MD01G1065300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007895
Physical Location & Seq
Reverse (-)
16894983 .. 16896733
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1065300.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGCAAACCCAACACCTCCAGTCCCCTTTCTCCTCGCCCTTCTCCTCCTCCTTATTCTGCCCTTCATCTTTTCCCCAAGTGGCGAATTCGTCCGGTTCGCCGAGGCGGGAAAGCGCAGAGTCCACATCACCGACGACCTGGACGACGTCCTTGACGACGAGGAGGACGACACCTGGAAAGAATGGGGCAAGAAAACGAAACCCTCTTCCGATTTTGATCCGCCGCCCGATTTGTCGAAGATGGACATGTCGCAGATACAGGCGGAGATGATGAAACGGCAAACCGGGCCGGCTTTCGGGTTCGTGAAGCTGCGGATGGGCGAGAAGCGGACTCGGGATACAGTGGCCGAGCTTGCTATGAAGTGGACTCAAGTTCTGAGGACCGGAGCAATTGAGGCAAGGTTCATGGGTGTTGATCTAAACACGATCATGTTCAACATGGAAAGAGGCCAAGACACAACAGAGTTGAAGGAGTTTGCGTTGAAACAACCAGAGGCCTATGAGATAAAGATTGGCGATCAAGTTTTTCGAAGACCAGGAGATCCTCCTTTAGAGGAACTCGTTGAGAAGCTCCGGGATGAGAAAAAGAGAAAGGAGAGTGACAGTGCAACAGAGAGCAATATACATTTGAAAGAGGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mesd

Protein Analysis

215

Amino Acids

24.57

Weight (kDa)

4.97

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mesd PF10185 46 - 177 9e-07 Chaperone for wingless signalling and trafficking of LDL receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AciI CCGC 6 cut(s) 107, 221, 224, 263, 313, 328
AclWI GGATC 2 cut(s) 212, 536
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 4 cut(s) 436, 469, 484, 631
AjnI CCWGG 3 cut(s) 138, 173, 535
AluBI AGCT 3 cut(s) 310, 352, 571
AluI AGCT 3 cut(s) 310, 352, 571
AlwI GGATC 2 cut(s) 212, 536
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 4 cut(s) 287, 345, 448, 495
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 287, 381
AsuC2I CCSGG 2 cut(s) 286, 575
AsuHPI GGTGA 1 cut(s) 121
AsuII TTCGAA 1 cut(s) 529
AvaI CYCGRG 1 cut(s) 333
AvaII GGWCC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 297
BccI CCATC 2 cut(s) 235, 310
BceAI ACGGC 1 cut(s) 293
BciT130I CCWGG 3 cut(s) 140, 175, 537
BciVI GTATCC 1 cut(s) 331
BcnI CCSGG 2 cut(s) 286, 575
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 2 cut(s) 224, 311
BlsI GCNGC 2 cut(s) 225, 312
Bme1390I CCNGG 5 cut(s) 140, 175, 286, 537, 575
Bme18I GGWCC 1 cut(s) 381
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 2 cut(s) 287, 381
BmrFI CCNGG 5 cut(s) 140, 175, 286, 537, 575
BpiI GAAGAC 1 cut(s) 538
Bpu14I TTCGAA 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 354
BpuMI CCSGG 2 cut(s) 286, 575
BsaBI GATNNNNATC 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 2 cut(s) 93, 383
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 20
Bse8I GATNNNNATC 1 cut(s) 216
BseBI CCWGG 3 cut(s) 140, 175, 537
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 2 cut(s) 321, 583
BseJI GATNNNNATC 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 4 cut(s) 23, 35, 38, 176
BseXI GCAGC 1 cut(s) 297
BshFI GGCC 4 cut(s) 289, 347, 450, 497
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 5 cut(s) 94, 285, 290, 384, 574
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 4 cut(s) 289, 347, 450, 497
BsoBI CYCGRG 1 cut(s) 333
Bsp119I TTCGAA 1 cut(s) 529
Bsp143I GATC 5 cut(s) 217, 415, 426, 517, 541
BspACI CCGC 6 cut(s) 107, 221, 224, 263, 313, 328
BspANI GGCC 4 cut(s) 289, 347, 450, 497
BspCNI CTCAG 1 cut(s) 369
BspPI GGATC 2 cut(s) 212, 536
BspT104I TTCGAA 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 5 cut(s) 217, 415, 426, 517, 541
BssNI GRCGYC 1 cut(s) 147
Bst2UI CCWGG 3 cut(s) 140, 175, 537
Bst4CI ACNGT 2 cut(s) 343, 605
Bst6I CTCTTC 1 cut(s) 211
BstACI GRCGYC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 529
BstC8I GCNNGC 2 cut(s) 291, 354
BstDEI CTNAG 1 cut(s) 377
BstF5I GGATG 2 cut(s) 321, 583
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 5 cut(s) 220, 418, 429, 520, 544
BstMBI GATC 5 cut(s) 217, 415, 426, 517, 541
BstMWI GCNNNNNNNGC 3 cut(s) 286, 353, 395
BstNI CCWGG 3 cut(s) 140, 175, 537
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 5 cut(s) 138, 173, 284, 535, 573
BstV1I GCAGC 1 cut(s) 297
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 4 cut(s) 289, 347, 450, 497
BtsCI GGATG 2 cut(s) 321, 583
BtsIMutI CAGTG 2 cut(s) 348, 610
Cac8I GCNNGC 2 cut(s) 291, 354
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 287, 381
CviAII CATG 4 cut(s) 247, 406, 430, 439
CviJI RGCY 8 cut(s) 289, 293, 310, 347, 352, 450, 497, 571
CviKI_1 RGCY 8 cut(s) 289, 293, 310, 347, 352, 450, 497, 571
DdeI CTNAG 1 cut(s) 377
DpnI GATC 5 cut(s) 219, 417, 428, 519, 543
DpnII GATC 5 cut(s) 217, 415, 426, 517, 541
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
EciI GGCGGA 2 cut(s) 210, 278
Eco147I AGGCCT 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 3 cut(s) 138, 173, 535
FaeI CATG 4 cut(s) 250, 409, 433, 442
FaiI YATR 7 cut(s) 248, 359, 407, 431, 440, 501, 623
FaqI GGGAC 1 cut(s) 8
FatI CATG 4 cut(s) 246, 405, 429, 438
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 224, 311
FokI GGATG 2 cut(s) 328, 590
Fsp4HI GCNGC 2 cut(s) 224, 311
GlaI GCGC 1 cut(s) 116
GluI GCNGC 2 cut(s) 224, 311
HaeIII GGCC 4 cut(s) 289, 347, 450, 497
HapII CCGG 5 cut(s) 94, 285, 290, 384, 574
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 250, 409, 433, 442
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 3 cut(s) 120, 331, 367
HpaII CCGG 5 cut(s) 94, 285, 290, 384, 574
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 124, 366
Hpy188I TCNGA 2 cut(s) 211, 378
Hpy188III TCNNGA 2 cut(s) 304, 335
Hpy8I GTNNAC 2 cut(s) 124, 366
Hpy99I CGWCG 3 cut(s) 137, 149, 161
HpyAV CCTTC 3 cut(s) 50, 73, 463
HpyCH4III ACNGT 2 cut(s) 343, 605
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 1 cut(s) 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 286, 353, 395
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 250, 409, 433, 442
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 289
KroNI GCCGGC 1 cut(s) 291
Kzo9I GATC 5 cut(s) 217, 415, 426, 517, 541
LmnI GCTCC 2 cut(s) 386, 576
Lsp1109I GCAGC 1 cut(s) 297
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 599
MalI GATC 5 cut(s) 219, 417, 428, 519, 543
MboI GATC 5 cut(s) 217, 415, 426, 517, 541
MboII GAAGA 3 cut(s) 198, 250, 543
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 1 cut(s) 541
MluCI AATT 3 cut(s) 86, 390, 638
MlyI GAGTC 3 cut(s) 129, 325, 361
MroNI GCCGGC 1 cut(s) 289
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 5 cut(s) 94, 285, 290, 384, 574
MspR9I CCNGG 5 cut(s) 140, 175, 286, 537, 575
MunI CAATTG 1 cut(s) 390
MvaI CCWGG 3 cut(s) 140, 175, 537
MwoI GCNNNNNNNGC 3 cut(s) 286, 353, 395
NaeI GCCGGC 1 cut(s) 291
NciI CCSGG 2 cut(s) 286, 575
NdeII GATC 5 cut(s) 217, 415, 426, 517, 541
NgoMIV GCCGGC 1 cut(s) 289
NlaIII CATG 4 cut(s) 250, 409, 433, 442
NmeAIII GCCGAG 2 cut(s) 127, 373
NmuCI GTSAC 1 cut(s) 599
NspI RCATGY 1 cut(s) 250
NspV TTCGAA 1 cut(s) 529
PceI AGGCCT 1 cut(s) 497
PciI ACATGT 1 cut(s) 246
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 153, 165
PdiI GCCGGC 1 cut(s) 291
PflFI GACNNNGTC 1 cut(s) 146
PfoI TCCNGGA 1 cut(s) 573
PkrI GCNGC 2 cut(s) 225, 312
PleI GAGTC 3 cut(s) 128, 325, 361
PpsI GAGTC 3 cut(s) 128, 325, 361
PscI ACATGT 1 cut(s) 246
Psp6I CCWGG 3 cut(s) 138, 173, 535
PspGI CCWGG 3 cut(s) 138, 173, 535
PspPI GGNCC 2 cut(s) 287, 381
PsuI RGATCY 1 cut(s) 541
PsyI GACNNNGTC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 428
SatI GCNGC 2 cut(s) 224, 311
Sau3AI GATC 5 cut(s) 217, 415, 426, 517, 541
Sau96I GGNCC 2 cut(s) 287, 381
SchI GAGTC 3 cut(s) 129, 325, 361
ScrFI CCNGG 5 cut(s) 140, 175, 286, 537, 575
SetI ASST 8 cut(s) 19, 141, 150, 176, 312, 354, 404, 573
SfuI TTCGAA 1 cut(s) 529
SinI GGWCC 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
Sse9I AATT 3 cut(s) 86, 390, 638
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 6 cut(s) 107, 221, 224, 263, 313, 328
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 5 cut(s) 138, 173, 284, 535, 573
TaaI ACNGT 2 cut(s) 343, 605
TaiI ACGT 1 cut(s) 150
TaqI TCGA 2 cut(s) 236, 529
TasI AATT 3 cut(s) 86, 390, 638
TauI GCSGC 1 cut(s) 226
TscAI CASTG 2 cut(s) 348, 610
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 4 cut(s) 55, 287, 374, 394
TspRI CASTG 2 cut(s) 348, 610
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 381
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 250
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.