MD01G1112500.v1.1

Amino-transferase class IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004670
Physical Location & Seq
Reverse (-)
22663693 .. 22675772
12080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1112500.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGCGCGTTTCTTGTTCACCAACGGCGTTGTTTCGCCACCCGCCGCCACTCCTCCGGTCGCCGCCTTCCTCGAAACCCACCCAGGCGCGTACACAACGTCCCGAACTCACAACGACGCGTCGTTCGTGCTGTTCTGGGAAAGGCACCTGAGGAGACTGGCGGACTCTGTGACAATCCTCTTCAATTCGAATCCTCAACTTCTCTTCGGACCCCACTGCAAAACGACGCCGCCTTCGCTGCCGTCTCTGTCGTCCGACTCCATCTCTCTCCGATCGACGATTCGCGAGCTCGTGAACCGCTCGATGAAGCAAGTGCTGCCGATTGCTATGGAGGAGAGGAGGGAGGGGGAGGAGCTGTCGATTACGGCGTTGGTCAGTGGCAATTTGGAGAAATTGAGGGTTGGGGATGAGGGTTTTGATGTGAGTATCTACATTGGTAGTTATGTGCCTGCTGTGTTTGGTATCAGAGAAAACGGTGCGTTTTTGGCTGTGGTGGGTCGCGGGAGAGATTTTGCTGCTGCAAAGTACTCGGATTGGGTAAGGATGAGGAAGTCTTTGGAGAGGTTGAGGCCGCCGAAGGGGACGGAGCTCTTGTTGTCCAATGATGGTGATCGGATTCTTGAGGGCACCGTGTCTAACTTGTTCGTTGTCCGCCGGAAGGATATCAGTGAAGCTAAAGCCGAAAGTATGCACTGTTTTGAAGTACAGACAGCCCCTCTTAGTGGTGTCCTTCCAGGAATTATTCGGCAACTGGTCATTGAAGTCTGCTTAAGCAAGGGAATTCCCTTGAGAGAAGTTGCACCATTGTGGTCAGAGAGAGAATTTTGGGCAGAGGCATTCATTACAAGCAGCTTGAGACTTCTGCAGCACGCGAAGACAATTAGTTTTCCAAGTTCATGGGAATCACTGGATTCTAATTCTTTGGAGGACATCACATGGATAGACAAGCATTTTGAGGACGGCCCTGGGATGATCACGACAATAATCCAGAAAGAGGTCATGGAAAAGGCAGTTTTAGAAGGGTACTCCGTCAGTTACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

348

Amino Acids

38.67

Weight (kDa)

6.04

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 29 - 290 5.4e-13 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1042
AccB1I GGYRCC 2 cut(s) 144, 626
AccBSI CCGCTC 1 cut(s) 300
AccII CGCG 6 cut(s) 7, 89, 119, 285, 501, 872
AciI CCGC 9 cut(s) 42, 45, 63, 161, 230, 298, 501, 572, 652
AcsI RAATTY 2 cut(s) 780, 821
AcyI GRCGYC 1 cut(s) 227
AfaI GTAC 4 cut(s) 92, 527, 705, 1025
AfiI CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
AflII CTTAAG 1 cut(s) 769
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 3 cut(s) 184, 701, 761
AjnI CCWGG 3 cut(s) 82, 733, 964
AleI CACNNNNGTG 1 cut(s) 805
AluBI AGCT 5 cut(s) 289, 355, 589, 674, 852
AluI AGCT 5 cut(s) 289, 355, 589, 674, 852
Alw21I GWGCWC 2 cut(s) 291, 591
Alw26I GTCTC 3 cut(s) 148, 249, 850
AoxI GGCC 2 cut(s) 569, 961
ApeKI GCWGC 6 cut(s) 238, 316, 515, 518, 849, 865
ApoI RAATTY 2 cut(s) 780, 821
ArsI GACNNNNNNTTYG 6 cut(s) 107, 139, 217, 249, 935, 967
AspLEI GCGC 2 cut(s) 7, 89
AspS9I GGNCC 2 cut(s) 209, 962
AsuHPI GGTGA 2 cut(s) 10, 620
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 629
BanI GGYRCC 2 cut(s) 144, 626
BanII GRGCYC 2 cut(s) 291, 591
BauI CACGAG 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 881
Bbv12I GWGCWC 2 cut(s) 291, 591
BbvI GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
BccI CCATC 2 cut(s) 269, 599
BceAI ACGGC 4 cut(s) 40, 226, 381, 976
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 3 cut(s) 84, 735, 966
BclI TGATCA 1 cut(s) 972
BcoDI GTCTC 3 cut(s) 148, 249, 850
BfmI CTRYAG 1 cut(s) 863
BfrI CTTAAG 1 cut(s) 769
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 3 cut(s) 84, 735, 966
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 209, 962
BmiI GGNNCC 3 cut(s) 146, 211, 628
BmrFI CCNGG 3 cut(s) 84, 735, 966
BpiI GAAGAC 1 cut(s) 881
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 3 cut(s) 641, 808, 874
BsaBI GATNNNNATC 1 cut(s) 609
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 82, 964, 965
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 4 cut(s) 341, 371, 578, 608
Bsc4I CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
Bse1I ACTGG 3 cut(s) 162, 756, 912
Bse21I CCTNAGG 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 3 cut(s) 84, 735, 966
BseDI CCNNGG 3 cut(s) 82, 964, 965
BseGI GGATG 3 cut(s) 412, 549, 975
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 3 cut(s) 162, 756, 912
BseRI GAGGAG 5 cut(s) 42, 166, 347, 352, 365
BseSI GKGCMC 1 cut(s) 629
BseXI GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
Bsh1236I CGCG 6 cut(s) 7, 89, 119, 285, 501, 872
Bsh1285I CGRYCG 2 cut(s) 60, 275
BshFI GGCC 2 cut(s) 571, 963
BshNI GGYRCC 2 cut(s) 144, 626
BsiEI CGRYCG 2 cut(s) 60, 275
BsiHKAI GWGCWC 2 cut(s) 291, 591
BsiSI CCGG 2 cut(s) 56, 655
BslFI GGGAC 2 cut(s) 85, 595
BslI CCNNNNNNNGG 4 cut(s) 578, 658, 722, 994
BsmAI GTCTC 3 cut(s) 148, 249, 850
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 2 cut(s) 85, 595
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 2 cut(s) 571, 963
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 291, 591, 629
Bsp143I GATC 3 cut(s) 272, 610, 972
Bsp68I TCGCGA 1 cut(s) 285
BspACI CCGC 9 cut(s) 42, 45, 63, 161, 230, 298, 501, 572, 652
BspANI GGCC 2 cut(s) 571, 963
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 6 cut(s) 7, 89, 119, 285, 501, 872
BspLI GGNNCC 3 cut(s) 146, 211, 628
BspMAI CTGCAG 1 cut(s) 867
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 2 cut(s) 144, 626
BspTI CTTAAG 1 cut(s) 769
BsrBI CCGCTC 1 cut(s) 300
BsrI ACTGG 3 cut(s) 162, 756, 912
BssECI CCNNGG 3 cut(s) 82, 964, 965
BssMI GATC 3 cut(s) 272, 610, 972
BssNI GRCGYC 1 cut(s) 227
BssSI CACGAG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 290
Bst2UI CCWGG 3 cut(s) 84, 735, 966
Bst4CI ACNGT 3 cut(s) 476, 631, 695
Bst6I CTCTTC 2 cut(s) 185, 209
BstACI GRCGYC 1 cut(s) 227
BstAFI CTTAAG 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 3 cut(s) 287, 450, 870
BstDEI CTNAG 2 cut(s) 149, 719
BstF5I GGATG 3 cut(s) 412, 549, 975
BstFNI CGCG 6 cut(s) 7, 89, 119, 285, 501, 872
BstHHI GCGC 2 cut(s) 7, 89
BstKTI GATC 3 cut(s) 275, 613, 975
BstMAI GTCTC 3 cut(s) 148, 249, 850
BstMBI GATC 3 cut(s) 272, 610, 972
BstMCI CGRYCG 2 cut(s) 60, 275
BstMWI GCNNNNNNNGC 4 cut(s) 235, 238, 316, 485
BstNI CCWGG 3 cut(s) 84, 735, 966
BstSCI CCNGG 3 cut(s) 82, 733, 964
BstSFI CTRYAG 1 cut(s) 863
BstSLI GKGCMC 1 cut(s) 629
BstUI CGCG 6 cut(s) 7, 89, 119, 285, 501, 872
BstV1I GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
BstV2I GAAGAC 1 cut(s) 881
BstXI CCANNNNNNTGG 1 cut(s) 897
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 2 cut(s) 571, 963
BtsCI GGATG 3 cut(s) 412, 549, 975
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 5 cut(s) 214, 382, 673, 691, 905
BtuMI TCGCGA 1 cut(s) 285
Cac8I GCNNGC 3 cut(s) 287, 450, 870
CfoI GCGC 2 cut(s) 7, 89
Cfr13I GGNCC 2 cut(s) 209, 962
CseI GACGC 3 cut(s) 108, 125, 235
Csp6I GTAC 4 cut(s) 91, 526, 704, 1024
CviAII CATG 3 cut(s) 897, 936, 1000
CviQI GTAC 4 cut(s) 91, 526, 704, 1024
DdeI CTNAG 2 cut(s) 149, 719
DpnI GATC 3 cut(s) 274, 612, 974
DpnII GATC 3 cut(s) 272, 610, 972
Eam1104I CTCTTC 2 cut(s) 185, 209
EarI CTCTTC 2 cut(s) 185, 209
EciI GGCGGA 2 cut(s) 176, 641
Ecl136II GAGCTC 2 cut(s) 289, 589
Eco24I GRGCYC 2 cut(s) 291, 591
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 2 cut(s) 289, 589
Eco81I CCTNAGG 1 cut(s) 149
EcoICRI GAGCTC 2 cut(s) 289, 589
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 3 cut(s) 82, 733, 964
EcoRV GATATC 1 cut(s) 664
EcoT38I GRGCYC 2 cut(s) 291, 591
Esp3I CGTCTC 1 cut(s) 249
FaeI CATG 3 cut(s) 900, 939, 1003
FaiI YATR 7 cut(s) 329, 444, 689, 898, 937, 1001, 1042
FaqI GGGAC 2 cut(s) 85, 595
FatI CATG 3 cut(s) 896, 935, 999
FauI CCCGC 2 cut(s) 49, 494
FbaI TGATCA 1 cut(s) 972
FokI GGATG 3 cut(s) 419, 556, 982
FriOI GRGCYC 2 cut(s) 291, 591
GlaI GCGC 2 cut(s) 6, 88
HaeIII GGCC 2 cut(s) 571, 963
HapII CCGG 2 cut(s) 56, 655
HgaI GACGC 3 cut(s) 108, 125, 235
HhaI GCGC 2 cut(s) 7, 89
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 3 cut(s) 900, 939, 1003
Hin6I GCGC 2 cut(s) 5, 87
HinP1I GCGC 2 cut(s) 5, 87
HinfI GANTC 7 cut(s) 164, 190, 257, 280, 616, 902, 911
HpaII CCGG 2 cut(s) 56, 655
HphI GGTGA 2 cut(s) 10, 620
Hpy166II GTNNAC 3 cut(s) 18, 93, 295
Hpy188I TCNGA 7 cut(s) 209, 256, 272, 467, 532, 615, 814
Hpy188III TCNNGA 6 cut(s) 102, 284, 292, 620, 976, 988
Hpy8I GTNNAC 3 cut(s) 18, 93, 295
Hpy99I CGWCG 4 cut(s) 119, 124, 229, 280
HpyAV CCTTC 6 cut(s) 76, 243, 571, 652, 740, 1013
HpyCH4III ACNGT 3 cut(s) 476, 631, 695
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 5 cut(s) 219, 521, 691, 800, 865
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 238, 316, 485
HpyF3I CTNAG 2 cut(s) 149, 719
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 3 cut(s) 900, 939, 1003
HspAI GCGC 2 cut(s) 5, 87
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 3 cut(s) 272, 610, 972
LmnI GCTCC 2 cut(s) 352, 586
Lsp1109I GCAGC 6 cut(s) 225, 303, 502, 505, 861, 877
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 169, 1034
MalI GATC 3 cut(s) 274, 612, 974
MbiI CCGCTC 1 cut(s) 300
MboI GATC 3 cut(s) 272, 610, 972
MboII GAAGA 3 cut(s) 172, 196, 886
MhlI GDGCHC 3 cut(s) 291, 591, 629
MluCI AATT 8 cut(s) 184, 382, 392, 738, 780, 821, 879, 916
MluI ACGCGT 1 cut(s) 117
MlyI GAGTC 2 cut(s) 158, 251
MmeI TCCRAC 1 cut(s) 279
MseI TTAA 1 cut(s) 770
MslI CAYNNNNRTG 1 cut(s) 805
MspCI CTTAAG 1 cut(s) 769
MspI CCGG 2 cut(s) 56, 655
MspR9I CCNGG 3 cut(s) 84, 735, 966
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 3 cut(s) 84, 735, 966
MvnI CGCG 6 cut(s) 7, 89, 119, 285, 501, 872
MwoI GCNNNNNNNGC 4 cut(s) 235, 238, 316, 485
NdeII GATC 3 cut(s) 272, 610, 972
NlaIII CATG 3 cut(s) 900, 939, 1003
NlaIV GGNNCC 3 cut(s) 146, 211, 628
NmuCI GTSAC 1 cut(s) 169
NruI TCGCGA 1 cut(s) 285
NspV TTCGAA 1 cut(s) 188
OliI CACNNNNGTG 1 cut(s) 805
PasI CCCWGGG 1 cut(s) 965
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 365
PctI GAATGC 1 cut(s) 836
PfeI GAWTC 5 cut(s) 190, 280, 616, 902, 911
PfoI TCCNGGA 1 cut(s) 733
Ple19I CGATCG 1 cut(s) 275
PleI GAGTC 2 cut(s) 158, 251
PpsI GAGTC 2 cut(s) 158, 251
PsiI TTATAA 1 cut(s) 1042
Psp124BI GAGCTC 2 cut(s) 291, 591
Psp6I CCWGG 3 cut(s) 82, 733, 964
PspGI CCWGG 3 cut(s) 82, 733, 964
PspN4I GGNNCC 3 cut(s) 146, 211, 628
PspPI GGNCC 2 cut(s) 209, 962
PstI CTGCAG 1 cut(s) 867
PvuI CGATCG 1 cut(s) 275
RruI TCGCGA 1 cut(s) 285
RsaI GTAC 4 cut(s) 92, 527, 705, 1025
RsaNI GTAC 4 cut(s) 91, 526, 704, 1024
RseI CAYNNNNRTG 1 cut(s) 805
SacI GAGCTC 2 cut(s) 291, 591
SaqAI TTAA 1 cut(s) 770
Sau3AI GATC 3 cut(s) 272, 610, 972
Sau96I GGNCC 2 cut(s) 209, 962
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 158, 251
ScrFI CCNGG 3 cut(s) 84, 735, 966
SduI GDGCHC 3 cut(s) 291, 591, 629
SetI ASST 9 cut(s) 101, 150, 291, 357, 566, 591, 676, 854, 999
SfcI CTRYAG 1 cut(s) 863
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 805
SmlI CTYRAG 4 cut(s) 620, 769, 787, 853
SmoI CTYRAG 4 cut(s) 620, 769, 787, 853
Sse9I AATT 8 cut(s) 184, 382, 392, 738, 780, 821, 879, 916
SsiI CCGC 9 cut(s) 42, 45, 63, 161, 230, 298, 501, 572, 652
SstI GAGCTC 2 cut(s) 291, 591
StyD4I CCNGG 3 cut(s) 82, 733, 964
TaaI ACNGT 3 cut(s) 476, 631, 695
TaiI ACGT 1 cut(s) 101
TaqI TCGA 5 cut(s) 72, 188, 275, 302, 359
TasI AATT 8 cut(s) 184, 382, 392, 738, 780, 821, 879, 916
TatI WGTACW 2 cut(s) 525, 703
TauI GCSGC 4 cut(s) 47, 65, 232, 574
TfiI GAWTC 5 cut(s) 190, 280, 616, 902, 911
Tru1I TTAA 1 cut(s) 770
Tru9I TTAA 1 cut(s) 770
TscAI CASTG 5 cut(s) 221, 382, 673, 698, 912
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 6 cut(s) 238, 316, 515, 518, 849, 865
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 320, 829, 885
TspGWI ACGGA 2 cut(s) 599, 1018
TspRI CASTG 5 cut(s) 221, 382, 673, 698, 912
Vha464I CTTAAG 1 cut(s) 769
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 2 cut(s) 780, 821
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.