RchiOBHm_Chr1g0364231

Amino-transferase class IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004670
Physical Location & Seq
Reverse (-)
55474237 .. 55477883
3647 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58892

Sequence Viewer

Length: 1044 bp
ATGACGACTACTCCTCTCAGGTTCTTGCTCACCAACGGCGTCGTCTCCAACCCTGCCAACACTCCTCCCGTCACCACCTTCCTCGAGACCCACCCAGGGGCTTACACGACGACTCGCACTCACAACAACGCTTCATTCGTGTTGTTTTGGGATAGGCACTTGAAAAGGCTCGCGGAGTCCGTGAGAATCCTGTACAATGCCGATCCTCAGCTTCTGTTCGGACCCAACAAGACTTCGCTTCCATTACCGTCGTCTCTTGCAGCCAATTCTCTTTTGCTCCACGATCTCGTGAATGATTCGATGAAAAAAGTGCTGCCAATTGCTTTGGAGGGGAGGAGTGACGGGGAGGAACTGTCGATTACTACTTTGGTCAGTGGCAATTTGGGGAAATTGGAGCTAGAAAAAATTGGCGGGGCTCTTGATGTGAGCATTCACGTTGGGATTTATGTGCCTTCTGTGTTTGGTGTCAGAGGAAGCGGTGCGCAGTTGGCTGTGGTGGGTCGTGGGAGAGATGTTGCTGCTGCTAAGTACTCGGATTGGGTGAGGATTAGGAAGTCTTTGGAGAGGCTGAGGCCGCCCTCAGGGACCGAGCTCTTGTTGTCCAATGATGGGGATCGGATTCTTGAAGGCAGCCTGTCAAACTTCTTTGTGGTCCGCCGAAAGGATGACAATGAAGCTAAAGAGCAAACTATCCACTGTTTTGAAGTACAGACAGCTCCTATTAGTGAGGGTGTCCTACCAGGAATTATACGCCAACTAGTCATTGAAGTATGCTTGAGCAAAGGAATCCCTTTTCGAGAAGTTGCACCACTGTGGTCAGAGCATGAATTTTGGGAAGAGGCGTTCATTACAAATAGCTTAAGACTTATGCAGCATGCGGAGATTGTTCGTGCTCCTAGTTCATGGGCATCACTGCATGGTAATTCATGGAAGGACATATCTTGGAAAGAGAAGCAGTTTGAGGAGGGTCCTGGAATGATTACTGCAATACTCCAGAAGGAGATCATGGAAAAAGCAGTTTCAGAAGGATACTCTTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

347

Amino Acids

38.35

Weight (kDa)

6.17

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 197 - 291 1.2e-10 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 483
AccII CGCG 1 cut(s) 173
AciI CCGC 6 cut(s) 173, 411, 477, 575, 655, 878
AclWI GGATC 2 cut(s) 197, 621
AcsI RAATTY 1 cut(s) 827
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 194, 530, 708
AfiI CCNNNNNNNGG 5 cut(s) 96, 97, 581, 609, 661
AflII CTTAAG 1 cut(s) 859
AgsI TTSAA 4 cut(s) 163, 626, 704, 767
AhlI ACTAGT 1 cut(s) 757
AjnI CCWGG 3 cut(s) 94, 739, 970
AleI CACNNNNGTG 1 cut(s) 811
AloI GAACNNNNNNTCC 2 cut(s) 827, 859
AluBI AGCT 6 cut(s) 211, 397, 592, 677, 716, 858
AluI AGCT 6 cut(s) 211, 397, 592, 677, 716, 858
Alw21I GWGCWC 2 cut(s) 594, 895
Alw26I GTCTC 3 cut(s) 49, 80, 258
AlwI GGATC 2 cut(s) 197, 621
AlwNI CAGNNNCTG 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 572
ApeKI GCWGC 6 cut(s) 260, 313, 518, 521, 630, 871
ApoI RAATTY 1 cut(s) 827
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 4 cut(s) 221, 585, 652, 968
AsuHPI GGTGA 3 cut(s) 22, 64, 553
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 4 cut(s) 221, 585, 652, 968
AxyI CCTNAGG 1 cut(s) 580
BanII GRGCYC 2 cut(s) 418, 594
BauI CACGAG 1 cut(s) 287
Bbv12I GWGCWC 2 cut(s) 594, 895
BbvCI CCTCAGC 2 cut(s) 207, 569
BbvI GCAGC 6 cut(s) 272, 300, 505, 508, 642, 883
BccI CCATC 1 cut(s) 602
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 3 cut(s) 96, 741, 972
BciVI GTATCC 1 cut(s) 1022
BcoDI GTCTC 3 cut(s) 49, 80, 258
BcuI ACTAGT 1 cut(s) 757
BfaI CTAG 3 cut(s) 398, 758, 897
BfrI CTTAAG 1 cut(s) 859
BfuI GTATCC 1 cut(s) 1022
BisI GCNGC 7 cut(s) 261, 314, 519, 522, 575, 631, 872
BlsI GCNGC 7 cut(s) 262, 315, 520, 523, 576, 632, 873
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 3 cut(s) 96, 741, 972
Bme18I GGWCC 4 cut(s) 221, 585, 652, 968
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 221, 585, 652, 968
BmiI GGNNCC 3 cut(s) 223, 586, 969
BmrFI CCNGG 3 cut(s) 96, 741, 972
BmsI GCATC 1 cut(s) 917
BpmI CTGGAG 1 cut(s) 977
Bpu10I CCTNAGC 2 cut(s) 207, 569
BpuEI CTTGAG 1 cut(s) 796
BsaBI GATNNNNATC 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 94, 95
BsaXI ACNNNNNCTCC 3 cut(s) 25, 338, 368
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 97, 581, 609, 661
Bse21I CCTNAGG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 612
BseBI CCWGG 3 cut(s) 96, 741, 972
BseDI CCNNGG 2 cut(s) 94, 95
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 5 cut(s) 96, 97, 581, 609, 661
BseMII CTCAG 4 cut(s) 31, 221, 560, 594
BseRI GAGGAG 3 cut(s) 54, 349, 977
BseXI GCAGC 6 cut(s) 272, 300, 505, 508, 642, 883
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 574
BsiHKAI GWGCWC 2 cut(s) 594, 895
BsiHKCI CYCGRG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 5 cut(s) 96, 97, 581, 609, 661
BsmAI GTCTC 3 cut(s) 49, 80, 258
BsmBI CGTCTC 2 cut(s) 49, 258
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 1 cut(s) 574
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 3 cut(s) 418, 594, 895
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 4 cut(s) 202, 283, 613, 1002
BspACI CCGC 6 cut(s) 173, 411, 477, 575, 655, 878
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 4 cut(s) 30, 220, 561, 593
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 3 cut(s) 223, 586, 969
BspPI GGATC 2 cut(s) 197, 621
BspTI CTTAAG 1 cut(s) 859
BspTNI GGTCTC 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 192
BssECI CCNNGG 2 cut(s) 94, 95
BssMI GATC 4 cut(s) 202, 283, 613, 1002
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst2UI CCWGG 3 cut(s) 96, 741, 972
Bst4CI ACNGT 4 cut(s) 249, 354, 698, 813
Bst6I CTCTTC 1 cut(s) 831
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 859
BstAUI TGTACA 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 171, 876
BstDEI CTNAG 5 cut(s) 17, 207, 525, 569, 580
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 4 cut(s) 205, 286, 616, 1005
BstMAI GTCTC 3 cut(s) 49, 80, 258
BstMBI GATC 4 cut(s) 202, 283, 613, 1002
BstMWI GCNNNNNNNGC 2 cut(s) 488, 574
BstNI CCWGG 3 cut(s) 96, 741, 972
BstNSI RCATGY 1 cut(s) 878
BstSCI CCNGG 3 cut(s) 94, 739, 970
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 6 cut(s) 272, 300, 505, 508, 642, 883
Bsu36I CCTNAGG 1 cut(s) 580
BsuI GTATCC 1 cut(s) 1022
BsuRI GGCC 1 cut(s) 574
BtsCI GGATG 1 cut(s) 670
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 4 cut(s) 379, 694, 809, 911
Cac8I GCNNGC 2 cut(s) 171, 876
CaiI CAGNNNCTG 1 cut(s) 214
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 4 cut(s) 221, 585, 652, 968
CseI GACGC 1 cut(s) 28
Csp6I GTAC 3 cut(s) 193, 529, 707
CviAII CATG 6 cut(s) 824, 875, 903, 917, 927, 1006
CviQI GTAC 3 cut(s) 193, 529, 707
DdeI CTNAG 5 cut(s) 17, 207, 525, 569, 580
DpnI GATC 4 cut(s) 204, 285, 615, 1004
DpnII GATC 4 cut(s) 202, 283, 613, 1002
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 1 cut(s) 644
Ecl136II GAGCTC 1 cut(s) 592
Eco24I GRGCYC 2 cut(s) 418, 594
Eco31I GGTCTC 1 cut(s) 80
Eco47I GGWCC 4 cut(s) 221, 585, 652, 968
Eco53kI GAGCTC 1 cut(s) 592
Eco81I CCTNAGG 1 cut(s) 580
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 592
EcoO109I RGGNCCY 1 cut(s) 968
EcoRII CCWGG 3 cut(s) 94, 739, 970
EcoT38I GRGCYC 2 cut(s) 418, 594
Esp3I CGTCTC 2 cut(s) 49, 258
FaeI CATG 6 cut(s) 827, 878, 906, 920, 930, 1009
FaqI GGGAC 1 cut(s) 598
FatI CATG 6 cut(s) 823, 874, 902, 916, 926, 1005
FauI CCCGC 1 cut(s) 404
Fnu4HI GCNGC 7 cut(s) 261, 314, 519, 522, 575, 631, 872
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 2 cut(s) 418, 594
Fsp4HI GCNGC 7 cut(s) 261, 314, 519, 522, 575, 631, 872
FspBI CTAG 3 cut(s) 398, 758, 897
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 1 cut(s) 483
GluI GCNGC 7 cut(s) 261, 314, 519, 522, 575, 631, 872
GsuI CTGGAG 1 cut(s) 977
HaeIII GGCC 1 cut(s) 574
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 484
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 827, 878, 906, 920, 930, 1009
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HinfI GANTC 6 cut(s) 112, 176, 186, 296, 619, 786
HphI GGTGA 3 cut(s) 22, 64, 553
Hpy188I TCNGA 6 cut(s) 221, 470, 535, 618, 820, 1024
Hpy188III TCNNGA 6 cut(s) 85, 289, 419, 623, 797, 994
Hpy99I CGWCG 3 cut(s) 44, 112, 253
HpyAV CCTTC 6 cut(s) 88, 462, 620, 925, 991, 1019
HpyCH4III ACNGT 4 cut(s) 249, 354, 698, 813
HpyCH4IV ACGT 1 cut(s) 435
HpyCH4V TGCA 5 cut(s) 260, 806, 871, 916, 986
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 574
HpyF3I CTNAG 5 cut(s) 17, 207, 525, 569, 580
HpySE526I ACGT 1 cut(s) 435
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 827, 878, 906, 920, 930, 1009
HspAI GCGC 1 cut(s) 482
Kzo9I GATC 4 cut(s) 202, 283, 613, 1002
LmnI GCTCC 4 cut(s) 282, 394, 721, 898
Lsp1109I GCAGC 6 cut(s) 272, 300, 505, 508, 642, 883
LweI GCATC 1 cut(s) 917
MaeI CTAG 3 cut(s) 398, 758, 897
MaeII ACGT 1 cut(s) 435
MaeIII GTNAC 2 cut(s) 70, 338
MalI GATC 4 cut(s) 204, 285, 615, 1004
MboI GATC 4 cut(s) 202, 283, 613, 1002
MboII GAAGA 1 cut(s) 848
MfeI CAATTG 1 cut(s) 318
MhlI GDGCHC 3 cut(s) 418, 594, 895
MluCI AATT 8 cut(s) 265, 318, 379, 389, 405, 744, 827, 922
MlyI GAGTC 2 cut(s) 106, 185
MmeI TCCRAC 1 cut(s) 72
MseI TTAA 1 cut(s) 860
MslI CAYNNNNRTG 1 cut(s) 811
MspCI CTTAAG 1 cut(s) 859
MspR9I CCNGG 3 cut(s) 96, 741, 972
MunI CAATTG 1 cut(s) 318
Mva1269I GAATGC 1 cut(s) 429
MvaI CCWGG 3 cut(s) 96, 741, 972
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 488, 574
NdeII GATC 4 cut(s) 202, 283, 613, 1002
NlaIII CATG 6 cut(s) 827, 878, 906, 920, 930, 1009
NlaIV GGNNCC 3 cut(s) 223, 586, 969
NmuCI GTSAC 2 cut(s) 70, 338
NsbI TGCGCA 1 cut(s) 483
NspI RCATGY 1 cut(s) 878
OliI CACNNNNGTG 1 cut(s) 811
PaeI GCATGC 1 cut(s) 878
PaeR7I CTCGAG 1 cut(s) 83
PasI CCCWGGG 1 cut(s) 95
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 177
PctI GAATGC 1 cut(s) 429
PfeI GAWTC 4 cut(s) 186, 296, 619, 786
PfoI TCCNGGA 1 cut(s) 970
PkrI GCNGC 7 cut(s) 262, 315, 520, 523, 576, 632, 873
PleI GAGTC 2 cut(s) 106, 184
PpsI GAGTC 2 cut(s) 106, 184
PpuMI RGGWCCY 1 cut(s) 968
Psp124BI GAGCTC 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 968
Psp6I CCWGG 3 cut(s) 94, 739, 970
PspGI CCWGG 3 cut(s) 94, 739, 970
PspN4I GGNNCC 3 cut(s) 223, 586, 969
PspPI GGNCC 4 cut(s) 221, 585, 652, 968
PspPPI RGGWCCY 1 cut(s) 968
PstNI CAGNNNCTG 1 cut(s) 214
RsaI GTAC 3 cut(s) 194, 530, 708
RsaNI GTAC 3 cut(s) 193, 529, 707
RseI CAYNNNNRTG 1 cut(s) 811
SacI GAGCTC 1 cut(s) 594
SaqAI TTAA 1 cut(s) 860
SatI GCNGC 7 cut(s) 261, 314, 519, 522, 575, 631, 872
Sau3AI GATC 4 cut(s) 202, 283, 613, 1002
Sau96I GGNCC 4 cut(s) 221, 585, 652, 968
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 2 cut(s) 106, 185
ScrFI CCNGG 3 cut(s) 96, 741, 972
SduI GDGCHC 3 cut(s) 418, 594, 895
SetI ASST 9 cut(s) 23, 80, 213, 399, 438, 594, 679, 718, 860
SfaNI GCATC 1 cut(s) 917
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 4 cut(s) 221, 585, 652, 968
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 811
SmlI CTYRAG 3 cut(s) 83, 775, 859
SmoI CTYRAG 3 cut(s) 83, 775, 859
SpeI ACTAGT 1 cut(s) 757
SphI GCATGC 1 cut(s) 878
Sse9I AATT 8 cut(s) 265, 318, 379, 389, 405, 744, 827, 922
SsiI CCGC 6 cut(s) 173, 411, 477, 575, 655, 878
SspMI CTAG 3 cut(s) 398, 758, 897
SstI GAGCTC 1 cut(s) 594
StyD4I CCNGG 3 cut(s) 94, 739, 970
TaaI ACNGT 4 cut(s) 249, 354, 698, 813
TaiI ACGT 1 cut(s) 438
TaqI TCGA 4 cut(s) 84, 299, 356, 796
TaqII GACCGA 1 cut(s) 602
TasI AATT 8 cut(s) 265, 318, 379, 389, 405, 744, 827, 922
TatI WGTACW 3 cut(s) 192, 528, 706
TauI GCSGC 1 cut(s) 577
TfiI GAWTC 4 cut(s) 186, 296, 619, 786
Tru1I TTAA 1 cut(s) 860
Tru9I TTAA 1 cut(s) 860
TscAI CASTG 4 cut(s) 379, 701, 816, 918
TseFI GTSAC 2 cut(s) 70, 338
TseI GCWGC 6 cut(s) 260, 313, 518, 521, 630, 871
Tsp45I GTSAC 2 cut(s) 70, 338
TspDTI ATGAA 7 cut(s) 123, 317, 687, 835, 840, 891, 915
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 4 cut(s) 379, 701, 816, 918
Vha464I CTTAAG 1 cut(s) 859
VpaK11BI GGWCC 4 cut(s) 221, 585, 652, 968
XapI RAATTY 1 cut(s) 827
XceI RCATGY 1 cut(s) 878
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 3 cut(s) 398, 758, 897
ZrmI AGTACT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.