Rroxscaffold_4G00299740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007895
Physical Location & Seq
Forward (+)
19694051 .. 19695606
1556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299740.1

Sequence Viewer

Length: 642 bp
ATGCCAAACCCCAGAGCACCCTTTCCCTTCCTCGTCACCGTTCTTGTTTTCTTTATTCTACCCCTCATCTTTTTCCCAAATGGCGGGTACGTCCGGTTCGCCGAGGGAGGAAAGCGGCGAGTCCACATCACCGACGACCTCGACGACGTCGTAGACGACGAAGAGGACGACACTTGGAAAAAATGGGGCAAGAAATCCACACAAACCTTCGATCCGCCGCCGAACGACTTGTCGAAGATGGAGTTATCGGAGATCCAGGCCGAGATGATGAAGCGGCATTCCGGGCCGGTTTTCGGGTTCATCAAGCTCCGACTCGGCGTTAAACGGACTCGGGATATGGTGGCTGAGATTGCTATGAAATGGAGTAAAGTTCTAAGAACTGGATCAATTGGGGTACGGTTCATGGGTGTTGATATGAGCACGATCATGGTCACCATGGAAAGAGGCCAAGACATGACAGAGTTGAAGGAGTTCGTGTTGAGCCAACCAGAGGCCTACGAGATAAAGATTGGAGATCAAGTTTTTCGAAGACCTGGAGATCCTCCTTTAGACCAAGTCGTTGAGAAGCTCCAGAATGAGAGAAAGAGCATCGAGAATGACGGTTCAACAGAGAGCAATAGGCAAGTGAAAGAGGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mesd

Protein Analysis

213

Amino Acids

24.45

Weight (kDa)

5.42

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 153
AciI CCGC 5 cut(s) 84, 115, 215, 218, 274
AclWI GGATC 4 cut(s) 206, 247, 391, 533
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 2 cut(s) 89, 396
AfiI CCNNNNNNNGG 3 cut(s) 83, 293, 490
AgsI TTSAA 2 cut(s) 466, 606
AjnI CCWGG 2 cut(s) 255, 532
AluBI AGCT 2 cut(s) 307, 568
AluI AGCT 2 cut(s) 307, 568
Alw21I GWGCWC 2 cut(s) 19, 422
AlwI GGATC 4 cut(s) 206, 247, 391, 533
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 4 cut(s) 258, 284, 445, 492
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
Asp700I GAANNNNTTC 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 283
AsuHPI GGTGA 3 cut(s) 28, 121, 424
AsuII TTCGAA 1 cut(s) 526
AvaI CYCGRG 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 535
Bbv12I GWGCWC 2 cut(s) 19, 422
BccI CCATC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 257, 534
BcnI CCSGG 1 cut(s) 283
BfmI CTRYAG 1 cut(s) 638
BisI GCNGC 3 cut(s) 116, 218, 275
BlsI GCNGC 3 cut(s) 117, 219, 276
Bme1390I CCNGG 3 cut(s) 257, 283, 534
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 284
BmrFI CCNGG 3 cut(s) 257, 283, 534
BmsI GCATC 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 535
BpmI CTGGAG 2 cut(s) 554, 555
Bpu14I TTCGAA 1 cut(s) 526
BpuMI CCSGG 1 cut(s) 283
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 102, 435
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 293, 490
Bse118I RCCGGY 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 385
BseBI CCWGG 2 cut(s) 257, 534
BseDI CCNNGG 2 cut(s) 102, 435
BseLI CCNNNNNNNGG 3 cut(s) 83, 293, 490
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 385
BshFI GGCC 4 cut(s) 260, 286, 447, 494
BsiHKAI GWGCWC 2 cut(s) 19, 422
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 94, 282, 287
BslI CCNNNNNNNGG 3 cut(s) 83, 293, 490
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 260, 286, 447, 494
BsoBI CYCGRG 1 cut(s) 330
Bsp119I TTCGAA 1 cut(s) 526
Bsp1286I GDGCHC 2 cut(s) 19, 422
Bsp143I GATC 6 cut(s) 211, 252, 383, 423, 514, 538
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 5 cut(s) 84, 115, 215, 218, 274
BspANI GGCC 4 cut(s) 260, 286, 447, 494
BspCNI CTCAG 1 cut(s) 337
BspPI GGATC 4 cut(s) 206, 247, 391, 533
BspT104I TTCGAA 1 cut(s) 526
BsrFI RCCGGY 1 cut(s) 286
BsrI ACTGG 1 cut(s) 385
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 102, 435
BssMI GATC 6 cut(s) 211, 252, 383, 423, 514, 538
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 257, 534
Bst4CI ACNGT 4 cut(s) 40, 399, 602, 639
Bst6I CTCTTC 1 cut(s) 156
BstACI GRCGYC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 526
BstDEI CTNAG 2 cut(s) 345, 374
BstDSI CCRYGG 1 cut(s) 435
BstEII GGTNACC 1 cut(s) 430
BstKTI GATC 6 cut(s) 214, 255, 386, 426, 517, 541
BstMBI GATC 6 cut(s) 211, 252, 383, 423, 514, 538
BstMWI GCNNNNNNNGC 2 cut(s) 283, 350
BstNI CCWGG 2 cut(s) 257, 534
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 255, 281, 532
BstSFI CTRYAG 1 cut(s) 638
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 2 cut(s) 252, 538
BstYI RGATCY 2 cut(s) 252, 538
BsuRI GGCC 4 cut(s) 260, 286, 447, 494
BtgI CCRYGG 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 88, 395
CviAII CATG 4 cut(s) 403, 427, 436, 454
CviJI RGCY 8 cut(s) 260, 286, 307, 344, 447, 483, 494, 568
CviKI_1 RGCY 8 cut(s) 260, 286, 307, 344, 447, 483, 494, 568
CviQI GTAC 2 cut(s) 88, 395
DdeI CTNAG 2 cut(s) 345, 374
DpnI GATC 6 cut(s) 213, 254, 385, 425, 516, 540
DpnII GATC 6 cut(s) 211, 252, 383, 423, 514, 538
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
EciI GGCGGA 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 435
Eco147I AGGCCT 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 330
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 255, 532
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 4 cut(s) 406, 430, 439, 457
FaiI YATR 7 cut(s) 338, 356, 404, 416, 428, 437, 455
FatI CATG 4 cut(s) 402, 426, 435, 453
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 116, 218, 275
Fsp4HI GCNGC 3 cut(s) 116, 218, 275
GluI GCNGC 3 cut(s) 116, 218, 275
GsuI CTGGAG 2 cut(s) 554, 555
HaeIII GGCC 4 cut(s) 260, 286, 447, 494
HapII CCGG 3 cut(s) 94, 282, 287
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 406, 430, 439, 457
HinfI GANTC 3 cut(s) 120, 312, 328
HpaII CCGG 3 cut(s) 94, 282, 287
HphI GGTGA 3 cut(s) 28, 121, 424
Hpy166II GTNNAC 2 cut(s) 124, 154
Hpy188I TCNGA 2 cut(s) 250, 311
Hpy188III TCNNGA 3 cut(s) 332, 571, 592
Hpy8I GTNNAC 2 cut(s) 124, 154
Hpy99I CGWCG 5 cut(s) 137, 146, 149, 152, 161
HpyAV CCTTC 3 cut(s) 37, 217, 460
HpyCH4III ACNGT 4 cut(s) 40, 399, 602, 639
HpyCH4IV ACGT 2 cut(s) 90, 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 350
HpyF3I CTNAG 2 cut(s) 345, 374
HpySE526I ACGT 2 cut(s) 90, 147
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 406, 430, 439, 457
Kzo9I GATC 6 cut(s) 211, 252, 383, 423, 514, 538
LmnI GCTCC 2 cut(s) 312, 573
LweI GCATC 1 cut(s) 597
MaeII ACGT 2 cut(s) 90, 147
MaeIII GTNAC 2 cut(s) 34, 430
MalI GATC 6 cut(s) 213, 254, 385, 425, 516, 540
MboI GATC 6 cut(s) 211, 252, 383, 423, 514, 538
MboII GAAGA 3 cut(s) 173, 247, 540
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 2 cut(s) 252, 538
MhlI GDGCHC 2 cut(s) 19, 422
MluCI AATT 1 cut(s) 387
MlyI GAGTC 3 cut(s) 129, 306, 322
MmeI TCCRAC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 3 cut(s) 94, 282, 287
MspR9I CCNGG 3 cut(s) 257, 283, 534
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 2 cut(s) 257, 534
MwoI GCNNNNNNNGC 2 cut(s) 283, 350
NciI CCSGG 1 cut(s) 283
NcoI CCATGG 1 cut(s) 435
NdeII GATC 6 cut(s) 211, 252, 383, 423, 514, 538
NlaIII CATG 4 cut(s) 406, 430, 439, 457
NmeAIII GCCGAG 3 cut(s) 127, 286, 294
NmuCI GTSAC 2 cut(s) 34, 430
NspV TTCGAA 1 cut(s) 526
PceI AGGCCT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 5 cut(s) 141, 147, 153, 156, 165
PctI GAATGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 470
PflFI GACNNNGTC 2 cut(s) 146, 554
PkrI GCNGC 3 cut(s) 117, 219, 276
PleI GAGTC 3 cut(s) 128, 306, 322
PpsI GAGTC 3 cut(s) 128, 306, 322
Psp6I CCWGG 2 cut(s) 255, 532
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 2 cut(s) 255, 532
PspPI GGNCC 1 cut(s) 284
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PsuI RGATCY 2 cut(s) 252, 538
PsyI GACNNNGTC 2 cut(s) 146, 554
RsaI GTAC 2 cut(s) 89, 396
RsaNI GTAC 2 cut(s) 88, 395
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 116, 218, 275
Sau3AI GATC 6 cut(s) 211, 252, 383, 423, 514, 538
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 3 cut(s) 129, 306, 322
ScrFI CCNGG 3 cut(s) 257, 283, 534
SduI GDGCHC 2 cut(s) 19, 422
SetI ASST 7 cut(s) 93, 141, 150, 209, 309, 535, 570
SfaNI GCATC 1 cut(s) 597
SfcI CTRYAG 1 cut(s) 638
SfuI TTCGAA 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 1 cut(s) 387
SseBI AGGCCT 1 cut(s) 494
SsiI CCGC 5 cut(s) 84, 115, 215, 218, 274
StuI AGGCCT 1 cut(s) 494
StyD4I CCNGG 3 cut(s) 255, 281, 532
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 4 cut(s) 40, 399, 602, 639
TaiI ACGT 2 cut(s) 93, 150
TaqI TCGA 5 cut(s) 141, 210, 233, 526, 591
TasI AATT 1 cut(s) 387
TauI GCSGC 3 cut(s) 118, 220, 277
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseFI GTSAC 2 cut(s) 34, 430
Tsp45I GTSAC 2 cut(s) 34, 430
TspDTI ATGAA 4 cut(s) 284, 289, 371, 391
TspGWI ACGGA 1 cut(s) 340
Tth111I GACNNNGTC 2 cut(s) 146, 554
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 470
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.