Rroxscaffold_4G00299520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007895
Physical Location & Seq
Forward (+)
19471585 .. 19473116
1532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299520.1

Sequence Viewer

Length: 564 bp
ATGTCAAACCCCAGAGCACCCTTTTCCTTCCTGGTCGCTGTTCTCGTTTTCTTTATTCTACCCCTCATCTTTTCCCAAAATGGCGGGTACGTCCGGTTCGCCGAGGGAGGAAAGCGGCGAGTCCACATCACCGACGACCTCGACGACGTCGTAGACGACGAAGAGGACGACACTTGGAAAGAATGGGGCAAGAAATCCACACAAACCTTCGATCCGCCGCCGAACGACTTGTCGAAGATGGAGTTATCGGAGATCCAGGCCGAGATGATGAAGCGGCATTCCGGGCCGGTTTTCGGGTTCATCAAGCTCCGACTCGGCGTCAAACGGACTCGGGATATGGTGGCTGAGATTGCTATGAAATGGAGTAAAGTTTTGAGAACAGGATCTATTGGGGTACGGTTCATGGGTGTTGATATGAGCACGATCATGGTCACCATGGAAAGAGGCCAAGACATGACAGAGTTGAAGGAGTTCGTGTTGAGCCAACCAGAGGCCTACGAGATAAAGATTGGAGATCACGTTTTTTCGAAGACCTGGAGATCCTCCTTTAGACCAAGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mesd

Protein Analysis

187

Amino Acids

21.49

Weight (kDa)

6.85

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 153
AciI CCGC 5 cut(s) 84, 115, 215, 218, 274
AclWI GGATC 4 cut(s) 206, 247, 391, 534
AcyI GRCGYC 2 cut(s) 147, 318
AfaI GTAC 2 cut(s) 89, 396
AfiI CCNNNNNNNGG 2 cut(s) 293, 490
AgsI TTSAA 1 cut(s) 466
AhdI GACNNNNNGTC 1 cut(s) 317
AjnI CCWGG 3 cut(s) 30, 255, 533
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 2 cut(s) 19, 422
AlwI GGATC 4 cut(s) 206, 247, 391, 534
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 4 cut(s) 258, 284, 445, 492
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
Asp700I GAANNNNTTC 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 121, 424
AsuII TTCGAA 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 19, 422
BccI CCATC 1 cut(s) 232
BciT130I CCWGG 3 cut(s) 32, 257, 535
BcnI CCSGG 1 cut(s) 283
BisI GCNGC 3 cut(s) 116, 218, 275
BlsI GCNGC 3 cut(s) 117, 219, 276
Bme1390I CCNGG 4 cut(s) 32, 257, 283, 535
BmeRI GACNNNNNGTC 1 cut(s) 317
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 284
BmrFI CCNGG 4 cut(s) 32, 257, 283, 535
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 556
Bpu14I TTCGAA 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 283
BsaHI GRCGYC 2 cut(s) 147, 318
BsaJI CCNNGG 2 cut(s) 102, 435
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 504, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 490
Bse118I RCCGGY 1 cut(s) 286
BseBI CCWGG 3 cut(s) 32, 257, 535
BseDI CCNNGG 2 cut(s) 102, 435
BseLI CCNNNNNNNGG 2 cut(s) 293, 490
BseMII CTCAG 1 cut(s) 336
BshFI GGCC 4 cut(s) 260, 286, 447, 494
BsiHKAI GWGCWC 2 cut(s) 19, 422
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 94, 282, 287
BslI CCNNNNNNNGG 2 cut(s) 293, 490
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 260, 286, 447, 494
BsoBI CYCGRG 1 cut(s) 330
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 19, 422
Bsp143I GATC 6 cut(s) 211, 252, 383, 423, 514, 539
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 5 cut(s) 84, 115, 215, 218, 274
BspANI GGCC 4 cut(s) 260, 286, 447, 494
BspCNI CTCAG 1 cut(s) 337
BspPI GGATC 4 cut(s) 206, 247, 391, 534
BspT104I TTCGAA 1 cut(s) 527
BsrFI RCCGGY 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 102, 435
BssMI GATC 6 cut(s) 211, 252, 383, 423, 514, 539
BssNI GRCGYC 2 cut(s) 147, 318
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 3 cut(s) 32, 257, 535
Bst4CI ACNGT 1 cut(s) 399
Bst6I CTCTTC 1 cut(s) 156
BstACI GRCGYC 2 cut(s) 147, 318
BstBI TTCGAA 1 cut(s) 527
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 1 cut(s) 435
BstEII GGTNACC 1 cut(s) 430
BstKTI GATC 6 cut(s) 214, 255, 386, 426, 517, 542
BstMBI GATC 6 cut(s) 211, 252, 383, 423, 514, 539
BstMWI GCNNNNNNNGC 2 cut(s) 283, 350
BstNI CCWGG 3 cut(s) 32, 257, 535
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 4 cut(s) 30, 255, 281, 533
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 3 cut(s) 252, 383, 539
BstYI RGATCY 3 cut(s) 252, 383, 539
BsuRI GGCC 4 cut(s) 260, 286, 447, 494
BtgI CCRYGG 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 284
CseI GACGC 1 cut(s) 307
Csp6I GTAC 2 cut(s) 88, 395
CviAII CATG 4 cut(s) 403, 427, 436, 454
CviJI RGCY 7 cut(s) 260, 286, 307, 344, 447, 483, 494
CviKI_1 RGCY 7 cut(s) 260, 286, 307, 344, 447, 483, 494
CviQI GTAC 2 cut(s) 88, 395
DdeI CTNAG 1 cut(s) 345
DpnI GATC 6 cut(s) 213, 254, 385, 425, 516, 541
DpnII GATC 6 cut(s) 211, 252, 383, 423, 514, 539
DriI GACNNNNNGTC 1 cut(s) 317
Eam1104I CTCTTC 1 cut(s) 156
Eam1105I GACNNNNNGTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 156
EciI GGCGGA 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 435
Eco147I AGGCCT 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 330
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 30, 255, 533
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 4 cut(s) 406, 430, 439, 457
FaiI YATR 7 cut(s) 338, 356, 404, 416, 428, 437, 455
FatI CATG 4 cut(s) 402, 426, 435, 453
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 116, 218, 275
Fsp4HI GCNGC 3 cut(s) 116, 218, 275
GluI GCNGC 3 cut(s) 116, 218, 275
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 4 cut(s) 260, 286, 447, 494
HapII CCGG 3 cut(s) 94, 282, 287
HgaI GACGC 1 cut(s) 307
Hin1I GRCGYC 2 cut(s) 147, 318
Hin1II CATG 4 cut(s) 406, 430, 439, 457
HinfI GANTC 3 cut(s) 120, 312, 328
HpaII CCGG 3 cut(s) 94, 282, 287
HphI GGTGA 2 cut(s) 121, 424
Hpy166II GTNNAC 2 cut(s) 124, 154
Hpy188I TCNGA 2 cut(s) 250, 311
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 124, 154
Hpy99I CGWCG 5 cut(s) 137, 146, 149, 152, 161
HpyAV CCTTC 3 cut(s) 37, 217, 460
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4IV ACGT 3 cut(s) 90, 147, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 350
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 3 cut(s) 90, 147, 519
Hsp92I GRCGYC 2 cut(s) 147, 318
Hsp92II CATG 4 cut(s) 406, 430, 439, 457
Kzo9I GATC 6 cut(s) 211, 252, 383, 423, 514, 539
LmnI GCTCC 1 cut(s) 312
MaeII ACGT 3 cut(s) 90, 147, 519
MaeIII GTNAC 1 cut(s) 430
MalI GATC 6 cut(s) 213, 254, 385, 425, 516, 541
MboI GATC 6 cut(s) 211, 252, 383, 423, 514, 539
MboII GAAGA 3 cut(s) 173, 247, 541
MflI RGATCY 3 cut(s) 252, 383, 539
MhlI GDGCHC 2 cut(s) 19, 422
MlyI GAGTC 3 cut(s) 129, 306, 322
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 8 cut(s) 74, 97, 101, 149, 157, 437, 484, 553
MroXI GAANNNNTTC 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 3 cut(s) 94, 282, 287
MspR9I CCNGG 4 cut(s) 32, 257, 283, 535
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 3 cut(s) 32, 257, 535
MwoI GCNNNNNNNGC 2 cut(s) 283, 350
NciI CCSGG 1 cut(s) 283
NcoI CCATGG 1 cut(s) 435
NdeII GATC 6 cut(s) 211, 252, 383, 423, 514, 539
NlaIII CATG 4 cut(s) 406, 430, 439, 457
NmeAIII GCCGAG 3 cut(s) 127, 286, 294
NmuCI GTSAC 1 cut(s) 430
NspV TTCGAA 1 cut(s) 527
PceI AGGCCT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 6 cut(s) 42, 141, 147, 153, 156, 165
PctI GAATGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 470
PflFI GACNNNGTC 2 cut(s) 146, 555
PkrI GCNGC 3 cut(s) 117, 219, 276
PleI GAGTC 3 cut(s) 128, 306, 322
PpsI GAGTC 3 cut(s) 128, 306, 322
Psp6I CCWGG 3 cut(s) 30, 255, 533
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 3 cut(s) 30, 255, 533
PspPI GGNCC 1 cut(s) 284
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PsuI RGATCY 3 cut(s) 252, 383, 539
PsyI GACNNNGTC 2 cut(s) 146, 555
RsaI GTAC 2 cut(s) 89, 396
RsaNI GTAC 2 cut(s) 88, 395
RseI CAYNNNNRTG 1 cut(s) 425
SatI GCNGC 3 cut(s) 116, 218, 275
Sau3AI GATC 6 cut(s) 211, 252, 383, 423, 514, 539
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 3 cut(s) 129, 306, 322
ScrFI CCNGG 4 cut(s) 32, 257, 283, 535
SduI GDGCHC 2 cut(s) 19, 422
SetI ASST 7 cut(s) 93, 141, 150, 209, 309, 522, 536
SfuI TTCGAA 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 425
SseBI AGGCCT 1 cut(s) 494
SsiI CCGC 5 cut(s) 84, 115, 215, 218, 274
StuI AGGCCT 1 cut(s) 494
StyD4I CCNGG 4 cut(s) 30, 255, 281, 533
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 399
TaiI ACGT 3 cut(s) 93, 150, 522
TaqI TCGA 4 cut(s) 141, 210, 233, 527
TauI GCSGC 3 cut(s) 118, 220, 277
TseFI GTSAC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 4 cut(s) 284, 289, 371, 391
TspGWI ACGGA 1 cut(s) 340
Tth111I GACNNNGTC 2 cut(s) 146, 555
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 470
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.