Rh5AG391800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001240
Physical Location & Seq
Reverse (-)
67942072 .. 67942524
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG391800.1

Sequence Viewer

Length: 453 bp
ATGTCTCGCACAACTGGAACCCTAACGGTGCCGACTGAAGGCGTTGAACATGACGATGAAGGTCTCAGCCATCCCCTTATGACAAGCCAGGCTCATTCTGGCGTTGGCTTTCTTCCCATGATCTTTCTATGGATGTTTTGTCAAAACCGGGTTCTGATTTCGATCGGATCTCTCCCCTTCCTTATCGCAGGTCCATTCTTGAGGGTGAGTAGTTATGCTGGTAAGTGCCTGGCCTCTTGGGCTGCTGATGGTGGGACGGCGTGTGCCATCTCGGTGTGGCCTCCCTGCAATTTGGCTCGACGGTGTAAGACGGGCCTTGGAGGATTCAATTTGTTTATTCCTGATTTTGACGAAGGAGGTGCCGTGATTGGGGTGCAAGCATCGAATCATGGTGGCAATGAACAGCTAGATTGGATGGAGCTCTGCGTCGTGAGACAGAATTGTTTGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.06

Weight (kDa)

5.4

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 2 cut(s) 28, 359
AclWI GGATC 1 cut(s) 175
AcuI CTGAAG 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 38, 369
AgsI TTSAA 2 cut(s) 47, 328
AjnI CCWGG 2 cut(s) 87, 228
AluBI AGCT 2 cut(s) 406, 421
AluI AGCT 2 cut(s) 406, 421
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 3 cut(s) 9, 68, 427
AlwI GGATC 1 cut(s) 175
AoxI GGCC 3 cut(s) 231, 278, 313
ApeKI GCWGC 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 191, 313
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 1 cut(s) 191
BanI GGYRCC 2 cut(s) 28, 359
BanII GRGCYC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 1 cut(s) 229
BccI CCATC 4 cut(s) 78, 242, 275, 409
BceAI ACGGC 2 cut(s) 273, 347
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 2 cut(s) 89, 230
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 3 cut(s) 9, 68, 427
BfaI CTAG 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 3 cut(s) 89, 149, 230
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 191, 313
BmiI GGNNCC 3 cut(s) 19, 30, 361
BmrFI CCNGG 3 cut(s) 89, 149, 230
BmsI GCATC 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 1 cut(s) 149
BsaBI GATNNNNATC 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 369
Bse1I ACTGG 1 cut(s) 19
Bse3DI GCAATG 1 cut(s) 403
Bse8I GATNNNNATC 1 cut(s) 161
BseBI CCWGG 2 cut(s) 89, 230
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 3 cut(s) 70, 138, 420
BseJI GATNNNNATC 1 cut(s) 161
BseLI CCNNNNNNNGG 2 cut(s) 38, 369
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 1 cut(s) 79
BseNI ACTGG 1 cut(s) 19
BseXI GCAGC 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 3 cut(s) 233, 280, 315
BshNI GGYRCC 2 cut(s) 28, 359
BsiEI CGRYCG 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 38, 369
BsmAI GTCTC 3 cut(s) 9, 68, 427
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 3 cut(s) 233, 280, 315
Bso31I GGTCTC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 3 cut(s) 120, 162, 167
BspANI GGCC 3 cut(s) 233, 280, 315
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 19, 30, 361
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 2 cut(s) 28, 359
BspTNI GGTCTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 403
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 3 cut(s) 120, 162, 167
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 2 cut(s) 89, 230
Bst4CI ACNGT 2 cut(s) 28, 303
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 3 cut(s) 70, 138, 420
BstKTI GATC 3 cut(s) 123, 165, 170
BstMAI GTCTC 3 cut(s) 9, 68, 427
BstMBI GATC 3 cut(s) 120, 162, 167
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 2 cut(s) 89, 230
BstSCI CCNGG 3 cut(s) 87, 147, 228
BstV1I GCAGC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
BsuRI GGCC 3 cut(s) 233, 280, 315
BtsCI GGATG 3 cut(s) 70, 138, 420
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 191, 313
CseI GACGC 1 cut(s) 415
CviAII CATG 3 cut(s) 50, 118, 389
DdeI CTNAG 1 cut(s) 65
DpnI GATC 3 cut(s) 122, 164, 169
DpnII GATC 3 cut(s) 120, 162, 167
Ecl136II GAGCTC 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 87, 228
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 3 cut(s) 53, 121, 392
FaiI YATR 6 cut(s) 51, 80, 119, 130, 216, 390
FaqI GGGAC 1 cut(s) 268
FatI CATG 3 cut(s) 49, 117, 388
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 3 cut(s) 57, 145, 427
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 407
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 3 cut(s) 233, 280, 315
HapII CCGG 1 cut(s) 148
HgaI GACGC 1 cut(s) 415
Hin1II CATG 3 cut(s) 53, 121, 392
HinfI GANTC 2 cut(s) 324, 385
HpaII CCGG 1 cut(s) 148
HphI GGTGA 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 156, 167
Hpy188III TCNNGA 3 cut(s) 199, 341, 430
Hpy99I CGWCG 2 cut(s) 303, 431
HpyAV CCTTC 4 cut(s) 32, 53, 187, 347
HpyCH4III ACNGT 2 cut(s) 28, 303
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 2 cut(s) 288, 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 3 cut(s) 53, 121, 392
Kzo9I GATC 3 cut(s) 120, 162, 167
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 1 cut(s) 389
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 449
MalI GATC 3 cut(s) 122, 164, 169
MboI GATC 3 cut(s) 120, 162, 167
MboII GAAGA 1 cut(s) 104
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 3 cut(s) 289, 328, 439
MnlI CCTC 5 cut(s) 195, 244, 291, 314, 350
MslI CAYNNNNRTG 2 cut(s) 54, 272
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 3 cut(s) 89, 149, 230
MvaI CCWGG 2 cut(s) 89, 230
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 149
NdeII GATC 3 cut(s) 120, 162, 167
NlaIII CATG 3 cut(s) 53, 121, 392
NlaIV GGNNCC 3 cut(s) 19, 30, 361
PfeI GAWTC 2 cut(s) 324, 385
PkrI GCNGC 1 cut(s) 244
Ple19I CGATCG 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 423
Psp6I CCWGG 2 cut(s) 87, 228
PspGI CCWGG 2 cut(s) 87, 228
PspN4I GGNNCC 3 cut(s) 19, 30, 361
PspPI GGNCC 2 cut(s) 191, 313
PsuI RGATCY 1 cut(s) 167
PvuI CGATCG 1 cut(s) 165
RseI CAYNNNNRTG 2 cut(s) 54, 272
SacI GAGCTC 1 cut(s) 423
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 3 cut(s) 120, 162, 167
Sau96I GGNCC 2 cut(s) 191, 313
ScrFI CCNGG 3 cut(s) 89, 149, 230
SduI GDGCHC 1 cut(s) 423
SetI ASST 6 cut(s) 64, 193, 361, 408, 423, 452
SfaNI GCATC 1 cut(s) 389
SinI GGWCC 1 cut(s) 191
SmiMI CAYNNNNRTG 2 cut(s) 54, 272
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 3 cut(s) 289, 328, 439
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 1 cut(s) 423
StyD4I CCNGG 3 cut(s) 87, 147, 228
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 28, 303
TaiI ACGT 1 cut(s) 452
TaqI TCGA 3 cut(s) 161, 298, 383
TasI AATT 3 cut(s) 289, 328, 439
TfiI GAWTC 2 cut(s) 324, 385
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 2 cut(s) 72, 414
VpaK11BI GGWCC 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.