Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002898

Physical Location & Seq

Genomic Coordinates Reverse (-)

23054954 .. 23067382

Gene Structure 12429 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1232100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGTGGAAGCTGAAGATTGCGGAGGGAGGGAGTCCATGGCTGCGTACACTCAAAAACAATGTGGGCAGGCAAGTCTGGGAGTTCGATCCCAAGCTCGGTACCCCCGAAGAGCTCCTCGAGATCGAGAGAGCTCGCGAGAACTACAGTAATAATCGGTTCGTTAAGAAGCACAGCTCCGATTTGCTCATGCGCATTCAGTTTTCCAAGGAGAACCCAGTTCCTGCTGTTTTACCCCAGGTCAAAGTAAAAGACACAGAAGATGTGACTGAGAAGACAGTGACAAATACATTAAAAAGGGGTATAAGTTACCATTCAACACTTCAAGCCCATGATGGACATTGGCCTGGGGACTATGGAGGTCCTATGTTTCTACTCCCGGGCCTGGTAATTGCTTTGTCTATCACTGGGGCATTGAATGCAGTCTTATCAGATGAGCATAAGAAGGAGATCTGTCGATACCTCTACAATCATCAGAATAAAGATGGTGGGTGGGGCTTGCACATTGAGGGCCCAAGTACTATGTTTGGCAGTGTCTTGACATATGTTACTTTAAGGTTGCTTGGTGAAGGAAATAAAGATGGACAAGGAGCCATGGAGAGTGGGCGTAGGTGGATTCTGGACCATGGTGGTGCTACTGCAATTACATCGTGGGGGAAAATGTGGTTATCAGTACTTGGAGCATTTGAATGGTCTGGCAATAATCCCCTGCCCCCTGAGATATGGCTTCTTCCATATATGCTCCCAATTCATCCAGGAAGGATGTGGTGTCACTGCCGCATGGTCTATTTGCCAATGTCATACCTATATGGGAAGAGGTTTGTTGGCCCAATCACACCAACAGTTTTGTCTTTGAGGAAAGAACTTTTTAATGTCCCCTATCATGAAATAGATTGGAATGAGGCACGCAACCAGTGTGCAAAGGAAGATTTGTACTATCCACACCCCCTTGTACAAGATATACTTTGGTCTTCTCTTCATATGGCTGTTGAACCAATCTTAATGCATTGGCCTGGGAAGAAGTTGAGAGAAAAGGCTCTTCGCACTGCAATTGAGCACATACATTATGAAGATGAGAATACTCGTTATATTTGCATTGGACCTGTGAATAAGGTTTTAAATATGCTTTGCTGTTGGGCAGAAGATCCGAATTCAGAGGCTTTCAAGTTACATATTCCAAGAATCTATGATTACCTATGGATTGCTGAAGATGGCATGAAAATGCAGGGGTATAATGGAAGCCAGGCTTGGGACACTTCTTTTTCTGTTCAAGCAATTATCGCAACCAACCTTATAGAAGAATTTGGTTCAACTCTAAGAAAGGGACATCAATACATAAAAGATTCACAGGTGGTTTTAGAAGATTGCCCCGGTGATCTTGACTATTGGTATCGCCACATTTCAAAGGGTGCTTGGCCTTTCTCAACTGCTGATCATGGATGGCCCATCTCAGACTGCACAGCTGAAGGATTGAAAGCTGTTCTATCATTATCAAAAATTCCATCTGAAATTGTTGGTGAACCACTAGATGCAAACCGGCTTTACGATGCTGTCAATGTCATACTCTCATTACAGAATAATGACGGTGGTTTTGCAACATATGAGCTCACAAGATCTTACAGTTGGTTGGAGTTGATCAACCCTGCTGAAACCTTTGGTGACATTGTAATCGATTATCCTTATGTTGAATGCACCTCAGCTTCTATTCAAGCTCTGGAATCATTTAAGAAATTATATCCTTGTCATCGGCGAGAAGAAATAGAACACTGTATCAAAAGAGCTGTTGAGTTTATTGAAAAGATACAAGCATCTGATGGTTCATGGTATGGTTCGTGGGGGGTTTGCTTCACTTATGGTACATGGTTTGGGGTAAAAGGGTTGGTGGCTGCTGGAAGGAACTACAACAATAGTTCTAGCATTCGGAAAGCTTGTGATTTTCTGCTATCAAAACAACTTACTTCTGGTGGGTGGTCAGAGAGTTATCTATCATGTCAAAACAAGGTGTACACAAATCTTGAGGGTGATAGGTCTCACGTGGTCAATACTGGATGGGCCATGTTGGCTCTTATTGATGGTGGCCAGGCTGAGAGAGACCCTACACCGTTGCACCGTGCGGCTAGATTTTTGATAAATTCTCAAATAGAGAATGGAGATTTTCCTCAAGAGGAAATTATGGGTGTCTTCAACAGGAACTGTATGATCACGTATGCTGCATACCGGAACATATTCCCTATCTGGGCATTGGGAGAATACCGCTGTCGAGTGTTGCAGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009555 GO:0009657 GO:0009668 GO:0009987 GO:0010027 GO:0010685 GO:0010686 GO:0016043 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0016871 GO:0019742 GO:0019745 GO:0031559 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048856 GO:0061024 GO:0071704 GO:0071840 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110

Pfam Domains

Protein Families

Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

758

Amino Acids

86.14

Weight (kDa)

6.08

Isoelectric Point (pI)

44.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	103 - 399	4.7e-38	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	413 - 751	1.4e-51	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07050 AT2G07050
fragaria_vesca	FvH4_4g05990 FvH4_4g05990 FvH4_4g05990 FvH4_4g05990 FvH4_6g36510 FvH4_6g36510
malus_domestica	MD00G1130000.v1.1 MD00G1220900.v1.1 MD13G1232100.v1.1 MD16G1237000.v1.1 MD17G1159900.v1.1 MD17G1245900.v1.1 MD17G1246400.v1.1
prunus_persica	Prupe.1G063400_v2.0.a1 Prupe.3G026100_v2.0.a1 Prupe.3G026100_v2.0.a1
pyrus_communis	pycom09g08550
rosa_chinensis	RchiOBHm_Chr2g0148711 RchiOBHm_Chr2g0148821 RchiOBHm_Chr2g0148951 RchiOBHm_Chr4g0397221
rosa_laevigata	RLG00000009438 RLG00000020359
rosa_multiflora	Rmu_sc0001796.1_g000004 Rmu_sc0002312.1_g000065 Rmu_sc0008532.1_g000008 Rmu_sc0017689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00098550 Rroxscaffold_5G00342160
rosa_rugosa	Rorug03G0225900 Rorug03G0343900 Rorug03G0343900
rosa_samantha	Rh2AG467200 Rh2DG488900
rosa_wichuraiana	Rw2G038090 Rw4G006310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	191
Acc65I	GGTACC	1 cut(s)	98
AccB1I	GGYRCC	1 cut(s)	98
AccII	CGCG	1 cut(s)	135
AciI	CCGC	4 cut(s)	20, 775, 2111, 2251
AclWI	GGATC	2 cut(s)	80, 1136
AcoI	YGGCCR	1 cut(s)	2074
AcsI	RAATTY	4 cut(s)	1147, 1298, 1494, 2128
AcuI	CTGAAG	3 cut(s)	32, 1224, 1482
AcvI	CACGTG	1 cut(s)	2032
AfaI	GTAC	8 cut(s)	46, 100, 517, 672, 932, 951, 1855, 2003
AfiI	CCNNNNNNNGG	4 cut(s)	95, 382, 1246, 2233
AjnI	CCWGG	7 cut(s)	234, 343, 381, 751, 1009, 1239, 2076
AjuI	GAANNNNNNNTTGG	2 cut(s)	1228, 1260
Alw21I	GWGCWC	4 cut(s)	114, 133, 1056, 1605
Alw26I	GTCTC	2 cut(s)	2031, 2082
AlwI	GGATC	2 cut(s)	80, 1136
AlwNI	CAGNNNCTG	2 cut(s)	221, 2190
Ama87I	CYCGRG	2 cut(s)	116, 376
AoxI	GGCC	9 cut(s)	341, 379, 508, 823, 1007, 1412, 1439, 2049, 2074
ApaI	GGGCCC	1 cut(s)	512
ApeKI	GCWGC	3 cut(s)	40, 1883, 2207
ApoI	RAATTY	4 cut(s)	1147, 1298, 1494, 2128
ArsI	GACNNNNNNTTYG	2 cut(s)	1571, 1603
Asp700I	GAANNNNTTC	1 cut(s)	2222
Asp718I	GGTACC	1 cut(s)	98
AspLEI	GCGC	1 cut(s)	192
AspS9I	GGNCC	9 cut(s)	359, 379, 508, 509, 619, 824, 1097, 1440, 2049
AsuC2I	CCSGG	3 cut(s)	377, 378, 1368
AsuHPI	GGTGA	5 cut(s)	575, 1382, 1526, 1667, 2030
AvaI	CYCGRG	2 cut(s)	116, 376
AvaII	GGWCC	3 cut(s)	359, 619, 1097
BaeGI	GKGCMC	1 cut(s)	512
BalI	TGGCCA	1 cut(s)	2076
BanI	GGYRCC	1 cut(s)	98
BanII	GRGCYC	4 cut(s)	114, 133, 512, 1605
BbrPI	CACGTG	1 cut(s)	2032
BbsI	GAAGAC	3 cut(s)	278, 960, 2170
Bbv12I	GWGCWC	4 cut(s)	114, 133, 1056, 1605
BbvCI	CCTCAGC	1 cut(s)	1693
BbvI	GCAGC	3 cut(s)	27, 1870, 2194
BcgI	CGANNNNNNTGC	2 cut(s)	627, 661
BciT130I	CCWGG	7 cut(s)	236, 345, 383, 753, 1011, 1241, 2078
BclI	TGATCA	3 cut(s)	1429, 1632, 2196
BcnI	CCSGG	3 cut(s)	377, 378, 1368
BcoDI	GTCTC	2 cut(s)	2031, 2082
BfaI	CTAG	3 cut(s)	1523, 1911, 2115
BfmI	CTRYAG	1 cut(s)	142
BglI	GCCNNNNNGGC	1 cut(s)	2057
BglII	AGATCT	2 cut(s)	447, 1610
BisI	GCNGC	5 cut(s)	41, 775, 1884, 2112, 2208
BlsI	GCNGC	5 cut(s)	42, 776, 1885, 2113, 2209
BmcAI	AGTACT	2 cut(s)	517, 672
Bme18I	GGWCC	3 cut(s)	359, 619, 1097
BmeT110I	CYCGRG	2 cut(s)	116, 376
BmgT120I	GGNCC	9 cut(s)	359, 379, 508, 509, 619, 824, 1097, 1440, 2049
BmiI	GGNNCC	3 cut(s)	100, 510, 589
BmrI	ACTGGG	2 cut(s)	209, 414
BmsI	GCATC	3 cut(s)	1516, 1534, 1814
BmuI	ACTGGG	2 cut(s)	209, 414
BpiI	GAAGAC	3 cut(s)	278, 960, 2170
Bpu10I	CCTNAGC	1 cut(s)	1693
BpuEI	CTTGAG	2 cut(s)	2033, 2142
BpuMI	CCSGG	3 cut(s)	377, 378, 1368
Bsa29I	ATCGAT	1 cut(s)	1668
BsaAI	YACGTR	2 cut(s)	2032, 2202
BsaI	GGTCTC	2 cut(s)	2031, 2082
BsaJI	CCNNGG	9 cut(s)	35, 204, 234, 344, 376, 591, 622, 1010, 1366
BsaWI	WCCGGW	1 cut(s)	2214
Bsc4I	CCNNNNNNNGG	4 cut(s)	95, 382, 1246, 2233
Bse118I	RCCGGY	1 cut(s)	1533
Bse1I	ACTGG	4 cut(s)	215, 409, 910, 2047
BseBI	CCWGG	7 cut(s)	236, 345, 383, 753, 1011, 1241, 2078
BseCI	ATCGAT	1 cut(s)	1668
BseDI	CCNNGG	9 cut(s)	35, 204, 234, 344, 376, 591, 622, 1010, 1366
BseGI	GGATG	4 cut(s)	748, 765, 1442, 2051
BseLI	CCNNNNNNNGG	4 cut(s)	95, 382, 1246, 2233
BseMII	CTCAG	5 cut(s)	258, 705, 1461, 1707, 2073
BseNI	ACTGG	4 cut(s)	215, 409, 910, 2047
BseRI	GAGGAG	1 cut(s)	104
BseSI	GKGCMC	1 cut(s)	512
BseXI	GCAGC	3 cut(s)	27, 1870, 2194
BsgI	GTGCAG	1 cut(s)	1438
Bsh1236I	CGCG	1 cut(s)	135
BshFI	GGCC	9 cut(s)	343, 381, 510, 825, 1009, 1414, 1441, 2051, 2076
BshNI	GGYRCC	1 cut(s)	98
BshVI	ATCGAT	1 cut(s)	1668
BsiHKAI	GWGCWC	4 cut(s)	114, 133, 1056, 1605
BsiHKCI	CYCGRG	2 cut(s)	116, 376
BsiSI	CCGG	4 cut(s)	377, 1368, 1534, 2215
BslFI	GGGAC	4 cut(s)	362, 857, 1262, 1335
BslI	CCNNNNNNNGG	4 cut(s)	95, 382, 1246, 2233
BsmAI	GTCTC	2 cut(s)	2031, 2082
BsmFI	GGGAC	4 cut(s)	362, 857, 1262, 1335
BsmI	GAATGC	4 cut(s)	192, 421, 1691, 1914
BsnI	GGCC	9 cut(s)	343, 381, 510, 825, 1009, 1414, 1441, 2051, 2076
Bso31I	GGTCTC	2 cut(s)	2031, 2082
BsoBI	CYCGRG	2 cut(s)	116, 376
Bsp120I	GGGCCC	1 cut(s)	508
Bsp1286I	GDGCHC	5 cut(s)	114, 133, 512, 1056, 1605
Bsp1407I	TGTACA	2 cut(s)	949, 2001
Bsp143I	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
Bsp19I	CCATGG	3 cut(s)	35, 591, 622
Bsp68I	TCGCGA	1 cut(s)	135
BspACI	CCGC	4 cut(s)	20, 775, 2111, 2251
BspANI	GGCC	9 cut(s)	343, 381, 510, 825, 1009, 1414, 1441, 2051, 2076
BspCNI	CTCAG	5 cut(s)	259, 706, 1460, 1706, 2074
BspDI	ATCGAT	1 cut(s)	1668
BspFNI	CGCG	1 cut(s)	135
BspHI	TCATGA	1 cut(s)	880
BspLI	GGNNCC	3 cut(s)	100, 510, 589
BspPI	GGATC	2 cut(s)	80, 1136
BspQI	GCTCTTC	2 cut(s)	102, 1041
BspT107I	GGYRCC	1 cut(s)	98
BspTNI	GGTCTC	2 cut(s)	2031, 2082
BsrFI	RCCGGY	1 cut(s)	1533
BsrGI	TGTACA	2 cut(s)	949, 2001
BsrI	ACTGG	4 cut(s)	215, 409, 910, 2047
BssAI	RCCGGY	1 cut(s)	1533
BssECI	CCNNGG	9 cut(s)	35, 204, 234, 344, 376, 591, 622, 1010, 1366
BssMI	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
BssT1I	CCWWGG	4 cut(s)	35, 204, 591, 622
Bst2UI	CCWGG	7 cut(s)	236, 345, 383, 753, 1011, 1241, 2078
Bst4CI	ACNGT	9 cut(s)	146, 277, 841, 1583, 1619, 1766, 2100, 2108, 2192
Bst6I	CTCTTC	4 cut(s)	102, 806, 978, 1041
BstAPI	GCANNNNNTGC	1 cut(s)	416
BstAUI	TGTACA	2 cut(s)	949, 2001
BstBAI	YACGTR	2 cut(s)	2032, 2202
BstC8I	GCNNGC	5 cut(s)	68, 133, 497, 904, 2268
BstDEI	CTNAG	7 cut(s)	267, 714, 1313, 1447, 1693, 2082, 2271
BstDSI	CCRYGG	3 cut(s)	35, 591, 622
BstF5I	GGATG	4 cut(s)	748, 765, 1442, 2051
BstFNI	CGCG	1 cut(s)	135
BstHHI	GCGC	1 cut(s)	192
BstKTI	GATC	9 cut(s)	88, 123, 450, 1144, 1375, 1432, 1613, 1635, 2199
BstMAI	GTCTC	2 cut(s)	2031, 2082
BstMBI	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
BstMWI	GCNNNNNNNGC	3 cut(s)	416, 1277, 2057
BstNI	CCWGG	7 cut(s)	236, 345, 383, 753, 1011, 1241, 2078
BstSFI	CTRYAG	1 cut(s)	142
BstSLI	GKGCMC	1 cut(s)	512
BstUI	CGCG	1 cut(s)	135
BstV1I	GCAGC	3 cut(s)	27, 1870, 2194
BstV2I	GAAGAC	3 cut(s)	278, 960, 2170
BstX2I	RGATCY	3 cut(s)	447, 1141, 1610
BstYI	RGATCY	3 cut(s)	447, 1141, 1610
Bsu15I	ATCGAT	1 cut(s)	1668
BsuRI	GGCC	9 cut(s)	343, 381, 510, 825, 1009, 1414, 1441, 2051, 2076
BsuTUI	ATCGAT	1 cut(s)	1668
BtgI	CCRYGG	3 cut(s)	35, 591, 622
BtsCI	GGATG	4 cut(s)	748, 765, 1442, 2051
BtsI	GCAGTG	3 cut(s)	535, 769, 1041
BtsIMutI	CAGTG	7 cut(s)	282, 402, 535, 769, 917, 1041, 1762
BtuMI	TCGCGA	1 cut(s)	135
Cac8I	GCNNGC	5 cut(s)	68, 133, 497, 904, 2268
CaiI	CAGNNNCTG	2 cut(s)	221, 2190
CciI	TCATGA	1 cut(s)	880
CfoI	GCGC	1 cut(s)	192
Cfr10I	RCCGGY	1 cut(s)	1533
Cfr13I	GGNCC	9 cut(s)	359, 379, 508, 509, 619, 824, 1097, 1440, 2049
Cfr9I	CCCGGG	1 cut(s)	376
ClaI	ATCGAT	1 cut(s)	1668
Csp6I	GTAC	8 cut(s)	45, 99, 516, 671, 931, 950, 1854, 2002
CviQI	GTAC	8 cut(s)	45, 99, 516, 671, 931, 950, 1854, 2002
DdeI	CTNAG	7 cut(s)	267, 714, 1313, 1447, 1693, 2082, 2271
DpnI	GATC	9 cut(s)	87, 122, 449, 1143, 1374, 1431, 1612, 1634, 2198
DpnII	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
DraI	TTTAAA	1 cut(s)	1116
EaeI	YGGCCR	1 cut(s)	2074
Eam1104I	CTCTTC	4 cut(s)	102, 806, 978, 1041
EarI	CTCTTC	4 cut(s)	102, 806, 978, 1041
Ecl136II	GAGCTC	3 cut(s)	112, 131, 1603
Eco130I	CCWWGG	4 cut(s)	35, 204, 591, 622
Eco24I	GRGCYC	4 cut(s)	114, 133, 512, 1605
Eco31I	GGTCTC	2 cut(s)	2031, 2082
Eco47I	GGWCC	3 cut(s)	359, 619, 1097
Eco53kI	GAGCTC	3 cut(s)	112, 131, 1603
Eco57I	CTGAAG	3 cut(s)	32, 1224, 1482
Eco72I	CACGTG	1 cut(s)	2032
Eco88I	CYCGRG	2 cut(s)	116, 376
EcoICRI	GAGCTC	3 cut(s)	112, 131, 1603
EcoO109I	RGGNCCY	2 cut(s)	359, 508
EcoRI	GAATTC	1 cut(s)	1147
EcoRII	CCWGG	7 cut(s)	234, 343, 381, 751, 1009, 1239, 2076
EcoT14I	CCWWGG	4 cut(s)	35, 204, 591, 622
EcoT22I	ATGCAT	1 cut(s)	1005
EcoT38I	GRGCYC	4 cut(s)	114, 133, 512, 1605
ErhI	CCWWGG	4 cut(s)	35, 204, 591, 622
FalI	AAGNNNNNCTT	4 cut(s)	945, 977, 1228, 1260
FaqI	GGGAC	4 cut(s)	362, 857, 1262, 1335
FauNDI	CATATG	3 cut(s)	541, 978, 1597
FbaI	TGATCA	3 cut(s)	1429, 1632, 2196
Fnu4HI	GCNGC	5 cut(s)	41, 775, 1884, 2112, 2208
FokI	GGATG	4 cut(s)	735, 772, 1449, 2058
FriOI	GRGCYC	4 cut(s)	114, 133, 512, 1605
Fsp4HI	GCNGC	5 cut(s)	41, 775, 1884, 2112, 2208
FspAI	RTGCGCAY	1 cut(s)	191
FspBI	CTAG	3 cut(s)	1523, 1911, 2115
FspI	TGCGCA	1 cut(s)	191
GlaI	GCGC	1 cut(s)	191
GluI	GCNGC	5 cut(s)	41, 775, 1884, 2112, 2208
HaeIII	GGCC	9 cut(s)	343, 381, 510, 825, 1009, 1414, 1441, 2051, 2076
HapII	CCGG	4 cut(s)	377, 1368, 1534, 2215
HhaI	GCGC	1 cut(s)	192
Hin6I	GCGC	1 cut(s)	190
HinP1I	GCGC	1 cut(s)	190
HindIII	AAGCTT	1 cut(s)	1923
HinfI	GANTC	5 cut(s)	31, 613, 1179, 1340, 1715
HpaII	CCGG	4 cut(s)	377, 1368, 1534, 2215
HphI	GGTGA	5 cut(s)	575, 1382, 1526, 1667, 2030
Hpy166II	GTNNAC	4 cut(s)	47, 1517, 2002, 2004
Hpy8I	GTNNAC	4 cut(s)	47, 1517, 2002, 2004
HpyAV	CCTTC	5 cut(s)	436, 560, 750, 1457, 1884
HpyCH4III	ACNGT	9 cut(s)	146, 277, 841, 1583, 1619, 1766, 2100, 2108, 2192
HpyCH4IV	ACGT	2 cut(s)	2031, 2201
HpyF10VI	GCNNNNNNNGC	3 cut(s)	416, 1277, 2057
HpyF3I	CTNAG	7 cut(s)	267, 714, 1313, 1447, 1693, 2082, 2271
HpySE526I	ACGT	2 cut(s)	2031, 2201
HspAI	GCGC	1 cut(s)	190
KpnI	GGTACC	1 cut(s)	102
Ksp22I	TGATCA	3 cut(s)	1429, 1632, 2196
Kzo9I	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
LguI	GCTCTTC	2 cut(s)	102, 1041
LmnI	GCTCC	5 cut(s)	117, 179, 587, 677, 744
Lsp1109I	GCAGC	3 cut(s)	27, 1870, 2194
LweI	GCATC	3 cut(s)	1516, 1534, 1814
MaeI	CTAG	3 cut(s)	1523, 1911, 2115
MaeII	ACGT	2 cut(s)	2031, 2201
MaeIII	GTNAC	7 cut(s)	262, 277, 305, 544, 767, 1164, 1655
MalI	GATC	9 cut(s)	87, 122, 449, 1143, 1374, 1431, 1612, 1634, 2198
MboI	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
MfeI	CAATTG	1 cut(s)	1047
MflI	RGATCY	3 cut(s)	447, 1141, 1610
MhlI	GDGCHC	5 cut(s)	114, 133, 512, 1056, 1605
MlsI	TGGCCA	1 cut(s)	2076
MluNI	TGGCCA	1 cut(s)	2076
MlyI	GAGTC	1 cut(s)	40
MmeI	TCCRAC	1 cut(s)	1605
Mox20I	TGGCCA	1 cut(s)	2076
Mph1103I	ATGCAT	1 cut(s)	1005
MroXI	GAANNNNTTC	1 cut(s)	2222
MscI	TGGCCA	1 cut(s)	2076
MseI	TTAA	7 cut(s)	162, 290, 551, 867, 998, 1115, 1722
MslI	CAYNNNNRTG	3 cut(s)	627, 685, 1217
Msp20I	TGGCCA	1 cut(s)	2076
MspA1I	CMGCKG	2 cut(s)	1460, 2253
MspI	CCGG	4 cut(s)	377, 1368, 1534, 2215
MunI	CAATTG	1 cut(s)	1047
Mva1269I	GAATGC	4 cut(s)	192, 421, 1691, 1914
MvaI	CCWGG	7 cut(s)	236, 345, 383, 753, 1011, 1241, 2078
MvnI	CGCG	1 cut(s)	135
MwoI	GCNNNNNNNGC	3 cut(s)	416, 1277, 2057
NciI	CCSGG	3 cut(s)	377, 378, 1368
NcoI	CCATGG	3 cut(s)	35, 591, 622
NdeI	CATATG	3 cut(s)	541, 978, 1597
NdeII	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
NlaIV	GGNNCC	3 cut(s)	100, 510, 589
NmuCI	GTSAC	4 cut(s)	262, 277, 767, 1655
NruI	TCGCGA	1 cut(s)	135
NsbI	TGCGCA	1 cut(s)	191
NsiI	ATGCAT	1 cut(s)	1005
PaeR7I	CTCGAG	1 cut(s)	116
PagI	TCATGA	1 cut(s)	880
PciSI	GCTCTTC	2 cut(s)	102, 1041
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PctI	GAATGC	4 cut(s)	192, 421, 1691, 1914
PdmI	GAANNNNTTC	1 cut(s)	2222
PfeI	GAWTC	4 cut(s)	613, 1179, 1340, 1715
PfoI	TCCNGGA	1 cut(s)	751
PkrI	GCNGC	5 cut(s)	42, 776, 1885, 2113, 2209
PleI	GAGTC	1 cut(s)	39
PmaCI	CACGTG	1 cut(s)	2032
PmlI	CACGTG	1 cut(s)	2032
PpsI	GAGTC	1 cut(s)	39
Ppu21I	YACGTR	2 cut(s)	2032, 2202
PpuMI	RGGWCCY	1 cut(s)	359
Psp124BI	GAGCTC	3 cut(s)	114, 133, 1605
Psp5II	RGGWCCY	1 cut(s)	359
Psp6I	CCWGG	7 cut(s)	234, 343, 381, 751, 1009, 1239, 2076
PspCI	CACGTG	1 cut(s)	2032
PspGI	CCWGG	7 cut(s)	234, 343, 381, 751, 1009, 1239, 2076
PspN4I	GGNNCC	3 cut(s)	100, 510, 589
PspOMI	GGGCCC	1 cut(s)	508
PspPI	GGNCC	9 cut(s)	359, 379, 508, 509, 619, 824, 1097, 1440, 2049
PspPPI	RGGWCCY	1 cut(s)	359
PstNI	CAGNNNCTG	2 cut(s)	221, 2190
PsuI	RGATCY	3 cut(s)	447, 1141, 1610
PvuII	CAGCTG	1 cut(s)	1460
RruI	TCGCGA	1 cut(s)	135
RsaI	GTAC	8 cut(s)	46, 100, 517, 672, 932, 951, 1855, 2003
RsaNI	GTAC	8 cut(s)	45, 99, 516, 671, 931, 950, 1854, 2002
RseI	CAYNNNNRTG	3 cut(s)	627, 685, 1217
SacI	GAGCTC	3 cut(s)	114, 133, 1605
SapI	GCTCTTC	2 cut(s)	102, 1041
SaqAI	TTAA	7 cut(s)	162, 290, 551, 867, 998, 1115, 1722
SatI	GCNGC	5 cut(s)	41, 775, 1884, 2112, 2208
Sau3AI	GATC	9 cut(s)	85, 120, 447, 1141, 1372, 1429, 1610, 1632, 2196
Sau96I	GGNCC	9 cut(s)	359, 379, 508, 509, 619, 824, 1097, 1440, 2049
ScaI	AGTACT	2 cut(s)	517, 672
SchI	GAGTC	1 cut(s)	40
SduI	GDGCHC	5 cut(s)	114, 133, 512, 1056, 1605
SfaNI	GCATC	3 cut(s)	1516, 1534, 1814
SfcI	CTRYAG	1 cut(s)	142
Sfr274I	CTCGAG	1 cut(s)	116
SinI	GGWCC	3 cut(s)	359, 619, 1097
SlaI	CTCGAG	1 cut(s)	116
SmaI	CCCGGG	1 cut(s)	378
SmiMI	CAYNNNNRTG	3 cut(s)	627, 685, 1217
SmlI	CTYRAG	3 cut(s)	116, 2012, 2157
SmoI	CTYRAG	3 cut(s)	116, 2012, 2157
SsiI	CCGC	4 cut(s)	20, 775, 2111, 2251
SspMI	CTAG	3 cut(s)	1523, 1911, 2115
SstI	GAGCTC	3 cut(s)	114, 133, 1605
StyI	CCWWGG	4 cut(s)	35, 204, 591, 622
TaaI	ACNGT	9 cut(s)	146, 277, 841, 1583, 1619, 1766, 2100, 2108, 2192
TaiI	ACGT	2 cut(s)	2034, 2204
TaqI	TCGA	6 cut(s)	84, 117, 123, 454, 1668, 2257
TatI	WGTACW	5 cut(s)	515, 670, 930, 949, 2001
TauI	GCSGC	2 cut(s)	777, 2114
TfiI	GAWTC	4 cut(s)	613, 1179, 1340, 1715
Tru1I	TTAA	7 cut(s)	162, 290, 551, 867, 998, 1115, 1722
Tru9I	TTAA	7 cut(s)	162, 290, 551, 867, 998, 1115, 1722
TscAI	CASTG	7 cut(s)	282, 409, 535, 776, 917, 1048, 1769
TseFI	GTSAC	4 cut(s)	262, 277, 767, 1655
TseI	GCWGC	3 cut(s)	40, 1883, 2207
Tsp45I	GTSAC	4 cut(s)	262, 277, 767, 1655
TspDTI	ATGAA	6 cut(s)	737, 897, 965, 1080, 1229, 1806
TspMI	CCCGGG	1 cut(s)	376
TspRI	CASTG	7 cut(s)	282, 409, 535, 776, 917, 1048, 1769
VpaK11BI	GGWCC	3 cut(s)	359, 619, 1097
XapI	RAATTY	4 cut(s)	1147, 1298, 1494, 2128
XcmI	CCANNNNNNNNNTGG	1 cut(s)	759
XhoI	CTCGAG	1 cut(s)	116
XmaI	CCCGGG	1 cut(s)	376
XmnI	GAANNNNTTC	1 cut(s)	2222
XspI	CTAG	3 cut(s)	1523, 1911, 2115
ZrmI	AGTACT	2 cut(s)	517, 672
Zsp2I	ATGCAT	1 cut(s)	1005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.