RchiOBHm_Chr2g0176101

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007697
Physical Location & Seq
Reverse (-)
88037332 .. 88039776
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54313

Sequence Viewer

Length: 2445 bp
ATGGATGAGGCTACCAACACCCCTAAGGCATTAAAGACTCCTAAGGCTTTAGGGACCCCCAAGGGCAATGTGACGGATCCATTCGAAAATCAAAAGGAAGATTCCGAGGAGAACAAAAAGCAGGGACAAGAGAATGCTGATCAGAAGGAGGAAGAGAAGGACAGTACCGGTGGCGAACACAGCGGTCCCCTTGAGCCTGTTGAGATACCGGAAGATCCCCATCCCACCCTCGAAAAGCTTTCTGATGACATTGAGCGATGCATCTCTTCATTGCCTACACCTAACGAGAAAGATGAAAACCAACAAGAAGAGAAGGAAAATGCAAATGATGAGGCAAAAGATACAAAAGGTGATGAGAATGATGGGAAGGATACAAAAGATGACAAGGAAGAAGAGGATGTAAAAGATGAGAAGGGTGAAAAGGAAGAAGAGAAGGGTGAGAAAGATGCAAAGGAGGGTGAAAAGAATGACAAGGAAGAAAAGGATGTGAAAGATGAGAATAAGGATGATGAAAAGGCTGCAGATCAGGATGAGAAAGAATTAGGTGACAAAGATGCAAAGGATGAGAAAGAGGCTGAAAAAGTCGTCGAAAAAGAAAAGGATCTCGTTCCAGACGAGCTCCTTAAGCTTATTGACAAGTTCTTGACTTGGGTAGATGATATCTGTTGCGAAGGCAGATGCAGGTGGGGAACGGTGCCTCAAGACGACGAGGTGTTCTTGGAGGCTGTGGATCTGATGGCCAAGATGAGGGAGTCTCTCTTGTCGGTCTGTTCTATGGGATTGAAATTCCAAGAAAGGCACGGTGGTTTGATCAACCGCCTAAGTAGCATTCAGCACCGAGCCATGGCCTATCTGGAAGAAGAATTCAGAATCCTTCTAGAAGAATCCAAAACCGAGTCTGATCATCATCACTCTACAGCAGCTGATCACAACCATAACCATAACCATAACCATAACAGTAACAAAGACAGCAGCAGTGGTAACAAAGGAAAGAACGATCAAGAATCACAACCGGAATCCGAGTCAGAATCAAATAAAGAAGGGGTTGTTGAATTTCCCGGGTTCCCTGAGGAGGTCGTGTCCAACCTGAATAAAATAGCCACCAAGATGATCTCCGCCGGCTACGAATCCGAGTGTTGCGAGGTCTACATCATCTCCAGGAGGCATGCATTCGATGAAAATCTCCACAAGCTCGGCCTAGAGAAGCACAGCATCGACGACGTGCAGAAGATGCACTGGGAAGCGTTGGAGAGAGAGATCATGTTGTGGGTCAAGGCATTCAAGCAATGCTCGCAATTCTACTTTTCCGGTGAGCGCCAGCTGGCAGAGTCGGTGTTTCCGGAGCCGGTGCTTTCCGAGCAGACGCCGATATGGTCGTGTCTGTTCAGCAATCTGAGCAGAGGAGTGATGATACAGCTCCTGAATTTCGCCGAAGGAATCGCCATGTCGAAAAGAGCGGCGGAGAAACTGTTCAAGACGCTCGACATGTACGAGGCGGTAAAGGAGATGCTCCCCAAGATAGACGAGCTTTTTCCGGGGGAGTGCGCCAGGGAGCTGAAGACGGAGACCACGACGGTGCTGAACCGGCTGGGCGAGGCGGCGATATTAATCTTCTGTGATCTGGAGAATTCCATCAAGGCCGAAACGGGTCGGAACCCGGTTCCGGGCGGGGCGGTCCACCCGTTGACCCGGTACACGATGAACTACCTGAGATACGCGTGCGAGTACAAGGAGACGCTGGAGCTGGTGTTCAAGGAGCACACCAAGATCGAGCGGTCCGACTCGACGAGCAGGCCTGACGGTCGCGACGATTACGACGCGAGCGACACGTCGTTCGAGATACCCGACGACGAGTCGCCGTTCGCGGTGCAACTGGCGCGGGTGATGGACTTGTTGGACTCGAACCTGGAGGGGAAGGCGAAGCTGTACAAGGACGTGGCGTTGAGCTCGATTTTCATGATGAACAACGGGCGCTACATCTTGCAGAAGATCAAGGGCTCTCCGGAGATCAACGCCTGCATGGGCGACAAGTGGTGCCGGAAGAGGTCGACGGAGCTGAGGCAGTACCACAAGAACTACCAGAGAGAGACGTGGAGCCGGCTGTTGAGTTTTTTGAGCCACGAGGGGCTGAGCAACCACGGCAAAGTGAGCAAGCCTGTGCTGAAAGAGAGGTTCAAGAGCTTCAACGCTATGTTTGATGAGATTCATAAGACGCAGAGCACGTGGGTGGTCAGTGACGAGCAGCTTCAGTCGGAGCTTAGGGTTTCCATATCGGCGGTGGTCATTCCGGCGTACCGGTCGTTCTTGGGGAGATTCTCGCAGACGTTGGACCCGGGAAGGCAAACAGAGAAGTACATTAAGTTTCAGCCGGAAGATATAGAGACGTACATTGATGAGCTATTTGATGGCAACCCCAGTACCTCCATATTTAGGAAAAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

814

Amino Acids

93.05

Weight (kDa)

4.88

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 423 - 799 7.2e-126 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 672
Acc36I ACCTGC 1 cut(s) 672
AccB1I GGYRCC 2 cut(s) 694, 2034
AccBSI CCGCTC 2 cut(s) 1457, 1774
AccI GTMKAC 2 cut(s) 1146, 2048
AccII CGCG 5 cut(s) 1718, 1806, 1820, 1865, 1879
AccIII TCCGGA 2 cut(s) 1339, 2002
AclWI GGATC 5 cut(s) 71, 84, 209, 609, 738
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 5 cut(s) 785, 863, 1052, 1423, 1627
AcuI CTGAAG 2 cut(s) 1577, 2231
AcvI CACGTG 1 cut(s) 2223
AcyI GRCGYC 1 cut(s) 1364
AfiI CCNNNNNNNGG 6 cut(s) 747, 844, 1072, 1663, 1664, 2430
AflII CTTAAG 1 cut(s) 623
AflIII ACRYGT 3 cut(s) 1485, 1716, 1827
AgeI ACCGGT 2 cut(s) 167, 2295
AgsI TTSAA 7 cut(s) 784, 1052, 1282, 1474, 1753, 2176, 2185
AhdI GACNNNNNGTC 1 cut(s) 1852
AjiI CACGTC 4 cut(s) 1222, 1830, 1936, 2091
AjnI CCWGG 3 cut(s) 1157, 1547, 1905
AleI CACNNNNGTG 2 cut(s) 1574, 2225
AloI GAACNNNNNNTCC 4 cut(s) 1454, 1486, 1733, 1765
Alw21I GWGCWC 4 cut(s) 621, 1761, 1949, 2222
Alw26I GTCTC 5 cut(s) 759, 1559, 1727, 2081, 2375
AlwI GGATC 5 cut(s) 71, 84, 209, 609, 738
AlwNI CAGNNNCTG 2 cut(s) 923, 1420
Ama87I CYCGRG 2 cut(s) 1058, 2332
Aor13HI TCCGGA 2 cut(s) 1339, 2002
AoxI GGCC 5 cut(s) 738, 846, 1195, 1638, 1793
ApeKI GCWGC 4 cut(s) 518, 920, 972, 2242
ApoI RAATTY 5 cut(s) 785, 863, 1052, 1423, 1627
ArsI GACNNNNNNTTYG 2 cut(s) 1817, 1849
AseI ATTAAT 1 cut(s) 1607
AsiGI ACCGGT 2 cut(s) 167, 2295
Asp700I GAANNNNTTC 1 cut(s) 1469
AspLEI GCGC 4 cut(s) 1317, 1547, 1879, 1974
AspS9I GGNCC 5 cut(s) 54, 185, 1675, 1776, 2329
AsuC2I CCSGG 8 cut(s) 1059, 1060, 1536, 1658, 1665, 1690, 2333, 2334
AsuHPI GGTGA 7 cut(s) 362, 428, 449, 470, 557, 1322, 1894
AsuII TTCGAA 1 cut(s) 84
AvaI CYCGRG 2 cut(s) 1058, 2332
AvaII GGWCC 5 cut(s) 54, 185, 1675, 1776, 2329
AxyI CCTNAGG 3 cut(s) 24, 42, 1068
BaeI ACNNNNGTAYC 2 cut(s) 2401, 2434
BalI TGGCCA 1 cut(s) 740
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 2 cut(s) 694, 2034
BanII GRGCYC 3 cut(s) 621, 1949, 2000
BauI CACGAG 1 cut(s) 2120
BbrPI CACGTG 1 cut(s) 2223
BbsI GAAGAC 1 cut(s) 1565
Bbv12I GWGCWC 4 cut(s) 621, 1761, 1949, 2222
BbvCI CCTCAGC 1 cut(s) 2057
BbvI GCAGC 4 cut(s) 505, 932, 984, 2254
BccI CCATC 6 cut(s) 228, 356, 730, 1640, 1879, 2399
BceAI ACGGC 2 cut(s) 1843, 2155
BciT130I CCWGG 3 cut(s) 1159, 1549, 1907
BciVI GTATCC 1 cut(s) 364
BclI TGATCA 4 cut(s) 139, 810, 901, 925
BcnI CCSGG 8 cut(s) 1059, 1060, 1536, 1658, 1665, 1690, 2333, 2334
BcoDI GTCTC 5 cut(s) 759, 1559, 1727, 2081, 2375
BfaI CTAG 2 cut(s) 878, 1199
BfmI CTRYAG 2 cut(s) 519, 915
BfoI RGCGCY 2 cut(s) 1318, 1975
BfrI CTTAAG 1 cut(s) 623
BfuAI ACCTGC 1 cut(s) 672
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 6 cut(s) 519, 921, 973, 1458, 1599, 2243
BlpI GCTNAGC 1 cut(s) 2129
BlsI GCNGC 6 cut(s) 520, 922, 974, 1459, 1600, 2244
Bme18I GGWCC 5 cut(s) 54, 185, 1675, 1776, 2329
BmeRI GACNNNNNGTC 1 cut(s) 1852
BmeT110I CYCGRG 2 cut(s) 1058, 2332
BmgBI CACGTC 4 cut(s) 1222, 1830, 1936, 2091
BmgT120I GGNCC 5 cut(s) 54, 185, 1675, 1776, 2329
BmrI ACTGGG 2 cut(s) 1246, 2409
BmsI GCATC 8 cut(s) 248, 270, 436, 544, 668, 1221, 1221, 1497
BmuI ACTGGG 2 cut(s) 1246, 2409
BpiI GAAGAC 1 cut(s) 1565
BplI GAGNNNNNCTC 2 cut(s) 739, 771
BpmI CTGGAG 4 cut(s) 1141, 1643, 1760, 1928
Bpu10I CCTNAGC 2 cut(s) 2057, 2258
Bpu1102I GCTNAGC 1 cut(s) 2129
Bpu14I TTCGAA 1 cut(s) 84
BpuEI CTTGAG 2 cut(s) 212, 684
BpuMI CCSGG 8 cut(s) 1059, 1060, 1536, 1658, 1665, 1690, 2333, 2334
BsaAI YACGTR 1 cut(s) 2223
BsaBI GATNNNNATC 4 cut(s) 219, 906, 1179, 1607
BsaHI GRCGYC 1 cut(s) 1364
BsaI GGTCTC 1 cut(s) 1559
BsaJI CCNNGG 8 cut(s) 60, 105, 843, 1058, 1535, 1548, 2137, 2332
BsaWI WCCGGW 7 cut(s) 167, 208, 1012, 1307, 1339, 2002, 2295
BsaXI ACNNNNNCTCC 8 cut(s) 1064, 1094, 1139, 1169, 1454, 1484, 1733, 1763
Bsc4I CCNNNNNNNGG 6 cut(s) 747, 844, 1072, 1663, 1664, 2430
Bse118I RCCGGY 6 cut(s) 167, 1118, 1345, 1584, 2097, 2295
Bse1I ACTGG 3 cut(s) 1241, 1878, 2415
Bse21I CCTNAGG 3 cut(s) 24, 42, 1068
Bse3DI GCAATG 3 cut(s) 73, 269, 1292
Bse8I GATNNNNATC 4 cut(s) 219, 906, 1179, 1607
BseAI TCCGGA 2 cut(s) 1339, 2002
BseBI CCWGG 3 cut(s) 1159, 1549, 1907
BseDI CCNNGG 8 cut(s) 60, 105, 843, 1058, 1535, 1548, 2137, 2332
BseGI GGATG 7 cut(s) 10, 220, 403, 490, 511, 535, 568
BseJI GATNNNNATC 4 cut(s) 219, 906, 1179, 1607
BseLI CCNNNNNNNGG 6 cut(s) 747, 844, 1072, 1663, 1664, 2430
BseMI GCAATG 3 cut(s) 73, 269, 1292
BseMII CTCAG 5 cut(s) 1059, 1385, 1700, 2048, 2120
BseNI ACTGG 3 cut(s) 1241, 1878, 2415
BseRI GAGGAG 3 cut(s) 122, 1085, 1416
BseXI GCAGC 4 cut(s) 505, 932, 984, 2254
BseYI CCCAGC 1 cut(s) 1588
BsgI GTGCAG 1 cut(s) 1244
Bsh1236I CGCG 5 cut(s) 1718, 1806, 1820, 1865, 1879
Bsh1285I CGRYCG 2 cut(s) 1804, 2300
BshFI GGCC 5 cut(s) 740, 848, 1197, 1640, 1795
BshNI GGYRCC 2 cut(s) 694, 2034
BshTI ACCGGT 2 cut(s) 167, 2295
BsiEI CGRYCG 2 cut(s) 1804, 2300
BsiHKAI GWGCWC 4 cut(s) 621, 1761, 1949, 2222
BsiHKCI CYCGRG 2 cut(s) 1058, 2332
BslFI GGGAC 3 cut(s) 67, 138, 171
BslI CCNNNNNNNGG 6 cut(s) 747, 844, 1072, 1663, 1664, 2430
BsmAI GTCTC 5 cut(s) 759, 1559, 1727, 2081, 2375
BsmBI CGTCTC 3 cut(s) 1727, 2081, 2375
BsmFI GGGAC 3 cut(s) 67, 138, 171
BsmI GAATGC 4 cut(s) 139, 828, 1169, 1277
BsnI GGCC 5 cut(s) 740, 848, 1197, 1640, 1795
Bso31I GGTCTC 1 cut(s) 1559
BsoBI CYCGRG 2 cut(s) 1058, 2332
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 5 cut(s) 621, 1761, 1949, 2000, 2222
Bsp13I TCCGGA 2 cut(s) 1339, 2002
Bsp1407I TGTACA 1 cut(s) 1926
Bsp1720I GCTNAGC 1 cut(s) 2129
Bsp19I CCATGG 1 cut(s) 843
Bsp68I TCGCGA 1 cut(s) 1806
BspANI GGCC 5 cut(s) 740, 848, 1197, 1640, 1795
BspCNI CTCAG 5 cut(s) 1060, 1386, 1701, 2049, 2121
BspEI TCCGGA 2 cut(s) 1339, 2002
BspFNI CGCG 5 cut(s) 1718, 1806, 1820, 1865, 1879
BspHI TCATGA 1 cut(s) 1956
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 1 cut(s) 672
BspPI GGATC 5 cut(s) 71, 84, 209, 609, 738
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 2 cut(s) 694, 2034
BspTI CTTAAG 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 1559
BsrBI CCGCTC 2 cut(s) 1457, 1774
BsrDI GCAATG 3 cut(s) 73, 269, 1292
BsrFI RCCGGY 6 cut(s) 167, 1118, 1345, 1584, 2097, 2295
BsrGI TGTACA 1 cut(s) 1926
BsrI ACTGG 3 cut(s) 1241, 1878, 2415
BssAI RCCGGY 6 cut(s) 167, 1118, 1345, 1584, 2097, 2295
BssECI CCNNGG 8 cut(s) 60, 105, 843, 1058, 1535, 1548, 2137, 2332
BssNI GRCGYC 1 cut(s) 1364
BssSI CACGAG 1 cut(s) 2120
BssT1I CCWWGG 2 cut(s) 60, 843
Bst2BI CACGAG 1 cut(s) 2120
Bst2UI CCWGG 3 cut(s) 1159, 1549, 1907
Bst4CI ACNGT 7 cut(s) 164, 694, 803, 959, 1470, 1576, 1802
Bst6I CTCTTC 6 cut(s) 147, 271, 303, 387, 423, 2036
BstACI GRCGYC 1 cut(s) 1364
BstAFI CTTAAG 1 cut(s) 623
BstAPI GCANNNNNTGC 1 cut(s) 1231
BstAUI TGTACA 1 cut(s) 1926
BstBAI YACGTR 1 cut(s) 2223
BstBI TTCGAA 1 cut(s) 84
BstDEI CTNAG 9 cut(s) 24, 42, 821, 1068, 1394, 1709, 2057, 2129, 2258
BstDSI CCRYGG 2 cut(s) 843, 2137
BstF5I GGATG 7 cut(s) 10, 220, 403, 490, 511, 535, 568
BstFNI CGCG 5 cut(s) 1718, 1806, 1820, 1865, 1879
BstH2I RGCGCY 2 cut(s) 1318, 1975
BstHHI GCGC 4 cut(s) 1317, 1547, 1879, 1974
BstMAI GTCTC 5 cut(s) 759, 1559, 1727, 2081, 2375
BstMCI CGRYCG 2 cut(s) 1804, 2300
BstNI CCWGG 3 cut(s) 1159, 1549, 1907
BstNSI RCATGY 2 cut(s) 1169, 1489
BstSFI CTRYAG 2 cut(s) 519, 915
BstUI CGCG 5 cut(s) 1718, 1806, 1820, 1865, 1879
BstV1I GCAGC 4 cut(s) 505, 932, 984, 2254
BstV2I GAAGAC 1 cut(s) 1565
BstX2I RGATCY 4 cut(s) 76, 214, 601, 730
BstYI RGATCY 4 cut(s) 76, 214, 601, 730
Bsu36I CCTNAGG 3 cut(s) 24, 42, 1068
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 5 cut(s) 740, 848, 1197, 1640, 1795
BtgI CCRYGG 2 cut(s) 843, 2137
BtgZI GCGATG 1 cut(s) 271
BtrI CACGTC 4 cut(s) 1222, 1830, 1936, 2091
BtsCI GGATG 7 cut(s) 10, 220, 403, 490, 511, 535, 568
BtsI GCAGTG 1 cut(s) 982
BtsIMutI CAGTG 3 cut(s) 982, 1234, 2239
BtuMI TCGCGA 1 cut(s) 1806
BveI ACCTGC 1 cut(s) 672
CaiI CAGNNNCTG 2 cut(s) 923, 1420
CciI TCATGA 1 cut(s) 1956
CfoI GCGC 4 cut(s) 1317, 1547, 1879, 1974
Cfr10I RCCGGY 6 cut(s) 167, 1118, 1345, 1584, 2097, 2295
Cfr13I GGNCC 5 cut(s) 54, 185, 1675, 1776, 2329
Cfr9I CCCGGG 2 cut(s) 1058, 2332
CpoI CGGWCCG 1 cut(s) 1776
CseI GACGC 5 cut(s) 1372, 1486, 1744, 1826, 2221
CspAI ACCGGT 2 cut(s) 167, 2295
CspI CGGWCCG 1 cut(s) 1776
CviAII CATG 7 cut(s) 844, 1166, 1261, 1444, 1486, 1957, 2020
DdeI CTNAG 9 cut(s) 24, 42, 821, 1068, 1394, 1709, 2057, 2129, 2258
DriI GACNNNNNGTC 1 cut(s) 1852
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 6 cut(s) 147, 271, 303, 387, 423, 2036
Eam1105I GACNNNNNGTC 1 cut(s) 1852
EarI CTCTTC 6 cut(s) 147, 271, 303, 387, 423, 2036
EciI GGCGGA 2 cut(s) 1105, 1475
Ecl136II GAGCTC 2 cut(s) 619, 1947
Eco130I CCWWGG 2 cut(s) 60, 843
Eco147I AGGCCT 1 cut(s) 1795
Eco24I GRGCYC 3 cut(s) 621, 1949, 2000
Eco31I GGTCTC 1 cut(s) 1559
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 5 cut(s) 54, 185, 1675, 1776, 2329
Eco53kI GAGCTC 2 cut(s) 619, 1947
Eco57I CTGAAG 2 cut(s) 1577, 2231
Eco72I CACGTG 1 cut(s) 2223
Eco81I CCTNAGG 3 cut(s) 24, 42, 1068
Eco88I CYCGRG 2 cut(s) 1058, 2332
EcoICRI GAGCTC 2 cut(s) 619, 1947
EcoO109I RGGNCCY 1 cut(s) 54
EcoRI GAATTC 2 cut(s) 863, 1627
EcoRII CCWGG 3 cut(s) 1157, 1547, 1905
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 2 cut(s) 60, 843
EcoT22I ATGCAT 2 cut(s) 263, 1171
EcoT38I GRGCYC 3 cut(s) 621, 1949, 2000
ErhI CCWWGG 2 cut(s) 60, 843
Esp3I CGTCTC 3 cut(s) 1727, 2081, 2375
FaeI CATG 7 cut(s) 847, 1169, 1264, 1447, 1489, 1960, 2023
FaqI GGGAC 3 cut(s) 67, 138, 171
FatI CATG 7 cut(s) 843, 1165, 1260, 1443, 1485, 1956, 2019
FauI CCCGC 2 cut(s) 1661, 1872
FbaI TGATCA 4 cut(s) 139, 810, 901, 925
FblI GTMKAC 2 cut(s) 1146, 2048
Fnu4HI GCNGC 6 cut(s) 519, 921, 973, 1458, 1599, 2243
FokI GGATG 7 cut(s) 17, 207, 410, 497, 518, 542, 575
FriOI GRGCYC 3 cut(s) 621, 1949, 2000
Fsp4HI GCNGC 6 cut(s) 519, 921, 973, 1458, 1599, 2243
FspBI CTAG 2 cut(s) 878, 1199
GlaI GCGC 4 cut(s) 1316, 1546, 1878, 1973
GluI GCNGC 6 cut(s) 519, 921, 973, 1458, 1599, 2243
GsaI CCCAGC 1 cut(s) 1592
GsuI CTGGAG 4 cut(s) 1141, 1643, 1760, 1928
HaeII RGCGCY 2 cut(s) 1318, 1975
HaeIII GGCC 5 cut(s) 740, 848, 1197, 1640, 1795
HgaI GACGC 5 cut(s) 1372, 1486, 1744, 1826, 2221
HhaI GCGC 4 cut(s) 1317, 1547, 1879, 1974
Hin1I GRCGYC 1 cut(s) 1364
Hin1II CATG 7 cut(s) 847, 1169, 1264, 1447, 1489, 1960, 2023
Hin6I GCGC 4 cut(s) 1315, 1545, 1877, 1972
HinP1I GCGC 4 cut(s) 1315, 1545, 1877, 1972
HincII GTYRAC 2 cut(s) 1686, 2049
HindII GTYRAC 2 cut(s) 1686, 2049
HindIII AAGCTT 2 cut(s) 236, 626
HphI GGTGA 7 cut(s) 362, 428, 449, 470, 557, 1322, 1894
Hpy166II GTNNAC 5 cut(s) 1147, 1678, 1686, 1695, 2049
Hpy8I GTNNAC 5 cut(s) 1147, 1678, 1686, 1695, 2049
HpyCH4III ACNGT 7 cut(s) 164, 694, 803, 959, 1470, 1576, 1802
HpyCH4IV ACGT 7 cut(s) 1221, 1829, 1935, 2090, 2222, 2324, 2384
HpyF3I CTNAG 9 cut(s) 24, 42, 821, 1068, 1394, 1709, 2057, 2129, 2258
HpySE526I ACGT 7 cut(s) 1221, 1829, 1935, 2090, 2222, 2324, 2384
Hsp92I GRCGYC 1 cut(s) 1364
Hsp92II CATG 7 cut(s) 847, 1169, 1264, 1447, 1489, 1960, 2023
HspAI GCGC 4 cut(s) 1315, 1545, 1877, 1972
KflI GGGWCCC 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 1339, 2002
KroI GCCGGC 2 cut(s) 1118, 2097
KroNI GCCGGC 2 cut(s) 1120, 2099
Ksp22I TGATCA 4 cut(s) 139, 810, 901, 925
Lsp1109I GCAGC 4 cut(s) 505, 932, 984, 2254
LweI GCATC 8 cut(s) 248, 270, 436, 544, 668, 1221, 1221, 1497
MaeI CTAG 2 cut(s) 878, 1199
MaeII ACGT 7 cut(s) 1221, 1829, 1935, 2090, 2222, 2324, 2384
MaeIII GTNAC 5 cut(s) 70, 545, 959, 980, 2234
MbiI CCGCTC 2 cut(s) 1457, 1774
MflI RGATCY 4 cut(s) 76, 214, 601, 730
MhlI GDGCHC 5 cut(s) 621, 1761, 1949, 2000, 2222
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 7 cut(s) 539, 785, 863, 1052, 1295, 1423, 1627
MluI ACGCGT 1 cut(s) 1716
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 8 cut(s) 31, 761, 905, 1031, 1337, 1775, 1862, 1892
MmeI TCCRAC 7 cut(s) 1107, 1227, 1631, 1803, 1875, 2232, 2307
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 2 cut(s) 263, 1171
MroI TCCGGA 2 cut(s) 1339, 2002
MroNI GCCGGC 2 cut(s) 1118, 2097
MroXI GAANNNNTTC 1 cut(s) 1469
MscI TGGCCA 1 cut(s) 740
MseI TTAA 4 cut(s) 32, 624, 1607, 2358
MslI CAYNNNNRTG 3 cut(s) 1106, 1574, 2225
Msp20I TGGCCA 1 cut(s) 740
MspA1I CMGCKG 3 cut(s) 183, 923, 1321
MspCI CTTAAG 1 cut(s) 623
Mva1269I GAATGC 4 cut(s) 139, 828, 1169, 1277
MvaI CCWGG 3 cut(s) 1159, 1549, 1907
MvnI CGCG 5 cut(s) 1718, 1806, 1820, 1865, 1879
NaeI GCCGGC 2 cut(s) 1120, 2099
NciI CCSGG 8 cut(s) 1059, 1060, 1536, 1658, 1665, 1690, 2333, 2334
NcoI CCATGG 1 cut(s) 843
NgoMIV GCCGGC 2 cut(s) 1118, 2097
NlaIII CATG 7 cut(s) 847, 1169, 1264, 1447, 1489, 1960, 2023
NmeAIII GCCGAG 1 cut(s) 1173
NmuCI GTSAC 3 cut(s) 70, 545, 2234
NruI TCGCGA 1 cut(s) 1806
NsiI ATGCAT 2 cut(s) 263, 1171
NspI RCATGY 2 cut(s) 1169, 1489
NspV TTCGAA 1 cut(s) 84
OliI CACNNNNGTG 2 cut(s) 1574, 2225
PaeI GCATGC 1 cut(s) 1169
PagI TCATGA 1 cut(s) 1956
PaqCI CACCTGC 1 cut(s) 672
PceI AGGCCT 1 cut(s) 1795
PciI ACATGT 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 8 cut(s) 612, 1488, 1568, 1776, 1806, 1821, 1842, 2219
PctI GAATGC 4 cut(s) 139, 828, 1169, 1277
PdiI GCCGGC 2 cut(s) 1120, 2099
PdmI GAANNNNTTC 1 cut(s) 1469
PfoI TCCNGGA 1 cut(s) 1157
PinAI ACCGGT 2 cut(s) 167, 2295
PkrI GCNGC 6 cut(s) 520, 922, 974, 1459, 1600, 2244
PleI GAGTC 8 cut(s) 31, 760, 904, 1030, 1336, 1775, 1861, 1892
PmaCI CACGTG 1 cut(s) 2223
PmlI CACGTG 1 cut(s) 2223
PpsI GAGTC 8 cut(s) 31, 760, 904, 1030, 1336, 1775, 1861, 1892
Ppu21I YACGTR 1 cut(s) 2223
PpuMI RGGWCCY 1 cut(s) 54
PscI ACATGT 1 cut(s) 1485
PshBI ATTAAT 1 cut(s) 1607
Psp124BI GAGCTC 2 cut(s) 621, 1949
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 3 cut(s) 1157, 1547, 1905
PspCI CACGTG 1 cut(s) 2223
PspFI CCCAGC 1 cut(s) 1588
PspGI CCWGG 3 cut(s) 1157, 1547, 1905
PspPI GGNCC 5 cut(s) 54, 185, 1675, 1776, 2329
PspPPI RGGWCCY 1 cut(s) 54
PsrI GAACNNNNNNTAC 2 cut(s) 2285, 2317
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 2 cut(s) 923, 1420
PsuI RGATCY 4 cut(s) 76, 214, 601, 730
PvuII CAGCTG 2 cut(s) 923, 1321
RruI TCGCGA 1 cut(s) 1806
RseI CAYNNNNRTG 3 cut(s) 1106, 1574, 2225
Rsr2I CGGWCCG 1 cut(s) 1776
RsrII CGGWCCG 1 cut(s) 1776
SacI GAGCTC 2 cut(s) 621, 1949
SalI GTCGAC 1 cut(s) 2047
SaqAI TTAA 4 cut(s) 32, 624, 1607, 2358
SatI GCNGC 6 cut(s) 519, 921, 973, 1458, 1599, 2243
Sau96I GGNCC 5 cut(s) 54, 185, 1675, 1776, 2329
SchI GAGTC 8 cut(s) 31, 761, 905, 1031, 1337, 1775, 1862, 1892
SduI GDGCHC 5 cut(s) 621, 1761, 1949, 2000, 2222
SfaNI GCATC 8 cut(s) 248, 270, 436, 544, 668, 1221, 1221, 1497
SfcI CTRYAG 2 cut(s) 519, 915
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 5 cut(s) 54, 185, 1675, 1776, 2329
SmaI CCCGGG 2 cut(s) 1060, 2334
SmiMI CAYNNNNRTG 3 cut(s) 1106, 1574, 2225
SmlI CTYRAG 3 cut(s) 191, 623, 699
SmoI CTYRAG 3 cut(s) 191, 623, 699
SphI GCATGC 1 cut(s) 1169
Sse9I AATT 7 cut(s) 539, 785, 863, 1052, 1295, 1423, 1627
SseBI AGGCCT 1 cut(s) 1795
SspMI CTAG 2 cut(s) 878, 1199
SstI GAGCTC 2 cut(s) 621, 1949
StuI AGGCCT 1 cut(s) 1795
StyI CCWWGG 2 cut(s) 60, 843
TaaI ACNGT 7 cut(s) 164, 694, 803, 959, 1470, 1576, 1802
TaiI ACGT 7 cut(s) 1224, 1832, 1938, 2093, 2225, 2327, 2387
TaqII GACCGA 1 cut(s) 754
TasI AATT 7 cut(s) 539, 785, 863, 1052, 1295, 1423, 1627
TatI WGTACW 3 cut(s) 1725, 1926, 2352
TauI GCSGC 2 cut(s) 1460, 1601
Tru1I TTAA 4 cut(s) 32, 624, 1607, 2358
Tru9I TTAA 4 cut(s) 32, 624, 1607, 2358
TscAI CASTG 3 cut(s) 982, 1241, 2239
TseFI GTSAC 3 cut(s) 70, 545, 2234
TseI GCWGC 4 cut(s) 518, 920, 972, 2242
Tsp45I GTSAC 3 cut(s) 70, 545, 2234
TspDTI ATGAA 8 cut(s) 258, 309, 525, 1191, 1715, 1945, 1976, 2195
TspGWI ACGGA 3 cut(s) 89, 1577, 2066
TspMI CCCGGG 2 cut(s) 1058, 2332
TspRI CASTG 3 cut(s) 982, 1241, 2239
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 5 cut(s) 54, 185, 1675, 1776, 2329
VspI ATTAAT 1 cut(s) 1607
XapI RAATTY 5 cut(s) 785, 863, 1052, 1423, 1627
XbaI TCTAGA 1 cut(s) 877
XceI RCATGY 2 cut(s) 1169, 1489
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 2275
XmaI CCCGGG 2 cut(s) 1058, 2332
XmiI GTMKAC 2 cut(s) 1146, 2048
XmnI GAANNNNTTC 1 cut(s) 1469
XspI CTAG 2 cut(s) 878, 1199
Zsp2I ATGCAT 2 cut(s) 263, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.