Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009480

Physical Location & Seq

Genomic Coordinates Reverse (-)

22457633 .. 22467925

Gene Structure 10293 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1107200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGGCTACCTTCTTCCAGAGGCTTTCCAAGGCGGCGCCGATCGCCTTCAGCAGCTCGTTCCGAGGCCAATCGAAGTCCAGCTTTCGTATTCCTTTCGGAGCTGTCGCAGCCGTCTCCGGCGGAATCTCCTACCTCTGGTACTATTCCTCGCCGGATTTGGCTTATCTAGACGAAGACAAAGAACAAGCTGGCCCAAAAGTTGCACTTAATCCAGACAAGTGGATTGAATTTAAGTTGCAAGATACTGCAAGGGTTAGCCACAATACACAATTATTCAGGTTTACATTTGATCCAACTGTTAAGTTGGGTTTAGATGTTGCTTCATGTCTCATTACGAGGGCTCCATTAGGACAAGATGCTGAAGGGAAACCAAAATATGTCATACGCCCGTATACTCCTATTTCAGATCCAGATTCCAAGGGATACTTTGACTTGTTAATTAAGGTGTATCCAGAAGGAAAAATGAGCCAACATTTTGCAAGCTTAAAACCAGGCGATGTAGTTGAAGTGAAGGGGCCCATTGAAAAACTTAGATATACTCCCAACATGAAGAAACATATAGGCATGATTGCTGGTGGTTCGGGTATTACTCCAATGCTTCAGGTTATTGAGGCCATACTGAAAAATCCTGATGACACGACTCAGGTGTCACTTCTTTATGCAAACGTCTCCCCAGATGACATACTGCTTAAACAGAAACTTGACATACTAGCAGCTAGCCACCCAAATCTAAAGGTATTTTACACGGTAGATAATCCAACAAAGAGTTGGAAAGGGGGAAAAGGTTACATATCAAAGGACATTGCAGTTAAAGGCTTACCTGCTCCTGGTGAAGATACTCTTGTCCTGGTATGCGGTCCTCCTGGGATGATGAAACATATATCTGGTGACAAGGCTAAAGACTGGTCTCAAGGAGAGGTCACTGGCATACTAAAAGAACTTGGATACACTGAAGAAATGGTTTACAAATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004128 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005758 GO:0005794 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009505 GO:0009628 GO:0009651 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0030312 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0055114 GO:0070013 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

FAD_binding_6 NAD_binding_1

Protein Analysis

325

Amino Acids

35.62

Weight (kDa)

8.74

Isoelectric Point (pI)

33.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_6	PF00970	77 - 179	2.5e-25	Oxidoreductase FAD-binding domain
NAD_binding_1	PF00175	189 - 294	1.5e-30	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20080
fragaria_vesca	FvH4_1g03580
malus_domestica	MD00G1107200.v1.1 MD02G1035100.v1.1
prunus_persica	Prupe.7G239800_v2.0.a1
pyrus_communis	pycom02g02870 pycom02g02890 pycom02g02910 pycom15g15760 pycom810g00010 pycom810g00030
rosa_chinensis	RchiOBHm_Chr2g0089111
rosa_laevigata	RLG00000015992
rosa_multiflora	Rmu_sc0003668.1_g000001 Rmu_sc0005510.1_g000001
rosa_roxburghii	Rroxscaffold_2G00152240
rosa_rugosa	Rorug01G0487500
rosa_samantha	Rh2AG043000 Rh2BG041500 Rh2CG043100 Rh2DG042100
rosa_wichuraiana	Rw2G003460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	646
Acc36I	ACCTGC	1 cut(s)	829
AccB1I	GGYRCC	1 cut(s)	34
AccI	GTMKAC	1 cut(s)	392
AciI	CCGC	3 cut(s)	32, 120, 855
AclWI	GGATC	2 cut(s)	284, 401
AcsI	RAATTY	2 cut(s)	227, 968
AcuI	CTGAAG	4 cut(s)	31, 381, 584, 972
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	1 cut(s)	140
AfiI	CCNNNNNNNGG	2 cut(s)	135, 827
AgsI	TTSAA	3 cut(s)	227, 506, 524
AjnI	CCWGG	4 cut(s)	490, 826, 846, 862
AluBI	AGCT	6 cut(s)	54, 81, 101, 188, 483, 716
AluI	AGCT	6 cut(s)	54, 81, 101, 188, 483, 716
Alw26I	GTCTC	4 cut(s)	118, 332, 673, 912
AlwI	GGATC	2 cut(s)	284, 401
AoxI	GGCC	4 cut(s)	64, 190, 515, 612
ApaI	GGGCCC	1 cut(s)	519
ApeKI	GCWGC	3 cut(s)	51, 107, 713
ApoI	RAATTY	2 cut(s)	227, 968
AspLEI	GCGC	1 cut(s)	37
AspS9I	GGNCC	4 cut(s)	191, 515, 516, 857
AsuHPI	GGTGA	2 cut(s)	842, 899
AsuNHI	GCTAGC	1 cut(s)	716
AvaII	GGWCC	1 cut(s)	857
BaeGI	GKGCMC	1 cut(s)	519
BaeI	ACNNNNGTAYC	2 cut(s)	828, 861
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	343, 519
BbsI	GAAGAC	1 cut(s)	180
BbvI	GCAGC	3 cut(s)	63, 119, 725
BceAI	ACGGC	1 cut(s)	95
BciT130I	CCWGG	4 cut(s)	492, 828, 848, 864
BciVI	GTATCC	3 cut(s)	416, 459, 938
BcoDI	GTCTC	4 cut(s)	118, 332, 673, 912
BfaI	CTAG	3 cut(s)	167, 710, 717
BfoI	RGCGCY	1 cut(s)	38
BfuAI	ACCTGC	1 cut(s)	829
BfuI	GTATCC	3 cut(s)	416, 459, 938
BisI	GCNGC	4 cut(s)	33, 52, 108, 714
BlsI	GCNGC	4 cut(s)	34, 53, 109, 715
Bme1390I	CCNGG	4 cut(s)	492, 828, 848, 864
Bme18I	GGWCC	1 cut(s)	857
BmgT120I	GGNCC	4 cut(s)	191, 515, 516, 857
BmiI	GGNNCC	4 cut(s)	36, 342, 516, 517
BmrFI	CCNGG	4 cut(s)	492, 828, 848, 864
BmsI	GCATC	1 cut(s)	346
BmtI	GCTAGC	1 cut(s)	720
BpiI	GAAGAC	1 cut(s)	180
BpuEI	CTTGAG	1 cut(s)	894
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	1 cut(s)	912
BsaJI	CCNNGG	4 cut(s)	27, 61, 417, 863
BsaXI	ACNNNNNCTCC	2 cut(s)	325, 355
Bsc4I	CCNNNNNNNGG	2 cut(s)	135, 827
Bse1I	ACTGG	2 cut(s)	908, 928
Bse3DI	GCAATG	1 cut(s)	801
BseBI	CCWGG	4 cut(s)	492, 828, 848, 864
BseDI	CCNNGG	4 cut(s)	27, 61, 417, 863
BseGI	GGATG	1 cut(s)	873
BseLI	CCNNNNNNNGG	2 cut(s)	135, 827
BseMI	GCAATG	1 cut(s)	801
BseMII	CTCAG	1 cut(s)	656
BseNI	ACTGG	2 cut(s)	908, 928
BseSI	GKGCMC	1 cut(s)	519
BseXI	GCAGC	3 cut(s)	63, 119, 725
Bsh1285I	CGRYCG	1 cut(s)	42
BshFI	GGCC	4 cut(s)	66, 192, 517, 614
BshNI	GGYRCC	1 cut(s)	34
BsiEI	CGRYCG	1 cut(s)	42
BsiSI	CCGG	2 cut(s)	117, 152
BslI	CCNNNNNNNGG	2 cut(s)	135, 827
BsmAI	GTCTC	4 cut(s)	118, 332, 673, 912
BsmBI	CGTCTC	2 cut(s)	118, 673
BsnI	GGCC	4 cut(s)	66, 192, 517, 614
Bso31I	GGTCTC	1 cut(s)	912
Bsp120I	GGGCCC	1 cut(s)	515
Bsp1286I	GDGCHC	2 cut(s)	343, 519
Bsp143I	GATC	3 cut(s)	39, 289, 406
BspACI	CCGC	3 cut(s)	32, 120, 855
BspANI	GGCC	4 cut(s)	66, 192, 517, 614
BspCNI	CTCAG	1 cut(s)	655
BspLI	GGNNCC	4 cut(s)	36, 342, 516, 517
BspMI	ACCTGC	1 cut(s)	829
BspOI	GCTAGC	1 cut(s)	720
BspPI	GGATC	2 cut(s)	284, 401
BspT107I	GGYRCC	1 cut(s)	34
BspTNI	GGTCTC	1 cut(s)	912
BsrDI	GCAATG	1 cut(s)	801
BsrI	ACTGG	2 cut(s)	908, 928
BssECI	CCNNGG	4 cut(s)	27, 61, 417, 863
BssMI	GATC	3 cut(s)	39, 289, 406
BssNAI	GTATAC	1 cut(s)	393
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	2 cut(s)	27, 417
Bst1107I	GTATAC	1 cut(s)	393
Bst2UI	CCWGG	4 cut(s)	492, 828, 848, 864
Bst4CI	ACNGT	2 cut(s)	298, 748
BstACI	GRCGYC	1 cut(s)	35
BstC8I	GCNNGC	3 cut(s)	190, 481, 718
BstDEI	CTNAG	2 cut(s)	530, 642
BstF5I	GGATG	1 cut(s)	873
BstH2I	RGCGCY	1 cut(s)	38
BstHHI	GCGC	1 cut(s)	37
BstKTI	GATC	3 cut(s)	42, 292, 409
BstMAI	GTCTC	4 cut(s)	118, 332, 673, 912
BstMBI	GATC	3 cut(s)	39, 289, 406
BstMCI	CGRYCG	1 cut(s)	42
BstMWI	GCNNNNNNNGC	2 cut(s)	41, 107
BstNI	CCWGG	4 cut(s)	492, 828, 848, 864
BstSCI	CCNGG	4 cut(s)	490, 826, 846, 862
BstSLI	GKGCMC	1 cut(s)	519
BstV1I	GCAGC	3 cut(s)	63, 119, 725
BstV2I	GAAGAC	1 cut(s)	180
BstX2I	RGATCY	1 cut(s)	406
BstXI	CCANNNNNNTGG	1 cut(s)	219
BstYI	RGATCY	1 cut(s)	406
BstZ17I	GTATAC	1 cut(s)	393
BsuI	GTATCC	3 cut(s)	416, 459, 938
BsuRI	GGCC	4 cut(s)	66, 192, 517, 614
BtgZI	GCGATG	1 cut(s)	510
BtsCI	GGATG	1 cut(s)	873
BtsIMutI	CAGTG	2 cut(s)	921, 948
BveI	ACCTGC	1 cut(s)	829
Cac8I	GCNNGC	3 cut(s)	190, 481, 718
CfoI	GCGC	1 cut(s)	37
Cfr13I	GGNCC	4 cut(s)	191, 515, 516, 857
Csp6I	GTAC	1 cut(s)	139
CviAII	CATG	3 cut(s)	324, 547, 565
CviQI	GTAC	1 cut(s)	139
DdeI	CTNAG	2 cut(s)	530, 642
DinI	GGCGCC	1 cut(s)	36
DpnI	GATC	3 cut(s)	41, 291, 408
DpnII	GATC	3 cut(s)	39, 289, 406
DrdI	GACNNNNNNGTC	1 cut(s)	646
DseDI	GACNNNNNNGTC	1 cut(s)	646
EciI	GGCGGA	1 cut(s)	135
Eco130I	CCWWGG	2 cut(s)	27, 417
Eco24I	GRGCYC	2 cut(s)	343, 519
Eco31I	GGTCTC	1 cut(s)	912
Eco47I	GGWCC	1 cut(s)	857
Eco57I	CTGAAG	4 cut(s)	31, 381, 584, 972
EcoO109I	RGGNCCY	1 cut(s)	515
EcoRII	CCWGG	4 cut(s)	490, 826, 846, 862
EcoT14I	CCWWGG	2 cut(s)	27, 417
EcoT38I	GRGCYC	2 cut(s)	343, 519
EgeI	GGCGCC	1 cut(s)	36
EheI	GGCGCC	1 cut(s)	36
ErhI	CCWWGG	2 cut(s)	27, 417
Esp3I	CGTCTC	2 cut(s)	118, 673
FaeI	CATG	3 cut(s)	327, 550, 568
FalI	AAGNNNNNCTT	8 cut(s)	65, 97, 189, 221, 410, 442, 825, 857
FatI	CATG	3 cut(s)	323, 546, 564
FblI	GTMKAC	1 cut(s)	392
Fnu4HI	GCNGC	4 cut(s)	33, 52, 108, 714
FokI	GGATG	1 cut(s)	880
FriOI	GRGCYC	2 cut(s)	343, 519
Fsp4HI	GCNGC	4 cut(s)	33, 52, 108, 714
FspBI	CTAG	3 cut(s)	167, 710, 717
GlaI	GCGC	1 cut(s)	36
GluI	GCNGC	4 cut(s)	33, 52, 108, 714
HaeII	RGCGCY	1 cut(s)	38
HaeIII	GGCC	4 cut(s)	66, 192, 517, 614
HapII	CCGG	2 cut(s)	117, 152
HhaI	GCGC	1 cut(s)	37
Hin1I	GRCGYC	1 cut(s)	35
Hin1II	CATG	3 cut(s)	327, 550, 568
Hin6I	GCGC	1 cut(s)	35
HinP1I	GCGC	1 cut(s)	35
HindIII	AAGCTT	1 cut(s)	481
HinfI	GANTC	3 cut(s)	123, 413, 640
HpaII	CCGG	2 cut(s)	117, 152
HphI	GGTGA	2 cut(s)	842, 899
Hpy166II	GTNNAC	3 cut(s)	282, 393, 964
Hpy188I	TCNGA	3 cut(s)	62, 98, 406
Hpy188III	TCNNGA	6 cut(s)	16, 167, 212, 410, 452, 629
Hpy8I	GTNNAC	3 cut(s)	282, 393, 964
HpyAV	CCTTC	5 cut(s)	19, 55, 356, 449, 505
HpyCH4III	ACNGT	2 cut(s)	298, 748
HpyCH4IV	ACGT	1 cut(s)	666
HpyCH4V	TGCA	6 cut(s)	203, 238, 248, 479, 662, 806
HpyF10VI	GCNNNNNNNGC	2 cut(s)	41, 107
HpyF3I	CTNAG	2 cut(s)	530, 642
HpySE526I	ACGT	1 cut(s)	666
Hsp92I	GRCGYC	1 cut(s)	35
Hsp92II	CATG	3 cut(s)	327, 550, 568
HspAI	GCGC	1 cut(s)	35
KasI	GGCGCC	1 cut(s)	34
Kzo9I	GATC	3 cut(s)	39, 289, 406
LmnI	GCTCC	3 cut(s)	98, 346, 829
Lsp1109I	GCAGC	3 cut(s)	63, 119, 725
LweI	GCATC	1 cut(s)	346
MaeI	CTAG	3 cut(s)	167, 710, 717
MaeII	ACGT	1 cut(s)	666
MaeIII	GTNAC	4 cut(s)	648, 785, 887, 919
MalI	GATC	3 cut(s)	41, 291, 408
MboI	GATC	3 cut(s)	39, 289, 406
MboII	GAAGA	5 cut(s)	4, 185, 562, 845, 965
MflI	RGATCY	1 cut(s)	406
MhlI	GDGCHC	2 cut(s)	343, 519
MluCI	AATT	4 cut(s)	227, 269, 438, 968
Mly113I	GGCGCC	1 cut(s)	35
MlyI	GAGTC	1 cut(s)	634
MmeI	TCCRAC	3 cut(s)	317, 749, 782
MnlI	CCTC	8 cut(s)	12, 56, 143, 157, 330, 604, 870, 910
MseI	TTAA	8 cut(s)	207, 231, 300, 437, 441, 485, 690, 810
MspI	CCGG	2 cut(s)	117, 152
MspR9I	CCNGG	4 cut(s)	492, 828, 848, 864
MvaI	CCWGG	4 cut(s)	492, 828, 848, 864
MwoI	GCNNNNNNNGC	2 cut(s)	41, 107
NarI	GGCGCC	1 cut(s)	35
NdeII	GATC	3 cut(s)	39, 289, 406
NheI	GCTAGC	1 cut(s)	716
NlaIII	CATG	3 cut(s)	327, 550, 568
NlaIV	GGNNCC	4 cut(s)	36, 342, 516, 517
NmuCI	GTSAC	3 cut(s)	648, 887, 919
PacI	TTAATTAA	1 cut(s)	441
PfeI	GAWTC	2 cut(s)	123, 413
PkrI	GCNGC	4 cut(s)	34, 53, 109, 715
Ple19I	CGATCG	1 cut(s)	42
PleI	GAGTC	1 cut(s)	634
PluTI	GGCGCC	1 cut(s)	38
PpsI	GAGTC	1 cut(s)	634
Psp6I	CCWGG	4 cut(s)	490, 826, 846, 862
PspGI	CCWGG	4 cut(s)	490, 826, 846, 862
PspN4I	GGNNCC	4 cut(s)	36, 342, 516, 517
PspOMI	GGGCCC	1 cut(s)	515
PspPI	GGNCC	4 cut(s)	191, 515, 516, 857
PsuI	RGATCY	1 cut(s)	406
PvuI	CGATCG	1 cut(s)	42
RsaI	GTAC	1 cut(s)	140
RsaNI	GTAC	1 cut(s)	139
SaqAI	TTAA	8 cut(s)	207, 231, 300, 437, 441, 485, 690, 810
SatI	GCNGC	4 cut(s)	33, 52, 108, 714
Sau3AI	GATC	3 cut(s)	39, 289, 406
Sau96I	GGNCC	4 cut(s)	191, 515, 516, 857
SchI	GAGTC	1 cut(s)	634
ScrFI	CCNGG	4 cut(s)	492, 828, 848, 864
SduI	GDGCHC	2 cut(s)	343, 519
SfaNI	GCATC	1 cut(s)	346
SfoI	GGCGCC	1 cut(s)	36
SinI	GGWCC	1 cut(s)	857
SmlI	CTYRAG	1 cut(s)	909
SmoI	CTYRAG	1 cut(s)	909
Sse9I	AATT	4 cut(s)	227, 269, 438, 968
SsiI	CCGC	3 cut(s)	32, 120, 855
SspDI	GGCGCC	1 cut(s)	34
SspMI	CTAG	3 cut(s)	167, 710, 717
StyD4I	CCNGG	4 cut(s)	490, 826, 846, 862
StyI	CCWWGG	2 cut(s)	27, 417
TaaI	ACNGT	2 cut(s)	298, 748
TaiI	ACGT	1 cut(s)	669
TaqI	TCGA	1 cut(s)	71
TasI	AATT	4 cut(s)	227, 269, 438, 968
TauI	GCSGC	1 cut(s)	35
TfiI	GAWTC	2 cut(s)	123, 413
Tru1I	TTAA	8 cut(s)	207, 231, 300, 437, 441, 485, 690, 810
Tru9I	TTAA	8 cut(s)	207, 231, 300, 437, 441, 485, 690, 810
TscAI	CASTG	2 cut(s)	928, 955
TseFI	GTSAC	3 cut(s)	648, 887, 919
TseI	GCWGC	3 cut(s)	51, 107, 713
Tsp45I	GTSAC	3 cut(s)	648, 887, 919
TspDTI	ATGAA	3 cut(s)	312, 563, 887
TspRI	CASTG	2 cut(s)	928, 955
VpaK11BI	GGWCC	1 cut(s)	857
XapI	RAATTY	2 cut(s)	227, 968
XbaI	TCTAGA	1 cut(s)	166
XcmI	CCANNNNNNNNNTGG	1 cut(s)	765
XmiI	GTMKAC	1 cut(s)	392
XspI	CTAG	3 cut(s)	167, 710, 717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.