Rorug01G0249700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007895
Physical Location & Seq
Forward (+)
35791302 .. 35794973
3672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0249700.1

Sequence Viewer

Length: 720 bp
ATGGTGTATTTGATATCATCAATCTGCAATCTAGGGTTTCTTGACACACTTAGCGAAACCTCCTTCCCATCCTCTGCACCGCCACTGAAACCCTCCTCAACGACGCCGGAGACCTCTCGGTGGGCCCGCAACTCGTATCTCTCTCTCTCTCTGTGGACTGTGTACAGGTCATTGAAGGATCTTTTGGGTGATGGGGAAGATTGTGTGGGGACGTTTGTGCAAGTTACGGTTTTGGAGATTTATAATGAGGAGATTTATGATGTGTTGTCTAGTTATGGTGGGTTTCGATGGCCTAGAGGGAATGGCTCCAATTCTAAGATCACTGATTTGCCATTTTCTCTCTACTCCACATTTGTGGTTGAGGCACGTCATAGTTTCAATAAGCAAATGATATGGTTATTCTTCAGAGATATGTTGAAAGGAATTTGCCTTATGGCTATTCTTGGTCCACCAATTGTGTCTGCAATTATTGTGATTGTGCAGCACACCATAATACCTGTCCAGCACCTAGTTAGCTTTGCTCTTAACTGGCTGATGCTTTTGCTACGGAGCTTGGTTATGCCGCTTCACTTCGAGCTGGTCTTGTTAGGTTACAGAGAAACTGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTCATTCCTGATGATCCAAAATCTGCAGAAAGTATAAAACAATTGGAGGAGGTCCTTCCAGGTTTTATTGATGCCAAAGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mesd

Protein Analysis

239

Amino Acids

26.74

Weight (kDa)

5.22

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48_N PF16491 105 - 188 1.2e-19 CAAX prenyl protease N-terminal, five membrane helices
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 243
AciI CCGC 3 cut(s) 80, 127, 563
AclWI GGATC 2 cut(s) 186, 641
AcsI RAATTY 1 cut(s) 423
AcuI CTGAAG 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 3 cut(s) 175, 379, 418
AjiI CACGTC 1 cut(s) 368
AjnI CCWGG 1 cut(s) 691
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 4 cut(s) 516, 552, 577, 717
AluI AGCT 4 cut(s) 516, 552, 577, 717
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 2 cut(s) 186, 641
AoxI GGCC 3 cut(s) 123, 290, 606
ApaI GGGCCC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 481, 714
ApoI RAATTY 1 cut(s) 423
AspS9I GGNCC 5 cut(s) 123, 124, 446, 606, 685
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 2 cut(s) 446, 685
BaeGI GKGCMC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 493
BccI CCATC 3 cut(s) 76, 185, 282
BciT130I CCWGG 1 cut(s) 693
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 4 cut(s) 32, 270, 294, 509
BfmI CTRYAG 1 cut(s) 657
BisI GCNGC 3 cut(s) 482, 563, 715
BlsI GCNGC 3 cut(s) 483, 564, 716
Bme1390I CCNGG 1 cut(s) 693
Bme18I GGWCC 2 cut(s) 446, 685
BmgBI CACGTC 1 cut(s) 368
BmgT120I GGNCC 5 cut(s) 123, 124, 446, 606, 685
BmiI GGNNCC 3 cut(s) 125, 307, 607
BmrFI CCNGG 1 cut(s) 693
BmrI ACTGGG 1 cut(s) 612
BmsI GCATC 2 cut(s) 525, 694
BmuI ACTGGG 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 533, 607
Bse3DI GCAATG 1 cut(s) 624
BseBI CCWGG 1 cut(s) 693
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 624
BseNI ACTGG 2 cut(s) 533, 607
BseRI GAGGAG 3 cut(s) 85, 263, 695
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 1 cut(s) 493
BsgI GTGCAG 2 cut(s) 60, 500
BshFI GGCC 3 cut(s) 125, 292, 608
BsiSI CCGG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 3 cut(s) 125, 292, 608
Bso31I GGTCTC 1 cut(s) 104
Bsp120I GGGCCC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 127
Bsp1407I TGTACA 1 cut(s) 162
Bsp143I GATC 4 cut(s) 178, 318, 631, 646
BspACI CCGC 3 cut(s) 80, 127, 563
BspANI GGCC 3 cut(s) 125, 292, 608
BspLI GGNNCC 3 cut(s) 125, 307, 607
BspMAI CTGCAG 1 cut(s) 661
BspPI GGATC 2 cut(s) 186, 641
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 624
BsrGI TGTACA 1 cut(s) 162
BsrI ACTGG 2 cut(s) 533, 607
BssMI GATC 4 cut(s) 178, 318, 631, 646
BssNI GRCGYC 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 2 cut(s) 160, 229
BstACI GRCGYC 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 2 cut(s) 50, 315
BstF5I GGATG 1 cut(s) 68
BstKTI GATC 4 cut(s) 181, 321, 634, 649
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 4 cut(s) 178, 318, 631, 646
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstNI CCWGG 1 cut(s) 693
BstSCI CCNGG 1 cut(s) 691
BstSFI CTRYAG 1 cut(s) 657
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 493
BstX2I RGATCY 1 cut(s) 178
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 3 cut(s) 125, 292, 608
BtrI CACGTC 1 cut(s) 368
BtsCI GGATG 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 83, 321
Cac8I GCNNGC 1 cut(s) 127
Cfr13I GGNCC 5 cut(s) 123, 124, 446, 606, 685
CseI GACGC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 163
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 2 cut(s) 50, 315
DpnI GATC 4 cut(s) 180, 320, 633, 648
DpnII GATC 4 cut(s) 178, 318, 631, 646
Eco24I GRGCYC 1 cut(s) 127
Eco31I GGTCTC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 446, 685
Eco57I CTGAAG 1 cut(s) 388
EcoO109I RGGNCCY 2 cut(s) 606, 685
EcoRII CCWGG 1 cut(s) 691
EcoRV GATATC 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 127
FaqI GGGAC 1 cut(s) 223
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 3 cut(s) 482, 563, 715
FokI GGATG 1 cut(s) 55
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 3 cut(s) 482, 563, 715
FspBI CTAG 4 cut(s) 32, 270, 294, 509
GluI GCNGC 3 cut(s) 482, 563, 715
HaeIII GGCC 3 cut(s) 125, 292, 608
HapII CCGG 1 cut(s) 107
HgaI GACGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 104
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 3 cut(s) 156, 163, 449
Hpy188I TCNGA 1 cut(s) 407
Hpy188III TCNNGA 2 cut(s) 41, 641
Hpy8I GTNNAC 3 cut(s) 156, 163, 449
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 4 cut(s) 73, 169, 616, 698
HpyCH4III ACNGT 2 cut(s) 160, 229
HpyCH4IV ACGT 2 cut(s) 212, 367
HpyCH4V TGCA 7 cut(s) 27, 77, 220, 464, 481, 617, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 2 cut(s) 50, 315
HpySE526I ACGT 2 cut(s) 212, 367
Hsp92I GRCGYC 1 cut(s) 104
Kzo9I GATC 4 cut(s) 178, 318, 631, 646
LmnI GCTCC 2 cut(s) 311, 549
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 2 cut(s) 525, 694
MaeI CTAG 4 cut(s) 32, 270, 294, 509
MaeII ACGT 2 cut(s) 212, 367
MaeIII GTNAC 2 cut(s) 223, 590
MalI GATC 4 cut(s) 180, 320, 633, 648
MboI GATC 4 cut(s) 178, 318, 631, 646
MboII GAAGA 2 cut(s) 209, 394
MfeI CAATTG 2 cut(s) 453, 674
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 5 cut(s) 310, 423, 453, 465, 674
MseI TTAA 1 cut(s) 525
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 717
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 693
MunI CAATTG 2 cut(s) 453, 674
MvaI CCWGG 1 cut(s) 693
MwoI GCNNNNNNNGC 1 cut(s) 614
NdeII GATC 4 cut(s) 178, 318, 631, 646
NlaIV GGNNCC 3 cut(s) 125, 307, 607
OliI CACNNNNGTG 1 cut(s) 353
PkrI GCNGC 3 cut(s) 483, 564, 716
PpuMI RGGWCCY 1 cut(s) 685
PsiI TTATAA 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 685
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 3 cut(s) 125, 307, 607
PspOMI GGGCCC 1 cut(s) 123
PspPI GGNCC 5 cut(s) 123, 124, 446, 606, 685
PspPPI RGGWCCY 1 cut(s) 685
PstI CTGCAG 1 cut(s) 661
PsuI RGATCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 717
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 3 cut(s) 482, 563, 715
Sau3AI GATC 4 cut(s) 178, 318, 631, 646
Sau96I GGNCC 5 cut(s) 123, 124, 446, 606, 685
ScrFI CCNGG 1 cut(s) 693
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 2 cut(s) 525, 694
SfcI CTRYAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 446, 685
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 5 cut(s) 310, 423, 453, 465, 674
SsiI CCGC 3 cut(s) 80, 127, 563
SspMI CTAG 4 cut(s) 32, 270, 294, 509
StyD4I CCNGG 1 cut(s) 691
TaaI ACNGT 2 cut(s) 160, 229
TaiI ACGT 2 cut(s) 215, 370
TaqI TCGA 2 cut(s) 286, 573
TasI AATT 5 cut(s) 310, 423, 453, 465, 674
TatI WGTACW 1 cut(s) 162
TauI GCSGC 1 cut(s) 565
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 2 cut(s) 90, 328
TseI GCWGC 2 cut(s) 481, 714
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 2 cut(s) 90, 328
VpaK11BI GGWCC 2 cut(s) 446, 685
XapI RAATTY 1 cut(s) 423
XspI CTAG 4 cut(s) 32, 270, 294, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.