MD07G1135200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007895
Physical Location & Seq
Forward (+)
19723843 .. 19727815
3973 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1135200.v1.1.491

Sequence Viewer

Length: 729 bp
ATGCAAACAATTAAAATTAAAAAAGAAAAGAAAAAAGACATAGATCCGAAGTACTGTTTCCCTCTCCAAATCTTCACACAAAGCATGGCAAACCCGACACCTCCAGTCCCCATTCTCCTCGCCCTTCTCCTTCTCCTCATTCTACCCTTCATCCTTTCCTCAAATGGCGAATTCGTCCGGTTCGCCGAGGCGGGAAAGCGGAGAGTCCACATCACCGACGACCTGGACGACGTAGTCGACGAGGAGGAGGACGACACCTGGAAAGAATGGGGCAAGAAATCGAAACCCTCTTCCGATTTCGATCCGCTTCCTGATCTGTCGAAGATGGACATGTCGCAGATCCAGGCGGAGATGATGAAGCGGCAGACCGGGCCGGCTTTCGGGTTCGTGAAGCTCCGGGTGGGCGAGAAACGGAGTCGGGATACGGTGGCTGAGCTTGCTATGAAATGGACTCAAGTTCTGAGGACCGGAGCCATTGGGGCAAGGTTCATGGGTGTTGATCTAAACACGATCATGTTCACCATGGAAAGAGGCCAAGACACGACAGAGTTGAAGGAGTTTGCATTGAACCAACCAGAGGCCTATGAGATAAAGATTGGTGATCAAGTTTTTCGAAGACTAGGAGATCCTCCTTTAGAGGAACTCGTTGAGAAGCTCCAGAATGAGAAAAAGAGAAAGGAGAGTGATAGTCCACCAGAGAGCGATACACATTTGAAAGAGGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mesd

Protein Analysis

243

Amino Acids

27.76

Weight (kDa)

5.25

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mesd PF10185 102 - 205 3.5e-06 Chaperone for wingless signalling and trafficking of LDL receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 237
AciI CCGC 5 cut(s) 191, 199, 305, 347, 361
AclWI GGATC 4 cut(s) 38, 296, 334, 620
AcsI RAATTY 1 cut(s) 170
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 2 cut(s) 380, 577
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 3 cut(s) 553, 568, 715
AjnI CCWGG 3 cut(s) 222, 257, 342
AluBI AGCT 3 cut(s) 394, 436, 655
AluI AGCT 3 cut(s) 394, 436, 655
AlwI GGATC 4 cut(s) 38, 296, 334, 620
AoxI GGCC 3 cut(s) 371, 532, 579
ApoI RAATTY 1 cut(s) 170
AspS9I GGNCC 2 cut(s) 371, 465
AsuC2I CCSGG 2 cut(s) 370, 398
AsuHPI GGTGA 3 cut(s) 205, 511, 611
AsuII TTCGAA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 622
BccI CCATC 1 cut(s) 319
BciT130I CCWGG 3 cut(s) 224, 259, 344
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 2 cut(s) 370, 398
BfaI CTAG 1 cut(s) 620
BfuI GTATCC 1 cut(s) 415
BglI GCCNNNNNGGC 1 cut(s) 479
BisI GCNGC 1 cut(s) 362
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 363
BmcAI AGTACT 1 cut(s) 53
Bme1390I CCNGG 5 cut(s) 224, 259, 344, 370, 398
Bme18I GGWCC 1 cut(s) 465
BmgT120I GGNCC 2 cut(s) 371, 465
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 5 cut(s) 224, 259, 344, 370, 398
BpiI GAAGAC 1 cut(s) 622
BpmI CTGGAG 2 cut(s) 87, 641
Bpu1102I GCTNAGC 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 613
BpuEI CTTGAG 1 cut(s) 438
BpuMI CCSGG 2 cut(s) 370, 398
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 186, 522
BsaWI WCCGGW 2 cut(s) 177, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 577
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 104
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 3 cut(s) 224, 259, 344
BseDI CCNNGG 2 cut(s) 186, 522
BseGI GGATG 1 cut(s) 150
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 2 cut(s) 380, 577
BseMII CTCAG 2 cut(s) 423, 452
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 4 cut(s) 107, 125, 257, 260
BshFI GGCC 3 cut(s) 373, 534, 581
BsiSI CCGG 5 cut(s) 178, 369, 374, 397, 468
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 380, 577
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 373, 534, 581
Bsp119I TTCGAA 1 cut(s) 613
Bsp143I GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
Bsp1720I GCTNAGC 1 cut(s) 432
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 5 cut(s) 191, 199, 305, 347, 361
BspANI GGCC 3 cut(s) 373, 534, 581
BspCNI CTCAG 2 cut(s) 424, 453
BspLI GGNNCC 1 cut(s) 472
BspPI GGATC 4 cut(s) 38, 296, 334, 620
BspT104I TTCGAA 1 cut(s) 613
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 2 cut(s) 186, 522
BssMI GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 224, 259, 344
Bst4CI ACNGT 2 cut(s) 56, 427
Bst6I CTCTTC 1 cut(s) 295
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 375, 438
BstDEI CTNAG 2 cut(s) 432, 461
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 8 cut(s) 46, 304, 316, 342, 502, 513, 604, 628
BstMBI GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
BstMWI GCNNNNNNNGC 3 cut(s) 370, 437, 479
BstNI CCWGG 3 cut(s) 224, 259, 344
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 5 cut(s) 222, 257, 342, 368, 396
BstV2I GAAGAC 1 cut(s) 622
BstX2I RGATCY 3 cut(s) 43, 339, 625
BstYI RGATCY 3 cut(s) 43, 339, 625
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 3 cut(s) 373, 534, 581
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 375, 438
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 2 cut(s) 371, 465
Csp6I GTAC 1 cut(s) 52
CviAII CATG 5 cut(s) 85, 331, 490, 514, 523
CviJI RGCY 9 cut(s) 373, 377, 394, 431, 436, 473, 534, 581, 655
CviKI_1 RGCY 9 cut(s) 373, 377, 394, 431, 436, 473, 534, 581, 655
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 2 cut(s) 432, 461
DpnI GATC 8 cut(s) 45, 303, 315, 341, 501, 512, 603, 627
DpnII GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
EciI GGCGGA 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 522
Eco147I AGGCCT 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 3 cut(s) 222, 257, 342
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 5 cut(s) 88, 334, 493, 517, 526
FaiI YATR 8 cut(s) 41, 86, 332, 443, 491, 515, 524, 585
FaqI GGGAC 1 cut(s) 92
FatI CATG 5 cut(s) 84, 330, 489, 513, 522
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 362
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 1 cut(s) 362
FspBI CTAG 1 cut(s) 620
GluI GCNGC 1 cut(s) 362
GsuI CTGGAG 2 cut(s) 87, 641
HaeIII GGCC 3 cut(s) 373, 534, 581
HapII CCGG 5 cut(s) 178, 369, 374, 397, 468
Hin1II CATG 5 cut(s) 88, 334, 493, 517, 526
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 3 cut(s) 204, 415, 451
HpaII CCGG 5 cut(s) 178, 369, 374, 397, 468
HphI GGTGA 3 cut(s) 205, 511, 611
Hpy166II GTNNAC 4 cut(s) 208, 238, 519, 692
Hpy188I TCNGA 3 cut(s) 48, 295, 462
Hpy188III TCNNGA 4 cut(s) 311, 388, 419, 658
Hpy8I GTNNAC 4 cut(s) 208, 238, 519, 692
Hpy99I CGWCG 3 cut(s) 221, 233, 242
HpyAV CCTTC 4 cut(s) 134, 140, 157, 547
HpyCH4III ACNGT 2 cut(s) 56, 427
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 2 cut(s) 4, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 370, 437, 479
HpyF3I CTNAG 2 cut(s) 432, 461
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 5 cut(s) 88, 334, 493, 517, 526
KroI GCCGGC 1 cut(s) 373
KroNI GCCGGC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
LmnI GCTCC 3 cut(s) 399, 470, 660
MaeI CTAG 1 cut(s) 620
MaeII ACGT 1 cut(s) 231
MalI GATC 8 cut(s) 45, 303, 315, 341, 501, 512, 603, 627
MboI GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
MboII GAAGA 4 cut(s) 64, 282, 334, 627
MflI RGATCY 3 cut(s) 43, 339, 625
MluCI AATT 4 cut(s) 9, 15, 170, 722
MlyI GAGTC 3 cut(s) 213, 424, 445
MroNI GCCGGC 1 cut(s) 373
MseI TTAA 2 cut(s) 12, 18
MslI CAYNNNNRTG 1 cut(s) 512
MspI CCGG 5 cut(s) 178, 369, 374, 397, 468
MspR9I CCNGG 5 cut(s) 224, 259, 344, 370, 398
MvaI CCWGG 3 cut(s) 224, 259, 344
MwoI GCNNNNNNNGC 3 cut(s) 370, 437, 479
NaeI GCCGGC 1 cut(s) 375
NciI CCSGG 2 cut(s) 370, 398
NcoI CCATGG 1 cut(s) 522
NdeII GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
NgoMIV GCCGGC 1 cut(s) 373
NlaIII CATG 5 cut(s) 88, 334, 493, 517, 526
NlaIV GGNNCC 1 cut(s) 472
NmeAIII GCCGAG 1 cut(s) 211
NspI RCATGY 1 cut(s) 334
NspV TTCGAA 1 cut(s) 613
PceI AGGCCT 1 cut(s) 581
PciI ACATGT 1 cut(s) 330
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 234, 237
PdiI GCCGGC 1 cut(s) 375
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 363
PleI GAGTC 3 cut(s) 212, 423, 445
PpsI GAGTC 3 cut(s) 212, 423, 445
PscI ACATGT 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 222, 257, 342
PspGI CCWGG 3 cut(s) 222, 257, 342
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 2 cut(s) 371, 465
PsuI RGATCY 3 cut(s) 43, 339, 625
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 512
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 2 cut(s) 12, 18
SatI GCNGC 1 cut(s) 362
Sau3AI GATC 8 cut(s) 43, 301, 313, 339, 499, 510, 601, 625
Sau96I GGNCC 2 cut(s) 371, 465
ScaI AGTACT 1 cut(s) 53
SchI GAGTC 3 cut(s) 213, 424, 445
ScrFI CCNGG 5 cut(s) 224, 259, 344, 370, 398
SetI ASST 8 cut(s) 103, 225, 234, 260, 396, 438, 488, 657
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
Sse9I AATT 4 cut(s) 9, 15, 170, 722
SseBI AGGCCT 1 cut(s) 581
SsiI CCGC 5 cut(s) 191, 199, 305, 347, 361
SspMI CTAG 1 cut(s) 620
StuI AGGCCT 1 cut(s) 581
StyD4I CCNGG 5 cut(s) 222, 257, 342, 368, 396
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 2 cut(s) 56, 427
TaiI ACGT 1 cut(s) 234
TaqI TCGA 5 cut(s) 237, 281, 300, 320, 613
TasI AATT 4 cut(s) 9, 15, 170, 722
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 12, 18
Tru9I TTAA 2 cut(s) 12, 18
TspDTI ATGAA 4 cut(s) 139, 371, 458, 478
TspGWI ACGGA 1 cut(s) 427
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 465
XapI RAATTY 1 cut(s) 170
XceI RCATGY 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 1 cut(s) 620
ZrmI AGTACT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.