MD00G1220500.v1.1

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007697
Physical Location & Seq
Forward (+)
51986283 .. 51986852
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1220500.v1.1.491

Sequence Viewer

Length: 570 bp
ATGAATTACCTGAGGTACGCGTGCGAGTACAAGGACACGCTCGCTCGAATTGGTTTTTTCAGAGAGCACTCGAAGATCGAACGCGCCGATTCGACGAGTCGGCCCGAACGCGACGAATACGAGGCATGGGAGGGATCCGGGAACGCGAGCGACGACCAATCGCCGTTTTACTTGCAGCTGATGCGCGAGATGGACTTGATGGACTCGAATCTCGAGACAAAAGCGAAGCTGTACAAGGACGTGGCTTTGAGCTCCATCTTCATGATGAACAACGGGCGATACATCTTGCAGAAAATCAAAGGGTCGGCAGAGATCAACTCGTGTATAGGCGAGACGTGGTACCCTCGGCGGTCGTCGGATCTGAGGCAGTACCACAAGAACTACCAGAGAGAGACGTGGGACCGGCTGCTGCAGTGCTTGAGCCACGAAGGGCTGAGCGTGAACGGGAAGGTGGCGAAGCCGGTGTTGAATGAGAGGTTCAAGAGCTTCAACGCCTTGTTCGATGAGATCCACAAGACGCAAAGCACGTGGGTGGTGAGCGACGAGCATATGCAGTCGGAGGTCGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

190

Amino Acids

22.19

Weight (kDa)

5.99

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 1 - 188 1e-51 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 339
AccII CGCG 5 cut(s) 20, 84, 111, 146, 186
AciI CCGC 1 cut(s) 349
AclWI GGATC 4 cut(s) 129, 142, 366, 502
AcvI CACGTG 1 cut(s) 528
AfaI GTAC 5 cut(s) 17, 29, 233, 341, 371
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 3 cut(s) 469, 481, 490
AjiI CACGTC 3 cut(s) 241, 336, 396
AleI CACNNNNGTG 1 cut(s) 530
AluBI AGCT 4 cut(s) 178, 229, 252, 486
AluI AGCT 4 cut(s) 178, 229, 252, 486
Alw21I GWGCWC 2 cut(s) 69, 254
Alw26I GTCTC 3 cut(s) 209, 326, 386
AlwI GGATC 4 cut(s) 129, 142, 366, 502
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 3 cut(s) 175, 406, 409
Asp718I GGTACC 1 cut(s) 339
AspLEI GCGC 2 cut(s) 86, 186
AspS9I GGNCC 2 cut(s) 102, 400
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 1 cut(s) 547
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 400
AxyI CCTNAGG 1 cut(s) 11
BamHI GGATCC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 339
BanII GRGCYC 1 cut(s) 254
BauI CACGAG 1 cut(s) 319
BbrPI CACGTG 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 69, 254
BbvI GCAGC 3 cut(s) 187, 393, 396
BccI CCATC 3 cut(s) 184, 193, 263
BceAI ACGGC 1 cut(s) 148
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 3 cut(s) 209, 326, 386
BfmI CTRYAG 1 cut(s) 410
BisI GCNGC 3 cut(s) 176, 407, 410
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 3 cut(s) 177, 408, 411
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 212
BmgBI CACGTC 3 cut(s) 241, 336, 396
BmgT120I GGNCC 2 cut(s) 102, 400
BmiI GGNNCC 3 cut(s) 136, 341, 401
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 302, 334
Bpu1102I GCTNAGC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 139
BsaAI YACGTR 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 344
BsaXI ACNNNNNCTCC 1 cut(s) 551
Bse118I RCCGGY 2 cut(s) 402, 460
Bse21I CCTNAGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 344
BseMII CTCAG 2 cut(s) 353, 425
BseXI GCAGC 3 cut(s) 187, 393, 396
Bsh1236I CGCG 5 cut(s) 20, 84, 111, 146, 186
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 339
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 2 cut(s) 69, 254
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 3 cut(s) 138, 403, 461
BslFI GGGAC 1 cut(s) 413
BsmAI GTCTC 3 cut(s) 209, 326, 386
BsmBI CGTCTC 2 cut(s) 326, 386
BsmFI GGGAC 1 cut(s) 413
BsnI GGCC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 69, 254
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 5 cut(s) 75, 134, 312, 358, 507
Bsp1720I GCTNAGC 1 cut(s) 434
BspACI CCGC 1 cut(s) 349
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 2 cut(s) 354, 426
BspFNI CGCG 5 cut(s) 20, 84, 111, 146, 186
BspHI TCATGA 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 136, 341, 401
BspMAI CTGCAG 1 cut(s) 414
BspPI GGATC 4 cut(s) 129, 142, 366, 502
BspT107I GGYRCC 1 cut(s) 339
BsrFI RCCGGY 2 cut(s) 402, 460
BsrGI TGTACA 1 cut(s) 231
BssAI RCCGGY 2 cut(s) 402, 460
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 5 cut(s) 75, 134, 312, 358, 507
BssSI CACGAG 1 cut(s) 319
Bst2BI CACGAG 1 cut(s) 319
BstAPI GCANNNNNTGC 1 cut(s) 181
BstAUI TGTACA 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 528
BstC8I GCNNGC 3 cut(s) 22, 42, 148
BstDEI CTNAG 3 cut(s) 11, 362, 434
BstFNI CGCG 5 cut(s) 20, 84, 111, 146, 186
BstHHI GCGC 2 cut(s) 86, 186
BstKTI GATC 5 cut(s) 78, 137, 315, 361, 510
BstMAI GTCTC 3 cut(s) 209, 326, 386
BstMBI GATC 5 cut(s) 75, 134, 312, 358, 507
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstSCI CCNGG 1 cut(s) 137
BstSFI CTRYAG 1 cut(s) 410
BstUI CGCG 5 cut(s) 20, 84, 111, 146, 186
BstV1I GCAGC 3 cut(s) 187, 393, 396
BstX2I RGATCY 3 cut(s) 134, 358, 507
BstYI RGATCY 3 cut(s) 134, 358, 507
Bsu36I CCTNAGG 1 cut(s) 11
BsuRI GGCC 1 cut(s) 103
BtrI CACGTC 3 cut(s) 241, 336, 396
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 3 cut(s) 22, 42, 148
CciI TCATGA 1 cut(s) 261
CfoI GCGC 2 cut(s) 86, 186
Cfr10I RCCGGY 2 cut(s) 402, 460
Cfr13I GGNCC 2 cut(s) 102, 400
CseI GACGC 1 cut(s) 526
Csp6I GTAC 5 cut(s) 16, 28, 232, 340, 370
CspCI CAANNNNNGTGG 2 cut(s) 509, 544
CviAII CATG 2 cut(s) 126, 262
CviQI GTAC 5 cut(s) 16, 28, 232, 340, 370
DdeI CTNAG 3 cut(s) 11, 362, 434
DpnI GATC 5 cut(s) 77, 136, 314, 360, 509
DpnII GATC 5 cut(s) 75, 134, 312, 358, 507
Ecl136II GAGCTC 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 252
Eco72I CACGTG 1 cut(s) 528
Eco81I CCTNAGG 1 cut(s) 11
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 254
Esp3I CGTCTC 2 cut(s) 326, 386
FaeI CATG 2 cut(s) 129, 265
FaiI YATR 5 cut(s) 127, 263, 326, 549, 551
FaqI GGGAC 1 cut(s) 413
FatI CATG 2 cut(s) 125, 261
FauNDI CATATG 1 cut(s) 549
Fnu4HI GCNGC 3 cut(s) 176, 407, 410
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 3 cut(s) 176, 407, 410
GlaI GCGC 2 cut(s) 85, 185
GluI GCNGC 3 cut(s) 176, 407, 410
HaeIII GGCC 1 cut(s) 103
HapII CCGG 3 cut(s) 138, 403, 461
HgaI GACGC 1 cut(s) 526
HhaI GCGC 2 cut(s) 86, 186
Hin1II CATG 2 cut(s) 129, 265
Hin6I GCGC 2 cut(s) 84, 184
HinP1I GCGC 2 cut(s) 84, 184
HinfI GANTC 4 cut(s) 89, 97, 203, 208
HpaII CCGG 3 cut(s) 138, 403, 461
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 4 cut(s) 62, 358, 363, 559
Hpy188III TCNNGA 4 cut(s) 212, 214, 262, 481
Hpy8I GTNNAC 1 cut(s) 442
Hpy99I CGWCG 5 cut(s) 97, 116, 155, 358, 545
HpyAV CCTTC 2 cut(s) 422, 442
HpyCH4IV ACGT 4 cut(s) 240, 335, 395, 527
HpyCH4V TGCA 4 cut(s) 175, 289, 412, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 3 cut(s) 11, 362, 434
HpySE526I ACGT 4 cut(s) 240, 335, 395, 527
Hsp92II CATG 2 cut(s) 129, 265
HspAI GCGC 2 cut(s) 84, 184
KpnI GGTACC 1 cut(s) 343
Kzo9I GATC 5 cut(s) 75, 134, 312, 358, 507
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 5 cut(s) 23, 151, 398, 416, 474
Lsp1109I GCAGC 3 cut(s) 187, 393, 396
LweI GCATC 1 cut(s) 171
MaeII ACGT 4 cut(s) 240, 335, 395, 527
MalI GATC 5 cut(s) 77, 136, 314, 360, 509
MboI GATC 5 cut(s) 75, 134, 312, 358, 507
MboII GAAGA 2 cut(s) 85, 250
MflI RGATCY 3 cut(s) 134, 358, 507
MhlI GDGCHC 2 cut(s) 69, 254
MluCI AATT 2 cut(s) 4, 48
MluI ACGCGT 1 cut(s) 18
MlyI GAGTC 2 cut(s) 106, 197
MmeI TCCRAC 2 cut(s) 336, 537
MnlI CCTC 7 cut(s) 6, 115, 124, 354, 357, 468, 553
MslI CAYNNNNRTG 2 cut(s) 260, 530
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 3 cut(s) 138, 403, 461
MspR9I CCNGG 1 cut(s) 139
MvnI CGCG 5 cut(s) 20, 84, 111, 146, 186
MwoI GCNNNNNNNGC 1 cut(s) 181
NciI CCSGG 1 cut(s) 139
NdeI CATATG 1 cut(s) 549
NdeII GATC 5 cut(s) 75, 134, 312, 358, 507
NlaIII CATG 2 cut(s) 129, 265
NlaIV GGNNCC 3 cut(s) 136, 341, 401
NmeAIII GCCGAG 1 cut(s) 325
OliI CACNNNNGTG 1 cut(s) 530
PaeR7I CTCGAG 1 cut(s) 212
PagI TCATGA 1 cut(s) 261
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 150, 498, 524
PfeI GAWTC 2 cut(s) 89, 208
PfoI TCCNGGA 1 cut(s) 137
PkrI GCNGC 3 cut(s) 177, 408, 411
PleI GAGTC 2 cut(s) 105, 197
PmaCI CACGTG 1 cut(s) 528
PmlI CACGTG 1 cut(s) 528
PpsI GAGTC 2 cut(s) 105, 197
Ppu21I YACGTR 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 254
PspCI CACGTG 1 cut(s) 528
PspN4I GGNNCC 3 cut(s) 136, 341, 401
PspPI GGNCC 2 cut(s) 102, 400
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 3 cut(s) 134, 358, 507
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 5 cut(s) 17, 29, 233, 341, 371
RsaNI GTAC 5 cut(s) 16, 28, 232, 340, 370
RseI CAYNNNNRTG 2 cut(s) 260, 530
SacI GAGCTC 1 cut(s) 254
SatI GCNGC 3 cut(s) 176, 407, 410
Sau3AI GATC 5 cut(s) 75, 134, 312, 358, 507
Sau96I GGNCC 2 cut(s) 102, 400
SchI GAGTC 2 cut(s) 106, 197
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 2 cut(s) 69, 254
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 1 cut(s) 410
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 400
SlaI CTCGAG 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 260, 530
SmlI CTYRAG 2 cut(s) 212, 418
SmoI CTYRAG 2 cut(s) 212, 418
Sse9I AATT 2 cut(s) 4, 48
SsiI CCGC 1 cut(s) 349
SstI GAGCTC 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 137
TaiI ACGT 4 cut(s) 243, 338, 398, 530
TaqI TCGA 7 cut(s) 46, 71, 78, 92, 206, 213, 501
TasI AATT 2 cut(s) 4, 48
TatI WGTACW 2 cut(s) 27, 231
TfiI GAWTC 2 cut(s) 89, 208
TscAI CASTG 1 cut(s) 419
TseI GCWGC 3 cut(s) 175, 406, 409
TspDTI ATGAA 3 cut(s) 17, 250, 281
TspRI CASTG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 400
XhoI CTCGAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.