RLG00000003349

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004274
Physical Location & Seq
Reverse (-)
47478270 .. 47483043
4774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003349

Sequence Viewer

Length: 711 bp
ATGAGCTTTAGGAGTTTGTTCTACCTCTGCTTTCCTCTCCTCGTCTCTGTTTCTGTTGCATTTTCGTCCGACCTCAGTTGGCCGCAGGCTGAGATTTTCCGGCCAAATTCTTGGTCGGGAAGCTTTCCAGGATACGAGGATCCTGTCTCCCTCGCTTCACAAACAGCATATGTGAACTGCTTGTTTCAACTATCTTGCACGCAACCTCATACTGTTTTTAACAATTGTCTTGAGGAGTTCCAGTTAGCCATTTTCAAGAATAGAAAGAAAGAAAATTTGTTTGCAAATCGGATATTTGATCTATTTGATGTGAAGAGGAAGGGTGTCATTGATTTCAATGACTTTGTTAGATCACTGACTGTCTTCCATCCCAATGCCCCACAAGAAGACAAGATCGATTTTTCATTTAAGCTCTATGACTTGGATAACACGGGCTTTATTGAGCGCCAAGAGGTCAAGCAAATGTTAATTGCACTTCTTTGTGAATCTGAAATGAAACTGGCAGATGACACCATCGAGTTAATTCTTGATAAGACTTTCATGGAAGCTGATGTGAACCAAGATGGGAAAATAGACTTTTCTGAGTGGAAAAATTTTGTTTCAAAGAATCCATCATTGTTAAAGATCATGACCCTCCCCTACCTTAGGGATATTACCACCACATTTCCCAGTTTTGTATTTAATTCAGAGGTTGATGAGATTGCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010033 GO:0010035 GO:0016020 GO:0016043 GO:0016049 GO:0019722 GO:0019899 GO:0019900 GO:0019932 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0035556 GO:0040007 GO:0042221 GO:0042995 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0044706 GO:0046872 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071840 GO:0071944 GO:0090406 GO:0097305 GO:0097306 GO:0120025 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04010 ko04020 ko04022 ko04114 ko04218 ko04310 ko04360 ko04370 ko04380 ko04650 ko04658 ko04659 ko04660 ko04662 ko04720 ko04724 ko04921 ko04922 ko04924 ko05010 ko05014 ko05031 ko05152 ko05166 ko05167 map04010 map04020 map04022 map04114 map04218 map04310 map04360 map04370 map04380 map04650 map04658 map04659 map04660 map04662 map04720 map04724 map04921 map04922 map04924 map05010 map05014 map05031 map05152 map05166 map05167
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

237

Amino Acids

27.28

Weight (kDa)

4.68

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 129 - 199 3.4e-10 EF-hand domain pair
EF-hand_1 PF00036 176 - 202 4.1e-07 EF hand domain
EF-hand_5 PF13202 176 - 200 8.4e-08 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 83
AclWI GGATC 2 cut(s) 134, 147
AcoI YGGCCR 2 cut(s) 80, 101
AcsI RAATTY 3 cut(s) 106, 274, 592
AfiI CCNNNNNNNGG 1 cut(s) 645
AgsI TTSAA 4 cut(s) 188, 256, 337, 603
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 4 cut(s) 6, 123, 412, 548
AluI AGCT 4 cut(s) 6, 123, 412, 548
Alw26I GTCTC 2 cut(s) 49, 151
AlwI GGATC 2 cut(s) 134, 147
AoxI GGCC 2 cut(s) 80, 101
ApoI RAATTY 3 cut(s) 106, 274, 592
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AspLEI GCGC 1 cut(s) 447
AxyI CCTNAGG 1 cut(s) 644
BamHI GGATCC 1 cut(s) 139
BbsI GAAGAC 2 cut(s) 355, 393
BccI CCATC 4 cut(s) 375, 521, 557, 619
BciT130I CCWGG 1 cut(s) 129
BciVI GTATCC 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 49, 151
BfoI RGCGCY 1 cut(s) 448
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 129
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 1 cut(s) 129
BmrI ACTGGG 1 cut(s) 663
BmuI ACTGGG 1 cut(s) 663
BpiI GAAGAC 2 cut(s) 355, 393
BpuEI CTTGAG 1 cut(s) 251
Bsa29I ATCGAT 1 cut(s) 396
BsaAI YACGTR 1 cut(s) 708
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 645
Bse1I ACTGG 3 cut(s) 241, 504, 669
Bse21I CCTNAGG 1 cut(s) 644
BseBI CCWGG 1 cut(s) 129
BseCI ATCGAT 1 cut(s) 396
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 645
BseMII CTCAG 3 cut(s) 81, 88, 573
BseNI ACTGG 3 cut(s) 241, 504, 669
BseRI GAGGAG 2 cut(s) 29, 248
BshFI GGCC 2 cut(s) 82, 103
BshVI ATCGAT 1 cut(s) 396
BsiSI CCGG 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 645
BsmAI GTCTC 2 cut(s) 49, 151
BsmBI CGTCTC 1 cut(s) 49
BsnI GGCC 2 cut(s) 82, 103
Bsp143I GATC 5 cut(s) 139, 298, 350, 393, 624
BspACI CCGC 1 cut(s) 83
BspANI GGCC 2 cut(s) 82, 103
BspCNI CTCAG 3 cut(s) 82, 87, 574
BspDI ATCGAT 1 cut(s) 396
BspHI TCATGA 1 cut(s) 627
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 2 cut(s) 134, 147
BsrI ACTGG 3 cut(s) 241, 504, 669
BssMI GATC 5 cut(s) 139, 298, 350, 393, 624
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 214, 361
Bst6I CTCTTC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 708
BstC8I GCNNGC 2 cut(s) 87, 200
BstDEI CTNAG 4 cut(s) 74, 90, 582, 644
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 5 cut(s) 142, 301, 353, 396, 627
BstMAI GTCTC 2 cut(s) 49, 151
BstMBI GATC 5 cut(s) 139, 298, 350, 393, 624
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstV2I GAAGAC 2 cut(s) 355, 393
BstX2I RGATCY 1 cut(s) 139
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 1 cut(s) 139
Bsu15I ATCGAT 1 cut(s) 396
Bsu36I CCTNAGG 1 cut(s) 644
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 82, 103
BsuTUI ATCGAT 1 cut(s) 396
BtsCI GGATG 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 87, 200
CciI TCATGA 1 cut(s) 627
CfoI GCGC 1 cut(s) 447
ClaI ATCGAT 1 cut(s) 396
CviAII CATG 2 cut(s) 541, 628
CviJI RGCY 9 cut(s) 6, 82, 89, 103, 123, 248, 412, 435, 548
CviKI_1 RGCY 9 cut(s) 6, 82, 89, 103, 123, 248, 412, 435, 548
DdeI CTNAG 4 cut(s) 74, 90, 582, 644
DpnI GATC 5 cut(s) 141, 300, 352, 395, 626
DpnII GATC 5 cut(s) 139, 298, 350, 393, 624
EaeI YGGCCR 2 cut(s) 80, 101
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco81I CCTNAGG 1 cut(s) 644
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 2 cut(s) 544, 631
FaiI YATR 6 cut(s) 169, 171, 210, 417, 542, 629
FatI CATG 2 cut(s) 540, 627
FauNDI CATATG 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 83
GlaI GCGC 1 cut(s) 446
GluI GCNGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 2 cut(s) 82, 103
HapII CCGG 1 cut(s) 100
HhaI GCGC 1 cut(s) 447
Hin1II CATG 2 cut(s) 544, 631
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 2 cut(s) 485, 607
HpaII CCGG 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 175, 556
Hpy188I TCNGA 5 cut(s) 70, 291, 490, 583, 688
Hpy188III TCNNGA 5 cut(s) 117, 230, 256, 527, 628
Hpy8I GTNNAC 2 cut(s) 175, 556
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 2 cut(s) 214, 361
HpyCH4IV ACGT 1 cut(s) 707
HpyCH4V TGCA 4 cut(s) 59, 198, 284, 473
HpyF3I CTNAG 4 cut(s) 74, 90, 582, 644
HpySE526I ACGT 1 cut(s) 707
Hsp92II CATG 2 cut(s) 544, 631
HspAI GCGC 1 cut(s) 445
Kzo9I GATC 5 cut(s) 139, 298, 350, 393, 624
LpnPI CCDG 8 cut(s) 71, 113, 114, 141, 156, 254, 485, 682
MaeII ACGT 1 cut(s) 707
MalI GATC 5 cut(s) 141, 300, 352, 395, 626
MboI GATC 5 cut(s) 139, 298, 350, 393, 624
MboII GAAGA 3 cut(s) 325, 355, 398
MfeI CAATTG 1 cut(s) 223
MflI RGATCY 1 cut(s) 139
MluCI AATT 7 cut(s) 106, 223, 274, 468, 522, 592, 682
MmeI TCCRAC 1 cut(s) 93
MseI TTAA 6 cut(s) 219, 408, 467, 521, 620, 681
MslI CAYNNNNRTG 1 cut(s) 372
MspI CCGG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 169
NdeII GATC 5 cut(s) 139, 298, 350, 393, 624
NlaIII CATG 2 cut(s) 544, 631
NlaIV GGNNCC 1 cut(s) 141
PagI TCATGA 1 cut(s) 627
PfeI GAWTC 2 cut(s) 485, 607
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 141
PsuI RGATCY 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 6 cut(s) 219, 408, 467, 521, 620, 681
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 5 cut(s) 139, 298, 350, 393, 624
ScrFI CCNGG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 7 cut(s) 106, 223, 274, 468, 522, 592, 682
SsiI CCGC 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 2 cut(s) 214, 361
TaiI ACGT 1 cut(s) 710
TaqI TCGA 2 cut(s) 396, 516
TasI AATT 7 cut(s) 106, 223, 274, 468, 522, 592, 682
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 2 cut(s) 485, 607
Tru1I TTAA 6 cut(s) 219, 408, 467, 521, 620, 681
Tru9I TTAA 6 cut(s) 219, 408, 467, 521, 620, 681
TscAI CASTG 1 cut(s) 360
TspDTI ATGAA 3 cut(s) 393, 509, 529
TspRI CASTG 1 cut(s) 360
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 3 cut(s) 106, 274, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.