MD00G1167800.v1.1

Polypyrimidine tract-binding protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004917
Physical Location & Seq
Forward (+)
38542371 .. 38543112
742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1167800.v1.1.491

Sequence Viewer

Length: 699 bp
ATGTCGACATCGGGGACGCCGCAGTTCCGGTACACGCAGACGCCGTCGAAGGTGCTCCACCTCCATAACCTCCCGTGGGAGTGCGCCGAGGAGGAGTTGATCGAGCTCTGCAAACCTTTCGGCAAGATCGTCAACACTAAGTGTAATGTCGGCGCCAATCGCAATCAGGCCTTCGTCGAATTCTCGGACCTAAACCAGGCCATCAATATGGTTTCGTATTATGCTTCATCCTCGGAGCCTGCGCAGGTCCGTGGTAAAACCGTGTACATTCAGTATTCAAATAGACACGAAATTGTCAATAACAAAAGTCCGGGAGATGTGCCCGGAAATGTCTTGTTGGTAACCATTGAGGGTGTTGAAGCTGGCGATGTGAGCATTGATGTTATTCACTTGGTGTTCTCTGCTTTCGGCTTTGCATTGATTCAGTTCAGTGATGCTGTCACCGCATCTATGGCAAGGAATGCATTGGATGGAAGAAGTATACCCAGGTACTTGCTTCCAGAGAACGTCGGTTCATGCAACTTACGTATTTCATATTCCGCTCCCCCGATCTGCGAACACCACCGTCACTACACCACAACTGGCTTCCATGCTCCGCTCAGGTGGAGGGTCTTGGGTTCGAAGGTTGTGTGCTTCTCCTGCTCAGCAACATTTGATTCGGTATCGACAGCATCAGCAACGTCAGCGCCAGCAGCGTGA

Protein Analysis

233

Amino Acids

25.33

Weight (kDa)

6.7

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 22 - 70 1.1e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 2 cut(s) 542, 598
AccI GTMKAC 2 cut(s) 5, 481
AciI CCGC 4 cut(s) 20, 444, 540, 596
AcsI RAATTY 1 cut(s) 179
AcyI GRCGYC 3 cut(s) 17, 41, 153
AdeI CACNNNGTG 2 cut(s) 141, 394
AfaI GTAC 3 cut(s) 32, 266, 491
AfiI CCNNNNNNNGG 2 cut(s) 76, 196
AgsI TTSAA 2 cut(s) 279, 359
AjnI CCWGG 2 cut(s) 195, 485
AluBI AGCT 2 cut(s) 106, 362
AluI AGCT 2 cut(s) 106, 362
Alw21I GWGCWC 2 cut(s) 57, 108
AoxI GGCC 2 cut(s) 168, 198
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 1 cut(s) 179
AspLEI GCGC 4 cut(s) 86, 155, 244, 688
AspS9I GGNCC 2 cut(s) 187, 247
AsuC2I CCSGG 2 cut(s) 312, 324
AsuHPI GGTGA 1 cut(s) 433
AsuII TTCGAA 1 cut(s) 620
AvaII GGWCC 2 cut(s) 187, 247
BaeGI GKGCMC 1 cut(s) 324
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 57, 108
BccI CCATC 2 cut(s) 209, 464
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 2 cut(s) 197, 487
BcnI CCSGG 2 cut(s) 312, 324
BfoI RGCGCY 2 cut(s) 156, 689
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 2 cut(s) 20, 693
BlpI GCTNAGC 1 cut(s) 643
BlsI GCNGC 2 cut(s) 21, 694
Bme1390I CCNGG 4 cut(s) 197, 312, 324, 487
Bme18I GGWCC 2 cut(s) 187, 247
BmgT120I GGNCC 2 cut(s) 187, 247
BmiI GGNNCC 2 cut(s) 154, 237
BmrFI CCNGG 4 cut(s) 197, 312, 324, 487
BmsI GCATC 3 cut(s) 424, 455, 680
Bpu10I CCTNAGC 1 cut(s) 599
Bpu1102I GCTNAGC 1 cut(s) 643
Bpu14I TTCGAA 1 cut(s) 620
BpuMI CCSGG 2 cut(s) 312, 324
BsaAI YACGTR 1 cut(s) 527
BsaHI GRCGYC 3 cut(s) 17, 41, 153
BsaJI CCNNGG 5 cut(s) 74, 87, 231, 250, 485
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 196
Bse1I ACTGG 1 cut(s) 586
BseBI CCWGG 2 cut(s) 197, 487
BseDI CCNNGG 5 cut(s) 74, 87, 231, 250, 485
BseGI GGATG 2 cut(s) 227, 475
BseLI CCNNNNNNNGG 2 cut(s) 76, 196
BseMII CTCAG 2 cut(s) 613, 657
BseNI ACTGG 1 cut(s) 586
BseRI GAGGAG 2 cut(s) 104, 107
BseSI GKGCMC 1 cut(s) 324
BshFI GGCC 2 cut(s) 170, 200
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 2 cut(s) 57, 108
BsiSI CCGG 3 cut(s) 28, 311, 324
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 2 cut(s) 76, 196
BsmFI GGGAC 1 cut(s) 28
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 2 cut(s) 170, 200
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 3 cut(s) 57, 108, 324
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 3 cut(s) 99, 126, 549
Bsp1720I GCTNAGC 1 cut(s) 643
BspACI CCGC 4 cut(s) 20, 444, 540, 596
BspANI GGCC 2 cut(s) 170, 200
BspCNI CTCAG 2 cut(s) 612, 656
BspLI GGNNCC 2 cut(s) 154, 237
BspMI ACCTGC 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 620
BspT107I GGYRCC 1 cut(s) 152
BsrBI CCGCTC 2 cut(s) 542, 598
BsrGI TGTACA 1 cut(s) 264
BsrI ACTGG 1 cut(s) 586
BssECI CCNNGG 5 cut(s) 74, 87, 231, 250, 485
BssMI GATC 3 cut(s) 99, 126, 549
BssNAI GTATAC 1 cut(s) 482
BssNI GRCGYC 3 cut(s) 17, 41, 153
Bst1107I GTATAC 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 197, 487
Bst4CI ACNGT 2 cut(s) 262, 566
BstACI GRCGYC 3 cut(s) 17, 41, 153
BstAPI GCANNNNNTGC 1 cut(s) 461
BstAUI TGTACA 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 240, 364, 690
BstDEI CTNAG 3 cut(s) 138, 599, 643
BstDSI CCRYGG 2 cut(s) 74, 250
BstEII GGTNACC 1 cut(s) 340
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 2 cut(s) 227, 475
BstH2I RGCGCY 2 cut(s) 156, 689
BstHHI GCGC 4 cut(s) 86, 155, 244, 688
BstKTI GATC 3 cut(s) 102, 129, 552
BstMBI GATC 3 cut(s) 99, 126, 549
BstMWI GCNNNNNNNGC 8 cut(s) 159, 372, 443, 452, 461, 639, 683, 692
BstNI CCWGG 2 cut(s) 197, 487
BstPI GGTNACC 1 cut(s) 340
BstSCI CCNGG 4 cut(s) 195, 310, 322, 485
BstSLI GKGCMC 1 cut(s) 324
BstSNI TACGTA 1 cut(s) 527
BstXI CCANNNNNNTGG 1 cut(s) 208
BstZ17I GTATAC 1 cut(s) 482
BsuRI GGCC 2 cut(s) 170, 200
BtgI CCRYGG 2 cut(s) 74, 250
BtgZI GCGATG 1 cut(s) 381
BtsCI GGATG 2 cut(s) 227, 475
BtsIMutI CAGTG 1 cut(s) 436
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 240, 364, 690
CfoI GCGC 4 cut(s) 86, 155, 244, 688
Cfr13I GGNCC 2 cut(s) 187, 247
CseI GACGC 2 cut(s) 25, 49
Csp6I GTAC 3 cut(s) 31, 265, 490
CviAII CATG 2 cut(s) 516, 590
CviJI RGCY 7 cut(s) 106, 170, 200, 238, 362, 411, 585
CviKI_1 RGCY 7 cut(s) 106, 170, 200, 238, 362, 411, 585
CviQI GTAC 3 cut(s) 31, 265, 490
DdeI CTNAG 3 cut(s) 138, 599, 643
DinI GGCGCC 1 cut(s) 154
DpnI GATC 3 cut(s) 101, 128, 551
DpnII GATC 3 cut(s) 99, 126, 549
DraIII CACNNNGTG 2 cut(s) 141, 394
Ecl136II GAGCTC 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 527
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 187, 247
Eco53kI GAGCTC 1 cut(s) 106
Eco91I GGTNACC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 106
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoO65I GGTNACC 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 195, 485
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 108
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaeI CATG 2 cut(s) 519, 593
FaiI YATR 8 cut(s) 66, 209, 222, 452, 482, 517, 535, 591
FaqI GGGAC 1 cut(s) 28
FatI CATG 2 cut(s) 515, 589
FblI GTMKAC 2 cut(s) 5, 481
Fnu4HI GCNGC 2 cut(s) 20, 693
FokI GGATG 2 cut(s) 214, 482
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 2 cut(s) 20, 693
FspI TGCGCA 1 cut(s) 243
GlaI GCGC 4 cut(s) 85, 154, 243, 687
GluI GCNGC 2 cut(s) 20, 693
HaeII RGCGCY 2 cut(s) 156, 689
HaeIII GGCC 2 cut(s) 170, 200
HapII CCGG 3 cut(s) 28, 311, 324
HgaI GACGC 2 cut(s) 25, 49
HhaI GCGC 4 cut(s) 86, 155, 244, 688
Hin1I GRCGYC 3 cut(s) 17, 41, 153
Hin1II CATG 2 cut(s) 519, 593
Hin6I GCGC 4 cut(s) 84, 153, 242, 686
HinP1I GCGC 4 cut(s) 84, 153, 242, 686
HincII GTYRAC 2 cut(s) 6, 133
HindII GTYRAC 2 cut(s) 6, 133
HinfI GANTC 2 cut(s) 421, 656
HpaII CCGG 3 cut(s) 28, 311, 324
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 5 cut(s) 6, 33, 133, 265, 482
Hpy188I TCNGA 2 cut(s) 187, 235
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 5 cut(s) 6, 33, 133, 265, 482
Hpy99I CGWCG 3 cut(s) 49, 179, 512
HpyAV CCTTC 3 cut(s) 43, 181, 616
HpyCH4III ACNGT 2 cut(s) 262, 566
HpyCH4IV ACGT 3 cut(s) 507, 526, 680
HpyCH4V TGCA 4 cut(s) 111, 416, 464, 519
HpyF10VI GCNNNNNNNGC 8 cut(s) 159, 372, 443, 452, 461, 639, 683, 692
HpyF3I CTNAG 3 cut(s) 138, 599, 643
HpySE526I ACGT 3 cut(s) 507, 526, 680
Hsp92I GRCGYC 3 cut(s) 17, 41, 153
Hsp92II CATG 2 cut(s) 519, 593
HspAI GCGC 4 cut(s) 84, 153, 242, 686
KasI GGCGCC 1 cut(s) 152
Kzo9I GATC 3 cut(s) 99, 126, 549
LmnI GCTCC 4 cut(s) 60, 235, 547, 598
LweI GCATC 3 cut(s) 424, 455, 680
MaeII ACGT 3 cut(s) 507, 526, 680
MaeIII GTNAC 3 cut(s) 340, 439, 566
MalI GATC 3 cut(s) 101, 128, 551
MbiI CCGCTC 2 cut(s) 542, 598
MboI GATC 3 cut(s) 99, 126, 549
MboII GAAGA 1 cut(s) 486
MhlI GDGCHC 3 cut(s) 57, 108, 324
MluCI AATT 2 cut(s) 179, 291
Mly113I GGCGCC 1 cut(s) 153
MnlI CCTC 7 cut(s) 71, 80, 82, 85, 241, 343, 600
Mph1103I ATGCAT 1 cut(s) 466
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 3 cut(s) 28, 311, 324
MspR9I CCNGG 4 cut(s) 197, 312, 324, 487
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 2 cut(s) 197, 487
MwoI GCNNNNNNNGC 8 cut(s) 159, 372, 443, 452, 461, 639, 683, 692
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 2 cut(s) 312, 324
NdeII GATC 3 cut(s) 99, 126, 549
NlaIII CATG 2 cut(s) 519, 593
NlaIV GGNNCC 2 cut(s) 154, 237
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 439, 566
NsbI TGCGCA 1 cut(s) 243
NsiI ATGCAT 1 cut(s) 466
NspV TTCGAA 1 cut(s) 620
PceI AGGCCT 1 cut(s) 170
PcsI WCGNNNNNNNCGW 2 cut(s) 41, 126
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 2 cut(s) 421, 656
PflFI GACNNNGTC 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 2 cut(s) 21, 694
PluTI GGCGCC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 527
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 195, 485
PspEI GGTNACC 1 cut(s) 340
PspGI CCWGG 2 cut(s) 195, 485
PspN4I GGNNCC 2 cut(s) 154, 237
PspPI GGNCC 2 cut(s) 187, 247
PsyI GACNNNGTC 1 cut(s) 43
RsaI GTAC 3 cut(s) 32, 266, 491
RsaNI GTAC 3 cut(s) 31, 265, 490
RseI CAYNNNNRTG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 108
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 2 cut(s) 20, 693
Sau3AI GATC 3 cut(s) 99, 126, 549
Sau96I GGNCC 2 cut(s) 187, 247
ScrFI CCNGG 4 cut(s) 197, 312, 324, 487
SduI GDGCHC 3 cut(s) 57, 108, 324
SfaNI GCATC 3 cut(s) 424, 455, 680
SfoI GGCGCC 1 cut(s) 154
SfuI TTCGAA 1 cut(s) 620
SinI GGWCC 2 cut(s) 187, 247
SmiMI CAYNNNNRTG 1 cut(s) 206
SnaBI TACGTA 1 cut(s) 527
Sse9I AATT 2 cut(s) 179, 291
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 4 cut(s) 20, 444, 540, 596
SspDI GGCGCC 1 cut(s) 152
SstI GAGCTC 1 cut(s) 108
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 4 cut(s) 195, 310, 322, 485
TaaI ACNGT 2 cut(s) 262, 566
TaiI ACGT 3 cut(s) 510, 529, 683
TaqI TCGA 6 cut(s) 5, 47, 102, 177, 620, 665
TasI AATT 2 cut(s) 179, 291
TatI WGTACW 1 cut(s) 264
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 2 cut(s) 421, 656
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 2 cut(s) 439, 566
TseI GCWGC 1 cut(s) 692
Tsp45I GTSAC 2 cut(s) 439, 566
TspDTI ATGAA 3 cut(s) 216, 504, 522
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 1 cut(s) 436
Tth111I GACNNNGTC 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 187, 247
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 1 cut(s) 179
XmiI GTMKAC 2 cut(s) 5, 481
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.