLG00000022458 RLG00000022506
rosa_multiflora Rmu_sc0000542.1_g000009 Rmu_sc0001832.1_g000011
rosa_roxburghii Rroxscaffold_6G00388030
rosa_rugosa Rorug03G0286800
rosa_samantha Rh3AG331200 Rh3BG367800 Rh3CG363900 Rh3DG367200
rosa_wichuraiana Rw0G004010 Rw3G029000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 979
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 2 cut(s) 161, 941
AclWI GGATC 2 cut(s) 477, 637
AcsI RAATTY 2 cut(s) 948, 1019
AdeI CACNNNGTG 2 cut(s) 358, 456
AfaI GTAC 1 cut(s) 703
AfiI CCNNNNNNNGG 1 cut(s) 979
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 7 cut(s) 201, 239, 419, 782, 805, 818, 826
AjnI CCWGG 1 cut(s) 978
AjuI GAANNNNNNNTTGG 2 cut(s) 1005, 1037
AleI CACNNNNGTG 1 cut(s) 871
AluBI AGCT 4 cut(s) 496, 997, 1030, 1053
AluI AGCT 4 cut(s) 496, 997, 1030, 1053
Alw21I GWGCWC 1 cut(s) 999
AlwI GGATC 2 cut(s) 477, 637
AlwNI CAGNNNCTG 1 cut(s) 979
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 2 cut(s) 948, 1019
AspLEI GCGC 2 cut(s) 860, 929
AspS9I GGNCC 2 cut(s) 336, 402
AsuHPI GGTGA 1 cut(s) 184
AvaII GGWCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 999
BbsI GAAGAC 2 cut(s) 426, 1103
Bbv12I GWGCWC 1 cut(s) 999
BbvI GCAGC 1 cut(s) 556
BccI CCATC 4 cut(s) 109, 123, 618, 620
BcgI CGANNNNNNTGC 2 cut(s) 972, 1006
BciT130I CCWGG 1 cut(s) 980
BciVI GTATCC 2 cut(s) 446, 676
BclI TGATCA 1 cut(s) 247
BfaI CTAG 4 cut(s) 80, 705, 773, 1050
BfmI CTRYAG 2 cut(s) 697, 718
BfoI RGCGCY 1 cut(s) 930
BfuI GTATCC 2 cut(s) 446, 676
BisI GCNGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 546
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 1 cut(s) 980
Bme18I GGWCC 1 cut(s) 402
BmgT120I GGNCC 2 cut(s) 336, 402
BmiI GGNNCC 3 cut(s) 120, 135, 404
BmrFI CCNGG 1 cut(s) 980
BmsI GCATC 3 cut(s) 271, 370, 928
BpiI GAAGAC 2 cut(s) 426, 1103
Bpu10I CCTNAGC 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 489, 1097
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 2 cut(s) 364, 399
Bsc4I CCNNNNNNNGG 1 cut(s) 979
Bse1I ACTGG 1 cut(s) 973
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 1 cut(s) 980
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 3 cut(s) 208, 286, 361
BseLI CCNNNNNNNGG 1 cut(s) 979
BseMII CTCAG 1 cut(s) 1022
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 1 cut(s) 556
BshFI GGCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 999
BsiSI CCGG 4 cut(s) 365, 400, 530, 930
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 979
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 999
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 5 cut(s) 247, 482, 629, 676, 750
BspACI CCGC 2 cut(s) 161, 941
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 1 cut(s) 1023
BspEI TCCGGA 1 cut(s) 364
BspHI TCATGA 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 120, 135, 404
BspMAI CTGCAG 1 cut(s) 701
BspPI GGATC 2 cut(s) 477, 637
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 5 cut(s) 247, 482, 629, 676, 750
Bst2UI CCWGG 1 cut(s) 980
Bst4CI ACNGT 3 cut(s) 104, 196, 969
Bst6I CTCTTC 1 cut(s) 1086
BstAPI GCANNNNNTGC 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 161, 1044, 1073
BstDEI CTNAG 3 cut(s) 164, 311, 1031
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 3 cut(s) 208, 286, 361
BstH2I RGCGCY 1 cut(s) 930
BstHHI GCGC 2 cut(s) 860, 929
BstKTI GATC 5 cut(s) 250, 485, 632, 679, 753
BstMBI GATC 5 cut(s) 247, 482, 629, 676, 750
BstMWI GCNNNNNNNGC 5 cut(s) 298, 307, 544, 705, 714
BstNI CCWGG 1 cut(s) 980
BstNSI RCATGY 2 cut(s) 189, 814
BstSCI CCNGG 1 cut(s) 978
BstSFI CTRYAG 2 cut(s) 697, 718
BstV1I GCAGC 1 cut(s) 556
BstV2I GAAGAC 2 cut(s) 426, 1103
BstX2I RGATCY 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 129
BstYI RGATCY 1 cut(s) 629
BsuI GTATCC 2 cut(s) 446, 676
BsuRI GGCC 1 cut(s) 338
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 3 cut(s) 208, 286, 361
BtsIMutI CAGTG 3 cut(s) 625, 980, 1072
Cac8I GCNNGC 3 cut(s) 161, 1044, 1073
CaiI CAGNNNCTG 1 cut(s) 979
CciI TCATGA 1 cut(s) 111
CfoI GCGC 2 cut(s) 860, 929
Cfr13I GGNCC 2 cut(s) 336, 402
CseI GACGC 2 cut(s) 178, 290
Csp6I GTAC 1 cut(s) 702
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 3 cut(s) 164, 311, 1031
DpnI GATC 5 cut(s) 249, 484, 631, 678, 752
DpnII GATC 5 cut(s) 247, 482, 629, 676, 750
DraIII CACNNNGTG 2 cut(s) 358, 456
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
Ecl136II GAGCTC 1 cut(s) 997
Eco24I GRGCYC 1 cut(s) 999
Eco32I GATATC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 997
EcoICRI GAGCTC 1 cut(s) 997
EcoRII CCWGG 1 cut(s) 978
EcoRV GATATC 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 999
FaqI GGGAC 1 cut(s) 388
FauI CCCGC 1 cut(s) 168
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 545
FokI GGATG 3 cut(s) 195, 293, 348
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 1 cut(s) 545
FspBI CTAG 4 cut(s) 80, 705, 773, 1050
GlaI GCGC 2 cut(s) 859, 928
GluI GCNGC 1 cut(s) 545
HaeII RGCGCY 1 cut(s) 930
HaeIII GGCC 1 cut(s) 338
HapII CCGG 4 cut(s) 365, 400, 530, 930
HgaI GACGC 2 cut(s) 178, 290
HhaI GCGC 2 cut(s) 860, 929
Hin6I GCGC 2 cut(s) 858, 927
HinP1I GCGC 2 cut(s) 858, 927
HinfI GANTC 3 cut(s) 176, 785, 1004
HpaII CCGG 4 cut(s) 365, 400, 530, 930
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 2 cut(s) 738, 994
Hpy188III TCNNGA 5 cut(s) 112, 353, 365, 680, 782
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 5 cut(s) 367, 373, 820, 901, 1007
HpyCH4III ACNGT 3 cut(s) 104, 196, 969
HpyCH4IV ACGT 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 5 cut(s) 298, 307, 544, 705, 714
HpyF3I CTNAG 3 cut(s) 164, 311, 1031
HpySE526I ACGT 1 cut(s) 24
HspAI GCGC 2 cut(s) 858, 927
Kpn2I TCCGGA 1 cut(s) 364
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 5 cut(s) 247, 482, 629, 676, 750
LmnI GCTCC 3 cut(s) 124, 532, 994
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 3 cut(s) 271, 370, 928
MaeI CTAG 4 cut(s) 80, 705, 773, 1050
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 4 cut(s) 190, 446, 620, 914
MalI GATC 5 cut(s) 249, 484, 631, 678, 752
MboI GATC 5 cut(s) 247, 482, 629, 676, 750
MboII GAAGA 8 cut(s) 47, 100, 334, 431, 624, 956, 1103, 1108
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 1 cut(s) 999
MluCI AATT 6 cut(s) 38, 614, 948, 1019, 1037, 1108
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 2 cut(s) 229, 972
MnlI CCTC 9 cut(s) 151, 222, 235, 272, 449, 452, 800, 897, 1057
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 364
MseI TTAA 3 cut(s) 75, 606, 1111
MslI CAYNNNNRTG 2 cut(s) 871, 875
MspI CCGG 4 cut(s) 365, 400, 530, 930
MspR9I CCNGG 1 cut(s) 980
Mva1269I GAATGC 1 cut(s) 312
MvaI CCWGG 1 cut(s) 980
MwoI GCNNNNNNNGC 5 cut(s) 298, 307, 544, 705, 714
NdeII GATC 5 cut(s) 247, 482, 629, 676, 750
NlaIV GGNNCC 3 cut(s) 120, 135, 404
NmuCI GTSAC 3 cut(s) 190, 620, 914
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 189, 814
OliI CACNNNNGTG 1 cut(s) 871
PagI TCATGA 1 cut(s) 111
PciI ACATGT 1 cut(s) 810
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 785, 1004
PflFI GACNNNGTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 979
PkrI GCNGC 1 cut(s) 546
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PscI ACATGT 1 cut(s) 810
Psp124BI GAGCTC 1 cut(s) 999
Psp6I CCWGG 1 cut(s) 978
PspGI CCWGG 1 cut(s) 978
PspN4I GGNNCC 3 cut(s) 120, 135, 404
PspPI GGNCC 2 cut(s) 336, 402
PsrI GAACNNNNNNTAC 2 cut(s) 953, 985
PstI CTGCAG 1 cut(s) 701
PstNI CAGNNNCTG 1 cut(s) 979
PsuI RGATCY 1 cut(s) 629
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
RseI CAYNNNNRTG 2 cut(s) 871, 875
SacI GAGCTC 1 cut(s) 999
SaqAI TTAA 3 cut(s) 75, 606, 1111
SatI GCNGC 1 cut(s) 545
Sau3AI GATC 5 cut(s) 247, 482, 629, 676, 750
Sau96I GGNCC 2 cut(s) 336, 402
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 980
SduI GDGCHC 1 cut(s) 999
SfaNI GCATC 3 cut(s) 271, 370, 928
SfcI CTRYAG 2 cut(s) 697, 718
SinI GGWCC 1 cut(s) 402
SmiMI CAYNNNNRTG 2 cut(s) 871, 875
SmlI CTYRAG 2 cut(s) 504, 1076
SmoI CTYRAG 2 cut(s) 504, 1076
Sse9I AATT 6 cut(s) 38, 614, 948, 1019, 1037, 1108
SsiI CCGC 2 cut(s) 161, 941
SspMI CTAG 4 cut(s) 80, 705, 773, 1050
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 1 cut(s) 978
TaaI ACNGT 3 cut(s) 104, 196, 969
TaiI ACGT 1 cut(s) 27
TaqI TCGA 5 cut(s) 153, 304, 320, 391, 481
TasI AATT 6 cut(s) 38, 614, 948, 1019, 1037, 1108
TatI WGTACW 1 cut(s) 701
TfiI GAWTC 2 cut(s) 785, 1004
Tru1I TTAA 3 cut(s) 75, 606, 1111
Tru9I TTAA 3 cut(s) 75, 606, 1111
TscAI CASTG 3 cut(s) 625, 980, 1072
TseFI GTSAC 3 cut(s) 190, 620, 914
TseI GCWGC 1 cut(s) 544
Tsp45I GTSAC 3 cut(s) 190, 620, 914
TspDTI ATGAA 8 cut(s) 100, 245, 345, 624, 783, 843, 887, 996
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 3 cut(s) 625, 980, 1072
Tth111I GACNNNGTC 1 cut(s) 425
Van91I CCANNNNNTGG 1 cut(s) 979
VpaK11BI GGWCC 1 cut(s) 402
XapI RAATTY 2 cut(s) 948, 1019
XceI RCATGY 2 cut(s) 189, 814
XspI CTAG 4 cut(s) 80, 705, 773, 1050
ZrmI AGTACT 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.