RchiOBHm_Chr4g0408821

Extensin-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001240
Physical Location & Seq
Reverse (-)
31684083 .. 31687531
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37990

Sequence Viewer

Length: 2412 bp
ATGGCTTACGGTAAAACCAAGAACAACGGTAAATTCAGCGTCGCAGTCGAAGATTTTGACTACGGTAAATATGGTGCCAAGGCTTGTACCGCGAAGCTCCACATGGCACCCAAGGGTTCACTCTGCAGCATTCCAACTCCTCTCAACTGGGGCGACAAGGGAGCCAAGCTGAAGGTCAAGTCCAAGAACAATTGGGAGGTTGTGCTCAAGGCCAAGCCCTTTGCTTTCGCTCCAAAGACCCCTTACAAGGAGTGTGCTAAGCCCGCTCCACACCATCCTTCTCCTTACGTCTACAAGTCGCCGCCTCCACCAACCCCTACTTACGTCTACAAATCTCCTCCTCCACCGTCGCCAAAGTACATTTACAAGTCGCCGCCACCGCCGGCTTACTACTACAAGTCGCCACCACCACCATCACCAAAGCCGTATGTGTACAAGTCTCCACCACCACCATCTCCCAAGCCGTACGTGTACAAGTCTCCTCCCCCACCATCTCCTAAGCCGTACGTGTACAAGTCTCCACCGCCACCATCTCCCAAGCCGTACGTCTACAAGTCTCCACCGCCACCATCTCCCAAGCCGTACGTGTACAAGTCTCCTCCACCACCATCACCGAAGCCGTACGTGTACAAGTCTCCACCACCACCATCTCCGAAGCCGTACGTGTACAAGTCTCCTCCACCACCATCACCGAAGCCGTACGTGTACAAGTCTCCACCACCACCATCTCCGAAGCCGTACGTGTACAAGTCTCCACCACCACCATCTCCCAAGCCGTACGAGTACAAGTCTCCTCCACCACCATCACCCAAGCCGTACGTGTACAAGTCTCCACCGCCACCGCCACCAAAGTACTACTACAAGTCTCCACCACCACCCACATACATCTACAAGTCACCACCACCACCATCACCGAAGCCATACATCTACAAGTCTCCACCACCACCACCACCAAAGTACTACTACAAGTCTCCACCACCACCACCACCAAAGTACTACTACAAGTCTCCACCCCCACCAACTTACGTATACAAGTCACCACCACCACCCACATACATCTACAAGTCACCACCACCACCATCACCTACACCTTACGTGTACAAGTCTCCACCACCACCACCACCAAAGTACTACTACAAGTCACCACCGCCACCAACTTATATCTACAAGTCACCTCCACCACCAGCATATATCTACAAGTCACCACCACCACCACATTCTCACCCTCACCCACCCTACATTTACAAGTCTCCACCACCACCTTCACCATCTCCTCCTCCTCCATACTACTACAAATCCCCACCACCACCACCACCATACATCTACAAGTCTCCTCCACCTCCATCACCTTCTCCTCCACCTCCATACTACTACAAATCACCACCACCACCATCACCCTCTCCCCCACATCCATACTACTACAAATCTCCCCTCCACCATCACCTTCTCCTCCTCCACCTTATATCTACAAGTCACCCCCACCACCGTCACCCTCTCCTCCCCCTCCATACTACTACAAATCTCCCCCTCCACCATACGTCTACAAGTCCCCTCCTCCTCCCTCACCCTCACCTCCACCACCATATTTATACAAATCTCCACCACCACCCTCGTCGTCTCCCCCTCCACCATACCACTACACCTCCCCGCCGCCACCTTCCCCATCTCCTCCACCACCATACCACTACACCTCACCACCACCGCCCTCTCCATCTCCTCCCCCACCATACCAATACAAGTCTCCACCTCCCCCACACAAGGAACCAAAGCCAACTCCTCCCTACCATTACACATCTCCACCACCACCATCGCCATCTCCTCCACCACCATACCACTACACCTCACCACCACCACCCTCTCCATCTCCTCCCCACCATACCAATACAAGTCTCCACCTCCCCCACACAAGGAACCAAAGCCAACTCCTCCCTACCATTACACATCTCCACCACCACCATCGCCATCTCCTCCTCCACCATACCACTACACCTCCCCACCTCCACCTTCCCCATCTCCTCCCCCACCATACGCTTACAAGTCTCCACCACCTCCTTCACCTTCTCCTCCTCCTCCCTACGTCTACAAGTCACCTCCACCTCCGGTGAAATCTCCCCCACCACCGGTGTACATTTACGCCTCTCCCCCACCACCCACTCACTACTAAGCTCAAACACATGCATAGACCAATCATGTTTTAATTTAAGCAGCAGAATAAAGGAAGGCTTCATGAGCCGAATACTGAGACACCAAGCTTCCAATCCAAGTCCATTGTTATTGATATTGGTGATCAAAAAGTGTCTGGCAGAAACATCAGCTATGACCAGCAACCTCATCTTGCACATGCTCTTATTATTCTCATCATCCAAAATGGGAATTCAAAGCAAGATTGATCTTGGTCTTGGAAGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Extensin_2 Pollen_Ole_e_I

Protein Analysis

803

Amino Acids

89.97

Weight (kDa)

9.93

Isoelectric Point (pI)

87.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 74, 106
AccBSI CCGCTC 1 cut(s) 266
AccI GTMKAC 6 cut(s) 291, 327, 549, 1029, 1541, 2080
AccII CGCG 1 cut(s) 92
AcsI RAATTY 2 cut(s) 32, 2373
AcuI CTGAAG 1 cut(s) 191
AfiI CCNNNNNNNGG 4 cut(s) 247, 1758, 1907, 2120
AflIII ACRYGT 9 cut(s) 468, 507, 585, 624, 663, 702, 741, 819, 1095
AgeI ACCGGT 1 cut(s) 2120
AgsI TTSAA 1 cut(s) 2378
AluBI AGCT 5 cut(s) 97, 169, 2166, 2252, 2315
AluI AGCT 5 cut(s) 97, 169, 2166, 2252, 2315
Alw21I GWGCWC 1 cut(s) 207
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 126, 2205
ApoI RAATTY 2 cut(s) 32, 2373
AsiGI ACCGGT 1 cut(s) 2120
BanI GGYRCC 2 cut(s) 74, 106
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 138, 2217
BclI TGATCA 1 cut(s) 2286
BfmI CTRYAG 1 cut(s) 124
BisI GCNGC 5 cut(s) 127, 302, 374, 1651, 2206
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 5 cut(s) 128, 303, 375, 1652, 2207
BmcAI AGTACT 4 cut(s) 854, 959, 995, 1130
BmiI GGNNCC 5 cut(s) 76, 108, 163, 1763, 1912
BmrI ACTGGG 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 157
Bpu10I CCTNAGC 1 cut(s) 498
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 191
BsaJI CCNNGG 2 cut(s) 78, 111
BsaWI WCCGGW 2 cut(s) 2099, 2120
BsaXI ACNNNNNCTCC 4 cut(s) 1627, 1657, 1974, 2004
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 1758, 1907, 2120
Bse118I RCCGGY 2 cut(s) 382, 2120
Bse1I ACTGG 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 78, 111
BseGI GGATG 3 cut(s) 274, 1408, 2360
BseLI CCNNNNNNNGG 4 cut(s) 247, 1758, 1907, 2120
BseMII CTCAG 1 cut(s) 2231
BseNI ACTGG 1 cut(s) 152
BseXI GCAGC 2 cut(s) 138, 2217
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 2 cut(s) 74, 106
BshTI ACCGGT 1 cut(s) 2120
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 3 cut(s) 383, 2100, 2121
BslFI GGGAC 1 cut(s) 1533
BslI CCNNNNNNNGG 4 cut(s) 247, 1758, 1907, 2120
BsmBI CGTCTC 1 cut(s) 1622
BsmFI GGGAC 1 cut(s) 1533
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 2286, 2389
Bsp1720I GCTNAGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 1 cut(s) 2232
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 2226
BspLI GGNNCC 5 cut(s) 76, 108, 163, 1763, 1912
BspMAI CTGCAG 1 cut(s) 128
BspT107I GGYRCC 2 cut(s) 74, 106
BsrBI CCGCTC 1 cut(s) 266
BsrFI RCCGGY 2 cut(s) 382, 2120
BsrI ACTGG 1 cut(s) 152
BssAI RCCGGY 2 cut(s) 382, 2120
BssECI CCNNGG 2 cut(s) 78, 111
BssMI GATC 2 cut(s) 2286, 2389
BssNAI GTATAC 1 cut(s) 1030
BssT1I CCWWGG 2 cut(s) 78, 111
Bst1107I GTATAC 1 cut(s) 1030
Bst4CI ACNGT 5 cut(s) 11, 29, 65, 348, 1487
BstC8I GCNNGC 2 cut(s) 264, 384
BstDEI CTNAG 4 cut(s) 258, 498, 2162, 2240
BstF5I GGATG 3 cut(s) 274, 1408, 2360
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 2 cut(s) 2289, 2392
BstMBI GATC 2 cut(s) 2286, 2389
BstMWI GCNNNNNNNGC 4 cut(s) 89, 263, 379, 2229
BstNSI RCATGY 2 cut(s) 2178, 2344
BstSFI CTRYAG 1 cut(s) 124
BstSNI TACGTA 1 cut(s) 1027
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 138, 2217
BstZ17I GTATAC 1 cut(s) 1030
BsuRI GGCC 1 cut(s) 212
BtgZI GCGATG 2 cut(s) 1793, 1942
BtsCI GGATG 3 cut(s) 274, 1408, 2360
Cac8I GCNNGC 2 cut(s) 264, 384
CciI TCATGA 1 cut(s) 2226
Cfr10I RCCGGY 2 cut(s) 382, 2120
CseI GACGC 1 cut(s) 28
CspAI ACCGGT 1 cut(s) 2120
CviAII CATG 5 cut(s) 103, 2175, 2190, 2227, 2341
DdeI CTNAG 4 cut(s) 258, 498, 2162, 2240
DpnI GATC 2 cut(s) 2288, 2391
DpnII GATC 2 cut(s) 2286, 2389
Eco105I TACGTA 1 cut(s) 1027
Eco130I CCWWGG 2 cut(s) 78, 111
Eco57I CTGAAG 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 2373
EcoT14I CCWWGG 2 cut(s) 78, 111
EcoT22I ATGCAT 1 cut(s) 2180
ErhI CCWWGG 2 cut(s) 78, 111
Esp3I CGTCTC 1 cut(s) 1622
FaeI CATG 5 cut(s) 106, 2178, 2193, 2230, 2344
FalI AAGNNNNNCTT 2 cut(s) 2207, 2239
FaqI GGGAC 1 cut(s) 1533
FatI CATG 5 cut(s) 102, 2174, 2189, 2226, 2340
FauI CCCGC 2 cut(s) 271, 1655
FbaI TGATCA 1 cut(s) 2286
FblI GTMKAC 6 cut(s) 291, 327, 549, 1029, 1541, 2080
Fnu4HI GCNGC 5 cut(s) 127, 302, 374, 1651, 2206
FokI GGATG 3 cut(s) 261, 1395, 2347
Fsp4HI GCNGC 5 cut(s) 127, 302, 374, 1651, 2206
GluI GCNGC 5 cut(s) 127, 302, 374, 1651, 2206
HaeIII GGCC 1 cut(s) 212
HapII CCGG 3 cut(s) 383, 2100, 2121
HgaI GACGC 1 cut(s) 28
Hin1II CATG 5 cut(s) 106, 2178, 2193, 2230, 2344
HindIII AAGCTT 1 cut(s) 2250
HpaII CCGG 3 cut(s) 383, 2100, 2121
Hpy188I TCNGA 2 cut(s) 654, 732
Hpy188III TCNNGA 1 cut(s) 2227
Hpy99I CGWCG 3 cut(s) 44, 352, 1617
HpyCH4III ACNGT 5 cut(s) 11, 29, 65, 348, 1487
HpyCH4V TGCA 3 cut(s) 126, 2178, 2338
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 263, 379, 2229
HpyF3I CTNAG 4 cut(s) 258, 498, 2162, 2240
Hsp92II CATG 5 cut(s) 106, 2178, 2193, 2230, 2344
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Ksp22I TGATCA 1 cut(s) 2286
Kzo9I GATC 2 cut(s) 2286, 2389
LmnI GCTCC 4 cut(s) 102, 161, 235, 271
LpnPI CCDG 7 cut(s) 133, 396, 1197, 2113, 2134, 2285, 2335
Lsp1109I GCAGC 2 cut(s) 138, 2217
MaeIII GTNAC 9 cut(s) 894, 1035, 1065, 1140, 1170, 1200, 1472, 1487, 2086
MalI GATC 2 cut(s) 2288, 2391
MbiI CCGCTC 1 cut(s) 266
MboI GATC 2 cut(s) 2286, 2389
MboII GAAGA 1 cut(s) 62
MfeI CAATTG 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 4 cut(s) 32, 190, 2197, 2373
MmeI TCCRAC 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 2180
MroNI GCCGGC 1 cut(s) 382
MseI TTAA 2 cut(s) 2196, 2201
MspI CCGG 3 cut(s) 383, 2100, 2121
MunI CAATTG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 129
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 89, 263, 379, 2229
NaeI GCCGGC 1 cut(s) 384
NdeII GATC 2 cut(s) 2286, 2389
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 5 cut(s) 106, 2178, 2193, 2230, 2344
NlaIV GGNNCC 5 cut(s) 76, 108, 163, 1763, 1912
NmuCI GTSAC 9 cut(s) 894, 1035, 1065, 1140, 1170, 1200, 1472, 1487, 2086
NsiI ATGCAT 1 cut(s) 2180
NspI RCATGY 2 cut(s) 2178, 2344
PagI TCATGA 1 cut(s) 2226
PctI GAATGC 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 384
PinAI ACCGGT 1 cut(s) 2120
PkrI GCNGC 5 cut(s) 128, 303, 375, 1652, 2207
PspN4I GGNNCC 5 cut(s) 76, 108, 163, 1763, 1912
PstI CTGCAG 1 cut(s) 128
SaqAI TTAA 2 cut(s) 2196, 2201
SatI GCNGC 5 cut(s) 127, 302, 374, 1651, 2206
Sau3AI GATC 2 cut(s) 2286, 2389
ScaI AGTACT 4 cut(s) 854, 959, 995, 1130
SduI GDGCHC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 124
SgrAI CRCCGGYG 1 cut(s) 2120
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SnaBI TACGTA 1 cut(s) 1027
Sse9I AATT 4 cut(s) 32, 190, 2197, 2373
StyI CCWWGG 2 cut(s) 78, 111
TaaI ACNGT 5 cut(s) 11, 29, 65, 348, 1487
TaqI TCGA 1 cut(s) 48
TasI AATT 4 cut(s) 32, 190, 2197, 2373
TauI GCSGC 3 cut(s) 304, 376, 1653
Tru1I TTAA 2 cut(s) 2196, 2201
Tru9I TTAA 2 cut(s) 2196, 2201
TseFI GTSAC 9 cut(s) 894, 1035, 1065, 1140, 1170, 1200, 1472, 1487, 2086
TseI GCWGC 2 cut(s) 126, 2205
Tsp45I GTSAC 9 cut(s) 894, 1035, 1065, 1140, 1170, 1200, 1472, 1487, 2086
TspDTI ATGAA 1 cut(s) 2215
XapI RAATTY 2 cut(s) 32, 2373
XceI RCATGY 2 cut(s) 2178, 2344
XmiI GTMKAC 6 cut(s) 291, 327, 549, 1029, 1541, 2080
ZrmI AGTACT 4 cut(s) 854, 959, 995, 1130
Zsp2I ATGCAT 1 cut(s) 2180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.