MD01G1111100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008794
Physical Location & Seq
Reverse (-)
22365151 .. 22370674
5524 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1111100.v1.1.491

Sequence Viewer

Length: 1419 bp
ATGTCACTGTCAGGGTGTGAAATGGAGAACCCAGAAGCAAATCAGCTTCCTGAAGTTGATTCTTTGCCTGATGGGTTTGTTGTGAGCTCCCCAGAGCCGCTGGCTCCGGCAACTCCAACCGTAGAACAAGAGAAGCCGTTGGGTAACAACGAGAATGATAATTCTACAGAAAGGGATAGTTCAAAGGAACCTGTAGGTGAATCGGCTGCAAATGAGTTTCAGCCAAGCCAGGATAGGGCAGAGAAGACTCAGAAACTGAGAACGTTTCCTGTTCTGTTGTCTGAAACAGATAGTTCTCATGTTTCAGTAGAATCAGTTCAGGTTCCCAAAGATGCCTCTGCCGAGCAGACAGAAGAAGGTACACTCGTCATGCCTGATTCATCTGTTTCTGTTTCTGAGGCTTCGGTTGGGCTATCTGTAGGCGGTGAGGTAAAGGAGGAACAAGTTGAAGCAAGATGTCAAAGTTCAGAGAGACCTGAAGGAGGCTCAGATGCGTCACCCACCAATTTGAAGGAAACGTCCTCATTGGAGAGTGTTGGAACTTTGAAAAACAGAAAAACAGAATCCACTGAAACCAAACGTAAGAGTGTAAAGCGTACCTTTAAATCTGAAAAGGAGTTCATAGAATTCACCTTGAAGTATCAACAAGCTCTTGCTGAAAGAGATTCGGCCATTTCTGTTCGAGATAAGCTTGAGTCCTTGTGCAGGGAGTTACAACGCCAGAACAAAGTATTAATGGATGAGTGCAAACGGGTGTCAACGGAGGGGCAGAACTTGAGATTAGATTTATCAGTCAAGTTCCAAGATGCAATAAAGGATGTGAGCACTAAATTGGATGAGCAAAAGGAAGAATGTATCTCTCAGCTAAAGGAGAATGAAATGTTAAGAACTAAGCTCCAGCAGCTCACTTATCAGCACACTCTTTCTGAACAGCAATATGAACAGAAGTTAAAGCAGAAATCACTGGAGCTTCAAATTGCTGATTTGAAGACTAAGCAGCATGAAGAGAAATTACTCCAGGAACAGTCCCAGATGAAATTATATGCGGAACAGGTGTCACAGTTATTATCAACTGAAAAGAATTTGCGCTTACAGTTGACAGCTGATGGAGAGAAATTCCAACAGTTTCAGGATGCATTGTTGAAAAGCAATGAAGTTTTTGAGACATTTAAACAGGAGATTGAAAAGATGGCAAAGTCCATCAAGGAACTCAAGAAGGAAAACTTATTCTTGAAGAGCAAATGTGAGAAAACAGATGTCACTCTTATAGAACTTGTAGACGAGCGTGAACGGTTGAAGAAACAGTTGGAGAAAACAAAGAACCAGAAAGAAAAGCTTGAATCATTGTGCCGGTCACTTCAAGCAGAAAGGAAACAGAACTCCACTGGGAGCAACAACTCCGATTCTGTTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Taxilin

Protein Analysis

473

Amino Acids

53.38

Weight (kDa)

5.08

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Taxilin PF09728 171 - 456 7.5e-74 Myosin-like coiled-coil protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1278
AciI CCGC 3 cut(s) 98, 423, 1046
AclI AACGTT 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 3 cut(s) 626, 1081, 1115
AcuI CTGAAG 2 cut(s) 72, 498
AfaI GTAC 2 cut(s) 361, 598
AfiI CCNNNNNNNGG 3 cut(s) 235, 482, 705
AjnI CCWGG 2 cut(s) 228, 1017
AjuI GAANNNNNNNTTGG 2 cut(s) 1289, 1321
Alw21I GWGCWC 2 cut(s) 89, 827
Alw26I GTCTC 2 cut(s) 466, 1157
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 1 cut(s) 669
ApeKI GCWGC 3 cut(s) 206, 901, 997
ApoI RAATTY 3 cut(s) 626, 1081, 1115
AseI ATTAAT 1 cut(s) 734
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 1089
AsuHPI GGTGA 4 cut(s) 209, 437, 489, 622
BaeI ACNNNNGTAYC 2 cut(s) 351, 384
BanII GRGCYC 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 996, 1028
BbsI GAAGAC 2 cut(s) 251, 995
Bbv12I GWGCWC 2 cut(s) 89, 827
BbvI GCAGC 3 cut(s) 193, 913, 1009
BccI CCATC 4 cut(s) 65, 1100, 1183, 1208
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 230, 1019
BcoDI GTCTC 2 cut(s) 466, 1157
BfmI CTRYAG 3 cut(s) 165, 192, 417
BisI GCNGC 4 cut(s) 98, 207, 902, 998
BlsI GCNGC 4 cut(s) 99, 208, 903, 999
Bme1390I CCNGG 2 cut(s) 230, 1019
BmiI GGNNCC 3 cut(s) 105, 189, 324
BmrFI CCNGG 2 cut(s) 230, 1019
BmrI ACTGGG 1 cut(s) 1395
BmsI GCATC 4 cut(s) 322, 481, 796, 1123
BmuI ACTGGG 1 cut(s) 1395
BpiI GAAGAC 2 cut(s) 251, 995
BpmI CTGGAG 3 cut(s) 881, 986, 1001
BpuEI CTTGAG 3 cut(s) 713, 796, 1196
BsaI GGTCTC 1 cut(s) 466
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 482, 705
Bse118I RCCGGY 1 cut(s) 1350
Bse1I ACTGG 2 cut(s) 969, 1390
Bse3DI GCAATG 1 cut(s) 1156
BseBI CCWGG 2 cut(s) 230, 1019
BseGI GGATG 4 cut(s) 745, 823, 841, 1138
BseLI CCNNNNNNNGG 3 cut(s) 235, 482, 705
BseMI GCAATG 1 cut(s) 1156
BseMII CTCAG 5 cut(s) 248, 263, 387, 501, 875
BseNI ACTGG 2 cut(s) 969, 1390
BseXI GCAGC 3 cut(s) 193, 913, 1009
BsgI GTGCAG 1 cut(s) 724
BshFI GGCC 1 cut(s) 671
BsiHKAI GWGCWC 2 cut(s) 89, 827
BsiSI CCGG 2 cut(s) 107, 1351
BslFI GGGAC 1 cut(s) 1012
BslI CCNNNNNNNGG 3 cut(s) 235, 482, 705
BsmAI GTCTC 2 cut(s) 466, 1157
BsmFI GGGAC 1 cut(s) 1012
BsnI GGCC 1 cut(s) 671
Bso31I GGTCTC 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 89, 827
BspACI CCGC 3 cut(s) 98, 423, 1046
BspANI GGCC 1 cut(s) 671
BspCNI CTCAG 5 cut(s) 249, 262, 388, 500, 874
BspLI GGNNCC 3 cut(s) 105, 189, 324
BspQI GCTCTTC 1 cut(s) 1229
BspTNI GGTCTC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 1156
BsrFI RCCGGY 1 cut(s) 1350
BsrI ACTGG 2 cut(s) 969, 1390
BssAI RCCGGY 1 cut(s) 1350
Bst2UI CCWGG 2 cut(s) 230, 1019
Bst4CI ACNGT 8 cut(s) 9, 121, 1026, 1062, 1095, 1125, 1293, 1305
Bst6I CTCTTC 2 cut(s) 999, 1229
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 7 cut(s) 249, 257, 396, 487, 861, 891, 993
BstENI CCTNNNNNAGG 2 cut(s) 480, 703
BstF5I GGATG 4 cut(s) 745, 823, 841, 1138
BstHHI GCGC 1 cut(s) 1089
BstMAI GTCTC 2 cut(s) 466, 1157
BstMWI GCNNNNNNNGC 1 cut(s) 901
BstNI CCWGG 2 cut(s) 230, 1019
BstSCI CCNGG 2 cut(s) 228, 1017
BstSFI CTRYAG 3 cut(s) 165, 192, 417
BstV1I GCAGC 3 cut(s) 193, 913, 1009
BstV2I GAAGAC 2 cut(s) 251, 995
BsuRI GGCC 1 cut(s) 671
BtsCI GGATG 4 cut(s) 745, 823, 841, 1138
BtsIMutI CAGTG 4 cut(s) 5, 567, 962, 1383
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 256
CfoI GCGC 1 cut(s) 1089
Cfr10I RCCGGY 1 cut(s) 1350
CseI GACGC 1 cut(s) 483
Csp6I GTAC 2 cut(s) 360, 597
CviAII CATG 3 cut(s) 299, 370, 1001
CviQI GTAC 2 cut(s) 360, 597
DdeI CTNAG 7 cut(s) 249, 257, 396, 487, 861, 891, 993
DraI TTTAAA 2 cut(s) 604, 1171
EaeI YGGCCR 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 999, 1229
EarI CTCTTC 2 cut(s) 999, 1229
Ecl136II GAGCTC 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 72, 498
EcoICRI GAGCTC 1 cut(s) 87
EcoNI CCTNNNNNAGG 2 cut(s) 480, 703
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 2 cut(s) 228, 1017
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 89
FaeI CATG 3 cut(s) 302, 373, 1004
FaiI YATR 8 cut(s) 300, 371, 623, 939, 1002, 1042, 1044, 1268
FalI AAGNNNNNCTT 6 cut(s) 584, 616, 1208, 1240, 1320, 1352
FaqI GGGAC 1 cut(s) 1012
FatI CATG 3 cut(s) 298, 369, 1000
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 4 cut(s) 98, 207, 902, 998
FokI GGATG 4 cut(s) 752, 830, 848, 1145
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 4 cut(s) 98, 207, 902, 998
GlaI GCGC 1 cut(s) 1088
GluI GCNGC 4 cut(s) 98, 207, 902, 998
GsuI CTGGAG 3 cut(s) 881, 986, 1001
HaeIII GGCC 1 cut(s) 671
HapII CCGG 2 cut(s) 107, 1351
HgaI GACGC 1 cut(s) 483
HhaI GCGC 1 cut(s) 1089
Hin1II CATG 3 cut(s) 302, 373, 1004
Hin6I GCGC 1 cut(s) 1087
HinP1I GCGC 1 cut(s) 1087
HincII GTYRAC 2 cut(s) 759, 1098
HindII GTYRAC 2 cut(s) 759, 1098
HindIII AAGCTT 2 cut(s) 689, 1334
HpaII CCGG 2 cut(s) 107, 1351
HphI GGTGA 4 cut(s) 209, 437, 489, 622
Hpy166II GTNNAC 5 cut(s) 362, 759, 1098, 1279, 1289
Hpy188I TCNGA 8 cut(s) 252, 283, 397, 469, 490, 610, 928, 1402
Hpy188III TCNNGA 6 cut(s) 50, 683, 1130, 1213, 1231, 1416
Hpy8I GTNNAC 5 cut(s) 362, 759, 1098, 1279, 1289
HpyAV CCTTC 4 cut(s) 350, 473, 505, 1210
HpyCH4III ACNGT 8 cut(s) 9, 121, 1026, 1062, 1095, 1125, 1293, 1305
HpyCH4IV ACGT 3 cut(s) 263, 518, 580
HpyCH4V TGCA 5 cut(s) 209, 705, 747, 809, 1136
HpyF10VI GCNNNNNNNGC 1 cut(s) 901
HpyF3I CTNAG 7 cut(s) 249, 257, 396, 487, 861, 891, 993
HpySE526I ACGT 3 cut(s) 263, 518, 580
Hsp92II CATG 3 cut(s) 302, 373, 1004
HspAI GCGC 1 cut(s) 1087
LguI GCTCTTC 1 cut(s) 1229
LmnI GCTCC 5 cut(s) 92, 109, 900, 967, 1389
Lsp1109I GCAGC 3 cut(s) 193, 913, 1009
LweI GCATC 4 cut(s) 322, 481, 796, 1123
MaeII ACGT 3 cut(s) 263, 518, 580
MaeIII GTNAC 7 cut(s) 3, 143, 495, 711, 1056, 1258, 1353
MboII GAAGA 7 cut(s) 256, 365, 860, 1000, 1016, 1246, 1309
MhlI GDGCHC 2 cut(s) 89, 827
MluCI AATT 9 cut(s) 160, 505, 626, 830, 975, 1010, 1037, 1081, 1115
MlyI GAGTC 2 cut(s) 241, 704
MmeI TCCRAC 4 cut(s) 140, 517, 1144, 1287
MnlI CCTC 7 cut(s) 346, 391, 421, 430, 476, 532, 757
Mph1103I ATGCAT 1 cut(s) 1138
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 5 cut(s) 603, 734, 884, 950, 1170
MspA1I CMGCKG 2 cut(s) 100, 1103
MspI CCGG 2 cut(s) 107, 1351
MspR9I CCNGG 2 cut(s) 230, 1019
MvaI CCWGG 2 cut(s) 230, 1019
MwoI GCNNNNNNNGC 1 cut(s) 901
NlaIII CATG 3 cut(s) 302, 373, 1004
NlaIV GGNNCC 3 cut(s) 105, 189, 324
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 5 cut(s) 3, 495, 1056, 1258, 1353
NsiI ATGCAT 1 cut(s) 1138
PciSI GCTCTTC 1 cut(s) 1229
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 8 cut(s) 59, 200, 311, 377, 563, 665, 1340, 1403
PfoI TCCNGGA 1 cut(s) 1017
PkrI GCNGC 4 cut(s) 99, 208, 903, 999
PleI GAGTC 2 cut(s) 241, 703
PpsI GAGTC 2 cut(s) 241, 703
PshBI ATTAAT 1 cut(s) 734
Psp124BI GAGCTC 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 228, 1017
PspGI CCWGG 2 cut(s) 228, 1017
PspN4I GGNNCC 3 cut(s) 105, 189, 324
PsrI GAACNNNNNNTAC 2 cut(s) 300, 332
PstNI CAGNNNCTG 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 2 cut(s) 361, 598
RsaNI GTAC 2 cut(s) 360, 597
SacI GAGCTC 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 1229
SaqAI TTAA 5 cut(s) 603, 734, 884, 950, 1170
SatI GCNGC 4 cut(s) 98, 207, 902, 998
SchI GAGTC 2 cut(s) 241, 704
ScrFI CCNGG 2 cut(s) 230, 1019
SduI GDGCHC 2 cut(s) 89, 827
SfaNI GCATC 4 cut(s) 322, 481, 796, 1123
SfcI CTRYAG 3 cut(s) 165, 192, 417
SmlI CTYRAG 3 cut(s) 692, 775, 1211
SmoI CTYRAG 3 cut(s) 692, 775, 1211
Sse9I AATT 9 cut(s) 160, 505, 626, 830, 975, 1010, 1037, 1081, 1115
SsiI CCGC 3 cut(s) 98, 423, 1046
SstI GAGCTC 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 228, 1017
TaaI ACNGT 8 cut(s) 9, 121, 1026, 1062, 1095, 1125, 1293, 1305
TaiI ACGT 3 cut(s) 266, 521, 583
TaqI TCGA 1 cut(s) 682
TasI AATT 9 cut(s) 160, 505, 626, 830, 975, 1010, 1037, 1081, 1115
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 8 cut(s) 59, 200, 311, 377, 563, 665, 1340, 1403
Tru1I TTAA 5 cut(s) 603, 734, 884, 950, 1170
Tru9I TTAA 5 cut(s) 603, 734, 884, 950, 1170
TscAI CASTG 4 cut(s) 12, 574, 969, 1390
TseFI GTSAC 5 cut(s) 3, 495, 1056, 1258, 1353
TseI GCWGC 3 cut(s) 206, 901, 997
Tsp45I GTSAC 5 cut(s) 3, 495, 1056, 1258, 1353
TspDTI ATGAA 7 cut(s) 369, 610, 891, 954, 1017, 1049, 1167
TspGWI ACGGA 1 cut(s) 776
TspRI CASTG 4 cut(s) 12, 574, 969, 1390
VspI ATTAAT 1 cut(s) 734
XagI CCTNNNNNAGG 2 cut(s) 480, 703
XapI RAATTY 3 cut(s) 626, 1081, 1115
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.