Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005157

Physical Location & Seq

Genomic Coordinates Forward (+)

21970456 .. 21972066

Gene Structure 1611 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1106800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGGCGACTTCTTCGTCTTCTCCCTCCCACCAAGATGACCCACAAAAGGTCCAATTCCCGTTGGACTCCGCCGCGTACGAAAACCTCCAAGAAATCGGCTCTGGCGACAAGGTCTTCGTCTACAAGGCCGTCTGCGTCCCCATGAACTCTGCAATCGTCGCAATCAAGTCCGTCAACCTCAATCAATCCGCTGCCCATTTCGAGAGTAAGCTGCGCGACACCAAGACTCTGTCTCTTTCCCACCCCAACATTCTCGGCGATCATTGCTCCTTCACCACCGCCGAGCGCCGTCTCTGGGTGGTCATGCCGTTCATGACCTTCGGCTCTCTGCAATCCGTAATAATCGCCCCTTCAGCTTTCCCGGAAGGATTACCAGAGCCCTGCATTGCAGTCGTCCTGAAGGAGACGCTAAACGCCCTGTCGTATCTCCATGGCCACGGACGCTTCCACGGCGACATCAAGCCCGGAAATATCTTGGTTGACTCTGATGGGTCCGTTAAGATCGCAGATTTCGGGGTTTCTTCTTCTTTCTACGACGCCGATTTTTCAACTGGGGCAAACGACTCTACAAGGCCGTATTGGGTGGCGCCCGGCGGAGGTGGATGCAAGGCGGATATCTGGGCATTTGGCATCACGGCGCTGGAATTGGCTCTGGGCGGCCCTCCCCTGTCTGGCTTGCCGCTGTTATTGGGGACTCAAATTATGAAGGTCAAGAACAGGTTCCGGCTTGCGGATTACGAACACATCATCAAGGATTTCCGGAACCAGAAGTACTCTGCGGCTTTCAAAGACTTGGTAGGTTCTTGCCTTGATCGGGACCCGGAGAAGAGGCCGGCGGCAGAGGCGCTGCTGAAGCACCCGTTTTTCCACGACTGCGGGGGCAAGGACTATCTGGTGGGGACTGTGCTTCCGCATTTGGAGAGCGTCGAGGAGAGGTTCAAGAAAAGAAGCAGGGGTTTGGCTCACGTGTTGGGCAAGGTAGACTATGAAGAAGTGAGGGGTGAGGATATGGGGTTTAAAGGATGGAATTTCAATGTGGACGACTTTGTTTTCAACCCAGTTTTTCCGATTGCGTCGTCTTCTACTCCCAGCTCTGAGTCTTCCGACGGCATCGAATTCCGGCTATCAGCATGTGTAAGGAGGTTGGCAAAGCAATTACAGATTGTGATGATGACTACTGGTGAGTTGAAGGGAGCAATTGAGGCGAGCATTGAGGAACCCGTAGAGATTTGGATGATGAAGAATGCGTTGGAGAAAGAGAAGGAAGATATTATGGACCTGCAGATTAAATTGAGGGAGTTTGCTTTGAACCCGATTTATCCGGCTGGGTTGTATACTACTGTGTATTCTACTTCCAGCTTTCCGGCAGATGGCGGCAACGCTCGGCTGGTGTGGTTGCTAGTGGAATCGCTGGTGAAATTGGTGAGAATTGCAGGAAAATTGGAAATACAGATTAAGAATTTGATCAGCATTATTGATGAAGAATTGGGAGGAGGAGGGGAGGTTGAGGCGCTGAAGAGGAAGTTGGAGTCTGAGCACCAAACATGTTTGGGGTTTCAGGTAGCGTTGGAGCTGATCAAGTTAAGTTTGGACCCTAATGAAAGAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010119 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1902456

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

537

Amino Acids

58.93

Weight (kDa)

5.5

Isoelectric Point (pI)

41.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	26 - 289	8e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	29 - 285	7.1e-27	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14480
fragaria_vesca	FvH4_4g03000
malus_domestica	MD01G1106400.v1.1 MD01G1106800.v1.1 MD02G1095900.v1.1 MD04G1063400.v1.1 MD04G1063500.v1.1 MD07G1020600.v1.1 MD07G1020800.v1.1 MD16G1212100.v1.1
prunus_persica	Prupe.1G031800_v2.0.a1 Prupe.1G033800_v2.0.a1 Prupe.1G034000_v2.0.a1 Prupe.4G193100_v2.0.a1
pyrus_communis	pycom01g13320 pycom01g13330 pycom01g13350 pycom07g01600 pycom16g17810
rosa_chinensis	RchiOBHm_Chr4g0391181
rosa_laevigata	RLG00000009879
rosa_multiflora	Rmu_co8201760.1_g000001 Rmu_sc0000040.1_g000009
rosa_roxburghii	Rroxscaffold_5G00336980
rosa_rugosa	Rorug03G0334900
rosa_samantha	Rh4AG035700 Rh4BG030700 Rh4CG039000 Rh4DG033200
rosa_wichuraiana	Rw4G002800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	13
Acc36I	ACCTGC	1 cut(s)	1287
AccB1I	GGYRCC	1 cut(s)	586
AccI	GTMKAC	3 cut(s)	120, 981, 1334
AccII	CGCG	2 cut(s)	74, 216
AccIII	TCCGGA	1 cut(s)	759
AcoI	YGGCCR	1 cut(s)	433
AcsI	RAATTY	3 cut(s)	1027, 1115, 1459
AcuI	CTGAAG	4 cut(s)	336, 419, 872, 1535
AcvI	CACGTG	1 cut(s)	967
AcyI	GRCGYC	2 cut(s)	537, 587
AfaI	GTAC	2 cut(s)	77, 773
AfiI	CCNNNNNNNGG	7 cut(s)	46, 295, 596, 671, 730, 814, 1370
AflIII	ACRYGT	2 cut(s)	966, 1544
AgsI	TTSAA	7 cut(s)	549, 787, 940, 1033, 1054, 1189, 1309
AjuI	GAANNNNNNNTTGG	4 cut(s)	1232, 1264, 1508, 1540
AluBI	AGCT	5 cut(s)	211, 356, 1092, 1359, 1573
AluI	AGCT	5 cut(s)	211, 356, 1092, 1359, 1573
Alw21I	GWGCWC	1 cut(s)	1539
Alw26I	GTCTC	3 cut(s)	237, 296, 398
Aor13HI	TCCGGA	1 cut(s)	759
AoxI	GGCC	5 cut(s)	126, 433, 572, 658, 830
ApeKI	GCWGC	3 cut(s)	191, 211, 847
ApoI	RAATTY	3 cut(s)	1027, 1115, 1459
ArsI	GACNNNNNNTTYG	2 cut(s)	98, 130
Asp700I	GAANNNNTTC	1 cut(s)	719
AspLEI	GCGC	6 cut(s)	216, 288, 589, 640, 847, 1513
AspS9I	GGNCC	6 cut(s)	49, 492, 659, 817, 1276, 1591
AsuC2I	CCSGG	4 cut(s)	362, 465, 591, 821
AsuHPI	GGTGA	5 cut(s)	265, 1013, 1193, 1426, 1435
AvaII	GGWCC	5 cut(s)	49, 492, 817, 1276, 1591
BalI	TGGCCA	1 cut(s)	435
BanI	GGYRCC	1 cut(s)	586
BanII	GRGCYC	1 cut(s)	381
BbrPI	CACGTG	1 cut(s)	967
BbsI	GAAGAC	4 cut(s)	9, 106, 1071, 1092
Bbv12I	GWGCWC	1 cut(s)	1539
BbvI	GCAGC	3 cut(s)	178, 198, 834
BccI	CCATC	3 cut(s)	482, 1017, 1364
BceAI	ACGGC	7 cut(s)	113, 273, 292, 466, 559, 651, 1123
BcgI	CGANNNNNNTGC	2 cut(s)	373, 407
BclI	TGATCA	2 cut(s)	1464, 1575
BcnI	CCSGG	4 cut(s)	362, 465, 591, 821
BcoDI	GTCTC	3 cut(s)	237, 296, 398
BfaI	CTAG	1 cut(s)	1400
BfmI	CTRYAG	1 cut(s)	1280
BfoI	RGCGCY	5 cut(s)	289, 590, 641, 848, 1514
BfuAI	ACCTGC	1 cut(s)	1287
BisI	GCNGC	9 cut(s)	72, 192, 212, 658, 680, 780, 837, 848, 1375
BlsI	GCNGC	9 cut(s)	73, 193, 213, 659, 681, 781, 838, 849, 1376
BmcAI	AGTACT	1 cut(s)	773
Bme1390I	CCNGG	4 cut(s)	362, 465, 591, 821
Bme18I	GGWCC	5 cut(s)	49, 492, 817, 1276, 1591
BmgT120I	GGNCC	6 cut(s)	49, 492, 659, 817, 1276, 1591
BmiI	GGNNCC	8 cut(s)	493, 588, 722, 764, 818, 819, 1218, 1593
BmrFI	CCNGG	4 cut(s)	362, 465, 591, 821
BmrI	ACTGGG	2 cut(s)	561, 1052
BmsI	GCATC	3 cut(s)	593, 639, 1119
BmuI	ACTGGG	2 cut(s)	561, 1052
BpiI	GAAGAC	4 cut(s)	9, 106, 1071, 1092
BpuMI	CCSGG	4 cut(s)	362, 465, 591, 821
BsaAI	YACGTR	1 cut(s)	967
BsaHI	GRCGYC	2 cut(s)	537, 587
BsaJI	CCNNGG	3 cut(s)	430, 436, 448
BsaWI	WCCGGW	1 cut(s)	759
BsaXI	ACNNNNNCTCC	2 cut(s)	1488, 1518
Bsc4I	CCNNNNNNNGG	7 cut(s)	46, 295, 596, 671, 730, 814, 1370
Bse118I	RCCGGY	1 cut(s)	832
Bse1I	ACTGG	3 cut(s)	556, 1058, 1183
Bse3DI	GCAATG	2 cut(s)	262, 384
BseAI	TCCGGA	1 cut(s)	759
BseDI	CCNNGG	3 cut(s)	430, 436, 448
BseGI	GGATG	3 cut(s)	608, 1028, 1239
BseLI	CCNNNNNNNGG	7 cut(s)	46, 295, 596, 671, 730, 814, 1370
BseMI	GCAATG	2 cut(s)	262, 384
BseMII	CTCAG	2 cut(s)	1086, 1524
BseNI	ACTGG	3 cut(s)	556, 1058, 1183
BseRI	GAGGAG	3 cut(s)	944, 1506, 1509
BseXI	GCAGC	3 cut(s)	178, 198, 834
BseYI	CCCAGC	2 cut(s)	1088, 1325
Bsh1236I	CGCG	2 cut(s)	74, 216
BshFI	GGCC	5 cut(s)	128, 435, 574, 660, 832
BshNI	GGYRCC	1 cut(s)	586
BsiHKAI	GWGCWC	1 cut(s)	1539
BsiWI	CGTACG	1 cut(s)	75
BslFI	GGGAC	4 cut(s)	122, 706, 830, 913
BslI	CCNNNNNNNGG	7 cut(s)	46, 295, 596, 671, 730, 814, 1370
BsmAI	GTCTC	3 cut(s)	237, 296, 398
BsmBI	CGTCTC	2 cut(s)	296, 398
BsmFI	GGGAC	4 cut(s)	122, 706, 830, 913
BsmI	GAATGC	1 cut(s)	1249
BsnI	GGCC	5 cut(s)	128, 435, 574, 660, 832
Bsp1286I	GDGCHC	2 cut(s)	381, 1539
Bsp13I	TCCGGA	1 cut(s)	759
Bsp143I	GATC	5 cut(s)	259, 501, 811, 1464, 1575
Bsp19I	CCATGG	1 cut(s)	430
BspANI	GGCC	5 cut(s)	128, 435, 574, 660, 832
BspCNI	CTCAG	2 cut(s)	1087, 1525
BspEI	TCCGGA	1 cut(s)	759
BspFNI	CGCG	2 cut(s)	74, 216
BspHI	TCATGA	1 cut(s)	312
BspLI	GGNNCC	8 cut(s)	493, 588, 722, 764, 818, 819, 1218, 1593
BspMAI	CTGCAG	1 cut(s)	1284
BspMI	ACCTGC	1 cut(s)	1287
BspT107I	GGYRCC	1 cut(s)	586
BsrDI	GCAATG	2 cut(s)	262, 384
BsrFI	RCCGGY	1 cut(s)	832
BsrI	ACTGG	3 cut(s)	556, 1058, 1183
BssAI	RCCGGY	1 cut(s)	832
BssECI	CCNNGG	3 cut(s)	430, 436, 448
BssMI	GATC	5 cut(s)	259, 501, 811, 1464, 1575
BssNAI	GTATAC	1 cut(s)	1335
BssNI	GRCGYC	2 cut(s)	537, 587
BssT1I	CCWWGG	1 cut(s)	430
Bst1107I	GTATAC	1 cut(s)	1335
Bst4CI	ACNGT	2 cut(s)	904, 1342
Bst6I	CTCTTC	2 cut(s)	821, 1511
BstACI	GRCGYC	2 cut(s)	537, 587
BstBAI	YACGTR	1 cut(s)	967
BstC8I	GCNNGC	4 cut(s)	677, 729, 834, 1207
BstDEI	CTNAG	2 cut(s)	1095, 1533
BstDSI	CCRYGG	3 cut(s)	430, 436, 448
BstF5I	GGATG	3 cut(s)	608, 1028, 1239
BstFNI	CGCG	2 cut(s)	74, 216
BstH2I	RGCGCY	5 cut(s)	289, 590, 641, 848, 1514
BstHHI	GCGC	6 cut(s)	216, 288, 589, 640, 847, 1513
BstKTI	GATC	5 cut(s)	262, 504, 814, 1467, 1578
BstMAI	GTCTC	3 cut(s)	237, 296, 398
BstMBI	GATC	5 cut(s)	259, 501, 811, 1464, 1575
BstMWI	GCNNNNNNNGC	8 cut(s)	158, 264, 353, 441, 450, 842, 853, 1202
BstNSI	RCATGY	2 cut(s)	1134, 1548
BstSCI	CCNGG	4 cut(s)	360, 463, 589, 819
BstSFI	CTRYAG	1 cut(s)	1280
BstUI	CGCG	2 cut(s)	74, 216
BstV1I	GCAGC	3 cut(s)	178, 198, 834
BstV2I	GAAGAC	4 cut(s)	9, 106, 1071, 1092
BstZ17I	GTATAC	1 cut(s)	1335
BsuRI	GGCC	5 cut(s)	128, 435, 574, 660, 832
BtgI	CCRYGG	3 cut(s)	430, 436, 448
BtsCI	GGATG	3 cut(s)	608, 1028, 1239
BveI	ACCTGC	1 cut(s)	1287
Cac8I	GCNNGC	4 cut(s)	677, 729, 834, 1207
CciI	TCATGA	1 cut(s)	312
CfoI	GCGC	6 cut(s)	216, 288, 589, 640, 847, 1513
Cfr10I	RCCGGY	1 cut(s)	832
Cfr13I	GGNCC	6 cut(s)	49, 492, 659, 817, 1276, 1591
CseI	GACGC	6 cut(s)	124, 415, 450, 545, 913, 1062
Csp6I	GTAC	2 cut(s)	76, 772
CviAII	CATG	6 cut(s)	142, 304, 313, 431, 1131, 1545
CviQI	GTAC	2 cut(s)	76, 772
DdeI	CTNAG	2 cut(s)	1095, 1533
DinI	GGCGCC	1 cut(s)	588
DpnI	GATC	5 cut(s)	261, 503, 813, 1466, 1577
DpnII	GATC	5 cut(s)	259, 501, 811, 1464, 1575
DraI	TTTAAA	1 cut(s)	1018
DrdI	GACNNNNNNGTC	1 cut(s)	13
DseDI	GACNNNNNNGTC	1 cut(s)	13
EaeI	YGGCCR	1 cut(s)	433
Eam1104I	CTCTTC	2 cut(s)	821, 1511
EarI	CTCTTC	2 cut(s)	821, 1511
EciI	GGCGGA	3 cut(s)	58, 609, 626
Eco130I	CCWWGG	1 cut(s)	430
Eco24I	GRGCYC	1 cut(s)	381
Eco32I	GATATC	1 cut(s)	616
Eco47I	GGWCC	5 cut(s)	49, 492, 817, 1276, 1591
Eco57I	CTGAAG	4 cut(s)	336, 419, 872, 1535
Eco72I	CACGTG	1 cut(s)	967
EcoO109I	RGGNCCY	1 cut(s)	817
EcoRI	GAATTC	1 cut(s)	1115
EcoRV	GATATC	1 cut(s)	616
EcoT14I	CCWWGG	1 cut(s)	430
EcoT38I	GRGCYC	1 cut(s)	381
EgeI	GGCGCC	1 cut(s)	588
EheI	GGCGCC	1 cut(s)	588
ErhI	CCWWGG	1 cut(s)	430
Esp3I	CGTCTC	2 cut(s)	296, 398
FaeI	CATG	6 cut(s)	145, 307, 316, 434, 1134, 1548
FaqI	GGGAC	4 cut(s)	122, 706, 830, 913
FatI	CATG	6 cut(s)	141, 303, 312, 430, 1130, 1544
FauI	CCCGC	1 cut(s)	869
FbaI	TGATCA	2 cut(s)	1464, 1575
FblI	GTMKAC	3 cut(s)	120, 981, 1334
Fnu4HI	GCNGC	9 cut(s)	72, 192, 212, 658, 680, 780, 837, 848, 1375
FokI	GGATG	3 cut(s)	615, 1035, 1246
FriOI	GRGCYC	1 cut(s)	381
Fsp4HI	GCNGC	9 cut(s)	72, 192, 212, 658, 680, 780, 837, 848, 1375
FspBI	CTAG	1 cut(s)	1400
GlaI	GCGC	6 cut(s)	215, 287, 588, 639, 846, 1512
GluI	GCNGC	9 cut(s)	72, 192, 212, 658, 680, 780, 837, 848, 1375
GsaI	CCCAGC	2 cut(s)	1092, 1329
HaeII	RGCGCY	5 cut(s)	289, 590, 641, 848, 1514
HaeIII	GGCC	5 cut(s)	128, 435, 574, 660, 832
HgaI	GACGC	6 cut(s)	124, 415, 450, 545, 913, 1062
HhaI	GCGC	6 cut(s)	216, 288, 589, 640, 847, 1513
Hin1I	GRCGYC	2 cut(s)	537, 587
Hin1II	CATG	6 cut(s)	145, 307, 316, 434, 1134, 1548
Hin6I	GCGC	6 cut(s)	214, 286, 587, 638, 845, 1511
HinP1I	GCGC	6 cut(s)	214, 286, 587, 638, 845, 1511
HincII	GTYRAC	2 cut(s)	175, 481
HindII	GTYRAC	2 cut(s)	175, 481
HinfI	GANTC	8 cut(s)	65, 226, 482, 563, 694, 1097, 1406, 1529
HphI	GGTGA	5 cut(s)	265, 1013, 1193, 1426, 1435
Hpy166II	GTNNAC	6 cut(s)	121, 175, 481, 982, 1039, 1335
Hpy188I	TCNGA	5 cut(s)	487, 1068, 1096, 1105, 1534
Hpy188III	TCNNGA	7 cut(s)	202, 313, 397, 712, 760, 815, 940
Hpy8I	GTNNAC	6 cut(s)	121, 175, 481, 982, 1039, 1335
Hpy99I	CGWCG	5 cut(s)	161, 539, 929, 1078, 1109
HpyAV	CCTTC	8 cut(s)	280, 328, 359, 360, 394, 700, 1183, 1255
HpyCH4III	ACNGT	2 cut(s)	904, 1342
HpyCH4IV	ACGT	1 cut(s)	966
HpyCH4V	TGCA	7 cut(s)	152, 331, 384, 389, 606, 1282, 1433
HpyF10VI	GCNNNNNNNGC	8 cut(s)	158, 264, 353, 441, 450, 842, 853, 1202
HpyF3I	CTNAG	2 cut(s)	1095, 1533
HpySE526I	ACGT	1 cut(s)	966
Hsp92I	GRCGYC	2 cut(s)	537, 587
Hsp92II	CATG	6 cut(s)	145, 307, 316, 434, 1134, 1548
HspAI	GCGC	6 cut(s)	214, 286, 587, 638, 845, 1511
KasI	GGCGCC	1 cut(s)	586
KflI	GGGWCCC	1 cut(s)	817
Kpn2I	TCCGGA	1 cut(s)	759
KroI	GCCGGC	1 cut(s)	832
KroNI	GCCGGC	1 cut(s)	834
Ksp22I	TGATCA	2 cut(s)	1464, 1575
Kzo9I	GATC	5 cut(s)	259, 501, 811, 1464, 1575
LmnI	GCTCC	3 cut(s)	272, 1193, 1570
Lsp1109I	GCAGC	3 cut(s)	178, 198, 834
LweI	GCATC	3 cut(s)	593, 639, 1119
MaeI	CTAG	1 cut(s)	1400
MaeII	ACGT	1 cut(s)	966
MalI	GATC	5 cut(s)	261, 503, 813, 1466, 1577
MboI	GATC	5 cut(s)	259, 501, 811, 1464, 1575
MfeI	CAATTG	1 cut(s)	1197
MhlI	GDGCHC	2 cut(s)	381, 1539
MlsI	TGGCCA	1 cut(s)	435
MluNI	TGGCCA	1 cut(s)	435
Mly113I	GGCGCC	1 cut(s)	587
MlyI	GAGTC	7 cut(s)	59, 220, 476, 557, 688, 1106, 1538
MmeI	TCCRAC	5 cut(s)	42, 1128, 1230, 1506, 1548
Mox20I	TGGCCA	1 cut(s)	435
MroI	TCCGGA	1 cut(s)	759
MroNI	GCCGGC	1 cut(s)	832
MroXI	GAANNNNTTC	1 cut(s)	719
MscI	TGGCCA	1 cut(s)	435
MseI	TTAA	5 cut(s)	498, 1017, 1287, 1455, 1583
MslI	CAYNNNNRTG	1 cut(s)	33
Msp20I	TGGCCA	1 cut(s)	435
MspA1I	CMGCKG	2 cut(s)	191, 682
MspR9I	CCNGG	4 cut(s)	362, 465, 591, 821
MunI	CAATTG	1 cut(s)	1197
Mva1269I	GAATGC	1 cut(s)	1249
MvnI	CGCG	2 cut(s)	74, 216
MwoI	GCNNNNNNNGC	8 cut(s)	158, 264, 353, 441, 450, 842, 853, 1202
NaeI	GCCGGC	1 cut(s)	834
NarI	GGCGCC	1 cut(s)	587
NciI	CCSGG	4 cut(s)	362, 465, 591, 821
NcoI	CCATGG	1 cut(s)	430
NdeII	GATC	5 cut(s)	259, 501, 811, 1464, 1575
NgoMIV	GCCGGC	1 cut(s)	832
NlaIII	CATG	6 cut(s)	145, 307, 316, 434, 1134, 1548
NlaIV	GGNNCC	8 cut(s)	493, 588, 722, 764, 818, 819, 1218, 1593
NmeAIII	GCCGAG	3 cut(s)	234, 307, 1363
NspI	RCATGY	2 cut(s)	1134, 1548
PagI	TCATGA	1 cut(s)	312
PciI	ACATGT	1 cut(s)	1544
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PctI	GAATGC	1 cut(s)	1249
PdiI	GCCGGC	1 cut(s)	834
PdmI	GAANNNNTTC	1 cut(s)	719
PfeI	GAWTC	1 cut(s)	1406
Pfl23II	CGTACG	1 cut(s)	75
PflFI	GACNNNGTC	2 cut(s)	110, 229
PfoI	TCCNGGA	1 cut(s)	360
PkrI	GCNGC	9 cut(s)	73, 193, 213, 659, 681, 781, 838, 849, 1376
PleI	GAGTC	7 cut(s)	59, 220, 476, 557, 688, 1105, 1537
PluTI	GGCGCC	1 cut(s)	590
PmaCI	CACGTG	1 cut(s)	967
PmlI	CACGTG	1 cut(s)	967
PpsI	GAGTC	7 cut(s)	59, 220, 476, 557, 688, 1105, 1537
Ppu21I	YACGTR	1 cut(s)	967
PpuMI	RGGWCCY	1 cut(s)	817
PscI	ACATGT	1 cut(s)	1544
Psp5II	RGGWCCY	1 cut(s)	817
PspCI	CACGTG	1 cut(s)	967
PspFI	CCCAGC	2 cut(s)	1088, 1325
PspLI	CGTACG	1 cut(s)	75
PspN4I	GGNNCC	8 cut(s)	493, 588, 722, 764, 818, 819, 1218, 1593
PspPI	GGNCC	6 cut(s)	49, 492, 659, 817, 1276, 1591
PspPPI	RGGWCCY	1 cut(s)	817
PsrI	GAACNNNNNNTAC	2 cut(s)	755, 787
PstI	CTGCAG	1 cut(s)	1284
PsyI	GACNNNGTC	2 cut(s)	110, 229
RsaI	GTAC	2 cut(s)	77, 773
RsaNI	GTAC	2 cut(s)	76, 772
RseI	CAYNNNNRTG	1 cut(s)	33
SaqAI	TTAA	5 cut(s)	498, 1017, 1287, 1455, 1583
SatI	GCNGC	9 cut(s)	72, 192, 212, 658, 680, 780, 837, 848, 1375
Sau3AI	GATC	5 cut(s)	259, 501, 811, 1464, 1575
Sau96I	GGNCC	6 cut(s)	49, 492, 659, 817, 1276, 1591
ScaI	AGTACT	1 cut(s)	773
SchI	GAGTC	7 cut(s)	59, 220, 476, 557, 688, 1106, 1538
ScrFI	CCNGG	4 cut(s)	362, 465, 591, 821
SduI	GDGCHC	2 cut(s)	381, 1539
SfaNI	GCATC	3 cut(s)	593, 639, 1119
SfcI	CTRYAG	1 cut(s)	1280
SfoI	GGCGCC	1 cut(s)	588
SinI	GGWCC	5 cut(s)	49, 492, 817, 1276, 1591
SmiMI	CAYNNNNRTG	1 cut(s)	33
SspDI	GGCGCC	1 cut(s)	586
SspMI	CTAG	1 cut(s)	1400
StyD4I	CCNGG	4 cut(s)	360, 463, 589, 819
StyI	CCWWGG	1 cut(s)	430
TaaI	ACNGT	2 cut(s)	904, 1342
TaiI	ACGT	1 cut(s)	969
TaqI	TCGA	3 cut(s)	201, 927, 1113
TatI	WGTACW	1 cut(s)	771
TauI	GCSGC	6 cut(s)	74, 660, 682, 782, 839, 1377
TfiI	GAWTC	1 cut(s)	1406
Tru1I	TTAA	5 cut(s)	498, 1017, 1287, 1455, 1583
Tru9I	TTAA	5 cut(s)	498, 1017, 1287, 1455, 1583
TseI	GCWGC	3 cut(s)	191, 211, 847
TspDTI	ATGAA	6 cut(s)	158, 301, 719, 1002, 1253, 1494
TspGWI	ACGGA	4 cut(s)	160, 325, 453, 484
Tth111I	GACNNNGTC	2 cut(s)	110, 229
VpaK11BI	GGWCC	5 cut(s)	49, 492, 817, 1276, 1591
XapI	RAATTY	3 cut(s)	1027, 1115, 1459
XceI	RCATGY	2 cut(s)	1134, 1548
XmiI	GTMKAC	3 cut(s)	120, 981, 1334
XmnI	GAANNNNTTC	1 cut(s)	719
XspI	CTAG	1 cut(s)	1400
ZrmI	AGTACT	1 cut(s)	773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.