Rorug01G0315100

Amino-transferase class IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001240
Physical Location & Seq
Reverse (-)
43305569 .. 43308276
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0315100.1

Sequence Viewer

Length: 756 bp
ATGGGTCTGCTGAGTAACAAAGTTGAGAGGGATGAGATCAAGCCAGGAGATCACATCTACACTTACAGAGCTGTCTTCACCTACTCTCACCATGGTATTTATCTAGGAGGAAGCAAGGTGGTGCATTTTACACCGGAGAAAAAGCCTAGTTCAAGCACTGGCAAATCTTATGATTCAATGTCCTGTCCCACCTTTCCTGACTGTGGATTCAGGCAACCGAATAGTGGTGTTGTCCTTTCATGCCTCGATTGCTTCCTGAGAAATGGATCCATTTACTGCTTTGAATATGGGGTTACCCCTTCAATTTTCCTATCCAAAGTACGGGGTGGCACATGCACCACTGCAGCATCTGACTCCCCTGAATCTGTTATCCACCGTGCTATGTATCTTCTTCAGAATGGATTTGGCAATTACGACGTCTTTCAAAATAACTGTGAGGATTTTGCAATGTATTGCAAAACTGGTCTCCTGATAATGGATAAGCAGGGAGTCGGAAGAAGTGGCCAAGCTGCTTCCGTTATTGGTGCCCCACTGGCGGCCATGCTTTCTTCTCCTCTAACGTTGTTGATGCCAGGCCCAGTTGGTGTTGCTACCGCAACAGCCGGGATGTACTGTATAAGCAGGTATTCGACTGACATTGGTGTCCGAACTGATGTGATCAAGGTGGCCGTGGAGGATTTGGCTGTGAATTTGGGGTGGACAACTCACCATGAGGAAGAGGCCAAGGATAGTGAAGCTCCTTTGATACAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

251

Amino Acids

27.14

Weight (kDa)

5.96

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 156 2.5e-32 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AatII GACGTC 1 cut(s) 420
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 524
AciI CCGC 2 cut(s) 536, 594
AclI AACGTT 1 cut(s) 560
AclWI GGATC 2 cut(s) 261, 274
AcoI YGGCCR 3 cut(s) 502, 537, 666
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 2 cut(s) 321, 611
AfiI CCNNNNNNNGG 5 cut(s) 203, 224, 321, 475, 535
AgsI TTSAA 5 cut(s) 153, 177, 284, 303, 425
AjnI CCWGG 2 cut(s) 43, 571
AluBI AGCT 4 cut(s) 71, 509, 737, 751
AluI AGCT 4 cut(s) 71, 509, 737, 751
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 2 cut(s) 261, 274
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 5 cut(s) 502, 537, 574, 666, 720
ApeKI GCWGC 2 cut(s) 344, 509
ApoI RAATTY 1 cut(s) 688
AspS9I GGNCC 1 cut(s) 575
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 3 cut(s) 70, 80, 698
BaeGI GKGCMC 1 cut(s) 529
BaeI ACNNNNGTAYC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 504
BamHI GGATCC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 2 cut(s) 356, 496
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 2 cut(s) 45, 573
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 2 cut(s) 104, 147
BfmI CTRYAG 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 612
BglI GCCNNNNNGGC 1 cut(s) 533
BisI GCNGC 3 cut(s) 345, 510, 537
BlsI GCNGC 3 cut(s) 346, 511, 538
Bme1390I CCNGG 3 cut(s) 45, 573, 604
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 2 cut(s) 268, 526
BmrFI CCNGG 3 cut(s) 45, 573, 604
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 2 cut(s) 356, 558
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 67
BpuMI CCSGG 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 91, 669, 723
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 5 cut(s) 203, 224, 321, 475, 535
Bse1I ACTGG 4 cut(s) 163, 466, 537, 578
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 45, 573
BseDI CCNNGG 3 cut(s) 91, 669, 723
BseGI GGATG 2 cut(s) 37, 612
BseLI CCNNNNNNNGG 5 cut(s) 203, 224, 321, 475, 535
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 4 cut(s) 163, 466, 537, 578
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 2 cut(s) 356, 496
BshFI GGCC 5 cut(s) 504, 539, 576, 668, 722
BshNI GGYRCC 1 cut(s) 524
BsiSI CCGG 2 cut(s) 134, 603
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 5 cut(s) 203, 224, 321, 475, 535
BsmAI GTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 5 cut(s) 504, 539, 576, 668, 722
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 4 cut(s) 36, 49, 266, 657
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 536, 594
BspANI GGCC 5 cut(s) 504, 539, 576, 668, 722
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 268, 526
BspMAI CTGCAG 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 612
BspPI GGATC 2 cut(s) 261, 274
BspT107I GGYRCC 1 cut(s) 524
BspTNI GGTCTC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 4 cut(s) 163, 466, 537, 578
BssECI CCNNGG 3 cut(s) 91, 669, 723
BssMI GATC 4 cut(s) 36, 49, 266, 657
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 2 cut(s) 91, 723
Bst2UI CCWGG 2 cut(s) 45, 573
Bst4CI ACNGT 4 cut(s) 203, 377, 434, 614
Bst6I CTCTTC 1 cut(s) 711
BstACI GRCGYC 1 cut(s) 417
BstDEI CTNAG 2 cut(s) 11, 257
BstDSI CCRYGG 2 cut(s) 91, 669
BstEII GGTNACC 1 cut(s) 292
BstF5I GGATG 2 cut(s) 37, 612
BstKTI GATC 4 cut(s) 39, 52, 269, 660
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 36, 49, 266, 657
BstMWI GCNNNNNNNGC 2 cut(s) 249, 533
BstNI CCWGG 2 cut(s) 45, 573
BstNSI RCATGY 1 cut(s) 336
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 43, 571, 602
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 2 cut(s) 356, 496
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 266
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 5 cut(s) 504, 539, 576, 668, 722
BtgI CCRYGG 2 cut(s) 91, 669
BtsCI GGATG 2 cut(s) 37, 612
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 3 cut(s) 156, 339, 530
BveI ACCTGC 1 cut(s) 612
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 320, 610
CviAII CATG 5 cut(s) 92, 240, 333, 541, 710
CviQI GTAC 2 cut(s) 320, 610
DdeI CTNAG 2 cut(s) 11, 257
DpnI GATC 4 cut(s) 38, 51, 268, 659
DpnII GATC 4 cut(s) 36, 49, 266, 657
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 3 cut(s) 502, 537, 666
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Eco130I CCWWGG 2 cut(s) 91, 723
Eco31I GGTCTC 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 43, 571
EcoT14I CCWWGG 2 cut(s) 91, 723
ErhI CCWWGG 2 cut(s) 91, 723
FaeI CATG 5 cut(s) 95, 243, 336, 544, 713
FaiI YATR 9 cut(s) 93, 171, 241, 288, 334, 383, 542, 617, 711
FaqI GGGAC 1 cut(s) 171
FatI CATG 5 cut(s) 91, 239, 332, 540, 709
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 345, 510, 537
FokI GGATG 2 cut(s) 44, 619
Fsp4HI GCNGC 3 cut(s) 345, 510, 537
FspBI CTAG 2 cut(s) 104, 147
GluI GCNGC 3 cut(s) 345, 510, 537
HaeIII GGCC 5 cut(s) 504, 539, 576, 668, 722
HapII CCGG 2 cut(s) 134, 603
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 5 cut(s) 95, 243, 336, 544, 713
HinfI GANTC 5 cut(s) 173, 207, 353, 362, 489
HpaII CCGG 2 cut(s) 134, 603
HphI GGTGA 3 cut(s) 70, 80, 698
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 4 cut(s) 352, 396, 494, 647
Hpy188III TCNNGA 3 cut(s) 197, 256, 469
Hpy8I GTNNAC 1 cut(s) 699
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 4 cut(s) 203, 377, 434, 614
HpyCH4IV ACGT 2 cut(s) 417, 560
HpyCH4V TGCA 5 cut(s) 124, 336, 344, 446, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 533
HpyF3I CTNAG 2 cut(s) 11, 257
HpySE526I ACGT 2 cut(s) 417, 560
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 5 cut(s) 95, 243, 336, 544, 713
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 4 cut(s) 36, 49, 266, 657
LmnI GCTCC 1 cut(s) 742
Lsp1109I GCAGC 2 cut(s) 356, 496
LweI GCATC 2 cut(s) 356, 558
MaeI CTAG 2 cut(s) 104, 147
MaeII ACGT 2 cut(s) 417, 560
MaeIII GTNAC 2 cut(s) 14, 292
MalI GATC 4 cut(s) 38, 51, 268, 659
MboI GATC 4 cut(s) 36, 49, 266, 657
MboII GAAGA 6 cut(s) 67, 380, 383, 507, 540, 728
MflI RGATCY 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 529
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 3 cut(s) 303, 409, 688
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 2 cut(s) 347, 498
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 8 cut(s) 21, 101, 254, 430, 564, 667, 706, 712
Mox20I TGGCCA 1 cut(s) 504
MscI TGGCCA 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 2 cut(s) 134, 603
MspR9I CCNGG 3 cut(s) 45, 573, 604
MvaI CCWGG 2 cut(s) 45, 573
MwoI GCNNNNNNNGC 2 cut(s) 249, 533
NciI CCSGG 1 cut(s) 604
NcoI CCATGG 1 cut(s) 91
NdeII GATC 4 cut(s) 36, 49, 266, 657
NlaIII CATG 5 cut(s) 95, 243, 336, 544, 713
NlaIV GGNNCC 2 cut(s) 268, 526
NspI RCATGY 1 cut(s) 336
PfeI GAWTC 3 cut(s) 173, 207, 362
PkrI GCNGC 3 cut(s) 346, 511, 538
PleI GAGTC 2 cut(s) 347, 497
PpsI GAGTC 2 cut(s) 347, 497
Psp1406I AACGTT 1 cut(s) 560
Psp6I CCWGG 2 cut(s) 43, 571
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 2 cut(s) 43, 571
PspN4I GGNNCC 2 cut(s) 268, 526
PspPI GGNCC 1 cut(s) 575
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 2 cut(s) 321, 611
RsaNI GTAC 2 cut(s) 320, 610
SatI GCNGC 3 cut(s) 345, 510, 537
Sau3AI GATC 4 cut(s) 36, 49, 266, 657
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 2 cut(s) 347, 498
ScrFI CCNGG 3 cut(s) 45, 573, 604
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 2 cut(s) 356, 558
SfcI CTRYAG 1 cut(s) 342
Sse9I AATT 3 cut(s) 303, 409, 688
SsiI CCGC 2 cut(s) 536, 594
SspMI CTAG 2 cut(s) 104, 147
StyD4I CCNGG 3 cut(s) 43, 571, 602
StyI CCWWGG 2 cut(s) 91, 723
TaaI ACNGT 4 cut(s) 203, 377, 434, 614
TaiI ACGT 2 cut(s) 420, 563
TaqI TCGA 2 cut(s) 246, 629
TasI AATT 3 cut(s) 303, 409, 688
TatI WGTACW 1 cut(s) 609
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 3 cut(s) 173, 207, 362
TscAI CASTG 3 cut(s) 163, 346, 537
TseI GCWGC 2 cut(s) 344, 509
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 3 cut(s) 163, 346, 537
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 336
XspI CTAG 2 cut(s) 104, 147
ZraI GACGTC 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.