Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005157

Physical Location & Seq

Genomic Coordinates Reverse (-)

8386924 .. 8388495

Gene Structure 1572 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1063500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGGCGACTTCTTCGACTTCTTCCTCCCACCAAGATAACCCACAAAAGGTCCAATTCCCGTTGGACTCCGCCGCGTACAAAGACCTCCAAGAAATCGGCTCTGGCGACAAGGCCGTCGTCGTCTACAAGGCCGTCTGCGTCCCCATGAACTCTGCAATCGTCGCAATCAAGTCCGTTGACCTCAATCAATCCACGGCCGATTTCGAAAGCAAGCTACGCGATACCAAGACTCTGTCTCTTTCCCACCACAACATTCTCAGCGACCATTGCTCCTTCACCACCGCCGACCGCCGTCTCTGGGTGGTTATGCCGTTCATGGCCTTCGGCTCTCTAATCTCCTCCTCTGCTTTCCCGGAAGGATTACCAGAGCCCTGCGTTGCTGCTGTCCTGGAAGAGACGCTAAACGCCATGTCCTATCTCCATAGTCAGGAACAAGTCCACGGCGACATCAAACCCGGAAACATCTTGGTTGACTCTGATGGGTCTGTTAAGCTCGCCAATTTCGGGGTCTCTGCTTCTTTCTACGGCGCCGATCATTCAACTGGGACAAACGACACTTCTGCAACGCCGTATTGGGTGGCGCCCAACGGAGGTGGATGCAAGGCGGATATCTGGGCATTTGGGATCACGGCTCTGGAATTGGCTCTCGGCGGCCCTCCCGTGTCTGACCTGCCGCCGTTTTTAGAGTCTCAGATTCTGAAGGTCAAGAACAGGTTCCCGTTTTCCGATTACAAAAACATCATCAAAGATTTCAAGAACAAGAAGTACTCAGCGGATTTCAAGGACATGGTGCGTTCTTGCCTTGATCTGGACCCGGAAAAGAGGCCGGCGGCGGAGACGCTGCTGAAGCACTCGTTTTTCCACAACTACAGTGGCAAGGATTTTCTGGTGGGGACTGTGCTTCCGCATTTGGAGAGCGTTGAGGAGAGGTTCAAGAAAAGAAACAGGGGTTTGGGTGACTTGTTGGGCAAGGAAAAATATGAACTAAGGGCTGAGAATATGGGGTTTAGCGGATGGAATTTCAATGAGGACGACTTTGTTTTCAACCCGGTTTTTTCCACTGAGTCGTCTTCTACTTCCAGCTCTGAGTCTTCTGGCGGCGGCAAATCCCCGCTGTCAATATTTGCAACGAGGTTGGCAAAGCATTTAGAGATTGTGGTGATGATTATTGATCTGTTGAAGGGAGCAGTTGAGGTGAGCACACAGGATCAAGTAGAGATTCAGAGGCTGAAGGGTGAGTTGGAGATCAAGAAGGAAGATATTTTGCAGTTCCAGGATAACTTGGAGAGGTTTGTTTTGGACCTGGCTATTCCAGCTGCGTCGTCTTCTACTTCCAGCCTTCCGGCTGAGTCTTCCAACCGCGGCAATGCACGGCTGGTGGAATTGCAGGAAATTGCAAGAAATTTGGACCAGCAGATGGAGAATGTGCAGAGCATTATTGGTCAGTTGGGACAATCAGAAGAGGTTCAGCTGCTGAAGAGTGAGTTGGAGTCTGAGTACGACAGATGTGTGAACTTTCAGGTAGAGTTGGAGGTGATCATGCAAAAGTTGTACACTGATGAAAGTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010119 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1902456

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

524

Amino Acids

57.67

Weight (kDa)

4.94

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	26 - 287	9.4e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	30 - 283	1.4e-26	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14480
fragaria_vesca	FvH4_4g03000
malus_domestica	MD01G1106400.v1.1 MD01G1106800.v1.1 MD02G1095900.v1.1 MD04G1063400.v1.1 MD04G1063500.v1.1 MD07G1020600.v1.1 MD07G1020800.v1.1 MD16G1212100.v1.1
prunus_persica	Prupe.1G031800_v2.0.a1 Prupe.1G033800_v2.0.a1 Prupe.1G034000_v2.0.a1 Prupe.4G193100_v2.0.a1
pyrus_communis	pycom01g13320 pycom01g13330 pycom01g13350 pycom07g01600 pycom16g17810
rosa_chinensis	RchiOBHm_Chr4g0391181
rosa_laevigata	RLG00000009879
rosa_multiflora	Rmu_co8201760.1_g000001 Rmu_sc0000040.1_g000009
rosa_roxburghii	Rroxscaffold_5G00336980
rosa_rugosa	Rorug03G0334900
rosa_samantha	Rh4AG035700 Rh4BG030700 Rh4CG039000 Rh4DG033200
rosa_wichuraiana	Rw4G002800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	113
Acc36I	ACCTGC	1 cut(s)	678
AccB1I	GGYRCC	2 cut(s)	527, 580
AccB7I	CCANNNNNTGG	1 cut(s)	1417
AccI	GTMKAC	1 cut(s)	123
AccII	CGCG	3 cut(s)	74, 219, 1362
AclWI	GGATC	2 cut(s)	632, 1215
AcoI	YGGCCR	1 cut(s)	195
AcsI	RAATTY	2 cut(s)	1018, 1402
AcuI	CTGAAG	4 cut(s)	719, 866, 1250, 1496
AcyI	GRCGYC	2 cut(s)	528, 581
AfaI	GTAC	4 cut(s)	77, 767, 1499, 1553
AfiI	CCNNNNNNNGG	7 cut(s)	46, 298, 427, 504, 590, 808, 1417
AgsI	TTSAA	7 cut(s)	540, 754, 781, 934, 1024, 1045, 1180
AjnI	CCWGG	3 cut(s)	387, 1272, 1302
AjuI	GAANNNNNNNTTGG	4 cut(s)	1223, 1255, 1469, 1501
AluBI	AGCT	5 cut(s)	214, 493, 1083, 1316, 1471
AluI	AGCT	5 cut(s)	214, 493, 1083, 1316, 1471
Alw21I	GWGCWC	1 cut(s)	1202
Alw26I	GTCTC	6 cut(s)	240, 299, 389, 514, 693, 830
AlwI	GGATC	2 cut(s)	632, 1215
AlwNI	CAGNNNCTG	3 cut(s)	697, 1228, 1474
AoxI	GGCC	6 cut(s)	111, 129, 195, 318, 652, 824
ApeKI	GCWGC	4 cut(s)	380, 841, 1316, 1471
ApoI	RAATTY	2 cut(s)	1018, 1402
Asp700I	GAANNNNTTC	2 cut(s)	713, 1464
AspLEI	GCGC	2 cut(s)	530, 583
AspS9I	GGNCC	5 cut(s)	49, 653, 811, 1300, 1408
AsuC2I	CCSGG	4 cut(s)	353, 456, 815, 1049
AsuHPI	GGTGA	6 cut(s)	268, 968, 1171, 1207, 1247, 1546
AsuII	TTCGAA	1 cut(s)	204
AvaII	GGWCC	4 cut(s)	49, 811, 1300, 1408
BanI	GGYRCC	2 cut(s)	527, 580
BanII	GRGCYC	1 cut(s)	372
BbsI	GAAGAC	4 cut(s)	1062, 1083, 1317, 1344
Bbv12I	GWGCWC	1 cut(s)	1202
BbvI	GCAGC	4 cut(s)	367, 828, 1303, 1458
BccI	CCATC	3 cut(s)	473, 1008, 1411
BcgI	CGANNNNNNTGC	2 cut(s)	542, 576
BciT130I	CCWGG	3 cut(s)	389, 1274, 1304
BclI	TGATCA	1 cut(s)	1536
BcnI	CCSGG	4 cut(s)	353, 456, 815, 1049
BcoDI	GTCTC	6 cut(s)	240, 299, 389, 514, 693, 830
BfmI	CTRYAG	1 cut(s)	868
BfoI	RGCGCY	2 cut(s)	531, 584
BfuAI	ACCTGC	1 cut(s)	678
BmcAI	AGTACT	1 cut(s)	767
Bme1390I	CCNGG	7 cut(s)	353, 389, 456, 815, 1049, 1274, 1304
Bme18I	GGWCC	4 cut(s)	49, 811, 1300, 1408
BmgT120I	GGNCC	5 cut(s)	49, 653, 811, 1300, 1408
BmiI	GGNNCC	4 cut(s)	529, 582, 716, 813
BmrFI	CCNGG	7 cut(s)	353, 389, 456, 815, 1049, 1274, 1304
BmrI	ACTGGG	1 cut(s)	552
BmsI	GCATC	1 cut(s)	587
BmuI	ACTGGG	1 cut(s)	552
BoxI	GACNNNNGTC	1 cut(s)	291
BpiI	GAAGAC	4 cut(s)	1062, 1083, 1317, 1344
Bpu14I	TTCGAA	1 cut(s)	204
BpuMI	CCSGG	4 cut(s)	353, 456, 815, 1049
BsaHI	GRCGYC	2 cut(s)	528, 581
BsaI	GGTCTC	1 cut(s)	514
BsaJI	CCNNGG	3 cut(s)	192, 439, 1360
BsaXI	ACNNNNNCTCC	4 cut(s)	254, 284, 1481, 1511
Bsc4I	CCNNNNNNNGG	7 cut(s)	46, 298, 427, 504, 590, 808, 1417
Bse118I	RCCGGY	1 cut(s)	826
Bse1I	ACTGG	1 cut(s)	547
Bse3DI	GCAATG	2 cut(s)	265, 1372
BseBI	CCWGG	3 cut(s)	389, 1274, 1304
BseDI	CCNNGG	3 cut(s)	192, 439, 1360
BseGI	GGATG	2 cut(s)	602, 1019
BseLI	CCNNNNNNNGG	7 cut(s)	46, 298, 427, 504, 590, 808, 1417
BseMI	GCAATG	2 cut(s)	265, 1372
BseMII	CTCAG	8 cut(s)	271, 704, 783, 984, 1053, 1077, 1338, 1485
BseNI	ACTGG	1 cut(s)	547
BseRI	GAGGAG	3 cut(s)	328, 331, 938
BseX3I	CGGCCG	1 cut(s)	195
BseXI	GCAGC	4 cut(s)	367, 828, 1303, 1458
BsgI	GTGCAG	1 cut(s)	1448
Bsh1236I	CGCG	3 cut(s)	74, 219, 1362
Bsh1285I	CGRYCG	2 cut(s)	198, 289
BshFI	GGCC	6 cut(s)	113, 131, 197, 320, 654, 826
BshNI	GGYRCC	2 cut(s)	527, 580
BsiEI	CGRYCG	2 cut(s)	198, 289
BsiHKAI	GWGCWC	1 cut(s)	1202
BsiSI	CCGG	6 cut(s)	353, 456, 815, 827, 1049, 1343
BslFI	GGGAC	4 cut(s)	125, 559, 907, 1464
BslI	CCNNNNNNNGG	7 cut(s)	46, 298, 427, 504, 590, 808, 1417
BsmAI	GTCTC	6 cut(s)	240, 299, 389, 514, 693, 830
BsmBI	CGTCTC	3 cut(s)	299, 389, 830
BsmFI	GGGAC	4 cut(s)	125, 559, 907, 1464
BsnI	GGCC	6 cut(s)	113, 131, 197, 320, 654, 826
Bso31I	GGTCTC	1 cut(s)	514
Bsp119I	TTCGAA	1 cut(s)	204
Bsp1286I	GDGCHC	2 cut(s)	372, 1202
Bsp1407I	TGTACA	1 cut(s)	1551
Bsp143I	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
BspANI	GGCC	6 cut(s)	113, 131, 197, 320, 654, 826
BspCNI	CTCAG	8 cut(s)	270, 703, 782, 985, 1054, 1078, 1339, 1486
BspFNI	CGCG	3 cut(s)	74, 219, 1362
BspLI	GGNNCC	4 cut(s)	529, 582, 716, 813
BspMI	ACCTGC	1 cut(s)	678
BspPI	GGATC	2 cut(s)	632, 1215
BspT104I	TTCGAA	1 cut(s)	204
BspT107I	GGYRCC	2 cut(s)	527, 580
BspTNI	GGTCTC	1 cut(s)	514
BsrDI	GCAATG	2 cut(s)	265, 1372
BsrFI	RCCGGY	1 cut(s)	826
BsrGI	TGTACA	1 cut(s)	1551
BsrI	ACTGG	1 cut(s)	547
BssAI	RCCGGY	1 cut(s)	826
BssECI	CCNNGG	3 cut(s)	192, 439, 1360
BssMI	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
BssNI	GRCGYC	2 cut(s)	528, 581
Bst2UI	CCWGG	3 cut(s)	389, 1274, 1304
Bst4CI	ACNGT	2 cut(s)	872, 898
Bst6I	CTCTTC	3 cut(s)	387, 1455, 1472
BstACI	GRCGYC	2 cut(s)	528, 581
BstAUI	TGTACA	1 cut(s)	1551
BstBI	TTCGAA	1 cut(s)	204
BstC8I	GCNNGC	3 cut(s)	212, 495, 828
BstDEI	CTNAG	9 cut(s)	257, 690, 769, 986, 993, 1062, 1086, 1347, 1494
BstDSI	CCRYGG	3 cut(s)	192, 439, 1360
BstF5I	GGATG	2 cut(s)	602, 1019
BstFNI	CGCG	3 cut(s)	74, 219, 1362
BstH2I	RGCGCY	2 cut(s)	531, 584
BstHHI	GCGC	2 cut(s)	530, 583
BstKTI	GATC	7 cut(s)	535, 627, 808, 1174, 1210, 1248, 1539
BstMAI	GTCTC	6 cut(s)	240, 299, 389, 514, 693, 830
BstMBI	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
BstMCI	CGRYCG	2 cut(s)	198, 289
BstMWI	GCNNNNNNNGC	5 cut(s)	161, 216, 267, 847, 1313
BstNI	CCWGG	3 cut(s)	389, 1274, 1304
BstPAI	GACNNNNGTC	1 cut(s)	291
BstSCI	CCNGG	7 cut(s)	351, 387, 454, 813, 1047, 1272, 1302
BstSFI	CTRYAG	1 cut(s)	868
BstUI	CGCG	3 cut(s)	74, 219, 1362
BstV1I	GCAGC	4 cut(s)	367, 828, 1303, 1458
BstV2I	GAAGAC	4 cut(s)	1062, 1083, 1317, 1344
BstZI	CGGCCG	1 cut(s)	195
BsuRI	GGCC	6 cut(s)	113, 131, 197, 320, 654, 826
BtgI	CCRYGG	3 cut(s)	192, 439, 1360
BtsCI	GGATG	2 cut(s)	602, 1019
BtsIMutI	CAGTG	3 cut(s)	877, 1059, 1554
BveI	ACCTGC	1 cut(s)	678
Cac8I	GCNNGC	3 cut(s)	212, 495, 828
CaiI	CAGNNNCTG	3 cut(s)	697, 1228, 1474
CfoI	GCGC	2 cut(s)	530, 583
Cfr10I	RCCGGY	1 cut(s)	826
Cfr13I	GGNCC	5 cut(s)	49, 653, 811, 1300, 1408
Cfr42I	CCGCGG	1 cut(s)	1363
CseI	GACGC	4 cut(s)	127, 406, 847, 1308
Csp6I	GTAC	4 cut(s)	76, 766, 1498, 1552
CspCI	CAANNNNNGTGG	4 cut(s)	574, 609, 853, 888
CviAII	CATG	5 cut(s)	145, 316, 409, 787, 1540
CviQI	GTAC	4 cut(s)	76, 766, 1498, 1552
DdeI	CTNAG	9 cut(s)	257, 690, 769, 986, 993, 1062, 1086, 1347, 1494
DinI	GGCGCC	2 cut(s)	529, 582
DpnI	GATC	7 cut(s)	534, 626, 807, 1173, 1209, 1247, 1538
DpnII	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
DrdI	GACNNNNNNGTC	1 cut(s)	113
DseDI	GACNNNNNNGTC	1 cut(s)	113
EaeI	YGGCCR	1 cut(s)	195
EagI	CGGCCG	1 cut(s)	195
Eam1104I	CTCTTC	3 cut(s)	387, 1455, 1472
EarI	CTCTTC	3 cut(s)	387, 1455, 1472
EciI	GGCGGA	3 cut(s)	58, 620, 848
EclXI	CGGCCG	1 cut(s)	195
Eco24I	GRGCYC	1 cut(s)	372
Eco31I	GGTCTC	1 cut(s)	514
Eco32I	GATATC	1 cut(s)	610
Eco47I	GGWCC	4 cut(s)	49, 811, 1300, 1408
Eco52I	CGGCCG	1 cut(s)	195
Eco57I	CTGAAG	4 cut(s)	719, 866, 1250, 1496
EcoRII	CCWGG	3 cut(s)	387, 1272, 1302
EcoRV	GATATC	1 cut(s)	610
EcoT38I	GRGCYC	1 cut(s)	372
EgeI	GGCGCC	2 cut(s)	529, 582
EheI	GGCGCC	2 cut(s)	529, 582
Esp3I	CGTCTC	3 cut(s)	299, 389, 830
FaeI	CATG	5 cut(s)	148, 319, 412, 790, 1543
FaiI	YATR	9 cut(s)	146, 308, 317, 410, 423, 788, 981, 1001, 1541
FaqI	GGGAC	4 cut(s)	125, 559, 907, 1464
FatI	CATG	5 cut(s)	144, 315, 408, 786, 1539
FauI	CCCGC	1 cut(s)	1119
FbaI	TGATCA	1 cut(s)	1536
FblI	GTMKAC	1 cut(s)	123
FokI	GGATG	2 cut(s)	609, 1026
FriOI	GRGCYC	1 cut(s)	372
GlaI	GCGC	2 cut(s)	529, 582
HaeII	RGCGCY	2 cut(s)	531, 584
HaeIII	GGCC	6 cut(s)	113, 131, 197, 320, 654, 826
HapII	CCGG	6 cut(s)	353, 456, 815, 827, 1049, 1343
HgaI	GACGC	4 cut(s)	127, 406, 847, 1308
HhaI	GCGC	2 cut(s)	530, 583
Hin1I	GRCGYC	2 cut(s)	528, 581
Hin1II	CATG	5 cut(s)	148, 319, 412, 790, 1543
Hin6I	GCGC	2 cut(s)	528, 581
HinP1I	GCGC	2 cut(s)	528, 581
HincII	GTYRAC	2 cut(s)	178, 472
HindII	GTYRAC	2 cut(s)	178, 472
HpaII	CCGG	6 cut(s)	353, 456, 815, 827, 1049, 1343
HphI	GGTGA	6 cut(s)	268, 968, 1171, 1207, 1247, 1546
Hpy166II	GTNNAC	6 cut(s)	124, 178, 439, 472, 1513, 1554
Hpy188I	TCNGA	9 cut(s)	478, 667, 693, 699, 727, 1087, 1224, 1459, 1495
Hpy188III	TCNNGA	7 cut(s)	428, 635, 706, 754, 809, 934, 1249
Hpy8I	GTNNAC	6 cut(s)	124, 178, 439, 472, 1513, 1554
Hpy99I	CGWCG	4 cut(s)	119, 122, 164, 1324
HpyAV	CCTTC	8 cut(s)	283, 331, 350, 694, 1174, 1225, 1246, 1349
HpyCH4III	ACNGT	2 cut(s)	872, 898
HpyF10VI	GCNNNNNNNGC	5 cut(s)	161, 216, 267, 847, 1313
HpyF3I	CTNAG	9 cut(s)	257, 690, 769, 986, 993, 1062, 1086, 1347, 1494
Hsp92I	GRCGYC	2 cut(s)	528, 581
Hsp92II	CATG	5 cut(s)	148, 319, 412, 790, 1543
HspAI	GCGC	2 cut(s)	528, 581
KasI	GGCGCC	2 cut(s)	527, 580
KroI	GCCGGC	1 cut(s)	826
KroNI	GCCGGC	1 cut(s)	828
Ksp22I	TGATCA	1 cut(s)	1536
KspI	CCGCGG	1 cut(s)	1363
Kzo9I	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
LmnI	GCTCC	2 cut(s)	275, 1184
Lsp1109I	GCAGC	4 cut(s)	367, 828, 1303, 1458
LweI	GCATC	1 cut(s)	587
MaeIII	GTNAC	1 cut(s)	956
MalI	GATC	7 cut(s)	534, 626, 807, 1173, 1209, 1247, 1538
MboI	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
MhlI	GDGCHC	2 cut(s)	372, 1202
MluCI	AATT	7 cut(s)	53, 499, 638, 1018, 1382, 1392, 1402
Mly113I	GGCGCC	2 cut(s)	528, 581
MlyI	GAGTC	8 cut(s)	59, 223, 467, 695, 1073, 1097, 1358, 1499
MmeI	TCCRAC	5 cut(s)	42, 1221, 1380, 1467, 1509
MroNI	GCCGGC	1 cut(s)	826
MroXI	GAANNNNTTC	2 cut(s)	713, 1464
MseI	TTAA	1 cut(s)	489
MspA1I	CMGCKG	5 cut(s)	773, 1114, 1316, 1362, 1471
MspI	CCGG	6 cut(s)	353, 456, 815, 827, 1049, 1343
MspR9I	CCNGG	7 cut(s)	353, 389, 456, 815, 1049, 1274, 1304
MvaI	CCWGG	3 cut(s)	389, 1274, 1304
MvnI	CGCG	3 cut(s)	74, 219, 1362
MwoI	GCNNNNNNNGC	5 cut(s)	161, 216, 267, 847, 1313
NaeI	GCCGGC	1 cut(s)	828
NarI	GGCGCC	2 cut(s)	528, 581
NciI	CCSGG	4 cut(s)	353, 456, 815, 1049
NdeII	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
NgoMIV	GCCGGC	1 cut(s)	826
NlaIII	CATG	5 cut(s)	148, 319, 412, 790, 1543
NlaIV	GGNNCC	4 cut(s)	529, 582, 716, 813
NmeAIII	GCCGAG	1 cut(s)	627
NmuCI	GTSAC	1 cut(s)	956
NspV	TTCGAA	1 cut(s)	204
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PdiI	GCCGGC	1 cut(s)	828
PdmI	GAANNNNTTC	2 cut(s)	713, 1464
PfeI	GAWTC	2 cut(s)	694, 1219
PflFI	GACNNNGTC	1 cut(s)	232
PflMI	CCANNNNNTGG	1 cut(s)	1417
PfoI	TCCNGGA	3 cut(s)	351, 387, 1272
PleI	GAGTC	8 cut(s)	59, 223, 467, 694, 1072, 1096, 1357, 1498
PluTI	GGCGCC	2 cut(s)	531, 584
PpsI	GAGTC	8 cut(s)	59, 223, 467, 694, 1072, 1096, 1357, 1498
PshAI	GACNNNNGTC	1 cut(s)	291
Psp6I	CCWGG	3 cut(s)	387, 1272, 1302
PspGI	CCWGG	3 cut(s)	387, 1272, 1302
PspN4I	GGNNCC	4 cut(s)	529, 582, 716, 813
PspPI	GGNCC	5 cut(s)	49, 653, 811, 1300, 1408
PsrI	GAACNNNNNNTAC	2 cut(s)	749, 781
PstNI	CAGNNNCTG	3 cut(s)	697, 1228, 1474
PsyI	GACNNNGTC	1 cut(s)	232
PvuII	CAGCTG	2 cut(s)	1316, 1471
RsaI	GTAC	4 cut(s)	77, 767, 1499, 1553
RsaNI	GTAC	4 cut(s)	76, 766, 1498, 1552
SacII	CCGCGG	1 cut(s)	1363
SaqAI	TTAA	1 cut(s)	489
Sau3AI	GATC	7 cut(s)	532, 624, 805, 1171, 1207, 1245, 1536
Sau96I	GGNCC	5 cut(s)	49, 653, 811, 1300, 1408
ScaI	AGTACT	1 cut(s)	767
SchI	GAGTC	8 cut(s)	59, 223, 467, 695, 1073, 1097, 1358, 1499
ScrFI	CCNGG	7 cut(s)	353, 389, 456, 815, 1049, 1274, 1304
SduI	GDGCHC	2 cut(s)	372, 1202
SfaNI	GCATC	1 cut(s)	587
SfcI	CTRYAG	1 cut(s)	868
SfoI	GGCGCC	2 cut(s)	529, 582
Sfr303I	CCGCGG	1 cut(s)	1363
SfuI	TTCGAA	1 cut(s)	204
SgrBI	CCGCGG	1 cut(s)	1363
SinI	GGWCC	4 cut(s)	49, 811, 1300, 1408
Sse9I	AATT	7 cut(s)	53, 499, 638, 1018, 1382, 1392, 1402
SspDI	GGCGCC	2 cut(s)	527, 580
SspI	AATATT	1 cut(s)	1122
StyD4I	CCNGG	7 cut(s)	351, 387, 454, 813, 1047, 1272, 1302
TaaI	ACNGT	2 cut(s)	872, 898
TaqI	TCGA	2 cut(s)	14, 204
TasI	AATT	7 cut(s)	53, 499, 638, 1018, 1382, 1392, 1402
TatI	WGTACW	2 cut(s)	765, 1551
TauI	GCSGC	7 cut(s)	74, 654, 676, 833, 1101, 1104, 1365
TfiI	GAWTC	2 cut(s)	694, 1219
Tru1I	TTAA	1 cut(s)	489
Tru9I	TTAA	1 cut(s)	489
TscAI	CASTG	3 cut(s)	877, 1066, 1561
TseFI	GTSAC	1 cut(s)	956
TseI	GCWGC	4 cut(s)	380, 841, 1316, 1471
Tsp45I	GTSAC	1 cut(s)	956
TspDTI	ATGAA	3 cut(s)	161, 304, 996
TspGWI	ACGGA	2 cut(s)	163, 603
TspRI	CASTG	3 cut(s)	877, 1066, 1561
Tth111I	GACNNNGTC	1 cut(s)	232
Van91I	CCANNNNNTGG	1 cut(s)	1417
VpaK11BI	GGWCC	4 cut(s)	49, 811, 1300, 1408
XapI	RAATTY	2 cut(s)	1018, 1402
XcmI	CCANNNNNNNNNTGG	1 cut(s)	869
XmiI	GTMKAC	1 cut(s)	123
XmnI	GAANNNNTTC	2 cut(s)	713, 1464
ZrmI	AGTACT	1 cut(s)	767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.