Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005157

Physical Location & Seq

Genomic Coordinates Reverse (-)

1755745 .. 1757970

Gene Structure 2226 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1020600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGCGACTTCTTCGTCTTCTTCCACCCGCCAAGATAACCCACAAAAGGTCCAATTCCCGTTGGACTCGGCCGCGTATGAAAACCTCCAAGAAATTGGCTCTGGCGACAAGGCTGTCGTCTACAAGGCCGTCTGCATCCCCATGAACTCTGCAGTCGTCGCAATAAAGTCCGTCGACCTCGACCAATCCACGGCCGATTTCGAGAGCAAGCTCCGCGACACCAAGACTCTGTCTCTTTCCCACCCCAACATTCTCAGCAACCATTGCTCCTTCACCACCGCCGAGCGCCGTCTCTGGGTGGTCATGCCATTCATGGCCTTCGGCTCTCTGCAGTCCATAATAATCTCCTCCCCTGCTTTCCCTCAAGGATTACCAGAGCCCTGCATTGCTGTCTTCCTGAAAGAGACGCTAAACGCCATGTCGTATCTCCATGGCCAGGGACACACCCACGGCGACATCAAGCCCGGAAACATCTTGGTTGACTCTGATGGGTCCGTTAAGCTCGCCGATTTCGGGGTCTCTGCTTCTTTCTACGGCGCCGATCATTCAACTGGGACAAACGACACTTCGGCAACGCCAACTGGGACAAACGACACTTCGGCAATGCCAGATAGGGTGGCGCCCTACGGAGATGGATGCAAGGTGGATATCTGGGCATTTGGGATCATGGCACTGGAATTGGCTCGCGGCTGTCCTCCCCTGTCTGGCCTGCCACCGTTATTGGAATCTCAGATTCTAAAGGTCAAGAACAGGTACCAGTTTTCTGACTACGAAAACATCATCAAAGATTTCAAGAACAAGGAGTACTCTGTGGCTTTCAAGGACTTGGTGGGTTCTTGCCTTGATCAAGACCCAGCGAAGAGGCCGGCGTCAGAGACACTGCTGAAGCACTTGTTTTTCGATAACTGCAGGGGCAAGGATTTTCTGGTGGAGACTGTGCTTCGGCGTTTGGAGAGCGTTGAGGAGAGGTTCAAGAAAAGAAATAGGGGTTTGGGTGACTTGTTGGGCAAGGAAGAATATGGAGATGATCCAAAGAAAACAGAACACTTGCTCTCACTAGATGGAGCAAAAGACAGGCTGCATTTGTTCAAAGCAGATTTATTGGAAGAAGGATCTTTCGATGCTGCAGTTGATGGATGTGAAGCCGTTTTTCACACAGCATCACCTGTACTATTCTCGGCTACTGACCCACAGGCAGAACTAGTTAACACAGCCGTGAATGGAACGCTTGATGTTCTAAAACCATGTGTGAAATTTCCAACTGTCAAGAGAGGGGTACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010119 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1902456

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

428

Amino Acids

46.56

Weight (kDa)

5.45

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	26 - 300	1.1e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	29 - 298	2.2e-27	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14480
fragaria_vesca	FvH4_4g03000
malus_domestica	MD01G1106400.v1.1 MD01G1106800.v1.1 MD02G1095900.v1.1 MD04G1063400.v1.1 MD04G1063500.v1.1 MD07G1020600.v1.1 MD07G1020800.v1.1 MD16G1212100.v1.1
prunus_persica	Prupe.1G031800_v2.0.a1 Prupe.1G033800_v2.0.a1 Prupe.1G034000_v2.0.a1 Prupe.4G193100_v2.0.a1
pyrus_communis	pycom01g13320 pycom01g13330 pycom01g13350 pycom07g01600 pycom16g17810
rosa_chinensis	RchiOBHm_Chr4g0391181
rosa_laevigata	RLG00000009879
rosa_multiflora	Rmu_co8201760.1_g000001 Rmu_sc0000040.1_g000009
rosa_roxburghii	Rroxscaffold_5G00336980
rosa_rugosa	Rorug03G0334900
rosa_samantha	Rh4AG035700 Rh4BG030700 Rh4CG039000 Rh4DG033200
rosa_wichuraiana	Rw4G002800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	13, 113
Acc65I	GGTACC	1 cut(s)	753
AccB1I	GGYRCC	3 cut(s)	536, 619, 753
AccI	GTMKAC	2 cut(s)	120, 174
AccII	CGCG	3 cut(s)	74, 216, 687
AciI	CCGC	5 cut(s)	28, 72, 214, 279, 687
AclWI	GGATC	3 cut(s)	671, 1022, 1120
AcoI	YGGCCR	3 cut(s)	69, 192, 433
AcsI	RAATTY	1 cut(s)	1253
AcuI	CTGAAG	1 cut(s)	905
AcyI	GRCGYC	3 cut(s)	537, 620, 869
AfaI	GTAC	4 cut(s)	755, 806, 1170, 1278
AfiI	CCNNNNNNNGG	6 cut(s)	46, 190, 295, 436, 513, 704
AgsI	TTSAA	5 cut(s)	549, 793, 820, 973, 1090
AhlI	ACTAGT	1 cut(s)	1201
AjnI	CCWGG	1 cut(s)	435
AleI	CACNNNNGTG	1 cut(s)	1214
AluBI	AGCT	2 cut(s)	211, 502
AluI	AGCT	2 cut(s)	211, 502
Alw26I	GTCTC	6 cut(s)	237, 296, 398, 523, 869, 926
AlwI	GGATC	3 cut(s)	671, 1022, 1120
AoxI	GGCC	7 cut(s)	69, 126, 192, 315, 433, 706, 863
ApeKI	GCWGC	2 cut(s)	1078, 1124
ApoI	RAATTY	1 cut(s)	1253
Asp718I	GGTACC	1 cut(s)	753
AspLEI	GCGC	3 cut(s)	288, 539, 622
AspS9I	GGNCC	2 cut(s)	49, 492
AsuC2I	CCSGG	1 cut(s)	465
AsuHPI	GGTGA	3 cut(s)	265, 1007, 1155
AvaII	GGWCC	2 cut(s)	49, 492
BalI	TGGCCA	1 cut(s)	435
BanI	GGYRCC	3 cut(s)	536, 619, 753
BanII	GRGCYC	1 cut(s)	381
BbsI	GAAGAC	2 cut(s)	9, 385
BbvI	GCAGC	2 cut(s)	1065, 1111
BccI	CCATC	4 cut(s)	482, 626, 1055, 1127
BceAI	ACGGC	7 cut(s)	113, 207, 273, 466, 550, 1130, 1199
BciT130I	CCWGG	1 cut(s)	437
BclI	TGATCA	1 cut(s)	844
BcnI	CCSGG	1 cut(s)	465
BcoDI	GTCTC	6 cut(s)	237, 296, 398, 523, 869, 926
BcuI	ACTAGT	1 cut(s)	1201
BfaI	CTAG	2 cut(s)	1058, 1202
BfmI	CTRYAG	4 cut(s)	150, 329, 907, 1125
BfoI	RGCGCY	3 cut(s)	289, 540, 623
BisI	GCNGC	4 cut(s)	72, 688, 1079, 1125
BlsI	GCNGC	4 cut(s)	73, 689, 1080, 1126
BmcAI	AGTACT	1 cut(s)	806
Bme1390I	CCNGG	2 cut(s)	437, 465
Bme18I	GGWCC	2 cut(s)	49, 492
BmgT120I	GGNCC	2 cut(s)	49, 492
BmiI	GGNNCC	4 cut(s)	493, 538, 621, 755
BmrFI	CCNGG	2 cut(s)	437, 465
BmrI	ACTGGG	2 cut(s)	561, 591
BmsI	GCATC	4 cut(s)	144, 626, 1111, 1169
BmuI	ACTGGG	2 cut(s)	561, 591
BpiI	GAAGAC	2 cut(s)	9, 385
BpuEI	CTTGAG	1 cut(s)	348
BpuMI	CCSGG	1 cut(s)	465
BsaHI	GRCGYC	3 cut(s)	537, 620, 869
BsaI	GGTCTC	1 cut(s)	523
BsaJI	CCNNGG	4 cut(s)	189, 430, 436, 448
BsaXI	ACNNNNNCTCC	2 cut(s)	251, 281
Bsc4I	CCNNNNNNNGG	6 cut(s)	46, 190, 295, 436, 513, 704
Bse118I	RCCGGY	1 cut(s)	865
Bse1I	ACTGG	4 cut(s)	556, 586, 678, 757
Bse3DI	GCAATG	3 cut(s)	262, 384, 609
BseBI	CCWGG	1 cut(s)	437
BseDI	CCNNGG	4 cut(s)	189, 430, 436, 448
BseGI	GGATG	3 cut(s)	135, 641, 1142
BseLI	CCNNNNNNNGG	6 cut(s)	46, 190, 295, 436, 513, 704
BseMI	GCAATG	3 cut(s)	262, 384, 609
BseMII	CTCAG	2 cut(s)	268, 743
BseNI	ACTGG	4 cut(s)	556, 586, 678, 757
BseRI	GAGGAG	2 cut(s)	337, 977
BseX3I	CGGCCG	2 cut(s)	69, 192
BseXI	GCAGC	2 cut(s)	1065, 1111
BseYI	CCCAGC	1 cut(s)	853
Bsh1236I	CGCG	3 cut(s)	74, 216, 687
Bsh1285I	CGRYCG	2 cut(s)	72, 195
BshFI	GGCC	7 cut(s)	71, 128, 194, 317, 435, 708, 865
BshNI	GGYRCC	3 cut(s)	536, 619, 753
BsiEI	CGRYCG	2 cut(s)	72, 195
BsiSI	CCGG	2 cut(s)	465, 866
BslFI	GGGAC	3 cut(s)	453, 568, 598
BslI	CCNNNNNNNGG	6 cut(s)	46, 190, 295, 436, 513, 704
BsmAI	GTCTC	6 cut(s)	237, 296, 398, 523, 869, 926
BsmBI	CGTCTC	2 cut(s)	296, 398
BsmFI	GGGAC	3 cut(s)	453, 568, 598
BsnI	GGCC	7 cut(s)	71, 128, 194, 317, 435, 708, 865
Bso31I	GGTCTC	1 cut(s)	523
Bsp1286I	GDGCHC	1 cut(s)	381
Bsp143I	GATC	5 cut(s)	541, 663, 844, 1027, 1112
Bsp19I	CCATGG	1 cut(s)	430
BspACI	CCGC	5 cut(s)	28, 72, 214, 279, 687
BspANI	GGCC	7 cut(s)	71, 128, 194, 317, 435, 708, 865
BspCNI	CTCAG	2 cut(s)	267, 742
BspFNI	CGCG	3 cut(s)	74, 216, 687
BspLI	GGNNCC	4 cut(s)	493, 538, 621, 755
BspMAI	CTGCAG	4 cut(s)	154, 333, 911, 1129
BspPI	GGATC	3 cut(s)	671, 1022, 1120
BspT107I	GGYRCC	3 cut(s)	536, 619, 753
BspTNI	GGTCTC	1 cut(s)	523
BsrDI	GCAATG	3 cut(s)	262, 384, 609
BsrFI	RCCGGY	1 cut(s)	865
BsrI	ACTGG	4 cut(s)	556, 586, 678, 757
BssAI	RCCGGY	1 cut(s)	865
BssECI	CCNNGG	4 cut(s)	189, 430, 436, 448
BssMI	GATC	5 cut(s)	541, 663, 844, 1027, 1112
BssNI	GRCGYC	3 cut(s)	537, 620, 869
BssT1I	CCWWGG	1 cut(s)	430
Bst2UI	CCWGG	1 cut(s)	437
Bst4CI	ACNGT	4 cut(s)	717, 937, 1264, 1281
Bst6I	CTCTTC	1 cut(s)	854
BstACI	GRCGYC	3 cut(s)	537, 620, 869
BstAPI	GCANNNNNTGC	1 cut(s)	264
BstC8I	GCNNGC	5 cut(s)	209, 504, 685, 710, 867
BstDEI	CTNAG	2 cut(s)	254, 729
BstDSI	CCRYGG	3 cut(s)	189, 430, 448
BstF5I	GGATG	3 cut(s)	135, 641, 1142
BstFNI	CGCG	3 cut(s)	74, 216, 687
BstH2I	RGCGCY	3 cut(s)	289, 540, 623
BstHHI	GCGC	3 cut(s)	288, 539, 622
BstKTI	GATC	5 cut(s)	544, 666, 847, 1030, 1115
BstMAI	GTCTC	6 cut(s)	237, 296, 398, 523, 869, 926
BstMBI	GATC	5 cut(s)	541, 663, 844, 1027, 1112
BstMCI	CGRYCG	2 cut(s)	72, 195
BstMWI	GCNNNNNNNGC	3 cut(s)	158, 213, 264
BstNI	CCWGG	1 cut(s)	437
BstSCI	CCNGG	2 cut(s)	435, 463
BstSFI	CTRYAG	4 cut(s)	150, 329, 907, 1125
BstUI	CGCG	3 cut(s)	74, 216, 687
BstV1I	GCAGC	2 cut(s)	1065, 1111
BstV2I	GAAGAC	2 cut(s)	9, 385
BstX2I	RGATCY	1 cut(s)	1112
BstXI	CCANNNNNNTGG	1 cut(s)	95
BstYI	RGATCY	1 cut(s)	1112
BstZI	CGGCCG	2 cut(s)	69, 192
BsuRI	GGCC	7 cut(s)	71, 128, 194, 317, 435, 708, 865
BtgI	CCRYGG	3 cut(s)	189, 430, 448
BtsCI	GGATG	3 cut(s)	135, 641, 1142
BtsI	GCAGTG	1 cut(s)	878
BtsIMutI	CAGTG	2 cut(s)	671, 878
Cac8I	GCNNGC	5 cut(s)	209, 504, 685, 710, 867
CfoI	GCGC	3 cut(s)	288, 539, 622
Cfr10I	RCCGGY	1 cut(s)	865
Cfr13I	GGNCC	2 cut(s)	49, 492
CseI	GACGC	2 cut(s)	415, 858
Csp6I	GTAC	4 cut(s)	754, 805, 1169, 1277
CspCI	CAANNNNNGTGG	2 cut(s)	702, 737
CviAII	CATG	7 cut(s)	142, 304, 313, 418, 431, 667, 1245
CviQI	GTAC	4 cut(s)	754, 805, 1169, 1277
DdeI	CTNAG	2 cut(s)	254, 729
DinI	GGCGCC	2 cut(s)	538, 621
DpnI	GATC	5 cut(s)	543, 665, 846, 1029, 1114
DpnII	GATC	5 cut(s)	541, 663, 844, 1027, 1112
DrdI	GACNNNNNNGTC	2 cut(s)	13, 113
DseDI	GACNNNNNNGTC	2 cut(s)	13, 113
EaeI	YGGCCR	3 cut(s)	69, 192, 433
EagI	CGGCCG	2 cut(s)	69, 192
Eam1104I	CTCTTC	1 cut(s)	854
EarI	CTCTTC	1 cut(s)	854
EclXI	CGGCCG	2 cut(s)	69, 192
Eco130I	CCWWGG	1 cut(s)	430
Eco24I	GRGCYC	1 cut(s)	381
Eco31I	GGTCTC	1 cut(s)	523
Eco32I	GATATC	1 cut(s)	649
Eco47I	GGWCC	2 cut(s)	49, 492
Eco52I	CGGCCG	2 cut(s)	69, 192
Eco57I	CTGAAG	1 cut(s)	905
EcoRII	CCWGG	1 cut(s)	435
EcoRV	GATATC	1 cut(s)	649
EcoT14I	CCWWGG	1 cut(s)	430
EcoT38I	GRGCYC	1 cut(s)	381
EgeI	GGCGCC	2 cut(s)	538, 621
EheI	GGCGCC	2 cut(s)	538, 621
ErhI	CCWWGG	1 cut(s)	430
Esp3I	CGTCTC	2 cut(s)	296, 398
FaeI	CATG	7 cut(s)	145, 307, 316, 421, 434, 670, 1248
FaqI	GGGAC	3 cut(s)	453, 568, 598
FatI	CATG	7 cut(s)	141, 303, 312, 417, 430, 666, 1244
FauI	CCCGC	1 cut(s)	35
FbaI	TGATCA	1 cut(s)	844
FblI	GTMKAC	2 cut(s)	120, 174
Fnu4HI	GCNGC	4 cut(s)	72, 688, 1079, 1125
FokI	GGATG	3 cut(s)	122, 648, 1149
FriOI	GRGCYC	1 cut(s)	381
Fsp4HI	GCNGC	4 cut(s)	72, 688, 1079, 1125
FspBI	CTAG	2 cut(s)	1058, 1202
GlaI	GCGC	3 cut(s)	287, 538, 621
GluI	GCNGC	4 cut(s)	72, 688, 1079, 1125
GsaI	CCCAGC	1 cut(s)	857
HaeII	RGCGCY	3 cut(s)	289, 540, 623
HaeIII	GGCC	7 cut(s)	71, 128, 194, 317, 435, 708, 865
HapII	CCGG	2 cut(s)	465, 866
HgaI	GACGC	2 cut(s)	415, 858
HhaI	GCGC	3 cut(s)	288, 539, 622
Hin1I	GRCGYC	3 cut(s)	537, 620, 869
Hin1II	CATG	7 cut(s)	145, 307, 316, 421, 434, 670, 1248
Hin6I	GCGC	3 cut(s)	286, 537, 620
HinP1I	GCGC	3 cut(s)	286, 537, 620
HincII	GTYRAC	3 cut(s)	175, 481, 1207
HindII	GTYRAC	3 cut(s)	175, 481, 1207
HinfI	GANTC	5 cut(s)	65, 226, 482, 725, 733
HpaI	GTTAAC	1 cut(s)	1207
HpaII	CCGG	2 cut(s)	465, 866
HphI	GGTGA	3 cut(s)	265, 1007, 1155
Hpy166II	GTNNAC	4 cut(s)	121, 175, 481, 1207
Hpy188I	TCNGA	4 cut(s)	487, 732, 766, 874
Hpy188III	TCNNGA	7 cut(s)	202, 397, 745, 793, 848, 973, 1267
Hpy8I	GTNNAC	4 cut(s)	121, 175, 481, 1207
Hpy99I	CGWCG	2 cut(s)	161, 176
HpyAV	CCTTC	3 cut(s)	280, 328, 1103
HpyCH4III	ACNGT	4 cut(s)	717, 937, 1264, 1281
HpyCH4V	TGCA	8 cut(s)	135, 152, 331, 384, 639, 909, 1081, 1127
HpyF10VI	GCNNNNNNNGC	3 cut(s)	158, 213, 264
HpyF3I	CTNAG	2 cut(s)	254, 729
Hsp92I	GRCGYC	3 cut(s)	537, 620, 869
Hsp92II	CATG	7 cut(s)	145, 307, 316, 421, 434, 670, 1248
HspAI	GCGC	3 cut(s)	286, 537, 620
KasI	GGCGCC	2 cut(s)	536, 619
KpnI	GGTACC	1 cut(s)	757
KroI	GCCGGC	1 cut(s)	865
KroNI	GCCGGC	1 cut(s)	867
Ksp22I	TGATCA	1 cut(s)	844
KspAI	GTTAAC	1 cut(s)	1207
Kzo9I	GATC	5 cut(s)	541, 663, 844, 1027, 1112
LmnI	GCTCC	3 cut(s)	216, 272, 1064
Lsp1109I	GCAGC	2 cut(s)	1065, 1111
LweI	GCATC	4 cut(s)	144, 626, 1111, 1169
MaeI	CTAG	2 cut(s)	1058, 1202
MaeIII	GTNAC	1 cut(s)	995
MalI	GATC	5 cut(s)	543, 665, 846, 1029, 1114
MboI	GATC	5 cut(s)	541, 663, 844, 1027, 1112
MboII	GAAGA	7 cut(s)	3, 9, 12, 385, 871, 1025, 1118
MflI	RGATCY	1 cut(s)	1112
MhlI	GDGCHC	1 cut(s)	381
MlsI	TGGCCA	1 cut(s)	435
MluCI	AATT	4 cut(s)	53, 93, 677, 1253
MluNI	TGGCCA	1 cut(s)	435
Mly113I	GGCGCC	2 cut(s)	537, 620
MlyI	GAGTC	3 cut(s)	59, 220, 476
MmeI	TCCRAC	2 cut(s)	42, 1283
MnlI	CCTC	9 cut(s)	95, 188, 358, 372, 705, 855, 955, 960, 1265
Mox20I	TGGCCA	1 cut(s)	435
MroNI	GCCGGC	1 cut(s)	865
MscI	TGGCCA	1 cut(s)	435
MseI	TTAA	2 cut(s)	498, 1206
MslI	CAYNNNNRTG	2 cut(s)	140, 1214
Msp20I	TGGCCA	1 cut(s)	435
MspI	CCGG	2 cut(s)	465, 866
MspR9I	CCNGG	2 cut(s)	437, 465
MvaI	CCWGG	1 cut(s)	437
MvnI	CGCG	3 cut(s)	74, 216, 687
MwoI	GCNNNNNNNGC	3 cut(s)	158, 213, 264
NaeI	GCCGGC	1 cut(s)	867
NarI	GGCGCC	2 cut(s)	537, 620
NciI	CCSGG	1 cut(s)	465
NcoI	CCATGG	1 cut(s)	430
NdeII	GATC	5 cut(s)	541, 663, 844, 1027, 1112
NgoMIV	GCCGGC	1 cut(s)	865
NlaIII	CATG	7 cut(s)	145, 307, 316, 421, 434, 670, 1248
NlaIV	GGNNCC	4 cut(s)	493, 538, 621, 755
NmeAIII	GCCGAG	3 cut(s)	47, 307, 1157
NmuCI	GTSAC	1 cut(s)	995
OliI	CACNNNNGTG	1 cut(s)	1214
PdiI	GCCGGC	1 cut(s)	867
PfeI	GAWTC	2 cut(s)	725, 733
PflFI	GACNNNGTC	1 cut(s)	229
PkrI	GCNGC	4 cut(s)	73, 689, 1080, 1126
PleI	GAGTC	3 cut(s)	59, 220, 476
PluTI	GGCGCC	2 cut(s)	540, 623
PpsI	GAGTC	3 cut(s)	59, 220, 476
Psp6I	CCWGG	1 cut(s)	435
PspFI	CCCAGC	1 cut(s)	853
PspGI	CCWGG	1 cut(s)	435
PspN4I	GGNNCC	4 cut(s)	493, 538, 621, 755
PspPI	GGNCC	2 cut(s)	49, 492
PsrI	GAACNNNNNNTAC	2 cut(s)	788, 820
PstI	CTGCAG	4 cut(s)	154, 333, 911, 1129
PsuI	RGATCY	1 cut(s)	1112
PsyI	GACNNNGTC	1 cut(s)	229
RsaI	GTAC	4 cut(s)	755, 806, 1170, 1278
RsaNI	GTAC	4 cut(s)	754, 805, 1169, 1277
RseI	CAYNNNNRTG	2 cut(s)	140, 1214
SalI	GTCGAC	1 cut(s)	173
SaqAI	TTAA	2 cut(s)	498, 1206
SatI	GCNGC	4 cut(s)	72, 688, 1079, 1125
Sau3AI	GATC	5 cut(s)	541, 663, 844, 1027, 1112
Sau96I	GGNCC	2 cut(s)	49, 492
ScaI	AGTACT	1 cut(s)	806
SchI	GAGTC	3 cut(s)	59, 220, 476
ScrFI	CCNGG	2 cut(s)	437, 465
SduI	GDGCHC	1 cut(s)	381
SfaNI	GCATC	4 cut(s)	144, 626, 1111, 1169
SfcI	CTRYAG	4 cut(s)	150, 329, 907, 1125
SfoI	GGCGCC	2 cut(s)	538, 621
SinI	GGWCC	2 cut(s)	49, 492
SmiMI	CAYNNNNRTG	2 cut(s)	140, 1214
SmlI	CTYRAG	1 cut(s)	363
SmoI	CTYRAG	1 cut(s)	363
SpeI	ACTAGT	1 cut(s)	1201
Sse9I	AATT	4 cut(s)	53, 93, 677, 1253
SsiI	CCGC	5 cut(s)	28, 72, 214, 279, 687
SspDI	GGCGCC	2 cut(s)	536, 619
SspMI	CTAG	2 cut(s)	1058, 1202
StyD4I	CCNGG	2 cut(s)	435, 463
StyI	CCWWGG	1 cut(s)	430
TaaI	ACNGT	4 cut(s)	717, 937, 1264, 1281
TaqI	TCGA	5 cut(s)	174, 180, 201, 900, 1119
TasI	AATT	4 cut(s)	53, 93, 677, 1253
TatI	WGTACW	2 cut(s)	804, 1168
TauI	GCSGC	2 cut(s)	74, 690
TfiI	GAWTC	2 cut(s)	725, 733
Tru1I	TTAA	2 cut(s)	498, 1206
Tru9I	TTAA	2 cut(s)	498, 1206
TscAI	CASTG	2 cut(s)	678, 885
TseFI	GTSAC	1 cut(s)	995
TseI	GCWGC	2 cut(s)	1078, 1124
Tsp45I	GTSAC	1 cut(s)	995
TspDTI	ATGAA	3 cut(s)	93, 158, 301
TspGWI	ACGGA	3 cut(s)	160, 484, 642
TspRI	CASTG	2 cut(s)	678, 885
Tth111I	GACNNNGTC	1 cut(s)	229
VpaK11BI	GGWCC	2 cut(s)	49, 492
XapI	RAATTY	1 cut(s)	1253
XmiI	GTMKAC	2 cut(s)	120, 174
XspI	CTAG	2 cut(s)	1058, 1202
ZrmI	AGTACT	1 cut(s)	806

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.