MD15G1140600.v1.1

Protein DYAD-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002893
Physical Location & Seq
Reverse (-)
10381960 .. 10385107
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1140600.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGCACAGTGGGGTACTCGTCGGCGAATTGTCTTCCTTGGAAGACACGATGGAAATAAACCTCAACTTTCATCCGGCAGCAGTTATTCAACACATGAAGTGAAAGCTGTGAAAACTGAACCCCTGGAGGTACCAGAGATCAAGAAGAGGAAGCGCCTTAGCCTCAGTGAACTGAAAGAGAAGGAAGCATTACTTTGTACGAAGAGGCCAACTAGTTCCAAGGTTATGACAAAGAAGTGTAGTAATGTGATTGAAAGATGGACAGTTGAAAGGTATAAGCAAGCAGAGCAAGCTATACTGGAAATTTTGAAGGCTGAAGATGCAACTTTTGGAAACCCAATTTCCAGGAGCGACTTGAGGATGGCAGCTCGTAAGCGGATTGGTGATACTGGGCTGCTCGATCACTTACTGAAGCACCTTGATGGTAAAGTGGCACCAGGTGGGAACGAGCGGTTTCGGCGGTGGTTCAACACCAGCGGAAGAATGGAGTACTGGTTGGAGAGTGATGACCTGGCTAATATACGGCAGGAGGCTGGGGTGCATGATCCTTACTGGATTCCAACGTCTAGGTTGATGCCAGGTGGTGGCCCCTTTGATGATTCTGTTTCTGCTGGAGAACTGAAGCTACTCAAGGCAGAAGTAGATAAAATGAAGAGTGATATACAGGAGCTGCTTTTATCCCAGAAGCAAGAGAAAGACCAAGCCAACCAGGAGAGGCTCGAGGATTTGGCAAAATGGAAAGCTCAGTCTGAGCAAAGCTTGAAAGTGATTTTAGGTTCTTGGAAGGGTATGCAGGACAATTTTGAGGAATTAATGACATGGAAGGATAAAGTCGAGCAACAGCTAGTGGAAATTACAAATGTTTTGAATAATATGCAAGGACCAAAGCAATACACTGTCACCAAGACTGAAGCATCTGAGCAATGGGAAGATTGGCTAGAGAGCACCAACCTTGACGGTTTTCAGGGAAATAAACTTGTGCCGTGGTTTGAGAGCAACAGTCTGGTTAATTCTGAGGAAGAATTTATAATCCAAGATCCCTGCTTAGGCCTACGGCTTAGATCAAAGCATGGTGATAGCTCATCTCAGGACCCTTTTTGCAAAGTCAAGGAAGATAAGGCTGAAATGGAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

43.38

Weight (kDa)

6.39

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 86 - 168 2.1e-27 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1028
Acc65I GGTACC 1 cut(s) 130
AccB1I GGYRCC 2 cut(s) 130, 433
AccB7I CCANNNNNTGG 1 cut(s) 584
AccBSI CCGCTC 1 cut(s) 451
AciI CCGC 4 cut(s) 376, 451, 460, 477
AclWI GGATC 2 cut(s) 539, 1031
AcsI RAATTY 2 cut(s) 303, 1022
AcuI CTGAAG 4 cut(s) 336, 431, 641, 930
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 4 cut(s) 16, 132, 199, 491
AfiI CCNNNNNNNGG 2 cut(s) 584, 1046
AgsI TTSAA 7 cut(s) 90, 254, 269, 310, 469, 763, 868
AhlI ACTAGT 1 cut(s) 212
AjnI CCWGG 6 cut(s) 123, 344, 436, 510, 577, 708
AluBI AGCT 9 cut(s) 107, 293, 368, 625, 670, 743, 759, 844, 1080
AluI AGCT 9 cut(s) 107, 293, 368, 625, 670, 743, 759, 844, 1080
Alw21I GWGCWC 1 cut(s) 947
AlwI GGATC 2 cut(s) 539, 1031
AlwNI CAGNNNCTG 1 cut(s) 670
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 3 cut(s) 206, 586, 1048
ApeKI GCWGC 4 cut(s) 78, 365, 394, 670
ApoI RAATTY 2 cut(s) 303, 1022
AseI ATTAAT 1 cut(s) 812
Asp718I GGTACC 1 cut(s) 130
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 3 cut(s) 587, 881, 1090
AsuHPI GGTGA 3 cut(s) 395, 892, 1085
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 2 cut(s) 881, 1090
BanI GGYRCC 2 cut(s) 130, 433
BbsI GAAGAC 2 cut(s) 25, 49
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 4 cut(s) 90, 377, 381, 657
BccI CCATC 4 cut(s) 44, 252, 355, 416
BceAI ACGGC 3 cut(s) 539, 967, 1070
BciT130I CCWGG 6 cut(s) 125, 346, 438, 512, 579, 710
BcuI ACTAGT 1 cut(s) 212
BfaI CTAG 4 cut(s) 213, 567, 845, 938
BfoI RGCGCY 1 cut(s) 157
BisI GCNGC 4 cut(s) 79, 366, 395, 671
BlsI GCNGC 4 cut(s) 80, 367, 396, 672
BmcAI AGTACT 1 cut(s) 491
Bme1390I CCNGG 6 cut(s) 125, 346, 438, 512, 579, 710
Bme18I GGWCC 2 cut(s) 881, 1090
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 3 cut(s) 587, 881, 1090
BmiI GGNNCC 4 cut(s) 132, 435, 589, 1092
BmrFI CCNGG 6 cut(s) 125, 346, 438, 512, 579, 710
BmrI ACTGGG 1 cut(s) 399
BmsI GCATC 3 cut(s) 310, 564, 923
BmuI ACTGGG 1 cut(s) 399
BpiI GAAGAC 2 cut(s) 25, 49
BpmI CTGGAG 2 cut(s) 146, 633
Bpu10I CCTNAGC 2 cut(s) 158, 1045
BpuEI CTTGAG 2 cut(s) 376, 614
BsaJI CCNNGG 4 cut(s) 37, 123, 219, 983
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 584, 1046
Bse1I ACTGG 4 cut(s) 303, 394, 497, 557
Bse3DI GCAATG 1 cut(s) 929
BseBI CCWGG 6 cut(s) 125, 346, 438, 512, 579, 710
BseDI CCNNGG 4 cut(s) 37, 123, 219, 983
BseGI GGATG 2 cut(s) 71, 366
BseLI CCNNNNNNNGG 2 cut(s) 584, 1046
BseMI GCAATG 1 cut(s) 929
BseMII CTCAG 6 cut(s) 178, 741, 758, 909, 1005, 1100
BseNI ACTGG 4 cut(s) 303, 394, 497, 557
BseXI GCAGC 4 cut(s) 90, 377, 381, 657
BseYI CCCAGC 1 cut(s) 533
BshFI GGCC 3 cut(s) 208, 588, 1050
BshNI GGYRCC 2 cut(s) 130, 433
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 584, 1046
BsnI GGCC 3 cut(s) 208, 588, 1050
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 947
Bsp143I GATC 5 cut(s) 138, 400, 544, 1036, 1061
BspACI CCGC 4 cut(s) 376, 451, 460, 477
BspANI GGCC 3 cut(s) 208, 588, 1050
BspCNI CTCAG 6 cut(s) 177, 742, 757, 910, 1006, 1099
BspLI GGNNCC 4 cut(s) 132, 435, 589, 1092
BspPI GGATC 2 cut(s) 539, 1031
BspT107I GGYRCC 2 cut(s) 130, 433
BsrBI CCGCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 929
BsrI ACTGG 4 cut(s) 303, 394, 497, 557
BssECI CCNNGG 4 cut(s) 37, 123, 219, 983
BssMI GATC 5 cut(s) 138, 400, 544, 1036, 1061
BssT1I CCWWGG 2 cut(s) 37, 219
Bst2UI CCWGG 6 cut(s) 125, 346, 438, 512, 579, 710
Bst4CI ACNGT 5 cut(s) 9, 265, 898, 959, 1001
Bst6I CTCTTC 3 cut(s) 140, 197, 647
BstC8I GCNNGC 2 cut(s) 282, 291
BstDEI CTNAG 9 cut(s) 158, 164, 744, 750, 918, 1014, 1045, 1058, 1086
BstDSI CCRYGG 1 cut(s) 983
BstF5I GGATG 2 cut(s) 71, 366
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 5 cut(s) 141, 403, 547, 1039, 1064
BstMBI GATC 5 cut(s) 138, 400, 544, 1036, 1061
BstMWI GCNNNNNNNGC 4 cut(s) 286, 290, 320, 457
BstNI CCWGG 6 cut(s) 125, 346, 438, 512, 579, 710
BstSCI CCNGG 6 cut(s) 123, 344, 436, 510, 577, 708
BstV1I GCAGC 4 cut(s) 90, 377, 381, 657
BstV2I GAAGAC 2 cut(s) 25, 49
BstX2I RGATCY 1 cut(s) 1036
BstYI RGATCY 1 cut(s) 1036
BsuRI GGCC 3 cut(s) 208, 588, 1050
BtgI CCRYGG 1 cut(s) 983
BtsCI GGATG 2 cut(s) 71, 366
BtsIMutI CAGTG 3 cut(s) 14, 172, 894
Cac8I GCNNGC 2 cut(s) 282, 291
CaiI CAGNNNCTG 1 cut(s) 670
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 3 cut(s) 587, 881, 1090
CsiI ACCWGGT 1 cut(s) 436
Csp6I GTAC 4 cut(s) 15, 131, 198, 490
CviAII CATG 4 cut(s) 95, 542, 819, 1070
CviQI GTAC 4 cut(s) 15, 131, 198, 490
DdeI CTNAG 9 cut(s) 158, 164, 744, 750, 918, 1014, 1045, 1058, 1086
DpnI GATC 5 cut(s) 140, 402, 546, 1038, 1063
DpnII GATC 5 cut(s) 138, 400, 544, 1036, 1061
DraIII CACNNNGTG 1 cut(s) 440
Eam1104I CTCTTC 3 cut(s) 140, 197, 647
EarI CTCTTC 3 cut(s) 140, 197, 647
Eco130I CCWWGG 2 cut(s) 37, 219
Eco147I AGGCCT 1 cut(s) 1050
Eco47I GGWCC 2 cut(s) 881, 1090
Eco57I CTGAAG 4 cut(s) 336, 431, 641, 930
Eco88I CYCGRG 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 1090
EcoRII CCWGG 6 cut(s) 123, 344, 436, 510, 577, 708
EcoT14I CCWWGG 2 cut(s) 37, 219
ErhI CCWWGG 2 cut(s) 37, 219
FaeI CATG 4 cut(s) 98, 545, 822, 1073
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FatI CATG 4 cut(s) 94, 541, 818, 1069
Fnu4HI GCNGC 4 cut(s) 79, 366, 395, 671
FokI GGATG 2 cut(s) 58, 373
Fsp4HI GCNGC 4 cut(s) 79, 366, 395, 671
FspBI CTAG 4 cut(s) 213, 567, 845, 938
GlaI GCGC 1 cut(s) 155
GluI GCNGC 4 cut(s) 79, 366, 395, 671
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 2 cut(s) 146, 633
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 3 cut(s) 208, 588, 1050
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 156
Hin1II CATG 4 cut(s) 98, 545, 822, 1073
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 2 cut(s) 556, 599
HpaII CCGG 1 cut(s) 75
HphI GGTGA 3 cut(s) 395, 892, 1085
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 751, 919, 1015
Hpy188III TCNNGA 2 cut(s) 142, 1088
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 4 cut(s) 175, 304, 778, 817
HpyCH4III ACNGT 5 cut(s) 9, 265, 898, 959, 1001
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 5 cut(s) 323, 541, 793, 877, 1101
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 290, 320, 457
HpyF3I CTNAG 9 cut(s) 158, 164, 744, 750, 918, 1014, 1045, 1058, 1086
HpySE526I ACGT 1 cut(s) 563
Hsp92II CATG 4 cut(s) 98, 545, 822, 1073
HspAI GCGC 1 cut(s) 154
KpnI GGTACC 1 cut(s) 134
Kzo9I GATC 5 cut(s) 138, 400, 544, 1036, 1061
LmnI GCTCC 2 cut(s) 348, 667
Lsp1109I GCAGC 4 cut(s) 90, 377, 381, 657
LweI GCATC 3 cut(s) 310, 564, 923
MabI ACCWGGT 1 cut(s) 436
MaeI CTAG 4 cut(s) 213, 567, 845, 938
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 1 cut(s) 898
MalI GATC 5 cut(s) 140, 402, 546, 1038, 1063
MbiI CCGCTC 1 cut(s) 451
MboI GATC 5 cut(s) 138, 400, 544, 1036, 1061
MflI RGATCY 1 cut(s) 1036
MhlI GDGCHC 1 cut(s) 947
MluCI AATT 8 cut(s) 27, 303, 339, 799, 809, 852, 1009, 1022
MmeI TCCRAC 2 cut(s) 477, 584
MseI TTAA 2 cut(s) 812, 1008
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 6 cut(s) 125, 346, 438, 512, 579, 710
MvaI CCWGG 6 cut(s) 125, 346, 438, 512, 579, 710
MwoI GCNNNNNNNGC 4 cut(s) 286, 290, 320, 457
NdeII GATC 5 cut(s) 138, 400, 544, 1036, 1061
NlaIII CATG 4 cut(s) 98, 545, 822, 1073
NlaIV GGNNCC 4 cut(s) 132, 435, 589, 1092
NmuCI GTSAC 1 cut(s) 898
PaeR7I CTCGAG 1 cut(s) 719
PceI AGGCCT 1 cut(s) 1050
PfeI GAWTC 2 cut(s) 556, 599
PflMI CCANNNNNTGG 1 cut(s) 584
PfoI TCCNGGA 1 cut(s) 344
PkrI GCNGC 4 cut(s) 80, 367, 396, 672
PpuMI RGGWCCY 1 cut(s) 1090
PshBI ATTAAT 1 cut(s) 812
PsiI TTATAA 1 cut(s) 1028
Psp5II RGGWCCY 1 cut(s) 1090
Psp6I CCWGG 6 cut(s) 123, 344, 436, 510, 577, 708
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 6 cut(s) 123, 344, 436, 510, 577, 708
PspN4I GGNNCC 4 cut(s) 132, 435, 589, 1092
PspPI GGNCC 3 cut(s) 587, 881, 1090
PspPPI RGGWCCY 1 cut(s) 1090
PspXI VCTCGAGB 1 cut(s) 719
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PstNI CAGNNNCTG 1 cut(s) 670
PsuI RGATCY 1 cut(s) 1036
RsaI GTAC 4 cut(s) 16, 132, 199, 491
RsaNI GTAC 4 cut(s) 15, 131, 198, 490
RseI CAYNNNNRTG 1 cut(s) 420
SaqAI TTAA 2 cut(s) 812, 1008
SatI GCNGC 4 cut(s) 79, 366, 395, 671
Sau3AI GATC 5 cut(s) 138, 400, 544, 1036, 1061
Sau96I GGNCC 3 cut(s) 587, 881, 1090
ScaI AGTACT 1 cut(s) 491
ScrFI CCNGG 6 cut(s) 125, 346, 438, 512, 579, 710
SduI GDGCHC 1 cut(s) 947
SexAI ACCWGGT 1 cut(s) 436
SfaNI GCATC 3 cut(s) 310, 564, 923
Sfr274I CTCGAG 1 cut(s) 719
SinI GGWCC 2 cut(s) 881, 1090
SlaI CTCGAG 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 3 cut(s) 355, 629, 719
SmoI CTYRAG 3 cut(s) 355, 629, 719
SpeI ACTAGT 1 cut(s) 212
Sse9I AATT 8 cut(s) 27, 303, 339, 799, 809, 852, 1009, 1022
SseBI AGGCCT 1 cut(s) 1050
SsiI CCGC 4 cut(s) 376, 451, 460, 477
SspMI CTAG 4 cut(s) 213, 567, 845, 938
StuI AGGCCT 1 cut(s) 1050
StyD4I CCNGG 6 cut(s) 123, 344, 436, 510, 577, 708
StyI CCWWGG 2 cut(s) 37, 219
TaaI ACNGT 5 cut(s) 9, 265, 898, 959, 1001
TaiI ACGT 1 cut(s) 566
TaqI TCGA 3 cut(s) 399, 720, 834
TasI AATT 8 cut(s) 27, 303, 339, 799, 809, 852, 1009, 1022
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 2 cut(s) 556, 599
Tru1I TTAA 2 cut(s) 812, 1008
Tru9I TTAA 2 cut(s) 812, 1008
TscAI CASTG 3 cut(s) 14, 172, 901
TseFI GTSAC 1 cut(s) 898
TseI GCWGC 4 cut(s) 78, 365, 394, 670
Tsp45I GTSAC 1 cut(s) 898
TspDTI ATGAA 3 cut(s) 60, 111, 665
TspRI CASTG 3 cut(s) 14, 172, 901
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 881, 1090
VspI ATTAAT 1 cut(s) 812
XapI RAATTY 2 cut(s) 303, 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 481
XhoI CTCGAG 1 cut(s) 719
XspI CTAG 4 cut(s) 213, 567, 845, 938
ZrmI AGTACT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.