Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002898

Physical Location & Seq

Genomic Coordinates Reverse (-)

52304098 .. 52310388

Gene Structure 6291 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1220900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGAGAATTCAGTTTGCAAAGGAGAATCCACGTGCCACTAATCTACCGCAACTCAAAGTTAAAGATCAAGAAGAGGTGAAGGAAGAGGCAGTGGCAACCACTTTACGAAGGGCTCTCAGTTTTTATTCAACAATCCAGGCGCATGACGGTCATTGGGCTGGGGATTATGGGGGTCCCATGTTTCTGCTCCCGGGTTTGATCATTACACTTTCAATTACTGGAGCTCTAAATGCTGTGTTATCGAAAGAGCATCAACGTGAGATGTGCAGATATCTTTATAATCACCAGAATGAAGATGGGGGATGGGGTCTGCATATCGAGGGGCCAAGTACCATGTTTGGTACTGCACTAAATTATGTTACTTTGAGGTTGCTTGGCGAAGGTGCAGATGATGGACAAGGGGCAATCGAACTTGCACGGAAATGGATATTAGACCATGGTGGCGTGACTGCAATTACTTCTTGGGGCAAAATGTGGCTTTCGGTACTTGGGGCATATGAATGGTCCGGGAATAATCCACTTCCGCCTGAAGTATGGCTATGTCCTTACTCTCTGCCATTTCATCCAGGAAGAATGTGGTGTCATTGCCGAATGGTGTATTTGCCCATGTCATATTTGTATGGGAAAAGGTTTGTTGGACCTATCACACCCACAATCCGGTCATTGAGGAAGGAGCTTTATACAGCCCCCTATCATGAAGTTGACTGGAATAAAGCTCGCAATCTATGTGCAAAGATCTATACTATCCACACCCAATGGTACAAGATGTCTTGTGGGGGTCTCTTTCACTATGTTTATGAACCTGTTTTCACACGTTGGCCTGCAAAAGAATTGAGGGACAGTGCTTTGAAGACTGTAATGCAGCATATTCATTATGAAGATGAAAATACTCGGTATATATGCATAGGGCCTGTTAACAAGGTGTTAAATATGCTTTGTTGTTGGGCGGAGGATCCAAACTCAGAAGCATTCAAATTGCATCTCCCGAGAATCCCTGATTATTTGTGGATTGCTGAAGATGGCATGAAAATGCAGGGCTACAATGGAAGTCAAGCATGGGATACTTCTTTTGCCATTCAAGCAATTATATCAACTAACCTGGTTGAAGAATATGGACCTACTTTAAGAAAAGCGCATCAGTATATCAAAGATTCCCAGGTCGTGGAAGATTGTCCTGGAGATCTGGATTATTGGTATCGTCACATTTCAAAAGGTGCTTGGCCATTCTCAACAGCAGATCATGGGTGGCCTATCTCTGACTGCACAGCTGAAGGACTGAAGGCTGCCTTATTACTATCGAAACTTCCAACAGAAACAGTTGGTGAATCATTGGATATAAAGCGATTTTATGATGCTGTAAATGTTACCCTTTCCTTACAGAATGACGATGGTGGTTTTGCGACTTATGAACTAACTAGATCGTATCAGTGGTTAGAGCTAATCAACCCTGCCGAAACTTTTGGTGACATCGTCATTGATTATCCATACGTTGAATGCACCTCAGCAGCAATTCAAGCACTTGCATTGTTCAAAAGGTTATGTCCTGCGCATAGGAGTGATGAAATAGAAAACTGCATTGCTCGTGCTGCAAAGTTCATTGAAACGATACAAGCAACAGATGGATCTTGGTATGGTTCCTGGGGAGTATGCTTCACCTATGCTGGGTGGTTTGGAATAAAGGGGTTGGTTGCTGCGGGAAGGACATACGAAGACTGCTCAAGCATCCGTAAAGCATGCGACTTTTTGTTATCCAAAGAGCTTCCTTCAGGTGGATGGGGAGAAAGCTATCTATCATGTCAGAACAAGGACAATAGGCCACATATAGTTCATACTGCATGGGCTATGTTGGCCCTTATTGGTGCTGGGCAGGCTAAGAGAGACCCAACTCCGTTGCATTGGGCAGCTAGAGTACTAATCAATTCTCAAATGAAAAATGGAGATTTCCCTCAGAAGGAGATCACTGGTGTCTTCAACAAGAACTGTATGATCAGCTATTCAGCCTACCGGAACATTTTTCCAATTTGGGCCCTCGGAGAATATCGTTGCCAAGTATTGGAGGCTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 map00100 map01100 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

689

Amino Acids

77.95

Weight (kDa)

6.18

Isoelectric Point (pI)

39.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	37 - 336	3.7e-38	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	350 - 681	2.3e-47	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07050 AT2G07050
fragaria_vesca	FvH4_4g05990 FvH4_4g05990 FvH4_4g05990 FvH4_4g05990 FvH4_6g36510 FvH4_6g36510
malus_domestica	MD00G1130000.v1.1 MD00G1220900.v1.1 MD13G1232100.v1.1 MD16G1237000.v1.1 MD17G1159900.v1.1 MD17G1245900.v1.1 MD17G1246400.v1.1
prunus_persica	Prupe.1G063400_v2.0.a1 Prupe.3G026100_v2.0.a1 Prupe.3G026100_v2.0.a1
pyrus_communis	pycom09g08550
rosa_chinensis	RchiOBHm_Chr2g0148711 RchiOBHm_Chr2g0148821 RchiOBHm_Chr2g0148951 RchiOBHm_Chr4g0397221
rosa_laevigata	RLG00000009438 RLG00000020359
rosa_multiflora	Rmu_sc0001796.1_g000004 Rmu_sc0002312.1_g000065 Rmu_sc0008532.1_g000008 Rmu_sc0017689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098550 Rroxscaffold_5G00342160
rosa_rugosa	Rorug03G0225900 Rorug03G0343900 Rorug03G0343900
rosa_samantha	Rh2AG467200 Rh2DG488900
rosa_wichuraiana	Rw2G038090 Rw4G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	279
Acc16I	TGCGCA	1 cut(s)	1548
AccB7I	CCANNNNNTGG	2 cut(s)	1162, 2053
AciI	CCGC	4 cut(s)	47, 524, 947, 1694
AclWI	GGATC	3 cut(s)	947, 960, 1630
AcoI	YGGCCR	1 cut(s)	1220
AcsI	RAATTY	1 cut(s)	6
AcuI	CTGAAG	5 cut(s)	549, 1035, 1290, 1298, 1749
AcvI	CACGTG	1 cut(s)	32
AfaI	GTAC	5 cut(s)	331, 343, 486, 761, 1911
AfiI	CCNNNNNNNGG	6 cut(s)	657, 1162, 1662, 1769, 1951, 2053
AflIII	ACRYGT	1 cut(s)	812
AjnI	CCWGG	6 cut(s)	135, 565, 1098, 1155, 1174, 1637
AluBI	AGCT	9 cut(s)	224, 676, 716, 1268, 1438, 1759, 1785, 1904, 1992
AluI	AGCT	9 cut(s)	224, 676, 716, 1268, 1438, 1759, 1785, 1904, 1992
Alw21I	GWGCWC	1 cut(s)	226
Alw26I	GTCTC	2 cut(s)	785, 1872
AlwI	GGATC	3 cut(s)	947, 960, 1630
Ama87I	CYCGRG	2 cut(s)	190, 985
AoxI	GGCC	8 cut(s)	323, 818, 908, 1220, 1247, 1814, 1848, 2025
ApaI	GGGCCC	1 cut(s)	2029
ApeKI	GCWGC	6 cut(s)	862, 1283, 1505, 1586, 1691, 1901
ApoI	RAATTY	1 cut(s)	6
AspLEI	GCGC	3 cut(s)	142, 1135, 1549
AspS9I	GGNCC	9 cut(s)	173, 323, 504, 638, 908, 1115, 1849, 2025, 2026
AsuC2I	CCSGG	3 cut(s)	191, 192, 508
AsuHPI	GGTGA	5 cut(s)	88, 275, 1334, 1475, 1645
AvaI	CYCGRG	2 cut(s)	190, 985
AvaII	GGWCC	4 cut(s)	173, 504, 638, 1115
BaeGI	GKGCMC	1 cut(s)	2029
BalI	TGGCCA	1 cut(s)	1222
BamHI	GGATCC	1 cut(s)	952
BanII	GRGCYC	3 cut(s)	115, 226, 2029
BauI	CACGAG	1 cut(s)	1581
BbrPI	CACGTG	1 cut(s)	32
BbsI	GAAGAC	3 cut(s)	857, 1716, 1960
Bbv12I	GWGCWC	1 cut(s)	226
BbvCI	CCTCAGC	1 cut(s)	1501
BbvI	GCAGC	6 cut(s)	874, 1270, 1517, 1573, 1678, 1913
BccI	CCATC	7 cut(s)	290, 297, 386, 1013, 1382, 1613, 1767
BciT130I	CCWGG	6 cut(s)	137, 567, 1100, 1157, 1176, 1639
BciVI	GTATCC	1 cut(s)	1054
BclI	TGATCA	2 cut(s)	198, 1986
BcnI	CCSGG	3 cut(s)	191, 192, 508
BcoDI	GTCTC	2 cut(s)	785, 1872
BfaI	CTAG	2 cut(s)	1416, 1905
BfuI	GTATCC	1 cut(s)	1054
BglII	AGATCT	2 cut(s)	735, 1180
BisI	GCNGC	6 cut(s)	863, 1284, 1506, 1587, 1692, 1902
BlsI	GCNGC	6 cut(s)	864, 1285, 1507, 1588, 1693, 1903
BmcAI	AGTACT	1 cut(s)	1911
Bme1390I	CCNGG	9 cut(s)	137, 191, 192, 508, 567, 1100, 1157, 1176, 1639
Bme18I	GGWCC	4 cut(s)	173, 504, 638, 1115
BmeT110I	CYCGRG	2 cut(s)	190, 985
BmgT120I	GGNCC	9 cut(s)	173, 323, 504, 638, 908, 1115, 1849, 2025, 2026
BmiI	GGNNCC	6 cut(s)	174, 175, 324, 954, 1636, 2027
BmrFI	CCNGG	9 cut(s)	137, 191, 192, 508, 567, 1100, 1157, 1176, 1639
BmsI	GCATC	5 cut(s)	259, 988, 1144, 1342, 1731
BpiI	GAAGAC	3 cut(s)	857, 1716, 1960
BpmI	CTGGAG	2 cut(s)	240, 1197
Bpu10I	CCTNAGC	1 cut(s)	1501
BpuEI	CTTGAG	1 cut(s)	1702
BpuMI	CCSGG	3 cut(s)	191, 192, 508
BsaAI	YACGTR	1 cut(s)	32
BsaI	GGTCTC	2 cut(s)	785, 1872
BsaJI	CCNNGG	5 cut(s)	190, 436, 1155, 1638, 2029
BsaWI	WCCGGW	2 cut(s)	657, 2004
Bsc4I	CCNNNNNNNGG	6 cut(s)	657, 1162, 1662, 1769, 1951, 2053
Bse1I	ACTGG	3 cut(s)	223, 710, 1966
Bse3DI	GCAATG	2 cut(s)	583, 1575
BseBI	CCWGG	6 cut(s)	137, 567, 1100, 1157, 1176, 1639
BseDI	CCNNGG	5 cut(s)	190, 436, 1155, 1638, 2029
BseGI	GGATG	4 cut(s)	308, 562, 1722, 1778
BseLI	CCNNNNNNNGG	6 cut(s)	657, 1162, 1662, 1769, 1951, 2053
BseMI	GCAATG	2 cut(s)	583, 1575
BseMII	CTCAG	4 cut(s)	130, 975, 1515, 1961
BseNI	ACTGG	3 cut(s)	223, 710, 1966
BseSI	GKGCMC	1 cut(s)	2029
BseXI	GCAGC	6 cut(s)	874, 1270, 1517, 1573, 1678, 1913
BseYI	CCCAGC	3 cut(s)	158, 1661, 1862
BsgI	GTGCAG	4 cut(s)	286, 330, 405, 1246
BshFI	GGCC	8 cut(s)	325, 820, 910, 1222, 1249, 1816, 1850, 2027
BsiHKAI	GWGCWC	1 cut(s)	226
BsiHKCI	CYCGRG	2 cut(s)	190, 985
BsiSI	CCGG	4 cut(s)	191, 507, 658, 2005
BslFI	GGGAC	2 cut(s)	159, 851
BslI	CCNNNNNNNGG	6 cut(s)	657, 1162, 1662, 1769, 1951, 2053
BsmAI	GTCTC	2 cut(s)	785, 1872
BsmFI	GGGAC	2 cut(s)	159, 851
BsmI	GAATGC	2 cut(s)	968, 1499
BsnI	GGCC	8 cut(s)	325, 820, 910, 1222, 1249, 1816, 1850, 2027
Bso31I	GGTCTC	2 cut(s)	785, 1872
BsoBI	CYCGRG	2 cut(s)	190, 985
Bsp120I	GGGCCC	1 cut(s)	2025
Bsp1286I	GDGCHC	3 cut(s)	115, 226, 2029
Bsp19I	CCATGG	1 cut(s)	436
BspACI	CCGC	4 cut(s)	47, 524, 947, 1694
BspANI	GGCC	8 cut(s)	325, 820, 910, 1222, 1249, 1816, 1850, 2027
BspCNI	CTCAG	4 cut(s)	129, 974, 1514, 1960
BspHI	TCATGA	1 cut(s)	694
BspLI	GGNNCC	6 cut(s)	174, 175, 324, 954, 1636, 2027
BspPI	GGATC	3 cut(s)	947, 960, 1630
BspTNI	GGTCTC	2 cut(s)	785, 1872
BsrDI	GCAATG	2 cut(s)	583, 1575
BsrI	ACTGG	3 cut(s)	223, 710, 1966
BssECI	CCNNGG	5 cut(s)	190, 436, 1155, 1638, 2029
BssSI	CACGAG	1 cut(s)	1581
BssT1I	CCWWGG	1 cut(s)	436
Bst2BI	CACGAG	1 cut(s)	1581
Bst2UI	CCWGG	6 cut(s)	137, 567, 1100, 1157, 1176, 1639
Bst4CI	ACNGT	5 cut(s)	149, 842, 856, 1318, 1982
Bst6I	CTCTTC	2 cut(s)	66, 78
BstBAI	YACGTR	1 cut(s)	32
BstC8I	GCNNGC	4 cut(s)	718, 822, 1735, 1869
BstDEI	CTNAG	5 cut(s)	116, 961, 1501, 1872, 1947
BstDSI	CCRYGG	1 cut(s)	436
BstF5I	GGATG	4 cut(s)	308, 562, 1722, 1778
BstHHI	GCGC	3 cut(s)	142, 1135, 1549
BstMAI	GTCTC	2 cut(s)	785, 1872
BstMWI	GCNNNNNNNGC	6 cut(s)	230, 1079, 1514, 1586, 1847, 1868
BstNI	CCWGG	6 cut(s)	137, 567, 1100, 1157, 1176, 1639
BstNSI	RCATGY	1 cut(s)	1737
BstSCI	CCNGG	9 cut(s)	135, 189, 190, 506, 565, 1098, 1155, 1174, 1637
BstSLI	GKGCMC	1 cut(s)	2029
BstV1I	GCAGC	6 cut(s)	874, 1270, 1517, 1573, 1678, 1913
BstV2I	GAAGAC	3 cut(s)	857, 1716, 1960
BstX2I	RGATCY	4 cut(s)	735, 952, 1180, 1622
BstYI	RGATCY	4 cut(s)	735, 952, 1180, 1622
BsuI	GTATCC	1 cut(s)	1054
BsuRI	GGCC	8 cut(s)	325, 820, 910, 1222, 1249, 1816, 1850, 2027
BtgI	CCRYGG	1 cut(s)	436
BtsCI	GGATG	4 cut(s)	308, 562, 1722, 1778
BtsI	GCAGTG	1 cut(s)	96
BtsIMutI	CAGTG	4 cut(s)	96, 847, 1433, 1959
Cac8I	GCNNGC	4 cut(s)	718, 822, 1735, 1869
CciI	TCATGA	1 cut(s)	694
CfoI	GCGC	3 cut(s)	142, 1135, 1549
Cfr13I	GGNCC	9 cut(s)	173, 323, 504, 638, 908, 1115, 1849, 2025, 2026
Cfr9I	CCCGGG	1 cut(s)	190
CsiI	ACCWGGT	1 cut(s)	1098
Csp6I	GTAC	5 cut(s)	330, 342, 485, 760, 1910
CviQI	GTAC	5 cut(s)	330, 342, 485, 760, 1910
DdeI	CTNAG	5 cut(s)	116, 961, 1501, 1872, 1947
EaeI	YGGCCR	1 cut(s)	1220
Eam1104I	CTCTTC	2 cut(s)	66, 78
EarI	CTCTTC	2 cut(s)	66, 78
EciI	GGCGGA	2 cut(s)	513, 962
Ecl136II	GAGCTC	1 cut(s)	224
Eco130I	CCWWGG	1 cut(s)	436
Eco24I	GRGCYC	3 cut(s)	115, 226, 2029
Eco31I	GGTCTC	2 cut(s)	785, 1872
Eco32I	GATATC	1 cut(s)	272
Eco47I	GGWCC	4 cut(s)	173, 504, 638, 1115
Eco53kI	GAGCTC	1 cut(s)	224
Eco57I	CTGAAG	5 cut(s)	549, 1035, 1290, 1298, 1749
Eco72I	CACGTG	1 cut(s)	32
Eco88I	CYCGRG	2 cut(s)	190, 985
EcoICRI	GAGCTC	1 cut(s)	224
EcoO109I	RGGNCCY	3 cut(s)	173, 908, 2026
EcoRI	GAATTC	1 cut(s)	6
EcoRII	CCWGG	6 cut(s)	135, 565, 1098, 1155, 1174, 1637
EcoRV	GATATC	1 cut(s)	272
EcoT14I	CCWWGG	1 cut(s)	436
EcoT22I	ATGCAT	1 cut(s)	905
EcoT38I	GRGCYC	3 cut(s)	115, 226, 2029
ErhI	CCWWGG	1 cut(s)	436
FalI	AAGNNNNNCTT	2 cut(s)	1271, 1303
FaqI	GGGAC	2 cut(s)	159, 851
FauI	CCCGC	1 cut(s)	1687
FauNDI	CATATG	1 cut(s)	496
FbaI	TGATCA	2 cut(s)	198, 1986
Fnu4HI	GCNGC	6 cut(s)	863, 1284, 1506, 1587, 1692, 1902
FokI	GGATG	4 cut(s)	315, 549, 1709, 1785
FriOI	GRGCYC	3 cut(s)	115, 226, 2029
Fsp4HI	GCNGC	6 cut(s)	863, 1284, 1506, 1587, 1692, 1902
FspBI	CTAG	2 cut(s)	1416, 1905
FspI	TGCGCA	1 cut(s)	1548
GlaI	GCGC	3 cut(s)	141, 1134, 1548
GluI	GCNGC	6 cut(s)	863, 1284, 1506, 1587, 1692, 1902
GsaI	CCCAGC	3 cut(s)	162, 1665, 1866
GsuI	CTGGAG	2 cut(s)	240, 1197
HaeIII	GGCC	8 cut(s)	325, 820, 910, 1222, 1249, 1816, 1850, 2027
HapII	CCGG	4 cut(s)	191, 507, 658, 2005
HhaI	GCGC	3 cut(s)	142, 1135, 1549
Hin6I	GCGC	3 cut(s)	140, 1133, 1547
HinP1I	GCGC	3 cut(s)	140, 1133, 1547
HincII	GTYRAC	2 cut(s)	703, 916
HindII	GTYRAC	2 cut(s)	703, 916
HinfI	GANTC	4 cut(s)	25, 990, 1151, 1325
HpaI	GTTAAC	1 cut(s)	916
HpaII	CCGG	4 cut(s)	191, 507, 658, 2005
HphI	GGTGA	5 cut(s)	88, 275, 1334, 1475, 1645
Hpy166II	GTNNAC	2 cut(s)	703, 916
Hpy188I	TCNGA	6 cut(s)	964, 1258, 1800, 1950, 2033, 2066
Hpy188III	TCNNGA	4 cut(s)	68, 695, 985, 1184
Hpy8I	GTNNAC	2 cut(s)	703, 916
HpyAV	CCTTC	9 cut(s)	73, 102, 374, 664, 1265, 1273, 1692, 1773, 1945
HpyCH4III	ACNGT	5 cut(s)	149, 842, 856, 1318, 1982
HpyCH4IV	ACGT	4 cut(s)	31, 256, 814, 1488
HpyF10VI	GCNNNNNNNGC	6 cut(s)	230, 1079, 1514, 1586, 1847, 1868
HpyF3I	CTNAG	5 cut(s)	116, 961, 1501, 1872, 1947
HpySE526I	ACGT	4 cut(s)	31, 256, 814, 1488
HspAI	GCGC	3 cut(s)	140, 1133, 1547
KflI	GGGWCCC	1 cut(s)	173
Ksp22I	TGATCA	2 cut(s)	198, 1986
KspAI	GTTAAC	1 cut(s)	916
LmnI	GCTCC	3 cut(s)	192, 221, 673
Lsp1109I	GCAGC	6 cut(s)	874, 1270, 1517, 1573, 1678, 1913
LweI	GCATC	5 cut(s)	259, 988, 1144, 1342, 1731
MabI	ACCWGGT	1 cut(s)	1098
MaeI	CTAG	2 cut(s)	1416, 1905
MaeII	ACGT	4 cut(s)	31, 256, 814, 1488
MaeIII	GTNAC	5 cut(s)	358, 445, 1199, 1363, 1463
MflI	RGATCY	4 cut(s)	735, 952, 1180, 1622
MhlI	GDGCHC	3 cut(s)	115, 226, 2029
MlsI	TGGCCA	1 cut(s)	1222
MluNI	TGGCCA	1 cut(s)	1222
MmeI	TCCRAC	2 cut(s)	616, 1331
Mox20I	TGGCCA	1 cut(s)	1222
Mph1103I	ATGCAT	1 cut(s)	905
MscI	TGGCCA	1 cut(s)	1222
MseI	TTAA	4 cut(s)	60, 915, 926, 1124
MslI	CAYNNNNRTG	6 cut(s)	255, 288, 421, 499, 1028, 1554
Msp20I	TGGCCA	1 cut(s)	1222
MspA1I	CMGCKG	1 cut(s)	1268
MspI	CCGG	4 cut(s)	191, 507, 658, 2005
MspR9I	CCNGG	9 cut(s)	137, 191, 192, 508, 567, 1100, 1157, 1176, 1639
Mva1269I	GAATGC	2 cut(s)	968, 1499
MvaI	CCWGG	6 cut(s)	137, 567, 1100, 1157, 1176, 1639
MwoI	GCNNNNNNNGC	6 cut(s)	230, 1079, 1514, 1586, 1847, 1868
NciI	CCSGG	3 cut(s)	191, 192, 508
NcoI	CCATGG	1 cut(s)	436
NdeI	CATATG	1 cut(s)	496
NlaIV	GGNNCC	6 cut(s)	174, 175, 324, 954, 1636, 2027
NmuCI	GTSAC	3 cut(s)	445, 1199, 1463
NsbI	TGCGCA	1 cut(s)	1548
NsiI	ATGCAT	1 cut(s)	905
NspI	RCATGY	1 cut(s)	1737
PaeI	GCATGC	1 cut(s)	1737
PagI	TCATGA	1 cut(s)	694
PctI	GAATGC	2 cut(s)	968, 1499
PfeI	GAWTC	4 cut(s)	25, 990, 1151, 1325
PflFI	GACNNNGTC	1 cut(s)	1469
PflMI	CCANNNNNTGG	2 cut(s)	1162, 2053
PfoI	TCCNGGA	3 cut(s)	506, 565, 1174
PkrI	GCNGC	6 cut(s)	864, 1285, 1507, 1588, 1693, 1903
PmaCI	CACGTG	1 cut(s)	32
PmlI	CACGTG	1 cut(s)	32
Ppu21I	YACGTR	1 cut(s)	32
PpuMI	RGGWCCY	1 cut(s)	173
PsiI	TTATAA	1 cut(s)	279
Psp124BI	GAGCTC	1 cut(s)	226
Psp5II	RGGWCCY	1 cut(s)	173
Psp6I	CCWGG	6 cut(s)	135, 565, 1098, 1155, 1174, 1637
PspCI	CACGTG	1 cut(s)	32
PspFI	CCCAGC	3 cut(s)	158, 1661, 1862
PspGI	CCWGG	6 cut(s)	135, 565, 1098, 1155, 1174, 1637
PspN4I	GGNNCC	6 cut(s)	174, 175, 324, 954, 1636, 2027
PspOMI	GGGCCC	1 cut(s)	2025
PspPI	GGNCC	9 cut(s)	173, 323, 504, 638, 908, 1115, 1849, 2025, 2026
PspPPI	RGGWCCY	1 cut(s)	173
PsuI	RGATCY	4 cut(s)	735, 952, 1180, 1622
PsyI	GACNNNGTC	1 cut(s)	1469
PvuII	CAGCTG	1 cut(s)	1268
RsaI	GTAC	5 cut(s)	331, 343, 486, 761, 1911
RsaNI	GTAC	5 cut(s)	330, 342, 485, 760, 1910
RseI	CAYNNNNRTG	6 cut(s)	255, 288, 421, 499, 1028, 1554
SacI	GAGCTC	1 cut(s)	226
SaqAI	TTAA	4 cut(s)	60, 915, 926, 1124
SatI	GCNGC	6 cut(s)	863, 1284, 1506, 1587, 1692, 1902
Sau96I	GGNCC	9 cut(s)	173, 323, 504, 638, 908, 1115, 1849, 2025, 2026
ScaI	AGTACT	1 cut(s)	1911
ScrFI	CCNGG	9 cut(s)	137, 191, 192, 508, 567, 1100, 1157, 1176, 1639
SduI	GDGCHC	3 cut(s)	115, 226, 2029
SexAI	ACCWGGT	1 cut(s)	1098
SfaNI	GCATC	5 cut(s)	259, 988, 1144, 1342, 1731
SinI	GGWCC	4 cut(s)	173, 504, 638, 1115
SmaI	CCCGGG	1 cut(s)	192
SmiMI	CAYNNNNRTG	6 cut(s)	255, 288, 421, 499, 1028, 1554
SmlI	CTYRAG	1 cut(s)	1717
SmoI	CTYRAG	1 cut(s)	1717
SphI	GCATGC	1 cut(s)	1737
SsiI	CCGC	4 cut(s)	47, 524, 947, 1694
SspMI	CTAG	2 cut(s)	1416, 1905
SstI	GAGCTC	1 cut(s)	226
StyD4I	CCNGG	9 cut(s)	135, 189, 190, 506, 565, 1098, 1155, 1174, 1637
StyI	CCWWGG	1 cut(s)	436
TaaI	ACNGT	5 cut(s)	149, 842, 856, 1318, 1982
TaiI	ACGT	4 cut(s)	34, 259, 817, 1491
TaqI	TCGA	4 cut(s)	242, 318, 408, 1298
TatI	WGTACW	1 cut(s)	1909
TfiI	GAWTC	4 cut(s)	25, 990, 1151, 1325
Tru1I	TTAA	4 cut(s)	60, 915, 926, 1124
Tru9I	TTAA	4 cut(s)	60, 915, 926, 1124
TscAI	CASTG	4 cut(s)	96, 847, 1433, 1966
TseFI	GTSAC	3 cut(s)	445, 1199, 1463
TseI	GCWGC	6 cut(s)	862, 1283, 1505, 1586, 1691, 1901
Tsp45I	GTSAC	3 cut(s)	445, 1199, 1463
TspGWI	ACGGA	3 cut(s)	433, 1715, 1878
TspMI	CCCGGG	1 cut(s)	190
TspRI	CASTG	4 cut(s)	96, 847, 1433, 1966
Tth111I	GACNNNGTC	1 cut(s)	1469
Van91I	CCANNNNNTGG	2 cut(s)	1162, 2053
VpaK11BI	GGWCC	4 cut(s)	173, 504, 638, 1115
XapI	RAATTY	1 cut(s)	6
XceI	RCATGY	1 cut(s)	1737
XcmI	CCANNNNNNNNNTGG	2 cut(s)	293, 573
XmaI	CCCGGG	1 cut(s)	190
XspI	CTAG	2 cut(s)	1416, 1905
ZrmI	AGTACT	1 cut(s)	1911
Zsp2I	ATGCAT	1 cut(s)	905

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.