MD00G1130000.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002898
Physical Location & Seq
Reverse (-)
27929214 .. 27933812
4599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1130000.v1.1.491

Sequence Viewer

Length: 1653 bp
ATGTCCTTACTCTCTGCCATTTCATCCAGAATGTGGTGTCATTGCCGAATGGTGTATTTGCCCATGTCATATTTGTATGGGAAAAGGTTTGTTGGACCTATCACACCCACAATCCGGTCATTGAGGAAGGAGCTTTATACAGCCCCCTATCATGAAGTTGACTGGAATAAAGCTCGCAATCTATGTGCAAAGGTTGGTAAGAAACATCTGCGCTTTCAATATACTCTGCTTCCTCCAGCTCCCCTGTGTAAAGGTCCCGTGTCTGAAGATCTATACTATCCACACCCAATGGTACAAGATGTCTTGTGGGGGTCTCTTCACTATGTTTATGAACCTGTTTTCACACGTTGGCCTGCAAAAGAATTGAGGGACAGTGCTTTGAAGACTGTAATGCAGCATATTCATTATGAAGATGAAAATACTCGGTATATATGCATAGGGCCTGTTAACAAGGTGTTAAATATGCTTTGTTGTTGGGCGGAGGATCCAAACTCAGAAGCATTCAAATTGCATCTCCCGAGAATCCCTGATTATTTGTGGATTGCTGAAGATGGCATGAAAATGCAGGGCTACAATGGAAGTCAAGCATGGGATACTTCTTCTTTTGCCATTCAAGCAATTATATCAACTAACCTGGTTGAAGAATATGGACCTACTTTAAGAAAAGCGCATCAGTATATCAAAGATTCCCAGGTCGTGGAAGATTGTCCTGGAGATCTGGATTATTGGTATCGTCACATTTCAAAAGGTGCTTGGCCATTCTCAACAGCAGATCATGGGTGGCCTATCTCTGACTGCACAGCAGCTGAAGGACTGAAGGCTGCCTTATTACTATCGAAACTTCCAACAGAAACAGTTGGTGAATCATTGGATATAAAGCGATTTTATGATGCTGTAAATGTTACCCTTTCCTTACAGAATGACGATGGTGGTTTTGCGACTTATGAACTAACTAGATCGTATCAGTGGTTAGAGCTAATCAACCCTGCCGAAACTTTTGGTGACATCGTCATTGATTATCCATACGTTGAATGCACCTCAGCAGCAATTCAAGCACTTGCATTGTTCAAAAGGTTATGTCCTGCGCATAGGAGTGATGAAATAGAAAACTGCATTGCTCGTGCTGCAAAGTTCATTGAAACGATACAAGCAACAGATGGATCTTGGTATGGTTCCTGGGGAGTATGCTTCACCTATGCTGGGTGGTTTGGAATAAAGGGGTTGGTTGCTGCGGGAAGGACATACGAAGACTGCTCAAGCATCCGTAAAGCATGCGACTTTTTGTTATCCAAAGAGCTTCCTTCAGGTGGATGGGGAGAAAGCTATCTATCATGTCAGAACAAGGTGTATACAAATCTCAAGGACAATAGGCCACATATAGTTCATACTGCATGGGCTATGTTGGCCCTTATTGGTGCTGGGCAGGCTAAGAGAGACCCAACTCCGTTGCATTGGGCAGCTAGAGTACTAATCAATTCTCAAATGAAAAATGGAGATTTCCCTCAGAAGGAGATCACTGGTGTCTTCAACAAGAACTGTATGATCAGCTATTCAGCCTACCGGAACATTTTTTCCAATTTGGGCCCTCGGAGAATATCGTTGCCAAGTATTGGAGGCTCTCTGAATGATGTCTGTTTATTGTTCAACGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

551

Amino Acids

62.23

Weight (kDa)

6.93

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 194 - 525 3.2e-42 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1086
AccB7I CCANNNNNTGG 3 cut(s) 33, 697, 1610
AccI GTMKAC 1 cut(s) 1349
AciI CCGC 2 cut(s) 479, 1232
AclWI GGATC 3 cut(s) 479, 492, 1168
AcoI YGGCCR 1 cut(s) 755
AcuI CTGAAG 5 cut(s) 285, 567, 828, 836, 1287
AfaI GTAC 2 cut(s) 294, 1467
AfiI CCNNNNNNNGG 7 cut(s) 33, 114, 697, 1200, 1307, 1507, 1610
AflIII ACRYGT 1 cut(s) 344
AjnI CCWGG 4 cut(s) 633, 690, 709, 1175
AloI GAACNNNNNNTCC 2 cut(s) 1556, 1588
AluBI AGCT 9 cut(s) 133, 173, 239, 806, 976, 1297, 1323, 1460, 1548
AluI AGCT 9 cut(s) 133, 173, 239, 806, 976, 1297, 1323, 1460, 1548
Alw26I GTCTC 2 cut(s) 318, 1428
AlwI GGATC 3 cut(s) 479, 492, 1168
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 1 cut(s) 517
AoxI GGCC 7 cut(s) 350, 440, 755, 782, 1370, 1404, 1582
ApaI GGGCCC 1 cut(s) 1586
ApeKI GCWGC 7 cut(s) 394, 803, 821, 1043, 1124, 1229, 1457
AspLEI GCGC 3 cut(s) 213, 670, 1087
AspS9I GGNCC 7 cut(s) 95, 254, 440, 650, 1405, 1582, 1583
AsuHPI GGTGA 3 cut(s) 872, 1013, 1183
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 3 cut(s) 95, 254, 650
BaeGI GKGCMC 1 cut(s) 1586
BalI TGGCCA 1 cut(s) 757
BamHI GGATCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 1586
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 3 cut(s) 389, 1254, 1516
BbvCI CCTCAGC 1 cut(s) 1039
BbvI GCAGC 7 cut(s) 406, 808, 815, 1055, 1111, 1216, 1469
BccI CCATC 4 cut(s) 545, 920, 1151, 1305
BciT130I CCWGG 4 cut(s) 635, 692, 711, 1177
BciVI GTATCC 1 cut(s) 586
BclI TGATCA 1 cut(s) 1542
BcoDI GTCTC 2 cut(s) 318, 1428
BfaI CTAG 2 cut(s) 954, 1461
BfuI GTATCC 1 cut(s) 586
BglII AGATCT 2 cut(s) 268, 715
BisI GCNGC 7 cut(s) 395, 804, 822, 1044, 1125, 1230, 1458
BlsI GCNGC 7 cut(s) 396, 805, 823, 1045, 1126, 1231, 1459
BmcAI AGTACT 1 cut(s) 1467
Bme1390I CCNGG 4 cut(s) 635, 692, 711, 1177
Bme18I GGWCC 3 cut(s) 95, 254, 650
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 7 cut(s) 95, 254, 440, 650, 1405, 1582, 1583
BmiI GGNNCC 4 cut(s) 256, 486, 1174, 1584
BmrFI CCNGG 4 cut(s) 635, 692, 711, 1177
BmsI GCATC 4 cut(s) 520, 679, 880, 1269
BpiI GAAGAC 3 cut(s) 389, 1254, 1516
BpmI CTGGAG 2 cut(s) 219, 732
Bpu10I CCTNAGC 1 cut(s) 1039
BpuEI CTTGAG 2 cut(s) 1240, 1343
BsaI GGTCTC 2 cut(s) 318, 1428
BsaJI CCNNGG 3 cut(s) 690, 1176, 1586
BsaWI WCCGGW 2 cut(s) 114, 1560
Bsc4I CCNNNNNNNGG 7 cut(s) 33, 114, 697, 1200, 1307, 1507, 1610
Bse1I ACTGG 2 cut(s) 167, 1522
Bse3DI GCAATG 2 cut(s) 40, 1113
BseBI CCWGG 4 cut(s) 635, 692, 711, 1177
BseDI CCNNGG 3 cut(s) 690, 1176, 1586
BseGI GGATG 3 cut(s) 23, 1260, 1316
BseLI CCNNNNNNNGG 7 cut(s) 33, 114, 697, 1200, 1307, 1507, 1610
BseMI GCAATG 2 cut(s) 40, 1113
BseMII CTCAG 3 cut(s) 507, 1053, 1517
BseNI ACTGG 2 cut(s) 167, 1522
BseSI GKGCMC 1 cut(s) 1586
BseXI GCAGC 7 cut(s) 406, 808, 815, 1055, 1111, 1216, 1469
BseYI CCCAGC 2 cut(s) 1199, 1418
BsgI GTGCAG 1 cut(s) 781
BshFI GGCC 7 cut(s) 352, 442, 757, 784, 1372, 1406, 1584
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 2 cut(s) 115, 1561
BslFI GGGAC 2 cut(s) 240, 383
BslI CCNNNNNNNGG 7 cut(s) 33, 114, 697, 1200, 1307, 1507, 1610
BsmAI GTCTC 2 cut(s) 318, 1428
BsmFI GGGAC 2 cut(s) 240, 383
BsmI GAATGC 2 cut(s) 500, 1037
BsnI GGCC 7 cut(s) 352, 442, 757, 784, 1372, 1406, 1584
Bso31I GGTCTC 2 cut(s) 318, 1428
BsoBI CYCGRG 1 cut(s) 517
Bsp120I GGGCCC 1 cut(s) 1582
Bsp1286I GDGCHC 1 cut(s) 1586
Bsp143I GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
BspACI CCGC 2 cut(s) 479, 1232
BspANI GGCC 7 cut(s) 352, 442, 757, 784, 1372, 1406, 1584
BspCNI CTCAG 3 cut(s) 506, 1052, 1516
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 4 cut(s) 256, 486, 1174, 1584
BspPI GGATC 3 cut(s) 479, 492, 1168
BspTNI GGTCTC 2 cut(s) 318, 1428
BsrDI GCAATG 2 cut(s) 40, 1113
BsrI ACTGG 2 cut(s) 167, 1522
BssECI CCNNGG 3 cut(s) 690, 1176, 1586
BssMI GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
BssNAI GTATAC 1 cut(s) 1350
BssSI CACGAG 1 cut(s) 1119
Bst1107I GTATAC 1 cut(s) 1350
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 4 cut(s) 635, 692, 711, 1177
Bst4CI ACNGT 4 cut(s) 374, 388, 856, 1538
Bst6I CTCTTC 1 cut(s) 321
BstC8I GCNNGC 4 cut(s) 175, 354, 1273, 1425
BstDEI CTNAG 5 cut(s) 493, 1039, 1428, 1503, 1650
BstF5I GGATG 3 cut(s) 23, 1260, 1316
BstHHI GCGC 3 cut(s) 213, 670, 1087
BstKTI GATC 8 cut(s) 271, 487, 718, 775, 959, 1163, 1515, 1545
BstMAI GTCTC 2 cut(s) 318, 1428
BstMBI GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
BstMWI GCNNNNNNNGC 5 cut(s) 614, 1052, 1124, 1403, 1424
BstNI CCWGG 4 cut(s) 635, 692, 711, 1177
BstNSI RCATGY 1 cut(s) 1275
BstSCI CCNGG 4 cut(s) 633, 690, 709, 1175
BstSLI GKGCMC 1 cut(s) 1586
BstV1I GCAGC 7 cut(s) 406, 808, 815, 1055, 1111, 1216, 1469
BstV2I GAAGAC 3 cut(s) 389, 1254, 1516
BstX2I RGATCY 4 cut(s) 268, 484, 715, 1160
BstYI RGATCY 4 cut(s) 268, 484, 715, 1160
BstZ17I GTATAC 1 cut(s) 1350
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 7 cut(s) 352, 442, 757, 784, 1372, 1406, 1584
BtsCI GGATG 3 cut(s) 23, 1260, 1316
BtsIMutI CAGTG 3 cut(s) 379, 971, 1515
Cac8I GCNNGC 4 cut(s) 175, 354, 1273, 1425
CaiI CAGNNNCTG 1 cut(s) 806
CciI TCATGA 1 cut(s) 151
CfoI GCGC 3 cut(s) 213, 670, 1087
Cfr13I GGNCC 7 cut(s) 95, 254, 440, 650, 1405, 1582, 1583
CsiI ACCWGGT 1 cut(s) 633
Csp6I GTAC 2 cut(s) 293, 1466
CviAII CATG 8 cut(s) 64, 152, 556, 588, 776, 1272, 1332, 1392
CviQI GTAC 2 cut(s) 293, 1466
DdeI CTNAG 5 cut(s) 493, 1039, 1428, 1503, 1650
DpnI GATC 8 cut(s) 270, 486, 717, 774, 958, 1162, 1514, 1544
DpnII GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
EaeI YGGCCR 1 cut(s) 755
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
EciI GGCGGA 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 1586
Eco31I GGTCTC 2 cut(s) 318, 1428
Eco47I GGWCC 3 cut(s) 95, 254, 650
Eco57I CTGAAG 5 cut(s) 285, 567, 828, 836, 1287
Eco88I CYCGRG 1 cut(s) 517
EcoO109I RGGNCCY 3 cut(s) 254, 440, 1583
EcoRII CCWGG 4 cut(s) 633, 690, 709, 1175
EcoT22I ATGCAT 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 1586
FaeI CATG 8 cut(s) 67, 155, 559, 591, 779, 1275, 1335, 1395
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 2 cut(s) 240, 383
FatI CATG 8 cut(s) 63, 151, 555, 587, 775, 1271, 1331, 1391
FauI CCCGC 1 cut(s) 1225
FbaI TGATCA 1 cut(s) 1542
FblI GTMKAC 1 cut(s) 1349
Fnu4HI GCNGC 7 cut(s) 395, 804, 822, 1044, 1125, 1230, 1458
FokI GGATG 3 cut(s) 10, 1247, 1323
FriOI GRGCYC 1 cut(s) 1586
Fsp4HI GCNGC 7 cut(s) 395, 804, 822, 1044, 1125, 1230, 1458
FspBI CTAG 2 cut(s) 954, 1461
FspI TGCGCA 1 cut(s) 1086
GlaI GCGC 3 cut(s) 212, 669, 1086
GluI GCNGC 7 cut(s) 395, 804, 822, 1044, 1125, 1230, 1458
GsaI CCCAGC 2 cut(s) 1203, 1422
GsuI CTGGAG 2 cut(s) 219, 732
HaeIII GGCC 7 cut(s) 352, 442, 757, 784, 1372, 1406, 1584
HapII CCGG 2 cut(s) 115, 1561
HhaI GCGC 3 cut(s) 213, 670, 1087
Hin1II CATG 8 cut(s) 67, 155, 559, 591, 779, 1275, 1335, 1395
Hin6I GCGC 3 cut(s) 211, 668, 1085
HinP1I GCGC 3 cut(s) 211, 668, 1085
HincII GTYRAC 2 cut(s) 160, 448
HindII GTYRAC 2 cut(s) 160, 448
HinfI GANTC 3 cut(s) 522, 686, 863
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 2 cut(s) 115, 1561
HphI GGTGA 3 cut(s) 872, 1013, 1183
Hpy166II GTNNAC 3 cut(s) 160, 448, 1350
Hpy188I TCNGA 7 cut(s) 265, 496, 793, 1338, 1506, 1590, 1623
Hpy188III TCNNGA 4 cut(s) 27, 152, 517, 719
Hpy8I GTNNAC 3 cut(s) 160, 448, 1350
HpyAV CCTTC 6 cut(s) 121, 803, 811, 1230, 1311, 1501
HpyCH4III ACNGT 4 cut(s) 374, 388, 856, 1538
HpyCH4IV ACGT 2 cut(s) 346, 1026
HpyF10VI GCNNNNNNNGC 5 cut(s) 614, 1052, 1124, 1403, 1424
HpyF3I CTNAG 5 cut(s) 493, 1039, 1428, 1503, 1650
HpySE526I ACGT 2 cut(s) 346, 1026
Hsp92II CATG 8 cut(s) 67, 155, 559, 591, 779, 1275, 1335, 1395
HspAI GCGC 3 cut(s) 211, 668, 1085
Ksp22I TGATCA 1 cut(s) 1542
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
LmnI GCTCC 2 cut(s) 130, 244
Lsp1109I GCAGC 7 cut(s) 406, 808, 815, 1055, 1111, 1216, 1469
LweI GCATC 4 cut(s) 520, 679, 880, 1269
MabI ACCWGGT 1 cut(s) 633
MaeI CTAG 2 cut(s) 954, 1461
MaeII ACGT 2 cut(s) 346, 1026
MaeIII GTNAC 3 cut(s) 734, 901, 1001
MalI GATC 8 cut(s) 270, 486, 717, 774, 958, 1162, 1514, 1544
MboI GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
MflI RGATCY 4 cut(s) 268, 484, 715, 1160
MhlI GDGCHC 1 cut(s) 1586
MlsI TGGCCA 1 cut(s) 757
MluCI AATT 6 cut(s) 362, 506, 618, 1047, 1474, 1576
MluNI TGGCCA 1 cut(s) 757
MmeI TCCRAC 2 cut(s) 73, 869
MnlI CCTC 8 cut(s) 117, 243, 360, 475, 1048, 1512, 1596, 1607
Mox20I TGGCCA 1 cut(s) 757
Mph1103I ATGCAT 1 cut(s) 437
MscI TGGCCA 1 cut(s) 757
MseI TTAA 3 cut(s) 447, 458, 659
MslI CAYNNNNRTG 2 cut(s) 560, 1092
Msp20I TGGCCA 1 cut(s) 757
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 2 cut(s) 115, 1561
MspR9I CCNGG 4 cut(s) 635, 692, 711, 1177
Mva1269I GAATGC 2 cut(s) 500, 1037
MvaI CCWGG 4 cut(s) 635, 692, 711, 1177
MwoI GCNNNNNNNGC 5 cut(s) 614, 1052, 1124, 1403, 1424
NdeII GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
NlaIII CATG 8 cut(s) 67, 155, 559, 591, 779, 1275, 1335, 1395
NlaIV GGNNCC 4 cut(s) 256, 486, 1174, 1584
NmuCI GTSAC 2 cut(s) 734, 1001
NsbI TGCGCA 1 cut(s) 1086
NsiI ATGCAT 1 cut(s) 437
NspI RCATGY 1 cut(s) 1275
PaeI GCATGC 1 cut(s) 1275
PagI TCATGA 1 cut(s) 151
PctI GAATGC 2 cut(s) 500, 1037
PfeI GAWTC 3 cut(s) 522, 686, 863
PflFI GACNNNGTC 1 cut(s) 1007
PflMI CCANNNNNTGG 3 cut(s) 33, 697, 1610
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 7 cut(s) 396, 805, 823, 1045, 1126, 1231, 1459
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 4 cut(s) 633, 690, 709, 1175
PspFI CCCAGC 2 cut(s) 1199, 1418
PspGI CCWGG 4 cut(s) 633, 690, 709, 1175
PspN4I GGNNCC 4 cut(s) 256, 486, 1174, 1584
PspOMI GGGCCC 1 cut(s) 1582
PspPI GGNCC 7 cut(s) 95, 254, 440, 650, 1405, 1582, 1583
PspPPI RGGWCCY 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 4 cut(s) 268, 484, 715, 1160
PsyI GACNNNGTC 1 cut(s) 1007
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 2 cut(s) 294, 1467
RsaNI GTAC 2 cut(s) 293, 1466
RseI CAYNNNNRTG 2 cut(s) 560, 1092
SaqAI TTAA 3 cut(s) 447, 458, 659
SatI GCNGC 7 cut(s) 395, 804, 822, 1044, 1125, 1230, 1458
Sau3AI GATC 8 cut(s) 268, 484, 715, 772, 956, 1160, 1512, 1542
Sau96I GGNCC 7 cut(s) 95, 254, 440, 650, 1405, 1582, 1583
ScaI AGTACT 1 cut(s) 1467
ScrFI CCNGG 4 cut(s) 635, 692, 711, 1177
SduI GDGCHC 1 cut(s) 1586
SexAI ACCWGGT 1 cut(s) 633
SfaNI GCATC 4 cut(s) 520, 679, 880, 1269
SinI GGWCC 3 cut(s) 95, 254, 650
SmiMI CAYNNNNRTG 2 cut(s) 560, 1092
SmlI CTYRAG 2 cut(s) 1255, 1358
SmoI CTYRAG 2 cut(s) 1255, 1358
SphI GCATGC 1 cut(s) 1275
Sse9I AATT 6 cut(s) 362, 506, 618, 1047, 1474, 1576
SsiI CCGC 2 cut(s) 479, 1232
SspMI CTAG 2 cut(s) 954, 1461
StyD4I CCNGG 4 cut(s) 633, 690, 709, 1175
TaaI ACNGT 4 cut(s) 374, 388, 856, 1538
TaiI ACGT 2 cut(s) 349, 1029
TaqI TCGA 1 cut(s) 836
TasI AATT 6 cut(s) 362, 506, 618, 1047, 1474, 1576
TatI WGTACW 1 cut(s) 1465
TfiI GAWTC 3 cut(s) 522, 686, 863
Tru1I TTAA 3 cut(s) 447, 458, 659
Tru9I TTAA 3 cut(s) 447, 458, 659
TscAI CASTG 3 cut(s) 379, 971, 1522
TseFI GTSAC 2 cut(s) 734, 1001
TseI GCWGC 7 cut(s) 394, 803, 821, 1043, 1124, 1229, 1457
Tsp45I GTSAC 2 cut(s) 734, 1001
TspGWI ACGGA 2 cut(s) 1253, 1434
TspRI CASTG 3 cut(s) 379, 971, 1522
Tth111I GACNNNGTC 1 cut(s) 1007
Van91I CCANNNNNTGG 3 cut(s) 33, 697, 1610
VpaK11BI GGWCC 3 cut(s) 95, 254, 650
XceI RCATGY 1 cut(s) 1275
XmiI GTMKAC 1 cut(s) 1349
XspI CTAG 2 cut(s) 954, 1461
ZrmI AGTACT 1 cut(s) 1467
Zsp2I ATGCAT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.