Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002493

Physical Location & Seq

Genomic Coordinates Forward (+)

29817639 .. 29823591

Gene Structure 5953 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G021480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGACTCACCAAACCTTGCGGTTGCTTCAGCACAAGTGGGAGGCGGAGGAATGAGGAAGAGTTCAACTCTAATTTGTGCTCCGATCATGGCCGAATCGGTGGCCATGATGGTGGTTGAGATTGGCAGGGCAAAGGCCGCTGGTGCTGACCTTGTGGAGATTCGATTGGACCATTTGAAGGTCTTCAATTGTAATCAAGATGTCAAAACCCTAGTTGATCAATCCGCTTTGCCCACTCTTTTCACTTATAGACCAAAGTGGGAAGGTGGTCAGTATGATGGCGATGAAAAGAGCAGACTAGATGTGCTTCGACTAGCTATGGAGTTGGGGGCAGATTACATTGATGTTGAGCTTCAGGTTGCTCAAGAATTTATTGACTCCATACAAGACAAGAAGCCTGAAAAGCTTAAAGTTATTGTTTCTTCTCACAACTATCAAGAAACTCCATCTGTTGAGGCTCTTGGCAATCTTGTTGCAAAAATACAAGCCACTGGAGCTGATATAGTGAAGATTGCGACCTTTGCCTTGGATATCACTGATGTCGCTCGCATTTTTCAAATAACCGTGCATTCTCAAGTCCCAATAATTGGACTTGTAATGGGTGAAAGGGGTTTGATTTCACGGATACTTTGCGCCAAATTTGGTGGTTATCTTACCTTTGGCACAATTGATTCAGGAGCAGTTTCAGCTCCTGGTCAACCTACTATCAAGGATATCTTGCATCTATACAATGTCAGACAGATAGGGCCTGACACAAAAGTGTTCGGTATTATTGGAAAGCCTGTCAGCCACAGCAAATCGCCTATTTTGCATAATGAAGGATTCAAGTCGGTAGGTTTCAATGGAGTTTATGTACATTTGTTGGTGGATGACATTGCCAAATTTCTCAAGACATACTCATCTACGGATTTTGCTGGTTTCAGTGTTACAATTCCTCACAAGGAAGCTGCACTTAAGTGCTGTGACGAGGTTGATCCAGTTGCAAAGTCAATAGGAGCTATTAATTGCATTATAAGGAGACCAACTGATGGAAAGTTATATGGATTAAATACGGACTATGTCGGGGCTATCTCTGCCATAGAGGATGGACTGCGAGGTTCACTCAACAGTTCCCTTGTAACTGGTTCCCCTTTAGCTGGTAGGCTATTTGTTGTCATAGGTGCCGGAGGTGCTGGCAAGGCCCTTGCTTATGGCGCAAAACAGAAGGGAGCAAGGATTGTGATTGCCAACCGCACCTATGATCGAGCCAGAGAAATTGCAGACACAATTGGAGGACAGGCTTTGTCTATTTCTGATCTAGATAAATTCTACCCAGAGGATGGCATGATTCTTGCAAACACAACATCTATTGGAATGCAGCCCAAAGTTGATGAGACACCCATTTCTAAGCATGCTCTGAAATCTTACGCTTTGGTGTTTGATGCCGTTTACACCCCCAAAATAACCAGACTCTTGAAGGAAGCACAAGAGTCTGGTGCCATAGTTGTTAGTGGGTTGGAGATGTTCATTGGACAGGCATATGAACAGTTCGAGAGGTTCACCGGATTGCCTGCACCAAAGGAACTCTTCAGGAAAGTTGTGGAGAGTACCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004764 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0019632 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055114 GO:0061458 GO:0071704 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

DHquinase_I Shikimate_DH Shikimate_dh_N

Protein Analysis

531

Amino Acids

57.03

Weight (kDa)

6.35

Isoelectric Point (pI)

24.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHquinase_I	PF01487	26 - 243	1.3e-68	Type I 3-dehydroquinase
Shikimate_dh_N	PF08501	257 - 337	5.5e-27	Shikimate dehydrogenase substrate binding domain
Shikimate_DH	PF01488	379 - 449	4.1e-08	Shikimate / quinate 5-dehydrogenase
Sacchrp_dh_NADP	PF03435	384 - 496	4.4e-07	Saccharopine dehydrogenase NADP binding domain
SDH_C	PF18317	498 - 528	8.6e-11	Shikimate 5'-dehydrogenase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002493)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06350
fragaria_vesca	FvH4_1g23213 FvH4_1g23213 FvH4_1g23213 FvH4_1g23232 FvH4_1g23232 FvH4_1g23232
malus_domestica	MD00G1130500.v1.1 MD15G1335600.v1.1
prunus_persica	Prupe.6G166100_v2.0.a1 Prupe.6G166100_v2.0.a1 Prupe.6G166100_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1
pyrus_communis	pycom01g04680 pycom01g04700 pycom15g28990
rosa_chinensis	RchiOBHm_Chr2g0117991 RchiOBHm_Chr2g0118061
rosa_laevigata	RLG00000018384 RLG00000018389 RLG00000018397
rosa_multiflora	Rmu_sc0000948.1_g000018 Rmu_sc0000948.1_g000032
rosa_roxburghii	Rroxscaffold_2G00125870 Rroxscaffold_2G00126000 Rroxscaffold_2G00126080 Rroxscaffold_5G00352100
rosa_rugosa	Rorug02G0215100 Rorug02G0215200 Rorug02G0215500.1 Rorug02G0217000
rosa_samantha	Rh2BG283700 Rh2DG280600 Rh2DG298000 Rh2DG298700
rosa_wichuraiana	Rw2G021480 Rw2G021530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1013
AccB1I	GGYRCC	2 cut(s)	1160, 1475
AccB7I	CCANNNNNTGG	3 cut(s)	587, 1028, 1319
AciI	CCGC	5 cut(s)	20, 45, 138, 225, 1231
AclWI	GGATC	1 cut(s)	968
AcoI	YGGCCR	2 cut(s)	90, 102
AcsI	RAATTY	4 cut(s)	368, 638, 881, 1304
AcuI	CTGAAG	3 cut(s)	12, 338, 1552
AfaI	GTAC	2 cut(s)	855, 1588
AfiI	CCNNNNNNNGG	5 cut(s)	178, 587, 1028, 1136, 1319
AflII	CTTAAG	1 cut(s)	953
AgsI	TTSAA	7 cut(s)	66, 178, 187, 557, 826, 841, 1456
AjnI	CCWGG	1 cut(s)	691
AleI	CACNNNNGTG	2 cut(s)	758, 955
AluBI	AGCT	8 cut(s)	317, 352, 406, 497, 689, 947, 998, 1136
AluI	AGCT	8 cut(s)	317, 352, 406, 497, 689, 947, 998, 1136
Alw21I	GWGCWC	1 cut(s)	82
Alw26I	GTCTC	2 cut(s)	1012, 1367
AlwI	GGATC	1 cut(s)	968
AlwNI	CAGNNNCTG	1 cut(s)	692
AoxI	GGCC	5 cut(s)	90, 102, 135, 746, 1179
ApeKI	GCWGC	2 cut(s)	947, 1357
ApoI	RAATTY	4 cut(s)	368, 638, 881, 1304
AseI	ATTAAT	1 cut(s)	1002
Asp700I	GAANNNNTTC	1 cut(s)	182
AspLEI	GCGC	2 cut(s)	635, 1196
AspS9I	GGNCC	3 cut(s)	169, 746, 1180
AsuHPI	GGTGA	2 cut(s)	614, 1531
AvaII	GGWCC	1 cut(s)	169
BalI	TGGCCA	1 cut(s)	104
BanI	GGYRCC	2 cut(s)	1160, 1475
BbsI	GAAGAC	1 cut(s)	175
Bbv12I	GWGCWC	1 cut(s)	82
BbvI	GCAGC	2 cut(s)	934, 1369
BccI	CCATC	6 cut(s)	103, 272, 454, 1022, 1079, 1313
BceAI	ACGGC	1 cut(s)	1409
BciT130I	CCWGG	1 cut(s)	693
BciVI	GTATCC	1 cut(s)	618
BclI	TGATCA	1 cut(s)	217
BcoDI	GTCTC	2 cut(s)	1012, 1367
BfaI	CTAG	4 cut(s)	212, 299, 314, 1298
BfrI	CTTAAG	1 cut(s)	953
BfuI	GTATCC	1 cut(s)	618
BisI	GCNGC	3 cut(s)	138, 948, 1358
BlsI	GCNGC	3 cut(s)	139, 949, 1359
Bme1390I	CCNGG	1 cut(s)	693
Bme18I	GGWCC	1 cut(s)	169
BmgT120I	GGNCC	3 cut(s)	169, 746, 1180
BmiI	GGNNCC	3 cut(s)	1126, 1162, 1477
BmrFI	CCNGG	1 cut(s)	693
BmsI	GCATC	2 cut(s)	730, 1411
BpiI	GAAGAC	1 cut(s)	175
BplI	GAGNNNNNCTC	5 cut(s)	52, 84, 1086, 1118, 1574
BpmI	CTGGAG	1 cut(s)	513
BpuEI	CTTGAG	3 cut(s)	348, 558, 872
BsaI	GGTCTC	1 cut(s)	1012
BsaJI	CCNNGG	1 cut(s)	525
BsaWI	WCCGGW	1 cut(s)	1541
BsaXI	ACNNNNNCTCC	2 cut(s)	837, 867
Bsc4I	CCNNNNNNNGG	5 cut(s)	178, 587, 1028, 1136, 1319
Bse1I	ACTGG	3 cut(s)	496, 977, 1126
Bse3DI	GCAATG	1 cut(s)	873
BseBI	CCWGG	1 cut(s)	693
BseDI	CCNNGG	1 cut(s)	525
BseGI	GGATG	3 cut(s)	874, 1090, 1324
BseLI	CCNNNNNNNGG	5 cut(s)	178, 587, 1028, 1136, 1319
BseMI	GCAATG	1 cut(s)	873
BseNI	ACTGG	3 cut(s)	496, 977, 1126
BseXI	GCAGC	2 cut(s)	934, 1369
BsgI	GTGCAG	2 cut(s)	933, 1536
BshFI	GGCC	5 cut(s)	92, 104, 137, 748, 1181
BshNI	GGYRCC	2 cut(s)	1160, 1475
BsiHKAI	GWGCWC	1 cut(s)	82
BsiSI	CCGG	2 cut(s)	1164, 1542
BslFI	GGGAC	1 cut(s)	563
BslI	CCNNNNNNNGG	5 cut(s)	178, 587, 1028, 1136, 1319
BsmAI	GTCTC	2 cut(s)	1012, 1367
BsmFI	GGGAC	1 cut(s)	563
BsmI	GAATGC	2 cut(s)	568, 1359
BsnI	GGCC	5 cut(s)	92, 104, 137, 748, 1181
Bso31I	GGTCTC	1 cut(s)	1012
Bsp1286I	GDGCHC	1 cut(s)	82
Bsp1407I	TGTACA	1 cut(s)	853
Bsp143I	GATC	5 cut(s)	84, 217, 973, 1240, 1294
BspACI	CCGC	5 cut(s)	20, 45, 138, 225, 1231
BspANI	GGCC	5 cut(s)	92, 104, 137, 748, 1181
BspLI	GGNNCC	3 cut(s)	1126, 1162, 1477
BspPI	GGATC	1 cut(s)	968
BspT107I	GGYRCC	2 cut(s)	1160, 1475
BspTI	CTTAAG	1 cut(s)	953
BspTNI	GGTCTC	1 cut(s)	1012
BsrDI	GCAATG	1 cut(s)	873
BsrGI	TGTACA	1 cut(s)	853
BsrI	ACTGG	3 cut(s)	496, 977, 1126
BssECI	CCNNGG	1 cut(s)	525
BssMI	GATC	5 cut(s)	84, 217, 973, 1240, 1294
BssT1I	CCWWGG	1 cut(s)	525
Bst2UI	CCWGG	1 cut(s)	693
Bst4CI	ACNGT	3 cut(s)	565, 1109, 1527
Bst6I	CTCTTC	2 cut(s)	53, 1571
BstAFI	CTTAAG	1 cut(s)	953
BstAUI	TGTACA	1 cut(s)	853
BstC8I	GCNNGC	4 cut(s)	547, 1174, 1392, 1551
BstDEI	CTNAG	1 cut(s)	1386
BstF5I	GGATG	3 cut(s)	874, 1090, 1324
BstHHI	GCGC	2 cut(s)	635, 1196
BstKTI	GATC	5 cut(s)	87, 220, 976, 1243, 1297
BstMAI	GTCTC	2 cut(s)	1012, 1367
BstMBI	GATC	5 cut(s)	84, 217, 973, 1240, 1294
BstNI	CCWGG	1 cut(s)	693
BstNSI	RCATGY	1 cut(s)	1394
BstSCI	CCNGG	1 cut(s)	691
BstV1I	GCAGC	2 cut(s)	934, 1369
BstV2I	GAAGAC	1 cut(s)	175
BstXI	CCANNNNNNTGG	1 cut(s)	112
BsuI	GTATCC	1 cut(s)	618
BsuRI	GGCC	5 cut(s)	92, 104, 137, 748, 1181
BtgZI	GCGATG	1 cut(s)	297
BtsCI	GGATG	3 cut(s)	874, 1090, 1324
BtsIMutI	CAGTG	3 cut(s)	489, 534, 928
Cac8I	GCNNGC	4 cut(s)	547, 1174, 1392, 1551
CaiI	CAGNNNCTG	1 cut(s)	692
CfoI	GCGC	2 cut(s)	635, 1196
Cfr13I	GGNCC	3 cut(s)	169, 746, 1180
Csp6I	GTAC	2 cut(s)	854, 1587
CspCI	CAANNNNNGTGG	2 cut(s)	625, 660
CviAII	CATG	4 cut(s)	88, 106, 1324, 1391
CviQI	GTAC	2 cut(s)	854, 1587
DdeI	CTNAG	1 cut(s)	1386
DpnI	GATC	5 cut(s)	86, 219, 975, 1242, 1296
DpnII	GATC	5 cut(s)	84, 217, 973, 1240, 1294
EaeI	YGGCCR	2 cut(s)	90, 102
Eam1104I	CTCTTC	2 cut(s)	53, 1571
EarI	CTCTTC	2 cut(s)	53, 1571
EciI	GGCGGA	1 cut(s)	60
Eco130I	CCWWGG	1 cut(s)	525
Eco31I	GGTCTC	1 cut(s)	1012
Eco32I	GATATC	2 cut(s)	532, 715
Eco47I	GGWCC	1 cut(s)	169
Eco57I	CTGAAG	3 cut(s)	12, 338, 1552
EcoO109I	RGGNCCY	2 cut(s)	746, 1180
EcoRII	CCWGG	1 cut(s)	691
EcoRV	GATATC	2 cut(s)	532, 715
EcoT14I	CCWWGG	1 cut(s)	525
ErhI	CCWWGG	1 cut(s)	525
FaeI	CATG	4 cut(s)	91, 109, 1327, 1394
FalI	AAGNNNNNCTT	6 cut(s)	701, 733, 936, 968, 1550, 1582
FaqI	GGGAC	1 cut(s)	563
FatI	CATG	4 cut(s)	87, 105, 1323, 1390
FauNDI	CATATG	1 cut(s)	1519
FbaI	TGATCA	1 cut(s)	217
Fnu4HI	GCNGC	3 cut(s)	138, 948, 1358
FokI	GGATG	3 cut(s)	881, 1097, 1331
Fsp4HI	GCNGC	3 cut(s)	138, 948, 1358
FspBI	CTAG	4 cut(s)	212, 299, 314, 1298
GlaI	GCGC	2 cut(s)	634, 1195
GluI	GCNGC	3 cut(s)	138, 948, 1358
GsuI	CTGGAG	1 cut(s)	513
HaeIII	GGCC	5 cut(s)	92, 104, 137, 748, 1181
HapII	CCGG	2 cut(s)	1164, 1542
HhaI	GCGC	2 cut(s)	635, 1196
Hin1II	CATG	4 cut(s)	91, 109, 1327, 1394
Hin6I	GCGC	2 cut(s)	633, 1194
HinP1I	GCGC	2 cut(s)	633, 1194
HincII	GTYRAC	1 cut(s)	698
HindII	GTYRAC	1 cut(s)	698
HindIII	AAGCTT	1 cut(s)	404
HinfI	GANTC	9 cut(s)	5, 95, 160, 377, 671, 822, 1327, 1449, 1469
HpaII	CCGG	2 cut(s)	1164, 1542
HphI	GGTGA	2 cut(s)	614, 1531
Hpy166II	GTNNAC	4 cut(s)	698, 1100, 1429, 1539
Hpy188I	TCNGA	4 cut(s)	84, 737, 1294, 1398
Hpy8I	GTNNAC	4 cut(s)	698, 1100, 1429, 1539
HpyAV	CCTTC	5 cut(s)	172, 257, 812, 1198, 1450
HpyCH4III	ACNGT	3 cut(s)	565, 1109, 1527
HpyF3I	CTNAG	1 cut(s)	1386
Hsp92II	CATG	4 cut(s)	91, 109, 1327, 1394
HspAI	GCGC	2 cut(s)	633, 1194
Ksp22I	TGATCA	1 cut(s)	217
Kzo9I	GATC	5 cut(s)	84, 217, 973, 1240, 1294
LmnI	GCTCC	6 cut(s)	85, 494, 677, 694, 995, 1208
Lsp1109I	GCAGC	2 cut(s)	934, 1369
LweI	GCATC	2 cut(s)	730, 1411
MaeI	CTAG	4 cut(s)	212, 299, 314, 1298
MaeIII	GTNAC	3 cut(s)	925, 962, 1117
MalI	GATC	5 cut(s)	86, 219, 975, 1242, 1296
MboI	GATC	5 cut(s)	84, 217, 973, 1240, 1294
MboII	GAAGA	5 cut(s)	70, 175, 414, 520, 1558
MfeI	CAATTG	3 cut(s)	187, 666, 1266
MhlI	GDGCHC	1 cut(s)	82
MlsI	TGGCCA	1 cut(s)	104
MluNI	TGGCCA	1 cut(s)	104
MlyI	GAGTC	3 cut(s)	371, 1443, 1478
MmeI	TCCRAC	1 cut(s)	1476
Mox20I	TGGCCA	1 cut(s)	104
MroXI	GAANNNNTTC	1 cut(s)	182
MscI	TGGCCA	1 cut(s)	104
MseI	TTAA	4 cut(s)	408, 954, 1002, 1046
MslI	CAYNNNNRTG	3 cut(s)	110, 758, 955
Msp20I	TGGCCA	1 cut(s)	104
MspA1I	CMGCKG	1 cut(s)	140
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	2 cut(s)	1164, 1542
MspR9I	CCNGG	1 cut(s)	693
MunI	CAATTG	3 cut(s)	187, 666, 1266
Mva1269I	GAATGC	2 cut(s)	568, 1359
MvaI	CCWGG	1 cut(s)	693
NdeI	CATATG	1 cut(s)	1519
NdeII	GATC	5 cut(s)	84, 217, 973, 1240, 1294
NlaIII	CATG	4 cut(s)	91, 109, 1327, 1394
NlaIV	GGNNCC	3 cut(s)	1126, 1162, 1477
NmuCI	GTSAC	1 cut(s)	962
NspI	RCATGY	1 cut(s)	1394
OliI	CACNNNNGTG	2 cut(s)	758, 955
PaeI	GCATGC	1 cut(s)	1394
PctI	GAATGC	2 cut(s)	568, 1359
PdmI	GAANNNNTTC	1 cut(s)	182
PfeI	GAWTC	5 cut(s)	95, 160, 671, 822, 1327
PflFI	GACNNNGTC	1 cut(s)	1058
PflMI	CCANNNNNTGG	3 cut(s)	587, 1028, 1319
PkrI	GCNGC	3 cut(s)	139, 949, 1359
PleI	GAGTC	3 cut(s)	371, 1443, 1477
PpsI	GAGTC	3 cut(s)	371, 1443, 1477
PshBI	ATTAAT	1 cut(s)	1002
PsiI	TTATAA	1 cut(s)	1013
Psp6I	CCWGG	1 cut(s)	691
PspGI	CCWGG	1 cut(s)	691
PspN4I	GGNNCC	3 cut(s)	1126, 1162, 1477
PspPI	GGNCC	3 cut(s)	169, 746, 1180
PstNI	CAGNNNCTG	1 cut(s)	692
PsyI	GACNNNGTC	1 cut(s)	1058
RsaI	GTAC	2 cut(s)	855, 1588
RsaNI	GTAC	2 cut(s)	854, 1587
RseI	CAYNNNNRTG	3 cut(s)	110, 758, 955
SaqAI	TTAA	4 cut(s)	408, 954, 1002, 1046
SatI	GCNGC	3 cut(s)	138, 948, 1358
Sau3AI	GATC	5 cut(s)	84, 217, 973, 1240, 1294
Sau96I	GGNCC	3 cut(s)	169, 746, 1180
SchI	GAGTC	3 cut(s)	371, 1443, 1478
ScrFI	CCNGG	1 cut(s)	693
SduI	GDGCHC	1 cut(s)	82
SfaNI	GCATC	2 cut(s)	730, 1411
SinI	GGWCC	1 cut(s)	169
SmiMI	CAYNNNNRTG	3 cut(s)	110, 758, 955
SmlI	CTYRAG	4 cut(s)	363, 573, 887, 953
SmoI	CTYRAG	4 cut(s)	363, 573, 887, 953
SphI	GCATGC	1 cut(s)	1394
SsiI	CCGC	5 cut(s)	20, 45, 138, 225, 1231
SspMI	CTAG	4 cut(s)	212, 299, 314, 1298
StyD4I	CCNGG	1 cut(s)	691
StyI	CCWWGG	1 cut(s)	525
TaaI	ACNGT	3 cut(s)	565, 1109, 1527
TaqI	TCGA	4 cut(s)	163, 310, 1243, 1530
TatI	WGTACW	1 cut(s)	853
TauI	GCSGC	1 cut(s)	140
TfiI	GAWTC	5 cut(s)	95, 160, 671, 822, 1327
Tru1I	TTAA	4 cut(s)	408, 954, 1002, 1046
Tru9I	TTAA	4 cut(s)	408, 954, 1002, 1046
TscAI	CASTG	3 cut(s)	496, 541, 928
TseFI	GTSAC	1 cut(s)	962
TseI	GCWGC	2 cut(s)	947, 1357
Tsp45I	GTSAC	1 cut(s)	962
TspDTI	ATGAA	4 cut(s)	300, 831, 1495, 1536
TspGWI	ACGGA	3 cut(s)	637, 920, 1067
TspRI	CASTG	3 cut(s)	496, 541, 928
Tth111I	GACNNNGTC	1 cut(s)	1058
Van91I	CCANNNNNTGG	3 cut(s)	587, 1028, 1319
Vha464I	CTTAAG	1 cut(s)	953
VpaK11BI	GGWCC	1 cut(s)	169
VspI	ATTAAT	1 cut(s)	1002
XapI	RAATTY	4 cut(s)	368, 638, 881, 1304
XbaI	TCTAGA	1 cut(s)	1297
XceI	RCATGY	1 cut(s)	1394
XmnI	GAANNNNTTC	1 cut(s)	182
XspI	CTAG	4 cut(s)	212, 299, 314, 1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.