Rroxscaffold_5G00353200

Extensin-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001240
Physical Location & Seq
Reverse (-)
30808139 .. 30818280
10142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353200.1

Sequence Viewer

Length: 3456 bp
ATGGCCGTGGCTTTTGCAGCCATAGTTGTCGTCTTTTCGAGCAATGTGGGTTCAGTTCAAGCTGATCATCCTTACCTTTACTCTTCGCCCCCACCGCCTTATGTCTACAGCTCGCCACCACCCCCTGCTCACTCTCCTCCACCACCGTACGTGTACAAGTCACCGCCTCCACCATCGCCTTCACCACCTCCTCCTTACGTGTACAAGTCACCTCCCCCACCATCGCCTTCACCACCTCCTCCTTACGTGTACAAGTCACCTCCCCCACCATCGCCTTCACCACCACCTCCTTACGTCTACAAGTCACCCCCTCCACCATCGCCTTCTCCACCACCGCCGTATGTGTACAAGTCTCCTCCACCTCCCTCACCTAAATTACCTCCGTACGAGTACAAGTCACCACCACCTCCATCACCTTCCCCCCACCACCATACGTCTACAACACATTCTCCACCACCACCTTACTATTACAAATCTCCTCCACCTCCAGCACATTCTCCACCACCACCTTACTACTATAAGTCTCCTCCTCCACCTTCTCCATCACCACCACCACCATACTACTACAAATCCCCGCCACCTCCTTCTCCCTCACCTCCTCCACCATACATCTACAAATCCCCTCCACCACCTTCCCCATCTCCTCCTCCACCATACTACTACAAGTCTCCTCCACCACCATCACCAAAGCCGTACGTGTACAAGTCTCCGCCGCCACCATCGCCGAAGCCTTACTACTACCAATCTCCTCCACCACCGGCTCCGTACTATCCACACCCACACCCCCACCACCAGCCATTGATCGTCAAGGTCGTCGGAAAAGTCTACTGCTACAGATGCTACGACTGGGGATATCCAATCAAGTCTCACGACAAGAAACATCTCGAAGGCGCTGTTGTTGAGATCACATGCTGGGCAGGCAAAAAGGCGATCGTGGCTTACGGTAAAACCAAGAACAACGGTAAATTCAGCGTCGCAGTCGAAGATTTTGACTACGGTAAATATGGTGCCAAGGCTTGTACCGCGAAGCTCCACATGGCACCCAAGGGTTCACTTTGCAGCATTCCAACTCCTCTCAACTGGGGCGACAAGGGAGCCAAGCTGAAGGTCAAGTCCAAGAACAATTGGGAGGTTGTGCTCAAGGCCAAGCCCTTTGCTTTCGCTCCAAAGACCCCTTACAAGGAGTTCGCCACCTCCACCAACCCCGACTTACGTCTACAAATCTCCTCCTCCACCGTCGCCAAAGTACATTTACAAGTCGCCGCCACCGCCGCTTACTACTACAAGTCGCCACCGCCGCCATCACCAAAGCCGTATGTGTACAAGTCTCCACCACCACCATCTCCCAAGCCGTACGTGTACAAGTCTCCTCCTCCACCATCTCCTAAGCCGTACGTATACAAGTCTCCACCGCCACCATCTCCGAAGCCGTACGTGTACAAGTCTCCTCCTCCACCATCTCCGAAGCCGTACGTGTACAAGTCTCCACCGCCACCATCTCCTAAGCCGTACGTGTACAAGTCTCCACCACCACCATCTCCCAAGCCGTACGTGTACAAGTCTCCACCGCCACCATCTCCCAAGCCGTACGTGTACAAGTCTCCACCGCCACCATCTCCGAAGCCGTACGTGTACAAGTCTCCTCCGCCACCATCTCCTAAGCCATACGTCTACAAGTCTCCACCACCACCATCTCCCAAGCCGTACGTGTACAAGTCTCCACCACCACCATCTCCTGCCGTACGTGTACAAGTCTCCACCACCACCATCTCCCAAGCCGTATGTGTACAAGTCTCCACCGCCACCGCCACCAAAGTACTACTACAAGTCTCCACCACCACCGCTTACTACTACAAGTCACCACCACCACCCACATACATCTACAAGTCACCACCACCACCATCACCGAAGCCATACATCTACAAGTCTCCACCACCACCACCACCAAAGTACTACTACAAGTCTCCACCCCCACCGACTTACGTATACAAGTCACCACCACCACCCACATACATCTACAAGTCACCACCACCACCATCACCTACACCTTACGTGTACAAGTCTCCACCACCACCACCACCAAAGTACTACTACAAGTCACCACCGCCACCGACTTATATCTACAAGTCACCACCACCACCCACATACATCTACAAGTCACCACCACCACCATCACCTACACCTTACGTGTACAAGTCTCCACCACCACCACCACCAAAGTACTACTACAAGTCACCACCGCCACCGACTTATATCTACAAGTCACCTCCACCTCCAGCATATATCTACAAGTCACCACCACCACCACATTCTCACCCTCACCCACCCTACATTTACAAGTCTCCACCACCACCTTCACCATCTCCTCCTCCTCCATACTACTACAAATCTCCACCACCACCCTCACCTTCTCCCCTCCTCCATACTACTACAATCTCCTCCACCTCCATCACCTTCTCCTCCACCTCCATACTACTACAAATCACCACCACCACCATCACCCTCTCCCCCACATCCATACTACTACAAATCTCCCCCTCCACCATCACCTTCTCCTCCTCCACCTTATATCTACAAGTCTCCCCCACCACCGTCACCCTCTCCTCCCCCTCCATACTACTACAAATCTCCCCTCCACCCTCACCATCTCCTCCTCCACCATACGTCTACAAGTCCCCTCCTCCTCCCTCACCCTCACCTCCACCACCATATTTATACAAATCTCCACCACCACCCTCATCGTCTCCCCCTCCACCATACCACTACACCTCACCACCACCACCCTCTCCATCTCCTCCCCCACCATACCAATACAAGTCTCCACCTCCCCCACACAAGGAACCAAAGCCAACTCCTCCCTACCATTACACATCTCCACCACCACCATCGCCATCTCCTCCTCCACCATACCACTACACCTCCCCACCTCCACCTTCCCCATCTCCTCCCCCACCATACGCTTACAAGTCTCCACCACCTCCTTCACCTTCTCCTCCTCCTCCCTACGTCTACAAGTCACCTCCACCTCCGGTGAAATCTCCCCCACCACCGGTCTGGTCAACAGCTCTCTTTTCGTGCGGGCGTGCCGGCACCATTAGGGTGCGGTCGTCGGGGGTGTCCCTTGACCTGATTTCTTTCAAGCGATTGTGGACGAGGAGAGCACCAACCTCGTCGTGGGATTCTTTGTGCCTGGTGGCGAGGACTTTTGCTGAAGTTTCTTCTTGCTCACAACGAAATCGAGGAAGGGAGGAAAGCCACTTTGAGGCCAAAGATCCTTCCTCGAACCCTTCCTTCGGAGTCCGACCATACCGAGTCCTCCGTATGAGAAGGGTTTTGGTGGTCGCCCTCTACTCATGCTCTCTCTTCCGAACATTGACGAATCTTAGAGGGAGAGCATGGATTCGCGGAAGTGATGGGTATGAGCCATTTTATGGCCGTTCCCATACTTCACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Extensin_2 Pollen_Ole_e_I

Protein Analysis

1151

Amino Acids

126.31

Weight (kDa)

9.79

Isoelectric Point (pI)

110.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Extensin_2 PF04554 22 - 58 4.8e-07 Extensin-like region
Pollen_Ole_e_1 PF01190 271 - 365 1.6e-15 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 1007, 1039, 3089
AccB7I CCANNNNNTGG 1 cut(s) 3431
AccII CGCG 2 cut(s) 1025, 3405
AclWI GGATC 1 cut(s) 3266
AcoI YGGCCR 2 cut(s) 3, 3433
AcsI RAATTY 1 cut(s) 965
AcuI CTGAAG 2 cut(s) 1124, 3231
AfiI CCNNNNNNNGG 7 cut(s) 1180, 2836, 3049, 3174, 3262, 3293, 3431
AgeI ACCGGT 1 cut(s) 3049
AgsI TTSAA 2 cut(s) 59, 3139
AjnI CCWGG 1 cut(s) 3189
AluBI AGCT 5 cut(s) 62, 111, 1030, 1102, 3065
AluI AGCT 5 cut(s) 62, 111, 1030, 1102, 3065
Alw21I GWGCWC 2 cut(s) 1140, 3163
AlwI GGATC 1 cut(s) 3266
AoxI GGCC 4 cut(s) 3, 1143, 3264, 3433
ApeKI GCWGC 2 cut(s) 17, 1059
ApoI RAATTY 1 cut(s) 965
AsiGI ACCGGT 1 cut(s) 3049
AspLEI GCGC 1 cut(s) 893
BanI GGYRCC 3 cut(s) 1007, 1039, 3089
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
Bbv12I GWGCWC 2 cut(s) 1140, 3163
BbvI GCAGC 2 cut(s) 29, 1071
BcgI CGANNNNNNTGC 2 cut(s) 3150, 3184
BciT130I CCWGG 1 cut(s) 3191
BclI TGATCA 1 cut(s) 64
BfmI CTRYAG 2 cut(s) 106, 832
BfoI RGCGCY 1 cut(s) 894
BisI GCNGC 6 cut(s) 18, 713, 1060, 1263, 1272, 1298
BlsI GCNGC 6 cut(s) 19, 714, 1061, 1264, 1273, 1299
BmcAI AGTACT 4 cut(s) 1818, 1952, 2087, 2222
Bme1390I CCNGG 1 cut(s) 3191
BmiI GGNNCC 6 cut(s) 762, 1009, 1041, 1096, 2841, 3091
BmrFI CCNGG 1 cut(s) 3191
BmrI ACTGGG 2 cut(s) 856, 1090
BmsI GCATC 1 cut(s) 827
BmuI ACTGGG 2 cut(s) 856, 1090
BoxI GACNNNNGTC 1 cut(s) 1212
BpmI CTGGAG 2 cut(s) 471, 2259
Bpu10I CCTNAGC 3 cut(s) 1386, 1503, 1659
BpuEI CTTGAG 1 cut(s) 1124
BsaJI CCNNGG 3 cut(s) 6, 1011, 1044
BsaWI WCCGGW 2 cut(s) 3028, 3049
BsaXI ACNNNNNCTCC 4 cut(s) 433, 463, 2903, 2933
Bsc4I CCNNNNNNNGG 7 cut(s) 1180, 2836, 3049, 3174, 3262, 3293, 3431
Bse118I RCCGGY 3 cut(s) 757, 3049, 3086
Bse1I ACTGG 2 cut(s) 851, 1085
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 1 cut(s) 3191
BseDI CCNNGG 3 cut(s) 6, 1011, 1044
BseGI GGATG 2 cut(s) 67, 2513
BseLI CCNNNNNNNGG 7 cut(s) 1180, 2836, 3049, 3174, 3262, 3293, 3431
BseMI GCAATG 1 cut(s) 49
BseNI ACTGG 2 cut(s) 851, 1085
BseXI GCAGC 2 cut(s) 29, 1071
BseYI CCCAGC 1 cut(s) 912
Bsh1236I CGCG 2 cut(s) 1025, 3405
Bsh1285I CGRYCG 2 cut(s) 933, 3107
BshFI GGCC 4 cut(s) 5, 1145, 3266, 3435
BshNI GGYRCC 3 cut(s) 1007, 1039, 3089
BshTI ACCGGT 1 cut(s) 3049
BsiEI CGRYCG 2 cut(s) 933, 3107
BsiHKAI GWGCWC 2 cut(s) 1140, 3163
BsiSI CCGG 4 cut(s) 758, 3029, 3050, 3087
BslFI GGGAC 2 cut(s) 2659, 3103
BslI CCNNNNNNNGG 7 cut(s) 1180, 2836, 3049, 3174, 3262, 3293, 3431
BsmBI CGTCTC 1 cut(s) 2748
BsmFI GGGAC 2 cut(s) 2659, 3103
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 4 cut(s) 5, 1145, 3266, 3435
Bsp1286I GDGCHC 2 cut(s) 1140, 3163
Bsp143I GATC 5 cut(s) 64, 801, 903, 930, 3271
BspANI GGCC 4 cut(s) 5, 1145, 3266, 3435
BspFNI CGCG 2 cut(s) 1025, 3405
BspLI GGNNCC 6 cut(s) 762, 1009, 1041, 1096, 2841, 3091
BspPI GGATC 1 cut(s) 3266
BspT107I GGYRCC 3 cut(s) 1007, 1039, 3089
BsrDI GCAATG 1 cut(s) 49
BsrFI RCCGGY 3 cut(s) 757, 3049, 3086
BsrI ACTGG 2 cut(s) 851, 1085
BssAI RCCGGY 3 cut(s) 757, 3049, 3086
BssECI CCNNGG 3 cut(s) 6, 1011, 1044
BssMI GATC 5 cut(s) 64, 801, 903, 930, 3271
BssNAI GTATAC 2 cut(s) 1399, 1987
BssT1I CCWWGG 2 cut(s) 1011, 1044
Bst1107I GTATAC 2 cut(s) 1399, 1987
Bst2UI CCWGG 1 cut(s) 3191
Bst4CI ACNGT 6 cut(s) 147, 944, 962, 998, 1237, 2593
Bst6I CTCTTC 2 cut(s) 88, 3368
BstC8I GCNNGC 5 cut(s) 113, 919, 3080, 3084, 3088
BstDEI CTNAG 4 cut(s) 1386, 1503, 1659, 3383
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 67, 2513
BstFNI CGCG 2 cut(s) 1025, 3405
BstH2I RGCGCY 1 cut(s) 894
BstHHI GCGC 1 cut(s) 893
BstKTI GATC 5 cut(s) 67, 804, 906, 933, 3274
BstMBI GATC 5 cut(s) 64, 801, 903, 930, 3271
BstMCI CGRYCG 2 cut(s) 933, 3107
BstNI CCWGG 1 cut(s) 3191
BstNSI RCATGY 1 cut(s) 912
BstPAI GACNNNNGTC 1 cut(s) 1212
BstSCI CCNGG 1 cut(s) 3189
BstSFI CTRYAG 2 cut(s) 106, 832
BstSNI TACGTA 2 cut(s) 1396, 1984
BstUI CGCG 2 cut(s) 1025, 3405
BstV1I GCAGC 2 cut(s) 29, 1071
BstX2I RGATCY 1 cut(s) 3271
BstXI CCANNNNNNTGG 1 cut(s) 3054
BstYI RGATCY 1 cut(s) 3271
BstZ17I GTATAC 2 cut(s) 1399, 1987
BsuRI GGCC 4 cut(s) 5, 1145, 3266, 3435
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 6 cut(s) 159, 207, 255, 303, 705, 2871
BtsCI GGATG 2 cut(s) 67, 2513
Cac8I GCNNGC 5 cut(s) 113, 919, 3080, 3084, 3088
CfoI GCGC 1 cut(s) 893
Cfr10I RCCGGY 3 cut(s) 757, 3049, 3086
CseI GACGC 1 cut(s) 961
CspAI ACCGGT 1 cut(s) 3049
CviAII CATG 4 cut(s) 909, 1036, 3354, 3396
DdeI CTNAG 4 cut(s) 1386, 1503, 1659, 3383
DpnI GATC 5 cut(s) 66, 803, 905, 932, 3273
DpnII GATC 5 cut(s) 64, 801, 903, 930, 3271
EaeI YGGCCR 2 cut(s) 3, 3433
Eam1104I CTCTTC 2 cut(s) 88, 3368
EarI CTCTTC 2 cut(s) 88, 3368
EciI GGCGGA 2 cut(s) 699, 1635
Eco105I TACGTA 2 cut(s) 1396, 1984
Eco130I CCWWGG 2 cut(s) 1011, 1044
Eco32I GATATC 1 cut(s) 854
Eco57I CTGAAG 2 cut(s) 1124, 3231
EcoRII CCWGG 1 cut(s) 3189
EcoRV GATATC 1 cut(s) 854
EcoT14I CCWWGG 2 cut(s) 1011, 1044
ErhI CCWWGG 2 cut(s) 1011, 1044
Esp3I CGTCTC 1 cut(s) 2748
FaeI CATG 4 cut(s) 912, 1039, 3357, 3399
FaqI GGGAC 2 cut(s) 2659, 3103
FatI CATG 4 cut(s) 908, 1035, 3353, 3395
FauI CCCGC 2 cut(s) 582, 3071
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 6 cut(s) 18, 713, 1060, 1263, 1272, 1298
FokI GGATG 2 cut(s) 54, 2500
Fsp4HI GCNGC 6 cut(s) 18, 713, 1060, 1263, 1272, 1298
GlaI GCGC 1 cut(s) 892
GluI GCNGC 6 cut(s) 18, 713, 1060, 1263, 1272, 1298
GsaI CCCAGC 1 cut(s) 916
GsuI CTGGAG 2 cut(s) 471, 2259
HaeII RGCGCY 1 cut(s) 894
HaeIII GGCC 4 cut(s) 5, 1145, 3266, 3435
HapII CCGG 4 cut(s) 758, 3029, 3050, 3087
HgaI GACGC 1 cut(s) 961
HhaI GCGC 1 cut(s) 893
Hin1II CATG 4 cut(s) 912, 1039, 3357, 3399
Hin6I GCGC 1 cut(s) 891
HinP1I GCGC 1 cut(s) 891
HincII GTYRAC 1 cut(s) 3060
HindII GTYRAC 1 cut(s) 3060
HinfI GANTC 5 cut(s) 3179, 3297, 3312, 3379, 3400
HpaII CCGG 4 cut(s) 758, 3029, 3050, 3087
Hpy188I TCNGA 7 cut(s) 818, 1425, 1464, 1620, 3296, 3302, 3368
Hpy188III TCNNGA 2 cut(s) 869, 884
Hpy99I CGWCG 5 cut(s) 818, 977, 1241, 3112, 3175
HpyCH4III ACNGT 6 cut(s) 147, 944, 962, 998, 1237, 2593
HpyCH4V TGCA 2 cut(s) 17, 1059
HpyF3I CTNAG 4 cut(s) 1386, 1503, 1659, 3383
Hsp92II CATG 4 cut(s) 912, 1039, 3357, 3399
HspAI GCGC 1 cut(s) 891
KroI GCCGGC 1 cut(s) 3086
KroNI GCCGGC 1 cut(s) 3088
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 5 cut(s) 64, 801, 903, 930, 3271
LmnI GCTCC 4 cut(s) 766, 1035, 1094, 1168
Lsp1109I GCAGC 2 cut(s) 29, 1071
LweI GCATC 1 cut(s) 827
MalI GATC 5 cut(s) 66, 803, 905, 932, 3273
MboI GATC 5 cut(s) 64, 801, 903, 930, 3271
MboII GAAGA 4 cut(s) 75, 995, 3210, 3355
MfeI CAATTG 1 cut(s) 1123
MflI RGATCY 1 cut(s) 3271
MhlI GDGCHC 2 cut(s) 1140, 3163
MluCI AATT 3 cut(s) 374, 965, 1123
MlyI GAGTC 2 cut(s) 3306, 3321
MmeI TCCRAC 3 cut(s) 796, 1091, 3325
MroNI GCCGGC 1 cut(s) 3086
MslI CAYNNNNRTG 1 cut(s) 3098
MspI CCGG 4 cut(s) 758, 3029, 3050, 3087
MspR9I CCNGG 1 cut(s) 3191
MunI CAATTG 1 cut(s) 1123
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 1 cut(s) 3191
MvnI CGCG 2 cut(s) 1025, 3405
NaeI GCCGGC 1 cut(s) 3088
NdeII GATC 5 cut(s) 64, 801, 903, 930, 3271
NgoMIV GCCGGC 1 cut(s) 3086
NlaIII CATG 4 cut(s) 912, 1039, 3357, 3399
NlaIV GGNNCC 6 cut(s) 762, 1009, 1041, 1096, 2841, 3091
NspI RCATGY 1 cut(s) 912
PcsI WCGNNNNNNNCGW 1 cut(s) 810
PctI GAATGC 1 cut(s) 1062
PdiI GCCGGC 1 cut(s) 3088
PfeI GAWTC 3 cut(s) 3179, 3379, 3400
PflMI CCANNNNNTGG 1 cut(s) 3431
PinAI ACCGGT 1 cut(s) 3049
PkrI GCNGC 6 cut(s) 19, 714, 1061, 1264, 1273, 1299
Ple19I CGATCG 1 cut(s) 933
PleI GAGTC 2 cut(s) 3305, 3320
PpsI GAGTC 2 cut(s) 3305, 3320
PshAI GACNNNNGTC 1 cut(s) 1212
Psp6I CCWGG 1 cut(s) 3189
PspFI CCCAGC 1 cut(s) 912
PspGI CCWGG 1 cut(s) 3189
PspN4I GGNNCC 6 cut(s) 762, 1009, 1041, 1096, 2841, 3091
PsuI RGATCY 1 cut(s) 3271
PvuI CGATCG 1 cut(s) 933
RseI CAYNNNNRTG 1 cut(s) 3098
SatI GCNGC 6 cut(s) 18, 713, 1060, 1263, 1272, 1298
Sau3AI GATC 5 cut(s) 64, 801, 903, 930, 3271
ScaI AGTACT 4 cut(s) 1818, 1952, 2087, 2222
SchI GAGTC 2 cut(s) 3306, 3321
ScrFI CCNGG 1 cut(s) 3191
SduI GDGCHC 2 cut(s) 1140, 3163
SfaNI GCATC 1 cut(s) 827
SfcI CTRYAG 2 cut(s) 106, 832
SmiMI CAYNNNNRTG 1 cut(s) 3098
SmlI CTYRAG 1 cut(s) 1139
SmoI CTYRAG 1 cut(s) 1139
SnaBI TACGTA 2 cut(s) 1396, 1984
Sse9I AATT 3 cut(s) 374, 965, 1123
StyD4I CCNGG 1 cut(s) 3189
StyI CCWWGG 2 cut(s) 1011, 1044
TaaI ACNGT 6 cut(s) 147, 944, 962, 998, 1237, 2593
TaqI TCGA 5 cut(s) 38, 885, 981, 3238, 3281
TasI AATT 3 cut(s) 374, 965, 1123
TauI GCSGC 4 cut(s) 715, 1265, 1274, 1300
TfiI GAWTC 3 cut(s) 3179, 3379, 3400
TseI GCWGC 2 cut(s) 17, 1059
TspGWI ACGGA 3 cut(s) 372, 753, 3308
Van91I CCANNNNNTGG 1 cut(s) 3431
XapI RAATTY 1 cut(s) 965
XceI RCATGY 1 cut(s) 912
XcmI CCANNNNNNNNNTGG 2 cut(s) 3051, 3171
ZrmI AGTACT 4 cut(s) 1818, 1952, 2087, 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.