MD00G1096200.v1.1

Protein DYAD-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002893
Physical Location & Seq
Reverse (-)
19991665 .. 19995166
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1096200.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGCTGGAAATTTTGAAGGCTGAAGATGAAGCTTTTGGAAACCCAATTTCCAGGAGAGATTTGAGGATGGCAGCTCGTAAGCGGATTGGTGATACTGGGCTGCTTGATCACTTACTGAAGCATCTTGATGGTAAAGTGGCACTAGGTGGGACCGAGCGGTTTCGGCGGTGGTTCAACACCAGTGGAAGAATGGAGTACTGTTTGGAGAGTGCTGACCTGGCTAACATTCGGTGGGAGGCTAGGGTGCATGATCCTTACTGGATTCCATCATCGAGGTTGATGCCAGGTGGTGGCCCCTTGGACGATTCTGTTTCTGCTGGAGAACTGAAGCTACTCAAGGCAGAAGTAGATACGATGAAGAGAATGTGTTGTGACTCGTGTCGCGACAATTTTGAGGAATTAATGAAATGGAAAACCAAAGTCAAGCAACAGGTAGTGGAAATGACAAATGTTTTGAGTGATATGCAAGGACCAAAGCAAAACACTGTCTCCAAGCCTGAAGAATCTGAGCAATGGGAGGATTGGCTAGAGAGCACCAACCTTGACAGTTTTCAGGGAAATGAACTTGTGCCATGGTTTGAGAGCAACAATCTTGTTAATTCTGAGGAAGAAGTTATAATCCAAGATCCCTATTTAGCCCTACCGCTTAGATCAAAGCATGGTGATGGTTCATCCCAGGACCTTTTTTGCAAAATCAAGGAAGAAATGGCCGAAATGGAGAGAGATGTGCTCGAGCTGGTACCGGAGAAGCAAAGGGAATATCAAACTAATTTGACCCCTGATTCTTCTGCTACTGCAAATTCAAAGTCGGATCTTGACAATCTGGTTGTGTTTCAGGAAATGTTTCAGGAGCTGTTTAATTGGAAGTCTACAACAGAGCAGACTCTGAGTGAGATATCAAATTCCATGAATGTCATGAAGCAGAATCCAAAACTATTGACTCCACCAGCCCCTCAGCTACCAGAAGATGGAAATCACTGTATGCAATATCATCCTTGTTTCATGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

38.62

Weight (kDa)

4.83

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 2 - 70 4.7e-20 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 617
Acc65I GGTACC 1 cut(s) 739
AccB1I GGYRCC 1 cut(s) 739
AccB7I CCANNNNNTGG 2 cut(s) 290, 968
AccBSI CCGCTC 1 cut(s) 157
AccI GTMKAC 1 cut(s) 869
AccII CGCG 1 cut(s) 384
AciI CCGC 4 cut(s) 82, 157, 166, 644
AclWI GGATC 3 cut(s) 245, 620, 819
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 3 cut(s) 9, 799, 901
AcuI CTGAAG 4 cut(s) 42, 137, 347, 519
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 2 cut(s) 197, 741
AfiI CCNNNNNNNGG 2 cut(s) 290, 968
AgsI TTSAA 3 cut(s) 16, 175, 804
AjnI CCWGG 4 cut(s) 50, 216, 283, 675
AluBI AGCT 6 cut(s) 32, 74, 331, 736, 853, 958
AluI AGCT 6 cut(s) 32, 74, 331, 736, 853, 958
Alw21I GWGCWC 2 cut(s) 536, 732
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 3 cut(s) 245, 620, 819
AlwNI CAGNNNCTG 2 cut(s) 853, 886
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 2 cut(s) 292, 708
ApeKI GCWGC 2 cut(s) 71, 100
ApoI RAATTY 3 cut(s) 9, 799, 901
ArsI GACNNNNNNTTYG 4 cut(s) 436, 468, 791, 823
AseI ATTAAT 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 843
Asp718I GGTACC 1 cut(s) 739
AspS9I GGNCC 4 cut(s) 150, 293, 470, 679
AsuHPI GGTGA 2 cut(s) 101, 674
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 3 cut(s) 150, 470, 679
BanI GGYRCC 1 cut(s) 739
BauI CACGAG 1 cut(s) 376
Bbv12I GWGCWC 2 cut(s) 536, 732
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 2 cut(s) 83, 87
BccI CCATC 5 cut(s) 61, 122, 274, 659, 962
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 4 cut(s) 52, 218, 285, 677
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 3 cut(s) 143, 240, 527
BisI GCNGC 2 cut(s) 72, 101
BlsI GCNGC 2 cut(s) 73, 102
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 4 cut(s) 52, 218, 285, 677
Bme18I GGWCC 3 cut(s) 150, 470, 679
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 4 cut(s) 150, 293, 470, 679
BmiI GGNNCC 3 cut(s) 151, 295, 741
BmrFI CCNGG 4 cut(s) 52, 218, 285, 677
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 2 cut(s) 130, 270
BmuI ACTGGG 1 cut(s) 105
BoxI GACNNNNGTC 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 714, 746
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 954
BpuEI CTTGAG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 972
BsaJI CCNNGG 3 cut(s) 297, 572, 675
BsaWI WCCGGW 1 cut(s) 742
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 968
Bse1I ACTGG 3 cut(s) 100, 180, 263
Bse3DI GCAATG 1 cut(s) 518
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 4 cut(s) 52, 218, 285, 677
BseDI CCNNGG 3 cut(s) 297, 572, 675
BseGI GGATG 3 cut(s) 72, 671, 991
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 2 cut(s) 290, 968
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 4 cut(s) 498, 594, 878, 968
BseNI ACTGG 3 cut(s) 100, 180, 263
BseXI GCAGC 2 cut(s) 83, 87
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 2 cut(s) 294, 710
BshNI GGYRCC 1 cut(s) 739
BsiHKAI GWGCWC 2 cut(s) 536, 732
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 1 cut(s) 743
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 2 cut(s) 290, 968
BsmAI GTCTC 1 cut(s) 493
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 2 cut(s) 294, 710
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 536, 732
Bsp143I GATC 5 cut(s) 106, 250, 625, 650, 811
Bsp19I CCATGG 1 cut(s) 572
Bsp68I TCGCGA 1 cut(s) 384
BspACI CCGC 4 cut(s) 82, 157, 166, 644
BspANI GGCC 2 cut(s) 294, 710
BspCNI CTCAG 4 cut(s) 499, 595, 879, 967
BspFNI CGCG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 3 cut(s) 151, 295, 741
BspPI GGATC 3 cut(s) 245, 620, 819
BspT107I GGYRCC 1 cut(s) 739
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 518
BsrI ACTGG 3 cut(s) 100, 180, 263
BssECI CCNNGG 3 cut(s) 297, 572, 675
BssMI GATC 5 cut(s) 106, 250, 625, 650, 811
BssSI CACGAG 1 cut(s) 376
BssT1I CCWWGG 2 cut(s) 297, 572
Bst2BI CACGAG 1 cut(s) 376
Bst2UI CCWGG 4 cut(s) 52, 218, 285, 677
Bst4CI ACNGT 4 cut(s) 200, 487, 548, 980
Bst6I CTCTTC 1 cut(s) 353
BstDEI CTNAG 5 cut(s) 507, 603, 647, 887, 954
BstDSI CCRYGG 1 cut(s) 572
BstF5I GGATG 3 cut(s) 72, 671, 991
BstFNI CGCG 1 cut(s) 384
BstKTI GATC 5 cut(s) 109, 253, 628, 653, 814
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 5 cut(s) 106, 250, 625, 650, 811
BstMWI GCNNNNNNNGC 2 cut(s) 163, 218
BstNI CCWGG 4 cut(s) 52, 218, 285, 677
BstPAI GACNNNNGTC 1 cut(s) 378
BstSCI CCNGG 4 cut(s) 50, 216, 283, 675
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 83, 87
BstX2I RGATCY 2 cut(s) 625, 811
BstYI RGATCY 2 cut(s) 625, 811
BsuRI GGCC 2 cut(s) 294, 710
BtgI CCRYGG 1 cut(s) 572
BtsCI GGATG 3 cut(s) 72, 671, 991
BtsIMutI CAGTG 3 cut(s) 187, 483, 976
BtuMI TCGCGA 1 cut(s) 384
CaiI CAGNNNCTG 2 cut(s) 853, 886
CciI TCATGA 1 cut(s) 915
Cfr13I GGNCC 4 cut(s) 150, 293, 470, 679
Csp6I GTAC 2 cut(s) 196, 740
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 6 cut(s) 248, 573, 659, 907, 916, 1003
CviQI GTAC 2 cut(s) 196, 740
DdeI CTNAG 5 cut(s) 507, 603, 647, 887, 954
DpnI GATC 5 cut(s) 108, 252, 627, 652, 813
DpnII GATC 5 cut(s) 106, 250, 625, 650, 811
DraIII CACNNNGTG 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 297, 572
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 3 cut(s) 150, 470, 679
Eco57I CTGAAG 4 cut(s) 42, 137, 347, 519
Eco88I CYCGRG 1 cut(s) 731
EcoO109I RGGNCCY 1 cut(s) 679
EcoRII CCWGG 4 cut(s) 50, 216, 283, 675
EcoRV GATATC 1 cut(s) 897
EcoT14I CCWWGG 2 cut(s) 297, 572
ErhI CCWWGG 2 cut(s) 297, 572
FaeI CATG 6 cut(s) 251, 576, 662, 910, 919, 1006
FaiI YATR 9 cut(s) 249, 464, 574, 617, 660, 908, 917, 983, 1004
FaqI GGGAC 1 cut(s) 163
FatI CATG 6 cut(s) 247, 572, 658, 906, 915, 1002
FbaI TGATCA 1 cut(s) 106
FblI GTMKAC 1 cut(s) 869
Fnu4HI GCNGC 2 cut(s) 72, 101
FokI GGATG 3 cut(s) 79, 658, 978
Fsp4HI GCNGC 2 cut(s) 72, 101
FspBI CTAG 3 cut(s) 143, 240, 527
GluI GCNGC 2 cut(s) 72, 101
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 2 cut(s) 294, 710
HapII CCGG 1 cut(s) 743
Hin1II CATG 6 cut(s) 251, 576, 662, 910, 919, 1006
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 8 cut(s) 262, 305, 374, 503, 782, 883, 925, 940
HpaII CCGG 1 cut(s) 743
HphI GGTGA 2 cut(s) 101, 674
Hpy166II GTNNAC 1 cut(s) 870
Hpy188I TCNGA 4 cut(s) 508, 604, 811, 888
Hpy188III TCNNGA 6 cut(s) 125, 383, 815, 836, 848, 916
Hpy8I GTNNAC 1 cut(s) 870
HpyAV CCTTC 1 cut(s) 10
HpyCH4III ACNGT 4 cut(s) 200, 487, 548, 980
HpyCH4V TGCA 5 cut(s) 247, 466, 690, 797, 985
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 218
HpyF3I CTNAG 5 cut(s) 507, 603, 647, 887, 954
Hsp92II CATG 6 cut(s) 251, 576, 662, 910, 919, 1006
KpnI GGTACC 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 5 cut(s) 106, 250, 625, 650, 811
LmnI GCTCC 1 cut(s) 850
Lsp1109I GCAGC 2 cut(s) 83, 87
LweI GCATC 2 cut(s) 130, 270
MaeI CTAG 3 cut(s) 143, 240, 527
MaeIII GTNAC 1 cut(s) 371
MalI GATC 5 cut(s) 108, 252, 627, 652, 813
MbiI CCGCTC 1 cut(s) 157
MboI GATC 5 cut(s) 106, 250, 625, 650, 811
MboII GAAGA 8 cut(s) 35, 198, 370, 512, 620, 713, 777, 977
MflI RGATCY 2 cut(s) 625, 811
MhlI GDGCHC 2 cut(s) 536, 732
MluCI AATT 9 cut(s) 9, 45, 388, 398, 598, 769, 799, 859, 901
MlyI GAGTC 3 cut(s) 368, 877, 934
MmeI TCCRAC 1 cut(s) 789
MnlI CCTC 7 cut(s) 57, 229, 267, 388, 511, 598, 963
MroXI GAANNNNTTC 1 cut(s) 843
MseI TTAA 3 cut(s) 401, 597, 858
MslI CAYNNNNRTG 2 cut(s) 126, 663
MspI CCGG 1 cut(s) 743
MspR9I CCNGG 4 cut(s) 52, 218, 285, 677
MvaI CCWGG 4 cut(s) 52, 218, 285, 677
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 163, 218
NcoI CCATGG 1 cut(s) 572
NdeII GATC 5 cut(s) 106, 250, 625, 650, 811
NlaIII CATG 6 cut(s) 251, 576, 662, 910, 919, 1006
NlaIV GGNNCC 3 cut(s) 151, 295, 741
NmuCI GTSAC 1 cut(s) 371
NruI TCGCGA 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 731
PagI TCATGA 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 5 cut(s) 262, 305, 503, 782, 925
PflMI CCANNNNNTGG 2 cut(s) 290, 968
PfoI TCCNGGA 1 cut(s) 50
PkrI GCNGC 2 cut(s) 73, 102
PleI GAGTC 3 cut(s) 368, 877, 934
PpsI GAGTC 3 cut(s) 368, 877, 934
PpuMI RGGWCCY 1 cut(s) 679
PshAI GACNNNNGTC 1 cut(s) 378
PshBI ATTAAT 1 cut(s) 401
PsiI TTATAA 1 cut(s) 617
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 4 cut(s) 50, 216, 283, 675
PspGI CCWGG 4 cut(s) 50, 216, 283, 675
PspN4I GGNNCC 3 cut(s) 151, 295, 741
PspPI GGNCC 4 cut(s) 150, 293, 470, 679
PspPPI RGGWCCY 1 cut(s) 679
PspXI VCTCGAGB 1 cut(s) 731
PsrI GAACNNNNNNTAC 2 cut(s) 315, 347
PstNI CAGNNNCTG 2 cut(s) 853, 886
PsuI RGATCY 2 cut(s) 625, 811
RruI TCGCGA 1 cut(s) 384
RsaI GTAC 2 cut(s) 197, 741
RsaNI GTAC 2 cut(s) 196, 740
RseI CAYNNNNRTG 2 cut(s) 126, 663
SaqAI TTAA 3 cut(s) 401, 597, 858
SatI GCNGC 2 cut(s) 72, 101
Sau3AI GATC 5 cut(s) 106, 250, 625, 650, 811
Sau96I GGNCC 4 cut(s) 150, 293, 470, 679
ScaI AGTACT 1 cut(s) 197
SchI GAGTC 3 cut(s) 368, 877, 934
ScrFI CCNGG 4 cut(s) 52, 218, 285, 677
SduI GDGCHC 2 cut(s) 536, 732
SfaNI GCATC 2 cut(s) 130, 270
Sfr274I CTCGAG 1 cut(s) 731
SinI GGWCC 3 cut(s) 150, 470, 679
SlaI CTCGAG 1 cut(s) 731
SmiMI CAYNNNNRTG 2 cut(s) 126, 663
SmlI CTYRAG 2 cut(s) 335, 731
SmoI CTYRAG 2 cut(s) 335, 731
Sse9I AATT 9 cut(s) 9, 45, 388, 398, 598, 769, 799, 859, 901
SsiI CCGC 4 cut(s) 82, 157, 166, 644
SspMI CTAG 3 cut(s) 143, 240, 527
StyD4I CCNGG 4 cut(s) 50, 216, 283, 675
StyI CCWWGG 2 cut(s) 297, 572
TaaI ACNGT 4 cut(s) 200, 487, 548, 980
TaqI TCGA 2 cut(s) 272, 732
TaqII GACCGA 1 cut(s) 167
TasI AATT 9 cut(s) 9, 45, 388, 398, 598, 769, 799, 859, 901
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 5 cut(s) 262, 305, 503, 782, 925
Tru1I TTAA 3 cut(s) 401, 597, 858
Tru9I TTAA 3 cut(s) 401, 597, 858
TscAI CASTG 3 cut(s) 187, 490, 983
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 2 cut(s) 71, 100
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 8 cut(s) 42, 371, 419, 576, 660, 923, 932, 991
TspRI CASTG 3 cut(s) 187, 490, 983
Van91I CCANNNNNTGG 2 cut(s) 290, 968
VpaK11BI GGWCC 3 cut(s) 150, 470, 679
VspI ATTAAT 1 cut(s) 401
XapI RAATTY 3 cut(s) 9, 799, 901
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XhoI CTCGAG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 869
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 3 cut(s) 143, 240, 527
ZrmI AGTACT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.