Rorug03G0076900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001240
Physical Location & Seq
Reverse (-)
5998747 .. 6000287
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0076900.1

Sequence Viewer

Length: 996 bp
ATGAAGTCTCTTATTCTTATTATTTCAGTGATATTGGCTCTTTTCTCTTTTCTGAATGGATTGGATGCAATCCGTCCACTAGACCAAGTTTCAACTACCATCGTAGTTGATCAGAATGGTAAAGGACAATTTACCACTGTTCAACAAGCCGTCGACTCTATACCACCAAATAACCCCGCGTGGACTCTAATTCACCTCAACTCCGGTGTCTACAATGAAAAAGTTGAGATCAAGACACCATATATTATCCTTGAAGGAGACCGCGATCAGCAAACTGTGATTCAATTTGGAGATGGCGGAAGTGTTGTTACCAGTCCTACATTTAAGTTGAATGCAGATAATTTTGTGGCACGATATATTCTATTCAAGAATACCTATGATCGTCTTCCTTCTAAATTAGCCGCCAACGGGGTGAACACTACTTGGGCACCTGCAGCTGCAATTGCCGGAGACAGAGCAAGTTTTTACCATTGCAGCTTCATTAGCTTGCAGGACACATTGACTGATGCCAAAGGTCGTCATTACTTCTATGACTGCCAAATTTCCGGCGCCATAGACTTCATTTGGGGAAATGGACAATCAATTTACGAGAATTGCAAGATCAATTCATTGGCATCAAAAATAGCTCCACGGGTAGGTTTTATCACAGCACAGGCTCGTGAAAGTCCAGAGGAAAGTACTGGTTTTGTATTCAAAAATTGTCATGTGACTGGATCTGGCCCTATACATCTAGGAAGAGCTCATACAAACTACGCAAGAGTATTGTTTGCTGGGACAACAATGGACAATGTTATTACGCCAGAAGGTTGGGACGGGGCACGGTTTAAGGGCTCCGAGAATTTAATCTCCTTCTCGGAGGCCAATTGCAGGGGACCTGGAGCAAACATGTCGAAACGTGTAAACTGGGAGAAGAAACTATCAGATCAAGAAGTAAATGATTTGACTAGTTTATCTTACATAAATCAAGATGGATGGATAGAGAAGCAACCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

36.47

Weight (kDa)

6.31

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 34 - 322 4e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 439
AccB1I GGYRCC 2 cut(s) 427, 548
AccI GTMKAC 2 cut(s) 153, 210
AccII CGCG 2 cut(s) 179, 264
AciI CCGC 4 cut(s) 177, 262, 297, 402
AclWI GGATC 1 cut(s) 721
AcsI RAATTY 2 cut(s) 540, 838
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 1 cut(s) 679
AfiI CCNNNNNNNGG 3 cut(s) 408, 635, 867
AflIII ACRYGT 2 cut(s) 885, 895
AgsI TTSAA 7 cut(s) 93, 143, 254, 284, 331, 367, 694
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 1 cut(s) 874
AluBI AGCT 5 cut(s) 437, 477, 486, 626, 740
AluI AGCT 5 cut(s) 437, 477, 486, 626, 740
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 3 cut(s) 12, 252, 444
AlwI GGATC 1 cut(s) 721
AoxI GGCC 2 cut(s) 718, 858
ApeKI GCWGC 3 cut(s) 434, 437, 474
ApoI RAATTY 2 cut(s) 540, 838
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 2 cut(s) 719, 872
AsuHPI GGTGA 2 cut(s) 185, 424
AvaII GGWCC 1 cut(s) 872
BaeGI GKGCMC 2 cut(s) 430, 820
BanI GGYRCC 2 cut(s) 427, 548
BanII GRGCYC 2 cut(s) 742, 833
BarI GAAGNNNNNNTAC 4 cut(s) 292, 324, 727, 759
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 3 cut(s) 424, 446, 486
BccI CCATC 4 cut(s) 107, 287, 962, 966
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 870, 904
BciT130I CCWGG 1 cut(s) 876
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 3 cut(s) 12, 252, 444
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 4 cut(s) 80, 731, 945, 990
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 552
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 4 cut(s) 402, 435, 438, 475
BlsI GCNGC 4 cut(s) 403, 436, 439, 476
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 1 cut(s) 876
Bme18I GGWCC 1 cut(s) 872
BmgT120I GGNCC 2 cut(s) 719, 872
BmiI GGNNCC 4 cut(s) 429, 550, 832, 873
BmrFI CCNGG 1 cut(s) 876
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 3 cut(s) 55, 496, 623
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 897
BsaHI GRCGYC 1 cut(s) 549
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 629
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 408, 635, 867
Bse1I ACTGG 4 cut(s) 312, 685, 715, 908
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 1 cut(s) 629
BseGI GGATG 2 cut(s) 70, 977
BseLI CCNNNNNNNGG 3 cut(s) 408, 635, 867
BseMI GCAATG 1 cut(s) 469
BseNI ACTGG 4 cut(s) 312, 685, 715, 908
BseSI GKGCMC 2 cut(s) 430, 820
BseXI GCAGC 3 cut(s) 424, 446, 486
BseYI CCCAGC 1 cut(s) 770
Bsh1236I CGCG 2 cut(s) 179, 264
BshFI GGCC 2 cut(s) 720, 860
BshNI GGYRCC 2 cut(s) 427, 548
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 3 cut(s) 204, 447, 546
BslFI GGGAC 3 cut(s) 787, 824, 885
BslI CCNNNNNNNGG 3 cut(s) 408, 635, 867
BsmAI GTCTC 3 cut(s) 12, 252, 444
BsmFI GGGAC 3 cut(s) 787, 824, 885
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 2 cut(s) 720, 860
Bso31I GGTCTC 1 cut(s) 252
Bsp1286I GDGCHC 4 cut(s) 430, 742, 820, 833
Bsp143I GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
BspACI CCGC 4 cut(s) 177, 262, 297, 402
BspANI GGCC 2 cut(s) 720, 860
BspFNI CGCG 2 cut(s) 179, 264
BspLI GGNNCC 4 cut(s) 429, 550, 832, 873
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 439
BspPI GGATC 1 cut(s) 721
BspQI GCTCTTC 1 cut(s) 730
BspT107I GGYRCC 2 cut(s) 427, 548
BspTNI GGTCTC 1 cut(s) 252
BsrDI GCAATG 1 cut(s) 469
BsrI ACTGG 4 cut(s) 312, 685, 715, 908
BssECI CCNNGG 1 cut(s) 629
BssMI GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
BssNI GRCGYC 1 cut(s) 549
BssSI CACGAG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 3 cut(s) 139, 277, 822
Bst6I CTCTTC 1 cut(s) 730
BstACI GRCGYC 1 cut(s) 549
BstC8I GCNNGC 1 cut(s) 488
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 70, 977
BstFNI CGCG 2 cut(s) 179, 264
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 7 cut(s) 112, 231, 268, 382, 603, 716, 925
BstMAI GTCTC 3 cut(s) 12, 252, 444
BstMBI GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
BstMWI GCNNNNNNNGC 3 cut(s) 434, 443, 483
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 1 cut(s) 889
BstSCI CCNGG 1 cut(s) 874
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 2 cut(s) 430, 820
BstUI CGCG 2 cut(s) 179, 264
BstV1I GCAGC 3 cut(s) 424, 446, 486
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 713
BstXI CCANNNNNNTGG 1 cut(s) 807
BstYI RGATCY 1 cut(s) 713
BsuRI GGCC 2 cut(s) 720, 860
BtgI CCRYGG 1 cut(s) 629
BtsCI GGATG 2 cut(s) 70, 977
BtsIMutI CAGTG 2 cut(s) 33, 135
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 1 cut(s) 488
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 2 cut(s) 719, 872
Csp6I GTAC 1 cut(s) 678
CviAII CATG 2 cut(s) 704, 886
CviQI GTAC 1 cut(s) 678
DinI GGCGCC 1 cut(s) 550
DpnI GATC 7 cut(s) 111, 230, 267, 381, 602, 715, 924
DpnII GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
Eam1104I CTCTTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 730
EciI GGCGGA 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 740
Eco24I GRGCYC 2 cut(s) 742, 833
Eco31I GGTCTC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 872
Eco53kI GAGCTC 1 cut(s) 740
EcoICRI GAGCTC 1 cut(s) 740
EcoO109I RGGNCCY 1 cut(s) 872
EcoRII CCWGG 1 cut(s) 874
EcoT38I GRGCYC 2 cut(s) 742, 833
EgeI GGCGCC 1 cut(s) 550
EheI GGCGCC 1 cut(s) 550
FaeI CATG 2 cut(s) 707, 889
FaqI GGGAC 3 cut(s) 787, 824, 885
FatI CATG 2 cut(s) 703, 885
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 2 cut(s) 153, 210
Fnu4HI GCNGC 4 cut(s) 402, 435, 438, 475
FokI GGATG 2 cut(s) 77, 984
FriOI GRGCYC 2 cut(s) 742, 833
Fsp4HI GCNGC 4 cut(s) 402, 435, 438, 475
FspBI CTAG 4 cut(s) 80, 731, 945, 990
GlaI GCGC 1 cut(s) 550
GluI GCNGC 4 cut(s) 402, 435, 438, 475
GsaI CCCAGC 1 cut(s) 774
GsuI CTGGAG 1 cut(s) 897
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 2 cut(s) 720, 860
HapII CCGG 3 cut(s) 204, 447, 546
HhaI GCGC 1 cut(s) 551
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 2 cut(s) 707, 889
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 3 cut(s) 155, 184, 280
HpaII CCGG 3 cut(s) 204, 447, 546
HphI GGTGA 2 cut(s) 185, 424
Hpy166II GTNNAC 6 cut(s) 77, 154, 183, 211, 415, 901
Hpy188I TCNGA 5 cut(s) 54, 114, 835, 856, 922
Hpy188III TCNNGA 6 cut(s) 232, 367, 659, 668, 926, 965
Hpy8I GTNNAC 6 cut(s) 77, 154, 183, 211, 415, 901
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 248, 399, 797, 859
HpyCH4III ACNGT 3 cut(s) 139, 277, 822
HpyCH4IV ACGT 1 cut(s) 895
HpyCH4V TGCA 8 cut(s) 68, 335, 434, 440, 474, 490, 597, 867
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 443, 483
HpySE526I ACGT 1 cut(s) 895
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 2 cut(s) 707, 889
HspAI GCGC 1 cut(s) 549
KasI GGCGCC 1 cut(s) 548
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 3 cut(s) 631, 836, 878
Lsp1109I GCAGC 3 cut(s) 424, 446, 486
LweI GCATC 3 cut(s) 55, 496, 623
MaeI CTAG 4 cut(s) 80, 731, 945, 990
MaeII ACGT 1 cut(s) 895
MaeIII GTNAC 2 cut(s) 307, 706
MalI GATC 7 cut(s) 111, 230, 267, 381, 602, 715, 924
MboI GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
MboII GAAGA 3 cut(s) 377, 747, 922
MfeI CAATTG 2 cut(s) 441, 862
MflI RGATCY 1 cut(s) 713
MhlI GDGCHC 4 cut(s) 430, 742, 820, 833
Mly113I GGCGCC 1 cut(s) 549
MlyI GAGTC 2 cut(s) 149, 178
MnlI CCTC 3 cut(s) 206, 664, 850
MseI TTAA 3 cut(s) 324, 825, 842
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 3 cut(s) 204, 447, 546
MspR9I CCNGG 1 cut(s) 876
MunI CAATTG 2 cut(s) 441, 862
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 2 cut(s) 179, 264
MwoI GCNNNNNNNGC 3 cut(s) 434, 443, 483
NarI GGCGCC 1 cut(s) 549
NdeII GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
NlaIII CATG 2 cut(s) 707, 889
NlaIV GGNNCC 4 cut(s) 429, 550, 832, 873
NmuCI GTSAC 1 cut(s) 706
NspI RCATGY 1 cut(s) 889
PaqCI CACCTGC 1 cut(s) 439
PciI ACATGT 1 cut(s) 885
PciSI GCTCTTC 1 cut(s) 730
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 4 cut(s) 403, 436, 439, 476
PleI GAGTC 2 cut(s) 149, 178
PluTI GGCGCC 1 cut(s) 552
PpsI GAGTC 2 cut(s) 149, 178
PpuMI RGGWCCY 1 cut(s) 872
PscI ACATGT 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 742
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 770
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 4 cut(s) 429, 550, 832, 873
PspPI GGNCC 2 cut(s) 719, 872
PspPPI RGGWCCY 1 cut(s) 872
PstI CTGCAG 1 cut(s) 436
PsuI RGATCY 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 1 cut(s) 679
RsaNI GTAC 1 cut(s) 678
SacI GAGCTC 1 cut(s) 742
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 3 cut(s) 324, 825, 842
SatI GCNGC 4 cut(s) 402, 435, 438, 475
Sau3AI GATC 7 cut(s) 109, 228, 265, 379, 600, 713, 922
Sau96I GGNCC 2 cut(s) 719, 872
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 2 cut(s) 149, 178
ScrFI CCNGG 1 cut(s) 876
SduI GDGCHC 4 cut(s) 430, 742, 820, 833
SfaNI GCATC 3 cut(s) 55, 496, 623
SfcI CTRYAG 1 cut(s) 432
SfoI GGCGCC 1 cut(s) 550
SinI GGWCC 1 cut(s) 872
SpeI ACTAGT 1 cut(s) 944
SsiI CCGC 4 cut(s) 177, 262, 297, 402
SspDI GGCGCC 1 cut(s) 548
SspMI CTAG 4 cut(s) 80, 731, 945, 990
SstI GAGCTC 1 cut(s) 742
StyD4I CCNGG 1 cut(s) 874
TaaI ACNGT 3 cut(s) 139, 277, 822
TaiI ACGT 1 cut(s) 898
TaqI TCGA 2 cut(s) 153, 890
TatI WGTACW 1 cut(s) 677
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 3 cut(s) 324, 825, 842
Tru9I TTAA 3 cut(s) 324, 825, 842
TscAI CASTG 2 cut(s) 33, 142
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 3 cut(s) 434, 437, 474
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 5 cut(s) 17, 231, 469, 550, 597
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 2 cut(s) 33, 142
VpaK11BI GGWCC 1 cut(s) 872
XapI RAATTY 2 cut(s) 540, 838
XceI RCATGY 1 cut(s) 889
XmiI GTMKAC 2 cut(s) 153, 210
XspI CTAG 4 cut(s) 80, 731, 945, 990
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.