MD07G1143900.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002732
Physical Location & Seq
Forward (+)
20983821 .. 20986920
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1143900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGGGCATTCAGCCGCAGAATGTCAATACAGGGCAGCAATTGAGATTACAAAAGATAGGAATGGGGTAGAACAGGTAGTGCTCCAGAACCAACAAGGGGCTTCGGCACGGGTTAGCTTACATGGAGGACAGGTCACTTCGTGGAGGAATAAGCACGGCCAAGAACTCCTGTTCACTAGTAACAAGGCAATCTTCAAGCCCGCAAAAGCGATGCGAGGAGGAATTGTCATTTGTTTCCCACAGTTTGGGAACTGTGGATCGCTAGAACAACATGGTTTTGCGAGGAACAAGGTGTGGGCAATTGATGATAATCCTGAAATGCATCAACCAAACGATTCGAATGGAAAATCCTTCATTGATCTACTACTTAAACCGTCTGAAAACGACCTGAAGTGTTGGCCCCATAGTTTTGAGTTTCGACTTAGGGTGTCTCTTGCAACAAATGGAGATCTGAACCTGACATCACGAGTTAGAAATGTCAACAGCAAGCCATTTAGTTTCTCCTTTGCCTACCACACTTATTTTTCGGTTTCTGATATAAGTGAGGTGAGGATAGAGGGGCTGGAGACACTTGACTACTTAGACAACCTTTGTGAAAGAAAATGCTTTACAGAACAAGGAGACGCCATAACATTTGAGTCTGAGGTCAGTCGACTTTATCTAAGTTCGCCCAATGTAGTTGCAGTCCTTGATCATGAGAAAAAACGAACATATGTAATCAGAAAGGAAGGACTACCAGATGTTGGTAAGCTGGCACATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

255

Amino Acids

28.59

Weight (kDa)

7.11

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 25 - 244 5.5e-49 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 245, 743
AccI GTMKAC 1 cut(s) 652
AciI CCGC 2 cut(s) 15, 201
AclWI GGATC 1 cut(s) 265
AcoI YGGCCR 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 410
AcyI GRCGYC 1 cut(s) 624
AdeI CACNNNGTG 1 cut(s) 141
AfiI CCNNNNNNNGG 3 cut(s) 97, 245, 743
AgsI TTSAA 1 cut(s) 196
AhlI ACTAGT 1 cut(s) 176
AluBI AGCT 2 cut(s) 117, 751
AluI AGCT 2 cut(s) 117, 751
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 3 cut(s) 435, 560, 615
AlwI GGATC 1 cut(s) 265
AoxI GGCC 2 cut(s) 157, 398
ApeKI GCWGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 559
AsuII TTCGAA 1 cut(s) 338
BauI CACGAG 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 47
BceAI ACGGC 1 cut(s) 172
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 3 cut(s) 435, 560, 615
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 2 cut(s) 177, 263
BglII AGATCT 1 cut(s) 448
BisI GCNGC 2 cut(s) 15, 36
BlsI GCNGC 2 cut(s) 16, 37
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 1 cut(s) 401
BmsI GCATC 2 cut(s) 201, 331
BpmI CTGGAG 2 cut(s) 68, 584
Bpu14I TTCGAA 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 624
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 210, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 245, 743
Bse8I GATNNNNATC 1 cut(s) 309
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 3 cut(s) 97, 245, 743
BseMII CTCAG 1 cut(s) 633
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 47
BshFI GGCC 2 cut(s) 159, 400
BsiHKAI GWGCWC 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 97, 245, 743
BsmAI GTCTC 3 cut(s) 435, 560, 615
BsmBI CGTCTC 1 cut(s) 615
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 2 cut(s) 159, 400
Bsp119I TTCGAA 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 4 cut(s) 257, 358, 448, 691
BspACI CCGC 2 cut(s) 15, 201
BspANI GGCC 2 cut(s) 159, 400
BspCNI CTCAG 1 cut(s) 634
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 401
BspPI GGATC 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 338
BssMI GATC 4 cut(s) 257, 358, 448, 691
BssNI GRCGYC 1 cut(s) 624
BssSI CACGAG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 465
Bst4CI ACNGT 3 cut(s) 243, 254, 375
BstACI GRCGYC 1 cut(s) 624
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 201, 488, 753
BstDEI CTNAG 4 cut(s) 422, 580, 642, 662
BstKTI GATC 4 cut(s) 260, 361, 451, 694
BstMAI GTCTC 3 cut(s) 435, 560, 615
BstMBI GATC 4 cut(s) 257, 358, 448, 691
BstV1I GCAGC 1 cut(s) 47
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 2 cut(s) 159, 400
BtgZI GCGATG 1 cut(s) 224
Cac8I GCNNGC 3 cut(s) 201, 488, 753
CciI TCATGA 1 cut(s) 694
Cfr13I GGNCC 1 cut(s) 399
CseI GACGC 1 cut(s) 632
CviAII CATG 3 cut(s) 122, 272, 695
CviJI RGCY 9 cut(s) 14, 101, 117, 159, 199, 400, 490, 562, 751
CviKI_1 RGCY 9 cut(s) 14, 101, 117, 159, 199, 400, 490, 562, 751
DdeI CTNAG 4 cut(s) 422, 580, 642, 662
DpnI GATC 4 cut(s) 259, 360, 450, 693
DpnII GATC 4 cut(s) 257, 358, 448, 691
DraIII CACNNNGTG 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 410
EcoT22I ATGCAT 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 615
FaeI CATG 3 cut(s) 125, 275, 698
FaiI YATR 9 cut(s) 123, 273, 405, 539, 629, 696, 712, 714, 759
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FatI CATG 3 cut(s) 121, 271, 694
FauI CCCGC 1 cut(s) 208
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 2 cut(s) 15, 36
Fsp4HI GCNGC 2 cut(s) 15, 36
FspBI CTAG 2 cut(s) 177, 263
GluI GCNGC 2 cut(s) 15, 36
GsuI CTGGAG 2 cut(s) 68, 584
HaeIII GGCC 2 cut(s) 159, 400
HgaI GACGC 1 cut(s) 632
Hin1I GRCGYC 1 cut(s) 624
Hin1II CATG 3 cut(s) 125, 275, 698
HincII GTYRAC 2 cut(s) 481, 653
HindII GTYRAC 2 cut(s) 481, 653
HinfI GANTC 2 cut(s) 335, 638
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 3 cut(s) 174, 481, 653
Hpy188I TCNGA 5 cut(s) 379, 453, 535, 643, 722
Hpy188III TCNNGA 4 cut(s) 85, 314, 465, 695
Hpy8I GTNNAC 3 cut(s) 174, 481, 653
HpyAV CCTTC 2 cut(s) 361, 722
HpyCH4III ACNGT 3 cut(s) 243, 254, 375
HpyCH4V TGCA 3 cut(s) 322, 437, 683
HpyF3I CTNAG 4 cut(s) 422, 580, 642, 662
Hsp92I GRCGYC 1 cut(s) 624
Hsp92II CATG 3 cut(s) 125, 275, 698
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 4 cut(s) 257, 358, 448, 691
LmnI GCTCC 1 cut(s) 87
Lsp1109I GCAGC 1 cut(s) 47
LweI GCATC 2 cut(s) 201, 331
MaeI CTAG 2 cut(s) 177, 263
MaeIII GTNAC 2 cut(s) 133, 179
MalI GATC 4 cut(s) 259, 360, 450, 693
MboI GATC 4 cut(s) 257, 358, 448, 691
MboII GAAGA 1 cut(s) 184
MfeI CAATTG 2 cut(s) 39, 300
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 3 cut(s) 39, 222, 300
MlyI GAGTC 1 cut(s) 647
MnlI CCTC 9 cut(s) 119, 138, 209, 212, 276, 538, 543, 550, 637
Mph1103I ATGCAT 1 cut(s) 324
MseI TTAA 1 cut(s) 369
MunI CAATTG 2 cut(s) 39, 300
Mva1269I GAATGC 1 cut(s) 7
NdeI CATATG 1 cut(s) 712
NdeII GATC 4 cut(s) 257, 358, 448, 691
NlaIII CATG 3 cut(s) 125, 275, 698
NlaIV GGNNCC 1 cut(s) 401
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 324
NspV TTCGAA 1 cut(s) 338
PagI TCATGA 1 cut(s) 694
PctI GAATGC 1 cut(s) 7
PfeI GAWTC 1 cut(s) 335
PflMI CCANNNNNTGG 2 cut(s) 245, 743
PkrI GCNGC 2 cut(s) 16, 37
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
PspN4I GGNNCC 1 cut(s) 401
PspPI GGNCC 1 cut(s) 399
PsuI RGATCY 1 cut(s) 448
SalI GTCGAC 1 cut(s) 651
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 15, 36
Sau3AI GATC 4 cut(s) 257, 358, 448, 691
Sau96I GGNCC 1 cut(s) 399
SchI GAGTC 1 cut(s) 647
SduI GDGCHC 1 cut(s) 84
SfaNI GCATC 2 cut(s) 201, 331
SfuI TTCGAA 1 cut(s) 338
SpeI ACTAGT 1 cut(s) 176
Sse9I AATT 3 cut(s) 39, 222, 300
SsiI CCGC 2 cut(s) 15, 201
SspMI CTAG 2 cut(s) 177, 263
TaaI ACNGT 3 cut(s) 243, 254, 375
TaqI TCGA 3 cut(s) 338, 418, 652
TasI AATT 3 cut(s) 39, 222, 300
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 343
Van91I CCANNNNNTGG 2 cut(s) 245, 743
XmiI GTMKAC 1 cut(s) 652
XspI CTAG 2 cut(s) 177, 263
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.