RLG00000022381

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007697
Physical Location & Seq
Reverse (-)
82992672 .. 82995110
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022381

Sequence Viewer

Length: 2439 bp
ATGGATGAGGCTACCAACACCCCTAAGGCATTAAAGACTCCTAAGGCTTTAGGGACCCCCAAAGGCAATGTGACGGATCCATTCGAAAATCAAAAGGAAGATTCCGAGGAGAACAAAACGCAGGGACAAGAGAATGCTGATCAGAATGAGGAAGAGAAGGACAGTACCGGTGGCGAACACAGCGGTCCCCTTGAGCCTGTTGAGATACCTGAAGATCCCCATCCCACCCTCGAAAAGCTTTGTGATGACATTGAGCTGTGCATCTCTTCATTGCCTACACCTAACGAGAAAGATGAAAACCAACAAGAAGAGAAGGATAATGCAAATGAGGCAAAAGATACAAAAGGTGATGAGAAGGATGGGAAGGATGAAAAGGATACAAAAGATGACAAGGAAGAAAAGGATGTAAAAGATGAGGAGGGTGAAAAGGAAGACGAGAAGGGTGAGAAAGATGCAAAGGAGGGTGAAAAGAATGACAAGGAAGAAAAGGACGTGAAAGATGAGAATAAGGATGATGAAAAGGCTGCAGATCAGGATGAGAAAGAAATAGGTGACAAAGATGCAAAGGATGAGAAAGAGGCTGAAAAAGTCGTCGAAAAAGAAAAGGACCTCGTTCCAGACGAGCTCCTTAAGCTTATTGACAAGTTCTTGACTTGGGTAGATGATATCTGTTGCGAAGGCAGATGCAGGTGGGGAACGGTGCCTCATGACGACGAGGTGTTCTTGGAGGCTGTGGATCTGATGGCCAAGATGAGGGAGTCTCTCTTGTCCGTCTGTTCTATGGGATTGAAATTCCAAGAAAGGCACGGTGGTTTGATCAACCGCCTAAGTAGCATTCAGCACCGAGCCATGGCGTATCTGGAAGAAGAATTCAGAATCCTTCTAGAAGAATCCAAAACCGAGTCTGATCATCATCACCCTACAGCAGCTGATCACAACCATAACCATAACAGTAACAAAGACAGCAGCAGTGGTAACAAAGGAAAGAACGATCAAGAATCACAACCGGAATCCGATTCAGAAAATAAAGAAGGGGTTAATGTTGAATTTCCCGGGTTTCCTCAGGAGGTTGTGTCCAACCTGAATAAAATAGCCAACAAGATGATCTCCGCCGGCTACGAATCCGAGTGTTGCGAGGTCTACATCATCTCCAGGAGGCATGCATTCGATGAAAACCTCCACAAGCTCGGCCTAGAGAAGCACAGCATCGACGACGTGCAGAAGATGCACTGGGAAGCGCTGGAGAGAGAGATCATGTTGTGGGTCAAGGCATTCAAGCAATGCTCGCAATTCTACTTTTCCGGTGAGCGCCAGCTGGCAGAGTCGGTGTTTCCCGAGCCGGTGCTTTCCGAGCAGACGCCGATATGGTCGTGTCTGTTCAGCAATCTGAGCAGAGGAGTGATGATACAGCTCCTGAATTTCGCCGAAGGAATCGCCATGTCGAAAAGAGCGGCGGAGAAACTGTTCAAGACGCTCGATATGTACGAGGCGGTAAAGGAGATGCTCCCCAAGATAGACGAGCTTTTTCCGGAGGAGTGCGCCAGGGAGCTGAAGACGGAGACCACGACGGTGCTGAACCGGCTGGGCGAGGCGGCGATATTAATCTTCTGTGATCTGGAGAATTCCATCAAGGCCGAAACGGGTCGGAACCCGGTTCCGGGAGGGGCGGTCCACCCGTTGACCCGGTACACGATGAACTACCTGAGGTATGCGTGCGAGTACAAGGAGACGCTGGAGCTGGTGTTCAAGGAGCACACCAAGATCGAGCGGTCCGACTCGACGAGCAGGCCTGACGGTCGCGACGATTACGACGCGAGCGACACGTCGTTCGAGATACCCGACGACGAGTCGCCGTTCGCGGTGCAACTGGCGCGGGTGATGGACTTGTTGGACTCGAACCTGGAGGGGAAGGCGAAGCTGTACAAGGACGTGGCGTTGAGCTCGATTTTCATGATGAACAACGGGCGCTACATCTTGCAGAAGATCAAGGGCTCTCCGGAGATCAACGCCTGCATGGGCGACAAGTGGTGCCGGAAGAGGTCGACGGAGCTAAGGCAGTACCACAAGAACTACCAGAGGGAGACGTGGAGCCGGCTGTTGAGTTTTTTGAGCCACGAGGGGCTGAGCAACCACGGCAAAGTGAGCAAGCCTGTGCTGAAAGAGAGGTTCAAGAGCTTCAACGCTATGTTTGATGAGATTCATAAGACGCAGAGCACTTGGGTGGTGAGTGACGAGCAGCTTCAGTCGGAGCTTAGGGTTTCGATATCGGCGGTGGTCATTCCGGCGTACCGGTCGTTCTTGGGGAGATTCTCGCAGACGTTGGACCCGGGAAGGCAAACAGAGAAGTACATTAAGTTTCAGCCGGAAGATATAGAGACGTACATTGATGAGCTATTTGATGGCAACCCCAGTACCTCCATATTTAGGAAAAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

813

Amino Acids

92.84

Weight (kDa)

4.83

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 421 - 797 9.8e-126 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 678
Acc36I ACCTGC 1 cut(s) 678
AccB1I GGYRCC 2 cut(s) 700, 2028
AccBSI CCGCTC 2 cut(s) 1451, 1768
AccI GTMKAC 2 cut(s) 1140, 2042
AccII CGCG 4 cut(s) 1800, 1814, 1859, 1873
AccIII TCCGGA 2 cut(s) 1528, 1996
AclWI GGATC 4 cut(s) 71, 84, 209, 744
AcoI YGGCCR 1 cut(s) 744
AcsI RAATTY 5 cut(s) 791, 869, 1046, 1417, 1621
AcuI CTGAAG 3 cut(s) 231, 1571, 2225
AcyI GRCGYC 1 cut(s) 1358
AfeI AGCGCT 1 cut(s) 1239
AfiI CCNNNNNNNGG 5 cut(s) 753, 850, 1657, 1658, 2424
AflII CTTAAG 1 cut(s) 629
AflIII ACRYGT 1 cut(s) 1821
AgeI ACCGGT 2 cut(s) 167, 2289
AgsI TTSAA 7 cut(s) 790, 1046, 1276, 1468, 1747, 2170, 2179
AhdI GACNNNNNGTC 1 cut(s) 1846
AjiI CACGTC 5 cut(s) 493, 1216, 1824, 1930, 2085
AjnI CCWGG 3 cut(s) 1151, 1541, 1899
AleI CACNNNNGTG 2 cut(s) 1568, 2219
AloI GAACNNNNNNTCC 4 cut(s) 1448, 1480, 1727, 1759
Alw21I GWGCWC 4 cut(s) 627, 1755, 1943, 2216
Alw26I GTCTC 5 cut(s) 765, 1553, 1721, 2075, 2369
AlwI GGATC 4 cut(s) 71, 84, 209, 744
AlwNI CAGNNNCTG 2 cut(s) 929, 1414
Ama87I CYCGRG 3 cut(s) 1052, 1334, 2326
Aor13HI TCCGGA 2 cut(s) 1528, 1996
Aor51HI AGCGCT 1 cut(s) 1239
AoxI GGCC 4 cut(s) 744, 1189, 1632, 1787
ApeKI GCWGC 4 cut(s) 524, 926, 966, 2236
ApoI RAATTY 5 cut(s) 791, 869, 1046, 1417, 1621
ArsI GACNNNNNNTTYG 2 cut(s) 1811, 1843
AseI ATTAAT 1 cut(s) 1601
AsiGI ACCGGT 2 cut(s) 167, 2289
Asp700I GAANNNNTTC 1 cut(s) 1463
AspLEI GCGC 5 cut(s) 1240, 1311, 1541, 1873, 1968
AspS9I GGNCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
AsuC2I CCSGG 7 cut(s) 1053, 1054, 1652, 1659, 1684, 2327, 2328
AsuHPI GGTGA 9 cut(s) 359, 434, 455, 476, 563, 908, 1316, 1888, 2236
AsuII TTCGAA 1 cut(s) 84
AvaI CYCGRG 3 cut(s) 1052, 1334, 2326
AvaII GGWCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
AxyI CCTNAGG 4 cut(s) 24, 42, 1062, 1703
BaeI ACNNNNGTAYC 2 cut(s) 2395, 2428
BalI TGGCCA 1 cut(s) 746
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 2 cut(s) 700, 2028
BanII GRGCYC 3 cut(s) 627, 1943, 1994
BauI CACGAG 1 cut(s) 2114
BbsI GAAGAC 2 cut(s) 438, 1559
Bbv12I GWGCWC 4 cut(s) 627, 1755, 1943, 2216
BbvI GCAGC 4 cut(s) 511, 938, 978, 2248
BccI CCATC 6 cut(s) 228, 353, 736, 1634, 1873, 2393
BceAI ACGGC 2 cut(s) 1837, 2149
BciT130I CCWGG 3 cut(s) 1153, 1543, 1901
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 4 cut(s) 139, 816, 907, 931
BcnI CCSGG 7 cut(s) 1053, 1054, 1652, 1659, 1684, 2327, 2328
BcoDI GTCTC 5 cut(s) 765, 1553, 1721, 2075, 2369
BfaI CTAG 2 cut(s) 884, 1193
BfmI CTRYAG 2 cut(s) 525, 921
BfoI RGCGCY 3 cut(s) 1241, 1312, 1969
BfrI CTTAAG 1 cut(s) 629
BfuAI ACCTGC 1 cut(s) 678
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 6 cut(s) 525, 927, 967, 1452, 1593, 2237
BlpI GCTNAGC 1 cut(s) 2123
BlsI GCNGC 6 cut(s) 526, 928, 968, 1453, 1594, 2238
Bme18I GGWCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
BmeRI GACNNNNNGTC 1 cut(s) 1846
BmeT110I CYCGRG 3 cut(s) 1052, 1334, 2326
BmgBI CACGTC 5 cut(s) 493, 1216, 1824, 1930, 2085
BmgT120I GGNCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
BmrI ACTGGG 2 cut(s) 1240, 2403
BmsI GCATC 7 cut(s) 270, 442, 550, 674, 1215, 1215, 1491
BmuI ACTGGG 2 cut(s) 1240, 2403
BpiI GAAGAC 2 cut(s) 438, 1559
BplI GAGNNNNNCTC 2 cut(s) 745, 777
BpmI CTGGAG 5 cut(s) 1135, 1262, 1637, 1754, 1922
Bpu10I CCTNAGC 2 cut(s) 2051, 2252
Bpu1102I GCTNAGC 1 cut(s) 2123
Bpu14I TTCGAA 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 7 cut(s) 1053, 1054, 1652, 1659, 1684, 2327, 2328
BsaBI GATNNNNATC 3 cut(s) 219, 912, 1601
BsaHI GRCGYC 1 cut(s) 1358
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 6 cut(s) 105, 849, 1052, 1542, 2131, 2326
BsaWI WCCGGW 6 cut(s) 167, 1006, 1301, 1528, 1996, 2289
Bsc4I CCNNNNNNNGG 5 cut(s) 753, 850, 1657, 1658, 2424
Bse118I RCCGGY 6 cut(s) 167, 1112, 1339, 1578, 2091, 2289
Bse1I ACTGG 3 cut(s) 1235, 1872, 2409
Bse21I CCTNAGG 4 cut(s) 24, 42, 1062, 1703
Bse3DI GCAATG 3 cut(s) 73, 269, 1286
Bse8I GATNNNNATC 3 cut(s) 219, 912, 1601
BseAI TCCGGA 2 cut(s) 1528, 1996
BseBI CCWGG 3 cut(s) 1153, 1543, 1901
BseDI CCNNGG 6 cut(s) 105, 849, 1052, 1542, 2131, 2326
BseGI GGATG 8 cut(s) 10, 220, 364, 373, 409, 517, 541, 574
BseJI GATNNNNATC 3 cut(s) 219, 912, 1601
BseLI CCNNNNNNNGG 5 cut(s) 753, 850, 1657, 1658, 2424
BseMI GCAATG 3 cut(s) 73, 269, 1286
BseMII CTCAG 4 cut(s) 1076, 1379, 1694, 2114
BseNI ACTGG 3 cut(s) 1235, 1872, 2409
BseRI GAGGAG 4 cut(s) 122, 431, 1410, 1547
BseXI GCAGC 4 cut(s) 511, 938, 978, 2248
BseYI CCCAGC 1 cut(s) 1582
BsgI GTGCAG 1 cut(s) 1238
Bsh1236I CGCG 4 cut(s) 1800, 1814, 1859, 1873
Bsh1285I CGRYCG 2 cut(s) 1798, 2294
BshFI GGCC 4 cut(s) 746, 1191, 1634, 1789
BshNI GGYRCC 2 cut(s) 700, 2028
BshTI ACCGGT 2 cut(s) 167, 2289
BsiEI CGRYCG 2 cut(s) 1798, 2294
BsiHKAI GWGCWC 4 cut(s) 627, 1755, 1943, 2216
BsiHKCI CYCGRG 3 cut(s) 1052, 1334, 2326
BslFI GGGAC 3 cut(s) 67, 138, 171
BslI CCNNNNNNNGG 5 cut(s) 753, 850, 1657, 1658, 2424
BsmAI GTCTC 5 cut(s) 765, 1553, 1721, 2075, 2369
BsmBI CGTCTC 3 cut(s) 1721, 2075, 2369
BsmFI GGGAC 3 cut(s) 67, 138, 171
BsmI GAATGC 4 cut(s) 139, 834, 1163, 1271
BsnI GGCC 4 cut(s) 746, 1191, 1634, 1789
Bso31I GGTCTC 1 cut(s) 1553
BsoBI CYCGRG 3 cut(s) 1052, 1334, 2326
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 5 cut(s) 627, 1755, 1943, 1994, 2216
Bsp13I TCCGGA 2 cut(s) 1528, 1996
Bsp1407I TGTACA 1 cut(s) 1920
Bsp1720I GCTNAGC 1 cut(s) 2123
Bsp19I CCATGG 1 cut(s) 849
Bsp68I TCGCGA 1 cut(s) 1800
BspANI GGCC 4 cut(s) 746, 1191, 1634, 1789
BspCNI CTCAG 4 cut(s) 1075, 1380, 1695, 2115
BspEI TCCGGA 2 cut(s) 1528, 1996
BspFNI CGCG 4 cut(s) 1800, 1814, 1859, 1873
BspHI TCATGA 2 cut(s) 706, 1950
BspMAI CTGCAG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 678
BspPI GGATC 4 cut(s) 71, 84, 209, 744
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 2 cut(s) 700, 2028
BspTI CTTAAG 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 1553
BsrBI CCGCTC 2 cut(s) 1451, 1768
BsrDI GCAATG 3 cut(s) 73, 269, 1286
BsrFI RCCGGY 6 cut(s) 167, 1112, 1339, 1578, 2091, 2289
BsrGI TGTACA 1 cut(s) 1920
BsrI ACTGG 3 cut(s) 1235, 1872, 2409
BssAI RCCGGY 6 cut(s) 167, 1112, 1339, 1578, 2091, 2289
BssECI CCNNGG 6 cut(s) 105, 849, 1052, 1542, 2131, 2326
BssNI GRCGYC 1 cut(s) 1358
BssSI CACGAG 1 cut(s) 2114
BssT1I CCWWGG 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 2114
Bst2UI CCWGG 3 cut(s) 1153, 1543, 1901
Bst4CI ACNGT 7 cut(s) 164, 700, 809, 953, 1464, 1570, 1796
Bst6I CTCTTC 4 cut(s) 147, 271, 303, 2030
BstACI GRCGYC 1 cut(s) 1358
BstAFI CTTAAG 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstAUI TGTACA 1 cut(s) 1920
BstBI TTCGAA 1 cut(s) 84
BstDEI CTNAG 9 cut(s) 24, 42, 827, 1062, 1388, 1703, 2051, 2123, 2252
BstDSI CCRYGG 2 cut(s) 849, 2131
BstF5I GGATG 8 cut(s) 10, 220, 364, 373, 409, 517, 541, 574
BstFNI CGCG 4 cut(s) 1800, 1814, 1859, 1873
BstH2I RGCGCY 3 cut(s) 1241, 1312, 1969
BstHHI GCGC 5 cut(s) 1240, 1311, 1541, 1873, 1968
BstMAI GTCTC 5 cut(s) 765, 1553, 1721, 2075, 2369
BstMCI CGRYCG 2 cut(s) 1798, 2294
BstNI CCWGG 3 cut(s) 1153, 1543, 1901
BstNSI RCATGY 1 cut(s) 1163
BstSFI CTRYAG 2 cut(s) 525, 921
BstUI CGCG 4 cut(s) 1800, 1814, 1859, 1873
BstV1I GCAGC 4 cut(s) 511, 938, 978, 2248
BstV2I GAAGAC 2 cut(s) 438, 1559
BstX2I RGATCY 3 cut(s) 76, 214, 736
BstYI RGATCY 3 cut(s) 76, 214, 736
Bsu36I CCTNAGG 4 cut(s) 24, 42, 1062, 1703
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 4 cut(s) 746, 1191, 1634, 1789
BtgI CCRYGG 2 cut(s) 849, 2131
BtrI CACGTC 5 cut(s) 493, 1216, 1824, 1930, 2085
BtsCI GGATG 8 cut(s) 10, 220, 364, 373, 409, 517, 541, 574
BtsI GCAGTG 1 cut(s) 976
BtsIMutI CAGTG 2 cut(s) 976, 1228
BtuMI TCGCGA 1 cut(s) 1800
BveI ACCTGC 1 cut(s) 678
CaiI CAGNNNCTG 2 cut(s) 929, 1414
CciI TCATGA 2 cut(s) 706, 1950
CfoI GCGC 5 cut(s) 1240, 1311, 1541, 1873, 1968
Cfr10I RCCGGY 6 cut(s) 167, 1112, 1339, 1578, 2091, 2289
Cfr13I GGNCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
Cfr9I CCCGGG 2 cut(s) 1052, 2326
CpoI CGGWCCG 1 cut(s) 1770
CseI GACGC 5 cut(s) 1366, 1480, 1738, 1820, 2215
CspAI ACCGGT 2 cut(s) 167, 2289
CspI CGGWCCG 1 cut(s) 1770
CviAII CATG 7 cut(s) 707, 850, 1160, 1255, 1438, 1951, 2014
DdeI CTNAG 9 cut(s) 24, 42, 827, 1062, 1388, 1703, 2051, 2123, 2252
DriI GACNNNNNGTC 1 cut(s) 1846
EaeI YGGCCR 1 cut(s) 744
Eam1104I CTCTTC 4 cut(s) 147, 271, 303, 2030
Eam1105I GACNNNNNGTC 1 cut(s) 1846
EarI CTCTTC 4 cut(s) 147, 271, 303, 2030
EciI GGCGGA 2 cut(s) 1099, 1469
Ecl136II GAGCTC 2 cut(s) 625, 1941
Eco130I CCWWGG 1 cut(s) 849
Eco147I AGGCCT 1 cut(s) 1789
Eco24I GRGCYC 3 cut(s) 627, 1943, 1994
Eco31I GGTCTC 1 cut(s) 1553
Eco32I GATATC 2 cut(s) 667, 2265
Eco47I GGWCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
Eco47III AGCGCT 1 cut(s) 1239
Eco53kI GAGCTC 2 cut(s) 625, 1941
Eco57I CTGAAG 3 cut(s) 231, 1571, 2225
Eco81I CCTNAGG 4 cut(s) 24, 42, 1062, 1703
Eco88I CYCGRG 3 cut(s) 1052, 1334, 2326
EcoICRI GAGCTC 2 cut(s) 625, 1941
EcoO109I RGGNCCY 2 cut(s) 54, 607
EcoRI GAATTC 2 cut(s) 869, 1621
EcoRII CCWGG 3 cut(s) 1151, 1541, 1899
EcoRV GATATC 2 cut(s) 667, 2265
EcoT14I CCWWGG 1 cut(s) 849
EcoT22I ATGCAT 1 cut(s) 1165
EcoT38I GRGCYC 3 cut(s) 627, 1943, 1994
ErhI CCWWGG 1 cut(s) 849
Esp3I CGTCTC 3 cut(s) 1721, 2075, 2369
FaeI CATG 7 cut(s) 710, 853, 1163, 1258, 1441, 1954, 2017
FaqI GGGAC 3 cut(s) 67, 138, 171
FatI CATG 7 cut(s) 706, 849, 1159, 1254, 1437, 1950, 2013
FauI CCCGC 1 cut(s) 1866
FbaI TGATCA 4 cut(s) 139, 816, 907, 931
FblI GTMKAC 2 cut(s) 1140, 2042
Fnu4HI GCNGC 6 cut(s) 525, 927, 967, 1452, 1593, 2237
FokI GGATG 8 cut(s) 17, 207, 371, 380, 416, 524, 548, 581
FriOI GRGCYC 3 cut(s) 627, 1943, 1994
Fsp4HI GCNGC 6 cut(s) 525, 927, 967, 1452, 1593, 2237
FspBI CTAG 2 cut(s) 884, 1193
GlaI GCGC 5 cut(s) 1239, 1310, 1540, 1872, 1967
GluI GCNGC 6 cut(s) 525, 927, 967, 1452, 1593, 2237
GsaI CCCAGC 1 cut(s) 1586
GsuI CTGGAG 5 cut(s) 1135, 1262, 1637, 1754, 1922
HaeII RGCGCY 3 cut(s) 1241, 1312, 1969
HaeIII GGCC 4 cut(s) 746, 1191, 1634, 1789
HgaI GACGC 5 cut(s) 1366, 1480, 1738, 1820, 2215
HhaI GCGC 5 cut(s) 1240, 1311, 1541, 1873, 1968
Hin1I GRCGYC 1 cut(s) 1358
Hin1II CATG 7 cut(s) 710, 853, 1163, 1258, 1441, 1954, 2017
Hin6I GCGC 5 cut(s) 1238, 1309, 1539, 1871, 1966
HinP1I GCGC 5 cut(s) 1238, 1309, 1539, 1871, 1966
HincII GTYRAC 2 cut(s) 1680, 2043
HindII GTYRAC 2 cut(s) 1680, 2043
HindIII AAGCTT 2 cut(s) 236, 632
HphI GGTGA 9 cut(s) 359, 434, 455, 476, 563, 908, 1316, 1888, 2236
Hpy166II GTNNAC 5 cut(s) 1141, 1672, 1680, 1689, 2043
Hpy8I GTNNAC 5 cut(s) 1141, 1672, 1680, 1689, 2043
HpyCH4III ACNGT 7 cut(s) 164, 700, 809, 953, 1464, 1570, 1796
HpyCH4IV ACGT 7 cut(s) 492, 1215, 1823, 1929, 2084, 2318, 2378
HpyF3I CTNAG 9 cut(s) 24, 42, 827, 1062, 1388, 1703, 2051, 2123, 2252
HpySE526I ACGT 7 cut(s) 492, 1215, 1823, 1929, 2084, 2318, 2378
Hsp92I GRCGYC 1 cut(s) 1358
Hsp92II CATG 7 cut(s) 710, 853, 1163, 1258, 1441, 1954, 2017
HspAI GCGC 5 cut(s) 1238, 1309, 1539, 1871, 1966
KflI GGGWCCC 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 1528, 1996
KroI GCCGGC 2 cut(s) 1112, 2091
KroNI GCCGGC 2 cut(s) 1114, 2093
Ksp22I TGATCA 4 cut(s) 139, 816, 907, 931
LmnI GCTCC 9 cut(s) 630, 1416, 1509, 1546, 1735, 1750, 2047, 2088, 2248
Lsp1109I GCAGC 4 cut(s) 511, 938, 978, 2248
LweI GCATC 7 cut(s) 270, 442, 550, 674, 1215, 1215, 1491
MaeI CTAG 2 cut(s) 884, 1193
MaeII ACGT 7 cut(s) 492, 1215, 1823, 1929, 2084, 2318, 2378
MaeIII GTNAC 5 cut(s) 70, 551, 953, 974, 2228
MbiI CCGCTC 2 cut(s) 1451, 1768
MflI RGATCY 3 cut(s) 76, 214, 736
MhlI GDGCHC 5 cut(s) 627, 1755, 1943, 1994, 2216
MlsI TGGCCA 1 cut(s) 746
MluCI AATT 6 cut(s) 791, 869, 1046, 1289, 1417, 1621
MluNI TGGCCA 1 cut(s) 746
MlyI GAGTC 7 cut(s) 31, 767, 911, 1331, 1769, 1856, 1886
MmeI TCCRAC 6 cut(s) 1101, 1625, 1797, 1869, 2226, 2301
Mox20I TGGCCA 1 cut(s) 746
Mph1103I ATGCAT 1 cut(s) 1165
MroI TCCGGA 2 cut(s) 1528, 1996
MroNI GCCGGC 2 cut(s) 1112, 2091
MroXI GAANNNNTTC 1 cut(s) 1463
MscI TGGCCA 1 cut(s) 746
MseI TTAA 5 cut(s) 32, 630, 1038, 1601, 2352
MslI CAYNNNNRTG 2 cut(s) 1568, 2219
Msp20I TGGCCA 1 cut(s) 746
MspA1I CMGCKG 3 cut(s) 183, 929, 1315
MspCI CTTAAG 1 cut(s) 629
Mva1269I GAATGC 4 cut(s) 139, 834, 1163, 1271
MvaI CCWGG 3 cut(s) 1153, 1543, 1901
MvnI CGCG 4 cut(s) 1800, 1814, 1859, 1873
NaeI GCCGGC 2 cut(s) 1114, 2093
NciI CCSGG 7 cut(s) 1053, 1054, 1652, 1659, 1684, 2327, 2328
NcoI CCATGG 1 cut(s) 849
NgoMIV GCCGGC 2 cut(s) 1112, 2091
NlaIII CATG 7 cut(s) 710, 853, 1163, 1258, 1441, 1954, 2017
NmeAIII GCCGAG 1 cut(s) 1167
NmuCI GTSAC 3 cut(s) 70, 551, 2228
NruI TCGCGA 1 cut(s) 1800
NsiI ATGCAT 1 cut(s) 1165
NspI RCATGY 1 cut(s) 1163
NspV TTCGAA 1 cut(s) 84
OliI CACNNNNGTG 2 cut(s) 1568, 2219
PaeI GCATGC 1 cut(s) 1163
PagI TCATGA 2 cut(s) 706, 1950
PaqCI CACCTGC 1 cut(s) 678
PceI AGGCCT 1 cut(s) 1789
PcsI WCGNNNNNNNCGW 7 cut(s) 618, 1482, 1562, 1770, 1800, 1815, 1836
PctI GAATGC 4 cut(s) 139, 834, 1163, 1271
PdiI GCCGGC 2 cut(s) 1114, 2093
PdmI GAANNNNTTC 1 cut(s) 1463
PfoI TCCNGGA 2 cut(s) 1151, 1657
PinAI ACCGGT 2 cut(s) 167, 2289
PkrI GCNGC 6 cut(s) 526, 928, 968, 1453, 1594, 2238
PleI GAGTC 7 cut(s) 31, 766, 910, 1330, 1769, 1855, 1886
PpsI GAGTC 7 cut(s) 31, 766, 910, 1330, 1769, 1855, 1886
PpuMI RGGWCCY 2 cut(s) 54, 607
PshBI ATTAAT 1 cut(s) 1601
Psp124BI GAGCTC 2 cut(s) 627, 1943
Psp5II RGGWCCY 2 cut(s) 54, 607
Psp6I CCWGG 3 cut(s) 1151, 1541, 1899
PspFI CCCAGC 1 cut(s) 1582
PspGI CCWGG 3 cut(s) 1151, 1541, 1899
PspPI GGNCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
PspPPI RGGWCCY 2 cut(s) 54, 607
PsrI GAACNNNNNNTAC 2 cut(s) 2279, 2311
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 2 cut(s) 929, 1414
PsuI RGATCY 3 cut(s) 76, 214, 736
PvuII CAGCTG 2 cut(s) 929, 1315
RruI TCGCGA 1 cut(s) 1800
RseI CAYNNNNRTG 2 cut(s) 1568, 2219
Rsr2I CGGWCCG 1 cut(s) 1770
RsrII CGGWCCG 1 cut(s) 1770
SacI GAGCTC 2 cut(s) 627, 1943
SalI GTCGAC 1 cut(s) 2041
SaqAI TTAA 5 cut(s) 32, 630, 1038, 1601, 2352
SatI GCNGC 6 cut(s) 525, 927, 967, 1452, 1593, 2237
Sau96I GGNCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
SchI GAGTC 7 cut(s) 31, 767, 911, 1331, 1769, 1856, 1886
SduI GDGCHC 5 cut(s) 627, 1755, 1943, 1994, 2216
SfaNI GCATC 7 cut(s) 270, 442, 550, 674, 1215, 1215, 1491
SfcI CTRYAG 2 cut(s) 525, 921
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
SmaI CCCGGG 2 cut(s) 1054, 2328
SmiMI CAYNNNNRTG 2 cut(s) 1568, 2219
SmlI CTYRAG 2 cut(s) 191, 629
SmoI CTYRAG 2 cut(s) 191, 629
SphI GCATGC 1 cut(s) 1163
Sse9I AATT 6 cut(s) 791, 869, 1046, 1289, 1417, 1621
SseBI AGGCCT 1 cut(s) 1789
SspMI CTAG 2 cut(s) 884, 1193
SstI GAGCTC 2 cut(s) 627, 1943
StuI AGGCCT 1 cut(s) 1789
StyI CCWWGG 1 cut(s) 849
TaaI ACNGT 7 cut(s) 164, 700, 809, 953, 1464, 1570, 1796
TaiI ACGT 7 cut(s) 495, 1218, 1826, 1932, 2087, 2321, 2381
TasI AATT 6 cut(s) 791, 869, 1046, 1289, 1417, 1621
TatI WGTACW 3 cut(s) 1719, 1920, 2346
TauI GCSGC 2 cut(s) 1454, 1595
Tru1I TTAA 5 cut(s) 32, 630, 1038, 1601, 2352
Tru9I TTAA 5 cut(s) 32, 630, 1038, 1601, 2352
TscAI CASTG 2 cut(s) 976, 1235
TseFI GTSAC 3 cut(s) 70, 551, 2228
TseI GCWGC 4 cut(s) 524, 926, 966, 2236
Tsp45I GTSAC 3 cut(s) 70, 551, 2228
TspDTI ATGAA 9 cut(s) 258, 309, 384, 531, 1185, 1709, 1939, 1970, 2189
TspGWI ACGGA 4 cut(s) 89, 760, 1571, 2060
TspMI CCCGGG 2 cut(s) 1052, 2326
TspRI CASTG 2 cut(s) 976, 1235
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 6 cut(s) 54, 185, 607, 1669, 1770, 2323
VspI ATTAAT 1 cut(s) 1601
XapI RAATTY 5 cut(s) 791, 869, 1046, 1417, 1621
XbaI TCTAGA 1 cut(s) 883
XceI RCATGY 1 cut(s) 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XmaI CCCGGG 2 cut(s) 1052, 2326
XmiI GTMKAC 2 cut(s) 1140, 2042
XmnI GAANNNNTTC 1 cut(s) 1463
XspI CTAG 2 cut(s) 884, 1193
Zsp2I ATGCAT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.