Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0007697

Physical Location & Seq

Genomic Coordinates Forward (+)

507546 .. 509981

Gene Structure 2436 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00076770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGGATGAGGCTACCAACACCCCTAAGGCATTAAAGACTCCTAAGGCTTTAGGGACCCCCAAGGGCAATGTGACGGATCCATTCGAAAATCAAAAGGAAGATTCCGAGGAGAACAAAAAGCAGGGACAAGAGAATGCTGATCAGAAGGAGGAAGAGAAGGACAGTACCGGTGGCGAACACAGCGGTCCCCTTGAGCCGGTTGACATACCGGAAGATCCCCACCCCACCCTCGAAAAGCTTTGTGATGACATTGAGCGATGCATCTCTTCATTGCCTACACCTAACGAGAAAGATGAAAACCAACAAGAAGAGAAGGATACTGCAAATGAGGCAAAAGATACAAAAGGTGATGAGAAGGATGGGAGGGATGAAAAGGATACAAAAGATGACAAGGAAGAAAAGGATGTAAAGGATGAGAAGGGTGAAAAGGAAGAAGAGAAGGGTGAGAAAGATGCAAAGGAGGGTGAAAAGAATGACAAGGAAGAAAAGGATGTGAAAGATGAGAATACGGATGATGAAAAGGCTGCAGATCAGGATGAGAGAGAATTAGGTGACAAAGATGCAAAGGATGAGAAAGAGGCTGAAGAGGTCGTCGAAAAAGAAAAGGATCTCGTTCCAGAGGAGCTCCTTAAGCTTATTGACAAGTTCTTGACTTGGGTAGATGATATCTGTTGCGAAGGCAGATGCAGGTGGGGAACGGTGCCTCAAGACGACGAGGCGTTCTTGGAGGCTGTGGATCTGATGGCCAAGATGAGGGAGTCTCTCTTGTCCGTCTGTTCTATGGGATTGAAATTCCAAGAAAGGCACGGCGGTTTGATCAACCGCCTAAGTAGCATTCAGCACCGAGCCATGGCCTATCTGGAAGAAGAATTCAGAATCCTTCTAGAAGAATCCAAAACCGAGTCTGATCATCATTACTCTACAGCAGCTGATCACAACCATAACCATAACAGTAACAAAGACAGCAGCAGTGGTAGCAAAGGAAAGAACGATCAAGAATCACAACCGGAATCCGAGTCAGAAAATAAGGAAGGGGTTGTCGAATTTCCCGGGTTTCCTGAGGAGGTTGTGTCCAACCTGAATAAAATAGCCAACAAGATGATCTCCGCCGGCTACGAATCCGAGTGTTGCGAGGTCTACATCATCTCCAGGAGGCATGCATTCGATGAAAACCTCCACAAGCTCGGCCTAGAGAAGCACAGCATCGACGACGTGCAGAAAATGCACTGGGAAGCGCTGGAGAGAGAGATCATGTTGTGGGTCAAGGCCTTGAAGCAATGCTCGCAATTCTACTTTTCCGGTGAGCGTCAGCTGGCAGAGTCGGTGTTTCCGGAGCCGGTGCTTTCCGAGCAGACGCCGATATGGTCGTGTCTGTTCAGCAATCTGAGCAGAGGAGTGATGATACAGCTCCTGAATTTCGCCGAAGGAATTGCCATGTCCAAAAGAGCGGCGGAGAAACTGTTCAAGACGCTCGATATGTACGAGGCGGTAAAGGAGATGCTCCCCAAGATAGACGAGCTTTTTCCGGAGGAGTGCGCCAGGGAGCTGAAGACGGAGACCACGACAGTGCTGAACCGGCTGGGCGAGGCGGCGATATTAATCTTCTGTGATCTGGAGAATTCCATCAAGGCCGAAACGGGTCGGAACCCGGTTCCGGGAGGGGCGGTCCACCCGTTGACCCGGTACACAATGAACTACCTGAGATACGCGTGCGAGTACAAGGAGACGCTGGAGCTGGTGTTCAAAGAGCACACCAAGATCGAGCGGTCCGACTCGACGAGCAGGCCTGACGGTCGCGACGATTACGACGCGAGCGACACGTCGTTTGAGATACCCGACGACGAGTCGCCGTTCGCGGTGCAACTGGCGCGGGTGATGGACTTGTTGGACTCGAACCTGGAGGGGAAGGCGAAGCTGTACAAGGACGTGGCGTTGAGCTCGATTTTCATGATGAACAACGGGCGCTACATCTTGCAGAAGATCAAGGGCTCTCCGGAGATCAACGCCTGCATGGGCGACAAGTGGTGCCGGAAGAGGTCGACGGAGCTGAGGCAGTACCACAAGAACTACCAGAGAGAGACGTGGAGCCGGCTGTTGAGTTTTTTGAGCCACGAGGGGCTGAGCAACCACGGCAAAGTGAGCAAGCCTGTGCTGAAAGAGAGGTTCAAGAGCTTCAACGCTATGTTTGATGAGATTCATAAGACGCAGAGCACGTGGGTGGTGAGTGACGAGCAGCTTCAGTCGGAGCTTAGGGTTTCGATATCGGCGGTGGTCATTCCGGCGTATCGGTCGTTCTTGGGGAGATTCTCGCAGACGTTGGACCCGGGAAGGCAAACAGAGAAGTACATTAAGTTTCAGCCGGAAGATATAGAGACGTACATTGATGAGCTATTTGATGGCAACCCCAGTACCTCCATATTTAGGAAAAAACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Exo70

Protein Analysis

811

Amino Acids

92.77

Weight (kDa)

4.83

Isoelectric Point (pI)

48.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo70_N	PF20669	237 - 297	8e-06	Exocyst complex component Exo70 N-terminal
Exo70_C	PF03081	420 - 796	1.3e-125	Exo70 exocyst complex subunit C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13990
fragaria_vesca	FvH4_6g53940
malus_domestica	MD00G1220500.v1.1 MD09G1007100.v1.1 MD10G1123900.v1.1 MD10G1124000.v1.1 MD17G1010900.v1.1 MD17G1254300.v1.1
prunus_persica	Prupe.3G310000_v2.0.a1
pyrus_communis	pycom111g00530 pycom17g00560
rosa_chinensis	RchiOBHm_Chr2g0176101
rosa_laevigata	RLG00000022381
rosa_multiflora	Rmu_co8050736.1_g000001 Rmu_sc0009879.1_g000001 Rmu_sc0012674.1_g000001 Rmu_sc0041131.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076770
rosa_rugosa	Rorug02G0590700
rosa_samantha	Rh2AG669300 Rh2BG680900 Rh2CG643800 Rh2DG694600
rosa_wichuraiana	Rw2G054900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	678
Acc36I	ACCTGC	1 cut(s)	678
AccB1I	GGYRCC	2 cut(s)	700, 2025
AccBSI	CCGCTC	2 cut(s)	1448, 1765
AccI	GTMKAC	2 cut(s)	1137, 2039
AccII	CGCG	5 cut(s)	1709, 1797, 1811, 1856, 1870
AccIII	TCCGGA	3 cut(s)	1330, 1525, 1993
AclWI	GGATC	5 cut(s)	71, 84, 209, 615, 744
AcoI	YGGCCR	1 cut(s)	744
AcsI	RAATTY	5 cut(s)	791, 869, 1043, 1414, 1618
AcuI	CTGAAG	3 cut(s)	603, 1568, 2222
AcvI	CACGTG	1 cut(s)	2214
AcyI	GRCGYC	1 cut(s)	1355
AfaI	GTAC	9 cut(s)	166, 1481, 1685, 1718, 1919, 2057, 2345, 2378, 2410
AfeI	AGCGCT	1 cut(s)	1236
AfiI	CCNNNNNNNGG	6 cut(s)	196, 753, 850, 1654, 1655, 2421
AflII	CTTAAG	1 cut(s)	629
AflIII	ACRYGT	2 cut(s)	1707, 1818
AgeI	ACCGGT	1 cut(s)	167
AgsI	TTSAA	6 cut(s)	790, 1273, 1465, 1744, 2167, 2176
AhdI	GACNNNNNGTC	1 cut(s)	1843
AjiI	CACGTC	4 cut(s)	1213, 1821, 1927, 2082
AjnI	CCWGG	3 cut(s)	1148, 1538, 1896
AleI	CACNNNNGTG	2 cut(s)	1565, 2216
AloI	GAACNNNNNNTCC	4 cut(s)	1445, 1477, 1724, 1756
Alw21I	GWGCWC	4 cut(s)	627, 1752, 1940, 2213
Alw26I	GTCTC	5 cut(s)	765, 1550, 1718, 2072, 2366
AlwI	GGATC	5 cut(s)	71, 84, 209, 615, 744
AlwNI	CAGNNNCTG	2 cut(s)	929, 1411
Ama87I	CYCGRG	2 cut(s)	1049, 2323
Aor13HI	TCCGGA	3 cut(s)	1330, 1525, 1993
Aor51HI	AGCGCT	1 cut(s)	1236
AoxI	GGCC	6 cut(s)	744, 852, 1186, 1266, 1629, 1784
ApeKI	GCWGC	4 cut(s)	524, 926, 966, 2233
ApoI	RAATTY	5 cut(s)	791, 869, 1043, 1414, 1618
ArsI	GACNNNNNNTTYG	2 cut(s)	1808, 1840
AseI	ATTAAT	1 cut(s)	1598
AsiGI	ACCGGT	1 cut(s)	167
Asp700I	GAANNNNTTC	1 cut(s)	1460
AspLEI	GCGC	4 cut(s)	1237, 1538, 1870, 1965
AspS9I	GGNCC	5 cut(s)	54, 185, 1666, 1767, 2320
AsuC2I	CCSGG	7 cut(s)	1050, 1051, 1649, 1656, 1681, 2324, 2325
AsuHPI	GGTGA	8 cut(s)	359, 434, 455, 476, 563, 1313, 1885, 2233
AsuII	TTCGAA	1 cut(s)	84
AvaI	CYCGRG	2 cut(s)	1049, 2323
AvaII	GGWCC	5 cut(s)	54, 185, 1666, 1767, 2320
AxyI	CCTNAGG	3 cut(s)	24, 42, 1059
BaeI	ACNNNNGTAYC	2 cut(s)	2392, 2425
BalI	TGGCCA	1 cut(s)	746
BamHI	GGATCC	1 cut(s)	76
BanI	GGYRCC	2 cut(s)	700, 2025
BanII	GRGCYC	3 cut(s)	627, 1940, 1991
BauI	CACGAG	1 cut(s)	2111
BbrPI	CACGTG	1 cut(s)	2214
BbsI	GAAGAC	1 cut(s)	1556
Bbv12I	GWGCWC	4 cut(s)	627, 1752, 1940, 2213
BbvCI	CCTCAGC	1 cut(s)	2048
BbvI	GCAGC	4 cut(s)	511, 938, 978, 2245
BccI	CCATC	5 cut(s)	353, 736, 1631, 1870, 2390
BceAI	ACGGC	3 cut(s)	823, 1834, 2146
BcgI	CGANNNNNNTGC	2 cut(s)	1412, 1446
BciT130I	CCWGG	3 cut(s)	1150, 1540, 1898
BciVI	GTATCC	2 cut(s)	310, 370
BclI	TGATCA	4 cut(s)	139, 816, 907, 931
BcnI	CCSGG	7 cut(s)	1050, 1051, 1649, 1656, 1681, 2324, 2325
BcoDI	GTCTC	5 cut(s)	765, 1550, 1718, 2072, 2366
BfaI	CTAG	2 cut(s)	884, 1190
BfmI	CTRYAG	2 cut(s)	525, 921
BfoI	RGCGCY	2 cut(s)	1238, 1966
BfrI	CTTAAG	1 cut(s)	629
BfuAI	ACCTGC	1 cut(s)	678
BfuI	GTATCC	2 cut(s)	310, 370
BisI	GCNGC	6 cut(s)	525, 927, 967, 1449, 1590, 2234
BlpI	GCTNAGC	1 cut(s)	2120
BlsI	GCNGC	6 cut(s)	526, 928, 968, 1450, 1591, 2235
Bme18I	GGWCC	5 cut(s)	54, 185, 1666, 1767, 2320
BmeRI	GACNNNNNGTC	1 cut(s)	1843
BmeT110I	CYCGRG	2 cut(s)	1049, 2323
BmgBI	CACGTC	4 cut(s)	1213, 1821, 1927, 2082
BmgT120I	GGNCC	5 cut(s)	54, 185, 1666, 1767, 2320
BmrI	ACTGGG	2 cut(s)	1237, 2400
BmsI	GCATC	7 cut(s)	248, 270, 442, 550, 674, 1212, 1488
BmuI	ACTGGG	2 cut(s)	1237, 2400
BpiI	GAAGAC	1 cut(s)	1556
BplI	GAGNNNNNCTC	2 cut(s)	745, 777
BpmI	CTGGAG	5 cut(s)	1132, 1259, 1634, 1751, 1919
Bpu10I	CCTNAGC	2 cut(s)	2048, 2249
Bpu1102I	GCTNAGC	1 cut(s)	2120
Bpu14I	TTCGAA	1 cut(s)	84
BpuEI	CTTGAG	2 cut(s)	212, 690
BpuMI	CCSGG	7 cut(s)	1050, 1051, 1649, 1656, 1681, 2324, 2325
BsaAI	YACGTR	1 cut(s)	2214
BsaBI	GATNNNNATC	1 cut(s)	1598
BsaHI	GRCGYC	1 cut(s)	1355
BsaI	GGTCTC	1 cut(s)	1550
BsaJI	CCNNGG	7 cut(s)	60, 105, 849, 1049, 1539, 2128, 2323
BsaWI	WCCGGW	7 cut(s)	167, 208, 1006, 1298, 1330, 1525, 1993
Bsc4I	CCNNNNNNNGG	6 cut(s)	196, 753, 850, 1654, 1655, 2421
Bse118I	RCCGGY	6 cut(s)	167, 196, 1109, 1336, 1575, 2088
Bse1I	ACTGG	3 cut(s)	1232, 1869, 2406
Bse21I	CCTNAGG	3 cut(s)	24, 42, 1059
Bse3DI	GCAATG	3 cut(s)	73, 269, 1283
Bse8I	GATNNNNATC	1 cut(s)	1598
BseAI	TCCGGA	3 cut(s)	1330, 1525, 1993
BseBI	CCWGG	3 cut(s)	1150, 1540, 1898
BseDI	CCNNGG	7 cut(s)	60, 105, 849, 1049, 1539, 2128, 2323
BseGI	GGATG	9 cut(s)	10, 364, 373, 409, 418, 496, 517, 541, 574
BseJI	GATNNNNATC	1 cut(s)	1598
BseLI	CCNNNNNNNGG	6 cut(s)	196, 753, 850, 1654, 1655, 2421
BseMI	GCAATG	3 cut(s)	73, 269, 1283
BseMII	CTCAG	5 cut(s)	1050, 1376, 1691, 2039, 2111
BseNI	ACTGG	3 cut(s)	1232, 1869, 2406
BseRI	GAGGAG	5 cut(s)	122, 635, 1076, 1407, 1544
BseXI	GCAGC	4 cut(s)	511, 938, 978, 2245
BseYI	CCCAGC	1 cut(s)	1579
BsgI	GTGCAG	1 cut(s)	1235
Bsh1236I	CGCG	5 cut(s)	1709, 1797, 1811, 1856, 1870
Bsh1285I	CGRYCG	2 cut(s)	1795, 2291
BshFI	GGCC	6 cut(s)	746, 854, 1188, 1268, 1631, 1786
BshNI	GGYRCC	2 cut(s)	700, 2025
BshTI	ACCGGT	1 cut(s)	167
BsiEI	CGRYCG	2 cut(s)	1795, 2291
BsiHKAI	GWGCWC	4 cut(s)	627, 1752, 1940, 2213
BsiHKCI	CYCGRG	2 cut(s)	1049, 2323
BslFI	GGGAC	3 cut(s)	67, 138, 171
BslI	CCNNNNNNNGG	6 cut(s)	196, 753, 850, 1654, 1655, 2421
BsmAI	GTCTC	5 cut(s)	765, 1550, 1718, 2072, 2366
BsmBI	CGTCTC	3 cut(s)	1718, 2072, 2366
BsmFI	GGGAC	3 cut(s)	67, 138, 171
BsmI	GAATGC	3 cut(s)	139, 834, 1160
BsnI	GGCC	6 cut(s)	746, 854, 1188, 1268, 1631, 1786
Bso31I	GGTCTC	1 cut(s)	1550
BsoBI	CYCGRG	2 cut(s)	1049, 2323
Bsp119I	TTCGAA	1 cut(s)	84
Bsp1286I	GDGCHC	5 cut(s)	627, 1752, 1940, 1991, 2213
Bsp13I	TCCGGA	3 cut(s)	1330, 1525, 1993
Bsp1407I	TGTACA	1 cut(s)	1917
Bsp1720I	GCTNAGC	1 cut(s)	2120
Bsp19I	CCATGG	1 cut(s)	849
Bsp68I	TCGCGA	1 cut(s)	1797
BspANI	GGCC	6 cut(s)	746, 854, 1188, 1268, 1631, 1786
BspCNI	CTCAG	5 cut(s)	1051, 1377, 1692, 2040, 2112
BspEI	TCCGGA	3 cut(s)	1330, 1525, 1993
BspFNI	CGCG	5 cut(s)	1709, 1797, 1811, 1856, 1870
BspHI	TCATGA	1 cut(s)	1947
BspMAI	CTGCAG	1 cut(s)	529
BspMI	ACCTGC	1 cut(s)	678
BspPI	GGATC	5 cut(s)	71, 84, 209, 615, 744
BspT104I	TTCGAA	1 cut(s)	84
BspT107I	GGYRCC	2 cut(s)	700, 2025
BspTI	CTTAAG	1 cut(s)	629
BspTNI	GGTCTC	1 cut(s)	1550
BsrBI	CCGCTC	2 cut(s)	1448, 1765
BsrDI	GCAATG	3 cut(s)	73, 269, 1283
BsrFI	RCCGGY	6 cut(s)	167, 196, 1109, 1336, 1575, 2088
BsrGI	TGTACA	1 cut(s)	1917
BsrI	ACTGG	3 cut(s)	1232, 1869, 2406
BssAI	RCCGGY	6 cut(s)	167, 196, 1109, 1336, 1575, 2088
BssECI	CCNNGG	7 cut(s)	60, 105, 849, 1049, 1539, 2128, 2323
BssNI	GRCGYC	1 cut(s)	1355
BssSI	CACGAG	1 cut(s)	2111
BssT1I	CCWWGG	2 cut(s)	60, 849
Bst2BI	CACGAG	1 cut(s)	2111
Bst2UI	CCWGG	3 cut(s)	1150, 1540, 1898
Bst4CI	ACNGT	6 cut(s)	164, 700, 953, 1461, 1567, 1793
Bst6I	CTCTTC	6 cut(s)	147, 271, 303, 429, 579, 2027
BstACI	GRCGYC	1 cut(s)	1355
BstAFI	CTTAAG	1 cut(s)	629
BstAPI	GCANNNNNTGC	1 cut(s)	1222
BstAUI	TGTACA	1 cut(s)	1917
BstBAI	YACGTR	1 cut(s)	2214
BstBI	TTCGAA	1 cut(s)	84
BstDEI	CTNAG	9 cut(s)	24, 42, 827, 1059, 1385, 1700, 2048, 2120, 2249
BstDSI	CCRYGG	2 cut(s)	849, 2128
BstF5I	GGATG	9 cut(s)	10, 364, 373, 409, 418, 496, 517, 541, 574
BstFNI	CGCG	5 cut(s)	1709, 1797, 1811, 1856, 1870
BstH2I	RGCGCY	2 cut(s)	1238, 1966
BstHHI	GCGC	4 cut(s)	1237, 1538, 1870, 1965
BstMAI	GTCTC	5 cut(s)	765, 1550, 1718, 2072, 2366
BstMCI	CGRYCG	2 cut(s)	1795, 2291
BstNI	CCWGG	3 cut(s)	1150, 1540, 1898
BstNSI	RCATGY	1 cut(s)	1160
BstSFI	CTRYAG	2 cut(s)	525, 921
BstUI	CGCG	5 cut(s)	1709, 1797, 1811, 1856, 1870
BstV1I	GCAGC	4 cut(s)	511, 938, 978, 2245
BstV2I	GAAGAC	1 cut(s)	1556
BstX2I	RGATCY	4 cut(s)	76, 214, 607, 736
BstYI	RGATCY	4 cut(s)	76, 214, 607, 736
Bsu36I	CCTNAGG	3 cut(s)	24, 42, 1059
BsuI	GTATCC	2 cut(s)	310, 370
BsuRI	GGCC	6 cut(s)	746, 854, 1188, 1268, 1631, 1786
BtgI	CCRYGG	2 cut(s)	849, 2128
BtgZI	GCGATG	1 cut(s)	271
BtrI	CACGTC	4 cut(s)	1213, 1821, 1927, 2082
BtsCI	GGATG	9 cut(s)	10, 364, 373, 409, 418, 496, 517, 541, 574
BtsI	GCAGTG	1 cut(s)	976
BtsIMutI	CAGTG	3 cut(s)	976, 1225, 1572
BtuMI	TCGCGA	1 cut(s)	1797
BveI	ACCTGC	1 cut(s)	678
CaiI	CAGNNNCTG	2 cut(s)	929, 1411
CciI	TCATGA	1 cut(s)	1947
CfoI	GCGC	4 cut(s)	1237, 1538, 1870, 1965
Cfr10I	RCCGGY	6 cut(s)	167, 196, 1109, 1336, 1575, 2088
Cfr13I	GGNCC	5 cut(s)	54, 185, 1666, 1767, 2320
Cfr9I	CCCGGG	2 cut(s)	1049, 2323
CpoI	CGGWCCG	1 cut(s)	1767
CseI	GACGC	6 cut(s)	1295, 1363, 1477, 1735, 1817, 2212
Csp6I	GTAC	9 cut(s)	165, 1480, 1684, 1717, 1918, 2056, 2344, 2377, 2409
CspAI	ACCGGT	1 cut(s)	167
CspI	CGGWCCG	1 cut(s)	1767
CviAII	CATG	6 cut(s)	850, 1157, 1252, 1435, 1948, 2011
CviQI	GTAC	9 cut(s)	165, 1480, 1684, 1717, 1918, 2056, 2344, 2377, 2409
DdeI	CTNAG	9 cut(s)	24, 42, 827, 1059, 1385, 1700, 2048, 2120, 2249
DriI	GACNNNNNGTC	1 cut(s)	1843
EaeI	YGGCCR	1 cut(s)	744
Eam1104I	CTCTTC	6 cut(s)	147, 271, 303, 429, 579, 2027
Eam1105I	GACNNNNNGTC	1 cut(s)	1843
EarI	CTCTTC	6 cut(s)	147, 271, 303, 429, 579, 2027
EciI	GGCGGA	2 cut(s)	1096, 1466
Ecl136II	GAGCTC	2 cut(s)	625, 1938
Eco130I	CCWWGG	2 cut(s)	60, 849
Eco147I	AGGCCT	2 cut(s)	1268, 1786
Eco24I	GRGCYC	3 cut(s)	627, 1940, 1991
Eco31I	GGTCTC	1 cut(s)	1550
Eco32I	GATATC	2 cut(s)	667, 2262
Eco47I	GGWCC	5 cut(s)	54, 185, 1666, 1767, 2320
Eco47III	AGCGCT	1 cut(s)	1236
Eco53kI	GAGCTC	2 cut(s)	625, 1938
Eco57I	CTGAAG	3 cut(s)	603, 1568, 2222
Eco72I	CACGTG	1 cut(s)	2214
Eco81I	CCTNAGG	3 cut(s)	24, 42, 1059
Eco88I	CYCGRG	2 cut(s)	1049, 2323
EcoICRI	GAGCTC	2 cut(s)	625, 1938
EcoO109I	RGGNCCY	1 cut(s)	54
EcoRI	GAATTC	2 cut(s)	869, 1618
EcoRII	CCWGG	3 cut(s)	1148, 1538, 1896
EcoRV	GATATC	2 cut(s)	667, 2262
EcoT14I	CCWWGG	2 cut(s)	60, 849
EcoT22I	ATGCAT	2 cut(s)	263, 1162
EcoT38I	GRGCYC	3 cut(s)	627, 1940, 1991
ErhI	CCWWGG	2 cut(s)	60, 849
Esp3I	CGTCTC	3 cut(s)	1718, 2072, 2366
FaeI	CATG	6 cut(s)	853, 1160, 1255, 1438, 1951, 2014
FaqI	GGGAC	3 cut(s)	67, 138, 171
FatI	CATG	6 cut(s)	849, 1156, 1251, 1434, 1947, 2010
FauI	CCCGC	1 cut(s)	1863
FbaI	TGATCA	4 cut(s)	139, 816, 907, 931
FblI	GTMKAC	2 cut(s)	1137, 2039
Fnu4HI	GCNGC	6 cut(s)	525, 927, 967, 1449, 1590, 2234
FokI	GGATG	9 cut(s)	17, 371, 380, 416, 425, 503, 524, 548, 581
FriOI	GRGCYC	3 cut(s)	627, 1940, 1991
Fsp4HI	GCNGC	6 cut(s)	525, 927, 967, 1449, 1590, 2234
FspBI	CTAG	2 cut(s)	884, 1190
GlaI	GCGC	4 cut(s)	1236, 1537, 1869, 1964
GluI	GCNGC	6 cut(s)	525, 927, 967, 1449, 1590, 2234
GsaI	CCCAGC	1 cut(s)	1583
GsuI	CTGGAG	5 cut(s)	1132, 1259, 1634, 1751, 1919
HaeII	RGCGCY	2 cut(s)	1238, 1966
HaeIII	GGCC	6 cut(s)	746, 854, 1188, 1268, 1631, 1786
HgaI	GACGC	6 cut(s)	1295, 1363, 1477, 1735, 1817, 2212
HhaI	GCGC	4 cut(s)	1237, 1538, 1870, 1965
Hin1I	GRCGYC	1 cut(s)	1355
Hin1II	CATG	6 cut(s)	853, 1160, 1255, 1438, 1951, 2014
Hin6I	GCGC	4 cut(s)	1235, 1536, 1868, 1963
HinP1I	GCGC	4 cut(s)	1235, 1536, 1868, 1963
HincII	GTYRAC	3 cut(s)	202, 1677, 2040
HindII	GTYRAC	3 cut(s)	202, 1677, 2040
HindIII	AAGCTT	2 cut(s)	236, 632
HphI	GGTGA	8 cut(s)	359, 434, 455, 476, 563, 1313, 1885, 2233
Hpy166II	GTNNAC	6 cut(s)	202, 1138, 1669, 1677, 1686, 2040
Hpy8I	GTNNAC	6 cut(s)	202, 1138, 1669, 1677, 1686, 2040
HpyCH4III	ACNGT	6 cut(s)	164, 700, 953, 1461, 1567, 1793
HpyCH4IV	ACGT	7 cut(s)	1212, 1820, 1926, 2081, 2213, 2315, 2375
HpyF3I	CTNAG	9 cut(s)	24, 42, 827, 1059, 1385, 1700, 2048, 2120, 2249
HpySE526I	ACGT	7 cut(s)	1212, 1820, 1926, 2081, 2213, 2315, 2375
Hsp92I	GRCGYC	1 cut(s)	1355
Hsp92II	CATG	6 cut(s)	853, 1160, 1255, 1438, 1951, 2014
HspAI	GCGC	4 cut(s)	1235, 1536, 1868, 1963
KflI	GGGWCCC	1 cut(s)	54
Kpn2I	TCCGGA	3 cut(s)	1330, 1525, 1993
KroI	GCCGGC	2 cut(s)	1109, 2088
KroNI	GCCGGC	2 cut(s)	1111, 2090
Ksp22I	TGATCA	4 cut(s)	139, 816, 907, 931
Lsp1109I	GCAGC	4 cut(s)	511, 938, 978, 2245
LweI	GCATC	7 cut(s)	248, 270, 442, 550, 674, 1212, 1488
MaeI	CTAG	2 cut(s)	884, 1190
MaeII	ACGT	7 cut(s)	1212, 1820, 1926, 2081, 2213, 2315, 2375
MaeIII	GTNAC	4 cut(s)	70, 551, 953, 2225
MbiI	CCGCTC	2 cut(s)	1448, 1765
MflI	RGATCY	4 cut(s)	76, 214, 607, 736
MhlI	GDGCHC	5 cut(s)	627, 1752, 1940, 1991, 2213
MlsI	TGGCCA	1 cut(s)	746
MluCI	AATT	8 cut(s)	545, 791, 869, 1043, 1286, 1414, 1428, 1618
MluI	ACGCGT	1 cut(s)	1707
MluNI	TGGCCA	1 cut(s)	746
MlyI	GAGTC	8 cut(s)	31, 767, 911, 1025, 1328, 1766, 1853, 1883
MmeI	TCCRAC	6 cut(s)	1098, 1622, 1794, 1866, 2223, 2298
Mox20I	TGGCCA	1 cut(s)	746
Mph1103I	ATGCAT	2 cut(s)	263, 1162
MroI	TCCGGA	3 cut(s)	1330, 1525, 1993
MroNI	GCCGGC	2 cut(s)	1109, 2088
MroXI	GAANNNNTTC	1 cut(s)	1460
MscI	TGGCCA	1 cut(s)	746
MseI	TTAA	4 cut(s)	32, 630, 1598, 2349
MslI	CAYNNNNRTG	2 cut(s)	1565, 2216
Msp20I	TGGCCA	1 cut(s)	746
MspA1I	CMGCKG	3 cut(s)	183, 929, 1312
MspCI	CTTAAG	1 cut(s)	629
Mva1269I	GAATGC	3 cut(s)	139, 834, 1160
MvaI	CCWGG	3 cut(s)	1150, 1540, 1898
MvnI	CGCG	5 cut(s)	1709, 1797, 1811, 1856, 1870
NaeI	GCCGGC	2 cut(s)	1111, 2090
NciI	CCSGG	7 cut(s)	1050, 1051, 1649, 1656, 1681, 2324, 2325
NcoI	CCATGG	1 cut(s)	849
NgoMIV	GCCGGC	2 cut(s)	1109, 2088
NlaIII	CATG	6 cut(s)	853, 1160, 1255, 1438, 1951, 2014
NmeAIII	GCCGAG	1 cut(s)	1164
NmuCI	GTSAC	3 cut(s)	70, 551, 2225
NruI	TCGCGA	1 cut(s)	1797
NsiI	ATGCAT	2 cut(s)	263, 1162
NspI	RCATGY	1 cut(s)	1160
NspV	TTCGAA	1 cut(s)	84
OliI	CACNNNNGTG	2 cut(s)	1565, 2216
PaeI	GCATGC	1 cut(s)	1160
PagI	TCATGA	1 cut(s)	1947
PaqCI	CACCTGC	1 cut(s)	678
PceI	AGGCCT	2 cut(s)	1268, 1786
PcsI	WCGNNNNNNNCGW	6 cut(s)	1479, 1559, 1767, 1797, 1812, 2210
PctI	GAATGC	3 cut(s)	139, 834, 1160
PdiI	GCCGGC	2 cut(s)	1111, 2090
PdmI	GAANNNNTTC	1 cut(s)	1460
PfeI	GAWTC	8 cut(s)	101, 876, 890, 998, 1010, 1118, 2194, 2304
PfoI	TCCNGGA	2 cut(s)	1148, 1654
PinAI	ACCGGT	1 cut(s)	167
PkrI	GCNGC	6 cut(s)	526, 928, 968, 1450, 1591, 2235
PleI	GAGTC	8 cut(s)	31, 766, 910, 1024, 1327, 1766, 1852, 1883
PmaCI	CACGTG	1 cut(s)	2214
PmlI	CACGTG	1 cut(s)	2214
PpsI	GAGTC	8 cut(s)	31, 766, 910, 1024, 1327, 1766, 1852, 1883
Ppu21I	YACGTR	1 cut(s)	2214
PpuMI	RGGWCCY	1 cut(s)	54
PshBI	ATTAAT	1 cut(s)	1598
Psp124BI	GAGCTC	2 cut(s)	627, 1940
Psp5II	RGGWCCY	1 cut(s)	54
Psp6I	CCWGG	3 cut(s)	1148, 1538, 1896
PspCI	CACGTG	1 cut(s)	2214
PspFI	CCCAGC	1 cut(s)	1579
PspGI	CCWGG	3 cut(s)	1148, 1538, 1896
PspPI	GGNCC	5 cut(s)	54, 185, 1666, 1767, 2320
PspPPI	RGGWCCY	1 cut(s)	54
PsrI	GAACNNNNNNTAC	2 cut(s)	2276, 2308
PstI	CTGCAG	1 cut(s)	529
PstNI	CAGNNNCTG	2 cut(s)	929, 1411
PsuI	RGATCY	4 cut(s)	76, 214, 607, 736
PvuII	CAGCTG	2 cut(s)	929, 1312
RruI	TCGCGA	1 cut(s)	1797
RsaI	GTAC	9 cut(s)	166, 1481, 1685, 1718, 1919, 2057, 2345, 2378, 2410
RsaNI	GTAC	9 cut(s)	165, 1480, 1684, 1717, 1918, 2056, 2344, 2377, 2409
RseI	CAYNNNNRTG	2 cut(s)	1565, 2216
Rsr2I	CGGWCCG	1 cut(s)	1767
RsrII	CGGWCCG	1 cut(s)	1767
SacI	GAGCTC	2 cut(s)	627, 1940
SalI	GTCGAC	1 cut(s)	2038
SaqAI	TTAA	4 cut(s)	32, 630, 1598, 2349
SatI	GCNGC	6 cut(s)	525, 927, 967, 1449, 1590, 2234
Sau96I	GGNCC	5 cut(s)	54, 185, 1666, 1767, 2320
SchI	GAGTC	8 cut(s)	31, 767, 911, 1025, 1328, 1766, 1853, 1883
SduI	GDGCHC	5 cut(s)	627, 1752, 1940, 1991, 2213
SfaNI	GCATC	7 cut(s)	248, 270, 442, 550, 674, 1212, 1488
SfcI	CTRYAG	2 cut(s)	525, 921
SfuI	TTCGAA	1 cut(s)	84
SinI	GGWCC	5 cut(s)	54, 185, 1666, 1767, 2320
SmaI	CCCGGG	2 cut(s)	1051, 2325
SmiMI	CAYNNNNRTG	2 cut(s)	1565, 2216
SmlI	CTYRAG	3 cut(s)	191, 629, 705
SmoI	CTYRAG	3 cut(s)	191, 629, 705
SphI	GCATGC	1 cut(s)	1160
Sse9I	AATT	8 cut(s)	545, 791, 869, 1043, 1286, 1414, 1428, 1618
SseBI	AGGCCT	2 cut(s)	1268, 1786
SspMI	CTAG	2 cut(s)	884, 1190
SstI	GAGCTC	2 cut(s)	627, 1940
StuI	AGGCCT	2 cut(s)	1268, 1786
StyI	CCWWGG	2 cut(s)	60, 849
TaaI	ACNGT	6 cut(s)	164, 700, 953, 1461, 1567, 1793
TaiI	ACGT	7 cut(s)	1215, 1823, 1929, 2084, 2216, 2318, 2378
TaqII	GACCGA	1 cut(s)	2277
TasI	AATT	8 cut(s)	545, 791, 869, 1043, 1286, 1414, 1428, 1618
TatI	WGTACW	3 cut(s)	1716, 1917, 2343
TauI	GCSGC	2 cut(s)	1451, 1592
TfiI	GAWTC	8 cut(s)	101, 876, 890, 998, 1010, 1118, 2194, 2304
Tru1I	TTAA	4 cut(s)	32, 630, 1598, 2349
Tru9I	TTAA	4 cut(s)	32, 630, 1598, 2349
TscAI	CASTG	3 cut(s)	976, 1232, 1572
TseFI	GTSAC	3 cut(s)	70, 551, 2225
TseI	GCWGC	4 cut(s)	524, 926, 966, 2233
Tsp45I	GTSAC	3 cut(s)	70, 551, 2225
TspDTI	ATGAA	9 cut(s)	258, 309, 384, 531, 1182, 1706, 1936, 1967, 2186
TspGWI	ACGGA	5 cut(s)	89, 524, 760, 1568, 2057
TspMI	CCCGGG	2 cut(s)	1049, 2323
TspRI	CASTG	3 cut(s)	976, 1232, 1572
Vha464I	CTTAAG	1 cut(s)	629
VpaK11BI	GGWCC	5 cut(s)	54, 185, 1666, 1767, 2320
VspI	ATTAAT	1 cut(s)	1598
XapI	RAATTY	5 cut(s)	791, 869, 1043, 1414, 1618
XbaI	TCTAGA	1 cut(s)	883
XceI	RCATGY	1 cut(s)	1160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	856
XmaI	CCCGGG	2 cut(s)	1049, 2323
XmiI	GTMKAC	2 cut(s)	1137, 2039
XmnI	GAANNNNTTC	1 cut(s)	1460
XspI	CTAG	2 cut(s)	884, 1190
Zsp2I	ATGCAT	2 cut(s)	263, 1162

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.