MD07G1177300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008794
Physical Location & Seq
Reverse (-)
25648818 .. 25654331
5514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1177300.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGGTACCAGGGTGAGAATGGAGAACCCAGAAGCAAACCAGCTTCCTGAAGTTGATTCATTGCCTGATGGGTTTGTTGAGAGCTCCCCAGAGCCACTGGCTCCAGCAACTCCAACCTTCGAGCAAGAGAAGCCGTTGGGTAACAACGAGAGTGATAATTCCACAGAAAGGGATAGTTCAAAGGAACCTGTAGGTGAATTGGCTGCAGATGAGTTTCAAGCAAGCCAGAGTAGGGCAGAGAAGACTCAGAAACTGAGAACATTTCCCGTTCCGTTGTCTGAAACAGATAGTTCTGATGTTTTAGTAGATTCAGTTCAAGTTCCCAAAAATGCCTCTACGGAGCATAGAGAAGAAGGTTCATTAGTCATGCCCGATTCAGCTGTTTCTGTTTCTGAGGCTTCGGGTGGGGTATCCGAAGGCGGTGAGGGAAAGAAACAAGTTGAAACAAGATGTCAAAGTTCAGAGAGACCTGAAGGGGGCTCAGATGCGCCAGCAACCAATTTGAAGGATAAGTCTTCATTGGAGAGTGTTGAAACTTTGAAAAACAGAAAAACAGAATCCACTGAAACCAAACGTAAGAGTGTAAAGCGTACCTTTAAATCAGAAAAGGAGTTCATAGAGTTCACTTTGAAGTATCAACAAGCTCTTGCCGAAAGAGATTCGGCCATTTCTGTTCGAGATAAGCTTGAGTCCTTGTGCAGGGAGTTACAACGCCAGAACAAAGTACTAATGGATGAGTGCAAACGGGTGTCCACAGAGGGGCAGAACTTGAGATTAGATTTATCAGTCAAGTTCCAAGATGCAATAAAGGATGTGAGCAATAAGCTGGATGAGCAAAAGGAAGAATGTATCTCTCAGCTAAAGGAGAATGAAATGTTAAGAACTAAGCTACAGCAGCTCAACTATCAGCACGCTCTTTCTGAACAGCAATATGAACAGATGTTAAAGCAGAAATCACTGGAGCTTCAAATTGCTGATTTGAAGACTAAGCAGCATGAAGAGAAATTACTCCAGGAACAGTCCCAGATGAAATTATATGCAGAACAGGTGTCACAGTTACTATCAACTGAAAAGAATTTGCGCTTACAGTTGACGGCTGATGGAGAGAAGTTCCAACAATTTCAGGACGCATTGTTGAAAAGCAATGAAGTTTTCGAGACATTTAAACAGGAGATTGAAAAGATGGCAAAGTCCATCAAGGAACTCAAGAAGGAAAACTTATTCTTGAAGAGCAAATGTGAGAAAACAGATGTCACTCTTATAGAACTCGTAGACGAGCGTGAACGATTGAAGAAACAGTTGGAGAAAACAAAGAACCAGAAAGAAAAGCTTGAATCACTGTGCCGGTCACTTCAAGCAGAAAGGAAACAGAACTCCACTGGGAGCAACAACTCCGATTCGGTTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Taxilin

Protein Analysis

471

Amino Acids

53.34

Weight (kDa)

5.23

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Taxilin PF09728 171 - 454 7.4e-74 Myosin-like coiled-coil protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 1272
AciI CCGC 1 cut(s) 420
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 1 cut(s) 1075
AcuI CTGAAG 2 cut(s) 69, 492
AfaI GTAC 3 cut(s) 7, 592, 726
AfiI CCNNNNNNNGG 5 cut(s) 168, 232, 476, 699, 759
AjnI CCWGG 2 cut(s) 8, 1011
AjuI GAANNNNNNNTTGG 2 cut(s) 1283, 1315
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 2 cut(s) 460, 1151
AlwNI CAGNNNCTG 1 cut(s) 253
AoxI GGCC 1 cut(s) 663
ApeKI GCWGC 3 cut(s) 203, 895, 991
ApoI RAATTY 1 cut(s) 1075
Asp718I GGTACC 1 cut(s) 5
AspLEI GCGC 2 cut(s) 490, 1083
AsuHPI GGTGA 3 cut(s) 25, 206, 434
BaeI ACNNNNGTAYC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 2 cut(s) 86, 482
BarI GAAGNNNNNNTAC 2 cut(s) 990, 1022
BbsI GAAGAC 3 cut(s) 248, 507, 989
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 3 cut(s) 190, 907, 1003
BccI CCATC 4 cut(s) 62, 1094, 1177, 1202
BceAI ACGGC 2 cut(s) 118, 1110
BciT130I CCWGG 2 cut(s) 10, 1013
BciVI GTATCC 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 460, 1151
BfmI CTRYAG 3 cut(s) 189, 204, 890
BfuI GTATCC 1 cut(s) 421
BisI GCNGC 3 cut(s) 204, 896, 992
BlsI GCNGC 3 cut(s) 205, 897, 993
BmcAI AGTACT 1 cut(s) 726
Bme1390I CCNGG 2 cut(s) 10, 1013
BmiI GGNNCC 4 cut(s) 7, 102, 186, 1404
BmrFI CCNGG 2 cut(s) 10, 1013
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 2 cut(s) 475, 790
BmuI ACTGGG 1 cut(s) 1389
BpiI GAAGAC 3 cut(s) 248, 507, 989
BpmI CTGGAG 3 cut(s) 87, 980, 995
BpuEI CTTGAG 3 cut(s) 707, 790, 1190
BsaI GGTCTC 1 cut(s) 460
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 232, 476, 699, 759
Bse118I RCCGGY 1 cut(s) 1344
Bse1I ACTGG 3 cut(s) 102, 963, 1384
Bse3DI GCAATG 2 cut(s) 59, 1150
BseBI CCWGG 2 cut(s) 10, 1013
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 3 cut(s) 739, 817, 835
BseLI CCNNNNNNNGG 5 cut(s) 168, 232, 476, 699, 759
BseMI GCAATG 2 cut(s) 59, 1150
BseMII CTCAG 5 cut(s) 245, 260, 384, 495, 869
BseNI ACTGG 3 cut(s) 102, 963, 1384
BseXI GCAGC 3 cut(s) 190, 907, 1003
BsgI GTGCAG 1 cut(s) 718
BshFI GGCC 1 cut(s) 665
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 86
BsiSI CCGG 1 cut(s) 1345
BslFI GGGAC 1 cut(s) 1006
BslI CCNNNNNNNGG 5 cut(s) 168, 232, 476, 699, 759
BsmAI GTCTC 2 cut(s) 460, 1151
BsmFI GGGAC 1 cut(s) 1006
BsnI GGCC 1 cut(s) 665
Bso31I GGTCTC 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 86, 482
BspACI CCGC 1 cut(s) 420
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 5 cut(s) 246, 259, 385, 494, 868
BspLI GGNNCC 4 cut(s) 7, 102, 186, 1404
BspMAI CTGCAG 1 cut(s) 208
BspQI GCTCTTC 1 cut(s) 1223
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 460
BsrDI GCAATG 2 cut(s) 59, 1150
BsrFI RCCGGY 1 cut(s) 1344
BsrI ACTGG 3 cut(s) 102, 963, 1384
BssAI RCCGGY 1 cut(s) 1344
BssECI CCNNGG 1 cut(s) 9
Bst2UI CCWGG 2 cut(s) 10, 1013
Bst4CI ACNGT 5 cut(s) 1020, 1056, 1089, 1299, 1341
Bst6I CTCTTC 2 cut(s) 993, 1223
BstC8I GCNNGC 3 cut(s) 223, 492, 912
BstDEI CTNAG 7 cut(s) 246, 254, 393, 481, 855, 885, 987
BstENI CCTNNNNNAGG 1 cut(s) 697
BstF5I GGATG 3 cut(s) 739, 817, 835
BstHHI GCGC 2 cut(s) 490, 1083
BstMAI GTCTC 2 cut(s) 460, 1151
BstMWI GCNNNNNNNGC 3 cut(s) 130, 832, 895
BstNI CCWGG 2 cut(s) 10, 1013
BstSCI CCNGG 2 cut(s) 8, 1011
BstSFI CTRYAG 3 cut(s) 189, 204, 890
BstV1I GCAGC 3 cut(s) 190, 907, 1003
BstV2I GAAGAC 3 cut(s) 248, 507, 989
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 1 cut(s) 665
BtsCI GGATG 3 cut(s) 739, 817, 835
BtsIMutI CAGTG 5 cut(s) 95, 561, 956, 1337, 1377
Cac8I GCNNGC 3 cut(s) 223, 492, 912
CaiI CAGNNNCTG 1 cut(s) 253
CfoI GCGC 2 cut(s) 490, 1083
Cfr10I RCCGGY 1 cut(s) 1344
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 3 cut(s) 6, 591, 725
CviAII CATG 2 cut(s) 367, 995
CviQI GTAC 3 cut(s) 6, 591, 725
DdeI CTNAG 7 cut(s) 246, 254, 393, 481, 855, 885, 987
DraI TTTAAA 2 cut(s) 598, 1165
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 993, 1223
EarI CTCTTC 2 cut(s) 993, 1223
Ecl136II GAGCTC 1 cut(s) 84
Eco24I GRGCYC 2 cut(s) 86, 482
Eco31I GGTCTC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 69, 492
EcoICRI GAGCTC 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 697
EcoRII CCWGG 2 cut(s) 8, 1011
EcoT38I GRGCYC 2 cut(s) 86, 482
FaeI CATG 2 cut(s) 370, 998
FaiI YATR 8 cut(s) 345, 368, 617, 933, 996, 1036, 1038, 1262
FalI AAGNNNNNCTT 6 cut(s) 578, 610, 1202, 1234, 1314, 1346
FaqI GGGAC 1 cut(s) 1006
FatI CATG 2 cut(s) 366, 994
FblI GTMKAC 1 cut(s) 1272
Fnu4HI GCNGC 3 cut(s) 204, 896, 992
FokI GGATG 3 cut(s) 746, 824, 842
FriOI GRGCYC 2 cut(s) 86, 482
Fsp4HI GCNGC 3 cut(s) 204, 896, 992
GlaI GCGC 2 cut(s) 489, 1082
GluI GCNGC 3 cut(s) 204, 896, 992
GsuI CTGGAG 3 cut(s) 87, 980, 995
HaeIII GGCC 1 cut(s) 665
HapII CCGG 1 cut(s) 1345
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 2 cut(s) 490, 1083
Hin1II CATG 2 cut(s) 370, 998
Hin6I GCGC 2 cut(s) 488, 1081
HinP1I GCGC 2 cut(s) 488, 1081
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HindIII AAGCTT 2 cut(s) 683, 1328
HinfI GANTC 9 cut(s) 56, 244, 308, 374, 557, 659, 689, 1334, 1397
HpaII CCGG 1 cut(s) 1345
HphI GGTGA 3 cut(s) 25, 206, 434
Hpy166II GTNNAC 5 cut(s) 624, 753, 1092, 1273, 1283
Hpy188III TCNNGA 7 cut(s) 47, 677, 1124, 1156, 1207, 1225, 1410
Hpy8I GTNNAC 5 cut(s) 624, 753, 1092, 1273, 1283
HpyAV CCTTC 6 cut(s) 127, 347, 410, 467, 499, 1204
HpyCH4III ACNGT 5 cut(s) 1020, 1056, 1089, 1299, 1341
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 5 cut(s) 206, 699, 741, 803, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 832, 895
HpyF3I CTNAG 7 cut(s) 246, 254, 393, 481, 855, 885, 987
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 2 cut(s) 370, 998
HspAI GCGC 2 cut(s) 488, 1081
KpnI GGTACC 1 cut(s) 9
LguI GCTCTTC 1 cut(s) 1223
LmnI GCTCC 5 cut(s) 89, 106, 340, 961, 1383
Lsp1109I GCAGC 3 cut(s) 190, 907, 1003
LweI GCATC 2 cut(s) 475, 790
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 6 cut(s) 140, 705, 1050, 1056, 1252, 1347
MboII GAAGA 8 cut(s) 253, 362, 507, 854, 994, 1010, 1240, 1303
MhlI GDGCHC 2 cut(s) 86, 482
MluCI AATT 8 cut(s) 157, 197, 499, 969, 1004, 1031, 1075, 1118
MlyI GAGTC 2 cut(s) 238, 698
MmeI TCCRAC 3 cut(s) 137, 1138, 1281
MnlI CCTC 4 cut(s) 343, 388, 418, 751
MseI TTAA 4 cut(s) 597, 878, 944, 1164
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 1 cut(s) 1345
MspR9I CCNGG 2 cut(s) 10, 1013
MvaI CCWGG 2 cut(s) 10, 1013
MwoI GCNNNNNNNGC 3 cut(s) 130, 832, 895
NlaIII CATG 2 cut(s) 370, 998
NlaIV GGNNCC 4 cut(s) 7, 102, 186, 1404
NmuCI GTSAC 3 cut(s) 1050, 1252, 1347
PciSI GCTCTTC 1 cut(s) 1223
PfeI GAWTC 7 cut(s) 56, 308, 374, 557, 659, 1334, 1397
PfoI TCCNGGA 1 cut(s) 1011
PkrI GCNGC 3 cut(s) 205, 897, 993
PleI GAGTC 2 cut(s) 238, 697
PpsI GAGTC 2 cut(s) 238, 697
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 8, 1011
PspGI CCWGG 2 cut(s) 8, 1011
PspN4I GGNNCC 4 cut(s) 7, 102, 186, 1404
PsrI GAACNNNNNNTAC 2 cut(s) 297, 329
PstI CTGCAG 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 3 cut(s) 7, 592, 726
RsaNI GTAC 3 cut(s) 6, 591, 725
SacI GAGCTC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 1223
SaqAI TTAA 4 cut(s) 597, 878, 944, 1164
SatI GCNGC 3 cut(s) 204, 896, 992
ScaI AGTACT 1 cut(s) 726
SchI GAGTC 2 cut(s) 238, 698
ScrFI CCNGG 2 cut(s) 10, 1013
SduI GDGCHC 2 cut(s) 86, 482
SfaNI GCATC 2 cut(s) 475, 790
SfcI CTRYAG 3 cut(s) 189, 204, 890
SmlI CTYRAG 3 cut(s) 686, 769, 1205
SmoI CTYRAG 3 cut(s) 686, 769, 1205
Sse9I AATT 8 cut(s) 157, 197, 499, 969, 1004, 1031, 1075, 1118
SsiI CCGC 1 cut(s) 420
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 2 cut(s) 8, 1011
TaaI ACNGT 5 cut(s) 1020, 1056, 1089, 1299, 1341
TaiI ACGT 1 cut(s) 577
TaqI TCGA 3 cut(s) 120, 676, 1155
TasI AATT 8 cut(s) 157, 197, 499, 969, 1004, 1031, 1075, 1118
TatI WGTACW 1 cut(s) 724
TfiI GAWTC 7 cut(s) 56, 308, 374, 557, 659, 1334, 1397
Tru1I TTAA 4 cut(s) 597, 878, 944, 1164
Tru9I TTAA 4 cut(s) 597, 878, 944, 1164
TscAI CASTG 5 cut(s) 102, 568, 963, 1344, 1384
TseFI GTSAC 3 cut(s) 1050, 1252, 1347
TseI GCWGC 3 cut(s) 203, 895, 991
Tsp45I GTSAC 3 cut(s) 1050, 1252, 1347
TspDTI ATGAA 9 cut(s) 48, 348, 507, 604, 885, 948, 1011, 1043, 1161
TspGWI ACGGA 2 cut(s) 261, 353
TspRI CASTG 5 cut(s) 102, 568, 963, 1344, 1384
XagI CCTNNNNNAGG 1 cut(s) 697
XapI RAATTY 1 cut(s) 1075
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XmiI GTMKAC 1 cut(s) 1272
ZrmI AGTACT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.