MD01G1074800.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002732
Physical Location & Seq
Forward (+)
18104343 .. 18108055
3713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1074800.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGGCCATTCAGCAGGAGAATGGGAACATAGGGCAGCAATTGAGATTACAAAAGATTGGAATGGGGTAGAACAAGTTGTGCTTCAGAACCAGCAAGGGGCTTCGGCACGGGTTAGCTTACTTGGAGGACAGGTCACTTCATGGAGGAATAAGCACGGCGAAGAACTCCTGTTCACTAGCAGCAAGGCAATCTTCAAGCCCCCAAAAGCGATGCGAGGAGGAATTCCCATTTGTTTCCCACAGTTTGGGAACTGTGGATCGCTGGAAAAGCATGGTTTTGCTAGGAATAAGGTGTGGGCAATTGATGAGAATCCTGAAATGCGTCAACCAAATGATTCCAATGGAAAATCCTTTATTGATCTACTACTAAAACCGTCTGAAGACGACCTGAAGTGTTGGCCTCATAGTTTTGAGTTTCGACTTAGGGTGTCTCTTGCAACAAATGGAGATCTGATCCTGACATCACGAGTTAGAAATGTCAACGGCAAGCCGTTTGATTTCTCCTTTGCCTACCACACTTATTTTTTGGTTTCTGATATAAGTGAGGTGAGGATAGAAGGGCTGGAGACACTTGACTACTTAGACAACCTTTGTGAACGACAACGTTTTACAGAACAAGGAGATGCCATAACTTTTGAGTCTGAGGTCAGTCGACTATATCTAAGTTCGCCCAATGTAGTTGCAGTCCTTGATCATGAGAAAAAGCGAACATATGTAATCCGAAAGGAAGGACTACCAGATGTTGTTGTTTGGAATCCGTGGGAGAAGAAATCGAAATCTATGGTTGATTTTGGTGATGAGGAGTACAAGCAGATGCTCTGTGTGGATGGAGCAGCGATAGAGAAACCTATCACATTGAAGCCAGGCAAGGAATGGACAGGGCGACTGCAGCTCTCGGTTGTGCCTTCGAGTTTCTGCAGCGAGCACCTTGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

313

Amino Acids

35.4

Weight (kDa)

5.82

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 25 - 298 4.9e-68 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 245
AccI GTMKAC 1 cut(s) 652
AclI AACGTT 1 cut(s) 604
AclWI GGATC 2 cut(s) 265, 448
AcsI RAATTY 1 cut(s) 222
AcuI CTGAAG 3 cut(s) 68, 399, 410
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 2 cut(s) 97, 245
AgsI TTSAA 2 cut(s) 196, 859
AjnI CCWGG 1 cut(s) 862
AluBI AGCT 2 cut(s) 117, 892
AluI AGCT 2 cut(s) 117, 892
Alw21I GWGCWC 1 cut(s) 927
Alw26I GTCTC 2 cut(s) 435, 560
AlwI GGATC 2 cut(s) 265, 448
AoxI GGCC 2 cut(s) 4, 398
ApeKI GCWGC 5 cut(s) 35, 180, 833, 889, 918
ApoI RAATTY 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 559, 806
BauI CACGAG 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 927
BbvI GCAGC 5 cut(s) 47, 192, 845, 901, 930
BccI CCATC 1 cut(s) 821
BceAI ACGGC 3 cut(s) 172, 475, 499
BciT130I CCWGG 1 cut(s) 864
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 435, 560
BfaI CTAG 2 cut(s) 177, 282
BfmI CTRYAG 2 cut(s) 887, 916
BglII AGATCT 1 cut(s) 448
BisI GCNGC 5 cut(s) 36, 181, 834, 890, 919
BlsI GCNGC 5 cut(s) 37, 182, 835, 891, 920
Bme1390I CCNGG 1 cut(s) 864
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 864
BmsI GCATC 3 cut(s) 201, 613, 804
BpiI GAAGAC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 584
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 758
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 245
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 1 cut(s) 758
BseGI GGATG 1 cut(s) 832
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 97, 245
BseMII CTCAG 1 cut(s) 633
BseRI GAGGAG 2 cut(s) 231, 815
BseXI GCAGC 5 cut(s) 47, 192, 845, 901, 930
BshFI GGCC 2 cut(s) 6, 400
BsiHKAI GWGCWC 1 cut(s) 927
BslI CCNNNNNNNGG 2 cut(s) 97, 245
BsmAI GTCTC 2 cut(s) 435, 560
BsnI GGCC 2 cut(s) 6, 400
Bsp1286I GDGCHC 1 cut(s) 927
Bsp143I GATC 6 cut(s) 257, 358, 448, 453, 691, 931
BspANI GGCC 2 cut(s) 6, 400
BspCNI CTCAG 1 cut(s) 634
BspHI TCATGA 1 cut(s) 694
BspMAI CTGCAG 2 cut(s) 891, 920
BspPI GGATC 2 cut(s) 265, 448
BssECI CCNNGG 1 cut(s) 758
BssMI GATC 6 cut(s) 257, 358, 448, 453, 691, 931
BssSI CACGAG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 3 cut(s) 243, 254, 375
BstC8I GCNNGC 2 cut(s) 488, 923
BstDEI CTNAG 4 cut(s) 422, 580, 642, 662
BstDSI CCRYGG 1 cut(s) 758
BstF5I GGATG 1 cut(s) 832
BstKTI GATC 6 cut(s) 260, 361, 451, 456, 694, 934
BstMAI GTCTC 2 cut(s) 435, 560
BstMBI GATC 6 cut(s) 257, 358, 448, 453, 691, 931
BstMWI GCNNNNNNNGC 2 cut(s) 268, 889
BstNI CCWGG 1 cut(s) 864
BstSCI CCNGG 1 cut(s) 862
BstSFI CTRYAG 2 cut(s) 887, 916
BstV1I GCAGC 5 cut(s) 47, 192, 845, 901, 930
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 2 cut(s) 6, 400
BtgI CCRYGG 1 cut(s) 758
BtgZI GCGATG 1 cut(s) 224
BtsCI GGATG 1 cut(s) 832
Cac8I GCNNGC 2 cut(s) 488, 923
CciI TCATGA 1 cut(s) 694
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 805
CviAII CATG 3 cut(s) 141, 272, 695
CviJI RGCY 9 cut(s) 6, 101, 117, 199, 400, 490, 562, 862, 892
CviKI_1 RGCY 9 cut(s) 6, 101, 117, 199, 400, 490, 562, 862, 892
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 4 cut(s) 422, 580, 642, 662
DpnI GATC 6 cut(s) 259, 360, 450, 455, 693, 933
DpnII GATC 6 cut(s) 257, 358, 448, 453, 691, 931
Eco57I CTGAAG 3 cut(s) 68, 399, 410
EcoRI GAATTC 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 862
FaeI CATG 3 cut(s) 144, 275, 698
FalI AAGNNNNNCTT 4 cut(s) 66, 98, 176, 208
FatI CATG 3 cut(s) 140, 271, 694
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 5 cut(s) 36, 181, 834, 890, 919
FokI GGATG 1 cut(s) 839
Fsp4HI GCNGC 5 cut(s) 36, 181, 834, 890, 919
FspBI CTAG 2 cut(s) 177, 282
GluI GCNGC 5 cut(s) 36, 181, 834, 890, 919
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 2 cut(s) 6, 400
HgaI GACGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 144, 275, 698
HincII GTYRAC 3 cut(s) 326, 481, 653
HindII GTYRAC 3 cut(s) 326, 481, 653
HinfI GANTC 4 cut(s) 310, 335, 638, 754
HphI GGTGA 2 cut(s) 559, 806
Hpy166II GTNNAC 5 cut(s) 174, 326, 481, 596, 653
Hpy188I TCNGA 6 cut(s) 87, 379, 453, 535, 643, 722
Hpy188III TCNNGA 4 cut(s) 314, 457, 465, 695
Hpy8I GTNNAC 5 cut(s) 174, 326, 481, 596, 653
HpyAV CCTTC 3 cut(s) 551, 722, 915
HpyCH4III ACNGT 3 cut(s) 243, 254, 375
HpyCH4IV ACGT 1 cut(s) 604
HpyCH4V TGCA 4 cut(s) 437, 683, 889, 918
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 889
HpyF3I CTNAG 4 cut(s) 422, 580, 642, 662
HpySE526I ACGT 1 cut(s) 604
Hsp92II CATG 3 cut(s) 144, 275, 698
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 6 cut(s) 257, 358, 448, 453, 691, 931
LmnI GCTCC 1 cut(s) 830
Lsp1109I GCAGC 5 cut(s) 47, 192, 845, 901, 930
LweI GCATC 3 cut(s) 201, 613, 804
MaeI CTAG 2 cut(s) 177, 282
MaeII ACGT 1 cut(s) 604
MaeIII GTNAC 1 cut(s) 133
MalI GATC 6 cut(s) 259, 360, 450, 455, 693, 933
MboI GATC 6 cut(s) 257, 358, 448, 453, 691, 931
MboII GAAGA 4 cut(s) 173, 184, 392, 778
MfeI CAATTG 2 cut(s) 39, 300
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 927
MluCI AATT 3 cut(s) 39, 222, 300
MlyI GAGTC 1 cut(s) 647
MnlI CCTC 9 cut(s) 119, 138, 209, 212, 411, 538, 543, 637, 793
MspR9I CCNGG 1 cut(s) 864
MunI CAATTG 2 cut(s) 39, 300
MvaI CCWGG 1 cut(s) 864
MwoI GCNNNNNNNGC 2 cut(s) 268, 889
NdeI CATATG 1 cut(s) 712
NdeII GATC 6 cut(s) 257, 358, 448, 453, 691, 931
NlaIII CATG 3 cut(s) 144, 275, 698
NmuCI GTSAC 1 cut(s) 133
PagI TCATGA 1 cut(s) 694
PfeI GAWTC 3 cut(s) 310, 335, 754
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 5 cut(s) 37, 182, 835, 891, 920
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
Psp1406I AACGTT 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 862
PspGI CCWGG 1 cut(s) 862
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 2 cut(s) 891, 920
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
SalI GTCGAC 1 cut(s) 651
SatI GCNGC 5 cut(s) 36, 181, 834, 890, 919
Sau3AI GATC 6 cut(s) 257, 358, 448, 453, 691, 931
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 1 cut(s) 864
SduI GDGCHC 1 cut(s) 927
SfaNI GCATC 3 cut(s) 201, 613, 804
SfcI CTRYAG 2 cut(s) 887, 916
Sse9I AATT 3 cut(s) 39, 222, 300
SspMI CTAG 2 cut(s) 177, 282
StyD4I CCNGG 1 cut(s) 862
TaaI ACNGT 3 cut(s) 243, 254, 375
TaiI ACGT 1 cut(s) 607
TaqI TCGA 4 cut(s) 418, 652, 773, 908
TasI AATT 3 cut(s) 39, 222, 300
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 3 cut(s) 310, 335, 754
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 5 cut(s) 35, 180, 833, 889, 918
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 1 cut(s) 747
Van91I CCANNNNNTGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 222
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XmiI GTMKAC 1 cut(s) 652
XspI CTAG 2 cut(s) 177, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.