MD17G1010900.v1.1

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007697
Physical Location & Seq
Forward (+)
674265 .. 676712
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1010900.v1.1.491

Sequence Viewer

Length: 2448 bp
ATGGAGAAGAACCTTCCGGACAAAGACGATCAGCCCAAACCCGACGCACCGCCACCGGACTCCTCCGCCGCCGCTACAATTCCAAAGCCTAATGACGATCCCAAGTCCACCACCACCACTGCTACTGCTACTACAAGTCCTGACGGCGATAATAAGTCCGCTGGCACTAAGCCAGACGATGACCAAAAGCCGTCTAATCCAACGGCTGAGGTTCAGGATGAGCAGAACTCCATTGCAAAGCTTGATGATCCAAATGTAAATAACGCCGAGACCAAAGTTTATCAAGGGAAGGAAGGTGATGACCCGGATTACGAGATAAAAAAGTTCAGCAGTATGAGGGTCAATAGTACCAGAGTGAAGGATTTTAAAGCTGAGAATGTTGAAGATCAGAACGATCCACTTCCGGTACTGGATGAAGATCCAGTTCCCGAACCCGAACCCGAACCCGAACCTGAACCTGAACCTGAACCGGAAGAGGAACCTAGCCTCGACAAGGTCTCGGAGGATCTCGACCACTGCATCTCGTCTCTGCCTGAAACCGAAAAACTTGAAAACGACGTCGTGGACGCCCTCAAGTTTCCGATTGAGCTTCCGAAGCTGGTGGGAAAGTTTTTGAATCTAGTTGAGGACGAGGTGGTGGCGAAACGCGACTCTGGCGACGCTAAGGTGAAATGGTGTCAGGAGCCTGAGAAGGACGCGTTGTTTCTGGATGCTCTTGATCGGATAGGGAGGCTGAGCAAGTACCTCTCTTTCCTCGGATTGAAATTCGAAGGGAGTCACGGCGCGTTGATCAACCACCTCGGAAGCATTCAGCACCGAGCGTTGCTGTATCTGGAAGAAGAATTCAGAATTCTTCTAGAAGAATCTAGAACCTGCACCGTCACTGACTCTGCATCCGGCGACCATAAGGACAATAAAGATCATAAGGACAGCAAAGAGCACAGTACTAAAGGCGGGAATAATAACAGCGGCAGTAATAAGCAACAGGATCATCAAGATCAGGAAAGCAGCGGCGGCGCATTGCCGGATCAGGCGGAGTCGGGAGGCGGCGGAGATGAATTACAGGCCGATCAGTTCCCTGGATATGCTCAAGAGGTAGCGAACAATCTGAACAAGATAGCCAAAAAGATGATATCCAGCGGATACGAAACGGAGTGTTGCGAGGTTTACATGATCTCGAGAAGGCACTTGTTCGATGAGACTCTGCACAAGTTGGGACTAGAGAAGCACAGCCTCGACGACATTCAGAAAATGCACTGGGAATCGCTCGAAAGAGAGATCGTGTCATGGGTGAAGGCCTTCAAGGAATGCACCACCGTATACTTCTCCGGCGAGCGGAAGTTCGTGGAGGCGGTGTTTGCTGACTACCCGTCCATCTCGTCGAGCCTGTTTAGCAATTTGACCAAGGGGGTGATGATACAGCTTCTCAATTTCGCGGAGGGGATTGCGATATCGAAGCGGTCGGCGGAGAAGCTGTTTAAGACGCTGGACATGTACGAGGCGTTGCGGGAGGTGATCCCCAAAATGGACAACTTGTTTCCGGCAGACTGCGTGAACGAGCTGAAGCACGAGACCTCTAATGTCCGGACCCGCCTCGGCGTGGCGGCGATATGCATTTTCTGTGATCTGGAGAACTCAATTAAGGCTGAGACGGGTAGAAACCCGGTTCCGGGAGGGGCGGTCCATCCGCTGACCCGATACACGATGAATTACCTGAGGTACGCGTGCGAGTACAAGGACACGCTCGAATTGGTTTTCAGAGAGCACTCGAAGATCGAACGCGCCGATTCGACGAGTCGGCCCGAACGCGGCGAGTACGAGGCAGGGGAGGGATCCGGGAACGCGAGTGACGACCAATCGCCGTTTTACTTGCAGCTGATGCGCGTGATGGACTTGCTGGACTCGAATCTCGAGACGAAAGCGAAGCTGTACAAGGACGTGGCTCTGAGCTCCATCTTCATGATGAACAACGGGCGATACATCTTGCAGAAAATCAAAGGGTCGGCAGAGATCAACTCGTGTATAGGCGAGACGTGGTACCGTCGGCGGTCGTCGGATCTGAGGCAGTACCACAAGAACTACCAGAGGGAGACGTGGGGCCGGCTGCTGCAGTGCCTGAGCCACGAAGGGCTGAGCGTGAACGGGAAGGTGGCGAAGCCGGTGTTGAAGGAGAGGTTCAAGAGCTTCAACGCCTTGTTCGATGAGATCCACAAGACGCAAAGCACGTGGGTGGTGAGCGACGAGCAGATGCAGTCGGAGTTGAGGGTGTCGATATCAGCGGTGGTGATTCCGGCGTACCGATCATTCTTGGGGCGGTTCTCGCAGGTTTTCGATGCGGGGAGGCAGACGGAGAAGTACGTAAAATTCCAGCCGGAAGATATAGAGACGTACATTGAGGAGCTATTTGATGGAAACCCCAACTCCTCCATATTCAAAAAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

816

Amino Acids

91.77

Weight (kDa)

4.95

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 429 - 800 1e-123 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 561
Acc36I ACCTGC 2 cut(s) 881, 2314
Acc65I GGTACC 1 cut(s) 2037
AccB1I GGYRCC 1 cut(s) 2037
AccBSI CCGCTC 1 cut(s) 1336
AccI GTMKAC 1 cut(s) 1320
AccII CGCG 9 cut(s) 648, 698, 785, 1436, 1724, 1782, 1809, 1844, 1884
AccIII TCCGGA 2 cut(s) 16, 1584
AcsI RAATTY 4 cut(s) 764, 842, 849, 2363
AcuI CTGAAG 1 cut(s) 1583
AcvI CACGTG 1 cut(s) 2226
AcyI GRCGYC 2 cut(s) 558, 567
AfiI CCNNNNNNNGG 7 cut(s) 493, 1335, 1525, 1600, 1669, 1670, 1808
AflIII ACRYGT 3 cut(s) 696, 1491, 1722
AgsI TTSAA 9 cut(s) 383, 551, 616, 763, 1303, 2167, 2179, 2188, 2434
AhdI GACNNNNNGTC 1 cut(s) 1369
AjiI CACGTC 3 cut(s) 1939, 2034, 2094
AjnI CCWGG 1 cut(s) 1078
AleI CACNNNNGTG 1 cut(s) 2228
Alw21I GWGCWC 3 cut(s) 942, 1767, 1952
AlwNI CAGNNNCTG 1 cut(s) 2116
Ama87I CYCGRG 2 cut(s) 1177, 1910
Aor13HI TCCGGA 2 cut(s) 16, 1584
AoxI GGCC 4 cut(s) 1065, 1296, 1799, 2098
ApeKI GCWGC 4 cut(s) 1008, 1873, 2104, 2107
ApoI RAATTY 4 cut(s) 764, 842, 849, 2363
Asp700I GAANNNNTTC 1 cut(s) 1298
Asp718I GGTACC 1 cut(s) 2037
AspLEI GCGC 4 cut(s) 785, 1019, 1784, 1884
AspS9I GGNCC 4 cut(s) 1587, 1681, 1800, 2098
AsuC2I CCSGG 4 cut(s) 305, 1664, 1671, 1837
AsuHPI GGTGA 7 cut(s) 308, 679, 1303, 1423, 1525, 2245, 2296
AsuII TTCGAA 1 cut(s) 768
AvaI CYCGRG 2 cut(s) 1177, 1910
AvaII GGWCC 2 cut(s) 1587, 1681
AxyI CCTNAGG 1 cut(s) 1715
BamHI GGATCC 1 cut(s) 1832
BanI GGYRCC 1 cut(s) 2037
BanII GRGCYC 1 cut(s) 1952
BauI CACGAG 2 cut(s) 1568, 2017
BbrPI CACGTG 1 cut(s) 2226
Bbv12I GWGCWC 3 cut(s) 942, 1767, 1952
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 1020, 1885, 2091, 2094
BccI CCATC 5 cut(s) 1382, 1692, 1882, 1961, 2402
BceAI ACGGC 5 cut(s) 160, 175, 219, 796, 1846
BciT130I CCWGG 1 cut(s) 1080
BciVI GTATCC 1 cut(s) 1136
BclI TGATCA 1 cut(s) 789
BcnI CCSGG 4 cut(s) 305, 1664, 1671, 1837
BfaI CTAG 5 cut(s) 483, 620, 857, 867, 1220
BfmI CTRYAG 1 cut(s) 2108
BfuAI ACCTGC 2 cut(s) 881, 2314
BfuI GTATCC 1 cut(s) 1136
BlpI GCTNAGC 2 cut(s) 734, 2132
BmcAI AGTACT 1 cut(s) 946
Bme1390I CCNGG 5 cut(s) 305, 1080, 1664, 1671, 1837
Bme18I GGWCC 2 cut(s) 1587, 1681
BmeRI GACNNNNNGTC 1 cut(s) 1369
BmeT110I CYCGRG 2 cut(s) 1177, 1910
BmgBI CACGTC 3 cut(s) 1939, 2034, 2094
BmgT120I GGNCC 4 cut(s) 1587, 1681, 1800, 2098
BmiI GGNNCC 7 cut(s) 480, 684, 1589, 1668, 1834, 2039, 2099
BmrFI CCNGG 5 cut(s) 305, 1080, 1664, 1671, 1837
BmrI ACTGGG 1 cut(s) 1267
BmsI GCATC 6 cut(s) 528, 700, 902, 1869, 2238, 2323
BmuI ACTGGG 1 cut(s) 1267
BplI GAGNNNNNCTC 4 cut(s) 212, 244, 2000, 2032
BpmI CTGGAG 1 cut(s) 1649
Bpu10I CCTNAGC 3 cut(s) 207, 663, 2117
Bpu1102I GCTNAGC 2 cut(s) 734, 2132
Bpu14I TTCGAA 1 cut(s) 768
BpuEI CTTGAG 2 cut(s) 557, 1074
BpuMI CCSGG 4 cut(s) 305, 1664, 1671, 1837
BsaAI YACGTR 2 cut(s) 2226, 2359
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 2 cut(s) 558, 567
BsaI GGTCTC 3 cut(s) 263, 502, 1565
BsaJI CCNNGG 5 cut(s) 754, 799, 1078, 1404, 1594
BsaWI WCCGGW 5 cut(s) 16, 55, 403, 469, 1584
BsaXI ACNNNNNCTCC 8 cut(s) 1340, 1370, 1460, 1490, 1665, 1695, 2405, 2435
Bsc4I CCNNNNNNNGG 7 cut(s) 493, 1335, 1525, 1600, 1669, 1670, 1808
Bse118I RCCGGY 2 cut(s) 2100, 2158
Bse1I ACTGG 3 cut(s) 414, 422, 1262
Bse21I CCTNAGG 1 cut(s) 1715
Bse3DI GCAATG 2 cut(s) 231, 1019
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 2 cut(s) 16, 1584
BseBI CCWGG 1 cut(s) 1080
BseDI CCNNGG 5 cut(s) 754, 799, 1078, 1404, 1594
BseGI GGATG 5 cut(s) 223, 418, 715, 893, 1684
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 7 cut(s) 493, 1335, 1525, 1600, 1669, 1670, 1808
BseMI GCAATG 2 cut(s) 231, 1019
BseNI ACTGG 3 cut(s) 414, 422, 1262
BseRI GAGGAG 3 cut(s) 52, 2411, 2413
BseXI GCAGC 4 cut(s) 1020, 1885, 2091, 2094
BsgI GTGCAG 2 cut(s) 859, 1190
Bsh1236I CGCG 9 cut(s) 648, 698, 785, 1436, 1724, 1782, 1809, 1844, 1884
Bsh1285I CGRYCG 2 cut(s) 1463, 2051
BshFI GGCC 4 cut(s) 1067, 1298, 1801, 2100
BshNI GGYRCC 1 cut(s) 2037
BsiEI CGRYCG 2 cut(s) 1463, 2051
BsiHKAI GWGCWC 3 cut(s) 942, 1767, 1952
BsiHKCI CYCGRG 2 cut(s) 1177, 1910
BslFI GGGAC 1 cut(s) 1230
BslI CCNNNNNNNGG 7 cut(s) 493, 1335, 1525, 1600, 1669, 1670, 1808
BsmBI CGTCTC 6 cut(s) 531, 1643, 1907, 2024, 2084, 2378
BsmFI GGGAC 1 cut(s) 1230
BsmI GAATGC 2 cut(s) 807, 1313
BsnI GGCC 4 cut(s) 1067, 1298, 1801, 2100
Bso31I GGTCTC 3 cut(s) 263, 502, 1565
BsoBI CYCGRG 2 cut(s) 1177, 1910
Bsp119I TTCGAA 1 cut(s) 768
Bsp1286I GDGCHC 3 cut(s) 942, 1767, 1952
Bsp13I TCCGGA 2 cut(s) 16, 1584
Bsp1407I TGTACA 1 cut(s) 1929
Bsp1720I GCTNAGC 2 cut(s) 734, 2132
BspANI GGCC 4 cut(s) 1067, 1298, 1801, 2100
BspEI TCCGGA 2 cut(s) 16, 1584
BspFNI CGCG 9 cut(s) 648, 698, 785, 1436, 1724, 1782, 1809, 1844, 1884
BspHI TCATGA 1 cut(s) 1959
BspLI GGNNCC 7 cut(s) 480, 684, 1589, 1668, 1834, 2039, 2099
BspMAI CTGCAG 1 cut(s) 2112
BspMI ACCTGC 2 cut(s) 881, 2314
BspT104I TTCGAA 1 cut(s) 768
BspT107I GGYRCC 1 cut(s) 2037
BspTNI GGTCTC 3 cut(s) 263, 502, 1565
BsrBI CCGCTC 1 cut(s) 1336
BsrDI GCAATG 2 cut(s) 231, 1019
BsrFI RCCGGY 2 cut(s) 2100, 2158
BsrGI TGTACA 1 cut(s) 1929
BsrI ACTGG 3 cut(s) 414, 422, 1262
BssAI RCCGGY 2 cut(s) 2100, 2158
BssECI CCNNGG 5 cut(s) 754, 799, 1078, 1404, 1594
BssNAI GTATAC 1 cut(s) 1321
BssNI GRCGYC 2 cut(s) 558, 567
BssSI CACGAG 2 cut(s) 1568, 2017
BssT1I CCWWGG 1 cut(s) 1404
Bst1107I GTATAC 1 cut(s) 1321
Bst2BI CACGAG 2 cut(s) 1568, 2017
Bst2UI CCWGG 1 cut(s) 1080
Bst4CI ACNGT 4 cut(s) 880, 944, 1318, 2042
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 2 cut(s) 558, 567
BstAPI GCANNNNNTGC 1 cut(s) 1879
BstAUI TGTACA 1 cut(s) 1929
BstBAI YACGTR 2 cut(s) 2226, 2359
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 4 cut(s) 163, 1334, 1726, 2102
BstENI CCTNNNNNAGG 1 cut(s) 491
BstF5I GGATG 5 cut(s) 223, 418, 715, 893, 1684
BstFNI CGCG 9 cut(s) 648, 698, 785, 1436, 1724, 1782, 1809, 1844, 1884
BstHHI GCGC 4 cut(s) 785, 1019, 1784, 1884
BstMCI CGRYCG 2 cut(s) 1463, 2051
BstMWI GCNNNNNNNGC 7 cut(s) 595, 654, 1014, 1358, 1392, 1879, 2320
BstNI CCWGG 1 cut(s) 1080
BstNSI RCATGY 1 cut(s) 1495
BstSCI CCNGG 5 cut(s) 303, 1078, 1662, 1669, 1835
BstSFI CTRYAG 1 cut(s) 2108
BstSNI TACGTA 1 cut(s) 2359
BstUI CGCG 9 cut(s) 648, 698, 785, 1436, 1724, 1782, 1809, 1844, 1884
BstV1I GCAGC 4 cut(s) 1020, 1885, 2091, 2094
BstX2I RGATCY 5 cut(s) 418, 505, 1832, 2056, 2205
BstYI RGATCY 5 cut(s) 418, 505, 1832, 2056, 2205
BstZ17I GTATAC 1 cut(s) 1321
Bsu36I CCTNAGG 1 cut(s) 1715
BsuI GTATCC 1 cut(s) 1136
BsuRI GGCC 4 cut(s) 1067, 1298, 1801, 2100
BtrI CACGTC 3 cut(s) 1939, 2034, 2094
BtsCI GGATG 5 cut(s) 223, 418, 715, 893, 1684
BtsI GCAGTG 3 cut(s) 117, 514, 2117
BtsIMutI CAGTG 5 cut(s) 117, 514, 882, 1255, 2117
BveI ACCTGC 2 cut(s) 881, 2314
Cac8I GCNNGC 4 cut(s) 163, 1334, 1726, 2102
CaiI CAGNNNCTG 1 cut(s) 2116
CciI TCATGA 1 cut(s) 1959
CfoI GCGC 4 cut(s) 785, 1019, 1784, 1884
Cfr10I RCCGGY 2 cut(s) 2100, 2158
Cfr13I GGNCC 4 cut(s) 1587, 1681, 1800, 2098
CseI GACGC 6 cut(s) 53, 575, 668, 704, 1492, 2224
CspCI CAANNNNNGTGG 2 cut(s) 2207, 2242
CviAII CATG 4 cut(s) 1171, 1287, 1492, 1960
DraI TTTAAA 1 cut(s) 367
DriI GACNNNNNGTC 1 cut(s) 1369
Eam1104I CTCTTC 1 cut(s) 468
Eam1105I GACNNNNNGTC 1 cut(s) 1369
EarI CTCTTC 1 cut(s) 468
EciI GGCGGA 4 cut(s) 55, 1049, 1065, 1481
Ecl136II GAGCTC 1 cut(s) 1950
Eco105I TACGTA 1 cut(s) 2359
Eco130I CCWWGG 1 cut(s) 1404
Eco147I AGGCCT 1 cut(s) 1298
Eco24I GRGCYC 1 cut(s) 1952
Eco31I GGTCTC 3 cut(s) 263, 502, 1565
Eco32I GATATC 3 cut(s) 1134, 1452, 2274
Eco47I GGWCC 2 cut(s) 1587, 1681
Eco53kI GAGCTC 1 cut(s) 1950
Eco57I CTGAAG 1 cut(s) 1583
Eco72I CACGTG 1 cut(s) 2226
Eco81I CCTNAGG 1 cut(s) 1715
Eco88I CYCGRG 2 cut(s) 1177, 1910
EcoICRI GAGCTC 1 cut(s) 1950
EcoNI CCTNNNNNAGG 1 cut(s) 491
EcoRI GAATTC 2 cut(s) 842, 849
EcoRII CCWGG 1 cut(s) 1078
EcoRV GATATC 3 cut(s) 1134, 1452, 2274
EcoT14I CCWWGG 1 cut(s) 1404
EcoT22I ATGCAT 1 cut(s) 1616
EcoT38I GRGCYC 1 cut(s) 1952
ErhI CCWWGG 1 cut(s) 1404
Esp3I CGTCTC 6 cut(s) 531, 1643, 1907, 2024, 2084, 2378
FaeI CATG 4 cut(s) 1174, 1290, 1495, 1963
FaqI GGGAC 1 cut(s) 1230
FatI CATG 4 cut(s) 1170, 1286, 1491, 1959
FauI CCCGC 4 cut(s) 947, 1500, 1598, 2329
FbaI TGATCA 1 cut(s) 789
FblI GTMKAC 1 cut(s) 1320
FokI GGATG 5 cut(s) 230, 425, 722, 880, 1671
FriOI GRGCYC 1 cut(s) 1952
FspBI CTAG 5 cut(s) 483, 620, 857, 867, 1220
GlaI GCGC 4 cut(s) 784, 1018, 1783, 1883
GsuI CTGGAG 1 cut(s) 1649
HaeIII GGCC 4 cut(s) 1067, 1298, 1801, 2100
HgaI GACGC 6 cut(s) 53, 575, 668, 704, 1492, 2224
HhaI GCGC 4 cut(s) 785, 1019, 1784, 1884
Hin1I GRCGYC 2 cut(s) 558, 567
Hin1II CATG 4 cut(s) 1174, 1290, 1495, 1963
Hin6I GCGC 4 cut(s) 783, 1017, 1782, 1882
HinP1I GCGC 4 cut(s) 783, 1017, 1782, 1882
HindIII AAGCTT 1 cut(s) 239
HphI GGTGA 7 cut(s) 308, 679, 1303, 1423, 1525, 2245, 2296
Hpy166II GTNNAC 6 cut(s) 108, 565, 1168, 1321, 1555, 2140
Hpy8I GTNNAC 6 cut(s) 108, 565, 1168, 1321, 1555, 2140
HpyCH4III ACNGT 4 cut(s) 880, 944, 1318, 2042
HpyCH4IV ACGT 7 cut(s) 558, 1938, 2033, 2093, 2225, 2358, 2387
HpyF10VI GCNNNNNNNGC 7 cut(s) 595, 654, 1014, 1358, 1392, 1879, 2320
HpySE526I ACGT 7 cut(s) 558, 1938, 2033, 2093, 2225, 2358, 2387
Hsp92I GRCGYC 2 cut(s) 558, 567
Hsp92II CATG 4 cut(s) 1174, 1290, 1495, 1963
HspAI GCGC 4 cut(s) 783, 1017, 1782, 1882
Kpn2I TCCGGA 2 cut(s) 16, 1584
KpnI GGTACC 1 cut(s) 2041
KroI GCCGGC 1 cut(s) 2100
KroNI GCCGGC 1 cut(s) 2102
Ksp22I TGATCA 1 cut(s) 789
LmnI GCTCC 3 cut(s) 682, 1955, 2398
Lsp1109I GCAGC 4 cut(s) 1020, 1885, 2091, 2094
LweI GCATC 6 cut(s) 528, 700, 902, 1869, 2238, 2323
MaeI CTAG 5 cut(s) 483, 620, 857, 867, 1220
MaeII ACGT 7 cut(s) 558, 1938, 2033, 2093, 2225, 2358, 2387
MaeIII GTNAC 3 cut(s) 776, 880, 1847
MbiI CCGCTC 1 cut(s) 1336
MflI RGATCY 5 cut(s) 418, 505, 1832, 2056, 2205
MhlI GDGCHC 3 cut(s) 942, 1767, 1952
MluI ACGCGT 2 cut(s) 696, 1722
MlyI GAGTC 8 cut(s) 53, 644, 784, 881, 1046, 1195, 1804, 1895
MmeI TCCRAC 3 cut(s) 224, 2034, 2235
Mph1103I ATGCAT 1 cut(s) 1616
MroI TCCGGA 2 cut(s) 16, 1584
MroNI GCCGGC 1 cut(s) 2100
MroXI GAANNNNTTC 1 cut(s) 1298
MseI TTAA 3 cut(s) 366, 1479, 1641
MslI CAYNNNNRTG 2 cut(s) 1958, 2228
MspA1I CMGCKG 7 cut(s) 161, 969, 1011, 1140, 1690, 1876, 2279
MspR9I CCNGG 5 cut(s) 305, 1080, 1664, 1671, 1837
Mva1269I GAATGC 2 cut(s) 807, 1313
MvaI CCWGG 1 cut(s) 1080
MvnI CGCG 9 cut(s) 648, 698, 785, 1436, 1724, 1782, 1809, 1844, 1884
MwoI GCNNNNNNNGC 7 cut(s) 595, 654, 1014, 1358, 1392, 1879, 2320
NaeI GCCGGC 1 cut(s) 2102
NciI CCSGG 4 cut(s) 305, 1664, 1671, 1837
NgoMIV GCCGGC 1 cut(s) 2100
NlaIII CATG 4 cut(s) 1174, 1290, 1495, 1963
NlaIV GGNNCC 7 cut(s) 480, 684, 1589, 1668, 1834, 2039, 2099
NmeAIII GCCGAG 2 cut(s) 292, 1575
NmuCI GTSAC 3 cut(s) 776, 880, 1847
NsiI ATGCAT 1 cut(s) 1616
NspI RCATGY 1 cut(s) 1495
NspV TTCGAA 1 cut(s) 768
OliI CACNNNNGTG 1 cut(s) 2228
PaeR7I CTCGAG 2 cut(s) 1177, 1910
PagI TCATGA 1 cut(s) 1959
PceI AGGCCT 1 cut(s) 1298
PciI ACATGT 1 cut(s) 1491
PcsI WCGNNNNNNNCGW 4 cut(s) 1782, 1848, 2196, 2222
PctI GAATGC 2 cut(s) 807, 1313
PdiI GCCGGC 1 cut(s) 2102
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 6 cut(s) 616, 863, 1262, 1787, 1906, 2287
PflFI GACNNNGTC 1 cut(s) 494
PfoI TCCNGGA 2 cut(s) 1669, 1835
PleI GAGTC 8 cut(s) 53, 644, 783, 881, 1045, 1195, 1803, 1895
PmaCI CACGTG 1 cut(s) 2226
PmlI CACGTG 1 cut(s) 2226
PpsI GAGTC 8 cut(s) 53, 644, 783, 881, 1045, 1195, 1803, 1895
Ppu21I YACGTR 2 cut(s) 2226, 2359
PscI ACATGT 1 cut(s) 1491
Psp124BI GAGCTC 1 cut(s) 1952
Psp6I CCWGG 1 cut(s) 1078
PspCI CACGTG 1 cut(s) 2226
PspGI CCWGG 1 cut(s) 1078
PspN4I GGNNCC 7 cut(s) 480, 684, 1589, 1668, 1834, 2039, 2099
PspPI GGNCC 4 cut(s) 1587, 1681, 1800, 2098
PstI CTGCAG 1 cut(s) 2112
PstNI CAGNNNCTG 1 cut(s) 2116
PsuI RGATCY 5 cut(s) 418, 505, 1832, 2056, 2205
PsyI GACNNNGTC 1 cut(s) 494
PvuII CAGCTG 1 cut(s) 1876
RseI CAYNNNNRTG 2 cut(s) 1958, 2228
SacI GAGCTC 1 cut(s) 1952
SaqAI TTAA 3 cut(s) 366, 1479, 1641
Sau96I GGNCC 4 cut(s) 1587, 1681, 1800, 2098
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 8 cut(s) 53, 644, 784, 881, 1046, 1195, 1804, 1895
ScrFI CCNGG 5 cut(s) 305, 1080, 1664, 1671, 1837
SduI GDGCHC 3 cut(s) 942, 1767, 1952
SfaNI GCATC 6 cut(s) 528, 700, 902, 1869, 2238, 2323
SfcI CTRYAG 1 cut(s) 2108
Sfr274I CTCGAG 2 cut(s) 1177, 1910
SfuI TTCGAA 1 cut(s) 768
SinI GGWCC 2 cut(s) 1587, 1681
SlaI CTCGAG 2 cut(s) 1177, 1910
SmiMI CAYNNNNRTG 2 cut(s) 1958, 2228
SmlI CTYRAG 4 cut(s) 572, 1089, 1177, 1910
SmoI CTYRAG 4 cut(s) 572, 1089, 1177, 1910
SnaBI TACGTA 1 cut(s) 2359
SseBI AGGCCT 1 cut(s) 1298
SspMI CTAG 5 cut(s) 483, 620, 857, 867, 1220
SstI GAGCTC 1 cut(s) 1952
StuI AGGCCT 1 cut(s) 1298
StyD4I CCNGG 5 cut(s) 303, 1078, 1662, 1669, 1835
StyI CCWWGG 1 cut(s) 1404
TaaI ACNGT 4 cut(s) 880, 944, 1318, 2042
TaiI ACGT 7 cut(s) 561, 1941, 2036, 2096, 2228, 2361, 2390
TatI WGTACW 3 cut(s) 944, 1731, 1929
TauI GCSGC 8 cut(s) 71, 74, 972, 1014, 1017, 1050, 1607, 1812
TfiI GAWTC 6 cut(s) 616, 863, 1262, 1787, 1906, 2287
Tru1I TTAA 3 cut(s) 366, 1479, 1641
Tru9I TTAA 3 cut(s) 366, 1479, 1641
TscAI CASTG 5 cut(s) 124, 521, 889, 1262, 2117
TseFI GTSAC 3 cut(s) 776, 880, 1847
TseI GCWGC 4 cut(s) 1008, 1873, 2104, 2107
Tsp45I GTSAC 3 cut(s) 776, 880, 1847
TspDTI ATGAA 5 cut(s) 429, 1071, 1721, 1948, 1979
TspGWI ACGGA 2 cut(s) 1166, 2363
TspRI CASTG 5 cut(s) 124, 521, 889, 1262, 2117
Tth111I GACNNNGTC 1 cut(s) 494
VpaK11BI GGWCC 2 cut(s) 1587, 1681
XagI CCTNNNNNAGG 1 cut(s) 491
XapI RAATTY 4 cut(s) 764, 842, 849, 2363
XbaI TCTAGA 2 cut(s) 856, 866
XceI RCATGY 1 cut(s) 1495
XhoI CTCGAG 2 cut(s) 1177, 1910
XmiI GTMKAC 1 cut(s) 1320
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 5 cut(s) 483, 620, 857, 867, 1220
ZraI GACGTC 1 cut(s) 559
ZrmI AGTACT 1 cut(s) 946
Zsp2I ATGCAT 1 cut(s) 1616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.