MD10G1124000.v1.1

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007697
Physical Location & Seq
Reverse (-)
20153050 .. 20153517
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1124000.v1.1.491

Sequence Viewer

Length: 468 bp
ATGATACAGCTTCTCAATTTCACGGAGGGGATTGCGATATCGAAGCGGTCGGCGGAGAAGCTGTTTAAGACGCTGGACATGTACGAGGCGTTGCGGGAGGTGATCCCCAAAATGGACAACTTGTTTCCGGCAGACTGCGTGAACGAGCTGAAGCACGAGACCTCTAATGTCCGGACCTGCCTCGGCGTGGCAGCGATATGCATTTTCTGTGATCTGGAGAACTCAATTAAGGCCGAGACGGTTAGAAAACCGGTTCCTGGAGGGGCGGTCCATCCGCTGACCCGATACACGATGAATTACCTGAGGTACGCGTGCGAGTACAAGGACACGCTCGAATTGGTTTTTTCAGAGAGCACTCGAAGATCGAACGCGCCGATTCGACGAGTCGGCCCGAACGCGACGAATACGAGGCATGGGAGGGATCCGGGAACGCGAGCGACGACCAATCGCCGTTTTACTTGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

156

Amino Acids

17.53

Weight (kDa)

9.13

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 1 - 131 5.7e-37 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
AccII CGCG 4 cut(s) 311, 371, 398, 433
AccIII TCCGGA 1 cut(s) 171
AciI CCGC 5 cut(s) 46, 53, 94, 266, 275
AclWI GGATC 3 cut(s) 97, 416, 429
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 3 cut(s) 83, 308, 320
AfiI CCNNNNNNNGG 3 cut(s) 112, 187, 257
AflIII ACRYGT 2 cut(s) 78, 309
AgeI ACCGGT 1 cut(s) 250
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 4 cut(s) 10, 61, 148, 465
AluI AGCT 4 cut(s) 10, 61, 148, 465
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 2 cut(s) 152, 230
AlwI GGATC 3 cut(s) 97, 416, 429
Aor13HI TCCGGA 1 cut(s) 171
AoxI GGCC 2 cut(s) 231, 388
ApeKI GCWGC 2 cut(s) 191, 462
AsiGI ACCGGT 1 cut(s) 250
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 3 cut(s) 174, 268, 389
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 2 cut(s) 174, 268
AxyI CCTNAGG 1 cut(s) 302
BamHI GGATCC 1 cut(s) 421
BauI CACGAG 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 356
BbvI GCAGC 1 cut(s) 203
BccI CCATC 1 cut(s) 279
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 258
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 2 cut(s) 152, 230
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 2 cut(s) 192, 463
BlsI GCNGC 2 cut(s) 193, 464
Bme1390I CCNGG 2 cut(s) 258, 426
Bme18I GGWCC 2 cut(s) 174, 268
BmgT120I GGNCC 3 cut(s) 174, 268, 389
BmiI GGNNCC 2 cut(s) 255, 423
BmrFI CCNGG 2 cut(s) 258, 426
BpmI CTGGAG 2 cut(s) 236, 279
BpuMI CCSGG 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 2 cut(s) 171, 250
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 252, 282
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 187, 257
Bse118I RCCGGY 1 cut(s) 250
Bse21I CCTNAGG 1 cut(s) 302
BseAI TCCGGA 1 cut(s) 171
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 3 cut(s) 112, 187, 257
BseMII CTCAG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 203
Bsh1236I CGCG 4 cut(s) 311, 371, 398, 433
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 233, 390
BshTI ACCGGT 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 356
BsiSI CCGG 4 cut(s) 128, 172, 251, 425
BslI CCNNNNNNNGG 3 cut(s) 112, 187, 257
BsmAI GTCTC 2 cut(s) 152, 230
BsmBI CGTCTC 1 cut(s) 230
BsnI GGCC 2 cut(s) 233, 390
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 356
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 4 cut(s) 102, 211, 362, 421
BspACI CCGC 5 cut(s) 46, 53, 94, 266, 275
BspANI GGCC 2 cut(s) 233, 390
BspCNI CTCAG 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 171
BspFNI CGCG 4 cut(s) 311, 371, 398, 433
BspLI GGNNCC 2 cut(s) 255, 423
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 3 cut(s) 97, 416, 429
BspTNI GGTCTC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 4 cut(s) 102, 211, 362, 421
BssSI CACGAG 1 cut(s) 155
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 313, 435
BstDEI CTNAG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 4 cut(s) 311, 371, 398, 433
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 4 cut(s) 105, 214, 365, 424
BstMAI GTCTC 2 cut(s) 152, 230
BstMBI GATC 4 cut(s) 102, 211, 362, 421
BstMCI CGRYCG 1 cut(s) 50
BstNI CCWGG 1 cut(s) 258
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 256, 424
BstUI CGCG 4 cut(s) 311, 371, 398, 433
BstV1I GCAGC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
Bsu36I CCTNAGG 1 cut(s) 302
BsuRI GGCC 2 cut(s) 233, 390
BtsCI GGATG 1 cut(s) 271
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 313, 435
CfoI GCGC 1 cut(s) 373
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 174, 268, 389
CseI GACGC 1 cut(s) 79
Csp6I GTAC 3 cut(s) 82, 307, 319
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 2 cut(s) 79, 413
CviJI RGCY 6 cut(s) 10, 61, 148, 233, 390, 465
CviKI_1 RGCY 6 cut(s) 10, 61, 148, 233, 390, 465
CviQI GTAC 3 cut(s) 82, 307, 319
DdeI CTNAG 1 cut(s) 302
DpnI GATC 4 cut(s) 104, 213, 364, 423
DpnII GATC 4 cut(s) 102, 211, 362, 421
EciI GGCGGA 1 cut(s) 68
Eco31I GGTCTC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 174, 268
Eco57I CTGAAG 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 256
EcoRV GATATC 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 2 cut(s) 82, 416
FaiI YATR 3 cut(s) 80, 199, 414
FatI CATG 2 cut(s) 78, 412
FauI CCCGC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 192, 463
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 2 cut(s) 192, 463
GlaI GCGC 1 cut(s) 372
GluI GCNGC 2 cut(s) 192, 463
GsuI CTGGAG 2 cut(s) 236, 279
HaeIII GGCC 2 cut(s) 233, 390
HapII CCGG 4 cut(s) 128, 172, 251, 425
HgaI GACGC 1 cut(s) 79
HhaI GCGC 1 cut(s) 373
Hin1II CATG 2 cut(s) 82, 416
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HinfI GANTC 2 cut(s) 376, 384
HpaII CCGG 4 cut(s) 128, 172, 251, 425
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 2 cut(s) 172, 215
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 3 cut(s) 384, 403, 442
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 2 cut(s) 201, 462
HpyF3I CTNAG 1 cut(s) 302
Hsp92II CATG 2 cut(s) 82, 416
HspAI GCGC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 171
Kzo9I GATC 4 cut(s) 102, 211, 362, 421
Lsp1109I GCAGC 1 cut(s) 203
MalI GATC 4 cut(s) 104, 213, 364, 423
MboI GATC 4 cut(s) 102, 211, 362, 421
MboII GAAGA 1 cut(s) 372
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 4 cut(s) 16, 225, 295, 335
MluI ACGCGT 1 cut(s) 309
MlyI GAGTC 1 cut(s) 393
MnlI CCTC 9 cut(s) 19, 79, 91, 172, 191, 254, 297, 402, 411
Mph1103I ATGCAT 1 cut(s) 203
MroI TCCGGA 1 cut(s) 171
MseI TTAA 2 cut(s) 66, 228
MspA1I CMGCKG 2 cut(s) 277, 465
MspI CCGG 4 cut(s) 128, 172, 251, 425
MspR9I CCNGG 2 cut(s) 258, 426
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 4 cut(s) 311, 371, 398, 433
NciI CCSGG 1 cut(s) 426
NdeII GATC 4 cut(s) 102, 211, 362, 421
NlaIII CATG 2 cut(s) 82, 416
NlaIV GGNNCC 2 cut(s) 255, 423
NmeAIII GCCGAG 2 cut(s) 162, 259
NsiI ATGCAT 1 cut(s) 203
NspI RCATGY 1 cut(s) 82
PciI ACATGT 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 371, 437
PfeI GAWTC 1 cut(s) 376
PfoI TCCNGGA 2 cut(s) 256, 424
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 2 cut(s) 193, 464
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
PscI ACATGT 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 2 cut(s) 255, 423
PspPI GGNCC 3 cut(s) 174, 268, 389
PsuI RGATCY 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 465
RsaI GTAC 3 cut(s) 83, 308, 320
RsaNI GTAC 3 cut(s) 82, 307, 319
SaqAI TTAA 2 cut(s) 66, 228
SatI GCNGC 2 cut(s) 192, 463
Sau3AI GATC 4 cut(s) 102, 211, 362, 421
Sau96I GGNCC 3 cut(s) 174, 268, 389
SchI GAGTC 1 cut(s) 393
ScrFI CCNGG 2 cut(s) 258, 426
SduI GDGCHC 1 cut(s) 356
SetI ASST 9 cut(s) 12, 63, 102, 150, 164, 179, 303, 308, 467
SinI GGWCC 2 cut(s) 174, 268
Sse9I AATT 4 cut(s) 16, 225, 295, 335
SsiI CCGC 5 cut(s) 46, 53, 94, 266, 275
StyD4I CCNGG 2 cut(s) 256, 424
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 5 cut(s) 41, 333, 358, 365, 379
TasI AATT 4 cut(s) 16, 225, 295, 335
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 66, 228
Tru9I TTAA 2 cut(s) 66, 228
TseI GCWGC 2 cut(s) 191, 462
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 38
VpaK11BI GGWCC 2 cut(s) 174, 268
XceI RCATGY 1 cut(s) 82
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.