pycom01g09460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007895
Physical Location & Seq
Reverse (-)
10347066 .. 10348631
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09460.1

Sequence Viewer

Length: 549 bp
ATGGCAACCCCAACACCTCCAGTCCCCCTTCTCCTCGCCCTTCTCCTCCTCCTTATTCTGCCCTTCATCTTTTCCCCAAATGGCGTCCGGTTCGCCGAGGCGGGAAAGCGGAGAGTCCACATCACCGACGACCTGGACGACGTCGTCGACGATGAGGAGGACGACACCTGGAAAGAATGGGGCAAGAAAACGAAACCCTCTTCCGATTTCGATCCGGCGCCCGATCTGTCGAAGATGGACATGTCGCAGATACAGGCGGAGATGATGAAACGGCAGACCGGGCCGGCTTTCGGGTTCGTGAAGCTCCGGATGGGCGAGAAGCGGACTCGGGATACAGTGGCCGAGCTTGCTATGAAGTGGACTCAAGTTCTGAGGACCGGAGCAATTGAGGCAAGGTTCATGGGTGTTGATCTAAACACGATCATGTTCAACATGGAAAGAGGCCAAGACACAACAGAGTTGAAGGAGTTTACGTTGAAACAACCAGAGGCCTACGAGATAAAGATTGGCGATCAAGTGTTTCGAAGACCAGGATCGATCCTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mesd

Protein Analysis

183

Amino Acids

20.7

Weight (kDa)

5.18

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mesd PF10185 71 - 174 1.4e-06 Chaperone for wingless signalling and trafficking of LDL receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 143
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 217
AccI GTMKAC 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 4 cut(s) 101, 109, 257, 322
AclWI GGATC 3 cut(s) 206, 532, 541
AcoI YGGCCR 1 cut(s) 339
AcyI GRCGYC 3 cut(s) 84, 141, 218
AfiI CCNNNNNNNGG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 3 cut(s) 430, 463, 478
AjnI CCWGG 3 cut(s) 132, 167, 529
AluBI AGCT 2 cut(s) 304, 346
AluI AGCT 2 cut(s) 304, 346
AlwI GGATC 3 cut(s) 206, 532, 541
Ama87I CYCGRG 1 cut(s) 327
Aor13HI TCCGGA 1 cut(s) 306
AoxI GGCC 4 cut(s) 281, 339, 442, 489
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 281, 375
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 115
AsuII TTCGAA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 532
BccI CCATC 2 cut(s) 229, 304
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 3 cut(s) 134, 169, 531
BciVI GTATCC 1 cut(s) 325
BcnI CCSGG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 221
BfuI GTATCC 1 cut(s) 325
Bme1390I CCNGG 4 cut(s) 134, 169, 280, 531
Bme18I GGWCC 1 cut(s) 375
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 2 cut(s) 281, 375
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 4 cut(s) 134, 169, 280, 531
BpiI GAAGAC 1 cut(s) 532
Bpu14I TTCGAA 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 280
Bsa29I ATCGAT 1 cut(s) 536
BsaBI GATNNNNATC 1 cut(s) 210
BsaHI GRCGYC 3 cut(s) 84, 141, 218
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 3 cut(s) 87, 306, 377
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 20
Bse8I GATNNNNATC 1 cut(s) 210
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 3 cut(s) 134, 169, 531
BseCI ATCGAT 1 cut(s) 536
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 315
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 4 cut(s) 23, 35, 38, 170
BshFI GGCC 4 cut(s) 283, 341, 444, 491
BshNI GGYRCC 1 cut(s) 217
BshVI ATCGAT 1 cut(s) 536
BsiHKCI CYCGRG 1 cut(s) 327
BsiSI CCGG 6 cut(s) 88, 215, 279, 284, 307, 378
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 4 cut(s) 283, 341, 444, 491
BsoBI CYCGRG 1 cut(s) 327
Bsp119I TTCGAA 1 cut(s) 523
Bsp13I TCCGGA 1 cut(s) 306
Bsp143I GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
BspACI CCGC 4 cut(s) 101, 109, 257, 322
BspANI GGCC 4 cut(s) 283, 341, 444, 491
BspCNI CTCAG 1 cut(s) 363
BspDI ATCGAT 1 cut(s) 536
BspEI TCCGGA 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 3 cut(s) 206, 532, 541
BspT104I TTCGAA 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 217
BsrFI RCCGGY 1 cut(s) 283
BsrI ACTGG 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
BssNI GRCGYC 3 cut(s) 84, 141, 218
Bst2UI CCWGG 3 cut(s) 134, 169, 531
Bst4CI ACNGT 1 cut(s) 337
Bst6I CTCTTC 1 cut(s) 205
BstACI GRCGYC 3 cut(s) 84, 141, 218
BstBI TTCGAA 1 cut(s) 523
BstC8I GCNNGC 2 cut(s) 285, 348
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 7 cut(s) 214, 226, 412, 423, 514, 536, 540
BstMBI GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
BstMWI GCNNNNNNNGC 3 cut(s) 280, 347, 389
BstNI CCWGG 3 cut(s) 134, 169, 531
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 4 cut(s) 132, 167, 278, 529
BstV2I GAAGAC 1 cut(s) 532
Bsu15I ATCGAT 1 cut(s) 536
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 4 cut(s) 283, 341, 444, 491
BsuTUI ATCGAT 1 cut(s) 536
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 2 cut(s) 285, 348
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 281, 375
ClaI ATCGAT 1 cut(s) 536
CseI GACGC 1 cut(s) 73
CviAII CATG 4 cut(s) 241, 400, 424, 433
CviJI RGCY 7 cut(s) 283, 287, 304, 341, 346, 444, 491
CviKI_1 RGCY 7 cut(s) 283, 287, 304, 341, 346, 444, 491
DdeI CTNAG 1 cut(s) 371
DinI GGCGCC 1 cut(s) 219
DpnI GATC 7 cut(s) 213, 225, 411, 422, 513, 535, 539
DpnII GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 272
Eco147I AGGCCT 1 cut(s) 491
Eco47I GGWCC 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 327
EcoRII CCWGG 3 cut(s) 132, 167, 529
EgeI GGCGCC 1 cut(s) 219
EheI GGCGCC 1 cut(s) 219
FaeI CATG 4 cut(s) 244, 403, 427, 436
FaiI YATR 5 cut(s) 242, 353, 401, 425, 434
FaqI GGGAC 1 cut(s) 8
FatI CATG 4 cut(s) 240, 399, 423, 432
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 1 cut(s) 147
FokI GGATG 1 cut(s) 322
GlaI GCGC 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 4 cut(s) 283, 341, 444, 491
HapII CCGG 6 cut(s) 88, 215, 279, 284, 307, 378
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 3 cut(s) 84, 141, 218
Hin1II CATG 4 cut(s) 244, 403, 427, 436
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 3 cut(s) 114, 325, 361
HpaII CCGG 6 cut(s) 88, 215, 279, 284, 307, 378
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 4 cut(s) 118, 148, 360, 471
Hpy188I TCNGA 2 cut(s) 205, 372
Hpy188III TCNNGA 3 cut(s) 298, 307, 329
Hpy8I GTNNAC 4 cut(s) 118, 148, 360, 471
Hpy99I CGWCG 5 cut(s) 131, 143, 146, 149, 152
HpyAV CCTTC 4 cut(s) 38, 50, 73, 457
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 2 cut(s) 141, 473
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 347, 389
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 2 cut(s) 141, 473
Hsp92I GRCGYC 3 cut(s) 84, 141, 218
Hsp92II CATG 4 cut(s) 244, 403, 427, 436
HspAI GCGC 1 cut(s) 218
KasI GGCGCC 1 cut(s) 217
Kpn2I TCCGGA 1 cut(s) 306
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Kzo9I GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
LmnI GCTCC 2 cut(s) 309, 380
MaeII ACGT 2 cut(s) 141, 473
MalI GATC 7 cut(s) 213, 225, 411, 422, 513, 535, 539
MboI GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
MboII GAAGA 3 cut(s) 192, 244, 537
MfeI CAATTG 1 cut(s) 384
MluCI AATT 1 cut(s) 384
Mly113I GGCGCC 1 cut(s) 218
MlyI GAGTC 3 cut(s) 123, 319, 355
MroI TCCGGA 1 cut(s) 306
MroNI GCCGGC 1 cut(s) 283
MslI CAYNNNNRTG 1 cut(s) 422
MspI CCGG 6 cut(s) 88, 215, 279, 284, 307, 378
MspR9I CCNGG 4 cut(s) 134, 169, 280, 531
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 3 cut(s) 134, 169, 531
MwoI GCNNNNNNNGC 3 cut(s) 280, 347, 389
NaeI GCCGGC 1 cut(s) 285
NarI GGCGCC 1 cut(s) 218
NciI CCSGG 1 cut(s) 280
NdeII GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 4 cut(s) 244, 403, 427, 436
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 2 cut(s) 121, 367
NspI RCATGY 1 cut(s) 244
NspV TTCGAA 1 cut(s) 523
PceI AGGCCT 1 cut(s) 491
PciI ACATGT 1 cut(s) 240
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 144, 147
PdiI GCCGGC 1 cut(s) 285
PflFI GACNNNGTC 2 cut(s) 140, 143
PleI GAGTC 3 cut(s) 122, 319, 355
PluTI GGCGCC 1 cut(s) 221
PpsI GAGTC 3 cut(s) 122, 319, 355
PscI ACATGT 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 132, 167, 529
PspGI CCWGG 3 cut(s) 132, 167, 529
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 2 cut(s) 281, 375
PsyI GACNNNGTC 2 cut(s) 140, 143
RseI CAYNNNNRTG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 146
Sau3AI GATC 7 cut(s) 211, 223, 409, 420, 511, 533, 537
Sau96I GGNCC 2 cut(s) 281, 375
SchI GAGTC 3 cut(s) 123, 319, 355
ScrFI CCNGG 4 cut(s) 134, 169, 280, 531
SetI ASST 8 cut(s) 19, 135, 144, 170, 306, 348, 398, 476
SfoI GGCGCC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 523
SgrDI CGTCGACG 1 cut(s) 146
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
Sse9I AATT 1 cut(s) 384
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 4 cut(s) 101, 109, 257, 322
SspDI GGCGCC 1 cut(s) 217
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 4 cut(s) 132, 167, 278, 529
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 2 cut(s) 144, 476
TaqI TCGA 5 cut(s) 147, 210, 230, 523, 536
TasI AATT 1 cut(s) 384
TscAI CASTG 1 cut(s) 342
TspDTI ATGAA 4 cut(s) 55, 281, 368, 388
TspRI CASTG 1 cut(s) 342
Tth111I GACNNNGTC 2 cut(s) 140, 143
VpaK11BI GGWCC 1 cut(s) 375
XceI RCATGY 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 147
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.