MD00G1142400.v1.1

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007690
Physical Location & Seq
Reverse (-)
30993049 .. 30995600
2552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1142400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGAATAGCACTGGAAGAAACAGCCGGTTTCCTTTCACTCCAACTCAGTGGCAAGAGCTTGAGCACCAAGCTCTTATCTACAAGTACATGGTTTCTGGAATCTCCATCCCTCCTGATCTCCTCTTCTCCCTCAAAAGAAGCACCTACAACTTGGACTCCCCTCTCCCTCCAAAGCTCTTCTCTCACCACCCTCCTCACATTGGATGGAACTGTTTCCAGATGGGATTAGGGAGAAAAATAGACCCAGAGCCAGGAAGGTGCAGAAGAACAGACGGGAAAAAATGGAGGTGCTCAAAAGAGGCGTTTCCGGATTCGAAATACTGCGAGAAGCACATGCACAGAGGGAAAAACCGTTCAAGAAAGCCTGTGGAAGTTCTGAAAACATCTCAAATTTCAACAACAAACTCATCAGACACTTCAACAATTACCACCAGAATCAACACTTCTTCTGCTTCTCCAAGTCCTGCAGCTTTTCATTCCCTTTCATCATCACTCTCTTCTCTCTCCTCTGATTCCCACGCTTACCCTTTCCTCTATCATCATAACTCACCTTCTCCAAGGCCTACTGGAATCGGATTCCCACATCAAGAGAGAAGCACTACTTCCTTGTTTTTGGACTCTGGTTCTTATCCCCAACCCAGCTCAGATTACAGGTATGCTTATGGGATGAAAGAGGAGGTGGATGAACATGCATTCTTCTCAGAGCCTTCAGGCACAGTGAGAGGCTTCTCTGGCTCTTCATCCATGGACGATTCCTGGCAGTTCACACCGCTCACAATTAGTACTTCTTCGTCTTCGAAACAGAGAAGCTGCTCTGCTTTTCAAAGTGATCAGCAGCTTCAGAGCATCAAACATCAAAAGCAAGATCATCATCAGCAGCAGCAGCATTACTATGCTTTGGACAGAAACGAAGGATCCCAGAAGACCATTCATCGATTCTTCGACGAATGGCCCCCGAAAGATAAAGATTCATGGCTCGATTTGGATGATAAATCATCAAACAGCGGATCGGTTTCTACAACCAGGCTCTCAATCTCCATCCCTACTTCTGCTCATGACTTCCCCATCTTCACCTCAAGACATCATAATAATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

366

Amino Acids

41.39

Weight (kDa)

8.83

Isoelectric Point (pI)

61.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 11 - 44 1.7e-14 QLQ
WRC PF08879 82 - 124 1e-20 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 772
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 2 cut(s) 770, 1005
AclWI GGATC 3 cut(s) 909, 922, 1015
AcsI RAATTY 1 cut(s) 390
AcuI CTGAAG 2 cut(s) 693, 824
AfaI GTAC 2 cut(s) 86, 784
AfiI CCNNNNNNNGG 2 cut(s) 200, 251
AgsI TTSAA 4 cut(s) 357, 396, 420, 824
AjnI CCWGG 3 cut(s) 250, 755, 1022
AloI GAACNNNNNNTCC 2 cut(s) 337, 369
AluBI AGCT 7 cut(s) 58, 71, 175, 470, 642, 810, 838
AluI AGCT 7 cut(s) 58, 71, 175, 470, 642, 810, 838
Alw21I GWGCWC 2 cut(s) 66, 293
AlwI GGATC 3 cut(s) 909, 922, 1015
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 2 cut(s) 560, 950
ApeKI GCWGC 6 cut(s) 467, 810, 835, 877, 880, 883
ApoI RAATTY 1 cut(s) 390
Asp700I GAANNNNTTC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 951
AsuHPI GGTGA 3 cut(s) 176, 540, 1063
AsuII TTCGAA 2 cut(s) 314, 797
BamHI GGATCC 1 cut(s) 914
BbsI GAAGAC 2 cut(s) 786, 929
Bbv12I GWGCWC 2 cut(s) 66, 293
BbvI GCAGC 6 cut(s) 479, 797, 847, 889, 892, 895
BccI CCATC 5 cut(s) 113, 198, 214, 1046, 1073
BciT130I CCWGG 3 cut(s) 252, 757, 1024
BclI TGATCA 1 cut(s) 829
BfmI CTRYAG 1 cut(s) 465
BisI GCNGC 6 cut(s) 468, 811, 836, 878, 881, 884
BlsI GCNGC 6 cut(s) 469, 812, 837, 879, 882, 885
BmcAI AGTACT 1 cut(s) 784
Bme1390I CCNGG 3 cut(s) 252, 757, 1024
BmgT120I GGNCC 1 cut(s) 951
BmiI GGNNCC 2 cut(s) 916, 953
BmrFI CCNGG 3 cut(s) 252, 757, 1024
BmsI GCATC 1 cut(s) 855
BpiI GAAGAC 2 cut(s) 786, 929
Bpu14I TTCGAA 2 cut(s) 314, 797
BpuEI CTTGAG 2 cut(s) 80, 1060
Bsa29I ATCGAT 1 cut(s) 934
BsaBI GATNNNNATC 1 cut(s) 870
BsaJI CCNNGG 2 cut(s) 557, 744
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 251
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 16, 571
Bse8I GATNNNNATC 1 cut(s) 870
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 3 cut(s) 252, 757, 1024
BseCI ATCGAT 1 cut(s) 934
BseDI CCNNGG 2 cut(s) 557, 744
BseGI GGATG 7 cut(s) 105, 209, 672, 688, 740, 991, 1038
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 2 cut(s) 200, 251
BseMII CTCAG 3 cut(s) 59, 657, 714
BseNI ACTGG 2 cut(s) 16, 571
BseRI GAGGAG 4 cut(s) 110, 183, 496, 689
BseXI GCAGC 6 cut(s) 479, 797, 847, 889, 892, 895
BseYI CCCAGC 1 cut(s) 638
BsgI GTGCAG 1 cut(s) 280
BshFI GGCC 2 cut(s) 562, 952
BshVI ATCGAT 1 cut(s) 934
BsiHKAI GWGCWC 2 cut(s) 66, 293
BsiSI CCGG 2 cut(s) 25, 308
BslI CCNNNNNNNGG 2 cut(s) 200, 251
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 2 cut(s) 562, 952
Bsp119I TTCGAA 2 cut(s) 314, 797
Bsp1286I GDGCHC 2 cut(s) 66, 293
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 5 cut(s) 115, 829, 865, 914, 1007
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 2 cut(s) 770, 1005
BspANI GGCC 2 cut(s) 562, 952
BspCNI CTCAG 3 cut(s) 58, 656, 713
BspDI ATCGAT 1 cut(s) 934
BspEI TCCGGA 1 cut(s) 307
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 2 cut(s) 916, 953
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 3 cut(s) 909, 922, 1015
BspQI GCTCTTC 2 cut(s) 182, 742
BspT104I TTCGAA 2 cut(s) 314, 797
BsrBI CCGCTC 1 cut(s) 772
BsrFI RCCGGY 1 cut(s) 24
BsrI ACTGG 2 cut(s) 16, 571
BssAI RCCGGY 1 cut(s) 24
BssECI CCNNGG 2 cut(s) 557, 744
BssMI GATC 5 cut(s) 115, 829, 865, 914, 1007
BssT1I CCWWGG 2 cut(s) 557, 744
Bst2UI CCWGG 3 cut(s) 252, 757, 1024
Bst4CI ACNGT 3 cut(s) 212, 353, 718
Bst6I CTCTTC 4 cut(s) 128, 182, 502, 742
BstBI TTCGAA 2 cut(s) 314, 797
BstDEI CTNAG 3 cut(s) 45, 643, 700
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 7 cut(s) 105, 209, 672, 688, 740, 991, 1038
BstKTI GATC 5 cut(s) 118, 832, 868, 917, 1010
BstMBI GATC 5 cut(s) 115, 829, 865, 914, 1007
BstMWI GCNNNNNNNGC 2 cut(s) 732, 883
BstNI CCWGG 3 cut(s) 252, 757, 1024
BstNSI RCATGY 2 cut(s) 337, 692
BstSCI CCNGG 3 cut(s) 250, 755, 1022
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 6 cut(s) 479, 797, 847, 889, 892, 895
BstV2I GAAGAC 2 cut(s) 786, 929
BstX2I RGATCY 1 cut(s) 914
BstXI CCANNNNNNTGG 1 cut(s) 48
BstYI RGATCY 1 cut(s) 914
Bsu15I ATCGAT 1 cut(s) 934
BsuRI GGCC 2 cut(s) 562, 952
BsuTUI ATCGAT 1 cut(s) 934
BtgI CCRYGG 1 cut(s) 744
BtsCI GGATG 7 cut(s) 105, 209, 672, 688, 740, 991, 1038
BtsIMutI CAGTG 3 cut(s) 9, 53, 723
CciI TCATGA 1 cut(s) 1054
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 951
ClaI ATCGAT 1 cut(s) 934
Csp6I GTAC 2 cut(s) 85, 783
CviAII CATG 6 cut(s) 88, 334, 689, 745, 972, 1055
CviQI GTAC 2 cut(s) 85, 783
DdeI CTNAG 3 cut(s) 45, 643, 700
DpnI GATC 5 cut(s) 117, 831, 867, 916, 1009
DpnII GATC 5 cut(s) 115, 829, 865, 914, 1007
Eam1104I CTCTTC 4 cut(s) 128, 182, 502, 742
EarI CTCTTC 4 cut(s) 128, 182, 502, 742
Eco130I CCWWGG 2 cut(s) 557, 744
Eco147I AGGCCT 1 cut(s) 562
Eco57I CTGAAG 2 cut(s) 693, 824
EcoRII CCWGG 3 cut(s) 250, 755, 1022
EcoT14I CCWWGG 2 cut(s) 557, 744
EcoT22I ATGCAT 1 cut(s) 694
ErhI CCWWGG 2 cut(s) 557, 744
FaeI CATG 6 cut(s) 91, 337, 692, 748, 975, 1058
FalI AAGNNNNNCTT 2 cut(s) 586, 618
FatI CATG 6 cut(s) 87, 333, 688, 744, 971, 1054
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 6 cut(s) 468, 811, 836, 878, 881, 884
FokI GGATG 7 cut(s) 92, 216, 679, 695, 727, 998, 1025
Fsp4HI GCNGC 6 cut(s) 468, 811, 836, 878, 881, 884
GluI GCNGC 6 cut(s) 468, 811, 836, 878, 881, 884
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 2 cut(s) 562, 952
HapII CCGG 2 cut(s) 25, 308
Hin1II CATG 6 cut(s) 91, 337, 692, 748, 975, 1058
HpaII CCGG 2 cut(s) 25, 308
HphI GGTGA 3 cut(s) 176, 540, 1063
Hpy166II GTNNAC 1 cut(s) 765
Hpy188I TCNGA 7 cut(s) 378, 412, 511, 575, 646, 703, 843
Hpy188III TCNNGA 8 cut(s) 96, 113, 217, 308, 357, 587, 1055, 1077
Hpy8I GTNNAC 1 cut(s) 765
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 4 cut(s) 249, 561, 717, 905
HpyCH4III ACNGT 3 cut(s) 212, 353, 718
HpyCH4V TGCA 4 cut(s) 261, 337, 467, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 732, 883
HpyF3I CTNAG 3 cut(s) 45, 643, 700
Hsp92II CATG 6 cut(s) 91, 337, 692, 748, 975, 1058
Kpn2I TCCGGA 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 5 cut(s) 115, 829, 865, 914, 1007
LguI GCTCTTC 2 cut(s) 182, 742
Lsp1109I GCAGC 6 cut(s) 479, 797, 847, 889, 892, 895
LweI GCATC 1 cut(s) 855
MalI GATC 5 cut(s) 117, 831, 867, 916, 1009
MbiI CCGCTC 1 cut(s) 772
MboI GATC 5 cut(s) 115, 829, 865, 914, 1007
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 2 cut(s) 66, 293
MluCI AATT 3 cut(s) 390, 423, 777
MlyI GAGTC 2 cut(s) 149, 611
MmeI TCCRAC 1 cut(s) 65
Mph1103I ATGCAT 1 cut(s) 694
MroI TCCGGA 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 212
MslI CAYNNNNRTG 2 cut(s) 891, 1089
MspA1I CMGCKG 1 cut(s) 1005
MspI CCGG 2 cut(s) 25, 308
MspR9I CCNGG 3 cut(s) 252, 757, 1024
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 3 cut(s) 252, 757, 1024
MwoI GCNNNNNNNGC 2 cut(s) 732, 883
NcoI CCATGG 1 cut(s) 744
NdeII GATC 5 cut(s) 115, 829, 865, 914, 1007
NlaIII CATG 6 cut(s) 91, 337, 692, 748, 975, 1058
NlaIV GGNNCC 2 cut(s) 916, 953
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 2 cut(s) 337, 692
NspV TTCGAA 2 cut(s) 314, 797
PagI TCATGA 1 cut(s) 1054
PceI AGGCCT 1 cut(s) 562
PciSI GCTCTTC 2 cut(s) 182, 742
PctI GAATGC 1 cut(s) 692
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 9 cut(s) 99, 311, 435, 512, 570, 576, 752, 936, 968
PkrI GCNGC 6 cut(s) 469, 812, 837, 879, 882, 885
PleI GAGTC 2 cut(s) 149, 611
PpsI GAGTC 2 cut(s) 149, 611
Psp6I CCWGG 3 cut(s) 250, 755, 1022
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 3 cut(s) 250, 755, 1022
PspN4I GGNNCC 2 cut(s) 916, 953
PspPI GGNCC 1 cut(s) 951
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 914
RsaI GTAC 2 cut(s) 86, 784
RsaNI GTAC 2 cut(s) 85, 783
RseI CAYNNNNRTG 2 cut(s) 891, 1089
SapI GCTCTTC 2 cut(s) 182, 742
SatI GCNGC 6 cut(s) 468, 811, 836, 878, 881, 884
Sau3AI GATC 5 cut(s) 115, 829, 865, 914, 1007
Sau96I GGNCC 1 cut(s) 951
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 2 cut(s) 149, 611
ScrFI CCNGG 3 cut(s) 252, 757, 1024
SduI GDGCHC 2 cut(s) 66, 293
SfaNI GCATC 1 cut(s) 855
SfcI CTRYAG 1 cut(s) 465
SfuI TTCGAA 2 cut(s) 314, 797
SmiMI CAYNNNNRTG 2 cut(s) 891, 1089
SmlI CTYRAG 2 cut(s) 59, 1075
SmoI CTYRAG 2 cut(s) 59, 1075
Sse9I AATT 3 cut(s) 390, 423, 777
SseBI AGGCCT 1 cut(s) 562
SsiI CCGC 2 cut(s) 770, 1005
StuI AGGCCT 1 cut(s) 562
StyD4I CCNGG 3 cut(s) 250, 755, 1022
StyI CCWWGG 2 cut(s) 557, 744
TaaI ACNGT 3 cut(s) 212, 353, 718
TaqI TCGA 5 cut(s) 314, 797, 934, 942, 978
TasI AATT 3 cut(s) 390, 423, 777
TatI WGTACW 2 cut(s) 84, 782
TfiI GAWTC 9 cut(s) 99, 311, 435, 512, 570, 576, 752, 936, 968
TscAI CASTG 3 cut(s) 16, 53, 723
TseI GCWGC 6 cut(s) 467, 810, 835, 877, 880, 883
TspDTI ATGAA 8 cut(s) 17, 464, 474, 683, 699, 729, 920, 960
TspRI CASTG 3 cut(s) 16, 53, 723
XapI RAATTY 1 cut(s) 390
XceI RCATGY 2 cut(s) 337, 692
XmnI GAANNNNTTC 1 cut(s) 212
ZrmI AGTACT 1 cut(s) 784
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.