Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002493

Physical Location & Seq

Genomic Coordinates Reverse (-)

61086823 .. 61092711

Gene Structure 5889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00126000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGATTTTAGTACTACCGGTGTCATTTTGCTCATTATAAACATAATTTGTCCGATGATTTTCCTGCGAAACTCCCAGCAACTCCCATCTGATAGTTTGTACTTTTCTAGACCAAAGTGGCAAGGTGGTCAGTATGATGGTGATGAAAAACATCGACTGGATGCTCTTCGATTAGCGATGGAGCTTGGAGCTGATTACATTGATGTCGAGTTTCAGGTTGCTCATGAATTCATCGATTCCATATATGGAAAGAAGCCTGACAAGTTTAAAGTTATTGTTTCTTCCCACAACTATCAAGATACTCCATCTGTCGAGGATCTCGGAGATCTTGTTGCTAGAATGCAAGCCACTGGAGCTGACATAGTCAGCATCACAACCACTGCCTTGGATATTACTGACGTAGCACGCATTTTTCAGATAACTGTGCATTCTCAAGTCCCAGTAATAGGACTTGCAATGGGTGAGAGGGGCTTGATTTCTAGGATACTCTGCGCAAAATTTGGTGGTTACCTTACTTCTGGTACACTCGATTCAGGAATTGTTTCAGTTCCTAAAGAACCTGCCATCAAGGATATATTACATGTATACAATTTCAGACTGATAAGACCTGATACAAAACTGTTTGGTGTTATTGGAAAGCCTATCAGCCACAGCAAATCTCCTCTTCTCTACAATGAAGGATTCAAGTCAGTAGGTTTCGATGGAGTTTATCTACATCTTTTGGTAGATGACATTGCAAATTTTCTCCGTACTTACTCATCAACTGACTTTTCTGGATTCAGCGTTGGAATTCCTCACAAGGAAGCTGCAGTTAAGTGCTGTGATGAGGTTGATCCAGTTGCCAAGTCAATAGGAGCTGTTAACTGCATTGTAAGGAGGCCAACTGATGGGAAGTTACTTGGCTTAAATACTGACTATTTTGGGTCTATTTCTGCTATCGAGGATGGACTTCGAGGTTCACATGATAGAGGCAATACCACTGGTTCACCTTTAGCTGGTAGGTTGTTTGTTGTCATGGGTGCCGGAGGTGCTGGAAAGGCGCTTGCTTATGGTGCAAAGCAGAAGGGAGCTAGAATTGTTATTGCCAACCGCAAATATGATCGAGCCAGAAAACTTGCAGACGAAGTTGGAGGAGATGCTTTGTCTTTCGCTGAATTAGAAAATTTCCACCCAGAGGACGGCACGGTTCTTGCAAATGCAACATCTGTCGGAATGCAACCGAATATTGATGAGACACCTATTCCTAAGCATGCTCTGAGATCATATTCGCTGGTGTTTGATGCCGTTTACAACCCCAAAATGACTAGACTTTTGAGCGAAGCTGCGGAGTCCGGTGTCACAGTTGTTAGTGGGGTTGAGATGCTCATTGGACAGGCATATGAACAGTTTGAGAGGTTCACCGGATTGCCTGCACCAAAGAAACTGTTTAGGAATGTTATGGAAAATTGCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004764 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0019632 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055114 GO:0061458 GO:0071704 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

DHquinase_I Shikimate_DH Shikimate_dh_N

Protein Analysis

484

Amino Acids

52.66

Weight (kDa)

6.03

Isoelectric Point (pI)

24.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHquinase_I	PF01487	26 - 196	2.8e-45	Type I 3-dehydroquinase
Shikimate_dh_N	PF08501	210 - 290	5.6e-25	Shikimate dehydrogenase substrate binding domain
Shikimate_DH	PF01488	332 - 388	1.7e-06	Shikimate / quinate 5-dehydrogenase
SDH_C	PF18317	451 - 480	1.1e-09	Shikimate 5'-dehydrogenase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002493)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06350
fragaria_vesca	FvH4_1g23213 FvH4_1g23213 FvH4_1g23213 FvH4_1g23232 FvH4_1g23232 FvH4_1g23232
malus_domestica	MD00G1130500.v1.1 MD15G1335600.v1.1
prunus_persica	Prupe.6G166100_v2.0.a1 Prupe.6G166100_v2.0.a1 Prupe.6G166100_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1 Prupe.6G166200_v2.0.a1
pyrus_communis	pycom01g04680 pycom01g04700 pycom15g28990
rosa_chinensis	RchiOBHm_Chr2g0117991 RchiOBHm_Chr2g0118061
rosa_laevigata	RLG00000018384 RLG00000018389 RLG00000018397
rosa_multiflora	Rmu_sc0000948.1_g000018 Rmu_sc0000948.1_g000032
rosa_roxburghii	Rroxscaffold_2G00125870 Rroxscaffold_2G00126000 Rroxscaffold_2G00126080 Rroxscaffold_5G00352100
rosa_rugosa	Rorug02G0215100 Rorug02G0215200 Rorug02G0215500.1 Rorug02G0217000
rosa_samantha	Rh2BG283700 Rh2DG280600 Rh2DG298000 Rh2DG298700
rosa_wichuraiana	Rw2G021480 Rw2G021530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	38
Acc16I	TGCGCA	1 cut(s)	493
Acc36I	ACCTGC	1 cut(s)	568
AccB1I	GGYRCC	1 cut(s)	1019
AccB7I	CCANNNNNTGG	1 cut(s)	887
AccI	GTMKAC	1 cut(s)	585
AciI	CCGC	2 cut(s)	1090, 1325
AclWI	GGATC	2 cut(s)	324, 827
AcsI	RAATTY	5 cut(s)	227, 497, 739, 789, 1162
AfaI	GTAC	4 cut(s)	13, 101, 523, 751
AfiI	CCNNNNNNNGG	5 cut(s)	446, 887, 995, 1174, 1178
AflIII	ACRYGT	1 cut(s)	580
AgeI	ACCGGT	1 cut(s)	17
AgsI	TTSAA	1 cut(s)	685
AluBI	AGCT	8 cut(s)	184, 191, 356, 806, 857, 995, 1070, 1322
AluI	AGCT	8 cut(s)	184, 191, 356, 806, 857, 995, 1070, 1322
Alw26I	GTCTC	1 cut(s)	1226
AlwI	GGATC	2 cut(s)	324, 827
AoxI	GGCC	1 cut(s)	878
ApeKI	GCWGC	2 cut(s)	806, 1322
ApoI	RAATTY	5 cut(s)	227, 497, 739, 789, 1162
ArsI	GACNNNNNNTTYG	2 cut(s)	1294, 1326
AsiGI	ACCGGT	1 cut(s)	17
Asp700I	GAANNNNTTC	1 cut(s)	541
AspLEI	GCGC	2 cut(s)	494, 1042
AsuHPI	GGTGA	4 cut(s)	152, 473, 978, 1390
BanI	GGYRCC	1 cut(s)	1019
BbvI	GCAGC	2 cut(s)	793, 1309
BccI	CCATC	8 cut(s)	94, 131, 172, 313, 572, 695, 881, 938
BceAI	ACGGC	2 cut(s)	1195, 1268
BciVI	GTATCC	1 cut(s)	477
BcoDI	GTCTC	1 cut(s)	1226
BfaI	CTAG	5 cut(s)	108, 336, 480, 1071, 1305
BfmI	CTRYAG	1 cut(s)	807
BfoI	RGCGCY	1 cut(s)	1043
BfuAI	ACCTGC	1 cut(s)	568
BfuI	GTATCC	1 cut(s)	477
BglII	AGATCT	1 cut(s)	325
BisI	GCNGC	2 cut(s)	807, 1323
BlsI	GCNGC	2 cut(s)	808, 1324
BmcAI	AGTACT	1 cut(s)	13
BmiI	GGNNCC	1 cut(s)	1021
BmrI	ACTGGG	1 cut(s)	434
BmsI	GCATC	5 cut(s)	151, 378, 1126, 1270, 1350
BmuI	ACTGGG	1 cut(s)	434
BpmI	CTGGAG	1 cut(s)	372
Bpu10I	CCTNAGC	1 cut(s)	1245
BpuEI	CTTGAG	1 cut(s)	417
Bsa29I	ATCGAT	1 cut(s)	234
BsaJI	CCNNGG	1 cut(s)	384
BsaWI	WCCGGW	3 cut(s)	17, 1331, 1400
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	5 cut(s)	446, 887, 995, 1174, 1178
Bse118I	RCCGGY	1 cut(s)	17
Bse1I	ACTGG	5 cut(s)	162, 355, 440, 836, 985
Bse3DI	GCAATG	2 cut(s)	462, 732
BseCI	ATCGAT	1 cut(s)	234
BseDI	CCNNGG	1 cut(s)	384
BseGI	GGATG	2 cut(s)	166, 949
BseLI	CCNNNNNNNGG	5 cut(s)	446, 887, 995, 1174, 1178
BseMI	GCAATG	2 cut(s)	462, 732
BseMII	CTCAG	1 cut(s)	1247
BseNI	ACTGG	5 cut(s)	162, 355, 440, 836, 985
BseRI	GAGGAG	2 cut(s)	651, 1146
BseXI	GCAGC	2 cut(s)	793, 1309
BseYI	CCCAGC	1 cut(s)	75
BsgI	GTGCAG	1 cut(s)	1395
BshFI	GGCC	1 cut(s)	880
BshNI	GGYRCC	1 cut(s)	1019
BshTI	ACCGGT	1 cut(s)	17
BshVI	ATCGAT	1 cut(s)	234
BsiSI	CCGG	4 cut(s)	18, 1023, 1332, 1401
BslFI	GGGAC	1 cut(s)	422
BslI	CCNNNNNNNGG	5 cut(s)	446, 887, 995, 1174, 1178
BsmAI	GTCTC	1 cut(s)	1226
BsmFI	GGGAC	1 cut(s)	422
BsmI	GAATGC	3 cut(s)	345, 427, 1218
BsnI	GGCC	1 cut(s)	880
Bsp143I	GATC	5 cut(s)	316, 325, 832, 1099, 1259
BspACI	CCGC	2 cut(s)	1090, 1325
BspANI	GGCC	1 cut(s)	880
BspCNI	CTCAG	1 cut(s)	1248
BspDI	ATCGAT	1 cut(s)	234
BspHI	TCATGA	1 cut(s)	223
BspLI	GGNNCC	1 cut(s)	1021
BspMAI	CTGCAG	1 cut(s)	811
BspMI	ACCTGC	1 cut(s)	568
BspPI	GGATC	2 cut(s)	324, 827
BspQI	GCTCTTC	1 cut(s)	171
BspT107I	GGYRCC	1 cut(s)	1019
BsrDI	GCAATG	2 cut(s)	462, 732
BsrFI	RCCGGY	1 cut(s)	17
BsrI	ACTGG	5 cut(s)	162, 355, 440, 836, 985
BssAI	RCCGGY	1 cut(s)	17
BssECI	CCNNGG	1 cut(s)	384
BssMI	GATC	5 cut(s)	316, 325, 832, 1099, 1259
BssNAI	GTATAC	1 cut(s)	586
BssT1I	CCWWGG	1 cut(s)	384
Bst1107I	GTATAC	1 cut(s)	586
Bst4CI	ACNGT	6 cut(s)	424, 621, 1186, 1342, 1386, 1425
Bst6I	CTCTTC	2 cut(s)	171, 669
BstC8I	GCNNGC	5 cut(s)	345, 406, 1044, 1251, 1410
BstDEI	CTNAG	2 cut(s)	1245, 1256
BstEII	GGTNACC	1 cut(s)	506
BstF5I	GGATG	2 cut(s)	166, 949
BstH2I	RGCGCY	1 cut(s)	1043
BstHHI	GCGC	2 cut(s)	494, 1042
BstKTI	GATC	5 cut(s)	319, 328, 835, 1102, 1262
BstMAI	GTCTC	1 cut(s)	1226
BstMBI	GATC	5 cut(s)	316, 325, 832, 1099, 1259
BstMWI	GCNNNNNNNGC	4 cut(s)	353, 1028, 1037, 1052
BstNSI	RCATGY	2 cut(s)	584, 1253
BstPI	GGTNACC	1 cut(s)	506
BstSFI	CTRYAG	1 cut(s)	807
BstV1I	GCAGC	2 cut(s)	793, 1309
BstX2I	RGATCY	2 cut(s)	316, 325
BstXI	CCANNNNNNTGG	1 cut(s)	385
BstYI	RGATCY	2 cut(s)	316, 325
BstZ17I	GTATAC	1 cut(s)	586
Bsu15I	ATCGAT	1 cut(s)	234
BsuI	GTATCC	1 cut(s)	477
BsuRI	GGCC	1 cut(s)	880
BsuTUI	ATCGAT	1 cut(s)	234
BtgZI	GCGATG	1 cut(s)	191
BtsCI	GGATG	2 cut(s)	166, 949
BtsI	GCAGTG	1 cut(s)	378
BtsIMutI	CAGTG	3 cut(s)	348, 378, 978
BveI	ACCTGC	1 cut(s)	568
Cac8I	GCNNGC	5 cut(s)	345, 406, 1044, 1251, 1410
CciI	TCATGA	1 cut(s)	223
CfoI	GCGC	2 cut(s)	494, 1042
Cfr10I	RCCGGY	1 cut(s)	17
ClaI	ATCGAT	1 cut(s)	234
Csp6I	GTAC	4 cut(s)	12, 100, 522, 750
CspAI	ACCGGT	1 cut(s)	17
CviAII	CATG	5 cut(s)	224, 581, 962, 1015, 1250
CviQI	GTAC	4 cut(s)	12, 100, 522, 750
DdeI	CTNAG	2 cut(s)	1245, 1256
DpnI	GATC	5 cut(s)	318, 327, 834, 1101, 1261
DpnII	GATC	5 cut(s)	316, 325, 832, 1099, 1259
DraI	TTTAAA	1 cut(s)	268
Eam1104I	CTCTTC	2 cut(s)	171, 669
EarI	CTCTTC	2 cut(s)	171, 669
Eco130I	CCWWGG	1 cut(s)	384
Eco91I	GGTNACC	1 cut(s)	506
EcoO65I	GGTNACC	1 cut(s)	506
EcoRI	GAATTC	2 cut(s)	227, 789
EcoT14I	CCWWGG	1 cut(s)	384
ErhI	CCWWGG	1 cut(s)	384
FaeI	CATG	5 cut(s)	227, 584, 965, 1018, 1253
FaqI	GGGAC	1 cut(s)	422
FatI	CATG	5 cut(s)	223, 580, 961, 1014, 1249
FauNDI	CATATG	1 cut(s)	1378
FblI	GTMKAC	1 cut(s)	585
Fnu4HI	GCNGC	2 cut(s)	807, 1323
FokI	GGATG	2 cut(s)	173, 956
Fsp4HI	GCNGC	2 cut(s)	807, 1323
FspBI	CTAG	5 cut(s)	108, 336, 480, 1071, 1305
FspI	TGCGCA	1 cut(s)	493
GlaI	GCGC	2 cut(s)	493, 1041
GluI	GCNGC	2 cut(s)	807, 1323
GsaI	CCCAGC	1 cut(s)	79
GsuI	CTGGAG	1 cut(s)	372
HaeII	RGCGCY	1 cut(s)	1043
HaeIII	GGCC	1 cut(s)	880
HapII	CCGG	4 cut(s)	18, 1023, 1332, 1401
HhaI	GCGC	2 cut(s)	494, 1042
Hin1II	CATG	5 cut(s)	227, 584, 965, 1018, 1253
Hin6I	GCGC	2 cut(s)	492, 1040
HinP1I	GCGC	2 cut(s)	492, 1040
HincII	GTYRAC	1 cut(s)	862
HindII	GTYRAC	1 cut(s)	862
HinfI	GANTC	5 cut(s)	236, 530, 681, 777, 1328
HpaI	GTTAAC	1 cut(s)	862
HpaII	CCGG	4 cut(s)	18, 1023, 1332, 1401
HphI	GGTGA	4 cut(s)	152, 473, 978, 1390
Hpy166II	GTNNAC	7 cut(s)	524, 586, 862, 959, 986, 1288, 1398
Hpy188I	TCNGA	7 cut(s)	54, 91, 323, 417, 596, 1211, 1257
Hpy188III	TCNNGA	5 cut(s)	108, 224, 296, 534, 774
Hpy8I	GTNNAC	7 cut(s)	524, 586, 862, 959, 986, 1288, 1398
HpyAV	CCTTC	2 cut(s)	671, 1057
HpyCH4III	ACNGT	6 cut(s)	424, 621, 1186, 1342, 1386, 1425
HpyCH4IV	ACGT	1 cut(s)	399
HpyF10VI	GCNNNNNNNGC	4 cut(s)	353, 1028, 1037, 1052
HpyF3I	CTNAG	2 cut(s)	1245, 1256
HpySE526I	ACGT	1 cut(s)	399
Hsp92II	CATG	5 cut(s)	227, 584, 965, 1018, 1253
HspAI	GCGC	2 cut(s)	492, 1040
KspAI	GTTAAC	1 cut(s)	862
Kzo9I	GATC	5 cut(s)	316, 325, 832, 1099, 1259
LguI	GCTCTTC	1 cut(s)	171
LmnI	GCTCC	5 cut(s)	181, 188, 353, 854, 1067
Lsp1109I	GCAGC	2 cut(s)	793, 1309
LweI	GCATC	5 cut(s)	151, 378, 1126, 1270, 1350
MaeI	CTAG	5 cut(s)	108, 336, 480, 1071, 1305
MaeII	ACGT	1 cut(s)	399
MaeIII	GTNAC	3 cut(s)	506, 894, 1336
MalI	GATC	5 cut(s)	318, 327, 834, 1101, 1261
MboI	GATC	5 cut(s)	316, 325, 832, 1099, 1259
MboII	GAAGA	3 cut(s)	158, 273, 656
MflI	RGATCY	2 cut(s)	316, 325
MlyI	GAGTC	1 cut(s)	1337
MmeI	TCCRAC	3 cut(s)	766, 1108, 1189
MroXI	GAANNNNTTC	1 cut(s)	541
MseI	TTAA	4 cut(s)	267, 813, 861, 905
MspI	CCGG	4 cut(s)	18, 1023, 1332, 1401
Mva1269I	GAATGC	3 cut(s)	345, 427, 1218
MwoI	GCNNNNNNNGC	4 cut(s)	353, 1028, 1037, 1052
NdeI	CATATG	1 cut(s)	1378
NdeII	GATC	5 cut(s)	316, 325, 832, 1099, 1259
NlaIII	CATG	5 cut(s)	227, 584, 965, 1018, 1253
NlaIV	GGNNCC	1 cut(s)	1021
NmuCI	GTSAC	1 cut(s)	1336
NsbI	TGCGCA	1 cut(s)	493
NspI	RCATGY	2 cut(s)	584, 1253
PaeI	GCATGC	1 cut(s)	1253
PagI	TCATGA	1 cut(s)	223
PciI	ACATGT	1 cut(s)	580
PciSI	GCTCTTC	1 cut(s)	171
PctI	GAATGC	3 cut(s)	345, 427, 1218
PdmI	GAANNNNTTC	1 cut(s)	541
PfeI	GAWTC	4 cut(s)	236, 530, 681, 777
PflFI	GACNNNGTC	1 cut(s)	362
PflMI	CCANNNNNTGG	1 cut(s)	887
PinAI	ACCGGT	1 cut(s)	17
PkrI	GCNGC	2 cut(s)	808, 1324
PleI	GAGTC	1 cut(s)	1336
PpsI	GAGTC	1 cut(s)	1336
PscI	ACATGT	1 cut(s)	580
PsiI	TTATAA	1 cut(s)	38
PspEI	GGTNACC	1 cut(s)	506
PspFI	CCCAGC	1 cut(s)	75
PspN4I	GGNNCC	1 cut(s)	1021
PstI	CTGCAG	1 cut(s)	811
PsuI	RGATCY	2 cut(s)	316, 325
PsyI	GACNNNGTC	1 cut(s)	362
RsaI	GTAC	4 cut(s)	13, 101, 523, 751
RsaNI	GTAC	4 cut(s)	12, 100, 522, 750
SapI	GCTCTTC	1 cut(s)	171
SaqAI	TTAA	4 cut(s)	267, 813, 861, 905
SatI	GCNGC	2 cut(s)	807, 1323
Sau3AI	GATC	5 cut(s)	316, 325, 832, 1099, 1259
ScaI	AGTACT	1 cut(s)	13
SchI	GAGTC	1 cut(s)	1337
SfaNI	GCATC	5 cut(s)	151, 378, 1126, 1270, 1350
SfcI	CTRYAG	1 cut(s)	807
SmlI	CTYRAG	1 cut(s)	432
SmoI	CTYRAG	1 cut(s)	432
SphI	GCATGC	1 cut(s)	1253
SsiI	CCGC	2 cut(s)	1090, 1325
SspI	AATATT	1 cut(s)	1225
SspMI	CTAG	5 cut(s)	108, 336, 480, 1071, 1305
StyI	CCWWGG	1 cut(s)	384
TaaI	ACNGT	6 cut(s)	424, 621, 1186, 1342, 1386, 1425
TaiI	ACGT	1 cut(s)	402
TatI	WGTACW	2 cut(s)	11, 99
TfiI	GAWTC	4 cut(s)	236, 530, 681, 777
Tru1I	TTAA	4 cut(s)	267, 813, 861, 905
Tru9I	TTAA	4 cut(s)	267, 813, 861, 905
TscAI	CASTG	3 cut(s)	355, 385, 985
TseFI	GTSAC	1 cut(s)	1336
TseI	GCWGC	2 cut(s)	806, 1322
Tsp45I	GTSAC	1 cut(s)	1336
TspDTI	ATGAA	5 cut(s)	159, 220, 240, 690, 1395
TspGWI	ACGGA	1 cut(s)	737
TspRI	CASTG	3 cut(s)	355, 385, 985
Tth111I	GACNNNGTC	1 cut(s)	362
Van91I	CCANNNNNTGG	1 cut(s)	887
XapI	RAATTY	5 cut(s)	227, 497, 739, 789, 1162
XbaI	TCTAGA	1 cut(s)	107
XceI	RCATGY	2 cut(s)	584, 1253
XmiI	GTMKAC	1 cut(s)	585
XmnI	GAANNNNTTC	1 cut(s)	541
XspI	CTAG	5 cut(s)	108, 336, 480, 1071, 1305
ZrmI	AGTACT	1 cut(s)	13

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.