Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004274

Physical Location & Seq

Genomic Coordinates Reverse (-)

28662543 .. 28667386

Gene Structure 4844 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1132600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 642 bp

ATGGGTTGCTTTAGTTCTAAGGCAAGGCGGCAGTTTCCTGGACACGAGGATCCCGTGAGTCTTGCTTCGCAAACGGCTTTTACTGTTAGTGAAGTTGAAGCACTCTTTGAGTTATATAAGAGTATCAGCAGTTCTGTGATTGATGATGGGTTGATAAACAAGGAAGAATTTCAGCTAGCCATTTTCAAGAATAGAAAGACAGAAAATCTCTTTGCAAATCGGATATTTGATATGTTTGATGTTAAACGGAAGGGTGTGATTGACTTCACCGACTTTGTGAGATCACTCAATGTTTTCCATCCCAATGCCTCGCTAGAAGACAAGATTGACTTTTCATTCAAGCTTTATGATTTGGATAGCACGGGCTTTATTGAGCGCCAAGAGGTCAAACAAATGTTAATTGCACTTCTTTGTGAATCTGAAATGAAGCTGGCTGATGAGACCATTGAGATAATTCTCGATAAGACTTTCTTGGAAGCTGACGTAAATCAAGATGGGAAGATAGACAAGTATGAATGGAATAAATTTGTCTCAAAGAATCCATCATTGTTAAAGATCATGACCCTCCCCTATCTTCGGGATATTACTACCACATTTCCAAGTTTTGTATTTAATTCCGAGGTTGATGAGATCGCTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010033 GO:0010035 GO:0016020 GO:0016043 GO:0016049 GO:0019722 GO:0019899 GO:0019900 GO:0019932 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0035556 GO:0040007 GO:0042221 GO:0042995 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0044706 GO:0046872 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071840 GO:0071944 GO:0090406 GO:0097305 GO:0097306 GO:0120025 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04010 ko04020 ko04022 ko04114 ko04218 ko04310 ko04360 ko04370 ko04380 ko04650 ko04658 ko04659 ko04660 ko04662 ko04720 ko04724 ko04921 ko04922 ko04924 ko05010 ko05014 ko05031 ko05152 ko05166 ko05167 map04010 map04020 map04022 map04114 map04218 map04310 map04360 map04370 map04380 map04650 map04658 map04659 map04660 map04662 map04720 map04724 map04921 map04922 map04924 map05010 map05014 map05031 map05152 map05166 map05167

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

214

Amino Acids

24.52

Weight (kDa)

4.75

Isoelectric Point (pI)

41.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	106 - 172	2.5e-08	EF-hand domain pair
EF-hand_5	PF13202	153 - 177	4.9e-06	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004274)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17615 AT4G17615 AT4G17615 AT4G17615 AT4G17615 AT5G47100 AT5G47100
fragaria_vesca	FvH4_5g20510 FvH4_5g20510 FvH4_5g20510
malus_domestica	MD00G1132600.v1.1 MD06G1046100.v1.1
prunus_persica	Prupe.5G054400_v2.0.a1 Prupe.5G054400_v2.0.a1 Prupe.5G054400_v2.0.a1 Prupe.5G054400_v2.0.a1
pyrus_communis	pycom04g04760 pycom06g03900
rosa_chinensis	RchiOBHm_Chr7g0205861
rosa_laevigata	RLG00000003349 RLG00000014757 RLG00000017025 RLG00000018598
rosa_multiflora	Rmu_sc0000076.1_g000035 Rmu_sc0004084.1_g000001
rosa_roxburghii	Rroxscaffold_3G00251910
rosa_rugosa	Rorug07G0093500 Rorug07G0093600
rosa_samantha	Rh7BG220300 Rh7CG238800 Rh7DG231900
rosa_wichuraiana	Rw7G019420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	28
AclWI	GGATC	2 cut(s)	44, 57
AcsI	RAATTY	2 cut(s)	167, 524
AfiI	CCNNNNNNNGG	1 cut(s)	576
AgsI	TTSAA	3 cut(s)	98, 187, 340
AjnI	CCWGG	1 cut(s)	37
AluBI	AGCT	4 cut(s)	175, 343, 430, 479
AluI	AGCT	4 cut(s)	175, 343, 430, 479
Alw26I	GTCTC	2 cut(s)	434, 535
AlwI	GGATC	2 cut(s)	44, 57
ApoI	RAATTY	2 cut(s)	167, 524
Asp700I	GAANNNNTTC	1 cut(s)	168
AspLEI	GCGC	1 cut(s)	378
AsuHPI	GGTGA	1 cut(s)	259
AsuNHI	GCTAGC	1 cut(s)	175
BamHI	GGATCC	1 cut(s)	49
BauI	CACGAG	1 cut(s)	44
BbsI	GAAGAC	1 cut(s)	324
BccI	CCATC	4 cut(s)	140, 306, 488, 550
BceAI	ACGGC	1 cut(s)	90
BciT130I	CCWGG	1 cut(s)	39
BcoDI	GTCTC	2 cut(s)	434, 535
BfaI	CTAG	2 cut(s)	176, 314
BfoI	RGCGCY	1 cut(s)	379
BisI	GCNGC	1 cut(s)	29
BlsI	GCNGC	1 cut(s)	30
Bme1390I	CCNGG	1 cut(s)	39
BmiI	GGNNCC	1 cut(s)	51
BmrFI	CCNGG	1 cut(s)	39
BmtI	GCTAGC	1 cut(s)	179
BpiI	GAAGAC	1 cut(s)	324
BsaI	GGTCTC	1 cut(s)	434
BsaJI	CCNNGG	1 cut(s)	618
Bsc4I	CCNNNNNNNGG	1 cut(s)	576
BseBI	CCWGG	1 cut(s)	39
BseDI	CCNNGG	1 cut(s)	618
BseGI	GGATG	1 cut(s)	298
BseLI	CCNNNNNNNGG	1 cut(s)	576
BslI	CCNNNNNNNGG	1 cut(s)	576
BsmAI	GTCTC	2 cut(s)	434, 535
Bso31I	GGTCTC	1 cut(s)	434
Bsp143I	GATC	4 cut(s)	49, 281, 555, 630
BspACI	CCGC	1 cut(s)	28
BspHI	TCATGA	1 cut(s)	558
BspLI	GGNNCC	1 cut(s)	51
BspOI	GCTAGC	1 cut(s)	179
BspPI	GGATC	2 cut(s)	44, 57
BspTNI	GGTCTC	1 cut(s)	434
BssECI	CCNNGG	1 cut(s)	618
BssMI	GATC	4 cut(s)	49, 281, 555, 630
BssSI	CACGAG	1 cut(s)	44
Bst2BI	CACGAG	1 cut(s)	44
Bst2UI	CCWGG	1 cut(s)	39
Bst4CI	ACNGT	1 cut(s)	85
BstC8I	GCNNGC	2 cut(s)	177, 432
BstDEI	CTNAG	1 cut(s)	18
BstF5I	GGATG	1 cut(s)	298
BstH2I	RGCGCY	1 cut(s)	379
BstHHI	GCGC	1 cut(s)	378
BstKTI	GATC	4 cut(s)	52, 284, 558, 633
BstMAI	GTCTC	2 cut(s)	434, 535
BstMBI	GATC	4 cut(s)	49, 281, 555, 630
BstNI	CCWGG	1 cut(s)	39
BstSCI	CCNGG	1 cut(s)	37
BstV2I	GAAGAC	1 cut(s)	324
BstX2I	RGATCY	1 cut(s)	49
BstYI	RGATCY	1 cut(s)	49
BtsCI	GGATG	1 cut(s)	298
Cac8I	GCNNGC	2 cut(s)	177, 432
CciI	TCATGA	1 cut(s)	558
CfoI	GCGC	1 cut(s)	378
CviAII	CATG	2 cut(s)	559, 639
CviJI	RGCY	8 cut(s)	77, 175, 179, 343, 366, 430, 434, 479
CviKI_1	RGCY	8 cut(s)	77, 175, 179, 343, 366, 430, 434, 479
DdeI	CTNAG	1 cut(s)	18
DpnI	GATC	4 cut(s)	51, 283, 557, 632
DpnII	GATC	4 cut(s)	49, 281, 555, 630
Eco31I	GGTCTC	1 cut(s)	434
EcoRII	CCWGG	1 cut(s)	37
FaeI	CATG	2 cut(s)	562, 642
FaiI	YATR	7 cut(s)	115, 117, 233, 348, 513, 560, 640
FalI	AAGNNNNNCTT	4 cut(s)	314, 346, 455, 487
FatI	CATG	2 cut(s)	558, 638
Fnu4HI	GCNGC	1 cut(s)	29
FokI	GGATG	1 cut(s)	285
Fsp4HI	GCNGC	1 cut(s)	29
FspBI	CTAG	2 cut(s)	176, 314
GlaI	GCGC	1 cut(s)	377
GluI	GCNGC	1 cut(s)	29
HaeII	RGCGCY	1 cut(s)	379
HhaI	GCGC	1 cut(s)	378
Hin1II	CATG	2 cut(s)	562, 642
Hin6I	GCGC	1 cut(s)	376
HinP1I	GCGC	1 cut(s)	376
HindIII	AAGCTT	1 cut(s)	341
HinfI	GANTC	3 cut(s)	58, 416, 538
HphI	GGTGA	1 cut(s)	259
Hpy188I	TCNGA	3 cut(s)	222, 421, 619
Hpy188III	TCNNGA	5 cut(s)	187, 458, 491, 559, 578
HpyAV	CCTTC	1 cut(s)	244
HpyCH4III	ACNGT	1 cut(s)	85
HpyCH4IV	ACGT	1 cut(s)	483
HpyCH4V	TGCA	2 cut(s)	215, 404
HpyF3I	CTNAG	1 cut(s)	18
HpySE526I	ACGT	1 cut(s)	483
Hsp92II	CATG	2 cut(s)	562, 642
HspAI	GCGC	1 cut(s)	376
Kzo9I	GATC	4 cut(s)	49, 281, 555, 630
LpnPI	CCDG	3 cut(s)	24, 51, 416
MaeI	CTAG	2 cut(s)	176, 314
MaeII	ACGT	1 cut(s)	483
MalI	GATC	4 cut(s)	51, 283, 557, 632
MboI	GATC	4 cut(s)	49, 281, 555, 630
MboII	GAAGA	4 cut(s)	176, 329, 511, 566
MflI	RGATCY	1 cut(s)	49
MluCI	AATT	5 cut(s)	167, 399, 453, 524, 613
MlyI	GAGTC	1 cut(s)	67
MnlI	CCTC	5 cut(s)	40, 319, 376, 575, 613
MroXI	GAANNNNTTC	1 cut(s)	168
MseI	TTAA	4 cut(s)	243, 398, 551, 612
MslI	CAYNNNNRTG	1 cut(s)	303
MspR9I	CCNGG	1 cut(s)	39
MvaI	CCWGG	1 cut(s)	39
NdeII	GATC	4 cut(s)	49, 281, 555, 630
NheI	GCTAGC	1 cut(s)	175
NlaIII	CATG	2 cut(s)	562, 642
NlaIV	GGNNCC	1 cut(s)	51
PagI	TCATGA	1 cut(s)	558
PcsI	WCGNNNNNNNCGW	1 cut(s)	51
PdmI	GAANNNNTTC	1 cut(s)	168
PfeI	GAWTC	2 cut(s)	416, 538
PfoI	TCCNGGA	1 cut(s)	37
PkrI	GCNGC	1 cut(s)	30
PleI	GAGTC	1 cut(s)	66
PpsI	GAGTC	1 cut(s)	66
Psp6I	CCWGG	1 cut(s)	37
PspGI	CCWGG	1 cut(s)	37
PspN4I	GGNNCC	1 cut(s)	51
PsrI	GAACNNNNNNTAC	2 cut(s)	115, 147
PsuI	RGATCY	1 cut(s)	49
RseI	CAYNNNNRTG	1 cut(s)	303
SaqAI	TTAA	4 cut(s)	243, 398, 551, 612
SatI	GCNGC	1 cut(s)	29
Sau3AI	GATC	4 cut(s)	49, 281, 555, 630
SchI	GAGTC	1 cut(s)	67
ScrFI	CCNGG	1 cut(s)	39
SetI	ASST	7 cut(s)	177, 345, 387, 432, 481, 486, 624
SmiMI	CAYNNNNRTG	1 cut(s)	303
Sse9I	AATT	5 cut(s)	167, 399, 453, 524, 613
SsiI	CCGC	1 cut(s)	28
SspMI	CTAG	2 cut(s)	176, 314
StyD4I	CCNGG	1 cut(s)	37
TaaI	ACNGT	1 cut(s)	85
TaiI	ACGT	1 cut(s)	486
TaqI	TCGA	1 cut(s)	459
TasI	AATT	5 cut(s)	167, 399, 453, 524, 613
TauI	GCSGC	1 cut(s)	31
TfiI	GAWTC	2 cut(s)	416, 538
Tru1I	TTAA	4 cut(s)	243, 398, 551, 612
Tru9I	TTAA	4 cut(s)	243, 398, 551, 612
TspDTI	ATGAA	3 cut(s)	324, 440, 528
TspGWI	ACGGA	1 cut(s)	262
XapI	RAATTY	2 cut(s)	167, 524
XmnI	GAANNNNTTC	1 cut(s)	168
XspI	CTAG	2 cut(s)	176, 314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.