Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002493

Physical Location & Seq

Genomic Coordinates Forward (+)

28100853 .. 28108506

Gene Structure 7654 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1130500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGTACTTTTGTAGACCAAAGTGGGAAGGTGGTCAGTATGATGGTGATGAAAAATATCGACTGGATGCACTTCGATTAGCAATGGAGCTTGGAGCTGATTACATTGATGTTGAGCTTCAGGTTGCTCATGAATTCATTGATTCCATATATGGGAAGAAGCCTGAAAAGTTTAAAGTTATTGTCTCTTCTCACAACTATCAAGAAACTCCATCAGTTGAGGCTCTTGGAAATCTTGTTGCAAGAATACAAGCCACTGGAGCTGACATAGTTAAGATTGCAACCAGTGCTATGGATATCACTGATGTAGCACGCATGTTTCACATAACAGTACATTCTCAAGTCCCGACAATAGGACTTGTAATGGGTGAGAGGGGGTTGATTTCACGGATCCTTTGCGCAAAATTTGGTGGTTATCTTACTTTTGGTACGCTTGACTCAGGAATAGTTTCAGCTCCTGGTCAACCAACTATGAAGGATATATTACATCTATACAATTTCAGGCAGATAGGGACTGACACAAAAGTGTTTGGCATTATTGGGAAGCCTGTCAGCCACAGCAAATCACCTATATTGTACAATGAAGCATTCAAGTCAGTAGGTTTCAATGGAGTTTATGTACATTTGTTGATAGATGACATTGTAAATTTTCTCCATACTTACTCTTCAGTGGATTTTGCTGGTTTCAGTGTTACAATTCCGCACAAGGAAGCTGCACTTAAGTGTTGTGATGAAGTTGATCCAGTTGCAAAGGCCATAGGAGCTATTAATTGCATTATACGGAGACCAACTGATGGGAAGTTATTTGGGTTCAATACGGACTATGTAGGGGCAATCTCAGCTATTGAAGACGGACTGAAAGGTTCACATAATAATGGCAGTTTAACCGTTTCACCTTTAGCTGGTAGGCTGTTTGTTGTTATTGGGGCTGGAGGTGCTGGAAAGGCACTTGCTTATGGTGCAAAACAGAAGGGCGCAAGGATTGCAATTGCCAATCGCACTTATGATCGAGCCAGAGAACTTGCAGACACAATTGGAGGAGATGCTTTGTCTCTTGCTGATTTAGATAATTTCCACCCAGAGGATGGGATGATTCTTGCAAATACAACATCTATTGGCATGCAACCAAAAGTTGATGAGACACCCATTTCTAAGCATGCTCTGGGATCTTACTCGTTGGTTTTTGATGCTGTCTACACCCCCAAAATGACCAGACTCTTGAAAGAAGCGAAAGAGGCCGGTGCCACAGTTGTTAGTGGGTTGGAGATGTTCATTGGACAGGCATATGAACAGTTTGAGAGGTTCACTGGGTTGCCTGCCCCAAAGGAACTCTTTCGAAAAGTAATGGACGGTAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004764 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0019632 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055114 GO:0061458 GO:0071704 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

DHquinase_I Shikimate_DH Shikimate_dh_N

Protein Analysis

453

Amino Acids

48.97

Weight (kDa)

6.19

Isoelectric Point (pI)

18.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHquinase_I	PF01487	2 - 164	1e-51	Type I 3-dehydroquinase
Shikimate_dh_N	PF08501	178 - 258	1.1e-25	Shikimate dehydrogenase substrate binding domain
Shikimate_DH	PF01488	299 - 369	8.6e-08	Shikimate / quinate 5-dehydrogenase
Sacchrp_dh_NADP	PF03435	305 - 417	3.3e-06	Saccharopine dehydrogenase NADP binding domain
SDH_C	PF18317	419 - 447	3e-10	Shikimate 5'-dehydrogenase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002493)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06350
fragaria_vesca	FvH4_1g23213 FvH4_1g23213 FvH4_1g23213 FvH4_1g23232 FvH4_1g23232 FvH4_1g23232
malus_domestica	MD00G1130500.v1.1 MD15G1335600.v1.1
prunus_persica	Prupe.6G166100_v2.0.a1 Prupe.6G166100_v2.0.a1 Prupe.6G166100_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1
pyrus_communis	pycom01g04680 pycom01g04700 pycom15g28990
rosa_chinensis	RchiOBHm_Chr2g0117991 RchiOBHm_Chr2g0118061
rosa_laevigata	RLG00000018384 RLG00000018389 RLG00000018397
rosa_multiflora	Rmu_sc0000948.1_g000018 Rmu_sc0000948.1_g000032
rosa_roxburghii	Rroxscaffold_2G00125870 Rroxscaffold_2G00126000 Rroxscaffold_2G00126080 Rroxscaffold_5G00352100
rosa_rugosa	Rorug02G0215100 Rorug02G0215200 Rorug02G0215500.1 Rorug02G0217000
rosa_samantha	Rh2BG283700 Rh2DG280600 Rh2DG298000 Rh2DG298700
rosa_wichuraiana	Rw2G021480 Rw2G021530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	397
AccB1I	GGYRCC	1 cut(s)	1238
AccB7I	CCANNNNNTGG	2 cut(s)	791, 1082
AccI	GTMKAC	2 cut(s)	13, 1191
AciI	CCGC	1 cut(s)	698
AclWI	GGATC	4 cut(s)	382, 395, 731, 1171
AcsI	RAATTY	3 cut(s)	131, 401, 643
AcuI	CTGAAG	2 cut(s)	101, 648
AfaI	GTAC	5 cut(s)	5, 330, 427, 575, 618
AfiI	CCNNNNNNNGG	6 cut(s)	150, 350, 791, 899, 1078, 1082
AflII	CTTAAG	1 cut(s)	716
AgsI	TTSAA	5 cut(s)	589, 604, 811, 845, 1219
AjnI	CCWGG	1 cut(s)	454
AleI	CACNNNNGTG	2 cut(s)	521, 718
Alw26I	GTCTC	4 cut(s)	187, 775, 1053, 1130
AlwI	GGATC	4 cut(s)	382, 395, 731, 1171
AlwNI	CAGNNNCTG	1 cut(s)	455
AoxI	GGCC	2 cut(s)	750, 1233
ApeKI	GCWGC	1 cut(s)	710
ApoI	RAATTY	3 cut(s)	131, 401, 643
AseI	ATTAAT	1 cut(s)	765
Asp700I	GAANNNNTTC	2 cut(s)	445, 1329
AspLEI	GCGC	2 cut(s)	398, 974
AsuHPI	GGTGA	4 cut(s)	56, 377, 555, 882
AsuII	TTCGAA	1 cut(s)	1333
BamHI	GGATCC	1 cut(s)	387
BanI	GGYRCC	1 cut(s)	1238
BbsI	GAAGAC	1 cut(s)	852
BbvI	GCAGC	1 cut(s)	697
BccI	CCATC	4 cut(s)	35, 217, 785, 1076
BciT130I	CCWGG	1 cut(s)	456
BcoDI	GTCTC	4 cut(s)	187, 775, 1053, 1130
BfrI	CTTAAG	1 cut(s)	716
BisI	GCNGC	1 cut(s)	711
BlsI	GCNGC	1 cut(s)	712
Bme1390I	CCNGG	1 cut(s)	456
BmiI	GGNNCC	2 cut(s)	389, 1240
BmrFI	CCNGG	1 cut(s)	456
BmrI	ACTGGG	1 cut(s)	1314
BmsI	GCATC	3 cut(s)	55, 1030, 1174
BmuI	ACTGGG	1 cut(s)	1314
BpiI	GAAGAC	1 cut(s)	852
BpmI	CTGGAG	2 cut(s)	276, 948
Bpu14I	TTCGAA	1 cut(s)	1333
BpuEI	CTTGAG	1 cut(s)	321
BsaI	GGTCTC	1 cut(s)	775
BsaXI	ACNNNNNCTCC	2 cut(s)	600, 630
Bsc4I	CCNNNNNNNGG	6 cut(s)	150, 350, 791, 899, 1078, 1082
Bse118I	RCCGGY	1 cut(s)	1235
Bse1I	ACTGG	5 cut(s)	66, 259, 282, 740, 1309
Bse3DI	GCAATG	1 cut(s)	87
BseBI	CCWGG	1 cut(s)	456
BseGI	GGATG	3 cut(s)	70, 1087, 1092
BseLI	CCNNNNNNNGG	6 cut(s)	150, 350, 791, 899, 1078, 1082
BseMI	GCAATG	1 cut(s)	87
BseMII	CTCAG	2 cut(s)	450, 849
BseNI	ACTGG	5 cut(s)	66, 259, 282, 740, 1309
BseRI	GAGGAG	1 cut(s)	1050
BseXI	GCAGC	1 cut(s)	697
BsgI	GTGCAG	1 cut(s)	696
BshFI	GGCC	2 cut(s)	752, 1235
BshNI	GGYRCC	1 cut(s)	1238
BsiSI	CCGG	1 cut(s)	1236
BslFI	GGGAC	2 cut(s)	326, 523
BslI	CCNNNNNNNGG	6 cut(s)	150, 350, 791, 899, 1078, 1082
BsmAI	GTCTC	4 cut(s)	187, 775, 1053, 1130
BsmFI	GGGAC	2 cut(s)	326, 523
BsmI	GAATGC	1 cut(s)	584
BsnI	GGCC	2 cut(s)	752, 1235
Bso31I	GGTCTC	1 cut(s)	775
Bsp119I	TTCGAA	1 cut(s)	1333
Bsp1407I	TGTACA	2 cut(s)	573, 616
Bsp143I	GATC	4 cut(s)	387, 736, 1003, 1163
BspACI	CCGC	1 cut(s)	698
BspANI	GGCC	2 cut(s)	752, 1235
BspCNI	CTCAG	2 cut(s)	449, 848
BspHI	TCATGA	1 cut(s)	127
BspLI	GGNNCC	2 cut(s)	389, 1240
BspPI	GGATC	4 cut(s)	382, 395, 731, 1171
BspT104I	TTCGAA	1 cut(s)	1333
BspT107I	GGYRCC	1 cut(s)	1238
BspTI	CTTAAG	1 cut(s)	716
BspTNI	GGTCTC	1 cut(s)	775
BsrDI	GCAATG	1 cut(s)	87
BsrFI	RCCGGY	1 cut(s)	1235
BsrGI	TGTACA	2 cut(s)	573, 616
BsrI	ACTGG	5 cut(s)	66, 259, 282, 740, 1309
BssAI	RCCGGY	1 cut(s)	1235
BssMI	GATC	4 cut(s)	387, 736, 1003, 1163
Bst2UI	CCWGG	1 cut(s)	456
Bst4CI	ACNGT	5 cut(s)	328, 886, 1246, 1290, 1349
Bst6I	CTCTTC	2 cut(s)	190, 667
BstAFI	CTTAAG	1 cut(s)	716
BstAPI	GCANNNNNTGC	2 cut(s)	284, 980
BstAUI	TGTACA	2 cut(s)	573, 616
BstBI	TTCGAA	1 cut(s)	1333
BstC8I	GCNNGC	4 cut(s)	310, 1118, 1155, 1314
BstDEI	CTNAG	3 cut(s)	436, 835, 1149
BstF5I	GGATG	3 cut(s)	70, 1087, 1092
BstHHI	GCGC	2 cut(s)	398, 974
BstKTI	GATC	4 cut(s)	390, 739, 1006, 1166
BstMAI	GTCTC	4 cut(s)	187, 775, 1053, 1130
BstMBI	GATC	4 cut(s)	387, 736, 1003, 1163
BstMWI	GCNNNNNNNGC	9 cut(s)	257, 284, 758, 836, 932, 941, 956, 980, 1232
BstNI	CCWGG	1 cut(s)	456
BstNSI	RCATGY	3 cut(s)	316, 1120, 1157
BstSCI	CCNGG	1 cut(s)	454
BstV1I	GCAGC	1 cut(s)	697
BstV2I	GAAGAC	1 cut(s)	852
BstX2I	RGATCY	2 cut(s)	387, 1163
BstXI	CCANNNNNNTGG	1 cut(s)	289
BstYI	RGATCY	2 cut(s)	387, 1163
BsuRI	GGCC	2 cut(s)	752, 1235
BtsCI	GGATG	3 cut(s)	70, 1087, 1092
BtsIMutI	CAGTG	6 cut(s)	252, 289, 297, 672, 691, 1302
Cac8I	GCNNGC	4 cut(s)	310, 1118, 1155, 1314
CaiI	CAGNNNCTG	1 cut(s)	455
CciI	TCATGA	1 cut(s)	127
CfoI	GCGC	2 cut(s)	398, 974
Cfr10I	RCCGGY	1 cut(s)	1235
Csp6I	GTAC	5 cut(s)	4, 329, 426, 574, 617
CviAII	CATG	4 cut(s)	128, 313, 1117, 1154
CviQI	GTAC	5 cut(s)	4, 329, 426, 574, 617
DdeI	CTNAG	3 cut(s)	436, 835, 1149
DpnI	GATC	4 cut(s)	389, 738, 1005, 1165
DpnII	GATC	4 cut(s)	387, 736, 1003, 1163
DraI	TTTAAA	1 cut(s)	172
Eam1104I	CTCTTC	2 cut(s)	190, 667
EarI	CTCTTC	2 cut(s)	190, 667
Eco31I	GGTCTC	1 cut(s)	775
Eco32I	GATATC	1 cut(s)	295
Eco57I	CTGAAG	2 cut(s)	101, 648
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	454
EcoRV	GATATC	1 cut(s)	295
FaeI	CATG	4 cut(s)	131, 316, 1120, 1157
FalI	AAGNNNNNCTT	4 cut(s)	699, 731, 1313, 1345
FaqI	GGGAC	2 cut(s)	326, 523
FatI	CATG	4 cut(s)	127, 312, 1116, 1153
FauNDI	CATATG	1 cut(s)	1282
FblI	GTMKAC	2 cut(s)	13, 1191
Fnu4HI	GCNGC	1 cut(s)	711
FokI	GGATG	3 cut(s)	77, 1094, 1099
Fsp4HI	GCNGC	1 cut(s)	711
FspI	TGCGCA	1 cut(s)	397
GlaI	GCGC	2 cut(s)	397, 973
GluI	GCNGC	1 cut(s)	711
GsuI	CTGGAG	2 cut(s)	276, 948
HaeIII	GGCC	2 cut(s)	752, 1235
HapII	CCGG	1 cut(s)	1236
HhaI	GCGC	2 cut(s)	398, 974
Hin1II	CATG	4 cut(s)	131, 316, 1120, 1157
Hin6I	GCGC	2 cut(s)	396, 972
HinP1I	GCGC	2 cut(s)	396, 972
HincII	GTYRAC	1 cut(s)	461
HindII	GTYRAC	1 cut(s)	461
HinfI	GANTC	4 cut(s)	140, 434, 1090, 1212
HpaII	CCGG	1 cut(s)	1236
HphI	GGTGA	4 cut(s)	56, 377, 555, 882
Hpy166II	GTNNAC	5 cut(s)	14, 461, 863, 1192, 1302
Hpy188III	TCNNGA	5 cut(s)	128, 200, 343, 438, 1216
Hpy8I	GTNNAC	5 cut(s)	14, 461, 863, 1192, 1302
HpyAV	CCTTC	3 cut(s)	20, 466, 961
HpyCH4III	ACNGT	5 cut(s)	328, 886, 1246, 1290, 1349
HpyF10VI	GCNNNNNNNGC	9 cut(s)	257, 284, 758, 836, 932, 941, 956, 980, 1232
HpyF3I	CTNAG	3 cut(s)	436, 835, 1149
Hsp92II	CATG	4 cut(s)	131, 316, 1120, 1157
HspAI	GCGC	2 cut(s)	396, 972
Kzo9I	GATC	4 cut(s)	387, 736, 1003, 1163
LmnI	GCTCC	5 cut(s)	85, 92, 257, 457, 758
Lsp1109I	GCAGC	1 cut(s)	697
LweI	GCATC	3 cut(s)	55, 1030, 1174
MaeIII	GTNAC	1 cut(s)	688
MalI	GATC	4 cut(s)	389, 738, 1005, 1165
MboI	GATC	4 cut(s)	387, 736, 1003, 1163
MboII	GAAGA	4 cut(s)	166, 177, 654, 857
MfeI	CAATTG	2 cut(s)	984, 1029
MflI	RGATCY	2 cut(s)	387, 1163
MluCI	AATT	9 cut(s)	131, 401, 493, 643, 693, 766, 984, 1029, 1066
MlyI	GAGTC	2 cut(s)	428, 1206
MmeI	TCCRAC	1 cut(s)	1239
MnlI	CCTC	7 cut(s)	211, 363, 923, 1028, 1072, 1225, 1290
MroXI	GAANNNNTTC	2 cut(s)	445, 1329
MseI	TTAA	5 cut(s)	171, 270, 717, 765, 881
MslI	CAYNNNNRTG	3 cut(s)	521, 718, 870
MspCI	CTTAAG	1 cut(s)	716
MspI	CCGG	1 cut(s)	1236
MspR9I	CCNGG	1 cut(s)	456
MunI	CAATTG	2 cut(s)	984, 1029
Mva1269I	GAATGC	1 cut(s)	584
MvaI	CCWGG	1 cut(s)	456
MwoI	GCNNNNNNNGC	9 cut(s)	257, 284, 758, 836, 932, 941, 956, 980, 1232
NdeI	CATATG	1 cut(s)	1282
NdeII	GATC	4 cut(s)	387, 736, 1003, 1163
NlaIII	CATG	4 cut(s)	131, 316, 1120, 1157
NlaIV	GGNNCC	2 cut(s)	389, 1240
NsbI	TGCGCA	1 cut(s)	397
NspI	RCATGY	3 cut(s)	316, 1120, 1157
NspV	TTCGAA	1 cut(s)	1333
OliI	CACNNNNGTG	2 cut(s)	521, 718
PaeI	GCATGC	2 cut(s)	1120, 1157
PagI	TCATGA	1 cut(s)	127
PctI	GAATGC	1 cut(s)	584
PdmI	GAANNNNTTC	2 cut(s)	445, 1329
PfeI	GAWTC	2 cut(s)	140, 1090
PflMI	CCANNNNNTGG	2 cut(s)	791, 1082
PkrI	GCNGC	1 cut(s)	712
PleI	GAGTC	2 cut(s)	428, 1206
PpsI	GAGTC	2 cut(s)	428, 1206
PshBI	ATTAAT	1 cut(s)	765
Psp6I	CCWGG	1 cut(s)	454
PspGI	CCWGG	1 cut(s)	454
PspN4I	GGNNCC	2 cut(s)	389, 1240
PstNI	CAGNNNCTG	1 cut(s)	455
PsuI	RGATCY	2 cut(s)	387, 1163
RsaI	GTAC	5 cut(s)	5, 330, 427, 575, 618
RsaNI	GTAC	5 cut(s)	4, 329, 426, 574, 617
RseI	CAYNNNNRTG	3 cut(s)	521, 718, 870
SaqAI	TTAA	5 cut(s)	171, 270, 717, 765, 881
SatI	GCNGC	1 cut(s)	711
Sau3AI	GATC	4 cut(s)	387, 736, 1003, 1163
SchI	GAGTC	2 cut(s)	428, 1206
ScrFI	CCNGG	1 cut(s)	456
SfaNI	GCATC	3 cut(s)	55, 1030, 1174
SfuI	TTCGAA	1 cut(s)	1333
SmiMI	CAYNNNNRTG	3 cut(s)	521, 718, 870
SmlI	CTYRAG	2 cut(s)	336, 716
SmoI	CTYRAG	2 cut(s)	336, 716
SphI	GCATGC	2 cut(s)	1120, 1157
Sse9I	AATT	9 cut(s)	131, 401, 493, 643, 693, 766, 984, 1029, 1066
SsiI	CCGC	1 cut(s)	698
StyD4I	CCNGG	1 cut(s)	454
TaaI	ACNGT	5 cut(s)	328, 886, 1246, 1290, 1349
TaqI	TCGA	4 cut(s)	58, 73, 1006, 1333
TasI	AATT	9 cut(s)	131, 401, 493, 643, 693, 766, 984, 1029, 1066
TatI	WGTACW	4 cut(s)	3, 328, 573, 616
TfiI	GAWTC	2 cut(s)	140, 1090
Tru1I	TTAA	5 cut(s)	171, 270, 717, 765, 881
Tru9I	TTAA	5 cut(s)	171, 270, 717, 765, 881
TscAI	CASTG	6 cut(s)	259, 289, 304, 672, 691, 1309
TseI	GCWGC	1 cut(s)	710
TspDTI	ATGAA	8 cut(s)	63, 124, 144, 485, 594, 744, 1258, 1299
TspGWI	ACGGA	4 cut(s)	400, 793, 830, 864
TspRI	CASTG	6 cut(s)	259, 289, 304, 672, 691, 1309
Van91I	CCANNNNNTGG	2 cut(s)	791, 1082
Vha464I	CTTAAG	1 cut(s)	716
VspI	ATTAAT	1 cut(s)	765
XapI	RAATTY	3 cut(s)	131, 401, 643
XceI	RCATGY	3 cut(s)	316, 1120, 1157
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1079
XmiI	GTMKAC	2 cut(s)	13, 1191
XmnI	GAANNNNTTC	2 cut(s)	445, 1329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.