Rh2DG694600

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007697
Physical Location & Seq
Reverse (-)
91072183 .. 91075396
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG694600.1

Sequence Viewer

Length: 2430 bp
ATGGATGAGGCTACCAACACCCCTAAGGCATTAAAGACTCCTAAGGCTTTAGGGACCCCCAAGGGCAATGTGACGGATCCATTCGAAAATCAAAAGGAAGATTCCGAGGAGAACAAAAAGCAGGGACAAGAGAATGCTGATCAGAAGGAGGAAGAGAAGGACAGTACCGGTGGCGAACAAAGCGGTCCCCTTGAGCCGGTTGAGATACCGGAAGATCCCCATCCCACCCTCGAAAAGCTTTGTGATGACATTGAGCGATGCATCTCTTCATTGCCTACACCTAACGAGAAAGATGAAAACCAACAAGAAGAGAAGGATAATGCAAATGATGAGGCAAAAGATACAAAAGGTGATGAGAAGGATGGGAAGGATACAAAAGATGACAAGGAAGAAAAGGATGTAAAAGATGAGAAGGGTGAAAAGGAAGAAGAGAAGGGTGAGAAAGATGCAGAGGAGGGTGAAAAGAATGACAAGGAAGAAAAGGATGTGAAAGATGAGAATAAGCAGGATGATGAAAAGGCTGCAGATCAGGATGAGAAAGAATTAGGTGACAAAGATGCAAAGGATGAGAAAGAGGCTGAAAAAGTCGTCGAAAAAGAAAAGGATCTCGTTCCAGACGAGCTCCTTAAGCTTATTGGCAAGTTATTGACTTGGGTAGATGATATCTGTTGCGAAGGCAGATGCAGGTGGGGAACGGTGCCTCAAGACGACGAGGTGTTCTTGGAGGCTGTGGATCTGATGGCCAAGATGAGGGAGTCTCTCTTGTCCGTCTGTTCTATGGGATTGAAATTCCAAGAAAGGCACGGTGGTTTGATCAACCGCCTAAGTAGCATTCAGCACCGAGCCATGGCCTATCTGGAAGAAGAATTCAGAATCCTTCTAGAAGAATCCAAAACCGAGTCTGATCATCATCACTCTACAGCAGCTGATCACAACCATAACAGTAACAAAGACAGCAGCAGTGGTAACAAAGGAAAGAACGATCAAGAATCACAACCGGAATCCGAGTCAGAATCAAATAAAGAAGGGGTTGTTGAATTTCCCGGGTTCCCTGAGGAGGTCGTGTCCAACCTGAATAAAATAGCCAACAAGATGATCTCCGCCGGCTACGAATCCGAGTGTTGCGAGGTCTACATCATCTCCAGGAGGCATGCATTCGATGAAAATCTCCACAAGCTCGGCCTAGAGAAGCACAGCATCGACGACGTGCAGAAGATGCACTGGGAAGCGTTGGAGAGAGAGATCATGTTGTGGGTCAAGGCATTCAAGCAATGCTCGCAATTCTACTTTTCCGGTGAGCGCCAGCTGGCAGAGTCGGTGTTTCCGGAGCCGGTGCTTTCCGAGAAGACGCCGATATGGTCGTGTCTGTTCAGCAATCTGAGCAGAGGAGTGATGATACAGCTCCTGAATTTTGCCGAAGGAATCGCCATGTCGAAAAGAGCGGCGGAGAAACTGTTCAAGACGCTCGACATGTACGAGGCGGTAAAGGAGATGCTCCCCAAGATAGACGAGCTTTTTCCGGGGGAGTGCGCCAGGGAGCTGAAGACGGAGACCACGACGGTGCTGAACCGGCTGGGCGAGGCGGCTATATTAATCTTCTGTGATCTGGAGAATTCCATCAAGGCCGAAACGGGTCGGAACCCGGTTCCGGGCGGGGCGGTCCACCCGTTGACCCGGTACACGATGAACTACCTGAGATACGCGTGCGAGTACAAGGAGACGCTGGAGCTGGTGTTCAAGGAGCACACCAAGATCGAGCGGTCCGACTCGACGAGCAGGCCTGACGGTCGCGACGATTACGACGCGAGCGACACGTCGTTCGAGATACCCGACGACGAGTCGCCGTTCGCGGTGCAACTGGCGCGGGTGATGGATTTGTTGGACTCGAACCTGGAGGGGAAGGCGAAGCTGTACAAGGACGTGGCGTTGAGCTCGATTTTCATGATGAACAACGGGCGCTACATCTTGCAGAAGATCAAGGGCTCTCCGGAGATCAACGCCTGCATGGGCGACAAGTGGTGCCGGAAGAGGTCGACGGAGCTGAGGCAGTACCACAAGAACTACCAGAGAGAGACGTGGAGCCGGCTGTTGAGTTTTTTGAGCCACGAGGGGCTGAGCAACCACGGCAAAGTGAGCAAGCCTGTGCTGAAAGAGAGGTTCAAGAGCTTCAACGCTATGTTTGATGAGATTCATAAGACGCAGAGCACGTGGGTGGTGAGTGACGAGCAGCTTCAGTCGGAGCTTAGGGTTTCCATATCGGCGGTGGTCATTCCGGCGTACCGGTCGTTCTTGGGGAGATTCTCGCAGACGTTGGACCCGGGAAGGCAAACAGAGAAGTACATTAAGTTTCAGCCGGAAGATATAGAGACGTACATTGATGAGCTATTTGATGGCAACCCCAGTACCTCCATATTTAGGAAAAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

809

Amino Acids

92.35

Weight (kDa)

4.86

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 418 - 794 8.7e-126 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 675
Acc36I ACCTGC 1 cut(s) 675
AccB1I GGYRCC 2 cut(s) 697, 2019
AccBSI CCGCTC 2 cut(s) 1442, 1759
AccI GTMKAC 2 cut(s) 1131, 2033
AccII CGCG 5 cut(s) 1703, 1791, 1805, 1850, 1864
AccIII TCCGGA 2 cut(s) 1324, 1987
AclWI GGATC 5 cut(s) 71, 84, 209, 612, 741
AcoI YGGCCR 1 cut(s) 741
AcsI RAATTY 5 cut(s) 788, 866, 1037, 1408, 1612
AcuI CTGAAG 2 cut(s) 1562, 2216
AcvI CACGTG 1 cut(s) 2208
AcyI GRCGYC 1 cut(s) 1349
AfiI CCNNNNNNNGG 7 cut(s) 196, 750, 847, 1057, 1648, 1649, 2415
AflII CTTAAG 1 cut(s) 626
AflIII ACRYGT 3 cut(s) 1470, 1701, 1812
AgeI ACCGGT 2 cut(s) 167, 2280
AgsI TTSAA 7 cut(s) 787, 1037, 1267, 1459, 1738, 2161, 2170
AhdI GACNNNNNGTC 1 cut(s) 1837
AjiI CACGTC 4 cut(s) 1207, 1815, 1921, 2076
AjnI CCWGG 3 cut(s) 1142, 1532, 1890
AleI CACNNNNGTG 2 cut(s) 1559, 2210
AloI GAACNNNNNNTCC 4 cut(s) 1439, 1471, 1718, 1750
Alw21I GWGCWC 4 cut(s) 624, 1746, 1934, 2207
Alw26I GTCTC 5 cut(s) 762, 1544, 1712, 2066, 2360
AlwI GGATC 5 cut(s) 71, 84, 209, 612, 741
AlwNI CAGNNNCTG 2 cut(s) 926, 1405
Ama87I CYCGRG 2 cut(s) 1043, 2317
Aor13HI TCCGGA 2 cut(s) 1324, 1987
AoxI GGCC 5 cut(s) 741, 849, 1180, 1623, 1778
ApeKI GCWGC 4 cut(s) 521, 923, 957, 2227
ApoI RAATTY 5 cut(s) 788, 866, 1037, 1408, 1612
ArsI GACNNNNNNTTYG 2 cut(s) 1802, 1834
AseI ATTAAT 1 cut(s) 1592
AsiGI ACCGGT 2 cut(s) 167, 2280
Asp700I GAANNNNTTC 1 cut(s) 1454
AspLEI GCGC 4 cut(s) 1302, 1532, 1864, 1959
AspS9I GGNCC 5 cut(s) 54, 185, 1660, 1761, 2314
AsuC2I CCSGG 8 cut(s) 1044, 1045, 1521, 1643, 1650, 1675, 2318, 2319
AsuHPI GGTGA 8 cut(s) 362, 428, 449, 470, 560, 1307, 1879, 2227
AsuII TTCGAA 1 cut(s) 84
AvaI CYCGRG 2 cut(s) 1043, 2317
AvaII GGWCC 5 cut(s) 54, 185, 1660, 1761, 2314
AxyI CCTNAGG 3 cut(s) 24, 42, 1053
BaeI ACNNNNGTAYC 2 cut(s) 2386, 2419
BalI TGGCCA 1 cut(s) 743
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 2 cut(s) 697, 2019
BanII GRGCYC 3 cut(s) 624, 1934, 1985
BauI CACGAG 1 cut(s) 2105
BbrPI CACGTG 1 cut(s) 2208
BbsI GAAGAC 2 cut(s) 1352, 1550
Bbv12I GWGCWC 4 cut(s) 624, 1746, 1934, 2207
BbvCI CCTCAGC 1 cut(s) 2042
BbvI GCAGC 4 cut(s) 508, 935, 969, 2239
BccI CCATC 6 cut(s) 228, 356, 733, 1625, 1864, 2384
BceAI ACGGC 2 cut(s) 1828, 2140
BciT130I CCWGG 3 cut(s) 1144, 1534, 1892
BciVI GTATCC 1 cut(s) 364
BclI TGATCA 4 cut(s) 139, 813, 904, 928
BcnI CCSGG 8 cut(s) 1044, 1045, 1521, 1643, 1650, 1675, 2318, 2319
BcoDI GTCTC 5 cut(s) 762, 1544, 1712, 2066, 2360
BfaI CTAG 2 cut(s) 881, 1184
BfmI CTRYAG 2 cut(s) 522, 918
BfoI RGCGCY 2 cut(s) 1303, 1960
BfrI CTTAAG 1 cut(s) 626
BfuAI ACCTGC 1 cut(s) 675
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 6 cut(s) 522, 924, 958, 1443, 1584, 2228
BlpI GCTNAGC 1 cut(s) 2114
BlsI GCNGC 6 cut(s) 523, 925, 959, 1444, 1585, 2229
Bme18I GGWCC 5 cut(s) 54, 185, 1660, 1761, 2314
BmeRI GACNNNNNGTC 1 cut(s) 1837
BmeT110I CYCGRG 2 cut(s) 1043, 2317
BmgBI CACGTC 4 cut(s) 1207, 1815, 1921, 2076
BmgT120I GGNCC 5 cut(s) 54, 185, 1660, 1761, 2314
BmrI ACTGGG 2 cut(s) 1231, 2394
BmsI GCATC 8 cut(s) 248, 270, 436, 547, 671, 1206, 1206, 1482
BmuI ACTGGG 2 cut(s) 1231, 2394
BpiI GAAGAC 2 cut(s) 1352, 1550
BplI GAGNNNNNCTC 2 cut(s) 742, 774
BpmI CTGGAG 4 cut(s) 1126, 1628, 1745, 1913
Bpu10I CCTNAGC 2 cut(s) 2042, 2243
Bpu1102I GCTNAGC 1 cut(s) 2114
Bpu14I TTCGAA 1 cut(s) 84
BpuEI CTTGAG 2 cut(s) 212, 687
BpuMI CCSGG 8 cut(s) 1044, 1045, 1521, 1643, 1650, 1675, 2318, 2319
BsaAI YACGTR 1 cut(s) 2208
BsaBI GATNNNNATC 3 cut(s) 219, 909, 1164
BsaHI GRCGYC 1 cut(s) 1349
BsaI GGTCTC 1 cut(s) 1544
BsaJI CCNNGG 8 cut(s) 60, 105, 846, 1043, 1520, 1533, 2122, 2317
BsaWI WCCGGW 7 cut(s) 167, 208, 997, 1292, 1324, 1987, 2280
BsaXI ACNNNNNCTCC 8 cut(s) 1049, 1079, 1124, 1154, 1439, 1469, 1718, 1748
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 750, 847, 1057, 1648, 1649, 2415
Bse118I RCCGGY 7 cut(s) 167, 196, 1103, 1330, 1569, 2082, 2280
Bse1I ACTGG 3 cut(s) 1226, 1863, 2400
Bse21I CCTNAGG 3 cut(s) 24, 42, 1053
Bse3DI GCAATG 3 cut(s) 73, 269, 1277
Bse8I GATNNNNATC 3 cut(s) 219, 909, 1164
BseAI TCCGGA 2 cut(s) 1324, 1987
BseBI CCWGG 3 cut(s) 1144, 1534, 1892
BseDI CCNNGG 8 cut(s) 60, 105, 846, 1043, 1520, 1533, 2122, 2317
BseGI GGATG 8 cut(s) 10, 220, 367, 403, 490, 514, 538, 571
BseJI GATNNNNATC 3 cut(s) 219, 909, 1164
BseLI CCNNNNNNNGG 7 cut(s) 196, 750, 847, 1057, 1648, 1649, 2415
BseMI GCAATG 3 cut(s) 73, 269, 1277
BseMII CTCAG 5 cut(s) 1044, 1370, 1685, 2033, 2105
BseNI ACTGG 3 cut(s) 1226, 1863, 2400
BseRI GAGGAG 4 cut(s) 122, 467, 1070, 1401
BseXI GCAGC 4 cut(s) 508, 935, 969, 2239
BseYI CCCAGC 1 cut(s) 1573
BsgI GTGCAG 1 cut(s) 1229
Bsh1236I CGCG 5 cut(s) 1703, 1791, 1805, 1850, 1864
Bsh1285I CGRYCG 2 cut(s) 1789, 2285
BshFI GGCC 5 cut(s) 743, 851, 1182, 1625, 1780
BshNI GGYRCC 2 cut(s) 697, 2019
BshTI ACCGGT 2 cut(s) 167, 2280
BsiEI CGRYCG 2 cut(s) 1789, 2285
BsiHKAI GWGCWC 4 cut(s) 624, 1746, 1934, 2207
BsiHKCI CYCGRG 2 cut(s) 1043, 2317
BslFI GGGAC 3 cut(s) 67, 138, 171
BslI CCNNNNNNNGG 7 cut(s) 196, 750, 847, 1057, 1648, 1649, 2415
BsmAI GTCTC 5 cut(s) 762, 1544, 1712, 2066, 2360
BsmBI CGTCTC 3 cut(s) 1712, 2066, 2360
BsmFI GGGAC 3 cut(s) 67, 138, 171
BsmI GAATGC 4 cut(s) 139, 831, 1154, 1262
BsnI GGCC 5 cut(s) 743, 851, 1182, 1625, 1780
Bso31I GGTCTC 1 cut(s) 1544
BsoBI CYCGRG 2 cut(s) 1043, 2317
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 5 cut(s) 624, 1746, 1934, 1985, 2207
Bsp13I TCCGGA 2 cut(s) 1324, 1987
Bsp1407I TGTACA 1 cut(s) 1911
Bsp1720I GCTNAGC 1 cut(s) 2114
Bsp19I CCATGG 1 cut(s) 846
Bsp68I TCGCGA 1 cut(s) 1791
BspANI GGCC 5 cut(s) 743, 851, 1182, 1625, 1780
BspCNI CTCAG 5 cut(s) 1045, 1371, 1686, 2034, 2106
BspEI TCCGGA 2 cut(s) 1324, 1987
BspFNI CGCG 5 cut(s) 1703, 1791, 1805, 1850, 1864
BspHI TCATGA 1 cut(s) 1941
BspMAI CTGCAG 1 cut(s) 526
BspMI ACCTGC 1 cut(s) 675
BspPI GGATC 5 cut(s) 71, 84, 209, 612, 741
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 2 cut(s) 697, 2019
BspTI CTTAAG 1 cut(s) 626
BspTNI GGTCTC 1 cut(s) 1544
BsrBI CCGCTC 2 cut(s) 1442, 1759
BsrDI GCAATG 3 cut(s) 73, 269, 1277
BsrFI RCCGGY 7 cut(s) 167, 196, 1103, 1330, 1569, 2082, 2280
BsrGI TGTACA 1 cut(s) 1911
BsrI ACTGG 3 cut(s) 1226, 1863, 2400
BssAI RCCGGY 7 cut(s) 167, 196, 1103, 1330, 1569, 2082, 2280
BssECI CCNNGG 8 cut(s) 60, 105, 846, 1043, 1520, 1533, 2122, 2317
BssNI GRCGYC 1 cut(s) 1349
BssSI CACGAG 1 cut(s) 2105
BssT1I CCWWGG 2 cut(s) 60, 846
Bst2BI CACGAG 1 cut(s) 2105
Bst2UI CCWGG 3 cut(s) 1144, 1534, 1892
Bst4CI ACNGT 7 cut(s) 164, 697, 806, 944, 1455, 1561, 1787
Bst6I CTCTTC 5 cut(s) 147, 271, 303, 423, 2021
BstACI GRCGYC 1 cut(s) 1349
BstAFI CTTAAG 1 cut(s) 626
BstAPI GCANNNNNTGC 1 cut(s) 1216
BstAUI TGTACA 1 cut(s) 1911
BstBAI YACGTR 1 cut(s) 2208
BstBI TTCGAA 1 cut(s) 84
BstDEI CTNAG 9 cut(s) 24, 42, 824, 1053, 1379, 1694, 2042, 2114, 2243
BstDSI CCRYGG 2 cut(s) 846, 2122
BstF5I GGATG 8 cut(s) 10, 220, 367, 403, 490, 514, 538, 571
BstFNI CGCG 5 cut(s) 1703, 1791, 1805, 1850, 1864
BstH2I RGCGCY 2 cut(s) 1303, 1960
BstHHI GCGC 4 cut(s) 1302, 1532, 1864, 1959
BstMAI GTCTC 5 cut(s) 762, 1544, 1712, 2066, 2360
BstMCI CGRYCG 2 cut(s) 1789, 2285
BstNI CCWGG 3 cut(s) 1144, 1534, 1892
BstNSI RCATGY 2 cut(s) 1154, 1474
BstSFI CTRYAG 2 cut(s) 522, 918
BstUI CGCG 5 cut(s) 1703, 1791, 1805, 1850, 1864
BstV1I GCAGC 4 cut(s) 508, 935, 969, 2239
BstV2I GAAGAC 2 cut(s) 1352, 1550
BstX2I RGATCY 4 cut(s) 76, 214, 604, 733
BstYI RGATCY 4 cut(s) 76, 214, 604, 733
Bsu36I CCTNAGG 3 cut(s) 24, 42, 1053
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 5 cut(s) 743, 851, 1182, 1625, 1780
BtgI CCRYGG 2 cut(s) 846, 2122
BtgZI GCGATG 1 cut(s) 271
BtrI CACGTC 4 cut(s) 1207, 1815, 1921, 2076
BtsCI GGATG 8 cut(s) 10, 220, 367, 403, 490, 514, 538, 571
BtsI GCAGTG 1 cut(s) 967
BtsIMutI CAGTG 2 cut(s) 967, 1219
BtuMI TCGCGA 1 cut(s) 1791
BveI ACCTGC 1 cut(s) 675
CaiI CAGNNNCTG 2 cut(s) 926, 1405
CciI TCATGA 1 cut(s) 1941
CfoI GCGC 4 cut(s) 1302, 1532, 1864, 1959
Cfr10I RCCGGY 7 cut(s) 167, 196, 1103, 1330, 1569, 2082, 2280
Cfr13I GGNCC 5 cut(s) 54, 185, 1660, 1761, 2314
Cfr9I CCCGGG 2 cut(s) 1043, 2317
CpoI CGGWCCG 1 cut(s) 1761
CseI GACGC 5 cut(s) 1357, 1471, 1729, 1811, 2206
CspAI ACCGGT 2 cut(s) 167, 2280
CspI CGGWCCG 1 cut(s) 1761
CviAII CATG 7 cut(s) 847, 1151, 1246, 1429, 1471, 1942, 2005
DdeI CTNAG 9 cut(s) 24, 42, 824, 1053, 1379, 1694, 2042, 2114, 2243
DriI GACNNNNNGTC 1 cut(s) 1837
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 5 cut(s) 147, 271, 303, 423, 2021
Eam1105I GACNNNNNGTC 1 cut(s) 1837
EarI CTCTTC 5 cut(s) 147, 271, 303, 423, 2021
EciI GGCGGA 2 cut(s) 1090, 1460
Ecl136II GAGCTC 2 cut(s) 622, 1932
Eco130I CCWWGG 2 cut(s) 60, 846
Eco147I AGGCCT 1 cut(s) 1780
Eco24I GRGCYC 3 cut(s) 624, 1934, 1985
Eco31I GGTCTC 1 cut(s) 1544
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 5 cut(s) 54, 185, 1660, 1761, 2314
Eco53kI GAGCTC 2 cut(s) 622, 1932
Eco57I CTGAAG 2 cut(s) 1562, 2216
Eco72I CACGTG 1 cut(s) 2208
Eco81I CCTNAGG 3 cut(s) 24, 42, 1053
Eco88I CYCGRG 2 cut(s) 1043, 2317
EcoICRI GAGCTC 2 cut(s) 622, 1932
EcoO109I RGGNCCY 1 cut(s) 54
EcoRI GAATTC 2 cut(s) 866, 1612
EcoRII CCWGG 3 cut(s) 1142, 1532, 1890
EcoRV GATATC 1 cut(s) 664
EcoT14I CCWWGG 2 cut(s) 60, 846
EcoT22I ATGCAT 2 cut(s) 263, 1156
EcoT38I GRGCYC 3 cut(s) 624, 1934, 1985
ErhI CCWWGG 2 cut(s) 60, 846
Esp3I CGTCTC 3 cut(s) 1712, 2066, 2360
FaeI CATG 7 cut(s) 850, 1154, 1249, 1432, 1474, 1945, 2008
FaqI GGGAC 3 cut(s) 67, 138, 171
FatI CATG 7 cut(s) 846, 1150, 1245, 1428, 1470, 1941, 2004
FauI CCCGC 2 cut(s) 1646, 1857
FbaI TGATCA 4 cut(s) 139, 813, 904, 928
FblI GTMKAC 2 cut(s) 1131, 2033
Fnu4HI GCNGC 6 cut(s) 522, 924, 958, 1443, 1584, 2228
FokI GGATG 8 cut(s) 17, 207, 374, 410, 497, 521, 545, 578
FriOI GRGCYC 3 cut(s) 624, 1934, 1985
Fsp4HI GCNGC 6 cut(s) 522, 924, 958, 1443, 1584, 2228
FspBI CTAG 2 cut(s) 881, 1184
GlaI GCGC 4 cut(s) 1301, 1531, 1863, 1958
GluI GCNGC 6 cut(s) 522, 924, 958, 1443, 1584, 2228
GsaI CCCAGC 1 cut(s) 1577
GsuI CTGGAG 4 cut(s) 1126, 1628, 1745, 1913
HaeII RGCGCY 2 cut(s) 1303, 1960
HaeIII GGCC 5 cut(s) 743, 851, 1182, 1625, 1780
HgaI GACGC 5 cut(s) 1357, 1471, 1729, 1811, 2206
HhaI GCGC 4 cut(s) 1302, 1532, 1864, 1959
Hin1I GRCGYC 1 cut(s) 1349
Hin1II CATG 7 cut(s) 850, 1154, 1249, 1432, 1474, 1945, 2008
Hin6I GCGC 4 cut(s) 1300, 1530, 1862, 1957
HinP1I GCGC 4 cut(s) 1300, 1530, 1862, 1957
HincII GTYRAC 2 cut(s) 1671, 2034
HindII GTYRAC 2 cut(s) 1671, 2034
HindIII AAGCTT 2 cut(s) 236, 629
HphI GGTGA 8 cut(s) 362, 428, 449, 470, 560, 1307, 1879, 2227
Hpy166II GTNNAC 5 cut(s) 1132, 1663, 1671, 1680, 2034
Hpy8I GTNNAC 5 cut(s) 1132, 1663, 1671, 1680, 2034
HpyCH4III ACNGT 7 cut(s) 164, 697, 806, 944, 1455, 1561, 1787
HpyCH4IV ACGT 7 cut(s) 1206, 1814, 1920, 2075, 2207, 2309, 2369
HpyF3I CTNAG 9 cut(s) 24, 42, 824, 1053, 1379, 1694, 2042, 2114, 2243
HpySE526I ACGT 7 cut(s) 1206, 1814, 1920, 2075, 2207, 2309, 2369
Hsp92I GRCGYC 1 cut(s) 1349
Hsp92II CATG 7 cut(s) 850, 1154, 1249, 1432, 1474, 1945, 2008
HspAI GCGC 4 cut(s) 1300, 1530, 1862, 1957
KflI GGGWCCC 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 1324, 1987
KroI GCCGGC 2 cut(s) 1103, 2082
KroNI GCCGGC 2 cut(s) 1105, 2084
Ksp22I TGATCA 4 cut(s) 139, 813, 904, 928
Lsp1109I GCAGC 4 cut(s) 508, 935, 969, 2239
LweI GCATC 8 cut(s) 248, 270, 436, 547, 671, 1206, 1206, 1482
MaeI CTAG 2 cut(s) 881, 1184
MaeII ACGT 7 cut(s) 1206, 1814, 1920, 2075, 2207, 2309, 2369
MaeIII GTNAC 5 cut(s) 70, 548, 944, 965, 2219
MbiI CCGCTC 2 cut(s) 1442, 1759
MflI RGATCY 4 cut(s) 76, 214, 604, 733
MhlI GDGCHC 5 cut(s) 624, 1746, 1934, 1985, 2207
MlsI TGGCCA 1 cut(s) 743
MluCI AATT 7 cut(s) 542, 788, 866, 1037, 1280, 1408, 1612
MluI ACGCGT 1 cut(s) 1701
MluNI TGGCCA 1 cut(s) 743
MlyI GAGTC 8 cut(s) 31, 764, 908, 1016, 1322, 1760, 1847, 1877
MmeI TCCRAC 7 cut(s) 1092, 1212, 1616, 1788, 1860, 2217, 2292
Mox20I TGGCCA 1 cut(s) 743
Mph1103I ATGCAT 2 cut(s) 263, 1156
MroI TCCGGA 2 cut(s) 1324, 1987
MroNI GCCGGC 2 cut(s) 1103, 2082
MroXI GAANNNNTTC 1 cut(s) 1454
MscI TGGCCA 1 cut(s) 743
MseI TTAA 4 cut(s) 32, 627, 1592, 2343
MslI CAYNNNNRTG 2 cut(s) 1559, 2210
Msp20I TGGCCA 1 cut(s) 743
MspA1I CMGCKG 2 cut(s) 926, 1306
MspCI CTTAAG 1 cut(s) 626
Mva1269I GAATGC 4 cut(s) 139, 831, 1154, 1262
MvaI CCWGG 3 cut(s) 1144, 1534, 1892
MvnI CGCG 5 cut(s) 1703, 1791, 1805, 1850, 1864
NaeI GCCGGC 2 cut(s) 1105, 2084
NciI CCSGG 8 cut(s) 1044, 1045, 1521, 1643, 1650, 1675, 2318, 2319
NcoI CCATGG 1 cut(s) 846
NgoMIV GCCGGC 2 cut(s) 1103, 2082
NlaIII CATG 7 cut(s) 850, 1154, 1249, 1432, 1474, 1945, 2008
NmeAIII GCCGAG 1 cut(s) 1158
NmuCI GTSAC 3 cut(s) 70, 548, 2219
NruI TCGCGA 1 cut(s) 1791
NsiI ATGCAT 2 cut(s) 263, 1156
NspI RCATGY 2 cut(s) 1154, 1474
NspV TTCGAA 1 cut(s) 84
OliI CACNNNNGTG 2 cut(s) 1559, 2210
PaeI GCATGC 1 cut(s) 1154
PagI TCATGA 1 cut(s) 1941
PaqCI CACCTGC 1 cut(s) 675
PceI AGGCCT 1 cut(s) 1780
PciI ACATGT 1 cut(s) 1470
PcsI WCGNNNNNNNCGW 8 cut(s) 615, 1473, 1553, 1761, 1791, 1806, 1827, 2204
PctI GAATGC 4 cut(s) 139, 831, 1154, 1262
PdiI GCCGGC 2 cut(s) 1105, 2084
PdmI GAANNNNTTC 1 cut(s) 1454
PfoI TCCNGGA 1 cut(s) 1142
PinAI ACCGGT 2 cut(s) 167, 2280
PkrI GCNGC 6 cut(s) 523, 925, 959, 1444, 1585, 2229
PleI GAGTC 8 cut(s) 31, 763, 907, 1015, 1321, 1760, 1846, 1877
PmaCI CACGTG 1 cut(s) 2208
PmlI CACGTG 1 cut(s) 2208
PpsI GAGTC 8 cut(s) 31, 763, 907, 1015, 1321, 1760, 1846, 1877
Ppu21I YACGTR 1 cut(s) 2208
PpuMI RGGWCCY 1 cut(s) 54
PscI ACATGT 1 cut(s) 1470
PshBI ATTAAT 1 cut(s) 1592
Psp124BI GAGCTC 2 cut(s) 624, 1934
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 3 cut(s) 1142, 1532, 1890
PspCI CACGTG 1 cut(s) 2208
PspFI CCCAGC 1 cut(s) 1573
PspGI CCWGG 3 cut(s) 1142, 1532, 1890
PspPI GGNCC 5 cut(s) 54, 185, 1660, 1761, 2314
PspPPI RGGWCCY 1 cut(s) 54
PsrI GAACNNNNNNTAC 2 cut(s) 2270, 2302
PstI CTGCAG 1 cut(s) 526
PstNI CAGNNNCTG 2 cut(s) 926, 1405
PsuI RGATCY 4 cut(s) 76, 214, 604, 733
PvuII CAGCTG 2 cut(s) 926, 1306
RruI TCGCGA 1 cut(s) 1791
RseI CAYNNNNRTG 2 cut(s) 1559, 2210
Rsr2I CGGWCCG 1 cut(s) 1761
RsrII CGGWCCG 1 cut(s) 1761
SacI GAGCTC 2 cut(s) 624, 1934
SalI GTCGAC 1 cut(s) 2032
SaqAI TTAA 4 cut(s) 32, 627, 1592, 2343
SatI GCNGC 6 cut(s) 522, 924, 958, 1443, 1584, 2228
Sau96I GGNCC 5 cut(s) 54, 185, 1660, 1761, 2314
SchI GAGTC 8 cut(s) 31, 764, 908, 1016, 1322, 1760, 1847, 1877
SduI GDGCHC 5 cut(s) 624, 1746, 1934, 1985, 2207
SfaNI GCATC 8 cut(s) 248, 270, 436, 547, 671, 1206, 1206, 1482
SfcI CTRYAG 2 cut(s) 522, 918
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 5 cut(s) 54, 185, 1660, 1761, 2314
SmaI CCCGGG 2 cut(s) 1045, 2319
SmiMI CAYNNNNRTG 2 cut(s) 1559, 2210
SmlI CTYRAG 3 cut(s) 191, 626, 702
SmoI CTYRAG 3 cut(s) 191, 626, 702
SphI GCATGC 1 cut(s) 1154
Sse9I AATT 7 cut(s) 542, 788, 866, 1037, 1280, 1408, 1612
SseBI AGGCCT 1 cut(s) 1780
SspMI CTAG 2 cut(s) 881, 1184
SstI GAGCTC 2 cut(s) 624, 1934
StuI AGGCCT 1 cut(s) 1780
StyI CCWWGG 2 cut(s) 60, 846
TaaI ACNGT 7 cut(s) 164, 697, 806, 944, 1455, 1561, 1787
TaiI ACGT 7 cut(s) 1209, 1817, 1923, 2078, 2210, 2312, 2372
TasI AATT 7 cut(s) 542, 788, 866, 1037, 1280, 1408, 1612
TatI WGTACW 3 cut(s) 1710, 1911, 2337
TauI GCSGC 2 cut(s) 1445, 1586
Tru1I TTAA 4 cut(s) 32, 627, 1592, 2343
Tru9I TTAA 4 cut(s) 32, 627, 1592, 2343
TscAI CASTG 2 cut(s) 967, 1226
TseFI GTSAC 3 cut(s) 70, 548, 2219
TseI GCWGC 4 cut(s) 521, 923, 957, 2227
Tsp45I GTSAC 3 cut(s) 70, 548, 2219
TspDTI ATGAA 8 cut(s) 258, 309, 528, 1176, 1700, 1930, 1961, 2180
TspGWI ACGGA 4 cut(s) 89, 757, 1562, 2051
TspMI CCCGGG 2 cut(s) 1043, 2317
TspRI CASTG 2 cut(s) 967, 1226
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 5 cut(s) 54, 185, 1660, 1761, 2314
VspI ATTAAT 1 cut(s) 1592
XapI RAATTY 5 cut(s) 788, 866, 1037, 1408, 1612
XbaI TCTAGA 1 cut(s) 880
XceI RCATGY 2 cut(s) 1154, 1474
XcmI CCANNNNNNNNNTGG 2 cut(s) 853, 2260
XmaI CCCGGG 2 cut(s) 1043, 2317
XmiI GTMKAC 2 cut(s) 1131, 2033
XmnI GAANNNNTTC 1 cut(s) 1454
XspI CTAG 2 cut(s) 881, 1184
Zsp2I ATGCAT 2 cut(s) 263, 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.