Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002898

Physical Location & Seq

Genomic Coordinates Reverse (-)

24939739 .. 24952460

Gene Structure 12722 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1237000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGTGAATGAGATTGTTAATGCTCAAACATTTAACCCAGGCCGTGCTGTTTTACCCCAGGTCAAAGTAAAAGAAACTGAAGATGTGACTGAAGAGGCAGTGACAAATACAGTAAGAAGGGGTATAAGTTACCATTCAACACTTCAAGCCCATGATGGACATTGGCCCGGGGACTATGGAGGTCCTATGTTTCTACTTCCTGGCCTGGTGATTACTTTGACTATCACTGGGGCATTAAATGCAGTTTTATCAGATGAGCATAAGAAGGAGATCTGTCGATACCTTTACAATCATCAGAACAAAGATGGTGGGTGGGGATTGCACATCGAGGGCCCGAGTACTATGTTTGGTAGTGTCTTGACTTATGTTACTTTAAGGTTGCTTGGTGAAGGAGCTAAAGATGGACAAGGAGCCATGCAGAGTGGGCGTAGATGGATTCTGGACCATGGTGGTGCTACTGCAATTACGTCGTGGGGGAAAATGTGGTTATCAGTACTTGGAGCATTTGAATGGTCTGGCAACAATCCCCTGCCCCCTGAGATATGGCTTCTTCCATACATGCTCCCTATCCATCCAGGAAGGATGTGGTGTCACTGCCGGATGGTCTATCTGCCAATGTCTTACCTTTATGGGAAGAGGTTTGTTGGCCCAATCACACCGACAGTTTTGTCTTTGAGGAAAGAACTTTTTAATGTCCCCTACCATGAAATAGATTGGAATGAGGCACGCAACCAGTGTGCAAAGGAAGATTTGTACTATCCACACCCCCTCGTACAGGATATACTTTGGTCCTCTCTTCATATGGCTGTTGAGCCAATCTTGCTGCGTTGGCCTGGGAAGAAGTTGAGAGAAAAGGCTCTTCGCACTGCAATTGAGCACATACACTATGAAGATGAGAACACTCGTTATATTTGCATTGGACCTGTGAATAAGGTTTTAAATATGCTTTGCTGTTGGGCAGAAGATCCGAATTCAGAGGCTTTCAAGTTACATATTCCAAGAATCTATGATTACCTATGGATTGCTGAAGATGGCATGAAAATGCAGGGGTATAATGGAAGCCAGGCATGGGACACTTCTTTTTCTGTTCAAGCAATTATCGCAACCAACCTTATAGAAGAATTTGGTTCAATTCTAAGAAAGGGACATCAATACATAAAAGATTCACAGGTGGTTTTAGAAGATTGTCCCGGTGATCTTGACTATTGGTATCGCCACATTTCAAAGGGTGCTTGGCCTTTCTCAACTGCTGATCATGGATGGCCCATCTCAGACTGCACTGCTGAAGGATTGAAAGCTGTTCTATCATTGTCCAAAATTCCATCAGAAATTGTTGGTGAACCACTAGATACAAAGCGGCTTTATGATGCTGTCAATGTCATACTCTCATTACAGAATGGTGATGGTGGCTTTGCAACATATGAGCTCACAAGATCGTACAGTTGGTTGGAGTTGATCAACCCTGCTGAAACCTTCGGTGACATTGTAATTGATTATCCGTATGTTGAATGTACCTCAGCTTCTATTCAAGCTCTGGTATCATTTAAGAAATTATATCCTAGTCATCGGCGAGAAGAAATAGAAAGCTGTATCAAAAAAGCTGTTGAGTTTATTGAAAAGATACAAGCATCTGATGGTTCATGGTATGGTTCTTGGGGGGTTTGCTTCACTTATGGTACATGGTTTGGCGTAAAAGGGTTGGTGGCTGCTGGAAAAAATTATAACAATAGTTCCAGCATCCGGAAAGCTTGTGATTTTCTGCTATCAAAACAACTTGCTTCTGGTGGCTGGTCAGAGAGTTATCTATCATGTCAAAACAAGGTGTACACAAATCTTGATGGTGATAGGTCTCATGTGGTTAATACTGGATGGGCCATGTTGGCTCTTATTGATGCTGGCCAGGCTGAGAGAGACCCAACACCGTTGCACCGTGCAGCTAGACTTTTGATAAATTCTCAAATGGAGAATGGAGACTTTCCTCAAGAGGAAATCATGGGTGTCTTCAACCGGAACTGTATGATCACGTATGCTGCATACCGGAATATCTTCCCCATCTGGGCATTGGGTGAATACCGCTGTCGTGTCTTGCAGGCTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009555 GO:0009657 GO:0009668 GO:0009987 GO:0010027 GO:0010685 GO:0010686 GO:0016043 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0016871 GO:0019742 GO:0019745 GO:0031559 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048856 GO:0061024 GO:0071704 GO:0071840 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110

Pfam Domains

Protein Families

Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

700

Amino Acids

78.96

Weight (kDa)

5.92

Isoelectric Point (pI)

45.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	44 - 340	1.9e-38	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	354 - 692	5.1e-51	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07050 AT2G07050
fragaria_vesca	FvH4_4g05990 FvH4_4g05990 FvH4_4g05990 FvH4_4g05990 FvH4_6g36510 FvH4_6g36510
malus_domestica	MD00G1130000.v1.1 MD00G1220900.v1.1 MD13G1232100.v1.1 MD16G1237000.v1.1 MD17G1159900.v1.1 MD17G1245900.v1.1 MD17G1246400.v1.1
prunus_persica	Prupe.1G063400_v2.0.a1 Prupe.3G026100_v2.0.a1 Prupe.3G026100_v2.0.a1
pyrus_communis	pycom09g08550
rosa_chinensis	RchiOBHm_Chr2g0148711 RchiOBHm_Chr2g0148821 RchiOBHm_Chr2g0148951 RchiOBHm_Chr4g0397221
rosa_laevigata	RLG00000009438 RLG00000020359
rosa_multiflora	Rmu_sc0001796.1_g000004 Rmu_sc0002312.1_g000065 Rmu_sc0008532.1_g000008 Rmu_sc0017689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098550 Rroxscaffold_5G00342160
rosa_rugosa	Rorug03G0225900 Rorug03G0343900 Rorug03G0343900
rosa_samantha	Rh2AG467200 Rh2DG488900
rosa_wichuraiana	Rw2G038090 Rw4G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1722
AasI	GACNNNNNNGTC	1 cut(s)	667
AccIII	TCCGGA	1 cut(s)	1740
AciI	CCGC	2 cut(s)	1357, 2074
AclWI	GGATC	1 cut(s)	959
AcoI	YGGCCR	1 cut(s)	1897
AcsI	RAATTY	4 cut(s)	970, 1121, 1317, 1951
AcuI	CTGAAG	4 cut(s)	99, 111, 1047, 1305
AfaI	GTAC	8 cut(s)	340, 495, 755, 774, 1439, 1513, 1678, 1826
AfiI	CCNNNNNNNGG	4 cut(s)	775, 1069, 1740, 2056
AjnI	CCWGG	8 cut(s)	37, 57, 199, 204, 574, 832, 1062, 1899
AluBI	AGCT	9 cut(s)	395, 1298, 1426, 1520, 1532, 1587, 1601, 1748, 1937
AluI	AGCT	9 cut(s)	395, 1298, 1426, 1520, 1532, 1587, 1601, 1748, 1937
Alw21I	GWGCWC	2 cut(s)	879, 1428
Alw26I	GTCTC	3 cut(s)	1854, 1905, 1965
AlwI	GGATC	1 cut(s)	959
Ama87I	CYCGRG	2 cut(s)	167, 334
Aor13HI	TCCGGA	1 cut(s)	1740
ApaI	GGGCCC	1 cut(s)	335
ApeKI	GCWGC	4 cut(s)	823, 1706, 1934, 2030
ApoI	RAATTY	4 cut(s)	970, 1121, 1317, 1951
Asp700I	GAANNNNTTC	1 cut(s)	2045
AsuC2I	CCSGG	3 cut(s)	168, 169, 1191
AsuHPI	GGTGA	9 cut(s)	16, 220, 398, 1205, 1349, 1412, 1490, 1853, 2078
AvaI	CYCGRG	2 cut(s)	167, 334
AvaII	GGWCC	4 cut(s)	182, 442, 789, 920
BaeGI	GKGCMC	1 cut(s)	335
BalI	TGGCCA	1 cut(s)	1899
BanII	GRGCYC	2 cut(s)	335, 1428
BarI	GAAGNNNNNNTAC	2 cut(s)	1504, 1536
BbsI	GAAGAC	1 cut(s)	1993
Bbv12I	GWGCWC	2 cut(s)	879, 1428
BbvCI	CCTCAGC	1 cut(s)	1516
BbvI	GCAGC	4 cut(s)	810, 1693, 1946, 2017
BceAI	ACGGC	1 cut(s)	27
BcgI	CGANNNNNNTGC	2 cut(s)	450, 484
BciT130I	CCWGG	8 cut(s)	39, 59, 201, 206, 576, 834, 1064, 1901
BclI	TGATCA	3 cut(s)	1252, 1455, 2019
BcnI	CCSGG	3 cut(s)	168, 169, 1191
BcoDI	GTCTC	3 cut(s)	1854, 1905, 1965
BfaI	CTAG	3 cut(s)	1346, 1560, 1938
BglI	GCCNNNNNGGC	1 cut(s)	1880
BglII	AGATCT	1 cut(s)	270
BisI	GCNGC	5 cut(s)	824, 1358, 1707, 1935, 2031
BlsI	GCNGC	5 cut(s)	825, 1359, 1708, 1936, 2032
BmcAI	AGTACT	2 cut(s)	340, 495
Bme18I	GGWCC	4 cut(s)	182, 442, 789, 920
BmeT110I	CYCGRG	2 cut(s)	167, 334
BmiI	GGNNCC	2 cut(s)	333, 412
BmrI	ACTGGG	1 cut(s)	237
BmsI	GCATC	4 cut(s)	1357, 1637, 1746, 1882
BmuI	ACTGGG	1 cut(s)	237
BpiI	GAAGAC	1 cut(s)	1993
Bpu10I	CCTNAGC	1 cut(s)	1516
BpuEI	CTTGAG	1 cut(s)	1965
BpuMI	CCSGG	3 cut(s)	168, 169, 1191
BsaAI	YACGTR	1 cut(s)	2025
BsaI	GGTCTC	2 cut(s)	1854, 1905
BsaJI	CCNNGG	6 cut(s)	37, 57, 167, 168, 445, 833
BsaWI	WCCGGW	3 cut(s)	1740, 2007, 2037
Bsc4I	CCNNNNNNNGG	4 cut(s)	775, 1069, 1740, 2056
Bse1I	ACTGG	3 cut(s)	232, 733, 1870
BseAI	TCCGGA	1 cut(s)	1740
BseBI	CCWGG	8 cut(s)	39, 59, 201, 206, 576, 834, 1064, 1901
BseDI	CCNNGG	6 cut(s)	37, 57, 167, 168, 445, 833
BseGI	GGATG	6 cut(s)	571, 588, 606, 1265, 1737, 1874
BseLI	CCNNNNNNNGG	4 cut(s)	775, 1069, 1740, 2056
BseMII	CTCAG	4 cut(s)	528, 1284, 1530, 1896
BseNI	ACTGG	3 cut(s)	232, 733, 1870
BseSI	GKGCMC	1 cut(s)	335
BseXI	GCAGC	4 cut(s)	810, 1693, 1946, 2017
BsgI	GTGCAG	2 cut(s)	1261, 1953
BsiHKAI	GWGCWC	2 cut(s)	879, 1428
BsiHKCI	CYCGRG	2 cut(s)	167, 334
BsiSI	CCGG	6 cut(s)	168, 598, 1191, 1741, 2008, 2038
BslFI	GGGAC	5 cut(s)	185, 680, 1085, 1158, 1173
BslI	CCNNNNNNNGG	4 cut(s)	775, 1069, 1740, 2056
BsmAI	GTCTC	3 cut(s)	1854, 1905, 1965
BsmFI	GGGAC	5 cut(s)	185, 680, 1085, 1158, 1173
Bso31I	GGTCTC	2 cut(s)	1854, 1905
BsoBI	CYCGRG	2 cut(s)	167, 334
Bsp120I	GGGCCC	1 cut(s)	331
Bsp1286I	GDGCHC	3 cut(s)	335, 879, 1428
Bsp13I	TCCGGA	1 cut(s)	1740
Bsp1407I	TGTACA	1 cut(s)	1824
Bsp143I	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
Bsp19I	CCATGG	1 cut(s)	445
BspACI	CCGC	2 cut(s)	1357, 2074
BspCNI	CTCAG	4 cut(s)	529, 1283, 1529, 1897
BspEI	TCCGGA	1 cut(s)	1740
BspLI	GGNNCC	2 cut(s)	333, 412
BspPI	GGATC	1 cut(s)	959
BspQI	GCTCTTC	1 cut(s)	864
BspTNI	GGTCTC	2 cut(s)	1854, 1905
BsrGI	TGTACA	1 cut(s)	1824
BsrI	ACTGG	3 cut(s)	232, 733, 1870
BssECI	CCNNGG	6 cut(s)	37, 57, 167, 168, 445, 833
BssMI	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
BssT1I	CCWWGG	1 cut(s)	445
Bst2UI	CCWGG	8 cut(s)	39, 59, 201, 206, 576, 834, 1064, 1901
Bst4CI	ACNGT	6 cut(s)	112, 664, 1442, 1923, 1931, 2015
Bst6I	CTCTTC	4 cut(s)	87, 629, 801, 864
BstAPI	GCANNNNNTGC	1 cut(s)	239
BstAUI	TGTACA	1 cut(s)	1824
BstBAI	YACGTR	1 cut(s)	2025
BstC8I	GCNNGC	3 cut(s)	727, 1897, 2091
BstDEI	CTNAG	5 cut(s)	537, 1136, 1270, 1516, 1905
BstDSI	CCRYGG	1 cut(s)	445
BstENI	CCTNNNNNAGG	1 cut(s)	773
BstF5I	GGATG	6 cut(s)	571, 588, 606, 1265, 1737, 1874
BstKTI	GATC	7 cut(s)	273, 967, 1198, 1255, 1436, 1458, 2022
BstMAI	GTCTC	3 cut(s)	1854, 1905, 1965
BstMBI	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
BstMWI	GCNNNNNNNGC	7 cut(s)	239, 424, 820, 829, 1100, 1880, 1901
BstNI	CCWGG	8 cut(s)	39, 59, 201, 206, 576, 834, 1064, 1901
BstNSI	RCATGY	1 cut(s)	562
BstSLI	GKGCMC	1 cut(s)	335
BstV1I	GCAGC	4 cut(s)	810, 1693, 1946, 2017
BstV2I	GAAGAC	1 cut(s)	1993
BstX2I	RGATCY	2 cut(s)	270, 964
BstYI	RGATCY	2 cut(s)	270, 964
BtgI	CCRYGG	1 cut(s)	445
BtsCI	GGATG	6 cut(s)	571, 588, 606, 1265, 1737, 1874
BtsI	GCAGTG	4 cut(s)	105, 592, 864, 1278
BtsIMutI	CAGTG	6 cut(s)	105, 225, 592, 740, 864, 1278
Cac8I	GCNNGC	3 cut(s)	727, 1897, 2091
Cfr9I	CCCGGG	1 cut(s)	167
Csp6I	GTAC	8 cut(s)	339, 494, 754, 773, 1438, 1512, 1677, 1825
CspCI	CAANNNNNGTGG	2 cut(s)	289, 324
CviQI	GTAC	8 cut(s)	339, 494, 754, 773, 1438, 1512, 1677, 1825
DdeI	CTNAG	5 cut(s)	537, 1136, 1270, 1516, 1905
DpnI	GATC	7 cut(s)	272, 966, 1197, 1254, 1435, 1457, 2021
DpnII	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
DraI	TTTAAA	1 cut(s)	939
DrdI	GACNNNNNNGTC	1 cut(s)	667
DseDI	GACNNNNNNGTC	1 cut(s)	667
EaeI	YGGCCR	1 cut(s)	1897
Eam1104I	CTCTTC	4 cut(s)	87, 629, 801, 864
EarI	CTCTTC	4 cut(s)	87, 629, 801, 864
Ecl136II	GAGCTC	1 cut(s)	1426
Eco130I	CCWWGG	1 cut(s)	445
Eco24I	GRGCYC	2 cut(s)	335, 1428
Eco31I	GGTCTC	2 cut(s)	1854, 1905
Eco47I	GGWCC	4 cut(s)	182, 442, 789, 920
Eco53kI	GAGCTC	1 cut(s)	1426
Eco57I	CTGAAG	4 cut(s)	99, 111, 1047, 1305
Eco88I	CYCGRG	2 cut(s)	167, 334
EcoICRI	GAGCTC	1 cut(s)	1426
EcoNI	CCTNNNNNAGG	1 cut(s)	773
EcoO109I	RGGNCCY	2 cut(s)	182, 331
EcoRI	GAATTC	1 cut(s)	970
EcoRII	CCWGG	8 cut(s)	37, 57, 199, 204, 574, 832, 1062, 1899
EcoT14I	CCWWGG	1 cut(s)	445
EcoT38I	GRGCYC	2 cut(s)	335, 1428
ErhI	CCWWGG	1 cut(s)	445
FaqI	GGGAC	5 cut(s)	185, 680, 1085, 1158, 1173
FauNDI	CATATG	2 cut(s)	801, 1420
FbaI	TGATCA	3 cut(s)	1252, 1455, 2019
Fnu4HI	GCNGC	5 cut(s)	824, 1358, 1707, 1935, 2031
FokI	GGATG	6 cut(s)	558, 595, 613, 1272, 1724, 1881
FriOI	GRGCYC	2 cut(s)	335, 1428
Fsp4HI	GCNGC	5 cut(s)	824, 1358, 1707, 1935, 2031
FspBI	CTAG	3 cut(s)	1346, 1560, 1938
GluI	GCNGC	5 cut(s)	824, 1358, 1707, 1935, 2031
HapII	CCGG	6 cut(s)	168, 598, 1191, 1741, 2008, 2038
HindIII	AAGCTT	1 cut(s)	1746
HinfI	GANTC	3 cut(s)	436, 1002, 1163
HpaII	CCGG	6 cut(s)	168, 598, 1191, 1741, 2008, 2038
HphI	GGTGA	9 cut(s)	16, 220, 398, 1205, 1349, 1412, 1490, 1853, 2078
Hpy166II	GTNNAC	3 cut(s)	1340, 1825, 1827
Hpy188I	TCNGA	8 cut(s)	253, 297, 969, 976, 1273, 1327, 1633, 1795
Hpy188III	TCNNGA	7 cut(s)	358, 440, 1199, 1741, 1835, 1982, 2097
Hpy8I	GTNNAC	3 cut(s)	1340, 1825, 1827
Hpy99I	CGWCG	1 cut(s)	472
HpyAV	CCTTC	6 cut(s)	111, 259, 383, 573, 1280, 1483
HpyCH4III	ACNGT	6 cut(s)	112, 664, 1442, 1923, 1931, 2015
HpyCH4IV	ACGT	2 cut(s)	467, 2024
HpyF10VI	GCNNNNNNNGC	7 cut(s)	239, 424, 820, 829, 1100, 1880, 1901
HpyF3I	CTNAG	5 cut(s)	537, 1136, 1270, 1516, 1905
HpySE526I	ACGT	2 cut(s)	467, 2024
Kpn2I	TCCGGA	1 cut(s)	1740
Ksp22I	TGATCA	3 cut(s)	1252, 1455, 2019
Kzo9I	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
LguI	GCTCTTC	1 cut(s)	864
LmnI	GCTCC	4 cut(s)	392, 410, 500, 567
Lsp1109I	GCAGC	4 cut(s)	810, 1693, 1946, 2017
LweI	GCATC	4 cut(s)	1357, 1637, 1746, 1882
MaeI	CTAG	3 cut(s)	1346, 1560, 1938
MaeII	ACGT	2 cut(s)	467, 2024
MaeIII	GTNAC	7 cut(s)	85, 100, 128, 367, 590, 987, 1478
MalI	GATC	7 cut(s)	272, 966, 1197, 1254, 1435, 1457, 2021
MboI	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
MfeI	CAATTG	1 cut(s)	870
MflI	RGATCY	2 cut(s)	270, 964
MhlI	GDGCHC	3 cut(s)	335, 879, 1428
MlsI	TGGCCA	1 cut(s)	1899
MluNI	TGGCCA	1 cut(s)	1899
MmeI	TCCRAC	1 cut(s)	1428
Mox20I	TGGCCA	1 cut(s)	1899
MroI	TCCGGA	1 cut(s)	1740
MroXI	GAANNNNTTC	1 cut(s)	2045
MscI	TGGCCA	1 cut(s)	1899
MseI	TTAA	8 cut(s)	18, 33, 236, 374, 690, 938, 1545, 1860
MslI	CAYNNNNRTG	3 cut(s)	450, 508, 1040
Msp20I	TGGCCA	1 cut(s)	1899
MspA1I	CMGCKG	1 cut(s)	2076
MspI	CCGG	6 cut(s)	168, 598, 1191, 1741, 2008, 2038
MunI	CAATTG	1 cut(s)	870
MvaI	CCWGG	8 cut(s)	39, 59, 201, 206, 576, 834, 1064, 1901
MwoI	GCNNNNNNNGC	7 cut(s)	239, 424, 820, 829, 1100, 1880, 1901
NciI	CCSGG	3 cut(s)	168, 169, 1191
NcoI	CCATGG	1 cut(s)	445
NdeI	CATATG	2 cut(s)	801, 1420
NdeII	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
NlaIV	GGNNCC	2 cut(s)	333, 412
NmuCI	GTSAC	4 cut(s)	85, 100, 590, 1478
NspI	RCATGY	1 cut(s)	562
PciSI	GCTCTTC	1 cut(s)	864
PdmI	GAANNNNTTC	1 cut(s)	2045
PfeI	GAWTC	3 cut(s)	436, 1002, 1163
PfoI	TCCNGGA	1 cut(s)	574
PkrI	GCNGC	5 cut(s)	825, 1359, 1708, 1936, 2032
Ppu21I	YACGTR	1 cut(s)	2025
PpuMI	RGGWCCY	1 cut(s)	182
PsiI	TTATAA	1 cut(s)	1722
Psp124BI	GAGCTC	1 cut(s)	1428
Psp5II	RGGWCCY	1 cut(s)	182
Psp6I	CCWGG	8 cut(s)	37, 57, 199, 204, 574, 832, 1062, 1899
PspGI	CCWGG	8 cut(s)	37, 57, 199, 204, 574, 832, 1062, 1899
PspN4I	GGNNCC	2 cut(s)	333, 412
PspOMI	GGGCCC	1 cut(s)	331
PspPPI	RGGWCCY	1 cut(s)	182
PsuI	RGATCY	2 cut(s)	270, 964
RsaI	GTAC	8 cut(s)	340, 495, 755, 774, 1439, 1513, 1678, 1826
RsaNI	GTAC	8 cut(s)	339, 494, 754, 773, 1438, 1512, 1677, 1825
RseI	CAYNNNNRTG	3 cut(s)	450, 508, 1040
SacI	GAGCTC	1 cut(s)	1428
SapI	GCTCTTC	1 cut(s)	864
SaqAI	TTAA	8 cut(s)	18, 33, 236, 374, 690, 938, 1545, 1860
SatI	GCNGC	5 cut(s)	824, 1358, 1707, 1935, 2031
Sau3AI	GATC	7 cut(s)	270, 964, 1195, 1252, 1433, 1455, 2019
ScaI	AGTACT	2 cut(s)	340, 495
SduI	GDGCHC	3 cut(s)	335, 879, 1428
SfaNI	GCATC	4 cut(s)	1357, 1637, 1746, 1882
SinI	GGWCC	4 cut(s)	182, 442, 789, 920
SmaI	CCCGGG	1 cut(s)	169
SmiMI	CAYNNNNRTG	3 cut(s)	450, 508, 1040
SmlI	CTYRAG	1 cut(s)	1980
SmoI	CTYRAG	1 cut(s)	1980
SsiI	CCGC	2 cut(s)	1357, 2074
SspMI	CTAG	3 cut(s)	1346, 1560, 1938
SstI	GAGCTC	1 cut(s)	1428
StyI	CCWWGG	1 cut(s)	445
TaaI	ACNGT	6 cut(s)	112, 664, 1442, 1923, 1931, 2015
TaiI	ACGT	2 cut(s)	470, 2027
TaqI	TCGA	2 cut(s)	277, 327
TatI	WGTACW	4 cut(s)	338, 493, 753, 1824
TauI	GCSGC	1 cut(s)	1360
TfiI	GAWTC	3 cut(s)	436, 1002, 1163
Tru1I	TTAA	8 cut(s)	18, 33, 236, 374, 690, 938, 1545, 1860
Tru9I	TTAA	8 cut(s)	18, 33, 236, 374, 690, 938, 1545, 1860
TscAI	CASTG	6 cut(s)	105, 232, 599, 740, 871, 1285
TseFI	GTSAC	4 cut(s)	85, 100, 590, 1478
TseI	GCWGC	4 cut(s)	823, 1706, 1934, 2030
Tsp45I	GTSAC	4 cut(s)	85, 100, 590, 1478
TspDTI	ATGAA	5 cut(s)	720, 788, 903, 1052, 1629
TspGWI	ACGGA	1 cut(s)	1488
TspMI	CCCGGG	1 cut(s)	167
TspRI	CASTG	6 cut(s)	105, 232, 599, 740, 871, 1285
VpaK11BI	GGWCC	4 cut(s)	182, 442, 789, 920
XagI	CCTNNNNNAGG	1 cut(s)	773
XapI	RAATTY	4 cut(s)	970, 1121, 1317, 1951
XceI	RCATGY	1 cut(s)	562
XcmI	CCANNNNNNNNNTGG	2 cut(s)	582, 2059
XmaI	CCCGGG	1 cut(s)	167
XmnI	GAANNNNTTC	1 cut(s)	2045
XspI	CTAG	3 cut(s)	1346, 1560, 1938
ZrmI	AGTACT	2 cut(s)	340, 495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.