Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009480

Physical Location & Seq

Genomic Coordinates Reverse (-)

2681238 .. 2685028

Gene Structure 3791 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1035100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGGCTACCTTCTTCCAGAGGCTCGCCAAGGCGGCGCCGACCGCCTTCAACAGCTCGTTTCGAGGCCAATCGAAGTCAAGCTTTCGCATTCCCTTCGGAGCAGTCGCAGCCGTCTCCGGCGGAATCTCCTATCTCTGCTACTATTCCTCGCCGGATTTGGTTTATCTAGATGAAATCAAAGAACAGACTGGCCCAAAAGTTGCACTTAATCCAGACAAATGGGTTGAATTTAAGCTGCAAGATACTGCAAGGGTTAGCCATAATACACAATTATTCAGGTTTACATTTGATCCGAATGCCAAGTTGGGTTTATACGTTGCTTCATGTCTCCTTACGAGGGCTCCATTAGGACAGGATGCGGAAGGGAAACCCAAATATGTCGTACGCCCGTATACTCCTATTTCCGATCCAGATGCCAAGGGATACTTTGACTTGTTAATTAAGGTGTATCCAGAAGGAAAAATGAGCCAGCATTTCGCAAGCTTAAAACCAGGCGATGTAGTTGAAGTGAAGGGGCCCATTGAAAAACTCAGATATACTCCCAACATGAAGAAACATATAGGCATGATTGCCGGTGGTACAGGTATTACTCCAATGCTTCAGGTTATTGAGGCCATACTGAAAAATCCTGATGACACGACTCAGGTGTCACTGCTTTATGCGAATGTCTCCCCAGATGATATACTGCTTAAACAGAAACTTGACATACTTGCAGCTAGCCACCCAAATTTAAAGGTATTTTACACTGTAGATAATCCAACAAAGAGTTGGAAAGGGGGAAAAGGTTACGTATCAAAGGACATGGCAGTTAAAGGCTTACCTGCTCCTGGTGAAGATACTCTCATCCTGGTATGTGGTCCTCCTGGGATGATGAAACATATATCTGGTGACAAGGCTCAAGACCGATCACAAGGAGAGCTCACTGGCATACTAAAAGAACTCGGGTACACTGAAGAAATGGTTTACAAATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004128 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005758 GO:0005794 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009505 GO:0009628 GO:0009651 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0030312 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0055114 GO:0070013 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

FAD_binding_6 NAD_binding_1

Protein Analysis

325

Amino Acids

35.59

Weight (kDa)

8.94

Isoelectric Point (pI)

32.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_6	PF00970	77 - 179	6e-26	Oxidoreductase FAD-binding domain
NAD_binding_1	PF00175	189 - 294	8.6e-32	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20080
fragaria_vesca	FvH4_1g03580
malus_domestica	MD00G1107200.v1.1 MD02G1035100.v1.1
prunus_persica	Prupe.7G239800_v2.0.a1
pyrus_communis	pycom02g02870 pycom02g02890 pycom02g02910 pycom15g15760 pycom810g00010 pycom810g00030
rosa_chinensis	RchiOBHm_Chr2g0089111
rosa_laevigata	RLG00000015992
rosa_multiflora	Rmu_sc0003668.1_g000001 Rmu_sc0005510.1_g000001
rosa_roxburghii	Rroxscaffold_2G00152240
rosa_rugosa	Rorug01G0487500
rosa_samantha	Rh2AG043000 Rh2BG041500 Rh2CG043100 Rh2DG042100
rosa_wichuraiana	Rw2G003460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	646
Acc36I	ACCTGC	1 cut(s)	829
AccB1I	GGYRCC	1 cut(s)	34
AccI	GTMKAC	1 cut(s)	392
AciI	CCGC	4 cut(s)	32, 42, 120, 359
AclWI	GGATC	2 cut(s)	284, 401
AcsI	RAATTY	3 cut(s)	227, 727, 968
AcuI	CTGAAG	2 cut(s)	584, 972
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	3 cut(s)	384, 580, 947
AfiI	CCNNNNNNNGG	2 cut(s)	337, 827
AgsI	TTSAA	4 cut(s)	49, 227, 506, 524
AjnI	CCWGG	4 cut(s)	490, 826, 846, 862
AjuI	GAANNNNNNNTTGG	2 cut(s)	287, 319
AluBI	AGCT	6 cut(s)	54, 81, 235, 483, 716, 919
AluI	AGCT	6 cut(s)	54, 81, 235, 483, 716, 919
Alw21I	GWGCWC	1 cut(s)	921
Alw26I	GTCTC	3 cut(s)	118, 332, 673
AlwI	GGATC	2 cut(s)	284, 401
Ama87I	CYCGRG	1 cut(s)	941
AoxI	GGCC	4 cut(s)	64, 190, 515, 612
ApaI	GGGCCC	1 cut(s)	519
ApeKI	GCWGC	3 cut(s)	107, 235, 713
ApoI	RAATTY	3 cut(s)	227, 727, 968
AspLEI	GCGC	1 cut(s)	37
AspS9I	GGNCC	4 cut(s)	191, 515, 516, 857
AsuHPI	GGTGA	2 cut(s)	842, 899
AsuNHI	GCTAGC	1 cut(s)	716
AvaI	CYCGRG	1 cut(s)	941
AvaII	GGWCC	1 cut(s)	857
BaeGI	GKGCMC	1 cut(s)	519
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	3 cut(s)	343, 519, 921
Bbv12I	GWGCWC	1 cut(s)	921
BbvI	GCAGC	3 cut(s)	119, 222, 725
BceAI	ACGGC	1 cut(s)	95
BcgI	CGANNNNNNTGC	4 cut(s)	76, 110, 395, 429
BciT130I	CCWGG	4 cut(s)	492, 828, 848, 864
BciVI	GTATCC	2 cut(s)	416, 459
BcoDI	GTCTC	3 cut(s)	118, 332, 673
BfaI	CTAG	2 cut(s)	167, 717
BfmI	CTRYAG	1 cut(s)	747
BfoI	RGCGCY	1 cut(s)	38
BfuAI	ACCTGC	1 cut(s)	829
BfuI	GTATCC	2 cut(s)	416, 459
BglI	GCCNNNNNGGC	1 cut(s)	32
BisI	GCNGC	4 cut(s)	33, 108, 236, 714
BlsI	GCNGC	4 cut(s)	34, 109, 237, 715
Bme1390I	CCNGG	4 cut(s)	492, 828, 848, 864
Bme18I	GGWCC	1 cut(s)	857
BmeT110I	CYCGRG	1 cut(s)	941
BmgT120I	GGNCC	4 cut(s)	191, 515, 516, 857
BmiI	GGNNCC	4 cut(s)	36, 342, 516, 517
BmrFI	CCNGG	4 cut(s)	492, 828, 848, 864
BmsI	GCATC	2 cut(s)	346, 403
BmtI	GCTAGC	1 cut(s)	720
BpuEI	CTTGAG	1 cut(s)	882
BsaAI	YACGTR	1 cut(s)	790
BsaHI	GRCGYC	1 cut(s)	35
BsaJI	CCNNGG	3 cut(s)	27, 417, 863
BsaXI	ACNNNNNCTCC	2 cut(s)	325, 355
Bsc4I	CCNNNNNNNGG	2 cut(s)	337, 827
Bse118I	RCCGGY	1 cut(s)	572
Bse1I	ACTGG	2 cut(s)	193, 928
BseBI	CCWGG	4 cut(s)	492, 828, 848, 864
BseDI	CCNNGG	3 cut(s)	27, 417, 863
BseGI	GGATG	3 cut(s)	361, 843, 873
BseLI	CCNNNNNNNGG	2 cut(s)	337, 827
BseMII	CTCAG	2 cut(s)	544, 656
BseNI	ACTGG	2 cut(s)	193, 928
BseSI	GKGCMC	1 cut(s)	519
BseXI	GCAGC	3 cut(s)	119, 222, 725
Bsh1285I	CGRYCG	1 cut(s)	42
BshFI	GGCC	4 cut(s)	66, 192, 517, 614
BshNI	GGYRCC	1 cut(s)	34
BsiEI	CGRYCG	1 cut(s)	42
BsiHKAI	GWGCWC	1 cut(s)	921
BsiHKCI	CYCGRG	1 cut(s)	941
BsiSI	CCGG	3 cut(s)	117, 152, 573
BsiWI	CGTACG	1 cut(s)	382
BslI	CCNNNNNNNGG	2 cut(s)	337, 827
BsmAI	GTCTC	3 cut(s)	118, 332, 673
BsmBI	CGTCTC	1 cut(s)	118
BsmI	GAATGC	2 cut(s)	87, 301
BsnI	GGCC	4 cut(s)	66, 192, 517, 614
BsoBI	CYCGRG	1 cut(s)	941
Bsp120I	GGGCCC	1 cut(s)	515
Bsp1286I	GDGCHC	3 cut(s)	343, 519, 921
Bsp143I	GATC	3 cut(s)	289, 406, 905
BspACI	CCGC	4 cut(s)	32, 42, 120, 359
BspANI	GGCC	4 cut(s)	66, 192, 517, 614
BspCNI	CTCAG	2 cut(s)	543, 655
BspLI	GGNNCC	4 cut(s)	36, 342, 516, 517
BspMI	ACCTGC	1 cut(s)	829
BspOI	GCTAGC	1 cut(s)	720
BspPI	GGATC	2 cut(s)	284, 401
BspT107I	GGYRCC	1 cut(s)	34
BsrFI	RCCGGY	1 cut(s)	572
BsrI	ACTGG	2 cut(s)	193, 928
BssAI	RCCGGY	1 cut(s)	572
BssECI	CCNNGG	3 cut(s)	27, 417, 863
BssMI	GATC	3 cut(s)	289, 406, 905
BssNAI	GTATAC	1 cut(s)	393
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	2 cut(s)	27, 417
Bst1107I	GTATAC	1 cut(s)	393
Bst2UI	CCWGG	4 cut(s)	492, 828, 848, 864
Bst4CI	ACNGT	1 cut(s)	748
BstACI	GRCGYC	1 cut(s)	35
BstBAI	YACGTR	1 cut(s)	790
BstC8I	GCNNGC	4 cut(s)	24, 470, 481, 718
BstDEI	CTNAG	2 cut(s)	530, 642
BstF5I	GGATG	3 cut(s)	361, 843, 873
BstH2I	RGCGCY	1 cut(s)	38
BstHHI	GCGC	1 cut(s)	37
BstKTI	GATC	3 cut(s)	292, 409, 908
BstMAI	GTCTC	3 cut(s)	118, 332, 673
BstMBI	GATC	3 cut(s)	289, 406, 905
BstMCI	CGRYCG	1 cut(s)	42
BstMWI	GCNNNNNNNGC	3 cut(s)	32, 41, 107
BstNI	CCWGG	4 cut(s)	492, 828, 848, 864
BstSCI	CCNGG	4 cut(s)	490, 826, 846, 862
BstSFI	CTRYAG	1 cut(s)	747
BstSLI	GKGCMC	1 cut(s)	519
BstSNI	TACGTA	1 cut(s)	790
BstV1I	GCAGC	3 cut(s)	119, 222, 725
BstXI	CCANNNNNNTGG	1 cut(s)	219
BstZ17I	GTATAC	1 cut(s)	393
BsuI	GTATCC	2 cut(s)	416, 459
BsuRI	GGCC	4 cut(s)	66, 192, 517, 614
BtgZI	GCGATG	1 cut(s)	510
BtsCI	GGATG	3 cut(s)	361, 843, 873
BtsI	GCAGTG	1 cut(s)	650
BtsIMutI	CAGTG	4 cut(s)	650, 744, 921, 948
BveI	ACCTGC	1 cut(s)	829
Cac8I	GCNNGC	4 cut(s)	24, 470, 481, 718
CfoI	GCGC	1 cut(s)	37
Cfr10I	RCCGGY	1 cut(s)	572
Cfr13I	GGNCC	4 cut(s)	191, 515, 516, 857
Csp6I	GTAC	3 cut(s)	383, 579, 946
CviAII	CATG	4 cut(s)	324, 547, 565, 802
CviQI	GTAC	3 cut(s)	383, 579, 946
DdeI	CTNAG	2 cut(s)	530, 642
DinI	GGCGCC	1 cut(s)	36
DpnI	GATC	3 cut(s)	291, 408, 907
DpnII	GATC	3 cut(s)	289, 406, 905
DraI	TTTAAA	1 cut(s)	732
DrdI	GACNNNNNNGTC	1 cut(s)	646
DseDI	GACNNNNNNGTC	1 cut(s)	646
EciI	GGCGGA	1 cut(s)	135
Ecl136II	GAGCTC	1 cut(s)	919
Eco105I	TACGTA	1 cut(s)	790
Eco130I	CCWWGG	2 cut(s)	27, 417
Eco24I	GRGCYC	3 cut(s)	343, 519, 921
Eco47I	GGWCC	1 cut(s)	857
Eco53kI	GAGCTC	1 cut(s)	919
Eco57I	CTGAAG	2 cut(s)	584, 972
Eco88I	CYCGRG	1 cut(s)	941
EcoICRI	GAGCTC	1 cut(s)	919
EcoO109I	RGGNCCY	1 cut(s)	515
EcoRII	CCWGG	4 cut(s)	490, 826, 846, 862
EcoT14I	CCWWGG	2 cut(s)	27, 417
EcoT38I	GRGCYC	3 cut(s)	343, 519, 921
EgeI	GGCGCC	1 cut(s)	36
EheI	GGCGCC	1 cut(s)	36
ErhI	CCWWGG	2 cut(s)	27, 417
Esp3I	CGTCTC	1 cut(s)	118
FaeI	CATG	4 cut(s)	327, 550, 568, 805
FalI	AAGNNNNNCTT	6 cut(s)	65, 97, 189, 221, 410, 442
FatI	CATG	4 cut(s)	323, 546, 564, 801
FblI	GTMKAC	1 cut(s)	392
Fnu4HI	GCNGC	4 cut(s)	33, 108, 236, 714
FokI	GGATG	3 cut(s)	368, 830, 880
FriOI	GRGCYC	3 cut(s)	343, 519, 921
Fsp4HI	GCNGC	4 cut(s)	33, 108, 236, 714
FspBI	CTAG	2 cut(s)	167, 717
GlaI	GCGC	1 cut(s)	36
GluI	GCNGC	4 cut(s)	33, 108, 236, 714
HaeII	RGCGCY	1 cut(s)	38
HaeIII	GGCC	4 cut(s)	66, 192, 517, 614
HapII	CCGG	3 cut(s)	117, 152, 573
HhaI	GCGC	1 cut(s)	37
Hin1I	GRCGYC	1 cut(s)	35
Hin1II	CATG	4 cut(s)	327, 550, 568, 805
Hin6I	GCGC	1 cut(s)	35
HinP1I	GCGC	1 cut(s)	35
HindIII	AAGCTT	2 cut(s)	79, 481
HinfI	GANTC	2 cut(s)	123, 640
HpaII	CCGG	3 cut(s)	117, 152, 573
HphI	GGTGA	2 cut(s)	842, 899
Hpy166II	GTNNAC	4 cut(s)	282, 393, 948, 964
Hpy188I	TCNGA	4 cut(s)	98, 294, 406, 533
Hpy188III	TCNNGA	7 cut(s)	16, 167, 212, 410, 452, 629, 899
Hpy8I	GTNNAC	4 cut(s)	282, 393, 948, 964
HpyAV	CCTTC	6 cut(s)	19, 55, 103, 356, 449, 505
HpyCH4III	ACNGT	1 cut(s)	748
HpyCH4IV	ACGT	2 cut(s)	315, 789
HpyCH4V	TGCA	4 cut(s)	203, 238, 248, 713
HpyF10VI	GCNNNNNNNGC	3 cut(s)	32, 41, 107
HpyF3I	CTNAG	2 cut(s)	530, 642
HpySE526I	ACGT	2 cut(s)	315, 789
Hsp92I	GRCGYC	1 cut(s)	35
Hsp92II	CATG	4 cut(s)	327, 550, 568, 805
HspAI	GCGC	1 cut(s)	35
KasI	GGCGCC	1 cut(s)	34
Kzo9I	GATC	3 cut(s)	289, 406, 905
LmnI	GCTCC	3 cut(s)	98, 346, 829
Lsp1109I	GCAGC	3 cut(s)	119, 222, 725
LweI	GCATC	2 cut(s)	346, 403
MaeI	CTAG	2 cut(s)	167, 717
MaeII	ACGT	2 cut(s)	315, 789
MaeIII	GTNAC	3 cut(s)	648, 785, 887
MalI	GATC	3 cut(s)	291, 408, 907
MboI	GATC	3 cut(s)	289, 406, 905
MboII	GAAGA	4 cut(s)	4, 562, 845, 965
MhlI	GDGCHC	3 cut(s)	343, 519, 921
MluCI	AATT	5 cut(s)	227, 269, 438, 727, 968
Mly113I	GGCGCC	1 cut(s)	35
MlyI	GAGTC	1 cut(s)	634
MmeI	TCCRAC	2 cut(s)	749, 782
MnlI	CCTC	6 cut(s)	12, 56, 157, 330, 604, 870
MseI	TTAA	8 cut(s)	207, 231, 437, 441, 485, 690, 731, 810
MspI	CCGG	3 cut(s)	117, 152, 573
MspR9I	CCNGG	4 cut(s)	492, 828, 848, 864
Mva1269I	GAATGC	2 cut(s)	87, 301
MvaI	CCWGG	4 cut(s)	492, 828, 848, 864
MwoI	GCNNNNNNNGC	3 cut(s)	32, 41, 107
NarI	GGCGCC	1 cut(s)	35
NdeII	GATC	3 cut(s)	289, 406, 905
NheI	GCTAGC	1 cut(s)	716
NlaIII	CATG	4 cut(s)	327, 550, 568, 805
NlaIV	GGNNCC	4 cut(s)	36, 342, 516, 517
NmuCI	GTSAC	2 cut(s)	648, 887
PacI	TTAATTAA	1 cut(s)	441
PctI	GAATGC	2 cut(s)	87, 301
PfeI	GAWTC	1 cut(s)	123
Pfl23II	CGTACG	1 cut(s)	382
PkrI	GCNGC	4 cut(s)	34, 109, 237, 715
PleI	GAGTC	1 cut(s)	634
PluTI	GGCGCC	1 cut(s)	38
PpsI	GAGTC	1 cut(s)	634
Ppu21I	YACGTR	1 cut(s)	790
Psp124BI	GAGCTC	1 cut(s)	921
Psp6I	CCWGG	4 cut(s)	490, 826, 846, 862
PspGI	CCWGG	4 cut(s)	490, 826, 846, 862
PspLI	CGTACG	1 cut(s)	382
PspN4I	GGNNCC	4 cut(s)	36, 342, 516, 517
PspOMI	GGGCCC	1 cut(s)	515
PspPI	GGNCC	4 cut(s)	191, 515, 516, 857
RsaI	GTAC	3 cut(s)	384, 580, 947
RsaNI	GTAC	3 cut(s)	383, 579, 946
SacI	GAGCTC	1 cut(s)	921
SaqAI	TTAA	8 cut(s)	207, 231, 437, 441, 485, 690, 731, 810
SatI	GCNGC	4 cut(s)	33, 108, 236, 714
Sau3AI	GATC	3 cut(s)	289, 406, 905
Sau96I	GGNCC	4 cut(s)	191, 515, 516, 857
SchI	GAGTC	1 cut(s)	634
ScrFI	CCNGG	4 cut(s)	492, 828, 848, 864
SduI	GDGCHC	3 cut(s)	343, 519, 921
SfaNI	GCATC	2 cut(s)	346, 403
SfcI	CTRYAG	1 cut(s)	747
SfoI	GGCGCC	1 cut(s)	36
SinI	GGWCC	1 cut(s)	857
SmlI	CTYRAG	1 cut(s)	897
SmoI	CTYRAG	1 cut(s)	897
SnaBI	TACGTA	1 cut(s)	790
Sse9I	AATT	5 cut(s)	227, 269, 438, 727, 968
SsiI	CCGC	4 cut(s)	32, 42, 120, 359
SspDI	GGCGCC	1 cut(s)	34
SspMI	CTAG	2 cut(s)	167, 717
SstI	GAGCTC	1 cut(s)	921
StyD4I	CCNGG	4 cut(s)	490, 826, 846, 862
StyI	CCWWGG	2 cut(s)	27, 417
TaaI	ACNGT	1 cut(s)	748
TaiI	ACGT	2 cut(s)	318, 792
TaqI	TCGA	2 cut(s)	61, 71
TaqII	GACCGA	1 cut(s)	918
TasI	AATT	5 cut(s)	227, 269, 438, 727, 968
TauI	GCSGC	1 cut(s)	35
TfiI	GAWTC	1 cut(s)	123
Tru1I	TTAA	8 cut(s)	207, 231, 437, 441, 485, 690, 731, 810
Tru9I	TTAA	8 cut(s)	207, 231, 437, 441, 485, 690, 731, 810
TscAI	CASTG	4 cut(s)	657, 751, 928, 955
TseFI	GTSAC	2 cut(s)	648, 887
TseI	GCWGC	3 cut(s)	107, 235, 713
Tsp45I	GTSAC	2 cut(s)	648, 887
TspDTI	ATGAA	4 cut(s)	186, 312, 563, 887
TspRI	CASTG	4 cut(s)	657, 751, 928, 955
VpaK11BI	GGWCC	1 cut(s)	857
XapI	RAATTY	3 cut(s)	227, 727, 968
XbaI	TCTAGA	1 cut(s)	166
XcmI	CCANNNNNNNNNTGG	1 cut(s)	765
XmiI	GTMKAC	1 cut(s)	392
XspI	CTAG	2 cut(s)	167, 717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.